CN110553870A - system and method for collecting, classifying and counting parasite eggs - Google Patents
system and method for collecting, classifying and counting parasite eggs Download PDFInfo
- Publication number
- CN110553870A CN110553870A CN201910835745.2A CN201910835745A CN110553870A CN 110553870 A CN110553870 A CN 110553870A CN 201910835745 A CN201910835745 A CN 201910835745A CN 110553870 A CN110553870 A CN 110553870A
- Authority
- CN
- China
- Prior art keywords
- sample
- module
- eggs
- parasite
- collecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013601 eggs Nutrition 0.000 title claims abstract description 57
- 244000045947 parasite Species 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000001514 detection method Methods 0.000 claims abstract description 17
- 238000012545 processing Methods 0.000 claims abstract description 15
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 238000002347 injection Methods 0.000 claims abstract description 5
- 239000007924 injection Substances 0.000 claims abstract description 5
- 238000011161 development Methods 0.000 claims abstract description 3
- 238000004806 packaging method and process Methods 0.000 claims abstract description 3
- 238000011160 research Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 4
- 238000005086 pumping Methods 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 208000030852 Parasitic disease Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G01N2015/1022—
-
- G01N2015/1024—
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the field of parasite egg detection, and discloses a system and a method for collecting, classifying and counting parasite eggs, wherein a sample processing module is used for processing samples by arranging a special sample stirring and mixing device; the sample injection module pumps the mixed sample to a detector; the flow rate control valve is arranged, so that the system can carry out stepless speed regulation to adapt to the accurate and efficient detection work of the detector on the sample; the microscopic examination module is used for acquiring data of a sample; the shunt valve is used for sub-packaging the sample in different collection bottles; the sample collection module changes the pipeline of the detected worm eggs and accurately collects the worm eggs in different collection bottles so as to facilitate the development of subsequent analysis work; and the software control module performs comparison analysis through the acquired database, and accurately counts and classifies parasite eggs in the sample. The invention can conveniently and quickly count, classify and collect parasite eggs accurately.
Description
Technical Field
The invention belongs to the technical field of parasite egg detection, and particularly relates to a system and a method for collecting, classifying and counting parasite eggs.
Background
Currently, there are 3 main methods for detecting parasite eggs: smear, michaelis and swiss methods. The smear method is simple and easy to implement in the aspect of parasite egg detection, is suitable for detecting various parasite eggs and the like, but has low detection rate and can not be accurately quantified; the Mai's method has the qualitative and quantitative detection of the parasitosis of the small eggs in the aspect of the detection of the parasite eggs, but is only suitable for various eggs which can float by saturated saline water due to the influence of the factors such as room temperature, floating time and the like; the student method has the advantages that in the aspect of parasite egg detection, repeated or omitted counting of eggs is easy to generate; the methods have the common problem of long time consumption, if people with abundant experience want to accurately distinguish various worm eggs in excrement, people still need to identify the worm eggs by means of a map, the time consumption is long, the result is large in error, and the type judgment and the quantity measurement of various parasite eggs are seriously influenced.
In summary, the problems of the prior art are as follows:
The existing parasite egg collecting, classifying and counting system is inconvenient to collect parasite eggs, the method is long in time consumption, and the accuracy of classifying and counting is seriously reduced due to various factors such as experience in the judging process.
The difficulty of solving the technical problems is as follows:
the prior art device can accurately and efficiently collect, classify and count parasite eggs through a series of works by reasonably modifying and combining the device and special computer control software and a rich image database thereof.
The significance of solving the technical problems is as follows:
through the technical means, the method fundamentally eliminates the error estimation caused by artificial misjudgment and the systematic error caused by repeated counting and missing counting in the operation process on the classification counting of the parasite eggs and the larvae, has great significance, and can be applied to the fields of scientific research, clinical diagnosis and the like in the field of animal parasitology and even human parasitology.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a system and a method for collecting, classifying and counting parasite eggs.
