CN110426262A - A kind of amino acid sample-processing equipment and detection method - Google Patents
A kind of amino acid sample-processing equipment and detection method Download PDFInfo
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- CN110426262A CN110426262A CN201910830012.XA CN201910830012A CN110426262A CN 110426262 A CN110426262 A CN 110426262A CN 201910830012 A CN201910830012 A CN 201910830012A CN 110426262 A CN110426262 A CN 110426262A
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- component
- hydrolysis
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- amino acid
- pipe
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
Abstract
The invention discloses a kind of amino acid sample-processing equipment and detection methods, belong to amino acid detection device technical field, it is successively set on pulverizing-grinding unit on support bearing component, hydrolysis processing component including support bearing component, along detection ordering, sufficiently washes component, positioning Knock-Down Component and carrying components of assays, connecting pipe is equipped between the hydrolysis processing component and sufficiently washing component, the bottom of the connecting pipe is equipped with locating rack, and the bottom of the locating rack is connect with support bearing component.The invention also discloses a kind of amino acid sample detection methods.The present invention is reduced in the detection process by the preparation step of a series of automation, and artificial output improves detection efficiency, avoids the generation of risk during the experiment.
Description
Technical field
The present invention relates to amino acid detection device technical fields, more particularly, to a kind of amino acid sample-processing equipment and inspection
Survey method.
Background technique
Amino acid is the compound after hydrogen atom on carboxylic acid carbon atom is replaced by amino, contains ammonia in amino acid molecular
Base and carboxyl Liang Zhong functional group.It is similar with carboxylic acid, different location that amino acid can be connected in carbochain according to amino and be divided into α-,
β-, γ-... w- amino acid, but the amino acid obtained after protein hydrolyzes is all a-amino acid, and only twenties kinds,
They are the basic units for constituting protein.Amino acid is the base substance of protein needed for constituting Animal nutrition.It is containing alkali
The organic compound of property amino and acidic carboxypolymer.It is a-amino acid that amino, which is connected on α-carbon,.The amino acid of constitutive protein matter is big
Part is a-amino acid.Amino acid can play following some effects: 1. synthetic tissue protein by metabolism in human body；
2. becoming acid, hormone, antibody, creatine etc. containing ammoniacal substance；3. being changed into carbohydrate and fat；4. be oxidized to carbon dioxide and
Water and urea generate energy.
In the prior art, to amino acid carry out detection process in, be all according to operator according to detecting step successively
Test tube, bearing material bottle and hydrolysis pipe etc. are moved, different conditions and the reactant of differential responses are moved back and forth, influence to detect
Efficiency also increases the risk of experiment, influences final detection effect.
Summary of the invention
The purpose of the present invention is to provide a kind of amino acid sample-processing equipment and detection methods, to solve in the prior art
The technical issues of.
The present invention provides a kind of amino acid sample-processing equipment, including support bearing component, successively sets along detection ordering
It sets the pulverizing-grinding unit on support bearing component, hydrolysis processing component, sufficiently wash component, positioning Knock-Down Component and carrying
Components of assays is equipped with connecting pipe between the hydrolysis processing component and sufficiently washing component, and the bottom of the connecting pipe is set
There is locating rack, the bottom of the locating rack is connect with support bearing component.
Further, the support bearing component includes grinding supporting table and is transversely mounted platform, described to be transversely mounted platform and grind
It grinds supporting table to be arranged on same level straight line, the side for being transversely mounted platform and grinding supporting table being arranged in, the crushing is ground
Mill component is mounted on the top of grinding supporting table, and the hydrolysis processing component sufficiently washes component, positioning Knock-Down Component and carrying
Components of assays is installed in the top for being transversely mounted platform.
Further, the pulverizing-grinding unit includes for carrying out crushing and the fixation of grinding operation to food solid sample
The top of grinding supporting table, the High Purity Nitrogen gas steel is arranged in grinding barrel and High Purity Nitrogen gas bomb, the fixed grinding barrel
Bottle is mounted on the side of grinding supporting table.
