CN110408519A - A kind of nucleic acid-extracting apparatus for molecular diagnosis - Google Patents
A kind of nucleic acid-extracting apparatus for molecular diagnosis Download PDFInfo
- Publication number
- CN110408519A CN110408519A CN201910834426.XA CN201910834426A CN110408519A CN 110408519 A CN110408519 A CN 110408519A CN 201910834426 A CN201910834426 A CN 201910834426A CN 110408519 A CN110408519 A CN 110408519A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- rotation
- extracting apparatus
- electromagnetic wand
- screw rod
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The present invention relates to nucleic acid extraction technical fields, specifically disclose a kind of nucleic acid-extracting apparatus for molecular diagnosis, two slides including the adjustable setting of pedestal and front and back lengthwise position on the base, have cup body by clamp assemblies fixed placement on each slide, and in each cup body lower part rotate be provided with its upper surface have several protrusions rotating disk；Multiple electromagnetic wands are uniformly mounted on the bottom surface of electromagnetic wand mounting bracket；The electromagnetic wand mounting bracket is mounted in electromagnetic wand mounting base by installing bolt disassembly formula, and the electromagnetic wand mounting base is mounted on the bottom end of lifting assembly.Nucleic acid-extracting apparatus provided in an embodiment of the present invention is made electromagnetic wand be placed in one in a cup body, is pasted using electromagnetic wand to nucleic acid by lifting assembly, and electromagnetic wand adsorbs the magnetic bead for being stained with nucleic acid, and progress is facilitated to extract work in next step.
Description
Technical field
The present invention relates to nucleic acid extraction technical field, specifically a kind of nucleic acid-extracting apparatus for molecular diagnosis.
Background technique
The common method of nucleic acid extraction has paramagnetic particle method and strong salty method, and wherein paramagnetic particle method receives superparamagnetism with nanotechnology
The surface of rice grain carries out improvement with after surface modification, is prepared into superparamagnetism silica nanometer magnetic bead, which can be microcosmic
It specifically identifies on interface with nucleic acid molecules and efficiently combines, utilize the superparamagnetism of silica nanosphere, In
It, can be from the DNA in blood, animal tissue, food, pathogenic microorganism equal samples under the action of Chaotropic salt and externally-applied magnetic field
It is separated with RNA, can be applicable to clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detection, food peace
The multiple fields such as full inspection survey, molecular biology research.
It can be realized automation extraction since paramagnetic particle method extracts nucleic acid, carry out high-volume operation, and easy to operate, used time
It is short, thus paramagnetic particle method extracts nucleic acid and increasingly receives attention.The device for extracting nucleic acid currently on the market is mostly bulky, price
Costly and time consuming effort, and that structure is complicated is not portable, therefore a kind of core for molecular diagnosis for minimizing inexpensive facilitation
Sour extraction element becomes a technical problem urgently to be solved currently on the market.
Summary of the invention
The purpose of the present invention is to provide a kind of nucleic acid-extracting apparatus for molecular diagnosis, to solve above-mentioned background technique
The problem of middle proposition.
To achieve the above object, the invention provides the following technical scheme:
A kind of nucleic acid-extracting apparatus for molecular diagnosis, including pedestal and the adjustable setting of front and back lengthwise position are described
Two slides on pedestal have a cup body by clamp assemblies fixed placement on each slide, and lower part in each cup body
Rotation is provided with the rotating disk that its upper surface has several protrusions, and is equipped on the bottom plate of the cup body described for driving
Rotate the driving motor of disc spins；
The end upper surface of the pedestal is provided with support column, and the top level frame of support column is equipped with roof supporting, support
Lifting assembly for adjusting electromagnetic wand height in the vertical direction is installed on top plate；
Multiple electromagnetic wands are uniformly mounted on the bottom surface of electromagnetic wand mounting bracket；The electromagnetic wand mounting bracket passes through installation spiral shell
Bolt reassembling type is mounted in electromagnetic wand mounting base, and the electromagnetic wand mounting base is mounted on the bottom end of lifting assembly.