The invention is realized in such a way that a system for collecting, classifying and counting parasite eggs is provided with:
The sample processing module is connected with the sample introduction module and is used for processing the sample by arranging a special sample stirring and mixing device;
The sample injection module is connected with the flow rate control valve and pumps the mixed sample to the detector;
the flow rate control valve is connected with the microscopic examination module, and the system can adjust the speed in a stepless manner by arranging the flow rate control valve so as to adapt to the accurate and efficient detection work of the detector on the sample;
The microscopic examination module is connected with the flow dividing valve and is used for acquiring data of the sample;
the shunt valve is connected with the sample collection module and is used for sub-packaging the sample in different collection bottles;
the sample collection module is used for carrying out pipeline change on the detected worm eggs and accurately collecting the worm eggs in different collection bottles so as to carry out subsequent analysis work, such as molecular biology research and the like;
The software control module is respectively connected with the sample processing module, the sample introduction module, the flow rate control valve, the microscopic examination module, the flow divider valve and the sample collection module and coordinates the normal operation of each module; meanwhile, the collected database is used for carrying out comparison analysis, and parasite eggs in the sample are accurately counted and classified.
The invention also aims to provide a method for collecting, classifying and counting parasite eggs, which specifically comprises the following steps:
Step one, arranging a special sample stirring and mixing system to process a sample;
pumping the mixed sample to a detector, wherein the system can adjust the speed in a stepless manner so as to adapt to the accurate and efficient detection work of the detector on the sample;
Thirdly, the software control system performs comparison analysis through the collected database, and accurately counts and accurately classifies parasite eggs in the sample;
Fourthly, changing the pipeline of the detected worm eggs and accurately collecting the worm eggs in different collecting bottles so as to facilitate the development of subsequent analysis work, such as molecular biological research and the like;
and step five, fully washing the sample processing system, the sample introduction system, the flow rate control valve, the microscopic examination system, the shunt valve and the auxiliary pipeline systems thereof before the sample is replaced so as to ensure the accuracy of the sample detection.
In summary, the advantages and positive effects of the invention are:
In the aspects of parasite egg collection, classification and counting, the invention can conveniently and quickly count, classify and collect parasite eggs accurately.
Drawings
FIG. 1 is a schematic structural diagram of a system for collecting, classifying and counting parasite eggs according to an embodiment of the present invention.
FIG. 2 is a flow chart of a method for collecting, classifying and counting eggs of parasite according to an embodiment of the present invention.
in the figure: 1. a sample processing module; 2. a sample introduction module; 3. a flow rate control valve; 4. a microscopic examination module; 5. a flow divider valve; 6. a sample collection module; 7. a software control module; 8. a pipeline flushing module.
Detailed Description
in order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The technical solution of the present invention is described in detail below with reference to the accompanying drawings.
As shown in FIG. 1, the system for collecting, classifying and counting parasite eggs provided by the embodiment of the invention comprises:
And the sample processing module 1 is connected with the sample injection module 2 and is used for processing the sample by arranging a special sample stirring and mixing device.
and the sample injection module 2 is connected with the flow rate control valve 3 and pumps the mixed sample to the detector.
the flow rate control valve 3 is connected with the microscopic examination module 4, and the system can carry out stepless speed regulation by being provided with the flow rate control valve 3 so as to adapt to the accurate and efficient detection work of the detector on the sample.
and the microscopic examination module 4 is connected with the flow dividing valve 5 and is used for acquiring data of the sample.
and the flow dividing valve 5 is connected with the sample collecting module 6, and the samples are subpackaged in different collecting bottles by utilizing the flow dividing valve.
And the sample collection module 6 is used for carrying out pipeline change on the detected worm eggs and accurately collecting the worm eggs in different collection bottles so as to carry out subsequent analysis work, such as molecular biology research and the like.
The software control module 7 is respectively connected with the sample processing module 1, the sample introduction module 2, the flow rate control valve 3, the microscopic examination module 4, the flow divider valve 5, the sample collection module 6 and the pipeline flushing module 8, and coordinates normal operation of each module; meanwhile, the collected database is used for carrying out comparison analysis, and parasite eggs in the sample are accurately counted and classified.
