CN110438006B - Wall-attached single-cell double-layer culture device - Google Patents
Wall-attached single-cell double-layer culture device Download PDFInfo
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- CN110438006B CN110438006B CN201910895033.XA CN201910895033A CN110438006B CN 110438006 B CN110438006 B CN 110438006B CN 201910895033 A CN201910895033 A CN 201910895033A CN 110438006 B CN110438006 B CN 110438006B
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- 230000001464 adherent effect Effects 0.000 claims abstract description 55
- 239000011521 glass Substances 0.000 claims abstract description 31
- 230000021164 cell adhesion Effects 0.000 claims description 4
- 239000007787 solid Substances 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 5
- 238000013461 design Methods 0.000 abstract description 4
- 230000005484 gravity Effects 0.000 abstract description 4
- 238000011534 incubation Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 58
- 230000011278 mitosis Effects 0.000 description 7
- 238000012258 culturing Methods 0.000 description 6
- 108010019160 Pancreatin Proteins 0.000 description 5
- 229940055695 pancreatin Drugs 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002547 new drug Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 238000004115 adherent culture Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
Abstract
The invention discloses an adherent single-cell double-layer culture device which comprises a slide glass, an inserting sheet, a pulling sheet, a driving strip, a carrier and a solid column, wherein the driving strip is positioned at the uppermost part of the device, the pulling sheet is arranged below the driving strip, the inserting sheet comprises an inserting sheet side wing, a top cover and a hinge area, the bottom of the inserting sheet is provided with a supporting column for preventing the adhesion of the two wings, the inserting sheet side wing is connected with the top cover through the hinge area, the slide glass is a hollow slide glass and comprises a carrier groove, an edge hole, a carrier groove bracket, an inner slide glass outer frame, an upper support rib and a lower support rib, the solid column is a rectangular column for fixing the slide glass, and the carrier is a three-dimensional frame comprising an edge column and a solid column clamping groove. According to the complex device for the double-layer culture of the adherent single cells, the earth gravity and the adherent growth characteristics of the adherent single cells are utilized, and double cells obtained by the first division of the adherent single cells are adhered to the mirror-image inserting piece flanks through vertical incubation and inverted culture. The device has the advantages of fine structure, ingenious design, simple and convenient operation, high double-layer culture efficiency, and convenience for laboratory research and aseptic operation.
Description
Technical Field
The invention belongs to the technical field of general chemistry or physical laboratory equipment, and particularly relates to an adherent single-cell double-layer culture device.
Background
Adherent cells (adherends) are a class of eukaryotic cell lines that rely on self-secretion, culture fluid supply, or adhesion factors coated on the surface of a support to grow and proliferate on the surface of the support. The growth process of adherent cells is mainly divided into a free phase, an adherent phase, a latent phase, a logarithmic phase, a plateau phase and a decay phase, and an epithelial cell-like or fibroblast-like growth morphology is generally formed after adherence. The adherent cells become important laboratory materials for researching vaccines, developing new drugs, preparing monoclonal antibodies, analyzing gene functions and drug action mechanisms, culturing pathogenic microorganisms in vitro, and ascertaining the occurrence and development mechanisms of diseases and the self-activity rules of the adherent cells, and the scientific research value of the adherent cells is not light.
At present, the common adherent cell culture method comprises the following steps: the cells to be passaged are blown into single cell suspension after being digested by pancreatin, then are paved into a cell bottle or a culture plate according to a specified inoculation proportion, and are placed into a cell incubator to continue the nutrition adherence growth until the experimental requirement is met or the bottom surface of the support is fully distributed. It is not difficult to find that the adherent cells obtained by the passage method are cell groups obtained by dividing the adherent single cells with unknown numbers through multiple times of mitosis, and the cell spacing between the adherent single cells in each cell bottle or culture plate is not equal except that the initial cell laying number and the mitosis times are different. The obtained adherent cells are also a cell population which has uniform morphology, stable growth and clear biological properties, and the reproductive activity and the metabolic capability of the adherent cells are obviously different.
