CN110420009B - Visual target testing system aiming at different photoreceptor cell dynamic visual stimulation - Google Patents

Visual target testing system aiming at different photoreceptor cell dynamic visual stimulation Download PDF

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CN110420009B
CN110420009B CN201910813000.6A CN201910813000A CN110420009B CN 110420009 B CN110420009 B CN 110420009B CN 201910813000 A CN201910813000 A CN 201910813000A CN 110420009 B CN110420009 B CN 110420009B
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visual target
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target
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CN110420009A (en
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王岳鑫
李学民
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Peking University Third Hospital Peking University Third Clinical Medical College
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B3/00Apparatus for testing the eyes; Instruments for examining the eyes
    • A61B3/02Subjective types, i.e. testing apparatus requiring the active assistance of the patient
    • A61B3/06Subjective types, i.e. testing apparatus requiring the active assistance of the patient for testing light sensitivity, e.g. adaptation; for testing colour vision
    • A61B3/063Subjective types, i.e. testing apparatus requiring the active assistance of the patient for testing light sensitivity, e.g. adaptation; for testing colour vision for testing light sensitivity, i.e. adaptation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B3/00Apparatus for testing the eyes; Instruments for examining the eyes
    • A61B3/02Subjective types, i.e. testing apparatus requiring the active assistance of the patient
    • A61B3/06Subjective types, i.e. testing apparatus requiring the active assistance of the patient for testing light sensitivity, e.g. adaptation; for testing colour vision
    • A61B3/066Subjective types, i.e. testing apparatus requiring the active assistance of the patient for testing light sensitivity, e.g. adaptation; for testing colour vision for testing colour vision
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B3/00Apparatus for testing the eyes; Instruments for examining the eyes
    • A61B3/10Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
    • A61B3/11Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for measuring interpupillary distance or diameter of pupils
    • A61B3/112Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for measuring interpupillary distance or diameter of pupils for measuring diameter of pupils
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B3/00Apparatus for testing the eyes; Instruments for examining the eyes
    • A61B3/10Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
    • A61B3/117Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for examining the anterior chamber or the anterior chamber angle, e.g. gonioscopes
    • A61B3/1173Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions for examining the anterior chamber or the anterior chamber angle, e.g. gonioscopes for examining the eye lens

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Abstract

The invention relates to a visual target testing system aiming at dynamic visual stimulation of different photoreceptor cells, which comprises a visual target element generating module, a visual target parameter control module and a visual target presenting module, wherein the visual target element generating module is used for generating visual target elements which are circles with specific colors and sizes; the visual target parameter control module comprises a visual target size control module and a visual target speed control module; the visual target size control module controls the size of the visual target according to the input parameters; the visual target speed control module controls the movement speed of the visual target according to the input parameters; the visual target presenting module is used for presenting a visual target background, a visual target motion mode and a test flow.

Description

Visual target testing system aiming at different photoreceptor cell dynamic visual stimulation
Technical Field
The invention relates to the technical field of medical biological measurement, in particular to a visual target testing system aiming at different dynamic visual stimuli of photoreceptor cells.
Background
The human retina mainly comprises three cellular structures, namely a photoreceptor cell layer, a bipolar cell layer and a ganglion cell layer. In the process of visual signal transmission, various external visual information firstly reaches the photoreceptor cell layer, is modulated into signals with different time frequencies and spatial frequencies in the photoreceptor cell layer, and further reaches the ganglion cell layer through the transmission of the bipolar cell layer to carry out signal integration. There are three main types of photoreceptor cells in humans, cone cells, rods and intrinsic photosensitive retinal ganglion cells (iprgcs). The rod outer segment contains rhodopsin, is sensitive to blue-green light, is mainly used for sensing scotopic vision, and is a photoreceptor highly sensitive to light. The cone cells mainly function in bright environments and can sense fine resolution, spatial resolution and color vision. IpRGC is a third type of photoreceptor cell that contains melanoidin, is most sensitive to light at a wavelength of around 465nm, and plays an important role in pupillary light reflex and rhythm regulation.
