CN110453293A - A method of fibrinogen is extracted using enzymatic isolation method - Google Patents
A method of fibrinogen is extracted using enzymatic isolation method Download PDFInfo
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- CN110453293A CN110453293A CN201910928352.6A CN201910928352A CN110453293A CN 110453293 A CN110453293 A CN 110453293A CN 201910928352 A CN201910928352 A CN 201910928352A CN 110453293 A CN110453293 A CN 110453293A
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- fibrinogen
- enzyme
- oasis
- clear water
- digestion reaction
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- 238000000034 method Methods 0.000 title claims abstract description 43
- 102000008946 Fibrinogen Human genes 0.000 title claims abstract description 36
- 108010049003 Fibrinogen Proteins 0.000 title claims abstract description 36
- 229940012952 fibrinogen Drugs 0.000 title claims abstract description 36
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 6
- 238000002955 isolation Methods 0.000 title claims abstract description 5
- 238000006243 chemical reaction Methods 0.000 claims abstract description 41
- 238000001976 enzyme digestion Methods 0.000 claims abstract description 34
- 108090000790 Enzymes Proteins 0.000 claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 claims abstract description 32
- 239000004459 forage Substances 0.000 claims abstract description 26
- 239000002699 waste material Substances 0.000 claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 9
- 239000000835 fiber Substances 0.000 claims description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 238000001914 filtration Methods 0.000 claims description 20
- 238000009835 boiling Methods 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 18
- 238000007670 refining Methods 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 14
- 239000004902 Softening Agent Substances 0.000 claims description 13
- 238000004064 recycling Methods 0.000 claims description 13
- 238000004140 cleaning Methods 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 10
- 230000009514 concussion Effects 0.000 claims description 7
- 108010059820 Polygalacturonase Proteins 0.000 claims description 6
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 6
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 6
- 238000009960 carding Methods 0.000 claims description 5
- 230000035484 reaction time Effects 0.000 claims description 5
- 238000005096 rolling process Methods 0.000 claims description 5
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 8
- 238000009991 scouring Methods 0.000 abstract description 7
- 238000002360 preparation method Methods 0.000 abstract description 4
- 229920005610 lignin Polymers 0.000 abstract description 3
- 229920002678 cellulose Polymers 0.000 abstract description 2
- 239000001913 cellulose Substances 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 229920002488 Hemicellulose Polymers 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000004753 textile Substances 0.000 abstract 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 12
- 235000017491 Bambusa tulda Nutrition 0.000 description 12
- 241001330002 Bambuseae Species 0.000 description 12
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 12
- 239000011425 bamboo Substances 0.000 description 12
- 230000029087 digestion Effects 0.000 description 9
- 239000000126 substance Substances 0.000 description 6
- 239000003895 organic fertilizer Substances 0.000 description 4
- 238000000053 physical method Methods 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000009941 weaving Methods 0.000 description 2
- 244000302661 Phyllostachys pubescens Species 0.000 description 1
- 235000003570 Phyllostachys pubescens Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01B—MECHANICAL TREATMENT OF NATURAL FIBROUS OR FILAMENTARY MATERIAL TO OBTAIN FIBRES OF FILAMENTS, e.g. FOR SPINNING
- D01B1/00—Mechanical separation of fibres from plant material, e.g. seeds, leaves, stalks
- D01B1/50—Obtaining fibres from other specified vegetable matter, e.g. peat, Spanish moss
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
Landscapes
- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The present invention provides a kind of method for extracting fibrinogen using enzymatic isolation method, this method is specifically used for the extraction of the fibrinogen in oasis No.1 forage, such as lignin etc. is removed by the biological enzyme enzyme digestion reaction of indefinite number, only retain required cellulose, hemicellulose, to reach the extraction effect of fibrinogen, it is specifically exactly the compound action using scouring enzyme and pectase, the enzyme agent of various concentration is used by step, it is reacted under given conditions, it is final to obtain the fibrinogen for retaining various good characteristics, the fibrinogen of extraction can be used for textile preparation, the waste of pollution environment will not be generated simultaneously.
Description
Technical field
The present invention relates to a kind of fibrinogen preparation methods, produce oasis one using enzyme solution in particular to a kind of
The method of number fibrinogen.
