CN107982568A - Degradable biological auxiliary material and its preparation method and application - Google Patents

Degradable biological auxiliary material and its preparation method and application Download PDF

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Publication number
CN107982568A
CN107982568A CN201710914489.7A CN201710914489A CN107982568A CN 107982568 A CN107982568 A CN 107982568A CN 201710914489 A CN201710914489 A CN 201710914489A CN 107982568 A CN107982568 A CN 107982568A
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fibroin
auxiliary material
degradable biological
antibacterial
preparation
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Inventor
周婵
李晋
吕金凤
王介平
杨金龙
谷山林
王海燕
王小燕
戴茜茜
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Chongqing Academy of Animal Sciences
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Chongqing Academy of Animal Sciences
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/28—Polysaccharides or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/44—Medicaments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/64—Use of materials characterised by their function or physical properties specially adapted to be resorbable inside the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/23—Carbohydrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents
    • A61L2300/414—Growth factors

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  • Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Materials Engineering (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The invention belongs to biological medicine Material Field, and in particular to a kind of degradable biological auxiliary material and its preparation method and application.The degradable biological auxiliary material of the present invention, including antibacterial fibroin membrane, chitosan, fibroblast growth factor and aqueous medium；The antibacterial fibroin membrane is antibiotic-peptide modified fibroin protein film；The sequence of the antibacterial peptide such as SEQ ID NO:Shown in 1.Degradable biological auxiliary material biocompatibility provided by the invention, it is nontoxic, non-stimulated, be not easy the allergic reaction that causes body, it can in vitro or in vivo be degraded and absorbed, without being removed from wound after use, there is lasting sterilization, stop blooding and rapidly promote wound healing.

