CN110483658A - A kind of preparation method of hypoglycemic corn silk polysaccharide - Google Patents
A kind of preparation method of hypoglycemic corn silk polysaccharide Download PDFInfo
- Publication number
- CN110483658A CN110483658A CN201910939549.XA CN201910939549A CN110483658A CN 110483658 A CN110483658 A CN 110483658A CN 201910939549 A CN201910939549 A CN 201910939549A CN 110483658 A CN110483658 A CN 110483658A
- Authority
- CN
- China
- Prior art keywords
- extraction
- corn
- 90min
- corn stigma
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of preparation methods of hypoglycemic corn silk polysaccharide, which is characterized in that comprises the following processes: pretreatment corn stigma, and corn stigma is cleaned, is dried, 80-200 mesh is smashed it through and obtains corn stigma powder；Extraction, corn stigma powder is extracted, and the flooding time is 30-90min；Extraction temperature is 90-100 DEG C；Filtering, centrifugation, obtain corn stigma supernatant；Corn stigma supernatant rotary evaporation is concentrated into the 1/3 of original volume by alcohol precipitation, and dehydrated alcohol, 4 DEG C of stand at low temperature layerings, its sediment of centrifuging and taking are added afterwards；It is dry, sediment is freeze-dried to constant weight, Stigma Maydis crude polysaccharides are obtained；The present invention can improve the yield of corn silk polysaccharide while retaining corn silk polysaccharide active constituent.
Description
Technical field
The present invention relates to extraction process field, especially a kind of preparation method of hypoglycemic corn silk polysaccharide.
Background technique
Corn stigma is the style and column cap of gramineae plant corn, contains polysaccharide, alkaloids, flavonoids, saponins
Etc. a variety of chemical components.Chinese medicine thinks that corn stigma is sweet in flavor mild-natured, and energy inducing diuresis to remove edema expels the heat-evil, calming the liver and benefiting the lungs, treatment nephritic dropsy,
Hypertension, cholecystitis, diabetes etc. have been received by " Ministry of Health of the People's Republic of China's medicinal material standard " as a kind of medicinal herbs most in use
Record.
Clinically, corn stigma compounds the beautiful soup of manufactured ginseng, cooperation western medicine non-insulin-dependent sugar with other ingredients
Urine disease has obvious curative effects.Corn stigma fermentation preparation plays the role of the reduction blood glucose of highly significant to rabbit.Studies have shown that corn stigma
In water-soluble polysaccharide can reduce blood glucose in diabetic mice, promote hepatic glycogen synthesis, accelerate gluconeogenesis, and to diabetic mice
Metabolic organ's damage has repair.Therefore, the polysaccharide bioactive substance in development and utilization corn stigma has answers very well
With prospect and economic value.
After traditional corn silk polysaccharide production technology general step includes: corn stigma removal of impurities, drying, crushes, using solvent
Extraction method prepares corn silk polysaccharide, and extractant is usually ethyl alcohol, after product dried into obtain finished product, however use such technique not only
Time-consuming, and yield is relatively low.Market needs one kind while retaining corn silk polysaccharide active constituent, improves its yield
Preparation method.
Summary of the invention
To solve the deficiencies in the prior art, the purpose of the present invention is to provide a kind of preparations of hypoglycemic corn silk polysaccharide
Method can improve the yield of corn silk polysaccharide while retaining corn silk polysaccharide active constituent.
In order to achieve the above objectives, the present invention adopts the following technical scheme that:
A kind of preparation method of hypoglycemic corn silk polysaccharide, comprises the following processes:
Corn stigma is pre-processed,
Corn stigma is cleaned, is dried, 80-200 mesh is smashed it through and obtains corn stigma powder；
Extraction,
Corn stigma powder is extracted, the flooding time is 30-90min；Extraction temperature is 90-100 DEG C；Filtering, centrifugation,
Obtain corn stigma supernatant；
Alcohol precipitation,
Corn stigma supernatant rotary evaporation is concentrated into the 1/3 of original volume, dehydrated alcohol, 4 DEG C of stand at low temperature point are added afterwards
Layer, its sediment of centrifuging and taking；
It is dry,
Sediment is freeze-dried to constant weight, Stigma Maydis crude polysaccharides are obtained.
