CN110537545A - Application of tioconazole in preparing bactericide for preventing and treating plant pathogenic bacteria - Google Patents
Application of tioconazole in preparing bactericide for preventing and treating plant pathogenic bacteria Download PDFInfo
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- CN110537545A CN110537545A CN201910868201.6A CN201910868201A CN110537545A CN 110537545 A CN110537545 A CN 110537545A CN 201910868201 A CN201910868201 A CN 201910868201A CN 110537545 A CN110537545 A CN 110537545A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
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Abstract
The invention discloses an application of tioconazole in preparing bactericide for preventing and treating plant pathogenic bacteria. According to the invention, indoor reagent sensitivity determination proves that tioconazole has good inhibitory activity on main diseases of important grain crops, including fusarium graminearum causing wheat scab, curvularia zea causing corn leaf spot, rhizoctonia cerealis causing sheath blight and pyricularia oryzae causing rice blast; but also has good inhibitory activity to botrytis cinerea which causes the main disease of fruits and vegetables, ring spot of apple and alternaria tenuissima which causes leaf spot of fruits and vegetables; but also has better inhibitory activity to soil-borne diseases which are extremely difficult to be controlled in agricultural production, such as fusarium oxysporum which causes blight, and the bacteriostatic activity of the compound is even superior to that of the traditional bactericides carbendazim and tebuconazole. The invention firstly provides the application of tioconazole in the prevention and control of important plant diseases in agricultural production, and has wide market application prospect.
Description
Technical Field
The invention belongs to the technical field of plant protection, and particularly relates to application of tioconazole in preparation of a bactericide for preventing and treating phytopathogen.
Background
The united nations Food and Agriculture Organization (FAO) estimates that the influence of the yield of crops on trees is difficult to estimate due to about 40 percent loss caused by the damage of pests and weeds (4400-5500 billion yuan). The loss of work recovery of scientists and various technical popularization personnel engaged in pest and disease research worldwide reaches 3000-. Chemical control has long been the primary means of controlling plant diseases. The long-term use of a large amount of chemical pesticides not only causes resistance problems, but also causes medicament residues, kills non-target organisms, pollutes the ecological environment and finally causes harm to human bodies through biological enrichment. In order to solve the problems, the design and screening of green small molecular compounds by using the three-dimensional structure of the plant pathogenic microorganism target protein becomes a research hotspot.
In recent years, environmental safety issues have attracted attention from people in all fields, and environmental pollution, residue, drug resistance and the like have generated great interest in green small molecule compounds. Therefore, sustainable development of agriculture needs a new and safe measure for controlling plant diseases. Under such a large environment, development and effective utilization of a novel, environmentally friendly bactericide are urgently required.
Disclosure of Invention
The invention aims to provide application of tioconazole in preparing a bactericide for preventing and treating phytopathogen. The invention provides a method for screening a green small molecular compound tioconazole by using a target protein three-dimensional structure to be applied to the prevention and control of plant diseases, the bacteriostatic activity of the tioconazole is superior to that of the traditional bactericides carbendazim and tebuconazole, and the method is environment-friendly and has wide market application prospect.
In order to realize the purpose of the invention, the invention adopts the following technical scheme to realize:
The invention provides application of tioconazole in preparing a bactericide for preventing and treating phytopathogen.
Further: the plant pathogenic bacteria are fusarium graminearum, curvularia zeae, ring rot of apple, alternaria tenuis, fusarium oxysporum, rice blast, rhizoctonia cerealis and botrytis cinerea.
Further: the using concentration of the tioconazole in the bactericide is 0.1mg/L-2 mg/L.
Further: the sterilization inhibition rate of the tioconazole to the fusarium graminearum is 53-69%.
Further: the sterilization inhibition rate of the tioconazole to the curvularia zeae is 46-81%.
Further: the using concentration of the tioconazole to the ring rot apple bacteria is 0.1mg/L-1mg/L, and the sterilization inhibition rate is 28% -100%.
Further: the inhibition rate of the tioconazole to Alternaria tenuissima is 31-70%.
Further: the inhibition rate of the tioconazole to fusarium oxysporum is 21% -43%, and the inhibition rate of the tioconazole to rice blast bacteria is 12% -34%.