Description

Degradable biological auxiliary material and its preparation method and application
Technical field
The invention belongs to biological medicine Material Field, and in particular to a kind of degradable biological auxiliary material and preparation method thereof and should With.
Background technology
Body surface open wound relates generally to the damage of the histoorgans such as skin, subcutaneous tissue and muscle.How to promote Healing into body surface open wound wound is one of most common problem in daily life.For the solution of this problem Certainly, clinically there are many schemes in surgery.However, with the development of society, people to the quality requirement of medical treatment ＆ health forever without End.It is substantially all secure for the case through medical institutions' such as hospital's processing, wound healing.It is but lighter for some The wound of shred degree, the attitude of self-heal is typically chosen for reason, the people such as medical expense are saved.So seek simpler Just, the remedy measures that effect is more preferable, cost is lower are eternal developing direction.At present, bioactive substance such as seed cell and The intervention of growth factor etc., is one of forward position focus problem of presently relevant research.
Natural polymer currently used for bio-medical field mainly includes polysaccharide and protein-based etc., and wherein shell gathers Sugar, fibroin albumen etc. are most widely used in wound repair field.Nearly ten years, fibroin albumen obtained in terms of purifying significantly into Work(, has been widely applied to biomaterial, they not only have good intensity, but also show splendid biocompatibility. Therefore, the biomaterial based on fibroin albumen and chitosan is applied to wound repair and the theoretical foundation of artificial skin has obtained Confirm, possess good prospect, by as one of best stent of following culture artificial skin.Fibroin albumen, is from silk The natural polymer fibrin of extraction, content account for the 70%-80% of silk, contain 18 kinds of amino acid, wherein glycine (Gly), alanine (Ala) and serine (Ser) account for more than the 80% of total composition.Fibroin has good mechanical performance in itself And physicochemical property, such as good pliability and tensile strength, breathable moisture permeability, slow release, and can by different disposal By obtain it is different in the form of, such as fiber, solution, powder, film and gel.
The medical dressing bioactivity retention time is shorter at present, so how persistently antibacterial and rapidly promote wound exploitation is a kind of The degradable biological auxiliary material of mouth healing has critically important clinical meaning.
The content of the invention
In view of this, it is an object of the invention to provide a kind of degradable biological auxiliary material and its preparation method and application, sheet The degradable biological auxiliary material of invention is non-toxic, non-stimulated, good biocompatibility, has lasting sterilization, stops blooding and rapidly promote The effect of wound healing.
To achieve the above object, the technical scheme is that：
A kind of degradable biological auxiliary material, the composition include antibacterial fibroin membrane, chitosan, fibroblast growth factor And aqueous medium；The antibacterial fibroin membrane is antibiotic-peptide modified fibroin protein film；The sequence of the antibacterial peptide such as SEQ ID NO:1 It is shown.
The sequence of the antibacterial peptide is terminus amidated sequence.Amino acid sequence is：
Leu-Leu-Gly-Asp-Phe-Phe-Arg-Lys-Ser-Lys-Glu-Lys-Ile-Gly-Lys-Glu-Phe- Lys-Arg-Ile-Val-Gln-Arg-Ile-Lys-Asp-Phe-Leu-Arg-AsnLeu-Val-Pro-Arg-Thr-Glu- Ser-NH2。
The antibacterial peptide is LL-37, and LL-37 is the unique a kind of Cathelicidin classes antibacterial peptide found in human body, LL- 37 in addition to direct bactericidal effect, moreover it is possible to adjusts immune and inflammatory mediator release, causes immune cell differentiation and chemotactic, wound Healing, cell factor and histidine release, neutralize endotoxin and the effect such as antitumor.The direct bactericidal effect of LL-37 is not Its most important immunization ways, rear several effects are only the main path that LL-37 participates in immune response.
The antibacterial fibroin membrane obtained by antibiotic-peptide modified fibroin protein film has lasting sterilization and promotes the work(of healing Energy.
Chitosan (CTS) is the unique cationic polysaccharide containing amino in nature, can attract negatively charged blood platelet And red blood cell, due to the hydrophily of amino, by increasing capacitance it is possible to increase the absorption quantity of fibrinogen, so that increase platelet adhesion reaction, shell Glycan, which has, promotes regeneration, antibacterial characteristic, nontoxic, biodegradable, the good compatibility with biological tissue.
During human body wound healing, fibroblasts to secrete collagen is the basic reason of wound skin healing.Institute With every promotion skin fibroblasts growth and secretory factor are alternative favorable factor.Fibroblast gives birth to The long factor (FGF) not only can be strong the fibroblastic growth of promotion and secretion, meanwhile, also have to tissues such as muscle Very strong rush injury repair function.At present, FGF has obtained I kind new medicine certification of China, this is just it in the wide of biomedicine field Road has been paved in general application.
The degradable biological auxiliary material of the present invention is non-toxic, non-stimulated, good biocompatibility, has lasting sterilization, hemostasis The effect of with wound healing is rapidly promoted.
Further, the mass ratio of the antibacterial fibroin membrane and chitosan is 2~5:1~2.
Further, the concentration of the fibroblast growth factor is 0.005-0.02ug/ml.
The second object of the present invention is that providing a kind of degradable biological auxiliary material is preparing for promoting wound to be cured Application in the degradable biological auxiliary material of conjunction.
The third object of the present invention is to provide a kind of preparation method of the degradable biological auxiliary material, including following step Suddenly：
1) after taking silk fibroin protein solution drying process, fibroin protein film is obtained, by fibroin protein film after ethanol solution is handled, Obtain fibroin not soluble in water；
2) fibroin for obtaining step 1) after overactivation be dissolved in PBS buffer the antibacterial peptide reaction 1.5~ 3h, after having reacted, removes unreacted antibacterial peptide with deionized water, obtains antibacterial fibroin membrane；
3) chitosan and fibroblast growth factor are dissolved in aqueous medium, obtain mixed solution；
4) the antibacterial fibroin membrane that step 2) obtains is uniformly mixed with the mixed solution that step 3) obtains, obtains degradable biological Auxiliary material.
Further, ethanol solution is ethanol solution that fraction is 50~100% in step 1).
Further, the mass ratio of fibroin protein film described in step 2) and the step 2) antibacterial peptide is 10~25：0.1.
Further, in step 2) fibroin pass through EDC and NHS mixed solution activation process.
N-hydroxysuccinimide (NHS) is used for synthesizing amino acid protective agent, semi-synthetic kanamycins and medicine intermediate, Often it is used in conjunction with carbodiimides as crosslinking agent.1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides (EDC) are Water-soluble carbodiimide, the activating reagent of carboxyl is used as in acid amides synthesis, is also used for activated phosphate group, protein Crosslinking with nucleic acid and producing for coupled thing is immunized.
Further, PBS buffer is Na in step 2)2HPO4With the mixed solution of NaCl, the pH value of PBS buffer is 6.5。
The degradable biological auxiliary material being prepared the present invention also aims to provide a kind of preparation method.
The beneficial effects of the present invention are：
1) the antibacterial fibroin membrane in degradable biological auxiliary material provided by the invention is obtained by antibiotic-peptide modified fibroin protein film The antibacterial fibroin membrane arrived has the function of lasting sterilization and promotes healing.
