CN110478382A - Extraction process, extract and the application of husky rice general flavone - Google Patents
Extraction process, extract and the application of husky rice general flavone Download PDFInfo
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- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The present invention provides extraction process, extract and the applications of a seed sand rice general flavone, are related to extracting flavonoids technology field.The extraction process includes the general flavone using ethanol solution ultrasound extraction Sha meter Zhong；The Extraction technique includes: 10~30min of ultrasonic time；Concentration of alcohol 50%~70%；Liquid-to-solid ratio (30~50): 1mL/g；40~60 DEG C of Extracting temperature；155~165W of ultrasonic power.The extraction process has extraction time short, the high advantage of flavones yield, and general flavone content is high in the extract extracted using the extraction process.
Description
Technical field
The present invention relates to extracting flavonoids technology field, more particularly, to a seed sand rice general flavone extraction process, mention
Take object and application.
Background technique
Husky rice belongs to the husky fluffy platymiscium of Chenopodiaceae, is annual herb, moving dunes pionner, be distributed widely in arid and
Semiarid zone is a kind of potential cereal crops, has high nutritive value and medical value.
As the edible wild plant of husky area's tradition, husky rice seed has at least long edible history in 1300.Husky rice seed
Grain is full of nutrition, containing about protein 23%, fatty acid 10%, carbohydrate 45%, can match in excellence or beauty with " space grain " quinoa.
Husky rice has the function such as potential anti-oxidant, antitumor, anti-inflammatory, antibacterial, lowering blood pressure and blood fat and protection cardiovascular and cerebrovascular
Energy.The dry aerial parts of husky rice plant can be used as medicine, and be traditional medicinal herbs most in use of Mongols's medicine.Chinese medicine thinks that husky rice is natural
Diet food, have aid digestion, strengthening the spleen and stomach effect, even more cardiovascular and cerebrovascular disease, renal function decline, diabetes patient ideal food
Product.Mongolian medicine thinks that Sha meter You dispels epidemic disease, heat-clearing, removing toxic substances, the function of diuresis.With its decoction made from powder agent or enter the scattered treatment pestilence of ball, headache is red
Mesh, jaundice, kidney heat, urethra cusalgia, stomach " He Yi ", aphthae, gums, which festers, waits diseases.Edible sand rice seed can reduce blood lipid, be good for
Taste promote digestion, improve microcirculation, strengthen immunity.
Flavones rich in husky rice, the medical value of flavones is very high, it can be with anticancer, anti-oxidant, antibacterium, disease
Poison and reducing blood pressure and blood fat are hypoglycemic etc., can be with many diseases of prophylactic treatment.Therefore flavones becomes the hot spot of recent researches.
Traditional extraction extracting flavonoids method, extraction time is long, needs a couple of days, and extraction efficiency is low；And the ancillary techniques such as enzyme auxiliary
Although shortening extraction time, it is not appropriate for being mass produced.Therefore, it is current for improving and extracting the extraction process of husky rice general flavone
It needs.
In view of this, the present invention is specifically proposed.
Summary of the invention
The first object of the present invention is to provide the extraction process of a seed sand rice general flavone, and the extraction process is from husky meter Zhong Ti
The general flavone content taken is high.
The second object of the present invention is to provide the extract being prepared using the extraction process of above-mentioned husky rice general flavone.
The third object of the present invention is that the extraction process for providing above-mentioned husky rice general flavone or said extracted object are preparing medicine
Application in object.
In order to solve the above technical problems, spy of the present invention adopts the following technical scheme that
According to an aspect of the present invention, the present invention provides the extraction processes of a seed sand rice general flavone, including use second
The general flavone of alcoholic solution ultrasound extraction Sha meter Zhong；Extraction technique includes: 10~30min of ultrasonic time；Concentration of alcohol 50%
~70%；Liquid-to-solid ratio (30~50): 1mL/g；40~60 DEG C of Extracting temperature；155~165W of ultrasonic power.
Preferably, the ultrasonic time is 10~20min, preferably 15min.
Preferably, the concentration of alcohol is 60~70%, preferably 70%.
Preferably, the liquid-to-solid ratio is (40~50): 1mL/g, preferably 50:1mL/g.
