CN110404103A - A kind of diabetes repair nanometer film and preparation method thereof - Google Patents
A kind of diabetes repair nanometer film and preparation method thereof Download PDFInfo
- Publication number
- CN110404103A CN110404103A CN201910659646.3A CN201910659646A CN110404103A CN 110404103 A CN110404103 A CN 110404103A CN 201910659646 A CN201910659646 A CN 201910659646A CN 110404103 A CN110404103 A CN 110404103A
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- China
- Prior art keywords
- nanometer film
- factor
- diabetes
- repair
- rush
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/225—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Abstract
The invention discloses a kind of diabetes to repair nanometer film and preparation method thereof; diabetes reparation nanometer film forms the factor, rush angiogenesis factor, protective layer by macromolecule matrix material, rush epithelium and constitutes; wherein using natural polymer as basis material, the nanometer film with good mechanical properties is obtained by spin-coating method；It will promote the epithelium formation factor, rush angiogenesis factor loads in nanometer film；It uses water-soluble macromolecule as protective layer, the factor is protected.Diabetes of the invention repair nanometer film biological safety it is good, it is easy to use it is comfortable, can cope with that the complex topography surface of a wound, skin adherence effect is good, can promote diabetes wound healing.
Description
Technical field
The present invention relates to the technical field of bio-medical material, refers in particular to a kind of diabetes and repair nanometer film and its system
Preparation Method.
Background technique
The research and development in huge market, lasting demand, soft tissue repair material are always biomedical materials field
Hot spot.
Diabetes are one of severe complication of diabetes, disease incidence 15-20%.Diabetic foot is slow, and
Disability rate is high, brings heavy mental burden and huge economic pressures to personal, family and society, and becoming, which influences China, pushes away
Dynamic medical industry, the huge obstacle built a harmonious society.
Medical adhesive tape adhesion strength is low, poor to complicated surface of a wound adaptability, it is difficult to which it is subsequent to cause that the surface of a wound is completely covered
Bacterium intrusion infection.Surgical thread suture needs medical practitioner to operate, and when use causes Principle of Pain, it is difficult to for repairing for Micro trauma
It is multiple, easily cause infection.Commercial biological glue mainly has fibrin glue, a-cyanoacrylate etc., and Fibrin Glue is aquatic
Object safety is good, but bonding force is general and expensive；Although a-cyanoacrylate curing rate is fast, intensity is high, have
Certain bio-toxicity, and wound healing can be prevented, modulus is in brittleness greatly, it is difficult to is adapted to tissue.
Diabetes surface of a wound pattern is complicated, and the ulceration surface of a wound is difficult to carry out between common moist dressing zygodactyl, and is being used
Since adhesion strength deficiency itself, easily generation dressing part are even completely fallen off in journey, exposed part will lead to blood or tissue
Liquid exudation, and increase bacterium infection risk.The dressing of clinical use is essentially bulk material at present, it is difficult to adaptation diabetes completely
The foot complexity surface of a wound adheres to demand.It proposes nanometer film being used for diabetes reparation, be assigned based on nanometer film dimensional effect flexible
Property, it may be implemented to carry out the complex topography surface of a wound physical adhesion, load can promote epithelium regeneration and the regenerated factor of blood vessel can
It is a kind of completely new recovery scenario to accelerate the healing of diabetes wound.
Summary of the invention
The purpose of the present invention is to overcome the shortcomings of the existing technology and deficiency, and it is good, available to propose a kind of biological safety
In complicated skin wound, the diabetes of diabetes wound healing is promoted to repair nanometer film and preparation method thereof.
To achieve the above object, technical solution provided by the present invention are as follows: a kind of diabetes reparation nanometer film, by following
Group by mass percentage is grouped as:
Further, the macromolecule matrix material is by one or more of polysaccharide, cellulose, collagen, fibroin albumen
Composition.
Further, the rush epithelium forms the factor and deposits one or more of polypeptide derived from peptide as integrin combination growth-promoting
Composition.
Further, the rush angiogenesis factor is by one or more of the protein derived polypeptide of angiogenesis promoting group
At.
Further, the protective layer is made of one or more of polyvinyl alcohol, cellulose family, water-soluble polysaccharide.
The present invention also provides the preparation methods that a kind of above-mentioned diabetes repair nanometer film, comprising the following steps:
1) polymer-based material is dispersed in water or highly polar solvent, obtains Polymer Solution；
2) the resulting Polymer Solution of step 1) is applied into spin coating proceeding, is dried to obtain nanometer film；
3) the epithelium formation factor and rush angiogenesis factor will be promoted and amide is formed by material surface carboxyl and factor amino
The factor is grafted to a nanometer film surface by key, and obtaining, there is rush epithelium to be formed, promote angiogenesis factor nanometer film；
4) water soluble protective layer is made in the nanometer film that step 3) obtains by spin-coating method, obtaining after dry has protection
Layer promotees the nanometer film that epithelium forms, promotees angiogenesis factor；
5) nanometer film that step 4) obtains is removed from micro slide, is cut into required shape and size, encapsulation.