Further, the hydrolysis processing component includes positioning supporting frame, process support frame, carrying receiving bottle, sample reagent
Component and hydrolysis processing assembly is added, the carrying accommodates on the table top of bottle insertion process support frame, and the sample reagent is added
Component is arranged on the table top of process support frame, and the discharge end of component and the feed end pair of carrying receiving bottle is added in sample reagent
It connects, the hydrolysis processing assembly is erected on positioning supporting frame, and the hydrolysis processing assembly accommodates bottle by pipeline and carrying and connects
It is logical.
Further, it includes L shape positioning plate, driving cylinder, location and installation frame, hydrochloric acid input that component, which is added, in the sample reagent
Pipe, phenol input pipe, sample input pipe and two extending columns, the driving cylinder are mounted on the top of L shape positioning plate, two institutes
The two sides that driving cylinder is arranged in extending column are stated, it is described to drive the end of cylinder and two extending columns through L shape positioning plate and determine
Position installing frame is fixedly connected, and the hydrochloric acid input pipe, phenol input pipe and sample input pipe are arranged in location and installation frame
Between position.
Further, the hydrolysis processing component further includes hydrolysis pipe, nitrogen access tube, high temperature heater (HTH) and two aqueous electricity
The underface of hydrolysis pipe is arranged in magnet valve, the high temperature heater (HTH), and the hydrolysis pipe is connected to the bottom that carrying accommodates bottle, and two
The both ends of hydrolysis pipe are arranged in the aqueous solenoid valve, and the nitrogen access tube is plugged on hydrolysis pipe above and communicates therewith, described
Sufficiently washing component includes water inlet line, the first control valve, the second control valve, outlet conduit, volumetric flask and flask support frame, institute
State water inlet line be plugged on hydrolysis pipe top and communicate therewith, first control valve is mounted on water inlet line, it is described go out
One end of waterpipe is connected to one end that the separate carrying of hydrolysis pipe accommodates bottle, and second control valve is set in outlet conduit
On, the other end of the outlet conduit is connected to volumetric flask, and the volumetric flask is fixedly mounted on the top of flask support frame, described
Nitrogen access tube is connected to High Purity Nitrogen gas bomb.
Further, the positioning Knock-Down Component includes locating support table, is dried under reduced pressure case and accommodates test tube, and the decompression is dry
Dry case is arranged on locating support table, and the receiving test tube setting is being dried under reduced pressure in case, the side chamber door for being dried under reduced pressure case
It can open.
Further, the carrying components of assays includes measuring positioning plate, measurement cylinder, measurement posting, sodium citrate to delay
Fliud flushing enters pipeline and two measurement fixed columns, and the measurement cylinder is mounted on the top of measurement positioning plate, two measurements
Fixed column is separately positioned on the two sides of measurement cylinder, the output end of the measurement cylinder and the equal edge in end of two measurement fixed columns
Vertical direction through measurement positioning plate and with measurement posting be fixedly connected, the sodium citrate buffer solution enters Pipe installing
At the intermediate position of measurement posting.
The invention also discloses a kind of amino acid sample detection methods,
S1. it takes food solid sample to crush or grind, if it is fluid sample, then beats sealing freezen protective with refiner;
S2. sample is added in carrying receiving bottle and adds hydrochloric acid hydrolysis.
Hydrolysing step specifically: add 10 milliliters of 6mol/L hydrochloric acid by hydrochloric acid input pipe；If sample is liquid, be added etc.
The concentrated hydrochloric acid of volume then instills 3-4 drop phenol by hydrochloric acid input pipe；After hydrolyzing pipe freezing, vacuumize, inflated with nitrogen is taken out very again
Sky 3 times repeatedly, is then sealed, is hydrolyzed 22 hours under 110 degrees Celsius, cooling；
S3. water lotion is filtered, the repeated multiple times flushing hydrolysis pipe of a small amount of water is added by water inlet line, water lotion accumulation is all put into
In 50 milliliters of volumetric flasks, it is settled to 50 milliliters；
S4. it takes in 1 milliliters of liquid to 10 milliliters of receiving test tubes, entire receiving test tube is put into and is dried under reduced pressure in case, is taken the photograph in 40-50
It is dried under reduced pressure under family name's degree, residue is dissolved with 1-2 milliliters of ultrapure waters, then is dried under reduced pressure, and is finally evaporated.