Background technique
Bamboo fiber is a kind of natural fiber, it has environmentally friendly, ventilative, nonhazardous specific, is generally acknowledged real meaning
The fiber without chemical component in justice, in recent years, due to preparation method complexity, yield rate is low, either using chemical method,
Or it is physical method, is all difficult to extract the bamboo fiber for meeting weaving requirement, but this does not still prevent people to this
Kind environment-friendly materials are chased.
There are mainly two types of traditional methods for preparing bamboo fibre, and primary provenance and regenerated bamboo fiber, primary provenance is just
It is bamboo fiber, generallys use chemical method, for example Chinese patent CN106995939A discloses a kind of utilization chemical method system
The method for taking Juncao fibrinogen, this method the problem is that the addition of chemical reagent cause it is seriously polluted, in practical operation
Subsequent sewage treatment step expends huge in the process；Another kind is exactly regenerated bamboo fiber, and this method is also using chemistry
Bamboo fibre dissolution is slurried first for method, and the subsequent mode whitewashed is formed, for example Chinese patent CN106958044A is disclosed
A kind of method that producing Juncao pulp fibres using chemical method equally exists offal treatment hardly possible, while this method is produced
Pulp fibres can not show a candle to bamboo fiber environmental protection, it destroys the biological nature of fibrinogen during dissolution, and the slurry produced is fine
Dimension loses many excellent characteristics of fibrinogen.
In view of the above problems, the present invention, in combination with physical method, the main method using biology carries out mentioning for fibrinogen
It takes, for oasis No.1 forage, after analyzing its ingredient, enzyme targetedly is carried out to ingredient unwanted in forage
Solution reaction removal, leaves the bamboo fiber for meeting weaving demand, simultaneously because what is used is biological enzymatic reagent, this method
Residue does not have any toxic action, and can become organic fertilizer well by working process, is real environment-friendly high-efficiency
Fibrinogen preparation method.
In real life, the extraction raw material of bamboo fiber is mainly moso bamboo, and the raw material that the present invention uses is oasis one
Number Juncao, it has a characteristic more excellent than bamboo, for example fibre length is longer, and material is softer, and fibrinogen chromaticness is not adding
It is easier to be accepted in the case where any coloring agent, but is limited by extracting mode, there are no any one to mention for the prior art
The free of contamination mode for taking rate that can reach people's in-mind anticipation emerges, the object of the invention is that providing one kind has high mention
Efficiency is taken, realizes extracting mode relatively satisfactory at this stage in combination with the mode of biology and physical method.
Summary of the invention
In view of this, the present invention provides a kind of method that the method using biological enzymolysis produces oasis No.1 fibrinogen, gram
Clothes are due to extracting fibrinogen bring waste pollution, not tractable defect using chemicals, simultaneously because used
Biological enzyme agent is easily obtained, and so as to which the cost of large-scale production is effectively reduced, and in turn ensures extraction efficiency.In order to realize
Above-mentioned purpose, the invention provides the following technical scheme:
A method of oasis No.1 fibrinogen being produced using enzymatic isolation method, which is characterized in that this method comprises the following steps:
S1: by the interception of oasis No.1 forage at section, clear water is rinsed, and uses cal rolling, at the oasis No.1 forage at section
Manage Cheng Sizhuan；
S2: carrying out clear water boiling processing for the oasis No.1 forage of above-mentioned filiform, filters waste liquid, and clear water cleans filiform and drying
Processing carries out mechanical sub-wire to the filiform using mechanical sub-wire equipment and handles；
S3: using refining enzyme enzyme digestion reaction, according to the desciccate that step S2 is obtained, weighing, according to water-bath ratio 1:(13-17)
Ratio, addition refine enzyme, and first time enzyme digestion reaction 60-90min is carried out at 70-100 DEG C and under concussion, and filtrate is recycled in filtering, clearly
Water cleaning filtering filiform simultaneously press dry sub-wire again；
S4: the drying filiform of sub-wire carries out refining enzyme enzyme digestion reaction, reaction temperature for the second time the process obtained to step S3 again
It is 70-100 DEG C, reaction time 70-90min, filtering, recycling filtrate obtain crude fibre, and dry；
S5: reacted using pectinase enzymatic hydrolysis, according to dry crude fibre: water: pectase=1:(40-55): (0.0024-
0.0032) ratio, controlled at 35-38 degrees Celsius, pH value 5.0-5.3, concussion is enzyme digestion reaction 2.5-3.5 hours lower,
Filtering, recycling filtrate, clear water clean fiber, press dry, and mechanical fine sub-wire dries at 50 DEG C, obtains fibrinogen；
S6: softening weighs softening agents according to the 0.18-0.24% of fibrinogen dry weight, is diluted, is obtained soft with 15-20 times of clear water
Softening agent is uniformly sprayed on fibrinogen by agent, and room temperature seals 8-12 hours, obtains the fibrinogen of softening.