Compared with prior art, the present invention have the following advantages that with the utility model has the advantages that
A kind of good biocompatibility can be obtained through the invention, implement comfortable complexity that is convenient, can be used for diabetes
The pattern surface of a wound promotes the diabetes of affected part Epithelial restitution, revascularization to repair nanometer film, and diabetes wound can be promoted to be cured
It closes, there is biggish actual promotional value.
Specific embodiment
The present invention is further explained in the light of specific embodiments.
Embodiment 1
Diabetes provided by this implementation repair nanometer film, are grouped as by following group by mass percentage:
Wherein, the macromolecule matrix material is by one or more of polysaccharide, cellulose, collagen, fibroin albumen group
At；The rush epithelium formation factor is deposited one or more of polypeptide derived from peptide by integrin combination growth-promoting and is formed；The rush
Angiogenesis factor is made of protein derived one or more of the polypeptide of angiogenesis promoting；The protective layer is by polyethylene
One or more of alcohol, cellulose family, water-soluble polysaccharide composition.
The preferred embodiment of nanometer film preparation is repaired for the above-mentioned diabetes of the present embodiment below, concrete condition is as follows:
Fibroin albumen is dissolved in hexafluoroisopropanol solution, resulting silk fibroin protein solution is added drop-wise on monocrystalline silicon piece,
And in parameter are as follows: rotation speed 6000rpm, the time be 30s under carry out spin coating, be made gross mass be 0.8% it is polymer-based
Body nanometer film；Macromolecule matrix nanometer film after drying is immersed in methanol solution after 10min and is air-dried；By dry macromolecule
Matrix nano film is immersed in 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride/n-hydroxysuccinimide solution
It is taken out after middle 10min；Macromolecule matrix nanometer film is immersed in and promotees the water that epithelium forms, promotees angiogenesis factor containing 0.075%
It is taken out after 2h in solution, the load of realization factor；The polyvinyl alcohol water solution that gross mass is 99.05% will be accounted for and be added drop-wise to above bear
In the nanometer film for carrying the factor, and in parameter are as follows: rotation speed 6000rpm, time are to carry out spin coating under 20s, obtain having and protect
Sheath and load promote epithelium and form the factor and promote the macromolecule matrix nanometer film of angiogenesis factor.From load after nanometer film is dried
The removing of object on piece, is cut into required shape and drinks size, encapsulates.
Embodiment 2
Fibroin albumen is configured to aqueous solution by the present embodiment unlike the first embodiment, by resulting silk fibroin protein solution
It is added drop-wise on monocrystalline silicon piece, and in parameter are as follows: rotation speed 6000rpm, time are to carry out spin coating under 30s, and gross mass is made
For 0.8% macromolecule matrix nanometer film；Macromolecule matrix nanometer film after drying is immersed in methanol solution wind after 10min
It is dry；Dry macromolecule matrix nanometer film is immersed in 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride/N- hydroxyl
It is taken out after 10min in base succinimide solution；Macromolecule matrix nanometer film is immersed in and promotees epithelium formation containing 0.075%, promote
It is taken out after 2h in the aqueous solution of angiogenesis factor, the load of realization factor；Polyvinyl alcohol water of the gross mass for 99.05% will be accounted for
Solution is added drop-wise in the nanometer film of the above load factor, and in parameter are as follows: rotation speed 6000rpm, time are to carry out under 20s
Spin coating, obtaining, there is protective layer and load, which to promote epithelium, forms the factor and promotees the macromolecule matrix nanometer film of angiogenesis factor.It will
It is removed from micro slide after nanometer film is dry, is cut into required shape and drinks size, encapsulated.
Embodiment 3
Fibroin albumen is dissolved in formic acid solution by the present embodiment unlike the first embodiment, and resulting fibroin albumen is molten
Drop is added on monocrystalline silicon piece, and in parameter are as follows: rotation speed 6000rpm, time are to carry out spin coating under 30s, and total matter is made
The macromolecule matrix nanometer film that amount is 0.8%；Macromolecule matrix nanometer film after drying is immersed in methanol solution after 10min
It air-dries；Dry macromolecule matrix nanometer film is immersed in 1- (3- dimethylamino-propyl) -3- ethyl-carbodiimide hydrochloride/N-
It is taken out after 10min in HOSu NHS solution；By macromolecule matrix nanometer film be immersed in containing 0.075% promote epithelium formed,
Promote to take out after 2h in the aqueous solution of angiogenesis factor, the load of realization factor；Polyvinyl alcohol of the gross mass for 99.05% will be accounted for
Aqueous solution is added drop-wise in the nanometer film of the above load factor, and in parameter are as follows: rotation speed 6000rpm, the time be 20s under into
Row spin coating, obtaining, there is protective layer and load, which to promote epithelium, forms the factor and promotees the macromolecule matrix nanometer film of angiogenesis factor.
It will be removed from micro slide after nanometer film drying, be cut into required shape and drink size, encapsulate.
Embodiment described above is only the preferred embodiments of the invention, and but not intended to limit the scope of the present invention, therefore
All shapes according to the present invention change made by principle, should all be included within the scope of protection of the present invention.