Dynamic vision refers to the perception process by which an observer distinguishes self-movement or external relative movement through visual signal input. The motion vision provides us with a great deal of information from the outside environment, which is relevant to our life, such as motion, driving, etc. The conduction of the moving visual signal is different from the conduction of the static visual signal. At the level of retinal ganglion cells, M ganglion cells mainly conduct signals with high temporal frequency and low spatial frequency, while P ganglion cells conduct signals with low temporal frequency and high spatial frequency, so that M ganglion cells have a large relationship with movement vision signal conduction. While different ganglion cells have some variability in their association with different photoreceptor cells. Previous studies show that rod cells project mainly to M ganglion cells and less to P ganglion cells, and cone cells have no selectivity in the projection of M and P cells, so that different photoreceptors may play different roles in the conduction of motor vision, and the design of a motor visual target and a test system for the stimulation of different photoreceptors is required to research the roles of different photoreceptors in the conduction of motor vision signals. However, no similar optotypes and test systems exist.
Disclosure of Invention
In order to solve the problems, the invention provides a visual target testing system aiming at different photoreceptor cell dynamic visual stimuli, which can solve the problem that different photoreceptor cell movement visual signal conduction paths cannot be independently researched. The test system can conveniently, reasonably and effectively stimulate the motion vision conduction paths of different photoreceptor cells, so that the research on the action of different photoreceptor cells in the motion vision signal conduction becomes possible.
The visual target testing system aiming at the dynamic visual stimulation of different photoreceptor cells comprises a visual target element generating module, a visual target parameter control module and a visual target presenting module, wherein the visual target element generating module is used for generating visual target elements which are circles with specific colors and sizes; the visual target parameter control module comprises a visual target size control module and a visual target speed control module; the visual target size control module controls the size of the visual target according to the input parameters; the visual target speed control module controls the movement speed of the visual target according to the input parameters; the visual target presenting module is used for presenting a visual target background, a visual target motion mode and a test flow.
The color of the visual target element is related to the photoreceptor cell aimed by the test, wherein the visual target color of the stimulation cone cell is red RGB (255,0, 0); the optotype color of the stimulated rod cells and iprgcs is blue RGB (0, 255).
The input parameter received by the sighting mark size control module is the visual angle size of the sighting mark required in the test, the unit is degree, the input parameter received by the sighting mark size control module is a positive integer, and the size is from 1 degree to 10 degrees.
The input parameter received by the visual target speed control module is the speed required in the test, the unit is degree/second, the input parameter received by the visual target speed control module is a positive integer, and the size is from 1 to 100 degrees/second.
The visual target background has different colors and brightness according to different cell types, wherein the visual target background for stimulating the cone cells is white RGB (255 ), and the brightness is 15cd/m2(ii) a The visual target background for stimulating rod cells is black (0,0,0), and the brightness is 0.1cd/m2(ii) a The visual target background for IprGC cells was black (0,0,0) and the luminance was 200cd/m2
The visual target movement mode is horizontal movement, only a background is presented on the screen before the movement starts, and after the movement of the visual target starts, the visual target appears from the midpoint of the leftmost side of the screen and moves to the rightmost side at a constant speed at a set lifting speed horizontally and disappears.
The visual target presenting module is further used for setting a test flow, including adding the test flow, and further setting the repeated appearance times and intervals of the visual targets in the test flow, the sizes and the speeds of the visual targets after the test flow is added; wherein the repeated appearance times of the sighting target are in units of times, the input is a positive integer, and the minimum time is 1; the time interval at which the optotypes appear is in seconds, the input is a positive integer, and the minimum is 0 seconds.
The test process of the cone cells comprises the following steps: the test is started after the test is in a light-on state and is adapted for 10 minutes.
The test process of the rod cells comprises the following steps: the tested object is in a light-off state, is provided with an eye shield, adapts in darkness for 20min, and is switched to a test interface after the program is set, and the eye shield is removed to start testing.
The test flow of the ipRGC comprises the following steps: the tested object is in a light-off state, is provided with an eye shield, adapts in darkness for 20min, and is switched to a test interface after the program is set, and the eye shield is removed to start testing.