2) degradable biological auxiliary material biocompatibility provided by the invention is nontoxic, non-stimulated, be not easy the allergy that causes body Reaction, can be degraded and absorbed in vitro or in vivo, without being removed from wound after use, have lasting sterilization, hemostasis and quick The effect of promoting wound healing.
3) degradable biological auxiliary material can be done directly on general bleeding wounds, Hemorrhage in Deep wound and surgical procedure and be difficult to reach The position bleeding wounds arrived, effectively can play the role of wound protection and buffering external force, easy to use.
Embodiment
It detailed description of a preferred embodiment of the present invention will be given below.The reality of actual conditions is not specified in preferred embodiment Proved recipe method, usually according to normal condition, illustrated embodiment is to preferably be illustrated to present disclosure, but is not Present disclosure is only limitted to illustrated embodiment.So those skilled in the art according to foregoing invention content to embodiment party Case carries out nonessential modifications and adaptations, still falls within protection scope of the present invention.
The preparation method of 1 degradable biological auxiliary material of embodiment
The preparation method of the degradable biological auxiliary material, comprises the following steps：
1) fibroin albumen is dissolved in LiBr aqueous solutions, resulting solution is dialysed into 1~4d to remove in distilled water LiBr, is made silk fibroin water solution, then by silk fibroin solution drying and forming-film, film thickness is about 20~25 μm, by dried silk Plain film soaks each 5min in the ethanol solution of different volumes fraction (100%, 80%, 60%) successively, fibroin membrane is formed not It is dissolved in the fibroin of water；
2) by gained fibroin in PBS buffer (0.1mol/L NaHPO4, 0.5mol/L NaC1, pH=6.5) and immersion 30~45min, then in room temperature in EDCHC1/NHS solution (PBS buffer of 0.5mg/mL EDC, 0.7mg/mL NHS) 15min is soaked, after EDC/NHS is activated, after fibroin membrane is rinsed with PBS buffer, with the antibacterial peptide being dissolved in PBS buffer In room temperature reaction 2h, after the completion of reaction, fibroin membrane is soaked into 5min in PBS buffer, is rinsed 2 times with PBS buffer, then use Deionized water rinsing 1 time is to remove unnecessary antibacterial peptide and other materials, through drying, you can obtain antibacterial fibroin membrane.
3) chitosan and fibroblast growth factor are dissolved in aqueous medium, obtain mixed solution；
4) the antibacterial fibroin membrane that step 2) obtains is uniformly mixed with the mixed solution that step 3) obtains, obtains degradable biological Auxiliary material.
Comparative example 1
As control, by the fibroin membrane through Ethanol Treatment and without EDC/NHS activation in polypeptide solution (0.1mg/mL) Impregnate 2h, antibacterial peptide dipping fibroin membrane be made after cleaning and drying, after again carry out step 3,4 operate to obtain auxiliary material.
Comparative example 2
As control, non-shell adding glycan in degradable biological auxiliary material, remaining preparation method is same as Example 1, obtains auxiliary material.
The Contrast on effect of 2 degradable biological auxiliary material of embodiment
The degradable biological auxiliary material and comparative example 1 prepared using the embodiment of the present invention 1, auxiliary material prepared by comparative example 2 is to wound Bleeding situation, inflammatory reaction, healing effect, tissue morphology feature and the cell proliferative conditions in face are verified that specific method is such as Under：
The healthy rat that weight is 180-220g is chosen, 3% yellow Jackets are injected intraperitoneally at it, are randomly divided into 3 groups. Rat back backbone on both sides of the middle respectively cuts the circular surface of a wound of two a diameter of 1.8cm, and destroys a little musculature, after hemostasis It is spare.With the degradable biological auxiliary material and comparative example 1 for preparing embodiment 1, auxiliary material prepared by comparative example 2 is according to application method It is coated at the surface of a wound, next day dressing.Control group is using physiological saline as negative control.1 day, 3 days, 5 days, 7 days after the surface of a wound is made Photograph to record the observation wound tissue morphological feature such as wound healing situation, HE dyeing and detection cell proliferative conditions.
As a result it is as follows：
Have no that redness is preferred without obvious hematocele hydrops, local skin with the surface of a wound, represent that external application dressing has good biological phase Capacitive, to have any hematocele, hydrops and redness to take second place, biocompatibility is poor, and the embodiment of the present invention 1 is made in the method Standby degradable biological auxiliary material and control auxiliary material using effect is evaluated, the results show that degradation biological prepared by embodiment 1 is auxiliary No inflammation is reacted after 1 day after expecting the processing surface of a wound, auxiliary material prepared by comparative example 2 takes second place, auxiliary material prepared by comparative example 1 ability after 3 days No inflammation is reacted, and degradation biological auxiliary material prepared by the embodiment of the present invention 1 is good compared with control auxiliary material effect.
HE coloration results are complete with surface of a wound epidermal structure, thicker, clear layer, and basal cell is into monolayer alignment, with reference to tight Close, epidermis nail is prominent less, and skin corium collagenous fibres amount is more, and transversely arranged orderly, it is effect that intradermal, which has substantial amounts of angiogenesis, Good, the degradation biological auxiliary material prepared according to HE coloration results to the embodiment of the present invention 1 relatively compares auxiliary material using effect and evaluates, Degradation biological auxiliary material healing effect prepared by embodiment 1 is best, and auxiliary material prepared by comparative example 2 takes second place, auxiliary material prepared by comparative example 1 Healing effect is most slow.
The internal wound effect of 3 degradable biological auxiliary material of embodiment processing
Experimental method：The healthy rat that weight is 180-220g is chosen, 18, male and female dual-purpose, causes femoral artery bleeding to hinder Mouth mold type, sewing-up cut, is randomly divided into 3 groups, every group 9.Respectively with the degradable biological auxiliary material for preparing embodiment 1 and contrast Example 1, auxiliary material prepared by comparative example 2 is coated at the surface of a wound according to application method to be pressed 3 minutes.Observe the general status of each group wound Such as：Bleeding situation, changes of weight, wound local reaction and healing situation.
Experimental result：
Bleeding situation：Degradable biological auxiliary material and comparative example 1 prepared by embodiment 1 is compared with 2 animal sewing-up cut of comparative example Bleeding is few.
Healing state：It is fast compared to degradable biological auxiliary material healing prepared by embodiment 1 with comparative example 1, comparative example 2, more Close all right, comparative example 2 is taken second place, and comparative example 1 heals most slow.
Other situations：After operation in 3 days, animal general status is good, and fur is smooth, and each comparison among groups of weight is without obvious poor It is different.
Experiment conclusion：Degradable biological auxiliary material prepared by embodiment 1 can obviously reduce wound Continuous hemorrhage, wound healing Comparatively fast, also have no adverse effects to the general state of animal, degradable biological auxiliary material of the invention effectively can play wound protection With buffering external force effect, Continuous hemorrhage is prevented, beneficial to wound healing, and there is good security.
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to compared with The present invention is described in detail in good embodiment, it will be understood by those of ordinary skill in the art that, can be to the skill of the present invention Art scheme technical scheme is modified or replaced equivalently, without departing from the objective and scope of technical solution of the present invention, it should all cover at this Among the right of invention.