S5. the interior dissolution of test tube mixes after drying is added in sodium citrate buffer solution, draws 1 milliliter of solution and is filtered by 0.22 micron
After film, sample measurement liquid is made.
Compared with prior art, the beneficial effects of the present invention are: first according to the state of sample to be tested, if it is
Solid just passes through fixed grinding barrel and carries out comminution process to it, just passes through refiner if it is liquid and beats liquid, sample
After the completion of preparatory processing, carrying is added in sample and is accommodated in bottle, passes through 10 milliliters of hydrochloric acid of hydrochloric acid input pipe 6mol/L；If sample is
Isometric concentrated hydrochloric acid is then added in liquid, then instills 3-4 drop phenol by hydrochloric acid input pipe；After hydrolyzing pipe freezing, take out true
Sky, inflated with nitrogen vacuumize again, 3 times repeatedly, then seal, are heated to hydrolyzing under 110 degrees Celsius by temperature by high temperature heater (HTH)
It 22 hours, then cools down, the repeated multiple times flushing hydrolysis pipe of a small amount of water is added by water inlet line, water lotion accumulation is all put into 50
In milliliter volumetric flask, 50 milliliters are settled to, is taken in 1 milliliters of liquid to 10 milliliters of receiving test tubes, entire receiving test tube is put into and is subtracted
It presses in drying box, is dried under reduced pressure under 40-50 degrees Celsius, residue is dissolved with 1-2 milliliters of ultrapure waters, then is dried under reduced pressure, finally
It is evaporated, is then mixed, drawn by being dissolved in test tube after sodium citrate buffer solution pipeline addition sodium citrate buffer solution to drying
After solution passes through 0.22 micron membrane filter, sample measurement liquid is made, the preparation of measurement liquid is completed, passes through the system of a series of automation
Standby step, reduces in the detection process, artificial output improves detection efficiency, avoids the production of risk during the experiment
It is raw.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is schematic perspective view of the invention；
Fig. 2 is top view of the invention；
Fig. 3 is the cross-sectional view in Fig. 2 along line A-A；
Fig. 4 is sectional perspective structural schematic diagram of the invention；
Fig. 5 is the partial enlargement diagram that component is added in sample reagent in Fig. 4；
Fig. 6 is the partial enlargement diagram that components of assays is carried in Fig. 4.
Appended drawing reference:
Support bearing component 1 grinds supporting table 1a, is transversely mounted platform 1b, pulverizing-grinding unit 2, fixed grinding barrel 2a, High Purity Nitrogen
Gas bomb 2b hydrolyzes processing component 3, positioning supporting frame 3a, process support frame 3b, and carrying accommodates bottle 3c, and sample reagent is added
Component 3d, L shape positioning plate 3d1, drives cylinder 3d2, location and installation frame 3d3, hydrochloric acid input pipe 3d4, extending column 3d5, and phenol is defeated
Enter pipe 3d6, sample input pipe 3d7, hydrolyze processing assembly 3f, hydrolyzes pipe 3g, nitrogen access tube 3h, high temperature heater (HTH) 3i, aqueous
Solenoid valve 3j sufficiently washes component 4, water inlet line 4a, the first control valve 4b, the second control valve 4c, outlet conduit 4d, volumetric flask
4e, flask support frame 4f position Knock-Down Component 5, and locating support table 5a is dried under reduced pressure case 5b, accommodates test tube 5c, carry determination part
Part 6 measures positioning plate 6a, measures cylinder 6b, measures posting 6c, and sodium citrate buffer solution enters pipeline 6d, measures fixed column
6e, connecting pipe 7, locating rack 8.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is a part of the embodiment of the present invention, instead of all the embodiments.
The component for the embodiment of the present invention for usually describing and showing in attached drawing here can be with a variety of different configurations
To arrange and design.Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below and is wanted
The scope of the present invention of protection is sought, but is merely representative of selected embodiment of the invention.
Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" be based on the orientation or positional relationship shown in the drawings, merely to
Convenient for description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation,
It is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.In addition, term " first ", " second ",
" third " is used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood at this with concrete condition
Concrete meaning in invention.