Claims (6)
1. a kind of diabetes repair nanometer film, which is characterized in that be grouped as by following group by mass percentage:
2. a kind of diabetes according to claim 1 repair nanometer film, it is characterised in that: the macromolecule matrix material
It is made of one or more of polysaccharide, cellulose, collagen, fibroin albumen.
3. diabetes according to claim 1 repair nanometer film, it is characterised in that: the rush epithelium forms the factor by whole
Closing element combines growth-promoting to deposit the composition of one or more of polypeptide derived from peptide.
4. diabetes according to claim 1 repair nanometer film, it is characterised in that: the rush angiogenesis factor is by promoting
One or more of protein derived polypeptide of angiogenesis composition.
5. diabetes according to claim 1 repair nanometer film, it is characterised in that: the protective layer by polyvinyl alcohol,
One or more of cellulose family, water-soluble polysaccharide composition.
6. a kind of preparation method of the reparation nanometer film of diabetes described in claim 1, which comprises the following steps:
1) polymer-based material is dispersed in water or highly polar solvent, obtains Polymer Solution；
2) the resulting Polymer Solution of step 1) is applied into spin coating proceeding, is dried to obtain nanometer film；
3) the epithelium formation factor and rush angiogenesis factor will be promoted and amido bond is formed by material surface carboxyl and factor amino, by
The factor is grafted to a nanometer film surface, and obtaining, there is rush epithelium to be formed, promote angiogenesis factor nanometer film；
4) water soluble protective layer is made in the nanometer film that step 3) obtains by spin-coating method, obtained after dry with protective layer,
Promote the nanometer film that epithelium forms, promotees angiogenesis factor；
5) nanometer film that step 4) obtains is removed from micro slide, is cut into required shape and size, encapsulation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910659646.3A CN110404103A (en) | 2019-07-22 | 2019-07-22 | A kind of diabetes repair nanometer film and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910659646.3A CN110404103A (en) | 2019-07-22 | 2019-07-22 | A kind of diabetes repair nanometer film and preparation method thereof |
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| Publication Number | Publication Date |
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| CN110404103A true CN110404103A (en) | 2019-11-05 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910659646.3A Pending CN110404103A (en) | 2019-07-22 | 2019-07-22 | A kind of diabetes repair nanometer film and preparation method thereof |
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Citations (7)
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|---|---|---|---|---|
| US20140221896A1 (en) * | 2011-07-29 | 2014-08-07 | Aluminaid International, Ag | Aluminum-based bandages to aid in medical healing and methods of use |
| CN106512064A (en) * | 2017-01-12 | 2017-03-22 | 广东泰宝医疗器械技术研究院有限公司 | Skin wound dressing with antimicrobial property and preparation method of skin wound dressing |
| CN106581779A (en) * | 2016-11-16 | 2017-04-26 | 华南理工大学 | Skin burn repair material and preparing method thereof |
| CN106581786A (en) * | 2016-11-22 | 2017-04-26 | 华南理工大学 | Functional patch and preparation method and applications thereof |
| CN107789661A (en) * | 2017-09-26 | 2018-03-13 | 华南理工大学 | A kind of hemostatic and antibacterial nanometer film and preparation method thereof |
| CN107982568A (en) * | 2017-09-30 | 2018-05-04 | 重庆市畜牧科学院 | Degradable biological auxiliary material and its preparation method and application |
| CN108744014A (en) * | 2018-06-14 | 2018-11-06 | 福州大学 | A kind of preparation method and products thereof with slow releasing function antiseptic dressing |
-
2019
- 2019-07-22 CN CN201910659646.3A patent/CN110404103A/en active Pending
Patent Citations (7)
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|---|---|---|---|---|
| US20140221896A1 (en) * | 2011-07-29 | 2014-08-07 | Aluminaid International, Ag | Aluminum-based bandages to aid in medical healing and methods of use |
| CN106581779A (en) * | 2016-11-16 | 2017-04-26 | 华南理工大学 | Skin burn repair material and preparing method thereof |
| CN106581786A (en) * | 2016-11-22 | 2017-04-26 | 华南理工大学 | Functional patch and preparation method and applications thereof |
| CN106512064A (en) * | 2017-01-12 | 2017-03-22 | 广东泰宝医疗器械技术研究院有限公司 | Skin wound dressing with antimicrobial property and preparation method of skin wound dressing |
| CN107789661A (en) * | 2017-09-26 | 2018-03-13 | 华南理工大学 | A kind of hemostatic and antibacterial nanometer film and preparation method thereof |
| CN107982568A (en) * | 2017-09-30 | 2018-05-04 | 重庆市畜牧科学院 | Degradable biological auxiliary material and its preparation method and application |
| CN108744014A (en) * | 2018-06-14 | 2018-11-06 | 福州大学 | A kind of preparation method and products thereof with slow releasing function antiseptic dressing |
Non-Patent Citations (1)
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| 曹烨民主编: "《周围血管病中医小丛书 糖尿病下肢病变中医治疗思路》", 30 September 2015, 北京：中国中医药出版社 * |
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Application publication date: 20191105 |