CN110423678B - Soil extracellular enzyme detection device - Google Patents
Soil extracellular enzyme detection device Download PDFInfo
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- CN110423678B CN110423678B CN201811309768.1A CN201811309768A CN110423678B CN 110423678 B CN110423678 B CN 110423678B CN 201811309768 A CN201811309768 A CN 201811309768A CN 110423678 B CN110423678 B CN 110423678B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/38—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a soil extracellular enzyme detection device, and relates to the technical field of soil detection. This extracellular enzyme detection device of soil, including main part mechanism and oscillation mechanism, main part mechanism is including bearing box, fixed slot, fixed cover, cushion washer, separating tube, heater, gluey ring, and the fixed slot is seted up in the centre of bearing box top, and the bottom fixed connection of fixed cover is in the centre of bearing box top, and cushion washer sets up at the middle part of fixed cover inner chamber, and the separating tube setting is in the centre of fixed cover top, and the heater setting is in the centre of bearing box bottom, oscillation mechanism includes motor, mount, axis of rotation, loading board, eccentric rotor, first bearing, annular rotation groove, second bearing, rotation groove. According to the soil extracellular enzyme detection device, soil and extracellular enzyme are completely separated through vibration, the activity of the enzyme is maintained through a heat preservation method, a solid foundation is provided for further determination, and the determination accuracy is improved.
Description
Technical Field
The invention relates to the technical field of soil detection, in particular to a soil extracellular enzyme detection device.
Background
The biochemical technology can be used for rapidly detecting the activity of the soil extracellular enzyme, but the soil enzyme measuring method does not have a standard parameter and a measuring standard so far, and cannot provide an accurate answer to the ecological function of soil microorganisms, soil scholars develop a series of soil microorganism biomass measuring methods such as a direct microscopic examination method, an ATP analysis method, a fumigation culture method, a fumigation extraction method, a substrate induced respiration method and the like, the measuring methods mostly need to use soil educt to carry out measurement, and the common liquid taking method cannot well separate out the enzyme in the soil completely, so that the inconvenience is brought to the measurement.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the soil extracellular enzyme detection device, after the detection device is used, soil and extracellular enzyme are completely separated through oscillation, the activity of the enzyme is kept through a heat preservation method, a solid foundation is provided for further determination, and the determination accuracy is improved.
In order to realize the purpose, the invention is realized by the following technical scheme: the utility model provides a soil ectoenzyme detection device, includes main part mechanism and vibrates the mechanism, main part mechanism is including bearing box, fixed slot, fixed cover, cushion washer, separator tube, heater, gluey ring, the centre at bearing box top is seted up to the fixed slot, the centre at bearing box top is connected to the bottom fixed connection of fixed cover, cushion washer sets up the middle part at fixed cover inner chamber, the separator tube sets up the centre at fixed cover top, the heater sets up the centre at the bearing box bottom, gluey ring sets up the bottom at fixed slot inner chamber.
Vibrate mechanism includes motor, mount, axis of rotation, loading board, eccentric rotor, first bearing, annular rotation groove, second bearing, rotation groove, the mount cup joints the outside at the motor, the centre of the terminal fixed connection in the axis of rotation bottom of motor top output shaft, the quantity of loading board is two, the quantity of first bearing is two, two first bearing sets up the middle part at two loading board tops respectively, eccentric rotor cup joints at the middle part of axis of rotation, the quantity that the annular rotated the groove is two, two the annular rotates the groove and sets up respectively at the both ends of axis of rotation, the second bearing cup joints at the top of axis of rotation.
Preferably, the inner chamber of fixed cover is linked together with the external world by the centre of its top and bottom, the front, the back and the left and right sides of the fixed cover inner wall of the front, the back and the left and right sides laminating of cushion washer and with the inner wall fixed connection of fixed cover, the inside that cushion washer and extension to the fixed slot is run through to the bottom of separator tube, the separator tube laminates with the wall body of cushion washer mutually.
Preferably, the top of heater runs through the centre of bearing box bottom and extends to the inside of fixed slot, the heater is connected with the wall body fixed connection of bearing box, the front of gluing the ring, the back and the left and right sides run through the inner wall of fixed slot and extend to the inside of bearing box inner chamber, glue the ring and be connected with the wall body fixed connection of bearing box, the bottom of separator tube runs through the bottom of gluing the ring and extending to the fixed slot inner chamber and laminates mutually with the top of heater, the separator tube laminates mutually with the wall body of gluing the ring.
