CN110456046A - Immune rapid detection card - Google Patents
Immune rapid detection card Download PDFInfo
- Publication number
- CN110456046A CN110456046A CN201910788848.8A CN201910788848A CN110456046A CN 110456046 A CN110456046 A CN 110456046A CN 201910788848 A CN201910788848 A CN 201910788848A CN 110456046 A CN110456046 A CN 110456046A
- Authority
- CN
- China
- Prior art keywords
- rapid detection
- detection card
- reaction film
- positive electrode
- lower cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to the field of medical instrument technology, disclose a kind of immune rapid detection card, comprising: reaction film further includes the circuit structure for forming electric field on reaction film.Immune rapid detection card of the invention is equipped with the circuit structure that electric field is formed on reaction film, movement velocity and direction with electric field controls antigen-antibody reaction, so that it was thoroughly solved the problems, such as the uncontrollable of dereaction, greatly improves the accuracy of testing result, repeatability and sensitivity.
Description
Technical field
The present invention relates to the field of medical instrument technology, in particular to a kind of immune rapid detection card.
Background technique
Rapid detection method used in the quick analytical control field of clinical immunization includes: that gold marks, fluorescent marker is exempted from present
Epidemic disease chromatographic analysis rapid detection method etc..Above two method all be use the measured object on reaction film (antigen or antibody) with
The uncontrollable reaction pattern of marker (antibody or antigenic label) free movement chromatography.Due to the inhomogeneity of reaction film, no
Different from quality with measured object molecule (antigen or antibody) structure, the factors such as different measured object interfering substances containing different motion are led
Antigen-antibody reaction movement velocity and the uncontrollable problem in direction are caused, so that the poor repeatability of inspection result, inspection result
Poor accuracy examines sensitivity low, hinders the development and extensive utilization examined in clinical especially clinical emergency.
Summary of the invention
The present invention proposes a kind of immune detection card, and antigen-antibody reaction movement velocity and direction can not in the prior art for solution
The problem of control.
A kind of immune rapid detection card of the invention, comprising: reaction film further includes for forming electric field on reaction film
Circuit structure.
Wherein, further includes: lower cover and upper cover, the circuit structure include: positive electrode and negative electrode, and the lower lid is equipped with
Reaction film slot, the reaction film are placed in the reaction film slot, and the positive electrode and negative electrode are electrically connected the reaction film
Both ends, the upper cover and the lower cover lid, which close, to be arranged, and the region of corresponding reaction die cavity is respectively equipped with dripping hole on the upper lid
And observation window.
Wherein, the circuit structure further include: constant current constant voltage circuit board, the constant current constant voltage circuit board connect the positive electricity
Pole.
Wherein, the positive electrode and negative electrode are arranged on lower lid, and upper cover corresponds to the area of the positive electrode and negative electrode
Domain is respectively equipped with via hole.
Wherein, the positive electrode and negative electrode are respectively positioned on the same end of the lower cover.
Wherein, the upper lid is equipped with identification code paste area.
Wherein, the region corresponded on reaction film slot between dripping hole and observation window is equipped with pressing plate.
Wherein, the lower cover and upper cover are respectively equipped with unfilled corner, and respective unfilled corner covers conjunction relatively after lower cover and the conjunction of upper cover lid.
The circuit structure measured object that immune rapid detection card of the invention is equipped with the formation electric field on reaction film is instilled into instead
After answering on film, the small molecules such as moisture and salinity inside measured object can quickly be infiltrated from reaction film one end to another due to capillary phenomenon
One end, the movement velocity of electric field controls antigen-antibody reaction and direction make it thoroughly solve the speed of antigen-antibody reaction campaign in the past
Degree and the uncontrollable problem in direction greatly improve the accuracy of testing result, repeatability and sensitivity, realize quickly and effectively to quilt
Detectable substance carries out quantitative analysis.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention without any creative labor, may be used also for those of ordinary skill in the art
To obtain other drawings based on these drawings.