Preferably, the Extracting temperature is 40~50 DEG C, preferably 50 DEG C.
Preferably, the ultrasonic power is 160W.
Preferably, Extraction technique includes: ultrasonic time 15min；Concentration of alcohol 70%；Liquid-to-solid ratio 50:1mL/g；It mentions
Take temperature 50 C；Ultrasonic power: 160W.
Preferably, the aerial tissues extracting section sand rice general flavone of husky rice is selected；
Preferably, first that husky rice is dry to constant weight, it is used further to extract husky rice general flavone after crushing；
Preferably, using the content of the husky rice general flavone in plant flavonoids kit measurement extract.
According to another aspect of the present invention, the present invention also provides a kind of extraction processes using above-mentioned husky rice general flavone
The extract being prepared.
According to another aspect of the present invention, it the present invention also provides the extraction process of above-mentioned husky rice general flavone or above-mentioned mentions
Take the application of object in medicine preparation.
Compared with prior art, the invention has the following beneficial effects:
The extraction process of sand rice general flavone provided by the invention, using the flavones of ultrasonic wave extraction Sha meter Zhong, ultrasonic wave is mentioned
Flavones is taken to have extraction time short, the high advantage of flavones yield is suitble to industrialization large-scale production.Sand rice provided by the invention is total
The optimal extraction technology of flavones technological parameter, the extraction process by optimization can effectively reduce flavones resolution ratio, improve flavones
The quality of extraction.The extraction process is easy to operate, and process stabilizing, precision is relatively high, and low energy consumption, non-pollutant discharge, to ring
Border is friendly, is suitble to industrialization large-scale production, is a kind of economic, quick and efficient extraction process.It is mentioned using the extraction process
General flavone quality and content be all more preferably in the extract obtained.
The extraction process or said extracted object of sand rice general flavone provided by the invention can be applied to preparation treatment cardiovascular system
The drug for disease of uniting, and it is used to prepare the multiple functions such as antiviral and antitumor, resisting oxidation free radical, anti-inflammatory, analgesia and liver protection
Drug etc..
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
Applying example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.Unless otherwise indicated, profession used herein has the same meanings as commonly understood by one of ordinary skill in the art with scientific term.This
Outside, any method similar to or equal to what is recorded or material can also be applied in the present invention.
It should be noted that if the present invention is without particularly illustrating: all technical characteristics that the application mentions and excellent
Feature is selected to can be combined with each other to form new technical solution；Correspondingly, all embodiments and preferred embodiment can phases
Mutually combination forms new technical solution；Related each component or its preferred ingredient can be combined with each other to form new technical side
Case.If the present invention is without particularly illustrating: numberical range " a~b " indicates the contraction table of any real combinings between a to b
Show, wherein a and b is real number.
According to an aspect of the present invention, the present invention provides the extraction processes of a seed sand rice general flavone.The present invention uses
The general flavone of ethanol solution ultrasound extraction Sha meter Zhong.Ultrasonic wave extraction can substantially shorten extraction time, and flavones yield nor
Chang Gao；Ultrasonic extraction also has easy to operate, process stabilizing, the relatively high advantage of precision.
The optimal extraction technology of sand rice general flavone provided by the invention technological parameter, the extraction process by optimization can have
Effect reduces flavones resolution ratio, improves the quality of extracting flavonoids, and the technological parameter of the extraction process of present invention sand rice general flavone is as follows:
10~30min of ultrasonic time, such as can be but be not limited to 10min, 15min, 20min, 25min or 30min.
Concentration of alcohol 50%~70%, concentration of alcohol refer to the percent by volume in ethanol solution containing ethyl alcohol, such as
It can be but be not limited to 50%, 55%, 60%, 65% or 70%.
Liquid-to-solid ratio (30~50): 1mL/g, liquid-to-solid ratio refer to the volume ml of ethanol solution and the quality grams of husky rice
Than, such as can be but be not limited to 30:1mL/g, 35:1mL/g, 40:1mL/g, 45:1mL/g or 50:1mL/g.
40~60 DEG C of Extracting temperature, such as can be but be not limited to 40 DEG C, 45 DEG C, 50 DEG C, 55 DEG C or 60 DEG C.