As a further solution of the present invention: the lifting assembly includes the rotation that rotation is erected on the roof supporting
Swivel nut, rotation swivel nut are connected through a screw thread mode and are socketed on support rod；
The lifting assembly further includes the second clockwise and anticlockwise motor for driving the rotation swivel nut to rotate, just according to second
The direction of rotation for inverting motor driven rotation swivel nut, can be realized the adjustment of support rod height in the vertical direction；Further,
The electromagnetic wand mounting base is rotatably connected on the bottom end of the support rod, to realize to electromagnetic wand height in the vertical direction
Adjustment.
As a further solution of the present invention: the limit for limiting support rod rotation is additionally provided on the roof supporting
Component.
As a further solution of the present invention: the top surface of the roof supporting is fixedly installed with L-type support, for driving rotation
The second clockwise and anticlockwise motor for turning swivel nut rotation is fixedly mounted in L-type support, is pacified on the output shaft of second clockwise and anticlockwise motor
Equipped with driving gear, the outer ring of the rotation swivel nut is fixedly installed with the driven tooth set being meshed with the driving gear.
As a further solution of the present invention: the limit assembly includes the fixed limit for being erected at the roof supporting top surface
Position slide bar and sliding up and down is set in the limit plate on the limit slide bar, the top of the support rod and the limit plate
Side rotation connection.
As a further solution of the present invention: rotation is equipped with second and adjusts screw rod in the pedestal, and level sliding is set to institute
State two in pedestal the second rectangle swivel nuts be connected through a screw thread mode be socketed in it is described second adjust screw rod on, two second
Rectangle swivel nut is fixedly connected between two slides respectively；
It is also equipped on the front side outer wall of the pedestal for driving the described second the first positive and negative rotation for adjusting screw rod rotation
Motor adjusts screw rod using access power supply and the first clockwise and anticlockwise motor of starting driving second and rotates, adjusts screw rod according to second
Direction of rotation, two slides can be pushed to move synchronously on the longitudinal direction of front and back, so that different cup bodies is placed in electromagnetic wand
Underface.
As a further solution of the present invention: the upper surface of the pedestal offers guide-track groove, the bottom end peace of the slide
Equipped with the guide pad being slidingly fitted with the guide-track groove.
As a further solution of the present invention: the clamp assemblies include the first adjusting silk that rotation is set in the slide
The external screw thread of bar, the both ends outer ring of the first adjusting screw rod is oppositely oriented；
The clamp assemblies further include two the first rectangle swivel nuts that horizontal sliding is set in the slide, two the first squares
Shape swivel nut is connected through a screw thread mode and is socketed in the described first both ends outer ring for adjusting screw rod respectively；
Two fixture blocks that the clamp assemblies include are respectively and fixedly installed on two the first rectangle swivel nuts；It extends to described
The first adjusting screw rod end outside slide is also fixedly installed with regulation handle, drives first to adjust screw rod by operation regulation handle
Rotation, the direction of rotation for adjusting screw rod according to first, enables to two the first rectangle swivel nuts close to each other or is located remotely from each other,
When two the first rectangle swivel nuts are close to each other, fixture block thereon is driven to be clamped fixation to cup body.
Compared with prior art, nucleic acid-extracting apparatus provided in an embodiment of the present invention makes electromagnetic wand by lifting assembly
Be placed in one in a cup body, nucleic acid pasted using electromagnetic wand, electromagnetic wand will be stained with the magnetic bead absorption of nucleic acid, it is convenient into
Row extracts work in next step, convenient and efficient；Screw rod is adjusted using access power supply and the first clockwise and anticlockwise motor of starting driving second
Rotation, the direction of rotation for adjusting screw rod according to second, can push two slides to move synchronously on the longitudinal direction of front and back, so that
Different cup bodies is placed in the underface of electromagnetic wand, so that the nucleic acid-extracting apparatus is using more flexible.
Detailed description of the invention
It to describe the technical solutions in the embodiments of the present invention more clearly, below will be to embodiment or description of the prior art
Needed in attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only of the invention some
Embodiment.
Fig. 1 is the structural schematic diagram of the nucleic acid-extracting apparatus provided in an embodiment of the present invention for molecular diagnosis.
Fig. 2 is the enlarged structure schematic diagram of part A in Fig. 1.
Fig. 3 is the structure of Level tune component in the nucleic acid-extracting apparatus provided in an embodiment of the present invention for molecular diagnosis
Schematic diagram.