The preparation method of the hypoglycemic corn silk polysaccharide of one kind above-mentioned pre-processes corn stigma, and corn stigma is cleaned, is shone
It does, smash it through 170 meshes and obtain corn stigma powder.
The preparation method of the hypoglycemic corn silk polysaccharide of one kind above-mentioned, the mode of extraction are water extraction and alcohol precipitation method, flooding
Time is 90min；Extraction temperature is 95 DEG C.
The preparation method of the hypoglycemic corn silk polysaccharide of one kind above-mentioned, the mode of extraction are aided with micro- for water extraction and alcohol precipitation method
Wave；The flooding time is 30-90min；Extraction temperature is 90-100 DEG C；Microwave power is 100-300W.
The preparation method of the hypoglycemic corn silk polysaccharide of one kind above-mentioned, the mode of extraction are aided with super for water extraction and alcohol precipitation method
Sound；The flooding time is 30-90min；Extraction temperature is 90-100 DEG C；Ultrasonic temperature is 90-100 DEG C, ultrasonic time 30-
90min。
The preparation method of the hypoglycemic corn silk polysaccharide of one kind above-mentioned, the mode of extraction are aided with super for water extraction and alcohol precipitation method
Sound；The flooding time is 90min；Extraction temperature is 95 DEG C；Ultrasonic temperature is 95 DEG C, ultrasonic time 90min.
The preparation method of the hypoglycemic corn silk polysaccharide of one kind above-mentioned, process conditions are as follows: corn stigma smashes it through 170 mesh
Sieve sets extraction temperature as 95 DEG C under the leaching mode that water extraction and alcohol precipitation method is aided with ultrasound, extraction time 90min.
The invention has the beneficial effects that:
The present invention is using corn stigma as raw material, by using water extraction and alcohol precipitation method effectively to mention polysaccharide component after dries pulverizing
It takes, the active constituent of corn silk polysaccharide can be retained；
The present invention by establishing the mathematical model optimizing extracting parameter of quadratic regression equation, improve corn silk polysaccharide
Rate.
Detailed description of the invention
Fig. 1 is a kind of flow chart of embodiment of the invention；
Fig. 2 is the experimental result picture of present invention experiment one.
Specific embodiment
Specific introduce is made to the present invention below in conjunction with the drawings and specific embodiments.
As shown in Figure 1, a kind of preparation method of hypoglycemic corn silk polysaccharide, comprises the following processes:
Corn stigma is pre-processed,
Corn stigma is cleaned, is dried, 80-200 mesh is smashed it through and obtains corn stigma powder；
Extraction,
Corn stigma powder is extracted；Filtering, centrifugation, obtain corn stigma supernatant；
The mode of extraction includes: water extraction and alcohol precipitation method, and water extraction and alcohol precipitation method is aided with microwave, and water extraction and alcohol precipitation method is aided with ultrasound.
The mode of extraction is water extraction and alcohol precipitation method, and the flooding time is 30-90min；Extraction temperature is 90-100 DEG C.
The mode of extraction is that water extraction and alcohol precipitation method is aided with microwave；The flooding time is 30-90min；Extraction temperature is 90-100
℃；Microwave power is 100-300W.
The mode of extraction is that water extraction and alcohol precipitation method is aided with ultrasound；The flooding time is 30-90min；Extraction temperature is 90-100
℃；Ultrasonic temperature is 90-100 DEG C, ultrasonic time 30-90min.
Alcohol precipitation,
Corn stigma supernatant rotary evaporation is concentrated into the 1/3 of original volume, dehydrated alcohol, 4 DEG C of stand at low temperature point are added afterwards
Layer, its sediment of centrifuging and taking；
It is dry,
Sediment is freeze-dried to constant weight, Stigma Maydis crude polysaccharides are obtained.
