CN110530780A - A kind of cell hyperbaric chamber being equipped on microscopic system - Google Patents
A kind of cell hyperbaric chamber being equipped on microscopic system Download PDFInfo
- Publication number
- CN110530780A CN110530780A CN201910813452.4A CN201910813452A CN110530780A CN 110530780 A CN110530780 A CN 110530780A CN 201910813452 A CN201910813452 A CN 201910813452A CN 110530780 A CN110530780 A CN 110530780A
- Authority
- CN
- China
- Prior art keywords
- cabin
- hyperbaric chamber
- limition orientation
- pedestal
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
Abstract
The present invention relates to a kind of cell hyperbaric chambers for being equipped on microscopic system, it is characterised in that: including hyperbaric chamber, cabin pedestal, relief pipeline and stabilization clamp holder;By being provided with the high pressure cabin structure of visualization structure on microscope carrier, the hyperbaric environment of cell can be formed directly on microscope carrier, dynamic observation is carried out to cell in direct voltage environment;The problem of avoiding and take out cell under hyperbaric environment, cell is caused to change at a high pressure condition rapidly, the physiological signal captured is caused to be delay;Hyperbaric chamber is compressed by the way that stabilization clamp holder and guide post structure are arranged on cabin pedestal, quick location and installation and disassembly are convenient for using this structure;Guide holder is set in bottom closure flap; using the contact of guide seat structure and guide post effectively prevent limition orientation hole be aligned with guide post it is devious simultaneously, also avoid effective protection of the limition orientation column directly with the contact of limition orientation plate realization to bottom cover plate upper limit guide plate.
Description
Technical field
The present invention relates to hyperbaric chamber technical field more particularly to a kind of cell hyperbaric chambers for being equipped on microscopic system.
Background technique
In recent years, the increasingly attention with country to ocean development, diving activity increasingly increase, and study hyperbar to human body
The influence of physiological function such as inert gas narcosis, high pressure nervous syndrome and hyperbaric oxygen exposure etc. become concerned by people heavy
Point.But the research in the field still has many technical problems, as we want to observe hyperbar to the cell and tissue of culture
Influence, it is necessary first to by the cell or tissue of culture be placed in dedicated high atmospheric pressure cabin or work station carry out hyperbar exposure,
It is to be exposed to terminate and be further taken out after depressurizing after a period of time, then it is immediately placed in the variation of microscopically observation indices,
Since certain functions of cell change rapidly at a high pressure condition, and these variations disappear quickly after leaving hyperbaric environment
It loses, therefore is delay using the physiological signal that existing method captures, even wrong, these defects can all produce experiment
It is raw to mislead.
Currently, above-mentioned problem can be overcome there is no this device, and this can be equipped on microscope carrier simultaneously using us
Above-mentioned problem can fundamentally be solved using the equipment of microscope dynamic observation, the present apparatus both can for culture tissue or
Cell provides the pressure environment at a deep-sea, and may be implemented under hyperbar exposure and utilize the long time-histories dynamic observation culture of microscope
Tissue and cell indices, improve the accuracy of scientific data.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of cell hyperbaric chamber for being equipped on microscopic system, Neng Gouti
The accuracy of cell observation under high hyperbaric environment.