Further: the inhibition rate of the tioconazole to the rhizoctonia cerealis is 42% -68%, and the inhibition rate of the tioconazole to the botrytis cinerea is 31% -82%.
Further: the plant is selected from wheat, corn, rice, apple, melon, Solanaceae, banana, cotton, Leguminosae, green pepper, celery, lettuce and grape.
Compared with the prior art, the invention has the advantages and the technical effects that: the invention screens and synthesizes the small molecular compound tioconazole, and the invention proves that the tioconazole has good inhibition activity on main diseases of important grain crops, including fusarium graminearum causing wheat scab, curvularia zea causing corn leaf spot, rhizoctonia cerealis causing sheath blight and magnaporthe grisea causing rice blast, through indoor reagent sensitivity determination; but also has good inhibitory activity to botrytis cinerea which causes the main disease of fruits and vegetables, ring spot of apple and alternaria tenuissima which causes leaf spot of fruits and vegetables; but also has better inhibitory activity to soil-borne diseases which are extremely difficult to be controlled in agricultural production, such as fusarium oxysporum which causes blight, and the bacteriostatic activity of the compound is even superior to that of the traditional bactericides carbendazim and tebuconazole.
The invention provides a green micromolecular compound tioconazole screened by a target protein three-dimensional structure for preventing and controlling plant diseases, which can effectively solve the problem of poor prevention effect caused by the resistance of plant pathogenic fungi such as fusarium graminearum and ring rot to the existing bactericide, is environment-friendly, green and pollution-free, meets the drug residue toxicity standard of the world health organization, and has wide market application prospect.
drawings
FIG. 1 is a graph showing the effect of different concentration gradients of tioconazole and the effect of traditional medicaments carbendazim and tebuconazole on the growth of fusarium graminearum hyphae.
FIG. 2 is a graph showing the effect of different concentration gradients of tioconazole and the effect of traditional medicines carbendazim and tebuconazole on the growth of Curvularia lunata hypha.
FIG. 3 is a graph showing the effect of different concentration gradients of tioconazole and the effect of traditional agents carbendazim and tebuconazole on inhibiting the growth of hypha of ring rot of apple.
FIG. 4 is a graph showing the effect of different concentration gradients of tioconazole and the effect of conventional agents carbendazim and tebuconazole on the growth of alternaria tenuissima filaments.
FIG. 5 is a graph showing the effect of different concentration gradients of tioconazole and the effect of conventional carbendazim and tebuconazole on the growth of fusarium oxysporum hyphae.
FIG. 6 is a graph showing the inhibitory effect of tioconazole with different concentration gradients and traditional agents carbendazim and tebuconazole on the growth of rice blast fungus hypha.
FIG. 7 is a graph showing the effect of different concentration gradients of tioconazole and the effect of conventional agents carbendazim and tebuconazole on the growth of sclerotium rolfsii hyphae.
FIG. 8 is a graph showing the effect of different concentration gradients of tioconazole and the effect of conventional agents carbendazim and tebuconazole on the growth of botrytis cinerea hyphae.
Wherein CK is a negative control; 0.1, 0.3, 0.5, 1.0 and 2.0 represent tioconazole with different concentration gradients, and the unit is mg/l; DJL0.6 and WZC0.125 are used as positive control, namely, carbendazim 0.6mg/l and tebuconazole 0.125 mg/1.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the accompanying drawings and examples.
Example 1
the invention screens and synthesizes a small molecular compound tioconazole in an InterBioScreen database (https:// www.ibscreen.com /).
Chemical name: 1- [2- [ (2-chloro-3-thienyl) methoxy ] -2- (2, 4-dichlorophenyl) ethyl ] -1H-imidazole english: 1- (2- ((2-chloro-3-thienyl) methoxy) -2- (2, 4-dichlorophenyl) ethyl) -1h-imidazol, molecular formula: C16H13CL3N2OS, molecular weight: 387.71, the structural formula is as follows:
1. Test method
The drug sensitivity of tioconazole to plant pathogenic bacteria is measured by colony growth rate inhibition method. The growth rate method is also called as a medium-containing method, is particularly suitable for test strains which do not produce spores on a culture medium or produce few spores and have dense hyphae, and meets the requirements of the experiment.