Below by way of under experimental study difference mesh, different temperatures, soaking time, extracting mode, corn polysaccharide content
Changing rule.
Experiment one, influence of the different extracting modes to corn silk polysaccharide recovery rate, corn silk polysaccharide leaching mode is really
It is fixed:
Having investigated three kinds of different leaching modes, (water extraction and alcohol precipitation method, water extraction and alcohol precipitation method are aided with microwave, water extraction and alcohol precipitation method is aided with
Ultrasound) influence to corn silk polysaccharide recovery rate, corn silk polysaccharide is prepared with preparation method above；Three kinds of leaching modes use
The identical technological parameter in addition to leaching mode, i.e. 140 meshes；Extraction time 60min；Extracting temperature is 95 DEG C.
Supplementary explanation:
The condition of microwave: water extraction and alcohol precipitation method is aided with microwave: the flooding time is 30-90min；Extraction temperature is 90-100
℃；Microwave power is 100-300W；
The condition of ultrasound: water extraction and alcohol precipitation method is aided with ultrasound: the flooding time is 30-90min；Extraction temperature is 90-100
℃；Ultrasonic temperature is 90-100 DEG C, ultrasonic time 30-90min；Most suitable ultrasonic temperature is 95 DEG C, and optimal ultrasonic time is
90min。
Influence result of three kinds of different leaching modes to corn silk polysaccharide recovery rate is as shown in Figure 2.
Interpretation of result: the preferred water extraction and alcohol precipitation method of the leaching mode of corn stigma powder is aided with ultrasound, and ultrasonic temperature is 90-100 DEG C,
Ultrasonic time is 30-90min.
Experiment two, the determination of corn silk polysaccharide optimum extraction process parameter；
Ultrasound is aided with using water extraction and alcohol precipitation method, has investigated mesh mesh number, extraction time, extraction temperature and combinations thereof to corn
The influence of palpus polysaccharide extract rate.Response surface experiments factor level table is as shown in table 1, and Box-Behnken experimental design and result are such as
Shown in table 2, the secondary multinomial model variance analysis of regression equation is as shown in table 3.
1 response surface experiments factor level table of table
2 Box-Behnken design experiment scheme of table and its result
The secondary multinomial model variance analysis of 3 regression equation of table
Using corn silk polysaccharide recovery rate as evaluation index, the model of fit between recovery rate (Y) and extracting parameter is obtained are as follows:
Y=-1216.50914+26.02727X1-0.026908X2-0.00116528X3+0.00348333X1X2+
0.000991667X1X3-0.00005X2X3-0.13944X1 2-0.00214833X2 2-0.000267639X3 2
Wherein X1, X2, X3 are respectively temperature, time, mesh.
Determine that optimum extraction temperature is 95.85 DEG C by response surface model, the optimum extraction time is 91.41min, best mesh
Mesh number is 170.81 mesh, polysaccharide extract rate 14.69%.
For the accuracy of testing model prediction, using the optimum extraction condition of corn silk polysaccharide: corn stigma is smashed it through
170 meshes set extraction temperature as 95 DEG C, extraction time 90min under ultrasonic wave added, do three groups of parallel laboratory tests, obtain reality
Recovery rate is 14.94% ± 0.08%, and test value and predicted value are without significant difference.
To sum up, the optimum process condition that corn silk polysaccharide extracts is determined are as follows: corn stigma smashes it through 170 meshes, mentions in water
Alcohol deposition method is aided under the leaching mode of ultrasound, sets extraction temperature as 95 DEG C, extraction time 90min, repeated property is tested
Card, polysaccharide extract rate 14.94%.
The present invention is using corn stigma as raw material, by using water extraction and alcohol precipitation method effectively to mention polysaccharide component after dries pulverizing
It takes, the active constituent of corn silk polysaccharide can be retained；Again by establishing the mathematical model optimizing extracting parameter of quadratic regression equation,
Improve the yield of corn silk polysaccharide.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the above embodiments do not limit the invention in any form, all obtained by the way of equivalent substitution or equivalent transformation
Technical solution is fallen within the scope of protection of the present invention.
