CN110447530A - A kind of selection of pale green leaf CMS line, Xian round-grained rice osculant restorer and ideotype breeding - Google Patents
A kind of selection of pale green leaf CMS line, Xian round-grained rice osculant restorer and ideotype breeding Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
This application involves be the selection of ideotype breeding and the selection of sterile line and Xian round-grained rice osculant restorer to breeding of hybridized rice field more particularly to a kind of pale green leaf three.First purpose of the application is to provide the selection that a kind of pale green leaf three is ideotype breeding, this method obtain yield height, leaf color pale green, later period annesl it is good three be ideotype breeding new varieties.Second purpose of the application is to provide a kind of selection of pale green leaf CMS line, and this method selects the pale green leaf CMS line that sterile gene is stable, flowering habit is good, outcrossing seed-setting rate is high, leaf morphology is excellent.The third purpose of the application is to provide a kind of selection of pale green leaf Xian round-grained rice osculant restorer, and this method selects that leaf morphology is good, economical character is excellent, restorative good pale green leaf Xian round-grained rice osculant restorer.
Description
Technical field
This application involves arrive breeding of hybridized rice field more particularly to a kind of pale green leaf CMS line, Xian round-grained rice osculant
The selection of restorer and ideotype breeding.
Background technique
Hybrid rice is extensive in China's plantation in recent years, is widely applied the utilization for mainly having benefited from hybrid vigour, miscellaneous
The yield of rice is handed over to be apparently higher than conventional rice variety.For the producing level of hybrid vigour, due between indica and japonica subspecies heredity away from
From big, the hybrid vigour of ideotype breeding is significantly stronger than that Xian Xian is handed over or round-grained rice round-grained rice is handed over, thus its yield potential is huge.So
And the breeding time of most ideotype breedings is partially long, there is a problem of remaining green when it is due to become yellow and ripe late-maturing, later period annesl difference, is unfavorable for Xian round-grained rice
The popularizing planting of inter-subspecies hybrid rice.In this context, the breeding side that a kind of pale green leaf three is ideotype breeding is invented
Method, the pale green leaf three good for breeding later period annesl is ideotype breeding, improves the remaining green when it is due to become yellow and ripe of ideotype breeding
Late-maturing, later period annesl difference problem, is conducive to the further genralrlization application of ideotype breeding.
This application discloses one kind for Chinese invention patent application (publication number: CN1185898A, publication date: 1998.07.01)
Series of three-series hybrid rice sterile line breeding method with leaf marking trait, it is first with the holding system of ternary hybrid rice and mark
Note seed rice is hybridized, is reestablished diplomatic relations, and fine individual plant is selected with sterile line test cross, to continue to select strain backcrossing respectively in F2, is recycled to the
The sterile line with leaf marking trait can be obtained in nine generations, the seed have unique leaf marking trait, have antiforge function,
Readily identified identification.This method is related to pale green leaf rice and uses as label, is not related to solve the greedy of ideotype breeding
Green late-maturing, later period annesl difference problem.
Summary of the invention
In order to solve the above technical problems, it is indica and japonica subspecies that first purpose of the application, which is to provide a kind of pale green leaf three,
The selection of intermolecular hybrid rice, this method obtain yield height, leaf color pale green, later period annesl it is good three be ideotype breeding
New varieties.
Second purpose of the application is to provide a kind of selection of pale green leaf CMS line, and this method selects not
Educate the pale green leaf CMS line that stability of characteristics, flowering habit are good, outcrossing seed-setting rate is high, leaf morphology is excellent.
The third purpose of the application is to provide a kind of selection of pale green leaf Xian round-grained rice osculant restorer, this method choosing
Bring out that leaf morphology is good, economical character is excellent, restorative good pale green leaf Xian round-grained rice osculant restorer.