Fig. 1 is a kind of immune rapid detection card (lower cover) structural schematic diagram of the invention；
Fig. 2 is a kind of immune rapid detection card (upper cover) structural schematic diagram of the invention；
Fig. 3 is the immune rapid detection card schematic diagram of the lower cover of Fig. 1 and the upper cover Gai Hehou of Fig. 2.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
The immune rapid detection card of the present embodiment includes: reaction film, further includes the electricity for forming electric field on reaction film
Line structure.The circuit structure is used to form electric field at reaction film both ends, that is, passes through the antigen-antibody corresponding in antigen-antibody
Electric field is formed on the reaction film of marker to control antigen-antibody movement velocity and direction, so that the movement speed of antigen-antibody reaction
Degree is controllable with direction, to greatly improve the accuracy of testing result, repeatability and sensitivity are realized quickly and effectively to tested
It surveys object and carries out quantitative analysis.
As illustrated in fig. 1 and 2, the immune rapid detection card of the present embodiment specifically includes: lower cover 1, upper cover 2, reaction film 3, just
Electrode 4 and negative electrode 5, lower cover 1 are equipped with reaction film slot 6, and reaction film 3 is placed in reaction film slot 6, and positive electrode 4 and negative electrode 5 divide
Not electricity Lian Jie the both ends of reaction film 3 form electric field for being powered between the both ends of reaction film 3.Upper cover 2 and 1 Gai Heshe of lower cover
It sets, the region of corresponding reaction die cavity 6 is respectively equipped with dripping hole 7 and observation window 8 in upper cover 2, and dripping hole 7 is for dripping to measured object
On reaction film 3, by observation window 8 it can be observed that reaction result, observation window 8 can be (or multiple) strip-shaped hole.
3 both ends of reaction film are connect with positive and negative electrode by the immune rapid detection card of the present embodiment respectively, and measured object is instilled into
After on reaction film 3, the small molecules such as moisture and salinity inside measured object can quickly be infiltrated from 3 one end of reaction film due to capillary phenomenon
To the other end, after positive and negative electrode is passed through controllably stable electric current, the movement velocity of electric field controls antigen-antibody reaction and direction make
It thoroughly solved the problems, such as the uncontrollable of dereaction.
In the present embodiment, circuit structure further include: constant current constant voltage circuit board 9, constant current constant voltage circuit board 9 connect positive electrode 4,
For the voltage and electric current in stable detection card, fixation and the mounting structure of positive electrode 4 can also be used as.The constant current constant voltage circuit
Plate 9 is PCB circuit board, above the devices such as integrated resistor capacitor.
In the present embodiment, positive electrode 4 and negative electrode 5 are arranged on lower cover 1, and the corresponding positive electrode 4 of upper cover 2 and negative electrode 5
Region be respectively equipped with via hole, i.e. positive electrode via hole 10 and negative electrode via hole 11, make positive electrode 4 and negative electrode 5 respectively by just
Electrode via hole 10 and negative electrode via hole 11 expose upper cover 2, to contact respectively with the positive negative contact of power supply.4 He of certain positive electrode
As long as the setting of the respective position and structure of negative electrode 5, which meets, can distinguish the two of haptoreaction film after upper cover 2 and the lid of lower cover 1 are closed
End, and respective contact is exposed at upper cover 2 or lower cover 1 is outer.Further, positive electrode 4 and negative electrode 5 are respectively positioned on the same of lower cover
One end, convenient for the setting of positive electrode 4, negative electrode 5 and constant current constant voltage circuit board 9, and convenient for one end insertion confession of the detection card
Electrical slot.
Upper cover 2 is equipped with identification code paste area 12, for putting up the two dimensional code (or bar code) for being stored with patient information, detects
After the completion by scanning the two-dimensional code (or bar code) quick obtaining patient information, to will test result and patient's binding.
The region corresponded between dripping hole 7 and observation window 8 on reaction film slot 6 is equipped with pressing plate 13, for pushing down reaction film 3,
It is firmly secured at it in reaction film slot 6.