As shown in fig. 2, the method for collecting, classifying and counting parasite eggs provided by the embodiment of the present invention specifically includes:
S101: a special sample stirring and mixing system is arranged to process the sample;
S102: the mixed sample is pumped to a detector, and the system can adjust the speed in a stepless way so as to adapt to the accurate and efficient detection work of the detector on the sample;
S103: the software control system performs comparison analysis through the collected database, and accurately counts and accurately classifies parasite eggs in the sample;
s104: carrying out pipeline change on the detected worm eggs and accurately collecting the worm eggs in different collecting bottles so as to carry out subsequent analysis work, such as molecular biological research and the like;
s105: before the sample is replaced, the sample processing system, the sample introduction system, the flow rate control valve, the microscopic examination system, the shunt valve and the auxiliary pipeline system thereof are comprehensively flushed so as to ensure the accuracy of the sample detection.
the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (2)
1. a system for collecting, classifying and counting parasite eggs, wherein the system for collecting, classifying and counting parasite eggs is provided with:
the sample processing module is connected with the sample introduction module and is used for processing the sample by arranging a special sample stirring and mixing device;
The sample injection module is connected with the flow rate control valve and pumps the mixed sample to the detector;
the flow rate control valve is connected with the microscopic examination module, and the system can adjust the speed in a stepless manner by arranging the flow rate control valve so as to adapt to the accurate and efficient detection work of the detector on the sample;
The microscopic examination module is connected with the flow dividing valve and is used for acquiring data of the sample;
the shunt valve is connected with the sample collection module and is used for sub-packaging the sample in different collection bottles;
the sample collection module is used for carrying out pipeline change on the detected worm eggs and accurately collecting the worm eggs in different collection bottles so as to carry out subsequent analysis work, such as molecular biology research and the like;
The software control module is respectively connected with the sample processing module, the sample introduction module, the flow rate control valve, the microscopic examination module, the flow divider valve and the sample collection module and coordinates the normal operation of each module; meanwhile, the collected database is used for carrying out comparison analysis, and parasite eggs in the sample are accurately counted and classified.
2. a method of parasite egg collection, classification, and enumeration for a system of parasite egg collection, classification, and enumeration as claimed in claim 1, wherein the method of parasite egg collection, classification, and enumeration specifically comprises:
Step one, arranging a special sample stirring and mixing system to process a sample;
Pumping the mixed sample to a detector, wherein the system can adjust the speed in a stepless manner so as to adapt to the accurate and efficient detection work of the detector on the sample;
Thirdly, the software control system performs comparison analysis through the collected database, and accurately counts and accurately classifies parasite eggs in the sample;
Fourthly, changing the pipeline of the detected worm eggs and accurately collecting the worm eggs in different collecting bottles so as to facilitate the development of subsequent analysis work, such as molecular biological research and the like;
And step five, fully washing the sample processing system, the sample introduction system, the flow rate control valve, the microscopic examination system, the shunt valve and the auxiliary pipeline systems thereof before the sample is replaced so as to ensure the accuracy of the sample detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910835745.2A CN110553870A (en) | 2019-09-05 | 2019-09-05 | system and method for collecting, classifying and counting parasite eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910835745.2A CN110553870A (en) | 2019-09-05 | 2019-09-05 | system and method for collecting, classifying and counting parasite eggs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110553870A true CN110553870A (en) | 2019-12-10 |
Family
ID=68739121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910835745.2A Pending CN110553870A (en) | 2019-09-05 | 2019-09-05 | system and method for collecting, classifying and counting parasite eggs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110553870A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1773290A (en) * | 2005-10-22 | 2006-05-17 | 重庆天海医疗设备有限公司 | Arena, fecal and hydrocephalus microscopy sampler feed and cleaning method |