In order to realize that first above-mentioned purpose, the application use technical solution below:
A kind of pale green leaf three is the selection of ideotype breeding, which comprises the following steps:
1) pale green leaf genetic resources are imported by non-pale green leaf by breeding technique using pale green leaf rice pest insects material
In sterile line, breeding pale green leaf CMS line；
2) pale green leaf genetic resources are imported by non-pale green leaf by breeding technique using pale green leaf rice pest insects material
In restorer, breeding pale green leaf Xian round-grained rice osculant restorer；
3) it is combined with the sterile line that step 1) obtains with the restorer preparing hybrid that step 2) obtains, obtains yield height, leaf
Color pale green, later period annesl it is good three be ideotype breeding new varieties.
Preferably, breeding technique described in step 1) and step 2) include hybridization, backcrossing or in addition to hybridization, backcrossing its
Its breeding technique.
Preferably, pale green leaf rice pest insects material described in step 1) and step 2) is long-grained nonglutinous rice, japonica rice, the Xian of pale green leaf
Round-grained rice osculant material, wild rice material or the pale green leaf mutant material in other sources.
Preferably, non-pale green leaf CMS line described in step 1) is any kind of sterile line, such as Yunnan I type,
BT type, print water type, Yebai or red lotus type.
Preferably, non-pale green leaf restorer described in step 2) is round-grained rice type, indica type or Xian round-grained rice osculant restorer.
Preferably, the selection of step 1) pale green leaf CMS line the following steps are included:
1.1) select pale green leaf rice pest insects material as receptor or donor parents；
1.2) by with control pale green phyllopodium because parent and non-pale green leaf keep being to hybridize；
1.3) after isolation in generation, the pale green leaf single plant for selecting leaf morphology excellent harvests seed；
1.4) plantation step 1.3) in pale green leaf material, it is continuous to be selfed 3-4 generation, obtain the excellent pale green leaf of leaf morphology
Keep system；
1.5) keep system as recurrent parent using the pale green leaf of breeding, by hybridizing and being returned means, continuous backcross 3-5
Generation, by pale green phyllopodium because importeding into non-pale green leaf CMS line, to obtain pale green leaf CMS line.
Preferably, the selection of step 2) pale green leaf Xian round-grained rice osculant restorer the following steps are included:
2.1) select indica type pale green leaf, round-grained rice type or Xian round-grained rice osculant rice pest insects material as receptor or donor parents；
2.2) by with control pale green phyllopodium because parent and round-grained rice type, indica type or indica-japonica intermediate type variety restorer it is miscellaneous
It hands over；
2.3) in segregating generation, the pale green leaf single plant for selecting leaf morphology excellent harvests seed；
2.4) rice material and different types of sterile line test cross obtained with step 2.3) measures its restorative, selection
The wherein strain that recovery capability is strong, economical character is excellent.
Preferably, the pale green leaf Xian round-grained rice osculant restorer of breeding is the recovery of the inclined Xian of Xian round-grained rice osculant or inclined round-grained rice
System.
In order to realize that second above-mentioned purpose, the application use technical solution below:
A kind of selection of pale green leaf CMS line, method includes the following steps:
1.1) select pale green leaf rice pest insects material as receptor or donor parents；
1.2) by with control pale green phyllopodium because parent and non-pale green leaf keep being to hybridize；
1.3) after isolation in generation, the pale green leaf single plant for selecting leaf morphology excellent harvests seed；
1.4) plantation step 1.3) in pale green leaf material, it is continuous to be selfed 3-4 generation, obtain the excellent pale green leaf of leaf morphology
Keep system；
1.5) keep system as recurrent parent using the pale green leaf of breeding, by hybridizing and being returned means, continuous backcross 3-5
Generation, by pale green phyllopodium because importeding into non-pale green leaf CMS line, to obtain pale green leaf CMS line.
Preferably, the pale green leaf rice pest insects material in the step 1.1) is long-grained nonglutinous rice, japonica rice, the wild rice of pale green leaf
Material or the mutant material in other sources.
