CN110477007A - A kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace - Google Patents
A kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace Download PDFInfo
- Publication number
- CN110477007A CN110477007A CN201910845162.8A CN201910845162A CN110477007A CN 110477007 A CN110477007 A CN 110477007A CN 201910845162 A CN201910845162 A CN 201910845162A CN 110477007 A CN110477007 A CN 110477007A
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- CN
- China
- Prior art keywords
- flowers
- hydrogen sulfide
- insect resistace
- sodium hydrosulfide
- new method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/24—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing heterocyclic radicals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
It is exactly in confined space using hydrogen sulfide (H the invention discloses a kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace2S, there is provided by NaHS liquid) sprinkling pretreatment is carried out to common flowers, the insect resistace that flowers can be enhanced well, significantly improve in plant leaf blade to the activity of pest-resistant relevant peroxidase (POD), polyphenol oxidase (PPO) and PAL enzyme (PAL).Reagent of the present invention is single, easy to operate, low in cost, provides a kind of completely new technical solution to solve to endanger pest problem for a long time in the industry of flowers and plants, is suitble to large-scale promotion application.
Description
Technical field
The present invention relates to a kind of new applications of known compound hydrogen sulfide, also belong to flowers insect resistace technical field, specifically
It is exactly a kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace for ground.
Background technique
With the needs of green city's construction and the raising of living standards of the people, flowers are enhancing health, purification air, are controlling
It treats disease and many aspects such as beautify the environment has played very important effect, flourishing for the industry of flowers and plants has also driven society
Meeting economic benefit substantially increases, and flowers demand increasingly improves.
Flowers during the growth process, are easy by Pests Damage, and prevention and treatment is difficult and prevention and control are improper will cause very big loss.It is existing
The method for having technology to mostly use spraying chemical insecticide or drug, hypertoxic high residue not only make pest be also easy to produce drug resistance, can also
Cause environmental pollution.Therefore study a kind of nonhazardous, noresidue, safety and environmental protection the method for raising flowers insect resistace just seem outstanding
It is important.
Hydrogen sulfide (H2S the third gas letter) being identified as after nitric oxide (NO) and carbon monoxide (CO)
Number.In medical research field, H2S has important physiological function in terms of regulating nervous system and cardiovascular system；In plant
Research field, H2It is a kind of important bioactive molecule that S, which is also identified as, participates in coordinate plant growth development, enhancing plant
Biological and abiotic resistance delays the various physiological processes such as plant senescence.At present about H2S grinds enhancing plant resistance to environment stress
Study carefully, mainly by using H2The donor of S handles vegetable material, observation and measurement plant physiology index variation, commonly uses H2S donor is sulphur
Sodium hydride (NaHS) and GYY4137, the two are dissolved in water and generate H2S, reaction mechanism are as follows: NaHS+H2O=Na++HS-+ H2O=H2S+
NaOH.Recently we have discovered that, H2S can also enhance flowers insect resistace.
Summary of the invention
The object of the present invention is to provide a kind of new methods with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace, pass through
The third gaseous signal molecule-H2S, which handles flowers, enhances flowers insect resistace, opens H2S newly answering in terms of plant biological resistance
With.
The H2The donor NaHS of S can be replaced with vulcanized sodium, calcium sulfide and GYY4137.
Compared with prior art, the present invention is a kind of new method of enhancing flowers insect resistace low in cost, easy to operate.
The present invention is as signaling molecule H2The donor NaHS of S, external source application concentration is very low, and optimum concentration is 40-100 μm of ol/L's
NaHS aqueous solution.The micro H released with NaHS aqueous solution2S handles flowers, can significantly increase flowers insect resistace, improves flower
In grass blade to the activity of pest-resistant relevant POD, PPO and PAL enzyme.