In order to solve the above technical problems, the present invention provides a kind of cell hyperbaric chamber for being equipped on microscopic system, it is described
Cell hyperbaric chamber is equipped on the objective table of microscopic system, it is characterised in that: including hyperbaric chamber, cabin pedestal, relief pipeline
With stabilization clamp holder;
The hyperbaric chamber includes high pressure cabin, top blind flange, top observation window, bottom cover plate and bottom observation window;It is described
High pressure cabin accommodates the cylindrical chamber of culture solution and glass slide, the cylindrical chamber in being equipped in bar shape structure and hyperbaric chamber body
High pressure cabin both ends are connected;Air inlet and exhaust outlet are provided on the side of the high pressure cabin;The top blind flange
The top observation window and the bottom observation window are pressed on to the both ends of high pressure cabin respectively with bottom cover plate;
Several location holes are provided on the cabin pedestal, the cabin pedestal is locked at aobvious by the location hole by bolt
Follow objective table mobile on the objective table of micromirror systems;The guiding with the cooperation of hyperbaric chamber bottom end is provided on the cabin pedestal
Column, the guide post are stepped shaft structure;Edge in the bottom cover plate of the hyperbaric chamber is provided with limition orientation plate and limit
Limition orientation hole is provided on guide plate, high pressure is realized in the cascaded surface cooperation of the guide post on the limition orientation hole and cabin pedestal
The limition orientation in cabin, the guide post structure of the ladder shaft type to be formed between light transmission between cabin pedestal and the bottom end of hyperbaric chamber
Gap;
The relief pipeline is connected on the exhaust outlet of high pressure cabin, is provided with relief valve and pressure on the relief pipeline
Monitor table;
The stabilization clamp holder is arranged on cabin pedestal and is pressed on cabin pedestal positioned at the bottom end surrounding of hyperbaric chamber
Guide post cascaded surface on.
Further, the stabilization clamp holder includes firm banking, hinged pedestal, pressing handle and compact heap;It is described solid
Determine the surrounding that pedestal is vertically set on cabin pedestal and is distributed in hyperbaric chamber;The top of firm banking is arranged in the hinged pedestal
End, the pressing handle and the orthogonal setting of compact heap form L-type structure, and one end of the pressing handle is hinged on hinged base
On seat, it is pressed in compact heap on limition orientation plate by rotating pressing handle.
Further, a limition orientation seat is provided in the limition orientation hole on the limition orientation plate;The limit is led
It is the cylindrical tube structure of a pair of of coaxial arrangement to seat, and a wherein cylindrical tube structure for limition orientation seat is embedded in limit and leads
Into hole, another cylindrical tube structure of the limition orientation seat is pressed on the lower surface of limition orientation plate;The limition orientation
Column is embedded in limition orientation seat.
Further, the top observation window and bottom observation window are the glass structure of blue coating film treatment.
Further, bolt hole, top blind flange are provided on the edge of the top blind flange and the edge of bottom cover plate
Pass through bolt respectively with bottom cover plate to be locked on the bulkhead of high pressure cabin, the top blind flange and bottom cover plate and hyperbaric chamber
The contact position of the end of body is provided with O-ring seal.
Compared with prior art, the present invention has following technical effect that
1) can directly be existed in the present invention by being provided with the high pressure cabin structure of visualization structure on microscope carrier
The hyperbaric environment that cell is formed on microscope carrier, carries out dynamic observation to cell directly in hyperbaric environment;Avoid from
Cell is taken out under hyperbaric environment, causes certain functions of cell to change at a high pressure condition rapidly, leads to the physiology captured
Signal is delay, even wrong situation.
2) hyperbaric chamber is compressed by the way that stabilization clamp holder and guide post structure are arranged on cabin pedestal in the present invention, used
This structure is convenient for quick location and installation and disassembly;Guide holder is set in bottom closure flap, is connect using guide seat structure and guide post
Touching effectively prevent limition orientation hole be aligned with guide post it is devious simultaneously, also avoid limition orientation column directly with limition orientation plate
Contact realize to the effective protection of bottom cover plate upper limit guide plate.
Detailed description of the invention
Fig. 1 is a kind of overlooking structure figure of cell hyperbaric chamber for being equipped on microscopic system of the invention.
Fig. 2 is a kind of side structure schematic view of cell hyperbaric chamber pair for being equipped on microscopic system of the invention.
Appended drawing reference: 1. hyperbaric chambers;11. high pressure cabin;12. top blind flange;13. top observation window;14. bottom cover plate;
15. bottom observation window;2. cabin pedestal;21. guide post;22. limition orientation plate;23. limition orientation seat;3. relief pipeline;31.