Preferably, the pale green leaf rice pest insects material in the step 1.1), the leaf color of vegetative growth phase are in
Light green, naked eyes are distinguishable.
Preferably, non-pale green leaf in the step 1.2) keeps system is Yunnan I type, BT type, print water type, Yebai, red
Lotus type.
Preferably, the non-pale green leaf holding in the step 1.2) is that leaf morphology is good, economical character is excellent.
Preferably, the pale green leaf in the step 1.4) keeps system not separate, inheritance stability.
Preferably, the non-pale green leaf CMS line in the step 1.5) is Yunnan I type, BT type, print water type, open country is lost
Type, red lotus type.
Preferably, the non-pale green leaf CMS line sterile gene in the step 1.5) is stablized, flowering habit is good, different
Hand over setting percentage high, leaf morphology is excellent, leaf color pale green, and naked eyes are distinguishable.
Above-mentioned method by select the rice pest insects material of pale green leaf as parent, by hybridization, continuously be selfed and
The technological means such as continuous backcross, select that sterile gene is stable, flowering habit is good, outcrossing seed-setting rate is high, leaf morphology is excellent
Pale green leaf CMS line.
In order to realize that above-mentioned third purpose, the application use technical solution below:
A kind of selection of pale green leaf Xian round-grained rice osculant restorer, method includes the following steps:
2.1) select indica type pale green leaf, round-grained rice type or Xian round-grained rice osculant rice pest insects material as receptor or donor parents；
2.2) by with control pale green phyllopodium because parent and round-grained rice type, indica type or indica-japonica intermediate type variety restorer it is miscellaneous
It hands over；
2.3) in segregating generation, the pale green leaf single plant for selecting leaf morphology excellent harvests seed；
2.4) rice material and different types of sterile line test cross obtained with step 2.3) measures its restorative, selection
The wherein strain that recovery capability is strong, economical character is excellent.
Preferably, the pale green leaf rice pest insects material in the step 2.1) is in the long-grained nonglutinous rice of pale green leaf, japonica rice, Xian round-grained rice
Between profile material, wild rice material or other sources pale green leaf mutant material.
Preferably, the pale green leaf rice pest insects material in the step 2.1), the leaf color of vegetative growth phase are in
Light green.
Preferably, the non-pale green leaf restorer in the step 2.2) is round-grained rice type, indica type or Xian round-grained rice osculant is restored
System.
Preferably, the non-pale green leaf restorer leaf morphology in the step 2.2) is good, economical character is excellent, restorative
It is good.
Preferably, in the step 2.4) breeding pale green leaf Xian round-grained rice osculant restorer inheritance stability.
Preferably, to can be Xian round-grained rice osculant inclined for the pale green leaf Xian round-grained rice osculant restorer of breeding in the step 2.4)
The restorer of Xian or inclined round-grained rice.
Above-mentioned method by select the rice pest insects material of pale green leaf as parent, by conventional hybridization, continuously oneself
It the technological means such as hands over, selects that leaf morphology is good, economical character is excellent, restorative good pale green leaf Xian round-grained rice osculant restorer.
The selection of the application is suitable for carrying out genetic improvement to existing non-pale green leaf restorer, formulates pale green leaf Xian round-grained rice osculant
Restorer material.
The application passes through conventional hybridization, continuous selfing etc. by selecting the rice pest insects material of pale green leaf as parent
Technological means selects the excellent pale green leaf CMS line of economical character and pale green leaf Xian round-grained rice osculant restorer respectively, and
Hybridization combo is carried out using the sterile line and restorer of institute's breeding, breeding pale green leaf three is ideotype breeding.The application
Selection be suitable for carrying out genetic improvement to existing non-pale green leaf sterile line and restorer, thus breeding pale green leaf Xian round-grained rice
Inter-subspecies hybrid rice new varieties.Using the pale green leaf ideotype breeding new varieties of the application breeding, hence it is evident that improve Xian
Remaining green when it is due to become yellow and ripe late-maturing, later period annesl difference the problem of Via-back puncture intermolecular hybrid rice, is beneficial to the popularization and application of ideotype breeding.