Claims (7)
1. a kind of preparation method of hypoglycemic corn silk polysaccharide, which is characterized in that comprise the following processes:
Corn stigma is pre-processed,
Corn stigma is cleaned, is dried, 80-200 mesh is smashed it through and obtains corn stigma powder；
Extraction,
Corn stigma powder is extracted, the flooding time is 30-90min；Extraction temperature is 90-100 DEG C；Filtering, centrifugation, obtain beautiful
Rice must supernatant；
Alcohol precipitation,
Corn stigma supernatant rotary evaporation is concentrated into the 1/3 of original volume, dehydrated alcohol is added afterwards, 4 DEG C of stand at low temperature are layered, from
The heart takes its sediment；
It is dry,
Sediment is freeze-dried to constant weight, Stigma Maydis crude polysaccharides are obtained.
2. the preparation method of the hypoglycemic corn silk polysaccharide of one kind according to claim 1, which is characterized in that pretreatment is beautiful
Rice must, corn stigma is cleaned, is dried, 170 meshes is smashed it through and obtains corn stigma powder.
3. the preparation method of the hypoglycemic corn silk polysaccharide of one kind according to claim 1, which is characterized in that the extraction
Mode be water extraction and alcohol precipitation method, the flooding time be 90min；Extraction temperature is 95 DEG C.
4. the preparation method of the hypoglycemic corn silk polysaccharide of one kind according to claim 1, which is characterized in that the extraction
Mode be water extraction and alcohol precipitation method be aided with microwave；The flooding time is 30-90min；Extraction temperature is 90-100 DEG C；Microwave power is
100-300W。
5. the preparation method of the hypoglycemic corn silk polysaccharide of one kind according to claim 1, which is characterized in that the extraction
Mode be water extraction and alcohol precipitation method be aided with ultrasound；The flooding time is 30-90min；Extraction temperature is 90-100 DEG C；Ultrasonic temperature is
90-100 DEG C, ultrasonic time 30-90min.
6. the preparation method of the hypoglycemic corn silk polysaccharide of one kind according to claim 5, which is characterized in that the extraction
Mode be water extraction and alcohol precipitation method be aided with ultrasound；The flooding time is 90min；Extraction temperature is 95 DEG C；Ultrasonic temperature is 95 DEG C, is surpassed
The sound time is 90min.
7. the preparation method of the hypoglycemic corn silk polysaccharide of one kind according to claim 1, which is characterized in that process conditions
Are as follows: corn stigma smashes it through 170 meshes, under the leaching mode that water extraction and alcohol precipitation method is aided with ultrasound, sets extraction temperature as 95 DEG C,
Extraction time is 90min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910939549.XA CN110483658A (en) | 2019-09-30 | 2019-09-30 | A kind of preparation method of hypoglycemic corn silk polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910939549.XA CN110483658A (en) | 2019-09-30 | 2019-09-30 | A kind of preparation method of hypoglycemic corn silk polysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110483658A true CN110483658A (en) | 2019-11-22 |
Family
ID=68544711
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910939549.XA Pending CN110483658A (en) | 2019-09-30 | 2019-09-30 | A kind of preparation method of hypoglycemic corn silk polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110483658A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114671960A (en) * | 2022-03-10 | 2022-06-28 | 吉林工商学院 | Preparation method of corn stigma polysaccharide and application of corn stigma polysaccharide in blood sugar reducing product |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101475649A (en) * | 2009-01-12 | 2009-07-08 | 夏晖 | Extracting process for coral fungi polysaccharide |
| CN102504039A (en) * | 2011-11-16 | 2012-06-20 | 安徽师范大学 | Extraction process of black corn silk polysaccharide |
| CN103396888A (en) * | 2013-08-09 | 2013-11-20 | 湖北中烟工业有限责任公司 | Preparation method of corn stigma extract for cigarettes |
