CN110467324A - A kind of method of novel photosynthetic bacteria solid matrix heavy metal biological toxicity in reducing printing and dyeing sludge - Google Patents
A kind of method of novel photosynthetic bacteria solid matrix heavy metal biological toxicity in reducing printing and dyeing sludge Download PDFInfo
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- CN110467324A CN110467324A CN201910456547.5A CN201910456547A CN110467324A CN 110467324 A CN110467324 A CN 110467324A CN 201910456547 A CN201910456547 A CN 201910456547A CN 110467324 A CN110467324 A CN 110467324A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/004—Sludge detoxification
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/30—Nature of the water, waste water, sewage or sludge to be treated from the textile industry
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- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
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- Health & Medical Sciences (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
Abstract
The present invention relates to microorganism application technologies and heavy metal control technology, and in particular to a kind of processing method of photosynthetic bacteria parasite matter bioavailability of heavy metals such as nickel, cadmium and lead in reducing printing and dyeing sludge.The present invention carries out parasitic culture to particulate biomass and photosynthetic bacteria, obtain a kind of novel photosynthetic bacteria solid matrix, a certain amount of water is sprayed in matrix, matrix is uniformly blended into printing and dyeing sludge again, pass through the biological effect of photosynthetic bacteria itself and the suction-operated of biomass, the oxidation-reduction potential of soil is improved, free state heavy metal is reduced, weakens heavy metal to the harmfulness of environment.The present invention, which applies, combines strain and biomass, weakens anaerobic environment to the toxic of strain, weakens the toxic of printing and dyeing sludge, improve the secondary use rate of sludge, avoid environmental pollution, is a kind of environmentally protective method.
Description
Technical field
The present invention relates to microorganism application technologies and heavy metal control technology, and in particular to a kind of photosynthetic bacteria parasite matter
The processing method of the bioavailability of heavy metals such as nickel, cadmium and lead in reducing printing and dyeing sludge.
Background technique
Printing and dyeing sludge is the secondary pollution of textile industry waste water, due to the consumption of the huge coloured cloth of China's scale of construction,
A large amount of printing and dyeing sludge is manipulated so for a significant problem.Since printing and dyeing sludge ingredient is sufficiently complex, typically contain dyestuff,
Surfactant, alkali material etc. wherein have the detection device for multi metallic elements such as PAH, nitro and copper, cadmium, zinc, arsenic, nickel in the composition of dyestuff,
And different enterprise's sludge compositions and characteristic difference are big, have the features such as bio-toxicity, environmental considerations are strong.Related heavy metal soil is dirty
The biological treatment research of dye, sludge has more document report { Li P Set al.Critical Reviews in
Microbiology, 2015,41 (2);Sfakianakis D G, et al.Environmental Research, 2015,
137;Farinati S, et al.Proteomics, 2009,9 (21);Li Yunshi etc., Acta Ecologica Sinica, 2015,35 (20);Hair
Avenge winged etc., Jiangsu's agriculture science, 2015,43 (5);Jiang Yumei etc., China Environmental Science, 2016,36 (11);Guo Wangmo etc., in
State's rice, 2007 (5);He Lili etc., Acta Ecologica Sinica, 2014,34 (15);Yu T, et al.Water Research, 2013,
47(1)}.By decades soil investigation, screens and has isolated many microorganism photosynthetic bacteria monoids that can convert heavy metal,
Strain is directly inoculated into dyeing waste water sludge, for photosynthetic bacteria monoid by toxic effects such as dyestuff, auxiliary agents, living environment is severe,
Its metabolism is exposed in sludge, by dyestuff, slurry, auxiliary agent murder by poisoning and the passivation of its heavy metal can not be played.It will
Photosynthetic bacteria is colonized in biomass and is added in the sludge environment containing bio-toxicity, and the existence of photosynthetic bacteria monoid can be improved
Ability, and the phase buffer that photosynthetic bacteria monoid adapts to printing and dyeing sludge environment is given, improve heavy metal in passivation printing and dyeing sludge
Efficiency.Photosynthetic bacteria combination biomass can not only weaken the heavy metal biological toxicity of printing and dyeing sludge, but also can effective conditioning of mud
In nutrient ratio.In the ascendant to the research of the biological treatment of printing and dyeing sludge at present, photosynthetic bacteria is combined the technology of biomass
Sludge after there is the heavy metal biological toxicity for weakening printing and dyeing sludge feasibility, heavy metal available state amount to reduce can be applied effectively
In Chinese garden planting industry.