Preferably, the number of the oscillation mechanisms is two, two the motors are respectively arranged at the left side and the right side of the inner cavity of the bearing box, the bottom of the motor is fixedly connected with the bottom of the inner wall of the bearing box, the motor is attached to the wall body of the fixing frame, the bottom of the inner wall of the bearing box is fixedly connected with the bottom of the fixing frame, the eccentric rotor is fixedly connected with the rotating shaft, and one end, away from the axis, of the eccentric rotor is attached to the rubber ring.
Preferably, the bottom of first bearing runs through the top of loading board and extends to the outside of loading board bottom, the wall body fixed connection of first bearing and loading board, the top of axis of rotation runs through in proper order runs through two first bearings and extends to the outside of top loading board, the wall body of first bearing inner circle extends to the inside in annular rotating groove, the wall body of first bearing inner circle and the front, the back and the left and right sides of annular rotating inslot wall are laminated and fixed connection mutually, eccentric rotor sets up between two loading boards.
Preferably, two the left and right sides at bearing box inner wall top is seted up to the rotation groove symmetry, two the top of axis of rotation extends to the inside in two rotation grooves respectively, the axis of rotation passes through the key-type connection with the second bearing, the front, the back and the left and right sides laminating rotation inslot wall of second bearing outer wall front, back and left and right sides and with the inner wall fixed connection who rotates the groove.
The invention provides a soil extracellular enzyme detection device. The method has the following beneficial effects:
(1) This soil ectoenzyme detection device through the cooperation work of the carrier case, fixed slot, separator tube, heater, rubber ring, motor, axis of rotation, the eccentric rotor that set up, makes soil and ectoenzyme separate completely through shaking to keep the activity of enzyme through the heat preservation gimmick, for further survey provides solid basis, improved the degree of accuracy of survey.
(2) This soil ectoenzyme detection device, through the cooperation work that bears case, fixed cover, cushion washer, mount, loading board, first bearing, annular rotation groove, second bearing, rotation groove that set up, guarantee that eccentric rotor can be steady produce vibrations to the rubber ring, provide strong support and solid basis for eccentric rotor's rotation, improved this soil ectoenzyme detection device's practicality.
Drawings
FIG. 1 is a schematic structural view in elevation of the present invention;
FIG. 2 is an enlarged view of the invention at A in FIG. 1.
In the figure: the device comprises a main body mechanism 1, a bearing box 11, a fixing groove 12, a fixing sleeve 13, a buffer gasket 14, a separation pipe 15, a heater 16, a rubber ring 17, a vibration mechanism 2, a motor 21, a fixing frame 22, a rotating shaft 23, a bearing plate 24, an eccentric rotor 25, a first bearing 26, an annular rotating groove 27, a second bearing 28 and a rotating groove 29.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative and intended to explain the present invention and should not be construed as limiting the present invention.
Referring to fig. 1-2, the present invention provides a technical solution: a soil extracellular enzyme detection device comprises a main mechanism 1 and a vibration mechanism 2, wherein the main mechanism 1 comprises a bearing box 11, a fixing groove 12, a fixing sleeve 13, a buffer gasket 14, a separation pipe 15, a heater 16 and a rubber ring 17, the fixing groove 12 is arranged in the center of the top of the bearing box 11, the bottom of the fixing sleeve 13 is fixedly connected in the center of the top of the bearing box 11, the buffer gasket 14 is arranged in the middle of the inner cavity of the fixing sleeve 13, the separation pipe 15 is arranged in the center of the top of the fixing sleeve 13, the heater 16 is arranged in the center of the bottom of the bearing box 11, the rubber ring 17 is arranged at the bottom of the inner cavity of the fixing groove 12, the inner cavity of the fixing sleeve 13 is communicated with the outside through the centers of the top and the bottom of the fixing sleeve, the front, the back and the left and right sides of the front, back of the buffer gasket 14 are fixedly connected with the inner wall of the fixing sleeve 13, the separation pipe 15 penetrates through the buffer gasket 14 and extends into the inner wall of the fixing groove 12, the separation pipe 15 is attached to the top of the buffer gasket 14, the top of the bottom of the bearing box 11 and the inner wall of the heating box 11, the heater 16 is connected with the fixing groove 12, the heater 17, the inner wall of the heater 17, the heater 11 and the inner cavity of the support ring 17, the support ring 17 extends into the bearing box 11, the middle of the bearing box 11, and the inner cavity of the support ring 17.