The experimental procedure was as follows:
(1) Activating the strain: inoculating 8 plant pathogenic fungi such as Fusarium graminearum, Curvularia zeae, ring rot of apple, Alternaria tenuis, Fusarium oxysporum, Magnaporthe grisea, Rhizoctonia cerealis and Botrytis cinerea in the storage tube on a PDA plate, culturing for 3-4 days, and placing in a constant temperature refrigerator at 4 ℃ for later use.
(2) Preparing a mother solution: 0.0101g of tioconazole, carbendazim and tebuconazole are respectively weighed and prepared into mother liquor with the concentration of 1 x 104mg/l by using a solvent of dimethyl sulfoxide (DMSO).
(3) Preparing a toxic flat plate: heating PDA culture medium to melt, cooling to 45-50 deg.C, adding mother liquor to obtain culture medium containing 0.1mg/l, 0.3mg/l, 0.5mg/l, 1.0mg/l and 2.0mg/l medicinal liquid (adding corresponding volume of dimethyl sulfoxide), mixing, and cooling in culture dish. Each treatment was set to 3 replicates.
(4) And (3) inoculating pathogenic bacteria: the bacterial cake is punched on the same circumference of the edge of the pre-cultured bacterial colony by a puncher (the diameter is 6mm), the bacterial cake is connected to the center of the toxic plate, and the bacterial cake is sealed and then placed in a constant temperature incubator at 25 ℃ for culture.
2. Data processing
When the colony edge of the control group is close to the dish wall, the colony diameter of each treatment group is measured by adopting a cross method, and the growth inhibition rate is calculated.
3. Test results
The effects of tioconazole on the growth rate of fusarium graminearum, curvularia zeae, ring rot apple, alternaria tenuis, fusarium oxysporum, magnaporthe oryzae, rhizoctonia cerealis and botrytis cinerea colonies (fig. 1-8), and the concentrations and inhibition rates of the test agents are shown in table 1.
TABLE 1 inhibition of tioconazole on the growth of 8 pathogenic bacteria hypha
As can be seen from the experimental results of Table 1 and FIGS. 1-8, the tioconazole compound of the present invention has significant inhibitory effect on Fusarium graminearum, Curvularia zeae, Verticillium Mali Pumilae, Alternaria tenuissima, Fusarium oxysporum, Magnaporthe grisea, Rhizoctonia cerealis and Botrytis cinerea. And in the range of 0.1mg/L-2mg/L, the inhibition effect is more obvious along with the increase of the drug concentration.
(1) Wheat scab: scab is a devastating disease that can cause ear rot, resulting in severe yield loss and quality loss. With the change of global warming, cultivation system and mode, wheat scab is spread and expanded continuously, which often causes the yield reduction and quality reduction of wheat, and infected wheat grains contain mycotoxin, which can cause poisoning and serious diseases of people and livestock. The germs not only harm wheat, but also can infect various gramineous crops such as barley, oat, rice, corn and the like, gramineous weeds such as coronarium sinense and the like, and can infect crops such as soybean, cotton, sweet potato and the like. At present, main chemical agents for preventing and controlling wheat scab comprise carbendazim, tebuconazole and the like, in an experiment, the inhibition rate of 0.1mg/l of tioconazole to fusarium graminearum is 53.35%, the inhibition rate reaches 69.37% with the concentration increased to 2mg/l, and the inhibition effect is good.
(2) Curvularia zeae: curvularia zeae can cause leaf spot disease in corn, which has been called "maculopathy" according to its symptoms, mainly damaging plant leaves, and also damaging leaf sheaths and bracts. The corn leaf spot is one of the most main diseases in the corn production process, the disease generally reduces the yield of the corn by 20 to 30 percent, and the individual plot reaches more than 50 percent, even the corn is out of production. In the experiment, the inhibition rate of 0.1mg/l of tioconazole to Curvularia lunata is 46.33%, the inhibition rate of 0.125mg/l of tebuconazole to Curvularia lunata is 18.67%, and the effect of tioconazole is obviously superior to that of the traditional azole medicament tebuconazole and the traditional benzimidazole medicament carbendazim.