Compared with the prior art, the test system provided by the invention has the advantages that an inspector can selectively stimulate a specific photoreceptor cell system by using a specific visual target and test conditions, the test system is only a platform, and the test system can be applied to various researches. In the case of matching with other detection devices, such as a visual electrophysiological testing device, a pupil tracking system, anterior segment tomography and the like, the effects of different photoreceptor cells in the movement visual transduction and the differences of electrophysiological signals, pupil changes, crystalline lenses and anterior segment changes in the movement visual signal transduction of different photoreceptor cells can be specifically explored. The test method provides conditions for the field to deeply research the input and output of the movement visual signals, is simple and convenient to operate and easy to learn, and needs fewer instruments and experimental materials.
Drawings
FIG. 1 is a schematic diagram of an initial setup interface of the test system for different dynamic visual stimuli of photoreceptor cells according to the present invention.
FIG. 2 is a schematic view of a test photoreceptor cell selection interface of the test system of the present invention.
FIG. 3 is a schematic diagram of a test initiation interface of the test system according to the present invention.
FIG. 4 is a schematic view of a parameter setting interface of the test system according to the present invention.
FIG. 5 is a schematic view of a cone cell test interface.
FIG. 6 is a schematic view of a rod cell and iprGC test interface.
Detailed Description
The present invention is described in more detail below to assist in understanding the invention.
The visual target testing system aiming at the dynamic visual stimulation of different photoreceptor cells comprises a visual target element generating module, a visual target parameter control module and a visual target presenting module, wherein the visual target element generating module is used for generating visual target elements which are circles with specific colors and sizes; the visual target parameter control module comprises a visual target size control module and a visual target speed control module; the visual target size control module controls the size of the visual target according to the input parameters; the visual target speed control module controls the movement speed of the visual target according to the input parameters; the visual target presenting module is used for presenting a visual target background, a visual target motion mode and a test flow.
The size of the visual target element can be adjusted, and the unit is degree and represents the size of a visual angle.
The color of the visual target element is related to the photoreceptor cell targeted by the test, wherein the cone cells are sensitive to red, so that the visual target color for stimulating the cone cells is red RGB (255,0, 0); rod cells and ipRGC cells are more sensitive to blue, so the optotype color that stimulates rod cells and ipRGC is blue RGB (0, 255).
The input parameter received by the sighting target size control module is the visual angle size of the sighting target required in the test, the unit is degree, the input parameter is a positive integer, and the size is 1-10 degrees. The actual size of the sighting target is calculated according to the distance from the tested person (namely the tested person) to the screen and the input visual angle size.
The input parameters received by the sighting mark speed control module are the speed required in the test, the unit is degree/second, the input parameters are positive integers, and the size is 1-100 degrees/second. The time when the optotype actually moves in the screen is calculated based on the width of the screen and the inputted speed.
The visual target background has different colors and brightness according to different cell types, wherein the cone cells mainly act in bright environment, so that the visual target background stimulating the cone cells is white RGB (255 ) and has brightness of 15cd/m2(ii) a Sight rodThe cells are mainly used for sensing scotopic vision and are photoreceptors highly sensitive to light, so that the visual target background stimulating the rod cells is black (0,0,0) and the brightness is 0.1cd/m2. IpGC cells are mainly associated with light reflex, and the background of the optotype is designed to be black (0,0,0) and the luminance is 200cd/m according to the prior art2
And designing the sighting target motion mode to be horizontal motion according to the test requirement. Only a background is presented on the screen before the movement starts, and after the movement of the sighting target starts, the sighting target appears from the midpoint of the leftmost side of the screen, and moves horizontally to the right at a set lifting speed at a constant speed to the midpoint of the rightmost side of the screen and disappears.
According to the test requirement, the test flow can be set. The test flow can be added, and the times and intervals of repeated appearance of the sighting target in the test flow, and the size and speed of the sighting target can be further set after the test flow is added. Wherein the repeated appearance times of the sighting target are in units of times, the input is a positive integer, and the minimum time is 1; the time interval of the visual target appearance is in seconds, the input is a positive integer and is 0 seconds at minimum, and the rest parameter settings are referred to visual target elements. After the setting is finished, clicking to start switching to a test interface, pressing a space key to formally start testing, and automatically returning to an initial test photoreceptor cell selection interface after the testing is finished.