Disclosure of Invention
In order to solve the technical problems, the invention provides an adherent single-cell double-layer culture device which comprises a slide glass, an inserting piece, a poking piece, a driving strip, a carrier and a fixed column, wherein the driving strip is positioned above the device and comprises a strip head and a strip body; the plectrum is positioned below the driving strip and comprises a sheet body, a clamping part, a plectrum ring, a rotary column and a rotary column base, wherein the plectrum ring is a buckle for connecting the inserting sheet, the plectrum can rotate relative to the inserting sheet, and the sheet body is a slide for poking the side wings of the inserting sheet; the inserting piece comprises an inserting piece side wing, a top cover and a hinge area, a supporting column for preventing the double wings from adhering is arranged at the bottom of the side wing, the pressure edge is positioned on the inner side above the side wing, the height of the pressure edge is the same as that of the supporting column, and the inserting piece side wing is connected with the top cover through the hinge area; the slide glass is a hollow slide glass and comprises a carrier groove, an edge hole, a carrier groove bracket, an inner slide glass outer frame, an upper supporting edge and a lower supporting edge, wherein the edge hole is used for inserting an edge column, and the supporting edge is a square column for supporting the slide glass, assisting in fixing an inserting piece and keeping the distance between adjacent slide glass; the fixed column is a rectangular column for fixing a slide glass; the carrier is a three-dimensional frame comprising edge columns and fixing column clamping grooves.
Preferably, the driving strips are double strips with opposite movement directions, the strip heads are pinching parts, the surfaces of the strip heads are provided with anti-skidding patterns, the strip bodies are provided with round strip holes, and the number of the strip bodies is the same as that of the driven poking strips.
Preferably, the rotating column of the poking piece is a round column inserted into the bar hole, the height is the same as the thickness of the driving bar, and the diameter is smaller than that of the rotating base; the outer diameter of the rotary column is slightly smaller than the inner diameter of Yu Tiaokong, and the driving bar and the poking plate can rotate around the rotary column.
Preferably, the clamping part of the plectrum is positioned in the middle of the upper part of the plectrum, the height of the clamping part is the same as that of the rotating base, and the clamping part is used for clamping and lifting by hands.
Preferably, the middle part of the top cover is provided with two poking plate jacks, and four corners are respectively provided with a round jacking column.
Preferably, the side wings of the inserting sheet are left and right double wings which are mirror images, and the inner side surfaces of the double wings are provided with adherent cell adhesion areas.
Preferably, the carrier groove is a U-shaped channel groove capable of carrying only a single adherent cell.
The invention provides a novel device for culturing adherent single cells in a double-layer mode and a novel feasibility scheme for culturing the adherent single cells in the double-layer mode.
According to the design of the earth gravity and the adherent cell adherent growth characteristics, firstly, a carrier with a slide is immersed into an adherent cell suspension, and is taken out after 3-5s of action; inserting an inserting sheet and a poking sheet, installing a driving bar and a fixing column, standing upside down the culture device until the first mitosis is completed after the cells are completely adhered; and (3) taking out the solid column after connection between the pancreatin digested double cells, pulling out the inserting sheet forward, and unfolding and culturing. The invention has fine structure, ingenious design and high wall-attached single-cell double-layer separation efficiency, and is convenient for laboratory research and aseptic operation.
Drawings
The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.
FIG. 1 is a perspective view of an adherent single-cell double-layered culture apparatus according to an embodiment of the invention
FIG. 2 is a top view of an adherent single-cell double-layered culture apparatus according to an embodiment of the invention.
FIG. 3 is a perspective view of an adherent single-cell double-layered culture apparatus according to an embodiment of the invention.
FIG. 4 is a schematic cross-sectional view of an adherent single-cell double-layered culture apparatus according to an embodiment of the invention.
Fig. 5 is a top view of a slide.
FIG. 6 is a schematic view of a longitudinal section and a cross section of a slide.
Fig. 7 is an erect, abducted, and abducted top schematic view of the tab.
Fig. 8 is a perspective view of the dial.
Fig. 9 is a schematic longitudinal section of the pulling piece.
Fig. 10 is a schematic structural view of the driver blade.