Lower cover 1 and upper cover 2 are respectively equipped with unfilled corner, and respective unfilled corner covers conjunction, unfilled corner relatively after lower cover 1 and the lid conjunction of upper cover 2
Rapid-aligning when being closed for covering, and when will test card insertion and enter for electrical slot, the direction of unfilled corner can be observed to determine whether
It is correctly inserted into (i.e. positive electrode 4 and the respective contact of negative electrode 5 is contacted with the positive negative contact of power supply respectively).
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle (its fortune is controlled by adding electrode on the reaction film of the corresponding antigen-antibody marker of antigen-antibody
The scheme of dynamic speed and direction), any modification, equivalent replacement, improvement and so on should be included in protection scope of the present invention
Within.
Claims (8)
1. a kind of immune rapid detection card, comprising: reaction film, which is characterized in that further include for forming electric field on reaction film
Circuit structure.
2. immune rapid detection card as described in claim 1, which is characterized in that further include: lower cover and upper cover, the circuit knot
Structure includes: positive electrode and negative electrode, and the lower lid is equipped with reaction film slot, and the reaction film is placed in the reaction film slot, institute
State positive electrode and negative electrode be electrically connected the both ends of the reaction film, the upper cover and the lower cover lid, which close, to be arranged, it is described on
The region for covering corresponding reaction die cavity is respectively equipped with dripping hole and observation window.
3. immune rapid detection card as claimed in claim 2, which is characterized in that the circuit structure further include: constant current constant voltage
Circuit board, the constant current constant voltage circuit board connect the positive electrode.
4. immune rapid detection card as claimed in claim 2, which is characterized in that the positive electrode and negative electrode are arranged in lower cover
On, and upper cover corresponds to the positive electrode and the region of negative electrode is respectively equipped with via hole.
5. immune rapid detection card as claimed in claim 4, which is characterized in that the positive electrode and negative electrode are respectively positioned on described
The same end of lower cover.
6. immune rapid detection card as claimed in claim 2, which is characterized in that the upper lid is equipped with identification code paste area.
7. immune rapid detection card as claimed in claim 2, which is characterized in that correspond to dripping hole and observation window on reaction film slot
Between region be equipped with pressing plate.
8. immune rapid detection card as claimed in claim 2, which is characterized in that the lower cover and upper cover are respectively equipped with unfilled corner,
And respective unfilled corner covers conjunction relatively after lower cover and the conjunction of upper cover lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910788848.8A CN110456046A (en) | 2019-08-26 | 2019-08-26 | Immune rapid detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910788848.8A CN110456046A (en) | 2019-08-26 | 2019-08-26 | Immune rapid detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110456046A true CN110456046A (en) | 2019-11-15 |
Family
ID=68488984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910788848.8A Pending CN110456046A (en) | 2019-08-26 | 2019-08-26 | Immune rapid detection card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110456046A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU8242198A (en) * | 1997-07-22 | 1999-02-16 | Arkray, Inc. | Densitometer, test piece for the densitometer, biosensor system, and method for forming terminal of the test piece |
| JP2003262601A (en) * | 2002-03-07 | 2003-09-19 | Matsushita Electric Ind Co Ltd | Immunological electrode sensor |
| CN101074963A (en) * | 2006-05-17 | 2007-11-21 | 聿新生物科技股份有限公司 | Electrode testing strip for inspecting cholesterine by electrochemical method and its production |