155~165W of ultrasonic power, such as can be but be not limited to 155W, 160W or 165W.
The extraction process of sand rice general flavone provided by the invention has extraction time short, and the high advantage of flavones yield is suitble to
Industrialization large-scale production.The extraction process can be effectively reduced flavones resolution ratio, improve the quality of extracting flavonoids.The extraction work
Skill is easy to operate, and process stabilizing, precision is relatively high, and low energy consumption, and non-pollutant discharge is environmentally friendly, is suitble to industrialization big
Large-scale production is a kind of economic, quick and efficient extraction process.
It is always yellow that optimization ultrasonic time, concentration of alcohol, liquid-to-solid ratio, Extracting temperature and ultrasonic power can advanced optimize husky rice
The extraction process of ketone.Preferably, ultrasonic time is 10~20min, more preferably 15min；Preferably, concentration of alcohol is preferably 60
~70%, more preferably 70%；Preferably, liquid-to-solid ratio is preferably (40~50): 1mL/g, more preferably 50:1mL/g；Extract temperature
Preferably 40~50 DEG C, more preferably 50 DEG C of degree；Preferably, ultrasonic power 160W.
Optimize the cooperation between each parameter, the extraction effect of the extraction process of husky rice general flavone, In can be advanced optimized
In some preferred embodiments, Extraction technique includes: ultrasonic time 15min；Concentration of alcohol 70%；Liquid-to-solid ratio 50:
1mL/g；50 DEG C of Extracting temperature；Ultrasonic power: 160W.
In some preferred embodiments, the aerial tissues extracting section sand rice general flavone of husky rice is selected, more preferably first
Husky rice is dry to constant weight, it is used further to extract husky rice general flavone after crushing.
In some preferred embodiments, the total Huang extracted using plant flavonoids kits from Sha meter Zhong
Ketone content, the flavones content obtained using plant flavonoids kit measurement are more accurate.
According to another aspect of the present invention, the present invention also provides a kind of extraction processes using above-mentioned husky rice general flavone
The extract being prepared.Have the advantages that general flavone content is high using the extract that said extracted technique is prepared.
According to another aspect of the present invention, it the present invention also provides the extraction process of above-mentioned husky rice general flavone or above-mentioned mentions
Take the application of object in medicine preparation.The medical value of flavones is very high, has the function for the treatment of disease of cardiovascular system, also have
There are antibacterial and antiviral activity and antitumor, resisting oxidation free radical, anti-inflammatory, analgesia and liver protection function.Therefore above-mentioned husky rice
The extraction process or said extracted object of general flavone can be used for preparing a variety of drugs.
Technical solution of the present invention and beneficial effect are further illustrated below with reference to preferred embodiment.
Embodiment 1
The extracting method for present embodiments providing a seed sand rice general flavone includes the following steps: to dry husky rice aerial tissues
It does to constant weight, crushes, cross after 40 meshes, sand rice flour end is made.It weighs 1g sand rice flour to be added in conical flask, it is molten that ethyl alcohol is added
Liquid, carries out ultrasonic extraction in ultrasonic cleaning instrument, after ultrasonic extraction, takes after centrifugation (10000g is centrifuged 10min)
Layer clear liquid constant volume (with 60% ethanol solution constant volume into 100mL volumetric flask) is to get extracting solution.Extraction technique are as follows: super
The sound time is 10min；Concentration of alcohol is 60%；Liquid-to-solid ratio is 40:1mL/g；Extracting temperature is 50 DEG C；Ultrasonic power is 160W.
Embodiment 2
The extracting method of a seed sand rice general flavone is present embodiments provided, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min；Concentration of alcohol is 60%；Liquid-to-solid ratio is 40:1mL/g；
Extracting temperature is 50 DEG C；Ultrasonic power is 160W.
Embodiment 3
The extracting method of a seed sand rice general flavone is present embodiments provided, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 30min；Concentration of alcohol is 60%；Liquid-to-solid ratio is 40:1mL/g；
Extracting temperature is 50 DEG C；Ultrasonic power is 160W.
Embodiment 4
The extracting method of a seed sand rice general flavone is present embodiments provided, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min；Concentration of alcohol is 50%；Liquid-to-solid ratio is 30:1mL/g；
Extracting temperature is 50 DEG C；Ultrasonic power is 160W.