Fig. 11 is a perspective view of the fixing post.
Fig. 12 is a perspective view of the carrier.
FIG. 13 is a schematic diagram showing an operation flow of an adherent single-cell double-layered culture apparatus according to an embodiment of the invention.
In the figure, 1: a slide; 2: inserting sheets; 3: a pulling piece; 4: driving the strip; 5: a carrier; 6: fixing the column; 11: a carrying groove; 12: a carrier groove bracket; 13: slide inner frame; 14: an upper supporting edge; 15: a lower supporting edge; 16: slide glass outer frame; 17: an edge hole; 18: a cell bearing region; 21: a side wing of the insert; 22: abutting the column; 23: pressing edges; 24: a top cover; 25: a top column; 26: a plectrum jack; 27: an adhesion zone; 28: a hinge region; 31: a sheet body; 32: a clamping part; 33: a paddle ring; 34: a spin column; 35: a swivel post base; 41: a strip head; 42: a strip body; 43: a strip hole; 44: anti-skid lines; 51: edge posts; 52: a fixing column clamping groove.
Detailed Description
As shown in figures 1-13, the invention discloses an adherent single-cell double-layer culture device, which is a slide combined container for double-layer culture of adherent single cells.
The adherence is the characteristic that the adherence factors secreted by cells, provided by culture solution or coated on the surface of a cell support can grow and reproduce on the surface of the support.
The single cell is a single cell group obtained by digestion of adherent cells to be passaged with pancreatin.
The double-layer culture is a culture mode of mirror image separation of double cells obtained by first mitosis of single cells by utilizing the earth gravity and the adherent growth characteristics of adherent cells.
The container is a vitreous container.
An adherent single-cell double-layer culture device comprises a slide glass 1, an inserting sheet 2, a poking sheet 3, a driving bar 4, a carrier 5 and a fixed column 6, wherein the driving bar 4 is positioned above the device and comprises a bar head 41 and a bar body 42; the plectrum 3 is located below the driving bar 4, and comprises a sheet body 31, a clamping part 32, a plectrum ring 33, a rotating column 34 and a rotating column base 35, wherein the plectrum ring 33 is a buckle for connecting the inserting sheet 2, the plectrum can rotate relative to the inserting sheet, and the sheet body 31 is a slide for poking the inserting sheet flank 21; the inserting sheet 2 comprises an inserting sheet side wing 21, a top cover 24 and a hinge area 28, a supporting column 22 for preventing the double wings from adhering is arranged at the bottom of the side wing, a pressing edge 23 is positioned on the upper inner side of the side wing, the height of the pressing edge is the same as that of the supporting column 25, the inserting sheet side wing 21 is connected with the top cover 24 through the hinge area 28, and the hinge can be made of a soft material capable of being folded back; the lower part of the sheet body is positioned below the top cover 24 and is used for poking the inserting sheet side wings 21; the slide glass 1 is a hollow slide glass and comprises a slide groove 11, an edge hole 17, a slide groove bracket 12, slide glass inner frames 13 and 16, upper and lower supporting ribs 14 and 15, wherein the edge hole 17 is used for inserting an edge column 51, and the supporting ribs are square columns for supporting the slide glass 1, assisting in fixing the inserting sheet 2 and keeping the distance between the adjacent slide glass 1; the fixed column 6 is a rectangular column for fixing the slide glass 1; the carrier 5 is a three-dimensional frame comprising an edge post 51 and a post fixing clamping groove 52.
The driving strips 4 are double strips with opposite movement directions, the strip heads 41 are pinching parts, the surfaces of the strip heads are provided with anti-slip patterns 44, the strip bodies 42 are provided with round strip holes 43, and the number of the strip holes is the same as that of the driven poking sheets 3.
The rotary column 34 of the plectrum 3 is a round column inserted into the bar hole 43, the height is the same as the thickness of the driving bar 4, and the diameter is smaller than the rotary base; the outer diameter of the rotary column 34 is slightly smaller than the inner diameter of the strip hole 43, and the drive strip and the poking piece can rotate around the rotary column.