Biomass is a kind of porous material that stalk obtains after anaeration in normal temperature bakes, and bakes preparation by medium temperature
The pore-size distribution of biological material is in 6.71~14.06nm.Biomass is the particulate matter containing aromatic ring molecular, can be parasitism
Photosynthetic bacteria good nutrient source and parasitic environment are provided.Biomass does not need to live using chemical method in the patent
Change, is directly mixed, it is easy to operate.
Summary of the invention
The technical problem to be solved in the invention: heavy metal biological toxicity is reduced for the bioanalysis of current printing and dyeing sludge
In, microorganism is by the toxic action of dyestuff, slurry, auxiliary agent, and the general oxidizing potential of microorganism in an anaerobic environment is low etc.,
Caused by bioanalysis poor processing effect the problem of, cause the problem that later period printing and dyeing sludge is strong in Chinese garden planting industry toxic, this
Invention, which provides a kind of photosynthetic bacteria combination biomass, reduces heavy metal biological toxic in printing and dyeing sludge.
To solve above-mentioned technical solution, the present invention, which adopts the following technical scheme that, is:
A kind of method of photosynthetic bacteria combination biomass heavy metal biological toxicity in reducing printing and dyeing sludge, concrete operation step is such as
Under:
(1) by weight, it is sprayed on 10 parts of photosynthetic bacterias and 2 parts of bacillus subtilis matrix with 8 parts of water, is put into 37 DEG C of cultures
Case.2 parts of moisturizing after 4 hours, every moisturizing in 3 hours 1 time later, 1 part of each moisturizing is sprayed 3 times altogether.Accelerate thallus activation, improves
Combination matrix is mixed into heavy metal transformation efficiency after printing and dyeing sludge.
(2) moisture content (40%~55%) after certain dehydration according to sludge, printing and dyeing dirt per ton are carried out in printing and dyeing sludge
Mud adds 150Kg~250Kg photosynthetic bacteria and is combined biomass matrix.
The preparation method of photosynthetic bacteria combination biomass matrix:
A. according to parts by weight, 60~70 parts of distilled water, 30~35 portions of beef extracts, 22~26 parts of sodium chloride, 16~19 parts of phosphorus are taken
Sour hydrogen dipotassium, 8~13 parts of potassium dihydrogen phosphates, 3~5 parts of magnesium chlorides, 0.2~0.4 part of N-methyl-N-nitrosourea stir evenly,
And sterilizing, obtain fluid nutrient medium;
B. 50 parts of complete rice straws are taken, 2 part of 3% sodium bicarbonate solution are uniformly sprayed, by the rice straw pressure after spray solution
Reality is simultaneously transferred in Muffle furnace, and 105 DEG C of preliminary drying 5min are arranged, and is warming up to 350 DEG C of 5~8min of desiccation.Controlling moisture content of straws is
It 5% or less and does not carbonize, biomass can be obtained after cooling.
C. 30~35 parts of sterile biomass after cooling are added in liquid medium.By the bacterium of inoculum concentration 3%~8%
Amount, photosynthetic bacteria is seeded in culture medium, and the culture medium after inoculation is placed in constant incubator, and set temperature is 27~37
DEG C, with 220rpm shaken cultivation base.Cultivate 12~27h;
D. after cultivating, the rice straw in culture medium is recycled, is dehydrated, that is, it is biomass-based to can be prepared into photosynthetic bacteria combination
Matter supplements 45% initial medium in remaining culture medium, continuously adds 30~35 parts of sterile biomass after cooling, circulation makes
With.
The group of photosynthetic bacteria becomes three kinds of Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria.
The present invention is compared with other methods, and beneficial effect is:
(1) strain that product of the invention is activated has excellent masking effect to the heavy metal in printing and dyeing sludge, effectively drops
The heavy metal biological toxicity of low printing and dyeing sludge.
(2) present invention application combines strain and biomass, and reduction anaerobic environment weakens printing and dyeing sludge to the toxic of strain
Toxic, improve the secondary use rate of sludge, and the method is environmentally protective, avoids environmental pollution.
Specific embodiment
The preparation method of photosynthetic bacteria combination biomass matrix:
A. according to parts by weight, 60~70 parts of distilled water, 30~35 portions of beef extracts, 22~26 parts of sodium chloride, 16~19 parts of phosphorus are taken
Sour hydrogen dipotassium, 8~13 parts of potassium dihydrogen phosphates, 3~5 parts of magnesium chlorides, 0.2~0.4 part of N-methyl-N-nitrosourea stir evenly,
And sterilizing, obtain fluid nutrient medium;
B. 50 parts of complete rice straws are taken, 2 part of 3% sodium bicarbonate solution are uniformly sprayed, by the rice straw pressure after spray solution
Reality is simultaneously transferred in Muffle furnace, and 105 DEG C of preliminary drying 5min are arranged, and is warming up to 350 DEG C of 5~8min of desiccation.Controlling moisture content of straws is
It 5% or less and does not carbonize, biomass can be obtained after cooling.