| CN101509924A (en) * | 2009-03-30 | 2009-08-19 | 湖南省宜生科技有限公司 | Electrochemical sidestream immune quantitative test paper sensor based on microgap array electrode and method thereof for detecting biotoxin |
| CN105675460A (en) * | 2016-03-08 | 2016-06-15 | 重庆理工大学 | Method for accelerating blood sedimentation by virtue of voltage |
| CN106324064A (en) * | 2015-06-23 | 2017-01-11 | 中国科学院上海应用物理研究所 | Electrochemical bio-sensing system |
| CN206684100U (en) * | 2017-03-17 | 2017-11-28 | 王志伟 | A kind of electrophoretic apparatus and electrophoresis system |
-
2019
- 2019-08-26 CN CN201910788848.8A patent/CN110456046A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU8242198A (en) * | 1997-07-22 | 1999-02-16 | Arkray, Inc. | Densitometer, test piece for the densitometer, biosensor system, and method for forming terminal of the test piece |
| JP2003262601A (en) * | 2002-03-07 | 2003-09-19 | Matsushita Electric Ind Co Ltd | Immunological electrode sensor |
| CN101074963A (en) * | 2006-05-17 | 2007-11-21 | 聿新生物科技股份有限公司 | Electrode testing strip for inspecting cholesterine by electrochemical method and its production |
| CN101509924A (en) * | 2009-03-30 | 2009-08-19 | 湖南省宜生科技有限公司 | Electrochemical sidestream immune quantitative test paper sensor based on microgap array electrode and method thereof for detecting biotoxin |
| CN106324064A (en) * | 2015-06-23 | 2017-01-11 | 中国科学院上海应用物理研究所 | Electrochemical bio-sensing system |
| CN105675460A (en) * | 2016-03-08 | 2016-06-15 | 重庆理工大学 | Method for accelerating blood sedimentation by virtue of voltage |
| CN206684100U (en) * | 2017-03-17 | 2017-11-28 | 王志伟 | A kind of electrophoretic apparatus and electrophoresis system |
Non-Patent Citations (1)
| Title |
|---|
| 郭鑫: "《动物免疫学实验教程》", 28 February 2017, 中国农业大学出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105556307B (en) | The bioassay of electrochemistry lateral flow and biosensor | |
| JP4027596B2 (en) | Device for determining blood clotting by capacitance or resistance | |
| JP7339430B2 (en) | Lateral fluid fluid testing system and method | |
| Yoshimura et al. | Physical properties of the probe electrospray ionization (PESI) needle applied to the biological samples | |
| CN109061190B (en) | Preparation of multi-channel biosensor array based on paper chip and application of multi-channel biosensor array in immunodetection | |
| Zhao et al. | The review of Lab‐on‐PCB for biomedical application | |
| CN102089079A (en) | Apparatus and methods for effecting chemical assays | |
| CN110192109A (en) | The combined immunization measurement of sensitivity for spreading range and magnetic immunoassay system and device | |
| Ansari et al. | A portable lab‐on‐a‐chip instrument based on MCE with dual top–bottom capacitive coupled contactless conductivity detector in replaceable cell cartridge | |
| CN102089661B (en) | Sample carrier for effecting chemical assays | |
| CN105181780B (en) | Based on the metronidazole electrochemical sensor and preparation method of novel print identification and application | |
| JP2014531027A (en) | Electrochemical analysis | |
| CN110214274A (en) | Use the immunoassay apparatus of the spreading range of immunosensor and magnetic immunosensor | |
| CN104870999B (en) | Sensor integrated and its application in sidestream immune is analyzed | |
| CN105004781A (en) | Dopamine detecting method based on paper-base electrochemistry device | |
| CN108918865A (en) | Fluorescence immune chromatography test paper bar and reagent card | |
| CN215297225U (en) | Novel electrochemical immunodetection system for coronavirus 2019-nCoV | |
| CN101019764A (en) | Pyloric helicobacter detecting method | |
| CN110456046A (en) | Immune rapid detection card | |
| Tsai et al. | Battery‐powered portable instrument system for single‐cell trapping, impedance measurements, and modeling analyses | |
| CN207198082U (en) | A kind of micro-nano biological detection chip of variable capacitance | |
| KR20090062591A (en) | Sensing apparatus and sensing method using dielectrophoretic impedance | |
| KR20160102232A (en) | Hand-held test meter with an operating range test strip simulation circuit block | |
| CN208999349U (en) | A kind of test paper detecting blood glucose | |
| CN204269656U (en) | A kind of test macro and immuno-chromatographic test paper strip thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191115 |
|
| RJ01 | Rejection of invention patent application after publication |