Preferably, in the step S2, clear water boiling is boiling 3-12 hours under conditions of temperature is 95-102 DEG C.
Preferably, the concentration that enzyme is refined in the first time enzyme digestion reaction is 12-15g/L, second of enzyme digestion reaction
The concentration of middle refining enzyme is 6-9g/L.
The softening agents uses carding agent.
Before starting the S1 step, the oasis No.1 of acquisition is carried out to pick leaf processing, only retains bamboo fiber content
High bar.
Compared with prior art, the present invention realizes the degradation to lignin in oasis No.1 using biological enzyme agent, while again
The content of cellulose in forage is not interfered with, high microsteping amount is preferably saved, has not only reduced the generation of pollution waste, but also
Fiber extraction efficiently, economic is reached.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention,
Technical solution in embodiment of the present invention is clearly and completely described, it is clear that described embodiment is the present invention one
Section Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making
Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
The term used in embodiments of the present invention is only to be not intended to be limiting merely for for the purpose of describing particular embodiments
The present invention.In the embodiment of the present invention and the "an" of singular used in the attached claims, " described " and "the"
It is also intended to including most forms, unless the context clearly indicates other meaning, " a variety of " generally comprise at least two, but not
It excludes to include at least one situation.
It will be appreciated that though may be described in embodiments of the present invention using term first, second, third, etc..,
But these ... it should not necessarily be limited by these terms.These terms be only used to by ... distinguish.For example, not departing from implementation of the present invention
In the case where example range, first ... can also be referred to as second ..., and similarly, second ... can also be referred to as the
One ....
It should also be noted that, the terms "include", "comprise" or its any other variant are intended to nonexcludability
Include, so that commodity or system including a series of elements not only include those elements, but also including not clear
The other element listed, or further include for this commodity or the intrinsic element of system.In the feelings not limited more
Under condition, the element that is limited by sentence "including a ...", it is not excluded that in the commodity or system for including the element also
There are other identical elements.
In addition, the step timing in following each method embodiments is only a kind of citing, rather than considered critical.
Embodiment one
Firstly, oasis No.1 forage is carried out to pick leaf processing, section, continuous-flow type clear water are then intercepted by way of machine cuts
Disintegrating slag, the remaining blade etc. of forage are rinsed out, and will be at section by the way of cal rolling by the forage primary stem portion after cleaning
Oasis No.1 forage be processed into filiform；
Secondly, the oasis No.1 forage of above-mentioned filiform is carried out clear water boiling processing, boiling can under high pressure or normal pressure into
Row, digestion time is depending on the growth period of oasis No.1, for example the oasis No.1 of growth three or four months can be small with boiling 3-5
When, and growth period reaches half a year or more, then needs to increase digestion time, boiling temperature is controlled at 98 DEG C, and digestion process terminates
After filter waste liquid, which can not discharge, and can collect into special waste liquid pool, and organic fertilizer, clear water can be made by processing
Cleaning filiform is simultaneously dried, and is then carried out mechanical sub-wire to filiform using mechanical sub-wire equipment and is handled, which can
So that the more clumps of fibers to condense together originally are split off under the intervention of mechanical equipment, thinner fibre bundle is formed；
Again, enzyme digestion reaction is carried out using refining enzyme, the desciccate that above step is obtained is weighed, according to water-bath ratio 1:14's
Ratio, the refining enzyme that addition concentration is 15g/L carry out first time enzyme digestion reaction 65min, filtration residue at 85 DEG C and under concussion