| CN103750107A (en) * | 2014-01-25 | 2014-04-30 | 肖志勇 | Health-care product with blood glucose reducing function |
-
2019
- 2019-09-30 CN CN201910939549.XA patent/CN110483658A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101475649A (en) * | 2009-01-12 | 2009-07-08 | 夏晖 | Extracting process for coral fungi polysaccharide |
| CN102504039A (en) * | 2011-11-16 | 2012-06-20 | 安徽师范大学 | Extraction process of black corn silk polysaccharide |
| CN103396888A (en) * | 2013-08-09 | 2013-11-20 | 湖北中烟工业有限责任公司 | Preparation method of corn stigma extract for cigarettes |
| CN103750107A (en) * | 2014-01-25 | 2014-04-30 | 肖志勇 | Health-care product with blood glucose reducing function |
Non-Patent Citations (3)
| Title |
|---|
| 刘娟: "玉米须多糖提取及其含量测定方法", 《黑龙江医药科学》 * |
| 吴华等: "不同品种玉米须多糖含量的比较", 《农机化研究》 * |
| 王艺帷等: "响应曲面法优化玉米须多糖提取工艺的研究", 《云南化工》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114671960A (en) * | 2022-03-10 | 2022-06-28 | 吉林工商学院 | Preparation method of corn stigma polysaccharide and application of corn stigma polysaccharide in blood sugar reducing product |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111187366B (en) | Double-aqueous-phase extraction method of polygonatum sibiricum polysaccharide | |
| CN103142669A (en) | Enzyme method for extracting ginsenoside and obtained ginsenoside extractive | |
| CN102716367A (en) | Compound dendrobium candidum buccal tablet and preparation method of compound dendrobium candidum buccal tablet | |
| CN107027930A (en) | A kind of Rosa roxburghii honeysuckle bealth-care instant tea treasure and preparation method thereof | |
| CN105218695B (en) | A kind of lycium ruthenicum polysaccharide extract and preparation method thereof | |
| CN104987427A (en) | Complex enzyme microwave extraction method for optimizing lycium ruthenicum polysaccharide by utilizing response surface method | |
| CN111116323A (en) | Microwave-assisted subcritical technology for extracting cannabidiol and preparation method thereof | |
| CN110483658A (en) | A kind of preparation method of hypoglycemic corn silk polysaccharide | |
| CN101357192A (en) | Separation preparation method of gastrodia elata crude polysaccharide and gastrodia elata alcohol extract using fresh gastrodia elata as raw material | |
| CN105820934A (en) | Novel healthy liquor and making process thereof | |
| CN109134583A (en) | The method of triterpene compound is extracted from schefflera leaf using ionic liquid | |
| CN108358822A (en) | A method of continuously extracting a variety of active ingredients from matrimony vine cull fruit | |
| CN1175758C (en) | Method for separating and extracting cyclic nucleotide syrup, dietary fiber and jujube wax from jujube | |
| CN106990153A (en) | A kind of method for differentiating astragalus mongolicus and Astragalus membranacus | |
| CN101912436A (en) | Ultrasonic extraction method of alfalfa saponin | |
| CN110123946A (en) | A kind of process using saccharomyces cerevisiae solid state fermentation rhizoma polygonati | |
| CN105456277B (en) | Application of the ganodenic acid acid activity position in preparing blood fat reducing health products and drug | |
| CN108126000A (en) | Arasaponin extracts preparation method in fresh Radix Notoginseng | |
| CN103183740A (en) | Production method of fructus schisandrae polysaccharide | |
| CN105497413A (en) | Chinese yam overground part extract and preparing method and application thereof | |
| CN102942634B (en) | Chinese chive seed polysaccharide extract and extraction method thereof | |
| CN105646729A (en) | Method for safely and efficiently preparing polysaccharides of tricholoma matsutake | |
| CN105768090A (en) | Hericium erinaceus and fructus schisandrae chinensis syrup and preparation technology thereof | |
| CN112655964A (en) | Concentrated nutrient solution and preparation method thereof | |
| CN106619972B (en) | Capsule for reducing blood sugar containing hirsutella hepiali Chen et Shen mycelium powder, and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191122 |
|
| RJ01 | Rejection of invention patent application after publication |