Below with reference to shown in Fig. 1 to Fig. 4, the embodiment of the invention provides a kind of amino acid sample-processing equipments, including branch
Support load bearing component 1, the pulverizing-grinding unit 2 being successively set on along detection ordering on support bearing component 1, hydrolysis processing component
3, abundant washing component 4, positioning Knock-Down Component 5 and carrying components of assays 6, the hydrolysis processing component 3 and abundant washing component 4
Between be equipped with connecting pipe 7, the bottom of the connecting pipe 7 is equipped with locating rack 8, the bottom of the locating rack 8 and support bearing
Component 1 connects, and working principle: first according to the state of sample to be tested, just passes through fixed grinding barrel 2a if it is solid to it
Carry out comminution process, just pass through refiner 2b if it is liquid and beat liquid, after the completion of the preparatory processing of sample, by sample plus
Enter carrying to accommodate in bottle 3c, adds 10 milliliters of 6mol/L hydrochloric acid by hydrochloric acid input pipe 3d4；If sample is liquid, the bodies such as addition
Long-pending concentrated hydrochloric acid then instills 3-4 drop phenol by hydrochloric acid input pipe 3d4；After hydrolyzing pipe 3g freezing, vacuumize, inflated with nitrogen is again
It vacuumizes, 3 times repeatedly, then seals, temperature is heated to hydrolyzing 22 hours under 110 degrees Celsius by high temperature heater (HTH) 3i, so
After cool down, repeated multiple times flushings of a small amount of water is added by water inlet line 4a and hydrolyzes pipe 3g, water lotion accumulation whole is put into 50 milliliters
In volumetric flask 4e, 50 milliliters are settled to, is taken in 1 milliliters of liquid to 10 milliliters of receiving test tube 5c, the entire test tube 5c that accommodates is put into
It being dried under reduced pressure in case 5b, is dried under reduced pressure under 40-50 degrees Celsius, residue is dissolved with 1-2 milliliters of ultrapure waters, then is dried under reduced pressure,
It is finally evaporated, is then mixed by being dissolved in test tube after sodium citrate buffer solution pipeline addition sodium citrate buffer solution to drying,
1 milliliter of solution is drawn by after 0.22 micron membrane filter, making sample measurement liquid, the preparation of measurement liquid is completed, by a series of
The preparation step of automation reduces in the detection process, and artificial output improves detection efficiency, avoids in experimentation
The generation of risk.
The support bearing component 1 includes grinding supporting table 1a and is transversely mounted platform 1b, described to be transversely mounted platform 1b and grind
It grinds supporting table 1a to be arranged on same level straight line, the side for being transversely mounted platform 1b and grinding supporting table 1a being arranged in is described
Pulverizing-grinding unit 2 is mounted on the top of grinding supporting table 1a, the hydrolysis processing component 3, sufficiently washing component 4, positioning point
Solution component 5 and carrying components of assays 6 are installed in the top for being transversely mounted platform 1b, to realize that the carrying to main process apparatus is made
Industry.
The pulverizing-grinding unit 2 includes the fixation grinding barrel for carrying out crushing and grinding operation to food solid sample
The top of grinding supporting table 1a, the High Purity Nitrogen gas is arranged in 2a and High Purity Nitrogen gas bomb 2b, the fixed grinding barrel 2a
Steel cylinder 2b is mounted on the side of grinding supporting table, the preparation operation of complete paired samples.
The hydrolysis processing component 3 includes positioning supporting frame 3a, process support frame 3b, carrying receiving bottle 3c, sample reagent
Component 3d and hydrolysis processing assembly 3f is added, the carrying accommodates on the table top of bottle 3c insertion process support frame 3b, the sample
Reagent is added component 3d and is arranged on the table top of process support frame 3b, and the discharge end of component 3d is added in sample reagent and carrying accommodates
The feed end docking of bottle 3c, the hydrolysis processing assembly 3f are erected on positioning supporting frame 3a, and the hydrolysis processing assembly 3f is logical
Piping accommodates bottle 3c with carrying and is connected to.