Object, recycling filtrate enter waste liquid pool, and clear water cleaning filtering filiform simultaneously press dry, again using mechanical sub-wire equipment sub-wire；
Then, to obtained process, the drying filiform of sub-wire carries out refining enzyme enzyme digestion reaction for the second time again, this time enzyme digestion reaction
Reaction temperature be 90 DEG C, reaction time 80min, the concentration of scouring enzyme is 8g/L, and filtering, recycling filtrate enter waste liquid pool, passes through
Crude fibre can be obtained by crossing second of enzyme digestion reaction, and obtained crude fibre is dried；
Subsequently, pectinase enzymatic hydrolysis reaction is carried out to the crude fibre after drying, according to dry crude fibre: water: pectase=1:
The ratio of 45:0.0028 shakes enzyme digestion reaction 2.5 hours lower, filtering, recycling controlled at 36 degrees Celsius, pH value 5.2
Filtrate enters waste liquid pool, and clear water cleans fiber, press dry, and carries out the last fine sub-wire of machinery, dries at 50 DEG C, obtains fibril
Dimension；
Finally, carrying out softening processing, carding agent T-8112 is weighed according to the 0.19% of fibrinogen dry weight, it is dilute with 15-20 times of clear water
It releases, obtains softening agent, softening agent is uniformly sprayed on fibrinogen, room temperature seals 8-12 hours, obtains the fibrinogen of softening.
Embodiment two
Firstly, oasis No.1 forage is intercepted into section by way of machine cuts, continuous-flow type clear water rinse out forage disintegrating slag,
Blade etc., and the forage primary stem portion after cleaning will be processed into silk at the oasis No.1 forage of section by the way of cal rolling
Shape；
Secondly, the oasis No.1 forage of above-mentioned filiform is carried out clear water boiling processing, boiling can under high pressure or normal pressure into
Row, digestion time is depending on the growth period of oasis No.1, for example the oasis No.1 of growth three or four months can be small with boiling 3-5
When, and growth period reaches half a year or more, then needs to increase digestion time, boiling temperature is controlled at 100 DEG C, and digestion process terminates
After filter waste liquid, which can not discharge, and can collect into special waste liquid pool, and organic fertilizer, clear water can be made by processing
Cleaning filiform is simultaneously dried, and is then carried out mechanical sub-wire to filiform using mechanical sub-wire equipment and is handled, which can
So that the more clumps of fibers to condense together originally are split off under the intervention of mechanical equipment, thinner fibre bundle is formed；
Again, enzyme digestion reaction is carried out using refining enzyme, the desciccate that above step is obtained is weighed, according to water-bath ratio 1:15's
Ratio, the refining enzyme that addition concentration is 14g/L carry out first time enzyme digestion reaction 75min, filtration residue at 80 DEG C and under concussion
Object, recycling filtrate enter waste liquid pool, and clear water cleaning filtering filiform simultaneously press dry, again using mechanical sub-wire equipment sub-wire；
Then, to obtained process, the drying filiform of sub-wire carries out refining enzyme enzyme digestion reaction for the second time again, this time enzyme digestion reaction
Reaction temperature be 85 DEG C, reaction time 80min, the concentration of scouring enzyme is 8g/L, and filtering, recycling filtrate enter waste liquid pool, passes through
Crude fibre can be obtained by crossing second of enzyme digestion reaction, and obtained crude fibre is dried；
Subsequently, pectinase enzymatic hydrolysis reaction is carried out to the crude fibre after drying, according to dry crude fibre: water: pectase=1:
The ratio of 50:0.0029 shakes enzyme digestion reaction 3 hours lower, filtering, recycling filter controlled at 37 degrees Celsius, pH value 5.1
Liquid enters waste liquid pool, and clear water cleans fiber, press dry, and carries out the last fine sub-wire of machinery, dries at 50 DEG C, obtains fibril
Dimension；
Finally, carrying out softening processing, carding agent T-8112 is weighed according to the 0.20% of fibrinogen dry weight, it is dilute with 15-20 times of clear water
It releases, obtains softening agent, softening agent is uniformly sprayed on fibrinogen, room temperature seals 8-12 hours, obtains the fibrinogen of softening.