The clamping part 32 of the plectrum 3 is positioned in the middle of the upper part of the plectrum 3, the height is the same as that of the rotating base, and the clamping part 32 is used for clamping and lifting by hands.
The middle part of the top cover 24 is provided with two poking plate jacks 26, and four corners are respectively provided with a round top column 25.
The inserting piece side wings 21 are left and right double wings which are mirror images, and the inner sides of the double wings are provided with adherent cell adhesion areas 27.
The carrying groove 11 is a U-shaped channel groove which can only carry single adherent cells.
The operation flow is as follows:
1. preparing an adherent single-cell suspension;
1) Removing upper culture solution when the adherent cells are fully distributed on the bottom surface of the support, adding appropriate amount of 0.25% pancreatin solution, and standing for CO 2 In the incubator, the cells were digested until they completely shed.
2) Adding appropriate amount of cell culture solution (containing 10% fetal bovine serum and 1 Xdouble antibody), gently mixing, blowing to single cell state, and transferring into sterile 50mL centrifuge tube, 6-hole or 12-hole culture plate for use.
2. Assembling and disassembling the device and culturing cells;
1) Slide 1 is sequentially loaded into carriers 5 along edge posts 51, and the carriers loaded with slides are vertically immersed in the single cell suspension for 3-5s and then taken out.
2) The two wings of the inserting piece are carefully inserted into the side grooves of the lower supporting edges (the area between the lower supporting edges and the inner frame 13 of the slide glass), and the pulling piece 3 is arranged (the direction of the sheet body 31 is parallel to the side wings of the inserting piece 2) and then pushed downwards to the bottom of the carrier 5.
3) The tab 2 is then pushed forward as indicated in fig. 13 to the upper support rib 14, and the wings of the tab 2 are held against the intermediate carrier groove 11 with sterile forceps.
4) The insert 2 was pushed forward into the side groove of the upper support rib 14 (the region between the upper support rib 14 and the carrier groove bracket 12), and after the solid column 6 was inserted into the solid column clamping groove 52, incubation and culture were continued in a proper amount of cell culture solution (containing 10% fetal bovine serum and 1×diab).
5) After the cells are fully adherent, the device is inverted and cultured until the first mitosis of the cells is completed.
6) After the double cells are connected by digestion with a proper amount of pancreatin, the solid column 6 is taken out, the inserting sheet 2 is pulled back to the lower supporting edge, and the driving bar 4 is assembled and drawn to the two sides.
7) When the side wings of the inserting sheet 2 are abutted against the slide inner frame 13, the driving strip 4 is detached, and the inserting sheet 2 is pulled back into the lower supporting edge side groove.
8) The pulling piece 3 is taken out, the inserting piece 2 is pulled out upwards, the inserting piece 2 is placed in a cell bottle or a culture plate, and the cell adhesion area 27 is unfolded and cultured upwards.
9) According to the research requirements, if a large number of adherent cells are needed, the side wing size of the inserting sheet can be increased and/or the number of the carrying sheets 1 and 2 can be increased.