Embodiment 5
The extracting method of a seed sand rice general flavone is present embodiments provided, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min；Concentration of alcohol is 60%；Liquid-to-solid ratio is 30:1mL/g；
Extracting temperature is 50 DEG C；Ultrasonic power is 160W.
Embodiment 6
The extracting method of a seed sand rice general flavone is present embodiments provided, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min；Concentration of alcohol is 70%；Liquid-to-solid ratio is 30:1mL/g；
Extracting temperature is 50 DEG C；Ultrasonic power is 160W.
Embodiment 7
The extracting method of a seed sand rice general flavone is present embodiments provided, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min；Concentration of alcohol is 60%；Liquid-to-solid ratio is 50:1mL/g；
Extracting temperature is 50 DEG C；Ultrasonic power is 160W.
Embodiment 8
The extracting method of a seed sand rice general flavone is present embodiments provided, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min；Concentration of alcohol is 60%；Liquid-to-solid ratio is 40:1mL/g；
Extracting temperature is 40 DEG C；Ultrasonic power is 160W.
Embodiment 9
The extracting method of a seed sand rice general flavone is present embodiments provided, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min；Concentration of alcohol is 60%；Liquid-to-solid ratio is 40:1mL/g；
Extracting temperature is 60 DEG C；Ultrasonic power is 160W.
Embodiment 10
The extracting method of a seed sand rice general flavone is present embodiments provided, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 15min, concentration of alcohol 70%, and liquid-to-solid ratio 50:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 160W.
Comparative example 1
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 5min, concentration of alcohol 60%, liquid-to-solid ratio 40:1mL/g are mentioned
Taking temperature is 50 DEG C, ultrasonic power 160W.
Comparative example 2
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 40min, concentration of alcohol 60%, and liquid-to-solid ratio 40:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 160W.
Comparative example 3
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 50min, concentration of alcohol 60%, and liquid-to-solid ratio 40:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 160W.
Comparative example 4
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 40%, and liquid-to-solid ratio 30:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 160W.
Comparative example 5
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 80%, and liquid-to-solid ratio 30:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 160W.
Comparative example 6
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 60%, and liquid-to-solid ratio 10:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 160W.
Comparative example 7
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 60%, and liquid-to-solid ratio 20:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 160W.
Comparative example 8
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 60%, and liquid-to-solid ratio 60:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 160W.
Comparative example 9
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 60%, and liquid-to-solid ratio 40:1mL/g,
Extracting temperature is 20 DEG C, ultrasonic power 160W.
Comparative example 10
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 60%, and liquid-to-solid ratio 40:1mL/g,
Extracting temperature is 30 DEG C, ultrasonic power 160W.
Comparative example 11
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 60%, and liquid-to-solid ratio 40:1mL/g,
Extracting temperature is 70 DEG C, ultrasonic power 160W.
Comparative example 12
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 60%, and liquid-to-solid ratio 40:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 64W.
Comparative example 13
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 60%, and liquid-to-solid ratio 40:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 96W.
Comparative example 14
This comparative example provides the extracting method of a seed sand rice general flavone, the difference from embodiment 1 is that extraction process is joined
Number is different, the technological parameter of the present embodiment are as follows: ultrasonic time 20min, concentration of alcohol 60%, and liquid-to-solid ratio 40:1mL/g,
Extracting temperature is 50 DEG C, ultrasonic power 96W.
Effect example
With plant flavonoids kit measurement and calculate general flavone content: plant flavonoids kit is using the inscription life of Suzhou section
The plant flavonoids kit of object Technology Co., Ltd. production.According to kit operating procedure and Flavonoid Content calculation formula,
Determination step is as follows: taking 540 μ L extracting solutions, 30 μ L reagents 1 are added, mix well, 6min is stood at 25 DEG C, 30 μ L reagents are added
2, it mixes well, 25 DEG C stand lower 6min, and 400 μ L reagents 3 are added, mixes well, 15min is stood at 25 DEG C, and at 510nm
Measure light absorption value.