Detailed description of the invention
Fig. 1 is spring river 24A plant.
Fig. 2 is spring river 28A plant.
Specific embodiment
Embodiment 1
Pale green leaf three is the breeding of japonica rice sterile line spring river 24A: the excellent non-pale green leaf japonica rice of selection economical character keeps
It is spring river 26B for female parent, the pale green leaf japonica rice material spring river 19B excellent with comprehensive agronomy character is hybridized, and is selected in F2 generation
The excellent pale green leaf single plant of comprehensive agronomy character was selfed for 4 generations, and heredity is basicly stable, spring river 24B is obtained, with the Yunnan I type sterile line spring
River 16A test cross and 5 generation of continuous backcross, choose that sterile gene is stable, flowering habit is good, outcrossing seed-setting rate is high, leaf morphology is excellent
It is good, the round-grained rice type CMS line spring river 24A of leaf color pale green.
Table 1, spring river 24A are compared with the economical character of spring river 16A
Embodiment 2
Pale green leaf three is the breeding of japonica rice sterile line spring river 28A: the excellent pale green leaf japonica rice material of selection comprehensive agronomy character
Expect that spring river 19B is female parent, keeps being that spring river 028 is hybridized with japonica rice, select comprehensive agronomy character excellent in F2 after hybridization
It is special to choose infertility with Yunnan I type sterile line spring river 16A test cross and 5 generation of continuous backcross after selfing 3 instead of for good pale green leaf single plant
Property is stable, flowering habit is good, outcrossing seed-setting rate is high, leaf morphology is excellent, the round-grained rice type CMS line spring river 28A of leaf color pale green.
Table 2, spring river 28A are compared with the economical character of spring river 16A
Kept being spring river 19B as parent in above-described embodiment 1,2 using pale green leaf japonica rice or as female parent or
As male parent, the round-grained rice type CMS line of leaf color pale green is formulated using the pale green leaf genetic resources that it is carried.In addition to this implementation
Other than pale green leaf rice pest insects spring river 19B used in example, it is also an option that other pale green leaf rice pest insects materials, such as
Long-grained nonglutinous rice, japonica rice, wild rice material or the mutant material in other sources of pale green leaf, use them as parent, can also reach
To same effect.
In addition, all breeding materials involved in the application can be obtained through commercial channels.
Embodiment 3
The breeding of pale green leaf Xian round-grained rice osculant restorer CH101: among the excellent non-pale green leaf Xian round-grained rice of selection economical character
Type restorer CH50 is female parent, and the pale green leaf Xian round-grained rice osculant restorer material C52 excellent with comprehensive agronomy character is hybridized,
The excellent pale green leaf single plant of selection comprehensive agronomy character, continuous to be selfed for 4 generations in F2 generation, inheritance stability strain is obtained, with Yunnan I type
Japonica rice sterile spring river 16A test cross selects restorative strong pale green leaf Xian round-grained rice osculant restorer CH101.
Embodiment 4
The breeding of pale green leaf Xian round-grained rice osculant restorer CH111: the excellent non-pale green leaf long-grained nonglutinous rice of selection economical character restores
It is CH48 for female parent, is hybridized with pale green leaf Xian round-grained rice osculant restorer C52, selection comprehensive agronomy character is excellent in F2 generation
Pale green leaf single plant, it is continuous to be selfed for 5 generations, obtain inheritance stability strain, then with Yunnan I type japonica rice sterile spring river 16A test cross, selection
Restorative strong pale green leaf Xian round-grained rice osculant restorer CH111 out.