C. 30~35 parts of sterile biomass after cooling are added in liquid medium.By the bacterium of inoculum concentration 3%~8%
Amount, photosynthetic bacteria is seeded in culture medium, and the culture medium after inoculation is placed in constant incubator, and set temperature is 27~37
DEG C, with 220rpm shaken cultivation base.Cultivate 12~27h;
D. after cultivating, the rice straw in culture medium is recycled, is dehydrated, that is, it is biomass-based to can be prepared into photosynthetic bacteria combination
Matter supplements 45% initial medium in remaining culture medium, continuously adds 30~35 parts of sterile biomass after cooling, circulation makes
With.
Photosynthetic bacteria is mixed with purple sulfur bacteria, cyanobacterium, green sulfur bacteria with the ratio of 1:2:1.
A kind of method of photosynthetic bacteria combination biomass heavy metal biological toxicity in reducing printing and dyeing sludge, concrete operation step
It is as follows:
(1) by weight, it is sprayed on 10 parts of photosynthetic bacterias and 2 parts of bacillus subtilis matrix with 8 parts of water, is put into 37 DEG C of cultures
Case.2 parts of moisturizing after 4 hours, every moisturizing in 3 hours 1 time later, 1 part of each moisturizing is sprayed 3 times altogether.Accelerate thallus activation, improves
Combination matrix is mixed into heavy metal transformation efficiency after printing and dyeing sludge.
(2) moisture content (40%~55%) after certain dehydration according to sludge, printing and dyeing dirt per ton are carried out in printing and dyeing sludge
Mud adds 150Kg~250Kg photosynthetic bacteria and is combined biomass matrix.
Using the wastewater sludge of a certain printing and dyeing enterprise as research object (adjusting pH as 7.7) in experiment
Example 1
A kind of method of photosynthetic bacteria combination biomass heavy metal biological toxicity in reducing printing and dyeing sludge, concrete operation step is such as
Under:
(1) by weight, it is sprayed on 10 parts of photosynthetic bacterias and 2 parts of bacillus subtilis matrix with 8 parts of water, is put into 37 DEG C of cultures
Case.2 parts of moisturizing after 4 hours, every moisturizing in 3 hours 1 time later, 1 part of each moisturizing is sprayed 3 times altogether.Accelerate thallus activation, improves
Combination matrix is mixed into heavy metal transformation efficiency after printing and dyeing sludge.
(2) printing and dyeing enterprise dehydrated sludge (moisture content 53%) 5Kg is taken, applies the combination of 750g photosynthetic bacteria in printing and dyeing sludge
Biomass matrix, application, which finishes, to be placed in gnotobasis, at interval of 1 month detection Heavy Metals in Sludge bio-available Zn concentration, even
Continuous monitoring 3 months.
Example 2
A kind of method of photosynthetic bacteria combination biomass heavy metal biological toxicity in reducing printing and dyeing sludge, concrete operation step is such as
Under:
(1) by weight, it is sprayed on 10 parts of photosynthetic bacterias and 2 parts of bacillus subtilis matrix with 8 parts of water, is put into 37 DEG C of cultures
Case.2 parts of moisturizing after 4 hours, every moisturizing in 3 hours 1 time later, 1 part of each moisturizing is sprayed 3 times altogether.Accelerate thallus activation, improves
Combination matrix is mixed into heavy metal transformation efficiency after printing and dyeing sludge.
(2) printing and dyeing enterprise dehydrated sludge (moisture content 53%) 5Kg is taken, applies 1000g photosynthetic bacteria connection in printing and dyeing sludge
With biomass matrix, application, which finishes, to be placed in gnotobasis, at interval of 1 month detection Heavy Metals in Sludge bio-available Zn concentration,
Continuous monitoring 3 months.
Example 3
A kind of method of photosynthetic bacteria combination biomass heavy metal biological toxicity in reducing printing and dyeing sludge, concrete operation step is such as
Under:
(1) by weight, it is sprayed on 10 parts of photosynthetic bacterias and 2 parts of bacillus subtilis matrix with 8 parts of water, is put into 37 DEG C of cultures
Case.2 parts of moisturizing after 4 hours, every moisturizing in 3 hours 1 time later, 1 part of each moisturizing is sprayed 3 times altogether.Accelerate thallus activation, improves
Combination matrix is mixed into heavy metal transformation efficiency after printing and dyeing sludge.