Claims (10)

1. a kind of degradable biological auxiliary material, it is characterised in that the composition includes antibacterial fibroin membrane, chitosan, into fiber finer The intracellular growth factor and aqueous medium；The antibacterial fibroin membrane is antibiotic-peptide modified fibroin protein film；The sequence of the antibacterial peptide is such as SEQ ID NO:Shown in 1.
2. degradable biological auxiliary material according to claim 1, it is characterised in that the matter of the antibacterial fibroin membrane and chitosan Amount is than being 2~5:1~2.
3. degradable biological auxiliary material according to claim 1, it is characterised in that the fibroblast growth factor it is dense Spend for 0.005-0.02ug/ml.
4. claim 1-3 any one of them degradable biological auxiliary materials are preparing the degradable biological for promoting wound healing Application in auxiliary material.
5. the preparation method of claim 1-3 any one of them degradable biological auxiliary materials, it is characterised in that including following step Suddenly：
1) after taking silk fibroin protein solution drying process, fibroin protein film is obtained, by fibroin protein film after ethanol solution is handled, is obtained not It is dissolved in the fibroin of water；
2) fibroin for obtaining step 1) reacts 1.5~3h after overactivation with the antibacterial peptide being dissolved in PBS buffer, instead After having answered, unreacted antibacterial peptide is removed with deionized water, obtains antibacterial fibroin membrane；
3) chitosan and fibroblast growth factor are dissolved in aqueous medium, obtain mixed solution；
4) the antibacterial fibroin membrane that step 2) obtains is uniformly mixed with the mixed solution that step 3) obtains, it is auxiliary obtains degradable biological Material.
6. preparation method according to claim 5, it is characterised in that in step 1) ethanol solution be fraction be 50~ 100% ethanol solution.
7. preparation method according to claim 5, it is characterised in that fibroin protein film described in step 2) and step 2) institute The mass ratio for stating antibacterial peptide is 10~25：0.1.
8. preparation method according to claim 5, it is characterised in that fibroin is molten by the mixing of EDC and NHS in step 2) Liquid activation process.
9. preparation method according to claim 5, it is characterised in that PBS buffer is Na in step 2)2HPO4And NaCl Mixed solution, the pH value of PBS buffer is 6.5.
10. the degradable biological auxiliary material that the preparation method described in claim 5 is prepared.
CN201710914489.7A 2017-09-30 2017-09-30 Degradable biological auxiliary material and its preparation method and application Pending CN107982568A (en)

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CN110404103A (en) * 2019-07-22 2019-11-05 华南理工大学 A kind of diabetes repair nanometer film and preparation method thereof
CN111317869A (en) * 2020-02-29 2020-06-23 福建医科大学附属第二医院 Novel injectable hydrogel for treating bronchial pleural fistula

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