(3) Apple ring rot bacteria: apple ring rot, also known as rough skin disease and ring rot, is an important biological disaster on branches and trunks and fruits of apples, and often causes rough bark, local necrosis and fruit rot of the branches and the trunks of the apples. The fruit setting rate of the diseased plants is low, which causes the trees to be weak and the yield to be reduced, and even the plants are dead to produce and damage gardens. In recent years, with the large-area cultivation of the Fuji apple which is a susceptible variety, the incidence rate of ring rot of the apple is increased year by year, the area of damage is continuously enlarged, the ring rot of the apple becomes a serious disease in the production of the apple in China, and the sustainable development of the apple industry is seriously threatened. In the experiment, the inhibition rate of 0.5mg/l of thiaconazole to ring rot of apple is 86.21%, the inhibition rate of 0.6mg/l of carbendazim to ring rot of apple is 81.82%, and the effect of the thiaconazole is obviously superior to that of the traditional azole carbendazim.
(4) Alternaria tenuissima: alternaria tenuissima is a pathogenic fungus capable of infecting various food crops, economic crops, fruits and vegetables, and is also one of economically important fungi. Most species are facultative parasitic on plants, causing a variety of economic plant diseases, causing field and postpartum losses. In the experiment, the bacteriostasis rate of 0.5mg/l of tioconazole to alternaria tenuissima is 52.46 percent, while the bacteriostasis rate of 0.6mg/l of carbendazim to alternaria tenuissima is 0.25 percent, and the effect of tioconazole is obviously superior to that of the traditional medicament carbendazim. In the experiment, the bacteriostasis rate of 0.1mg/l tioconazole to alternaria tenuissima is 31.15 percent, the bacteriostasis rate of 0.125mg/l tebuconazole to alternaria tenuissima is 0.13 percent, and the effect of tioconazole is obviously superior to that of the traditional medicament tebuconazole.
(5) Fusarium oxysporum: fusarium oxysporum is a kind of facultative parasitic fungus which can infect plants and can be distributed worldwide in soil, has a wide host range, and can cause blight of more than 100 plants such as melons, solanaceae, bananas, cotton, leguminous plants, flowers and the like. In the experiment of the invention, the inhibition rate of 1mg/1 tioconazole to fusarium oxysporum is 33.25%, the inhibition rate of 2mg/l tioconazole to fusarium oxysporum is 43.50%, and tioconazole has good inhibition effect to fusarium oxysporum.
(6) Rice blast germs: the rice blast is one of important diseases of rice, can cause great yield reduction, and can reduce the yield by 40 to 50 percent even if the yield is serious, and even no grains are harvested. All rice areas in the world occur uniformly. This disease occurs in all regions, with more occurrence of leaves and nodes, which may cause different degrees of reduction in yield after occurrence, especially early and severe neck blast or node blast, which may cause white ears and loss of yield. In the experiment of the invention, the inhibition rate of 1mg/l of tioconazole on rice blast bacteria is 25.48%, the inhibition rate of 2mg/l of tioconazole on rice blast bacteria is 34.61%, and the tioconazole has good inhibition effect on the rice blast bacteria.
(7) Rhizoctonia cerealis: rhizoctonia cerealis is a soil-borne pathogenic fungus that causes sheath blight of wheat, barley, corn, and rice. The Rhizoctonia cerealis exists in the form of hypha and sclerotium, does not produce any conidia, and has slow growth and light color. In the experiment, the bacteriostasis rate of 0.1mg/l of tioconazole to rhizoctonia cerealis is 42.47%, the bacteriostasis rate of 0.125mg/l of tebuconazole to rhizoctonia cerealis is 0.28%, and the effect of tioconazole is obviously superior to that of the traditional medicament tebuconazole.