The adherent cells are eukaryotic cell lines which can grow and propagate on the surface of the support by means of self secretion, culture solution supply or adhesion factors coated on the surface of the support, and become important laboratory materials for researching vaccines, developing new drugs, preparing monoclonal antibodies, analyzing gene functions and drug action mechanisms, culturing pathogenic microorganisms in vitro and ascertaining the occurrence and development mechanisms of diseases and the self-activity rule of the adherent cells. By using the traditional passage way, the obtained adherent cells are cell groups obtained by carrying out multiple mitosis on unknown adherent single cells, and besides the initial cell laying number and the mitosis frequency are different, the cell spacing between the adherent single cells in each cell bottle or culture plate is also different, so that the reproductive activity and the metabolism capability of the obtained adherent cells are obviously different. The invention provides a complex device for double-layer culture of adherent single cells, which utilizes the earth gravity and the adherent growth characteristics of the adherent single cells to adhere the double cells obtained by first division of the adherent single cells to mirror image inserting sheet flanks through vertical incubation and inverted culture. The device has the advantages of fine structure, ingenious design, simple and convenient operation, high double-layer separation efficiency, and convenience for laboratory research and aseptic operation.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (1)
1. An adherent single-cell divides double-deck culture apparatus, its characterized in that: the device comprises a slide glass (1), an inserting piece (2), a poking piece (3), a driving bar (4), a carrier (5) and a fixed column (6), wherein the driving bar (4) is positioned above the device and comprises a bar head (41) and a bar body (42); the plectrum (3) is positioned below the driving strip (4) and comprises a sheet body (31), a clamping part (32), a plectrum ring (33), a rotating column (34) and a rotating column base (35), wherein the plectrum ring (33) is a buckle for connecting the inserting sheet (2), the plectrum can rotate relative to the inserting sheet, and the sheet body (31) is a glass slide for poking a side wing (21) of the inserting sheet; the inserting piece (2) comprises an inserting piece side wing (21), a top cover (24) and a hinge area (28), a supporting column (22) for preventing the double wings from adhering is arranged at the bottom of the side wing, a pressing edge (23) is positioned on the inner side above the side wing, and the inserting piece side wing (21) is connected with the top cover (24) through the hinge area (28); the slide glass (1) is a hollow slide glass and comprises a slide glass groove (11), edge holes (17), a slide glass bracket (12), slide glass inner frames (13, 16) and upper and lower supporting ribs (14, 15), wherein the edge holes (17) are used for inserting edge columns (51), and the supporting ribs are square columns for supporting the slide glass (1), assisting in fixing inserting sheets (2) and keeping the distance between adjacent slide glass (1); the fixed column (6) is a rectangular column for fixing the slide glass (1); the carrier (5) is a three-dimensional frame comprising edge columns (51) and a fixed column clamping groove (52);
the driving strips (4) are double strips with opposite movement directions, the strip heads (41) are pinching parts, anti-slip patterns (44) are formed on the surfaces of the strip heads, the strip bodies (42) are provided with round strip holes (43), and the number of the strip bodies is the same as that of the driven poking sheets (3);
the rotary column (34) of the poking piece (3) is a round column inserted into the bar hole (43), the height is the same as the thickness of the driving bar (4), and the diameter is smaller than the rotary base; the outer diameter of the rotary column (34) is slightly smaller than the inner diameter of the strip hole (43), and the driving strip (4) and the poking piece (3) can rotate around the rotary column;
the clamping part (32) of the plectrum (3) is positioned in the middle of the upper part of the plectrum (3), the height of the clamping part is the same as that of the rotating base, and the clamping part (32) is used for clamping and lifting by hands;
two poking plate jacks (26) are arranged in the middle of the top cover (24), and round jacking columns (25) are respectively arranged at four corners of the top cover;
the inserting piece side wings (21) are left and right double wings which are mirror images, and the inner side surfaces of the double wings are provided with adherent cell adhesion areas (27);
the carrying groove (11) is a U-shaped channel groove which can only carry single adherent cells.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910895033.XA CN110438006B (en) | 2019-09-20 | 2019-09-20 | Wall-attached single-cell double-layer culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910895033.XA CN110438006B (en) | 2019-09-20 | 2019-09-20 | Wall-attached single-cell double-layer culture device |
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| CN110438006A CN110438006A (en) | 2019-11-12 |
| CN110438006B true CN110438006B (en) | 2024-03-01 |
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| CN201910895033.XA Active CN110438006B (en) | 2019-09-20 | 2019-09-20 | Wall-attached single-cell double-layer culture device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112980689A (en) * | 2021-02-08 | 2021-06-18 | 湖南美柏生物医药有限公司 | Adherent cell culture device, 2.5D beehive type culture system and method |
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| RU2668157C1 (en) * | 2017-11-23 | 2018-09-26 | Общество с ограниченной ответственностью научно-технический центр "БиоКлиникум" (ООО НТЦ "БиоКлиникум") | Device for formation of a two-layer cellular model |
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| US4939151A (en) * | 1988-10-31 | 1990-07-03 | Baxter International Inc. | Adherent cell culture flask |
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| CN110438006A (en) | 2019-11-12 |
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