Relief valve;32. pressure monitoring table;4. stabilization clamp holder;41. firm banking;42. hinged pedestal;43. pressing handle;44. compressing
Block.
Specific embodiment
For a further understanding of the present invention, the preferred embodiment of the invention is described below with reference to embodiment, still
It should be appreciated that these descriptions are intended merely to further illustrate the features and advantages of the present invention, rather than to the claims in the present invention
Limitation.
A kind of cell hyperbaric chamber being equipped on microscopic system as shown in Figure 1, 2, the hyperbaric chamber are equipped on microscope system
On the objective table of system;Including hyperbaric chamber 1, cabin pedestal 2, relief pipeline 3 and stabilization clamp holder 4.
Hyperbaric chamber 1 includes high pressure cabin 11, top blind flange 12, top observation window 13, bottom cover plate 14 and bottom observation window
15;High pressure cabin 11 accommodates the cylindrical chamber of culture solution and glass slide, cylinder in being provided in bar shape structure and high pressure cabin 11
11 both ends of high pressure cabin are connected chamber;Air inlet and exhaust outlet are provided on the side of high pressure cabin 11;Top blind flange 12 with
Top observation window 13 and bottom observation window 15 are pressed on the both ends of high pressure cabin 11 by bottom cover plate 14 respectively;Top observation window
13 with bottom observation window 14 be blue coating film treatment glass structure;On the edge of top blind flange 12 and the edge of bottom cover plate 14
It is provided with bolt hole, top blind flange 12 and bottom cover plate 14 are locked on the bulkhead of high pressure cabin 11 respectively by bolt, are pushed up
The contact position of the end of portion's cover board 12 and bottom cover plate 14 and high pressure cabin 11 is provided with O-ring seal.
Several location holes are provided on cabin pedestal 2, cabin pedestal 2 is locked at microscopic system by bolt by location hole
Follow objective table mobile on objective table;The guide post 21 with the cooperation of 1 bottom end of hyperbaric chamber, guide post 21 are provided on cabin pedestal 2
For stepped shaft structure;Edge in the bottom cover plate 14 of hyperbaric chamber 1 is provided on limition orientation plate 22 and limition orientation plate 22 and opens
There is limit pilot hole, limition orientation hole and the cascaded surface cooperation of the guide post 21 on cabin pedestal 2 realize that the limit of hyperbaric chamber 1 is led
To 21 structure of guide post of ladder shaft type to form light-transmitting gap between cabin pedestal 2 and the bottom end of hyperbaric chamber 1;Limit is led
A limition orientation seat 23 is provided in limition orientation hole on plate 22;Limition orientation seat 23 is the cylindrical drum of a pair of of coaxial arrangement
Body structure, and a wherein cylindrical tube structure for limition orientation seat 23 is embedded in limition orientation hole, the limition orientation seat 23
Another cylindrical tube structure be pressed on the lower surface of limition orientation plate 22;The limition orientation column 21 is embedded in limition orientation seat
In 23.
Relief pipeline 3 is connected on the exhaust outlet of high pressure cabin, and relief valve 31 and pressure monitoring are provided on relief pipeline 3
Table 32.
Stabilization clamp holder 4 is arranged on cabin pedestal and is pressed on cabin pedestal 2 positioned at the bottom end surrounding of hyperbaric chamber 1
On guide post cascaded surface;Stabilization clamp holder 4 includes firm banking 41, hinged pedestal 42, pressing handle 43 and compact heap 44;It is fixed
Pedestal 41 is vertically set on cabin pedestal 2 and is distributed in the surrounding of hyperbaric chamber 1;Firm banking 41 is arranged in hinged pedestal 42
Top, pressing handle 43 and the orthogonal setting of compact heap 44 form L-type structure, and one end of pressing handle 43 is hinged on hinged base
On seat 42, it is pressed in compact heap 44 on limition orientation plate 22 by rotating pressing handle 43.