Embodiment three
Firstly, oasis No.1 forage is intercepted into section by way of machine cuts, continuous-flow type clear water rinse out forage disintegrating slag,
Blade etc., and the forage primary stem portion after cleaning will be processed into silk at the oasis No.1 forage of section by the way of cal rolling
Shape；
Secondly, the oasis No.1 forage of above-mentioned filiform is carried out clear water boiling processing, boiling can under high pressure or normal pressure into
Row, digestion time is depending on the growth period of oasis No.1, for example the oasis No.1 of growth three or four months can be small with boiling 3-5
When, and growth period reaches half a year or more, then needs to increase digestion time, boiling temperature is controlled at 102 DEG C, and digestion process terminates
After filter waste liquid, which can not discharge, and can collect into special waste liquid pool, and organic fertilizer, clear water can be made by processing
Cleaning filiform is simultaneously dried, and is then carried out mechanical sub-wire to filiform using mechanical sub-wire equipment and is handled, which can
So that the more clumps of fibers to condense together originally are split off under the intervention of mechanical equipment, thinner fibre bundle is formed；
Again, enzyme digestion reaction is carried out using refining enzyme, the desciccate that above step is obtained is weighed, according to water-bath ratio 1:16's
Ratio, the refining enzyme that addition concentration is 16g/L carry out first time enzyme digestion reaction 90min, filtration residue at 95 DEG C and under concussion
Object, recycling filtrate enter waste liquid pool, and clear water cleaning filtering filiform simultaneously press dry, again using mechanical sub-wire equipment sub-wire；
Then, to obtained process, the drying filiform of sub-wire carries out refining enzyme enzyme digestion reaction for the second time again, this time enzyme digestion reaction
Reaction temperature be 80 DEG C, reaction time 70min, the concentration of scouring enzyme is 9g/L, and filtering, recycling filtrate enter waste liquid pool, passes through
Crude fibre can be obtained by crossing second of enzyme digestion reaction, and obtained crude fibre is dried；
Subsequently, pectinase enzymatic hydrolysis reaction is carried out to the crude fibre after drying, according to dry crude fibre: water: pectase=1:
The ratio of 55:0.0031 shakes enzyme digestion reaction 3.5 hours lower, filtering, recycling controlled at 38 degrees Celsius, pH value 5.3
Filtrate enters waste liquid pool, and clear water cleans fiber, press dry, and carries out the last fine sub-wire of machinery, dries at 50 DEG C, obtains fibril
Dimension；
Finally, carrying out softening processing, carding agent T-8112 is weighed according to the 0.23% of fibrinogen dry weight, it is dilute with 15-20 times of clear water
It releases, obtains softening agent, softening agent is uniformly sprayed on fibrinogen, room temperature seals 8-12 hours, obtains the fibrinogen of softening.
Through the above description of the embodiments, those skilled in the art can be understood that each embodiment can
To adjust the range of parameters in suitable range so that extraction process is more efficient, throughout the extraction process, ability
Field technique personnel can also increase or decrease the program of enzyme digestion reaction according to the actual situation, such as can be by first time scouring enzyme enzyme
Solution reaction and second of scouring enzyme enzyme digestion reaction is combined into one, or increase scouring enzyme enzyme digestion reaction number, also can according to
The fibrinogen quality arrived increases pectinase enzymatic hydrolysis number, so that the lignin more effectively adhered to around degradation of fibers is to reach most
Good practical effect.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (6)
1. a kind of method for extracting fibrinogen using enzymatic isolation method, which is characterized in that this method comprises the following steps:
S1: by the interception of oasis No.1 forage at section, clear water is rinsed, and uses cal rolling, at the oasis No.1 forage at section
Manage Cheng Sizhuan；
S2: carrying out clear water boiling processing for the oasis No.1 forage of above-mentioned filiform, filters waste liquid, and clear water cleans filiform and drying
Processing carries out mechanical sub-wire to the filiform using mechanical sub-wire equipment and handles；
S3: using refining enzyme enzyme digestion reaction, according to the desciccate that step S2 is obtained, weighing, according to water-bath ratio 1:(13-17)
Ratio, addition refine enzyme, and first time enzyme digestion reaction 60-90min is carried out at 70-100 DEG C and under concussion, and filtrate is recycled in filtering, clearly
Water cleaning filtering filiform simultaneously press dry sub-wire again；
S4: the drying filiform of sub-wire carries out refining enzyme enzyme digestion reaction, reaction temperature for the second time the process obtained to step S3 again
It is 70-100 DEG C, reaction time 70-90min, filtering, recycling filtrate obtain crude fibre, and dry；
S5: reacted using pectinase enzymatic hydrolysis, according to dry crude fibre: water: pectase=1:(40-55): (0.0024-
0.0032) ratio, controlled at 35-38 degrees Celsius, pH value 5.0-5.3, concussion is enzyme digestion reaction 2.5-3.5 hours lower,
Filtering, recycling filtrate, clear water clean fiber, press dry, and mechanical fine sub-wire dries at 50 DEG C, obtains fibrinogen.