It includes L shape positioning plate 3d1, driving cylinder 3d2, location and installation frame 3d3, hydrochloric acid that component 3d, which is added, in the sample reagent
Input pipe 3d4, phenol input pipe 3d6, sample input pipe 3d7 and two extending columns 3d5, the driving cylinder 3d2 are mounted on L shape
The two sides of driving cylinder 3d2, the driving cylinder 3d2 and two is arranged in the top of positioning plate 3d1, two extending column 3d5
The end of a extending column 3d5 is fixedly connected through L shape positioning plate 3d1 with location and installation frame 3d3, the hydrochloric acid input pipe 3d4, benzene
Phenol input pipe 3d6 and sample input pipe 3d7 is arranged at the intermediate position of location and installation frame 3d3, then is able to achieve to sample reagent
Component 3d is added and component 3e is added for phenol and the one-to-one correspondence of sample is added, completes being continuously added for detection reagent.
The hydrolysis processing component 3 further includes hydrolysis pipe 3g, nitrogen access tube 3h, high temperature heater (HTH) 3i and two aqueous electricity
The underface of hydrolysis pipe 3g is arranged in magnet valve 3j, the high temperature heater (HTH) 3i, and the hydrolysis pipe 3g and carrying accommodate the bottom of bottle 3c
The both ends of hydrolysis pipe 3g are arranged in portion's connection, two aqueous solenoid valve 3j, and the nitrogen access tube 3h is plugged on hydrolysis pipe
3g is upper and communicates therewith, and the abundant washing component 4 includes water inlet line 4a, the first control valve 4b, the second control valve 4c, water outlet
Pipeline 4d, volumetric flask 4e and flask support frame 4f, the water inlet line 4a are plugged on the top of hydrolysis pipe 3g and communicate therewith, institute
It states the first control valve 4b to be mounted on water inlet line 4a, one end of the outlet conduit 4d and the separate carrying of hydrolysis pipe 3g accommodate
One end connection of bottle 3c, the second control valve 4c are set on outlet conduit 4d, the other end and appearance of the outlet conduit 4d
Measuring bottle 4e connection, the volumetric flask 4e are fixedly mounted on the top of flask support frame 4f, the nitrogen access tube 3h and High Purity Nitrogen
Gas bomb 2b connection then needs two aqueous electricity because hydrolysis pipe 3g needs several sections during the work time as sealing state
Magnet valve 3j is sealed operation to entire hydrolysis pipe 3g, improves the efficiency of hydrolysis operation.
The positioning Knock-Down Component 5 includes locating support table 5a, is dried under reduced pressure case 5b and accommodates test tube 5c, and the decompression is dry
Dry case 5b is arranged on locating support table 5a, and the receiving test tube 5c setting is being dried under reduced pressure in case 5b, described to be dried under reduced pressure case
The side chamber door of 5b can be opened, and be dried under reduced pressure case 5b and be able to achieve carrying operation to test tube 5c is accommodated, the decompression of complete paired samples is dry
Dry operation.
The carrying components of assays 6 includes measuring positioning plate 6a, measurement cylinder 6b, measurement posting 6c, sodium citrate to delay
Fliud flushing enters pipeline 6d and two measurement fixed column 6e, and the measurement cylinder 6b is mounted on the top of measurement positioning plate 6a, and two
The measurement fixed column 6e is separately positioned on the two sides of measurement cylinder 6b, and the output end of the measurement cylinder 6b and two measurements are solid
The end of fixed column 6e is fixedly connected each along vertical direction through measurement positioning plate 6a and with measurement posting 6c, the citric acid
Sodium buffer enters the intermediate position that pipeline 6d is mounted on measurement posting 6c.
Detection method,
S1. it takes food solid sample to crush or grind, if it is fluid sample, then beats sealing freezen protective with refiner 2b;
S2. sample is added in carrying receiving bottle 3c and adds hydrochloric acid hydrolysis.