Using pale green leaf Xian round-grained rice osculant restorer C52 as parent in above-described embodiment 3,4, the pale green carried using it
Leaf genetic resources formulate the Xian round-grained rice osculant restorer of leaf color pale green.In addition to pale green leaf rice used in the present embodiment provides
Other than the C52 of source, it is also an option that other pale green leaf rice pest insects materials, such as the long-grained nonglutinous rice of pale green leaf, japonica rice, Xian round-grained rice osculant
The pale green leaf mutant material of material, wild rice material or other sources, uses them as parent, also can achieve same
Effect.
Embodiment 5
The breeding in pale green leaf ideotype breeding spring excellent 241: stable with sterile gene, flowering habit is good, outcrossing knot
The pale green leaf round-grained rice type CMS line spring river 24A that real rate is high, leaf morphology is excellent is female parent, with excellent light of comprehensive agronomy character
Greenery Xian round-grained rice osculant restorer material CH101 carries out test cross, and F1 generation nutrient growth is vigorous, leaf color pale green, proper height, hybrid
Advantage is significant, and later period annesl is good.
Embodiment 6
The breeding in pale green leaf ideotype breeding spring excellent 281: stable with sterile gene, flowering habit is good, outcrossing knot
The pale green leaf round-grained rice type CMS line spring river 28A that real rate is high, leaf morphology is excellent is female parent, with excellent light of comprehensive agronomy character
Greenery Xian round-grained rice osculant restorer material CH101 carries out test cross, and F1 generation nutrient growth is vigorous, leaf color pale green, proper height, hybrid
Advantage is significant, and later period annesl is good.
Table 3, pale green leaf ideotype breeding spring be excellent 241, the spring excellent 281 economical character
Pale green leaf sterile line spring river 24A, spring river 28A and Xian round-grained rice osculant restorer CH101 are used in above-described embodiment 5,6
Hybridization, the ideotype breeding new varieties of breeding leaf color pale green.In addition to pale green leaf sterile line used in the present embodiment
Other than restorer, it is also an option that other pale green leaf sterile lines and restorer material, also can achieve same effect.
The above are the descriptions to the embodiment of the present application to keep this field special by the foregoing description of the disclosed embodiments
Industry technical staff can be realized or use the application.Various modifications to these embodiments carry out those skilled in the art
Saying will be apparent.The general principles defined herein can be the case where not departing from spirit herein or range
Under, it realizes in other embodiments.Therefore, the application is not intended to be limited to these implementation columns shown in this article, but to accord with
Close the widest scope consistent with principles disclosed herein and novel point.
Claims (10)
1. the selection that a kind of pale green leaf three is ideotype breeding, which comprises the following steps:
1) pale green leaf rice pest insects material is utilized, by breeding technique, pale green leaf genetic resources are imported into non-pale green leaf infertility
In system, breeding pale green leaf CMS line；
2) pale green leaf rice pest insects material is utilized, by breeding technique, pale green leaf genetic resources is imported into non-pale green leaf and are restored
In system, breeding pale green leaf Xian round-grained rice osculant restorer；
3) it is combined with the sterile line that step 1) obtains with the restorer preparing hybrid that step 2 obtains, it is light to obtain yield height, leaf color
Green, later period annesl it is good three be ideotype breeding new varieties.
2. the selection that a kind of pale green leaf three according to claim 1 is ideotype breeding, which is characterized in that
Breeding technique described in step 1) and step 2 includes hybridization, backcrossing or other breeding techniques in addition to hybridization, backcrossing.
3. the selection that a kind of pale green leaf three according to claim 1 is ideotype breeding, which is characterized in that
Pale green leaf rice pest insects material described in step 1) and step 2 is the long-grained nonglutinous rice of pale green leaf, japonica rice, Xian round-grained rice osculant material, wild
Rice material or the pale green leaf mutant material in other sources.
4. the selection that a kind of pale green leaf three according to claim 1 is ideotype breeding, which is characterized in that
Non- pale green leaf CMS line described in step 1) is any kind of sterile line, such as Yunnan I type, BT type, print water type, Yebai
Or red lotus type.