And (3) testing environment:
the test is carried out in a quiet, closed room. The size of the room is larger than 5 x 5m2And incandescent lamps are used for indoor illumination. The brightness after turning off the lamp is less than 0.01cd/m2The lamp-on brightness is more than 10cd/m2. In the test process, the brightness of the indoor lighting-on state in the test process of the cone cells is 10-30cd/m2. The inside of the room is in a light-off state in the test process of the rod cells and the iPRCG.
The embodiment of the present invention will be described in detail with reference to fig. 2 to 6 by taking a study of pupils of different photoreceptor cells in motion visual signal input as an example.
1. Testing equipment: the test computer was selected as a Macbook air (13 inches, 2019), retinal display screen, and refresh rate 60 HZ. The test screen is Dell, the screen width is 52cm, and the refresh rate is 60 HZ. And measuring the brightness in the test room in the states of turning on and turning off the lamp by using an illuminometer, and confirming that the brightness meets the requirements.
2. The test flow comprises the following steps:
1) the computer and the display screen are opened, the dynamic visual stimulation sighting target test system for different photoreceptor cells is operated, the interface shown in the figure 1 appears, and the test distance and the size of the test screen are input according to the test requirement, wherein the test distance is 100cm in the example, and the size of the test screen is 52 cm. Click to confirm after input. And exiting if the test click is terminated.
2) After the click is determined, the interface shown in fig. 2 appears, one of the cone cells, the rod cells and the ipRGC is selected according to the test requirement, and the click returns if the test distance and the screen size are input again.
3) Selecting one of the cone cells/rod cells/iprgcs, entering an interface shown in figure 3, and if the click is to be selected again, returning; if the test flow which is set before is applied to directly carry out the test, the test can be started by directly clicking. The default test flow is a single group of sighting marks, the size of the sighting mark is 1 degree, the speed is 2 degrees/second, the test is repeated for 10 times, and the interval time is 0 second; if the test flow needs to be changed, the setting is clicked, and the interface shown in the attached figure 4 is entered. If only the current test group is changed, parameter editing can be carried out after clicking the lower right corner for editing, and the size of the sighting target, the movement speed, the repetition times and the interval time can be adjusted according to the experiment requirements. And after editing, clicking for storage, clicking to return to enter the interface shown in the figure 3 after setting is finished, and clicking to enter a test interface after testing is started. And measuring the screen brightness by using an illuminometer to confirm that the screen brightness meets the requirements.
4) According to the test requirement, the tested object is tested according to the flow of the rod cells, the iprgcs and the cone cells. After entering the corner of the test room, the patient sits in front of the screen 100cm away from the screen, and the height of the chair is adjusted so that the patient looks at the center of the screen basically. The eye mask is firstly put on the tested belt, the light in the room is turned off, the brightness of the computer is adjusted to the darkest state, and the light-tight curtain is applied to cover the computer. The test subjects were allowed to acclimate in a dark environment for 20 minutes. After dark adaptation is finished, the tested eye patch is taken down, and the test is performed to look at the visual target appearing in the screen. After the test is ready, the space key is pressed to start, and according to a preset flow, a horizontally moving blue visual target (RGB-0, 255) appears in the screen, the visual angle is 1 degree, the speed is 2 degrees/second, the operation is repeated for 10 times, and the interval time is 0 second, as shown in figure 6. Fig. 6 shows a legend during testing, not indicating specific brightness and size. Other equipment can be externally connected to detect other indexes of the patient according to the test requirement in the test process. According to the embodiment, the pupil change of different photoreceptor cells in the movement visual signal input is researched, and the pupil size is measured in real time by using a high-definition infrared pupil tracking device in the process of being injected with the sighting target.