The working principle of the invention: cell tissue to be observed is mounted in being put into high pressure cabin 11 for hyperbaric chamber 1
Glass slide and culture solution;By top blind flange 12, top observation window 13, bottom cover plate 14 and bottom observation window 15, by high pressure
Cabin sealing;The hyperbaric chamber 1 for completing sealing is mounted on cabin pedestal 2 by guide post 21;And by cabin pedestal 2
Hyperbaric chamber 1 is pressed on cabin pedestal 2 by stabilization clamp holder 4;Then in whole placement and microscopy system stage;It opens high
Air inlet on ballasting body 11 is passed through gas and pressurizes, and detects the pressure inside hyperbaric chamber body by pressure monitoring table 32;
When reaching observed pressure, stopping is passed through gas and carries out observational record related data;After completing observation, it is logical to open relief valve 31
The gas in the exhaust outlet discharge high pressure cabin 11 on hyperbaric chamber body 11 is crossed, is opened in the case where being normal pressure in high pressure cabin 11
High pressure cabin 11 takes out internal glass slide, and tidies up dependent observation equipment, completes entire observation process.
It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, above-described embodiment and explanation
It is merely illustrated the principles of the invention described in book, without departing from the spirit and scope of the present invention, the present invention also has
Various changes and modifications, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention
It is defined by the appending claims and its equivalent thereof.
Claims (5)
1. a kind of cell hyperbaric chamber for being equipped on microscopic system, the cell hyperbaric chamber are equipped on the objective table of microscopic system
On, it is characterised in that: including hyperbaric chamber, cabin pedestal, relief pipeline and stabilization clamp holder;
The hyperbaric chamber includes high pressure cabin, top blind flange, top observation window, bottom cover plate and bottom observation window;The high pressure
Cabin is in bar shape structure and is equipped with the cylindrical chamber for accommodating culture solution and glass slide in hyperbaric chamber body, and the cylindrical chamber is by institute
State the conducting of high pressure cabin both ends;Air inlet and exhaust outlet are provided on the side of the high pressure cabin;The top blind flange and bottom
The top observation window and the bottom observation window are pressed on the both ends of high pressure cabin by portion's cover board respectively;
Several location holes are provided on the cabin pedestal, the cabin pedestal is locked at microscope by bolt by the location hole
Follow objective table mobile on the objective table of system;The guide post with the cooperation of hyperbaric chamber bottom end, institute are provided on the cabin pedestal
Stating guide post is stepped shaft structure;Edge in the bottom cover plate of the hyperbaric chamber is provided with limition orientation plate and limition orientation plate
On be provided with limition orientation hole, the limit of hyperbaric chamber is realized in the cascaded surface cooperation of the guide post on the limition orientation hole and cabin pedestal
Position guiding, the guide post structure of the ladder shaft type to form light-transmitting gap between cabin pedestal and the bottom end of hyperbaric chamber;
The relief pipeline is connected on the exhaust outlet of the high pressure cabin, is provided with relief valve and pressure on the relief pipeline
Monitor table;
The stabilization clamp holder is arranged on the cabin pedestal and is pressed on the cabin positioned at the bottom end surrounding of the hyperbaric chamber
On guide post cascaded surface on body pedestal.
2. a kind of cell hyperbaric chamber for being equipped on microscopic system according to claim 1, it is characterised in that: the stabilization
Clamper includes firm banking, hinged pedestal, pressing handle and compact heap;The firm banking is vertically set on cabin pedestal
And it is distributed in the surrounding of hyperbaric chamber;The top of firm banking is arranged in the hinged pedestal, and the pressing handle and compact heap are mutual
Perpendicular setting forms L-type structure, and one end of the pressing handle is hinged on hinged pedestal, makes to press by rotating pressing handle
Tight block is pressed on limition orientation plate.
3. a kind of cell hyperbaric chamber for being equipped on microscopic system according to claim 1, it is characterised in that: the limit
A limition orientation seat is provided in limition orientation hole on guide plate;The limition orientation seat is the cylindrical drum of a pair of of coaxial arrangement
Body structure, an and wherein cylindrical tube structure for limition orientation seat is embedded in limition orientation hole, the limition orientation seat it is another
One cylindrical tube structure is pressed on the lower surface of limition orientation plate;The limition orientation column is embedded in limition orientation seat.
4. a kind of cell hyperbaric chamber for being equipped on microscopic system according to claim 1, it is characterised in that: the top
Observation window and bottom observation window are the glass structure of blue coating film treatment.
5. a kind of cell hyperbaric chamber for being equipped on microscopic system according to claim 1, it is characterised in that: the top
Bolt hole is provided on the edge of cover board and the edge of bottom cover plate, top blind flange and bottom cover plate are locked by bolt respectively
On the bulkhead of high pressure cabin, the contact position of the end of the top blind flange and bottom cover plate and high pressure cabin is provided with O
Type sealing ring.
Priority Applications (1)
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CN201910813452.4A CN110530780A (en) | 2019-08-30 | 2019-08-30 | A kind of cell hyperbaric chamber being equipped on microscopic system |
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CN201910813452.4A CN110530780A (en) | 2019-08-30 | 2019-08-30 | A kind of cell hyperbaric chamber being equipped on microscopic system |
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Citations (9)
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US20050266547A1 (en) * | 2002-09-16 | 2005-12-01 | Wilhelm Scherze | Cell culture chamber for a cell culture system |
CN201495225U (en) * | 2009-08-13 | 2010-06-02 | 浙江大学 | Online microbe observation device |
US20140106444A1 (en) * | 2005-07-26 | 2014-04-17 | Corning Incorporated | Multilayered cell culture apparatus |
CN104181048A (en) * | 2014-08-14 | 2014-12-03 | 于荣国 | Gas pressure cell damage instrument |
CN204079984U (en) * | 2014-09-05 | 2015-01-07 | 中国科学技术大学 | A kind of microscope viable cell culture environment Controlling System |
US9023642B2 (en) * | 2006-07-07 | 2015-05-05 | The University Of Houston System | Method and apparatus for a miniature bioreactor system for long-term cell culture |
CN108611270A (en) * | 2018-05-08 | 2018-10-02 | 中国科学院力学研究所 | A kind of spatial cell biomechanics experiment system |
CN208844100U (en) * | 2018-06-27 | 2019-05-10 | 成都清科生物科技有限公司 | A kind of cell incubator that can monitor cell growth state |
-
2019
- 2019-08-30 CN CN201910813452.4A patent/CN110530780A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5998202A (en) * | 1997-02-26 | 1999-12-07 | Synthecon, Inc. | Multiple chamber diffusion vessel |
US20050266547A1 (en) * | 2002-09-16 | 2005-12-01 | Wilhelm Scherze | Cell culture chamber for a cell culture system |
US20140106444A1 (en) * | 2005-07-26 | 2014-04-17 | Corning Incorporated | Multilayered cell culture apparatus |
US9023642B2 (en) * | 2006-07-07 | 2015-05-05 | The University Of Houston System | Method and apparatus for a miniature bioreactor system for long-term cell culture |
CN201495225U (en) * | 2009-08-13 | 2010-06-02 | 浙江大学 | Online microbe observation device |
CN104181048A (en) * | 2014-08-14 | 2014-12-03 | 于荣国 | Gas pressure cell damage instrument |
CN204079984U (en) * | 2014-09-05 | 2015-01-07 | 中国科学技术大学 | A kind of microscope viable cell culture environment Controlling System |
CN108611270A (en) * | 2018-05-08 | 2018-10-02 | 中国科学院力学研究所 | A kind of spatial cell biomechanics experiment system |
CN208844100U (en) * | 2018-06-27 | 2019-05-10 | 成都清科生物科技有限公司 | A kind of cell incubator that can monitor cell growth state |
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Application publication date: 20191203 |