Hydrolysing step specifically: add 10 milliliters of 6mol/L hydrochloric acid by hydrochloric acid input pipe 3d4；If sample is liquid, plus
Enter isometric concentrated hydrochloric acid, 3-4 drop phenol is then instilled by hydrochloric acid input pipe 3d4；After hydrolyzing pipe 3g freezing, vacuumizes, fill
Nitrogen vacuumizes again, 3 times repeatedly, then seals, hydrolyzes 22 hours under 110 degrees Celsius, cooling；
S3. water lotion is filtered, the repeated multiple times flushing of a small amount of water is added by water inlet line 4a and hydrolyzes pipe 3g, water lotion accumulation is all
It is put into 50 milliliters of volumetric flask 4e, is settled to 50 milliliters；
S4. it takes in 1 milliliters of liquid to 10 milliliters of receiving test tube 5c, entire receiving test tube 5c is put into and is dried under reduced pressure in case 5b, In
It is dried under reduced pressure under 40-50 degrees Celsius, residue is dissolved with 1-2 milliliters of ultrapure waters, then is dried under reduced pressure, and is finally evaporated.
S5. the interior dissolution of test tube mixes after drying is added in sodium citrate buffer solution, draws 1 milliliter of solution and is filtered by 0.22 micron
After film, sample measurement liquid is made.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (9)
1. a kind of amino acid sample-processing equipment, which is characterized in that successively including support bearing component (1), along detection ordering
The pulverizing-grinding unit (2) that is arranged on support bearing component (1), sufficiently washes component (4), positioning at hydrolysis processing component (3)
Knock-Down Component (5) and carrying components of assays (6) are equipped with connection between the hydrolysis processing component (3) and sufficiently washing component (4)
The bottom of pipeline (7), the connecting pipe (7) is equipped with locating rack (8), the bottom of the locating rack (8) and support bearing component
(1) it connects.
2. a kind of amino acid sample-processing equipment according to claim 1, which is characterized in that the support bearing component
It (1) include grinding supporting table (1a) and being transversely mounted platform (1b), it is described to be transversely mounted platform (1b) and grinding supporting table (1a) setting
On same level straight line, the side for being transversely mounted platform (1b) setting in grinding supporting table (1a), the smashing and grinding portion
Part (2) is mounted on the top of grinding supporting table (1a), and the hydrolysis processing component (3) sufficiently washes component (4), positioning decomposition
Component (5) and carrying components of assays (6) are installed in the top for being transversely mounted platform (1b).
3. a kind of amino acid sample-processing equipment according to claim 2, which is characterized in that the pulverizing-grinding unit
(2) include for food solid sample carry out crush and grinding operation fixation grinding barrel (2a) and High Purity Nitrogen gas bomb
(2b), fixed grinding barrel (2a) setting is in the top of grinding supporting table (1a), High Purity Nitrogen gas bomb (2b) installation
In the side of grinding supporting table.
4. a kind of amino acid sample-processing equipment according to claim 3, which is characterized in that the hydrolysis processing component
It (3) include positioning supporting frame (3a), process support frame (3b), carrying accommodates bottle (3c), component (3d) and water is added in sample reagent
It solves processing assembly (3f), the carrying accommodates on the table top of bottle (3c) insertion process support frame (3b), and the sample reagent is added
Component (3d) is arranged on the table top of process support frame (3b), and the discharge end of component (3d) is added in sample reagent and carrying accommodates bottle
The feed end of (3c) docks, and the hydrolysis processing assembly (3f) is erected on positioning supporting frame (3a), the hydrolysis processing assembly
(3f) accommodates bottle (3c) with carrying by pipeline and is connected to.
5. a kind of amino acid sample-processing equipment according to claim 4, which is characterized in that the sample reagent addition group
Part (3d) includes L shape positioning plate (3d1), drives cylinder (3d2), location and installation frame (3d3), hydrochloric acid input pipe (3d4), phenol defeated
Enter pipe (3d6), sample input pipe (3d7) and two extending columns (3d5), the driving cylinder (3d2) is mounted on L shape positioning plate
The top of (3d1), two extending columns (3d5) settings in the two sides of driving cylinder (3d2), the driving cylinder (3d2) and
The end of two extending columns (3d5) is fixedly connected through L shape positioning plate (3d1) with location and installation frame (3d3), the hydrochloric acid input
Pipe (3d4), phenol input pipe (3d6) and sample input pipe (3d7) are arranged at the intermediate position of location and installation frame (3d3).