5. the selection that a kind of pale green leaf three according to claim 1 is ideotype breeding, which is characterized in that
Non- pale green leaf restorer described in step 2 is round-grained rice type, indica type or Xian round-grained rice osculant restorer.
6. a kind of pale green leaf three according to claim 3 or 4 is the selection of ideotype breeding, feature exists
In, step 1) pale green leaf CMS line selection the following steps are included:
1.1) select pale green leaf rice pest insects material as receptor or donor parents；
1.2) by with control pale green phyllopodium because parent and non-pale green leaf keep being to hybridize；
1.3) after isolation in generation, the pale green leaf single plant for selecting leaf morphology excellent harvests seed；
1.4) plantation step 1.3) in pale green leaf material, it is continuous to be selfed 3-4 generation, obtain the excellent pale green leaf of leaf morphology and keep
System；
1.5) keep system continuous backcross 3-5 generation, will by hybridizing and being returned means as recurrent parent using the pale green leaf of breeding
Pale green phyllopodium is because importeding into non-pale green leaf CMS line, to obtain pale green leaf CMS line.
7. a kind of pale green leaf three according to claim 3 or 5 is the selection of ideotype breeding, feature exists
In, step 2) pale green leaf Xian round-grained rice osculant restorer selection the following steps are included:
2.1) select indica type pale green leaf, round-grained rice type or Xian round-grained rice osculant rice pest insects material as receptor or donor parents；
2.2) by with control pale green phyllopodium because parent hybridize with round-grained rice type, indica type or indica-japonica intermediate type variety restorer；
2.3) in segregating generation, the pale green leaf single plant for selecting leaf morphology excellent harvests seed；
2.4) rice material and different types of sterile line test cross obtained with step 2.3), measures that its is restorative, and selection is wherein
The strain that recovery capability is strong, economical character is excellent.
8. a kind of selection of pale green leaf Xian round-grained rice osculant restorer according to claim 7, which is characterized in that breeding
Pale green leaf Xian round-grained rice osculant restorer be the inclined Xian of Xian round-grained rice osculant or inclined round-grained rice restorer.
9. a kind of selection of pale green leaf CMS line, the selection of the pale green leaf CMS line include following step
It is rapid:
1.1) select pale green leaf rice pest insects material as receptor or donor parents；
1.2) by with control pale green phyllopodium because parent and non-pale green leaf keep being to hybridize；
1.3) after isolation in generation, the pale green leaf single plant for selecting leaf morphology excellent harvests seed；
1.4) plantation step 1.3) in pale green leaf material, it is continuous to be selfed 3-4 generation, obtain the excellent pale green leaf of leaf morphology and keep
System；
1.5) keep system continuous backcross 3-5 generation, will by hybridizing and being returned means as recurrent parent using the pale green leaf of breeding
Pale green phyllopodium is because importeding into non-pale green leaf CMS line, to obtain pale green leaf CMS line.
10. a kind of selection of pale green leaf Xian round-grained rice osculant restorer, the breeding side of the pale green leaf Xian round-grained rice osculant restorer
Method the following steps are included:
2.1) select indica type pale green leaf, round-grained rice type or Xian round-grained rice osculant rice pest insects material as receptor or donor parents；
2.2) by with control pale green phyllopodium because parent hybridize with round-grained rice type, indica type or indica-japonica intermediate type variety restorer；
2.3) in segregating generation, the pale green leaf single plant for selecting leaf morphology excellent harvests seed；
2.4) rice material and different types of sterile line test cross obtained with step 2.3), measures that its is restorative, and selection is wherein
The strain that recovery capability is strong, economical character is excellent.
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| 贾良等: "带叶色标记晚粳稻的农艺性状及其在育种中的应用评价 ", 《安徽农业科学》 * |
| 高志宏等: "籼粳杂交水稻新品种春优984的选育 ", 《种子》 * |
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