The oscillating mechanism 2 comprises a motor 21, a fixed frame 22, a rotating shaft 23, bearing plates 24, eccentric rotors 25, first bearings 26, annular rotating grooves 27, second bearings 28 and rotating grooves 29, wherein the fixed frame 22 is sleeved outside the motor 21, the tail end of an output shaft at the top of the motor 21 is fixedly connected to the center of the bottom of the rotating shaft 23, the number of the bearing plates 24 is two, the number of the first bearings 26 is two, the two first bearings 26 are respectively arranged in the middle of the tops of the two bearing plates 24, the eccentric rotors 25 are sleeved in the middle of the rotating shaft 23, the number of the annular rotating grooves 27 is two, the two annular rotating grooves 27 are respectively arranged at two ends of the rotating shaft 23, the second bearings 28 are sleeved on the top of the rotating shaft 23, the number of the oscillating mechanism 2 is two, the two motors 21 are respectively arranged at the left side and the right side of an inner cavity of the bearing box 11, the bottom of the motor 21 is fixedly connected to the bottom of the inner wall of the bearing box 11, the motor 21 is attached to the wall body of the fixed frame 22, the bottom of the fixed frame 22 is fixedly connected to the bottom of the inner wall of the bearing box 11, the eccentric rotor 25 is fixedly connected to the rotating shaft 23, one end of the eccentric rotor 25, which is far away from the axis, is attached to the rubber ring 17, through the matched work of the bearing box 11, the fixed groove 12, the separating tube 15, the heater 16, the rubber ring 17, the motor 21, the rotating shaft 23 and the eccentric rotor 25, the soil is completely separated from the extracellular enzyme through oscillation, the activity of the enzyme is maintained through a heat preservation method, a solid foundation is provided for further determination, the determination accuracy is improved, the bottom of the first bearing 26 penetrates through the top of the bearing plate 24 and extends to the outside of the bottom of the bearing plate 24, the first bearing 26 is fixedly connected to the wall body of the bearing plate 24, the top of the rotating shaft 23 penetrates through the two first bearings 26 in sequence and extends to the outside of the bearing plate 24, the wall body of the inner ring of the first bearing 26 extends to the inside of the annular rotating groove 27, the wall body of the inner ring of the first bearing 26 is attached to and fixedly connected with the front, the back and the left and right sides of the inner wall of the annular rotating groove 27, the eccentric rotor 25 is arranged between the two bearing plates 24, the two rotating grooves 29 are symmetrically arranged on the left and right sides of the top of the inner wall of the bearing box 11, the tops of the two rotating shafts 23 respectively extend to the inside of the two rotating grooves 29, the rotating shafts 23 are connected with the second bearing 28 through keys, the front, the back and the left and right sides of the outer wall of the second bearing 28 are attached to the front, the back and the left and right sides of the inner wall of the rotating groove 29, and the eccentric rotor 25 can stably generate vibration on the rubber ring 17 through the matched work of the bearing box 11, the fixed sleeve 13, the buffer washer 14, the fixed frame 22, the bearing plates 24, the first bearing 26, the annular rotating groove 27, the second bearing 28 and the rotating groove 29, so that a powerful support and a firm foundation are provided for the rotation of the eccentric rotor 25, and the practicability of the soil ectoenzyme detection device is improved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. The utility model provides a soil ectoenzyme detection device, includes main part mechanism (1) and vibrates mechanism (2), its characterized in that: the main body mechanism (1) comprises a bearing box (11), a fixing groove (12), a fixing sleeve (13), a buffer gasket (14), a separating pipe (15), a heater (16) and a rubber ring (17), wherein the fixing groove (12) is formed in the center of the top of the bearing box (11), the bottom of the fixing sleeve (13) is fixedly connected to the center of the top of the bearing box (11), the buffer gasket (14) is arranged in the middle of the inner cavity of the fixing sleeve (13), the separating pipe (15) is arranged in the center of the top of the fixing sleeve (13), the heater (16) is arranged in the center of the bottom of the bearing box (11), and the rubber ring (17) is arranged at the bottom of the inner cavity of the fixing groove (12);
the oscillating mechanism (2) comprises a motor (21), a fixed frame (22), a rotating shaft (23), bearing plates (24), an eccentric rotor (25), first bearings (26), annular rotating grooves (27), second bearings (28) and rotating grooves (29), wherein the fixed frame (22) is sleeved outside the motor (21), the tail end of an output shaft at the top of the motor (21) is fixedly connected to the center of the bottom of the rotating shaft (23), the number of the bearing plates (24) is two, the number of the first bearings (26) is two, the two first bearings (26) are respectively arranged in the middle of the tops of the two bearing plates (24), the eccentric rotor (25) is sleeved in the middle of the rotating shaft (23), the number of the annular rotating grooves (27) is two, the two annular rotating grooves (27) are respectively arranged at two ends of the rotating shaft (23), and the second bearings (28) are sleeved at the top of the rotating shaft (23);
the inner chamber of fixed cover (13) is linked together with the external world by the centre of its top and bottom, the front, the back and the left and right sides of fixed cover (13) inner wall of the laminating of the front, the back and the left and right sides of cushion washer (14) and with the inner wall fixed connection of fixed cover (13), the inside of cushion washer (14) and extension fixed slot (12) is run through and the bottom of separator tube (15), separator tube (15) laminate with the wall body of cushion washer (14) mutually.