In figure: 1- pedestal, 2- support column, 3- cup body, 4- roof supporting, 5- electromagnetic wand mounting base, 6- slide, 7- first is just
Invert motor, 8- electromagnetic wand, 9- fixture block, 10- regulation handle；
11- the first rectangle swivel nut, 12- guide pad, 13- guide-track groove, 14- first adjust screw rod, 15- protrusion, 16- rotation
Disk, 17- driving motor, 18- electromagnetic wand mounting bracket, 19- install bolt；
20- limit assembly, 201- limit slide bar, 202- limit plate；
21- studdle, 22- rotate swivel nut, 23- driven tooth set, 24- the second rectangle swivel nut, 25- driving gear, 26-L
Type bracket, the second clockwise and anticlockwise motor of 27-, 28- second adjust screw rod.
Specific embodiment
In order to which technical problems, technical solutions and advantages to be solved are more clearly understood, tie below
Accompanying drawings and embodiments are closed, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only
To explain the present invention, it is not intended to limit the present invention.
Embodiment 1
As shown in Figure 1-3, in one embodiment provided by the invention, a kind of nucleic acid extraction dress for molecular diagnosis
It sets, it is equal on each slide 6 including adjustable two slides 6 being arranged on the pedestal 1 of pedestal 1 and front and back lengthwise position
Have cup body 3 by clamp assemblies fixed placement, and in each cup body 3 lower part rotate be provided with its upper surface have it is several convex
15 rotating disk 16 is played, and the driving motor 17 for driving the rotating disk 16 to rotate is installed on the bottom plate of the cup body 3；
The end upper surface of the pedestal 1 is provided with support column 2, and the top level frame of support column 2 is equipped with roof supporting 4, roof supporting 4
On lifting assembly for adjusting the height in the vertical direction of electromagnetic wand 8 is installed；Multiple electromagnetic wands 8 are uniformly mounted on electromagnetic wand
The bottom surface of mounting bracket 18；The electromagnetic wand mounting bracket 18 is mounted on electromagnetic wand mounting base 5 by installing 19 reassembling type of bolt
On, the electromagnetic wand mounting base 5 is mounted on the bottom end of lifting assembly；
In embodiment provided by the invention, the lifting assembly includes the rotation that rotation is erected on the roof supporting 4
Turn swivel nut 22, rotation swivel nut 22 is connected through a screw thread mode and is socketed on support rod 21；The lifting assembly further includes for driving
The second clockwise and anticlockwise motor 27 that the rotation swivel nut 22 rotates is moved, is additionally provided on the roof supporting 4 for limiting support rod
The limit assembly 20 of 21 rotations, the direction of rotation of rotation swivel nut 22 is driven according to the second clockwise and anticlockwise motor 27, can be realized support
The adjustment of the height in the vertical direction of bar 21；Further, the electromagnetic wand mounting base 5 is rotatably connected on the support rod 21
Bottom end, to realize the adjustment to the height in the vertical direction of electromagnetic wand 8；
Specifically, the top surface of the roof supporting 4 is fixedly installed with L-type support 26 in embodiment provided by the invention,
The second clockwise and anticlockwise motor 27 for driving rotation swivel nut 22 to rotate is fixedly mounted on L-type support 26, second positive and negative rotation
Driving gear 25 is installed, the outer ring of the rotation swivel nut 22 is fixedly installed with and the driving gear on the output shaft of motor 27
The 25 driven tooth sets 23 being meshed；
In embodiments of the present invention, the limit assembly 20 includes the fixed limit for being erected at 4 top surface of roof supporting
Slide bar 201 and sliding up and down is set in the limit plate 202 on the limit slide bar 201, the top of the support rod 21 and institute
State the side rotation connection of limit plate 202.