Detailed description of the invention
4 kinds of flowers insect resistace qualification results of Fig. 1；
Fig. 2 H2S processing is on Jasmine, chrysanthemum, Chinese rose, azalea peroxidase (POD) active influence；
Fig. 3 H2S processing is on Jasmine, chrysanthemum, Chinese rose, azalea polyphenol oxidase (PPO) active influence；
Fig. 4 H2S processing is on Jasmine, chrysanthemum, Chinese rose, azalea PAL enzyme enzyme (PAL) active influence.
Specific embodiment
Non-limiting examples of the invention are given below.
Embodiment 1
The consistent pretreated Jasmine of needs will be grown to put neatly, be placed into confined space.It 8 points or so in the morning, uses
The 80 μm of ol/L sodium hydrosulfide aqueous solutions now matched equably are sprayed on Jasmine blade, after 2 h of closed processes, by Jasmine
Confined space is removed, juxtaposition is at room temperature.It will cultivate in the lab, growth phase is consistent and is 5 arabian jasmine leafs of juvenile stage
It after wild 2 h of snout moth's larva Nature enemy, is inoculated on the Jasmine blade of processing with writing brush, the cylinder (top made of polyester transparency
Covered with gauze) isolation cover isolation after Routine Management.Control group is to spray distillation water process (CK1) and be inoculated with after spraying distilled water
Pest handles (CK2).In 0 h, 3 h, 6 h, 12 h, 24 h, 48 h, 72 h, 168 h collect blade storage for use, wherein often
20 repetitions of a material, 3 repetitions of each processing.The results showed that H2S processing can significantly reduce the breeding times of arabian jasmine leaf open country snout moth's larva
Rate and the activity for significantly improving the pest-resistant relevant enzyme of Jasmine (POD, PPO, PAL) improve Jasmine insect resistace.
Embodiment 2
The consistent pretreated chrysanthemum of needs will be grown to put neatly, be placed into confined space.8 points or so in the morning, with now
The 100 μm of ol/L sodium hydrosulfide aqueous solutions matched equably are sprayed on chrysanthemum leaf, after 4 h of closed processes, chrysanthemum are removed close
Space is closed, juxtaposition is at room temperature.It will cultivate in the lab, growth phase is consistent and is 5 aphid Nature enemies 2 of juvenile stage
It after h, is inoculated on the chrysanthemum leaf of processing with writing brush, is isolated with the cylinder (top is covered with gauze) that polyester transparency makes
Routine Management after cover isolation.Control group is that inoculation pest handles (CK2) after spraying distillation water process (CK1) and spraying distilled water.
In 0 h, 3 h, 6 h, 12 h, 24 h, 48 h, 72 h, 168 h collect blade storage for use, wherein 20 repetitions of each material,
3 repetitions of each processing.The results showed that H2S processing can significantly reduce aphid breeding multiplying power and to significantly improve chrysanthemum pest-resistant
The activity of relevant enzyme (POD, PPO, PAL) improves chrysanthemum insect resistace.
Embodiment 3
The consistent pretreated Chinese rose of needs will be grown to put neatly, be placed into confined space.8 points or so in the morning, with now
The 60 μm of ol/L sodium hydrosulfide aqueous solutions matched equably are sprayed on Chinese rose blade, after 6 h of closed processes, Chinese rose are removed close
Space is closed, juxtaposition is at room temperature.It will cultivate in the lab, at 5 consistent and for juvenile stage red spider starvation of growth phase
It after managing 2 h, is inoculated on the Chinese rose blade of processing with writing brush, the cylinder made of polyester transparency (top is covered with gauze)
Routine Management after isolation cover isolation.Control group is that inoculation pest is handled after spraying distillation water process (CK1) and spraying distilled water
(CK2).In 0 h, 3 h, 6 h, 12 h, 24 h, 48 h, 72 h, 168 h collect blade storage for use, wherein each material 20
A repetition, 3 repetitions of each processing.The experimental results showed that H2S processing can significantly reduce red spider breeding multiplying power and significantly improve
The activity of the pest-resistant relevant enzyme of Chinese rose (POD, PPO, PAL) improves Chinese rose insect resistace.