Standard curve: y=5.02x+0.0007, R2=0.9996；
Flavonoid Content (mg/g dry weight)=(△ A-0.0007) ÷ 5.02 × V sample ÷ (V sample ÷ V sample total × W)=19.2
(△A-0.0007)；
In above-mentioned formula: V sample is total: extracting liquid volume, 100ml is added；V sample: sample volume in reaction, 0.54ml；W: sample
Quality, 1g.△ A=A measurement-A blank.
It is as shown in table 1 that above-described embodiment 1~13 and above-mentioned comparative example 1~10 extract obtained general flavone content:
Table 1
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. the extraction process of a seed sand rice general flavone, which is characterized in that including using ethanol solution ultrasound to extract the total of Sha meter Zhong
Flavones；Extraction technique includes: 10~30min of ultrasonic time；Concentration of alcohol 50%~70%；Liquid-to-solid ratio (30~50):
1mL/g；40~60 DEG C of Extracting temperature；155~165W of ultrasonic power.
2. the extraction process of sand rice general flavone according to claim 1, which is characterized in that the ultrasonic time is 10~
20min, preferably 15min.
3. the extraction process of sand rice general flavone according to claim 1, which is characterized in that the concentration of alcohol is 60~
70%, preferably 70%.
4. the extraction process of sand rice general flavone according to claim 1, which is characterized in that the liquid-to-solid ratio be (40~
50): 1mL/g, preferably 50:1mL/g.
5. the extraction process of sand rice general flavone according to claim 1, which is characterized in that the Extracting temperature is 40~50
DEG C, preferably 50 DEG C.
6. the extraction process of sand rice general flavone according to claim 1, which is characterized in that the ultrasonic power is 160W.
7. the extraction process of sand rice general flavone according to claim 1-6, which is characterized in that Extraction technique
It include: ultrasonic time 15min；Concentration of alcohol 70%；Liquid-to-solid ratio 50:1mL/g；50 DEG C of Extracting temperature；Ultrasonic power: 160W.
8. the extraction process of sand rice general flavone according to claim 1-6, which is characterized in that select the ground of husky rice
Upper tissue part extracts husky rice general flavone；
Preferably, first that husky rice is dry to constant weight, it is used further to extract husky rice general flavone after crushing；
Preferably, using the content of the husky rice general flavone in plant flavonoids kit measurement extract.
9. the extract that the extraction process of the described in any item husky rice general flavones of claim 1-8 is prepared.
10. the extraction process of the described in any item husky rice general flavones of claim 1-8 or extract as claimed in claim 9 are being made
Application in standby drug.
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| CN201910930623.1A CN110478382A (en) | 2019-09-29 | 2019-09-29 | Extraction process, extract and the application of husky rice general flavone |
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| CN201910930623.1A CN110478382A (en) | 2019-09-29 | 2019-09-29 | Extraction process, extract and the application of husky rice general flavone |
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Citations (3)
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| CN103599148A (en) * | 2013-11-15 | 2014-02-26 | 沈阳药科大学 | Agriophyllum squarrosum total flavonoid extract, and preparation method and application thereof |
| WO2016156920A1 (en) * | 2015-03-29 | 2016-10-06 | Abrishamifar Seyyed Milad | Producing herbal medicine with mosquito stomach enzyme to treat virus and aids |
| CN108451992A (en) * | 2018-05-04 | 2018-08-28 | 包书茵 | Anaesthetic husky fluffy hypoglycemic active component and extraction process |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103599148A (en) * | 2013-11-15 | 2014-02-26 | 沈阳药科大学 | Agriophyllum squarrosum total flavonoid extract, and preparation method and application thereof |
| WO2016156920A1 (en) * | 2015-03-29 | 2016-10-06 | Abrishamifar Seyyed Milad | Producing herbal medicine with mosquito stomach enzyme to treat virus and aids |
| CN108451992A (en) * | 2018-05-04 | 2018-08-28 | 包书茵 | Anaesthetic husky fluffy hypoglycemic active component and extraction process |
Non-Patent Citations (2)
| Title |
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| 王雅等: "沙米乙醇提取物提取工艺及其体外抗氧化性能研究", 《中药材》 * |
| 陈建平等: "中蒙药黄酮的提取方法研究进展", 《内蒙古医学院学报》 * |
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