(8) botrytis cinerea: the botrytis cinerea has wide hosts and can infect a plurality of fruits and vegetables such as tomatoes, cucumbers, eggplants, beans, green peppers, celery, asparagus lettuce, grapes and the like. In the experiment of the invention, the bacteriostasis rate of 0.5mg/l of tioconazole to botrytis cinerea is 55.7 percent, the bacteriostasis rate of 0.6mg/l of carbendazim to botrytis cinerea is 15.72 percent, and the effect of tioconazole is obviously superior to that of the traditional medicament carbendazim.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (10)
1. The application of tioconazole in preparing bactericide for preventing and treating plant pathogenic bacteria.
2. The use of tioconazole according to claim 1 in the preparation of fungicides for controlling phytopathogens, characterized in that: the plant pathogenic bacteria are fusarium graminearum, curvularia zeae, ring rot of apple, alternaria tenuis, fusarium oxysporum, rice blast, rhizoctonia cerealis and botrytis cinerea.
3. The use of tioconazole according to claim 1 or 2 for the preparation of fungicides for the control of phytopathogens, characterized in that: the using concentration of the tioconazole in the bactericide is 0.1mg/L-2 mg/L.
4. The use of tioconazole according to claim 3 in the preparation of fungicides for controlling phytopathogens, characterized in that: the sterilization inhibition rate of the tioconazole to the fusarium graminearum is 53-69%.
5. The use of tioconazole according to claim 3 in the preparation of fungicides for controlling phytopathogens, characterized in that: the sterilization inhibition rate of the tioconazole to the curvularia zeae is 46-81%.
6. The use of tioconazole according to claim 3 in the preparation of fungicides for controlling phytopathogens, characterized in that: the using concentration of the tioconazole to the ring rot apple bacteria is 0.1mg/L-1mg/L, and the sterilization inhibition rate is 28% -100%.
7. The use of tioconazole according to claim 3 in the preparation of fungicides for controlling phytopathogens, characterized in that: the inhibition rate of the tioconazole to Alternaria tenuissima is 31-70%.
8. The use of tioconazole according to claim 3 in the preparation of fungicides for controlling phytopathogens, characterized in that: the inhibition rate of the tioconazole to fusarium oxysporum is 21% -43%, and the inhibition rate of the tioconazole to rice blast bacteria is 12% -34%.
9. The use of tioconazole according to claim 3 in the preparation of fungicides for controlling phytopathogens, characterized in that: the inhibition rate of the tioconazole to the rhizoctonia cerealis is 42% -68%, and the inhibition rate of the tioconazole to the botrytis cinerea is 31% -82%.
10. The use of tioconazole according to claim 3 in the preparation of fungicides for controlling phytopathogens, characterized in that: the plant is selected from wheat, corn, rice, apple, melon, Solanaceae, banana, cotton, Leguminosae, green pepper, celery, lettuce and grape.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4062966A (en) * | 1975-04-30 | 1977-12-13 | Pfizer Inc. | 1-Aryl-2-(1-imidazolyl) alkyl ethers and thioethers |
| WO2000028821A1 (en) * | 1998-11-16 | 2000-05-25 | Astan, Inc. | Therapeutic anti-fungal nail preparation |
-
2019
- 2019-09-12 CN CN201910868201.6A patent/CN110537545A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4062966A (en) * | 1975-04-30 | 1977-12-13 | Pfizer Inc. | 1-Aryl-2-(1-imidazolyl) alkyl ethers and thioethers |
| WO2000028821A1 (en) * | 1998-11-16 | 2000-05-25 | Astan, Inc. | Therapeutic anti-fungal nail preparation |
Non-Patent Citations (4)
| Title |
|---|
| ALI M.M.SHOMEET 等: "Effect of fungicides, antioxidants and their mixtures on mycelial growth of some fungi", 《MIDDLE EAST JOURNAL OF APPLIED SCIENCES》 * |
| GIOVANNA SIMONETTI等: "Increase of acti v ity of tioconazole against resistant microorganisms by the addition of butylated hydroxyanisole", 《INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS》 * |
| MARTIN ŽABKA等: "Effectiveness of Environmentally Safe Food Additives and Food Supplements in an In Vitro Growth Inhibition of Significant Fusarium, Aspergillus and Penicillium species", 《PLANT PROTECT. SCI.》 * |
| S. BELGHIT ET AL.: "Activity of 2,4-Di-tert-butylphenol produced by a strain of Streptom yces mutabilis isolated from a Saharan soil against Candida albicans and other pathogenic fungi", 《 JOURNAL DE MYCOLOGIE MÉDICALE》 * |
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