2. The soil extracellular enzyme detection device according to claim 1, wherein: the centre of bearing box (11) bottom is run through and the inside of extending to fixed slot (12) in the top of heater (16), heater (16) and the wall body fixed connection of bearing box (11), the front, the back and the left and right sides of gluing ring (17) run through the inner wall of fixed slot (12) and extend to the inside of bearing box (11) inner chamber, glue ring (17) and the wall body fixed connection of bearing box (11), the bottom of separator tube (15) is run through and is glued ring (17) and extend to the bottom of fixed slot (12) inner chamber and laminate mutually with the top of heater (16), separator tube (15) laminate mutually with the wall body of gluing ring (17).
3. The soil extracellular enzyme detection device according to claim 2, wherein: the quantity of oscillating mechanism (2) is two, two motor (21) set up respectively in the left and right sides of bearing box (11) inner chamber, on the bottom of the bottom fixed connection bearing box (11) inner wall of motor (21), the wall body of motor (21) and mount (22) is laminated mutually, on the bottom of the bottom fixed connection bearing box (11) inner wall of mount (22), eccentric rotor (25) and axis of rotation (23) fixed connection, the one end that the axle center was kept away from in eccentric rotor (25) is laminated mutually with gluey ring (17).
4. The soil extracellular enzyme detection device according to claim 3, wherein: the bottom of first bearing (26) runs through the top of loading board (24) and extends to the outside of loading board (24) bottom, the wall body fixed connection of first bearing (26) and loading board (24), the top of axis of rotation (23) is run through two first bearings (26) in proper order and is extended to the outside of top loading board (24), the wall body of first bearing (26) inner circle extends to the inside of annular rotation groove (27), the wall body of first bearing (26) inner circle and the front of annular rotation groove (27) inner wall, the back and the left and right sides paste mutually and fixed connection, eccentric rotor (25) set up between two loading boards (24).
5. The soil extracellular enzyme detection device according to claim 4, wherein: two the left and right sides at bearing box (11) inner wall top is seted up to rotation groove (29) symmetry, two the top of axis of rotation (23) extends to the inside of two rotation grooves (29) respectively, axis of rotation (23) passes through the key-type connection with second bearing (28), the front, the back and the left and right sides laminating of second bearing (28) outer wall rotate front, the back and the left and right sides of groove (29) inner wall and with the inner wall fixed connection who rotates groove (29).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811309768.1A CN110423678B (en) | 2018-11-05 | 2018-11-05 | Soil extracellular enzyme detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811309768.1A CN110423678B (en) | 2018-11-05 | 2018-11-05 | Soil extracellular enzyme detection device |
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| CN110423678A CN110423678A (en) | 2019-11-08 |
| CN110423678B true CN110423678B (en) | 2022-12-06 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1444014A (en) * | 1973-05-23 | 1976-07-28 | Trentham Ltd G Percy | Soil sampling method and apparatus |
| CN101586145A (en) * | 2008-05-23 | 2009-11-25 | 中国科学院沈阳应用生态研究所 | Analyzing method for detecting activity of soil xylanase |
| CN102606481A (en) * | 2011-01-25 | 2012-07-25 | 广东美芝制冷设备有限公司 | Anti-overheating device for rotary compressor |
| CN204832186U (en) * | 2015-08-20 | 2015-12-02 | 中国农业科学院农业资源与农业区划研究所 | Soil aggregate screening machine |
| CN105938136B (en) * | 2016-05-27 | 2018-08-31 | 山东汇邦渤海农业开发有限公司 | One kind of multiple multi-direction detectors of remains of pesticide data |
| CN108693149A (en) * | 2017-04-06 | 2018-10-23 | 中国科学院地理科学与资源研究所 | A kind of device and method for measuring field rhizosphere soil original position zymogram |
| CN107588978A (en) * | 2017-11-02 | 2018-01-16 | 杭州星翼进出口有限公司 | One kind is based on ground quality detection soil collecting device |
| CN207763983U (en) * | 2018-02-09 | 2018-08-24 | 武瑞京 | A kind of geology soil highly effective gathering classified collection device |
| CN108548688B (en) * | 2018-05-03 | 2019-11-05 | 安徽省通源环境节能股份有限公司 | A kind of soil environment acquisition extract equipment and its soil extract method |
| CN108627382B (en) * | 2018-05-25 | 2020-06-23 | 西南大学 | Sample pretreatment and detection integrated device for determining various indexes of soil based on microfluidic chip technology |
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