2. the method according to claim 1, wherein this method still further comprises step S6: softening, according to original
The 0.18-0.24% of fiber dry weight weighs softening agents, is diluted with 15-20 times of clear water, obtains softening agent, softening agent is uniformly sprayed
On fibrinogen, room temperature is sealed 8-12 hours, obtains the fibrinogen of softening.
3. the method according to claim 1, wherein clear water boiling is 95-100 in temperature in the step S2
Boiling 3-12 hours under conditions of DEG C.
4. the method according to claim 1, wherein the concentration for refining enzyme in the first time enzyme digestion reaction is
12-15g/L, the concentration that enzyme is refined in second of enzyme digestion reaction is 6-9g/L.
5. according to the method described in claim 2, it is characterized in that, the softening agents uses carding agent.
6. the method according to being strongly required 1, which is characterized in that before step S1, carried out to the oasis No.1 forage of acquisition
Blade cleaning, only retains oasis No.1 bar.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110978171A (en) * | 2019-12-18 | 2020-04-10 | 张明勋 | Preparation method and application of bamboo pulp fiber and bamboo extract from bamboo |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101538744A (en) * | 2009-04-30 | 2009-09-23 | 福建建州竹业科技开发有限公司 | Method for producing bamboo fiber |
| US20110312066A1 (en) * | 2009-01-13 | 2011-12-22 | Sung Wing L | Enzymatic preparation of plant fibers |
| CN106087075A (en) * | 2016-07-12 | 2016-11-09 | 宜宾长顺竹木产业有限公司 | A kind of production method of the bamboo fiber of good spinnability |
| CN106119980A (en) * | 2016-09-09 | 2016-11-16 | 天津工业大学 | A kind of preparation method that becoming more meticulous of meyer sedge fiber |
| CN106995939A (en) * | 2017-03-16 | 2017-08-01 | 王晓翔 | The method that Jujun grasses fibrillation is produced using chemical method |
| US20180105851A1 (en) * | 2016-10-14 | 2018-04-19 | Dalian Sanyuanxin Enzymatic Science & Technology Co., Ltd. | Method and system for preparing pulp for paper with grass straws as raw material |
-
2019
- 2019-09-28 CN CN201910928352.6A patent/CN110453293A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110312066A1 (en) * | 2009-01-13 | 2011-12-22 | Sung Wing L | Enzymatic preparation of plant fibers |
| CN101538744A (en) * | 2009-04-30 | 2009-09-23 | 福建建州竹业科技开发有限公司 | Method for producing bamboo fiber |
| CN106087075A (en) * | 2016-07-12 | 2016-11-09 | 宜宾长顺竹木产业有限公司 | A kind of production method of the bamboo fiber of good spinnability |
| CN106119980A (en) * | 2016-09-09 | 2016-11-16 | 天津工业大学 | A kind of preparation method that becoming more meticulous of meyer sedge fiber |
| US20180105851A1 (en) * | 2016-10-14 | 2018-04-19 | Dalian Sanyuanxin Enzymatic Science & Technology Co., Ltd. | Method and system for preparing pulp for paper with grass straws as raw material |
| CN106995939A (en) * | 2017-03-16 | 2017-08-01 | 王晓翔 | The method that Jujun grasses fibrillation is produced using chemical method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110978171A (en) * | 2019-12-18 | 2020-04-10 | 张明勋 | Preparation method and application of bamboo pulp fiber and bamboo extract from bamboo |
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