Embodiment 4
The consistent pretreated azalea of needs will be grown to put neatly, be placed into confined space.It 8 points or so in the morning, uses
The 40 μm of ol/L sodium hydrosulfide aqueous solutions now matched equably are sprayed on indian azalea leaf on piece, after 4 h of closed processes, by azalea
Confined space is removed, juxtaposition is at room temperature.It will cultivate in the lab, growth phase is consistent and is 5 azaleas of juvenile stage
After being preced with 2 h of lace bug Nature enemy, the indian azalea leaf on piece of processing is inoculated into writing brush, the cylinder (top made of polyester transparency
Portion is covered with gauze) isolation cover isolation after Routine Management.Control group is to spray distillation water process (CK1) and spray distilled water to be followed by
Kind pest processing (CK2).In 0 h, 3 h, 6 h, 12 h, 24 h, 48 h, 72 h, 168 h collection blade storage is stand-by, wherein
20 repetitions of each material, 3 repetitions of each processing.The results showed that H2It is numerous that S processing can significantly reduce cuckoo corolla lace bug
It grows multiplying power and significantly improves the activity of the pest-resistant relevant enzyme of azalea (POD, PPO, PAL), improve azalea insect resistace.
The invention is not only suitable for the processing of small lot flowers, is readily applicable to mass disposal flowers.It will first need to locate
The flowers of reason are put into closed space such as greenhouse, in greenhouse, handle flowers according still further to this illustration method, can reach identical technology
Effect.
Claims (4)
1. a kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace, the steps include:
The flowers seedling for choosing the proper growth stage is placed into confined space；
Configured certain density sodium hydrosulfide aqueous solution is equably sprayed on flower leaf on piece；
A period of time is placed in confined space, flowers is removed into confined space, juxtaposition is transplanted at room temperature.
2. a kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace as described in claim 1, uses flower
Grass growth phase is 45 day seedling stage.
3. a kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace as described in claim 1, sulphur hydrogen used
Change sodium preferred concentration is 40-100 μm of ol/L.
4. a kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace as described in claim 1, sprays sulphur hydrogen
After changing sodium water solution, preferably closed standing time is 2-6 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910845162.8A CN110477007A (en) | 2019-09-08 | 2019-09-08 | A kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910845162.8A CN110477007A (en) | 2019-09-08 | 2019-09-08 | A kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace |
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| CN110477007A true CN110477007A (en) | 2019-11-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910845162.8A Pending CN110477007A (en) | 2019-09-08 | 2019-09-08 | A kind of new method with hydrogen sulfide donor sodium hydrosulfide enhancing flowers insect resistace |
Country Status (1)
| Country | Link |
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| CN (1) | CN110477007A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114175930A (en) * | 2021-12-29 | 2022-03-15 | 南京农业大学 | Efficient rapid propagation method for accelerating growth of tea seedlings |
| CN114586615A (en) * | 2022-03-23 | 2022-06-07 | 西北农林科技大学 | Resistance induction method for watermelon seedling aphids |
-
2019
- 2019-09-08 CN CN201910845162.8A patent/CN110477007A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| HUA ZHANG 等: "Hydrogen sulfide acts as a regulator of flower senescence in plants", 《POSTHARVEST BIOLOGY AND TECHNOLOGY》 * |
| 史瑞 等: "10种杨树酶活性与抗性的关系", 《东北林业大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114175930A (en) * | 2021-12-29 | 2022-03-15 | 南京农业大学 | Efficient rapid propagation method for accelerating growth of tea seedlings |
| CN114586615A (en) * | 2022-03-23 | 2022-06-07 | 西北农林科技大学 | Resistance induction method for watermelon seedling aphids |
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Application publication date: 20191122 |
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