Embodiment 2
Please continue to refer to Fig. 1-3, unlike the first embodiment, in embodiment provided by the invention, in the pedestal 1
Rotation is equipped with second and adjusts screw rod 28, and two the second rectangle swivel nuts 24 that level sliding is set in the pedestal 1 pass through screw thread
Connection type is socketed in described second and adjusts on screw rod 28, and two the second rectangle swivel nuts 24 are fixed between two slides 6 respectively
Connection；It is also equipped on the front side outer wall of the pedestal 1 for driving the described second the first positive and negative rotation for adjusting the rotation of screw rod 28
Motor 7 adjusts screw rod 28 using access power supply and the first clockwise and anticlockwise motor 7 of starting driving second and rotates, adjusts according to second
The direction of rotation of screw rod 28 can push two slides 6 to move synchronously on the longitudinal direction of front and back, so that different cup bodies 3 is set
In the underface of electromagnetic wand 8；
Further, the upper surface of the pedestal 1 offers guide-track groove 13, the bottom end of the slide 6 be equipped with it is described
The guide pad 12 that guide-track groove 13 is slidingly fitted.
Please continue to refer to Fig. 1, in embodiment provided by the invention, the clamp assemblies include that rotation is set to the slide
First in 6 adjusts screw rod 14, and the external screw thread of the both ends outer ring of the first adjusting screw rod 14 is oppositely oriented, the clamp assemblies
It further include two the first rectangle swivel nuts 11 that horizontal sliding is set in the slide 6, two the first rectangle swivel nuts 11 pass through spiral shell
Line connection type is socketed in the described first both ends outer ring for adjusting screw rod 14, two fixture blocks 9 that the clamp assemblies include respectively
It is respectively and fixedly installed on two the first rectangle swivel nuts 11；It is also solid that first extended to outside the slide 6 adjusts 14 end of screw rod
Dingan County is equipped with regulation handle 10, drives first to adjust screw rod 14 by operation regulation handle 10 and rotates, adjust screw rod according to first
14 direction of rotation enables to two the first rectangle swivel nuts 11 close to each other or is located remotely from each other, when two the first rectangle spiral shells
Cover 11 it is close to each other when, drive fixture block 9 to be thereon clamped fixation to cup body 3.
Nucleic acid-extracting apparatus provided in an embodiment of the present invention makes electromagnetic wand 5 be placed in one a cup by lifting assembly
In body 3, nucleic acid is pasted using electromagnetic wand 5, electromagnetic wand 5 adsorbs the magnetic bead for being stained with nucleic acid, and progress is facilitated to mention in next step
Work is taken, it is convenient and efficient；It adjusts screw rod 28 using access power supply and the first clockwise and anticlockwise motor 7 of starting driving second to rotate, root
The direction of rotation for adjusting screw rod 28 according to second, can push two slides 6 to move synchronously on the longitudinal direction of front and back, so that different
Cup body 3 be placed in the underface of electromagnetic wand 8 so that the nucleic acid-extracting apparatus use it is more flexible.
In the description of the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection " are answered
It is interpreted broadly, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can be mechanical connect
It connects, is also possible to be electrically connected；It can be directly connected, can also can be in two elements indirectly connected through an intermediary
The connection in portion.For the ordinary skill in the art, the tool of above-mentioned term in the present invention can be understood with concrete condition
Body meaning.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (8)
1. a kind of nucleic acid-extracting apparatus for molecular diagnosis, which is characterized in that can including pedestal (1) and front and back lengthwise position
Two slides (6) being arranged on the pedestal (1) are adjusted, have cup by clamp assemblies fixed placement on each slide (6)
Body (3), and each cup body (3) interior lower part rotates and is provided with the rotating disk (16) that its upper surface has several raised (15),
And the driving motor (17) for driving the rotating disk (16) to rotate is installed on the bottom plate of the cup body (3)；
The end upper surface of the pedestal (1) is provided with support column (2), and the top level frame of support column (2) is equipped with roof supporting
(4), the lifting assembly for adjusting electromagnetic wand (8) height in the vertical direction is installed on roof supporting (4)；
Multiple electromagnetic wands (8) are uniformly mounted on the bottom surface of electromagnetic wand mounting bracket (18)；The electromagnetic wand mounting bracket (18) is logical
It crosses installation bolt (19) reassembling type to be mounted on electromagnetic wand mounting base (5), the electromagnetic wand mounting base (5) is mounted on lifting assembly
Bottom end.