(2) printing and dyeing enterprise dehydrated sludge (moisture content 53%) 5Kg is taken, applies 1250g photosynthetic bacteria connection in printing and dyeing sludge
With biomass matrix, application, which finishes, to be placed in gnotobasis, at interval of 1 month detection Heavy Metals in Sludge bio-available Zn concentration,
Continuous monitoring 3 months.
Control group: the printing and dyeing sludge of photosynthetic bacteria combination biomass matrix prepared by the present invention is not applied.
Case 4, case 5, case 6 are the photosynthetic bacterias and biomass applied respectively without activation processing, apply ratio
60:11, applied amount are every 5Kg printing and dyeing sludge 750g, 1000g, 1250g.
Analyze result such as table 1
Table 1
The present invention has the murder by poisoning sexuality for weakening printing and dyeing sludge as seen from the above table, has printing and dyeing sludge applied to agriculture forestry planting industry
There is feasibility.
Claims (5)
1. a kind of method of photosynthetic bacteria combination biomass heavy metal biological toxicity in reducing printing and dyeing sludge, is typically characterized by behaviour
Make step, as follows:
(1) by weight, it is sprayed on 10 parts of photosynthetic bacterias and 2 parts of bacillus subtilis matrix with 8 parts of water, is put into 37 DEG C of cultures
Case;2 parts of moisturizing after 4 hours, every moisturizing in 3 hours 1 time later, 1 part of each moisturizing is sprayed 3 times altogether;Accelerate thallus activation, improves
Combination matrix is mixed into heavy metal transformation efficiency after printing and dyeing sludge;
(2) moisture content (40% ~ 55%) after certain dehydration according to sludge, printing and dyeing sludge addition per ton are carried out in printing and dyeing sludge
150Kg ~ 250Kg photosynthetic bacteria is combined biomass matrix.
2. reducing the method for printing and dyeing sludge heavy metal biological toxicity as described in claim 1, it is characterised in that printed after dehydration per ton
It stains and applies addition 150Kg ~ 250Kg photosynthetic bacteria combination biomass matrix in mud.
3. reducing the method for printing and dyeing sludge heavy metal biological toxicity as described in claim 1 and 2, it is characterised in that photosynthetic bacteria combination
The preparation method of biomass matrix:
A. according to parts by weight, 60~70 parts of distilled water, 30~35 portions of beef extracts, 22~26 parts of sodium chloride, 16~19 parts of phosphorus are taken
Sour hydrogen dipotassium, 8~13 parts of potassium dihydrogen phosphates, 3~5 parts of magnesium chlorides, 0.2~0.4 part of N-methyl-N-nitrosourea stir evenly,
And sterilizing, obtain fluid nutrient medium;
B. 50 parts of complete rice straws are taken, 2 part of 3% sodium bicarbonate solution is uniformly sprayed, the rice straw after spray solution is compacted
And be transferred in Muffle furnace, 105 DEG C of preliminary drying 5min are set, 350 DEG C of 5 ~ 8min of desiccation are warming up to;Control moisture content of straws be 5% with
It does not carbonize down and, biomass can be obtained after cooling;
C. 30 ~ 35 parts of sterile biomass after cooling are added in liquid medium;It, will be photosynthetic by the bacterium amount of inoculum concentration 3% ~ 8%
Bacterium is seeded in culture medium, and the culture medium after inoculation is placed in constant incubator, and set temperature is 27 ~ 37 DEG C, with
220rpm shaken cultivation base;Cultivate 12 ~ 27h;
D. after cultivating, the rice straw in culture medium is recycled, is dehydrated, that is, it is biomass-based to can be prepared into photosynthetic bacteria combination
Matter supplements 45% initial medium in remaining culture medium, continuously adds 30 ~ 35 parts of sterile biomass after cooling, is recycled.
4. reducing the method for printing and dyeing sludge heavy metal biological toxicity as claimed in claim 3, it is characterised in that the preparation of biomass,
Need uniformly to spray a certain amount of 3% sodium bicarbonate solution on complete stalk before the biomass preparation.
5. reducing the method for printing and dyeing sludge heavy metal biological toxicity as claimed in claim 3, it is characterised in that the composition of photosynthetic bacteria
For three kinds of Mixed Microbes of purple sulfur bacteria, cyanobacterium and green sulfur bacteria.
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Application publication date: 20191119 |