5) After the test is finished, the interface shown in the attached figure 2 is automatically jumped to, and other test photosensitive cells are continuously selected according to the experiment requirement. According to the research design, an ipRGC scheme is selected, an interface shown in fig. 3 is switched, the test is started by clicking default settings of the test application, and the test can also be started after clicking the settings to adjust the test flow. The same dark adaptation was performed for 20 minutes on the test subjects, and the post-test method was the same as rod cell test, and after pressing the space bar, horizontally moving blue optotypes (RGB-0, 255) appeared on the screen at a viewing angle of 1 °, a speed of 2 °/sec, 10 repetitions at intervals of 0 sec, as shown in fig. 6. Fig. 6 shows a legend during testing, not indicating specific brightness and size. And after the test is finished, automatically jumping to the interface shown in the attached figure 2, selecting the cone cells, switching to the interface shown in the attached figure 3, clicking to start the test, applying default settings to carry out the test, and switching to the interface shown in the attached figure 5 to carry out the test. The indoor light is turned on, the same adaptation is carried out for 10 minutes on the tested object, and the post-test method is the same as the rod cell test. After pressing the space key, a horizontally moving red visual target (RGB-255,0,0) appears on the screen, with a viewing angle of 1 degree, a speed of 2 degrees/second, 10 repetitions and an interval of 0 second, as shown in FIG. 6. Fig. 6 shows a legend during testing, not indicating specific brightness and size. Other equipment can be externally connected to detect other indexes of the patient according to the test requirement in the test process.
The above-described embodiments are merely illustrative of one of the principles and applications of the present invention, and are not intended to be limiting. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (6)

1. A test system for dynamic visual stimulation targets aiming at different photoreceptor cells is characterized by comprising a target element generation module, a target parameter control module and a target presentation module, wherein the target element generation module is used for generating target elements which are circles with specific colors and sizes; the visual target parameter control module comprises a visual target size control module and a visual target speed control module; the visual target size control module controls the size of the visual target according to the input parameters; the visual target speed control module controls the movement speed of the visual target according to the input parameters; the visual target presenting module is used for presenting a visual target background, a visual target motion mode and a test flow;
the color of the visual target element is related to the photoreceptor cell aimed by the test, wherein the visual target color of the stimulation cone cell is red RGB (255,0, 0); the optotype color of the stimulated rod cells and iprgcs is blue RGB (0, 255);
the test process of the cone cells comprises the following steps: the tested object is in a light-on state, and the test is started after the light is adapted for 10 minutes; the test process of the rod cells comprises the following steps: the test is in a light-off state, an eye cover is arranged, dark adaptation is carried out for 20min, and the eye cover is removed to start testing;
the test flow of the ipRGC comprises the following steps: the test piece is in a light-off state, is provided with an eye mask, adapts to dark for 20min, and is taken off to start the test.
2. The system according to claim 1, wherein the input parameter received by the optotype size control module is the size of the viewing angle of the optotype required in the test in degrees, and the input parameter received by the optotype size control module is a positive integer from 1 ° to 10 °.
3. The system according to claim 1, wherein the parameters of the input received by the target speed control module are the speed required in degree/sec, and the parameters of the input received by the target speed control module are positive integers with the size of 1 to 100 degrees/sec.
4. The system as claimed in claim 1, wherein the visual target background has different colors and brightnesses according to different cell types, and the visual target background for stimulating the cone cells is white RGB (255 ) and has a brightness of 15cd/m2(ii) a The visual target background for stimulating rod cells is black (0,0,0), and the brightness is 0.1cd/m2(ii) a The visual target background for IprGC cells was black (0,0,0) and the luminance was 200cd/m2
5. The system for testing the visual acuity stimulation targets for different photoreceptor cell dynamics according to any one of claims 1-4, wherein the movement pattern of the visual acuity targets is a horizontal movement, only a background is presented on the screen before the movement starts, and after the movement of the visual acuity targets starts, the visual acuity targets appear from the leftmost middle point of the screen and disappear after moving to the right at a constant speed to the rightmost middle point of the screen at a set lifting speed.
6. The system according to claim 1, wherein the optotype presenting module is further configured to set a test procedure, including adding a test procedure, and further setting the number and interval of repeated occurrences of optotypes in the test procedure, and the size and speed of optotypes after adding the test procedure; wherein the repeated appearance times of the sighting target are in units of times, the input is a positive integer, and the minimum time is 1; the time interval at which the optotypes appear is in seconds, the input is a positive integer, and the minimum is 0 seconds.
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