2. the nucleic acid-extracting apparatus according to claim 1 for molecular diagnosis, which is characterized in that the lifting assembly packet
The rotation swivel nut (22) that rotation is erected on roof supporting (4) is included, rotation swivel nut (22) is threadedly coupled socket in support rod (21)
On；
The lifting assembly further includes the second clockwise and anticlockwise motor (27) for driving rotation swivel nut (22) to rotate.
3. the nucleic acid-extracting apparatus according to claim 2 for molecular diagnosis, which is characterized in that the roof supporting
(4) limit assembly (20) for limiting support rod (21) rotation is additionally provided on.
4. the nucleic acid-extracting apparatus according to claim 3 for molecular diagnosis, which is characterized in that the limit assembly
It (20) include fixing the limit slide bar (201) for being erected at the roof supporting (4) top surface and sliding up and down to be set in the limit
Limit plate (202) on position slide bar (201), the top of the support rod (21) and the side of the limit plate (202) rotate and connect
It connects.
5. the nucleic acid-extracting apparatus according to claim 3 or 4 for molecular diagnosis, which is characterized in that the branch support
The top surface of plate (4) is fixedly installed with L-type support (26), for driving the second clockwise and anticlockwise motor (27) of rotation swivel nut (22) rotation
It is fixedly mounted on L-type support (26), driving gear (25), institute is installed on the output shaft of second clockwise and anticlockwise motor (27)
The outer ring for stating rotation swivel nut (22) is fixedly installed with the driven tooth set (23) being meshed with the driving gear (25).
6. the nucleic acid-extracting apparatus according to claim 1 to 4 for molecular diagnosis, which is characterized in that the pedestal
(1) rotation is equipped with second and adjusts screw rod (28) in, and it is logical that level sliding is set to two the second rectangle swivel nuts (24) in pedestal (1)
Thread connecting mode is crossed to be socketed on the second adjusting screw rod (28), two the second rectangle swivel nuts (24) respectively with two slides (6)
Between be fixedly connected；
It is also equipped on the front side outer wall of pedestal (1) for driving the described second the first positive and negative rotation electricity for adjusting screw rod (28) rotation
Machine (7).
7. the nucleic acid-extracting apparatus according to claim 6 for molecular diagnosis, which is characterized in that the pedestal (1)
Upper surface offers guide-track groove (13), and the bottom end of the slide (6) is equipped with leads with what the guide-track groove (13) was slidingly fitted
To block (12).
8. the nucleic acid-extracting apparatus according to claim 6 for molecular diagnosis, which is characterized in that the clamp assemblies packet
It includes rotation is set in slide (6) first and adjusts screw rod (14), described first adjusts the external screw thread of the both ends outer ring of screw rod (14)
It is oppositely oriented；
The clamp assemblies further include two the first rectangle swivel nuts (11) that horizontal sliding is set in the slide (6), and two the
One rectangle swivel nut (11) is connected through a screw thread mode and is socketed in the described first both ends outer ring for adjusting screw rod (14) respectively；
Two fixture blocks (9) that the clamp assemblies include are respectively and fixedly installed on two the first rectangle swivel nuts (11)；It extends to
First adjusting screw rod (14) end of the slide (6) outside is also fixedly installed with regulation handle (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910834426.XA CN110408519A (en) | 2019-09-04 | 2019-09-04 | A kind of nucleic acid-extracting apparatus for molecular diagnosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910834426.XA CN110408519A (en) | 2019-09-04 | 2019-09-04 | A kind of nucleic acid-extracting apparatus for molecular diagnosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110408519A true CN110408519A (en) | 2019-11-05 |
Family
ID=68370182
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910834426.XA Pending CN110408519A (en) | 2019-09-04 | 2019-09-04 | A kind of nucleic acid-extracting apparatus for molecular diagnosis |
Country Status (1)
| Country | Link |
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| CN (1) | CN110408519A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111527998A (en) * | 2020-06-08 | 2020-08-14 | 苏州艾捷尔斯生物科技有限公司 | Fungus bag perforator |
| CN111789685A (en) * | 2020-08-14 | 2020-10-20 | 苏州隆泽医药技术有限公司 | High-efficient belt cleaning device of medical surgical instruments |
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| US20170275614A1 (en) * | 2015-01-14 | 2017-09-28 | Sanway Gene Tech Inc. | Device for nucleic acid extraction using magnetic bead method |
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