| CN201060190Y (en) * | 2007-06-12 | 2008-05-14 | 合肥新月电子技术有限责任公司 | Full-automatic dejecta analytical equipment |
| WO2008064442A2 (en) * | 2006-11-30 | 2008-06-05 | Universidade Estadual De Campinas - Unicamp | A system for diagnosing intestinal parasites by computerized image analysis |
| CN101407759A (en) * | 2008-11-28 | 2009-04-15 | 北京理工大学 | Blood cell analyzer and analysis method |
| CN101487845A (en) * | 2009-02-19 | 2009-07-22 | 上海吉鸿生物科技有限公司 | Automatic detection instrument for excrement sample |
| CN102393705A (en) * | 2011-10-18 | 2012-03-28 | 长沙高新技术产业开发区爱威科技实业有限公司 | Sample formed component analyzer automatic detection control apparatus and control method thereof |
| CN103034867A (en) * | 2011-09-29 | 2013-04-10 | 蔡宏营 | Insect image detection method and insect classification method |
| CN203117207U (en) * | 2013-02-04 | 2013-08-07 | 爱威科技股份有限公司 | Multi-module microscopic examination detecting system |
| CN105988208A (en) * | 2015-02-02 | 2016-10-05 | 长沙明鹏科技有限公司 | Online microscopic method and device of sewage |
| CN109283181A (en) * | 2018-11-27 | 2019-01-29 | 东莞宽谱光电科技有限公司 | A kind of excrement detection system and detection method |
-
2019
- 2019-09-05 CN CN201910835745.2A patent/CN110553870A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1773290A (en) * | 2005-10-22 | 2006-05-17 | 重庆天海医疗设备有限公司 | Arena, fecal and hydrocephalus microscopy sampler feed and cleaning method |
| WO2008064442A2 (en) * | 2006-11-30 | 2008-06-05 | Universidade Estadual De Campinas - Unicamp | A system for diagnosing intestinal parasites by computerized image analysis |
| CN201060190Y (en) * | 2007-06-12 | 2008-05-14 | 合肥新月电子技术有限责任公司 | Full-automatic dejecta analytical equipment |
| CN101407759A (en) * | 2008-11-28 | 2009-04-15 | 北京理工大学 | Blood cell analyzer and analysis method |
| CN101487845A (en) * | 2009-02-19 | 2009-07-22 | 上海吉鸿生物科技有限公司 | Automatic detection instrument for excrement sample |
| CN103034867A (en) * | 2011-09-29 | 2013-04-10 | 蔡宏营 | Insect image detection method and insect classification method |
| CN102393705A (en) * | 2011-10-18 | 2012-03-28 | 长沙高新技术产业开发区爱威科技实业有限公司 | Sample formed component analyzer automatic detection control apparatus and control method thereof |
| CN203117207U (en) * | 2013-02-04 | 2013-08-07 | 爱威科技股份有限公司 | Multi-module microscopic examination detecting system |
| CN105988208A (en) * | 2015-02-02 | 2016-10-05 | 长沙明鹏科技有限公司 | Online microscopic method and device of sewage |
| CN109283181A (en) * | 2018-11-27 | 2019-01-29 | 东莞宽谱光电科技有限公司 | A kind of excrement detection system and detection method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102393705B (en) | Sample formed component analyzer automatic detection control apparatus and control method thereof | |
| CN102662048B (en) | Method and device for analyzing urine | |
| CN202770842U (en) | Urine analysis device | |
| JP2018505392A (en) | Automated flow cytometry analysis method and system | |
| CN110473167B (en) | Deep learning-based urinary sediment image recognition system and method | |
| CN107490672B (en) | method for rapidly analyzing crustacean hemolymph cell group and number and application | |
| JP7352365B2 (en) | Cell analysis method, deep learning algorithm training method, cell analysis device, deep learning algorithm training device, cell analysis program, and deep learning algorithm training program | |
| WO2012139047A2 (en) | Identifying and enumerating early granulated cells (egcs) | |
| CN103345654A (en) | Method for differential counting of white blood cells based on morphology | |
| CN112161913A (en) | Analysis method and equipment for flow type fluorescence analysis system | |
| CN112001315B (en) | Bone marrow cell classification and identification method based on migration learning and image texture characteristics | |
| US11123734B2 (en) | System and method for immune activity determination | |
| CN102636656B (en) | Calibration method of full-automatic urine visible component analyser | |
| CN1904618A (en) | Method of implementing erythrocyte blood group antigen detection on haemocyte analysis instrument | |
| CN201060190Y (en) | Full-automatic dejecta analytical equipment | |
| Ahmed et al. | Morphometric measurements of blood cell | |
| CN107686859B (en) | Method suitable for rapid classified counting of fish blood cells and application | |
| CN110553870A (en) | system and method for collecting, classifying and counting parasite eggs | |
| CN102375073A (en) | Fully automatic fluorine analyzer and analytical method thereof | |
| CN110277139B (en) | Microorganism limit checking system and method based on Internet | |
| CN101510235A (en) | Blight hemagglutinin detecting analysis system | |
| CN114152557B (en) | Image analysis-based blood cell counting method and system | |
| CN202794194U (en) | Comprehensive sample analyzer | |
| CN202257125U (en) | Automatic detection controller for sample visible component analytical instrument | |
| CN104700422A (en) | Method for automatically segmenting bonded red blood cells and white blood cells in urinary sediment image |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |