CN110495425A - A kind of conditionity is overexpressed the model production method of CD98 transgenic mice - Google Patents
A kind of conditionity is overexpressed the model production method of CD98 transgenic mice Download PDFInfo
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- 101000800023 Homo sapiens 4F2 cell-surface antigen heavy chain Proteins 0.000 title claims abstract description 35
- 101000605020 Homo sapiens Large neutral amino acids transporter small subunit 1 Proteins 0.000 title claims abstract description 34
- 102100033400 4F2 cell-surface antigen heavy chain Human genes 0.000 title claims abstract description 32
- 241000699660 Mus musculus Species 0.000 title claims abstract description 17
- 238000011830 transgenic mouse model Methods 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 11
- 241000699666 Mus <mouse, genus> Species 0.000 claims abstract description 40
- 108700019146 Transgenes Proteins 0.000 claims abstract description 15
- 238000000520 microinjection Methods 0.000 claims abstract description 12
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 12
- 101150044219 SLC3A2 gene Proteins 0.000 claims abstract description 8
- 230000002068 genetic effect Effects 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 108010051219 Cre recombinase Proteins 0.000 claims description 4
- 108010000912 Egg Proteins Proteins 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 4
- 210000004681 ovum Anatomy 0.000 claims description 4
- 238000011740 C57BL/6 mouse Methods 0.000 claims description 3
- 208000033040 Somatoform disorder pregnancy Diseases 0.000 claims description 3
- 238000010276 construction Methods 0.000 claims description 3
- 230000004720 fertilization Effects 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
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- 210000001015 abdomen Anatomy 0.000 claims 1
- 230000009261 transgenic effect Effects 0.000 abstract description 5
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- 230000005748 tumor development Effects 0.000 abstract description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 235000011449 Rosa Nutrition 0.000 description 2
- 239000003708 ampul Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 108020005345 3' Untranslated Regions Proteins 0.000 description 1
- 102100024630 Asc-type amino acid transporter 1 Human genes 0.000 description 1
- 102100035300 Cystine/glutamate transporter Human genes 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 101100110003 Danio rerio pycard gene Proteins 0.000 description 1
- 102100038204 Large neutral amino acids transporter small subunit 1 Human genes 0.000 description 1
- 102100038235 Large neutral amino acids transporter small subunit 2 Human genes 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 108091006313 SLC3A2 Proteins 0.000 description 1
- 108091006241 SLC7A11 Proteins 0.000 description 1
- 108091006232 SLC7A5 Proteins 0.000 description 1
- 108091006237 SLC7A6 Proteins 0.000 description 1
- 108091006236 SLC7A7 Proteins 0.000 description 1
- 108091006238 SLC7A8 Proteins 0.000 description 1
- 102100023935 Transmembrane glycoprotein NMB Human genes 0.000 description 1
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- 230000018109 developmental process Effects 0.000 description 1
- 239000000833 heterodimer Substances 0.000 description 1
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- 230000037431 insertion Effects 0.000 description 1
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- 230000004807 localization Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
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- 238000002360 preparation method Methods 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
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- 108091007466 transmembrane glycoproteins Proteins 0.000 description 1
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Abstract
The present invention relates to field of transgenic technology, more particularly to a kind of conditionity to be overexpressed the model production method of CD98 transgenic mice, the following steps are included: S1, CAG+LoxP+Stop+LoxP+Slc3a2 transgene carrier construct；S2, transgene carrier pronuclear microinjection；S3, strain identified for genes, it finally establishes in mouse genome and has the genetic mouse catastrophic model of external source CD98 gene, the present invention provides the model mice that conditionability induction is overexpressed CD98 albumen, the tool mouse mating of Cre is expressed by different parts, induction CD98 albumen is expressed in different tissues organ.The foundation of the model helps to study Different Organs tumor development mechanism, discovery drug novel targets and the preclinical drug effect of evaluation etc..
Description
Technical field
The present invention relates to the models that field of transgenic technology more particularly to a kind of conditionity are overexpressed CD98 transgenic mice
Production method.
Background technique
Transgenic mice is study of disease occurrence and development mechanism, the powerful for formulating therapeutic strategy.
CD98 isType transmembrane glycoprotein, about 85 kDa of size, by a heavy chain (CD98 heavy chain
[CD98hc], Slc3a2) and light chain (LAT1, LAT2, xCT, y+LAT1, y+LAT2 or asc1) covalently connected by disulfide bond
It connects to form heterodimer.CD98 is expected to become diagnosing tumor and judging prognosis it is verified that the high expression in kinds of tumors
New marker, and, proliferation, invasion including tumour cell, migration closely related with the biological behaviour of tumour cell
Deng being likely to become the new molecular target of oncotherapy.
Summary of the invention
Technical problem: the purpose of the present invention is to provide the modellings that a kind of conditionity is overexpressed CD98 transgenic mice
Method establishes CD98 conditionity by transgenic technology and is overexpressed mouse model, can stablize heredity, localization and expression to obtain one kind
The mouse model of CD98 albumen.The model will can be used for studying the albumen in body function analysis, disease incidence Mechanism Discussion, drug
The research such as novel targets discovery and preclinical evaluating drug effect, has highly important scientific meaning and clinical value.
To achieve the goals above, present invention employs following technical solutions:
A kind of conditionity is overexpressed the model production method of CD98 transgenic mice, comprising the following steps:
The building of S1, CAG+LoxP+Stop+LoxP+Slc3a2 transgene carrier；
S2, transgene carrier pronuclear microinjection；
S3, strain identified for genes are finally established in mouse genome and are had the genetic mouse catastrophic model of external source CD98 gene.
Preferably, the transgene carrier construction method of the first step is: CAG+LoxP+Stop+LoxP+Slc3a2 is expressed sequence
Column insertion mouse rosa 26 site.
Preferably, the transgene carrier pronuclear microinjection of second step is: the linear transgene carrier of external source is injected into
In C57BL/6 mouse fertilized egg nucleus；With the method for microinjection by the target gene fragment of linearisation be injected into mouse by
In the protokaryon of smart ovum, integrate it with mouse genome, as cell constantly divides, each cell will carry the segment；Finally
The CD98 transgenic mice of building can be overexpressed CD98 albumen under the action of Cre recombinase.
Preferably, the strain identified for genes of third step is: the fertilization implantation of ovum false pregnancy mouse that will be survived after microinjection
The ampulla of fallopian tubal generates F0 for transgenic mice, is integrated with exogenous expression's sequence in the mouse genome；The head of birth
Mouse for CD98 transgenic positive builds mouse headed by being；Every head mouse of founding a capital will be considered as an independent strain and breed,
Cut coda gene identification to every head mouse strain of founding a capital.
The beneficial effects of the present invention are: the present invention provides the model mice that conditionability induction is overexpressed CD98 albumen, lead to
The tool mouse mating of different parts expression Cre is crossed, induction CD98 albumen is expressed in different tissues organ.The foundation of the model, has
Help study Different Organs tumor development mechanism, discovery drug novel targets and the preclinical drug effect of evaluation etc..
Detailed description of the invention
Fig. 1 is that the CD98 for the model production method that a kind of conditionity proposed by the present invention is overexpressed CD98 transgenic mice turns
DNA murine vector construction policy map.
In figure: CAG is promoter, and SLC3A2 is sequence followed by 3'UTR and polyA sequence to be expressed, and polyA is eventually
Stop signal.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.
In the present embodiment, referring to Fig.1, a kind of conditionity is overexpressed the model production method of CD98 transgenic mice, including
Following steps:
The building of S1, CAG+LoxP+Stop+LoxP+Slc3a2 transgene carrier.Wherein, by CAG+LoxP+Stop+LoxP+
Slc3a2 expressed sequence is inserted into mouse rosa 26 site, as shown in Fig. 1.ROSA26 gene does not have any function, and sequence is inserted
Enter the expression of the site ROSA26 to stablize, and will not influence mouse phenotype.CAG is the strong promoter for capableing of whole body expression, LoxP sequence
It can be by Cre recombinase specific recognition.
S2, transgene carrier pronuclear microinjection.Wherein, the linear transgene carrier of external source is injected into C57BL/6 mouse
In fertilized egg cell's core；The target gene fragment of linearisation is injected into the protokaryon of mouse fertilized egg with the method for microinjection
In, integrate it with mouse genome, as cell constantly divides, each cell will carry the segment；The CD98 finally constructed
Transgenic mice can be overexpressed CD98 albumen under the action of Cre recombinase.
S3, strain identified for genes finally establish the genetic mouse in mouse genome with external source CD98 gene and are mutated
Model.Wherein, by the ampulla of the fallopian tubal of the fertilization implantation of ovum false pregnancy mouse survived after microinjection, F0 is generated for transgenosis
Mouse is integrated with exogenous expression's sequence in the mouse genome；Headed by the first mouse for CD98 transgenic positive of birth is
Build mouse；Every head mouse of founding a capital will be considered as an independent strain and breed, and carry out cutting tail base to every head mouse strain of founding a capital
Because of identification.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (4)
1. the model production method that a kind of conditionity is overexpressed CD98 transgenic mice, which comprises the following steps:
The building of S1, CAG+LoxP+Stop+LoxP+Slc3a2 transgene carrier；
S2, transgene carrier pronuclear microinjection；
S3, strain identified for genes are finally established in mouse genome and are had the genetic mouse catastrophic model of external source CD98 gene.
2. a kind of conditionity according to claim 1 is overexpressed the model production method of CD98 transgenic mice, feature
It is, the transgene carrier construction method of the first step is: CAG+LoxP+Stop+LoxP+Slc3a2 expressed sequence is inserted into mouse
The site ROSA26.
3. a kind of conditionity according to claim 1 is overexpressed the model production method of CD98 transgenic mice, feature
Be, the transgene carrier pronuclear microinjection of second step is: by the linear transgene carrier of external source be injected into C57BL/6 mouse by
In spermiovum core；The target gene fragment of linearisation is injected into the protokaryon of mouse fertilized egg with the method for microinjection,
Integrate it with mouse genome, as cell constantly divides, each cell will carry the segment；The CD98 finally constructed turns base
Because mouse can be overexpressed CD98 albumen under the action of Cre recombinase.
4. a kind of conditionity according to claim 1 is overexpressed the model production method of CD98 transgenic mice, feature
It is, the strain identified for genes of third step is: by the pot of the fallopian tubal of the fertilization implantation of ovum false pregnancy mouse survived after microinjection
Abdomen generates F0 for transgenic mice, is integrated with exogenous expression's sequence in the mouse genome；The head of birth turns base for CD98
Mouse is built headed by being because of positive mouse；Every head mouse of founding a capital will be considered as an independent strain and breed, and build to every head
Mouse strain all carries out cutting coda gene identification.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111019971A (en) * | 2019-12-19 | 2020-04-17 | 上海同科生物科技有限公司 | Construction method of mouse model for conditionally overexpressing HPV E6 gene at ROSA26 site |
| WO2023109956A1 (en) * | 2021-12-17 | 2023-06-22 | Biocytogen Jiangsu Co., Ltd. | Genetically modified non-human animal with human or chimeric cd98hc |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104278056A (en) * | 2013-07-09 | 2015-01-14 | 天津医科大学 | Preparation method of high-expression staphylococcal nuclease domain containing 1 (SND1)-FLAG transgenic mouse model |
| CN106282235A (en) * | 2015-05-19 | 2017-01-04 | 南华大学 | Slow virus carrier containing people's SLC3A2 gene and construction method thereof |
| CN106480099A (en) * | 2016-10-21 | 2017-03-08 | 赣南医学院第附属医院 | The H11 fixed point of conditionality overexpression Spp1 gene knocks in hybrid mice model and its construction method |
| CN106893740A (en) * | 2017-02-17 | 2017-06-27 | 南京医科大学 | A kind of model production method of conditionity overexpression AQP4 transgenic mices |
| CN108396036A (en) * | 2018-03-01 | 2018-08-14 | 昆明医科大学 | A kind of overexpression COX5A transgenic mouse models and its construction method and application |
| CN110132914A (en) * | 2019-04-28 | 2019-08-16 | 西南医科大学 | A kind of method of living body cardiac muscle piece optical imagery |
-
2019
- 2019-09-24 CN CN201910906211.4A patent/CN110495425A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104278056A (en) * | 2013-07-09 | 2015-01-14 | 天津医科大学 | Preparation method of high-expression staphylococcal nuclease domain containing 1 (SND1)-FLAG transgenic mouse model |
| CN106282235A (en) * | 2015-05-19 | 2017-01-04 | 南华大学 | Slow virus carrier containing people's SLC3A2 gene and construction method thereof |
| CN106480099A (en) * | 2016-10-21 | 2017-03-08 | 赣南医学院第附属医院 | The H11 fixed point of conditionality overexpression Spp1 gene knocks in hybrid mice model and its construction method |
| CN106893740A (en) * | 2017-02-17 | 2017-06-27 | 南京医科大学 | A kind of model production method of conditionity overexpression AQP4 transgenic mices |
| CN108396036A (en) * | 2018-03-01 | 2018-08-14 | 昆明医科大学 | A kind of overexpression COX5A transgenic mouse models and its construction method and application |
| CN110132914A (en) * | 2019-04-28 | 2019-08-16 | 西南医科大学 | A kind of method of living body cardiac muscle piece optical imagery |
Non-Patent Citations (1)
| Title |
|---|
| TAKAO SHISHIDO ET AL.: "TRANSFORMATION OF BALB3T3 CELLS CAUSED BY OVER-EXPRESSION OF", 《INTERNATIONAL JOURNAL OF CANCER》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111019971A (en) * | 2019-12-19 | 2020-04-17 | 上海同科生物科技有限公司 | Construction method of mouse model for conditionally overexpressing HPV E6 gene at ROSA26 site |
| WO2023109956A1 (en) * | 2021-12-17 | 2023-06-22 | Biocytogen Jiangsu Co., Ltd. | Genetically modified non-human animal with human or chimeric cd98hc |
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