6. a kind of amino acid sample-processing equipment according to claim 5, which is characterized in that the hydrolysis processing component
It (3) further include hydrolysis pipe (3g), nitrogen access tube (3h), high temperature heater (HTH) (3i) and two aqueous solenoid valves (3j), the height
The underface of (3g) is managed in warm heater (3i) setting in hydrolysis, and (3g) is managed in the hydrolysis and the bottom of carrying receiving bottle (3c) connects
Logical, at the both ends of hydrolysis pipe (3g), the nitrogen access tube (3h) is plugged on hydrolysis for two aqueous solenoid valve (3j) settings
It on pipe (3g) and communicates therewith, the abundant washing component (4) includes water inlet line (4a), the first control valve (4b), the second control
Valve (4c), outlet conduit (4d), volumetric flask (4e) and flask support frame (4f) processed, the water inlet line (4a) are plugged on hydrolysis pipe
It the top of (3g) and communicates therewith, first control valve (4b) is mounted on water inlet line (4a), the outlet conduit (4d)
One end with hydrolysis pipe (3g) separate carrying receiving bottle (3c) one end be connected to, second control valve (4c) is set in water outlet
On pipeline (4d), the other end of the outlet conduit (4d) is connected to volumetric flask (4e), and the volumetric flask (4e) is fixedly mounted on
The top of flask support frame (4f), the nitrogen access tube (3h) are connected to High Purity Nitrogen gas bomb (2b).
7. a kind of amino acid sample-processing equipment according to claim 6, which is characterized in that the positioning Knock-Down Component
(5) include locating support table (5a), be dried under reduced pressure case (5b) and accommodate test tube (5c), it is described to be dried under reduced pressure case (5b) setting fixed
In position supporting table (5a), receiving test tube (5c) setting is being dried under reduced pressure in case (5b), the side for being dried under reduced pressure case (5b)
Chamber door can be opened.
8. a kind of amino acid sample-processing equipment according to claim 7, which is characterized in that the carrying components of assays
(6) enter pipeline (6d) including measurement positioning plate (6a), measurement cylinder (6b), measurement posting (6c), sodium citrate buffer solution
With two measurement fixed columns (6e), measurement cylinder (6b) is mounted on the top of measurement positioning plate (6a), two measurements
Fixed column (6e) is separately positioned on the two sides of measurement cylinder (6b), and the output end of measurement cylinder (6b) and two measurements are fixed
The end of column (6e) is fixedly connected each along vertical direction through measurement positioning plate (6a) and with measurement posting (6c), the lemon
Lemon acid sodium buffer enters the intermediate position that pipeline (6d) is mounted on measurement posting (6c).
9. a kind of amino acid sample detection methods, which is characterized in that
S1. it takes food solid sample to crush or grind, if it is fluid sample, then beats sealing freezing with refiner (2b) and protect
It deposits;
S2. sample is added in carrying receiving bottle (3c) and adds hydrochloric acid hydrolysis；
Hydrolysing step specifically: pass through 10 milliliters of hydrochloric acid of hydrochloric acid input pipe (3d4) 6mol/L；If sample is liquid, be added etc.
The concentrated hydrochloric acid of volume then instills 3-4 drop phenol by hydrochloric acid input pipe (3d4)；After hydrolysis pipe (3g) freezing, vacuumizes, fill
Nitrogen vacuumizes again, 3 times repeatedly, then seals, hydrolyzes 22 hours under 110 degrees Celsius, cooling；
S3. water lotion is filtered, the repeated multiple times flushing hydrolysis pipe (3g) of a small amount of water, water lotion accumulation are added by water inlet line (4a)
It is all put into 50 milliliters of volumetric flasks (4e), is settled to 50 milliliters；
S4. it takes in 1 milliliters of liquid to 10 milliliters of receiving test tubes (5c), will entirely accommodate test tube (5c) and put into and be dried under reduced pressure case (5b)
It is interior, it is dried under reduced pressure under 40-50 degrees Celsius, residue is dissolved with 1-2 milliliters of ultrapure waters, then is dried under reduced pressure, and is finally evaporated；
S5. the interior dissolution of test tube mixes after drying is added in sodium citrate buffer solution, draws 1 milliliter of solution and passes through 0.22 micron membrane filter
Afterwards, sample measurement liquid is made.
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