CN110396481A - A kind of culture medium and its application for ammonia nitrogen degradation bacterium culture - Google Patents
A kind of culture medium and its application for ammonia nitrogen degradation bacterium culture Download PDFInfo
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- CN110396481A CN110396481A CN201810379310.7A CN201810379310A CN110396481A CN 110396481 A CN110396481 A CN 110396481A CN 201810379310 A CN201810379310 A CN 201810379310A CN 110396481 A CN110396481 A CN 110396481A
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- culture medium
- ammonia nitrogen
- culture
- nitrogen degradation
- degradation bacterium
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Abstract
The invention discloses a kind of for cultivating the complex medium of ammonia nitrogen degradation bacterium, it include: ammonium sulfate 1.0-2.0g/L, dipotassium hydrogen phosphate 0.5-1.0g/L, magnesium sulfate 0.2-0.5g/L, sodium chloride 0.5-2.0g/L, ferrous sulfate 0.1-0.4g/L, calcium sulfate 2.0-5.0g/L, sodium dihydrogen phosphate 3.5-5.9g/L, sodium bicarbonate 0.5-1.0g/L, and water, pH value section are 7.8, sterilising temp is 121 DEG C.Have incubation time short with the ammonia nitrogen degradation bacterium of the culture medium culture, the high advantage of degradation rate, 4d ammonia nitrogen degradation rate is up to 89.53%.
Description
Technical field
The present invention relates to a kind of culture mediums for ammonia nitrogen degradation bacterium culture, and its application in sewage treatment.
Background technique
In recent years, as the improvement of people's living standards, making to contain higher ammonia nitrogen in daily sanitary sewage.Life is dirty
Water is drained into the waters such as river, lake, and the ammonia-nitrogen content in water body is caused to improve increasingly, and pollution is got worse, and is caused in water
Fishes and shrimps are poisoned to death, the mass propagation etc. of blue-green alge.And the mass propagation of blue-green alge anaerobic, the release algae poison that will cause water body
The toxicants such as element, and the toxic gases such as stink are discharged, the deterioration of water body has been further resulted in, the just common of people is affected
Water.
The administration way of high ammonia-nitrogen wastewater is divided into physical method, chemical method and three kinds of biological method.Physics and chemistry side
Although method improvement has the advantages that quick, energy consumption is big, and technology controlling and process is difficult and is easy initiation secondary pollution.Biology side
Method, which is administered, has environmental safety high, and ammonia nitrogen removal is high-efficient, the low advantage of the consumption energy.So the removal master of ammonia nitrogen at present
Take the mode of biological removal.The biological removal of broad sense includes that microorganism removes, plant removes, cell free enzyme is biological prosthetic
Deng.It is primarily intended to microorganism removal at present, microorganism removal technology is that either have spy by culture using natural
Distinguished service can microorganism make functional microorganism mass propagation by the culture of control environment, using the metabolism of microorganism come
Reach and converts removal in the microbial body to ammonia nitrogen.
For different ammonia nitrogen degradation microorganisms, need to screen specific culture medium, under the culture medium, ammonia nitrogen degradation is micro-
Biology can be bred rapidly, achieve the purpose that quick, efficient removal ammonia nitrogen.
Summary of the invention
The present invention provides a kind of culture mediums that can stimulate ammonia nitrogen degradation bacterium growth and breeding rapidly.
The strain that the present invention uses is the NBA ammonia nitrogen removal microbial inoculum bought from Guangzhou Bi Wofeng company.
The present invention provides a kind of culture medium for ammonia nitrogen degradation bacterium, the culture medium includes: ammonium sulfate 1.0-2.0g/
L, potassium hydrophosphate 0.5-1.0g/L, magnesium sulfate 0.2-0.5g/Lg/L, sodium chloride 0.5-2.0g/L, ferrous sulfate 0.1-0.4g/
L, calcium sulfate 2.0-5.0g/L, dibastic sodium phosphate salt 3.5-5.9g/L, sodium bicarbonate 0.5-1.0g/L and water.Culture medium of the present invention
Used in solvent be water.
A kind of embodiment according to the present invention, the pH of the culture medium are 7.0-8.0；Further, the culture medium
PH be 7.5-8.0；Further, the pH value of the culture medium is 7.8.In general, can be adjusted using 1MNaOH and 1MHCL
To the range, the too high or too low normal growth that can all influence microorganism of the pH of culture medium shifts to an earlier date pH value so as to cause microorganism
Into decline phase.
A kind of embodiment according to the present invention, the sterilising temp of the culture medium are 121 DEG C, sterilization time 20min.
In above-mentioned culture medium, the potassium hydrophosphate is selected from one or both of potassium dihydrogen phosphate and dipotassium hydrogen phosphate.
In above-mentioned culture medium, the dibastic sodium phosphate salt is selected from one or both of sodium dihydrogen phosphate and disodium hydrogen phosphate.
The addition of dibastic sodium phosphate salt (such as sodium dihydrogen phosphate) and sodium bicarbonate in this complex medium, with potassium hydrophosphate shape
At synergistic effect, there is certain buffer function to the stabilization of pH in culture medium jointly, stable life is provided for ammonia nitrogen degradation bacterium
Long environment.
In the present invention, the amount of dibastic sodium phosphate salt is 3.5-5.9g/L, and the content of dibastic sodium phosphate salt, which continues growing, drops ammonia nitrogen
The growth effect for solving bacterium is little, but its content is too low, is unfavorable for the growth of ammonia nitrogen degradation bacterium.
In the present invention, the culture medium of carbonaceous sources (i.e. sodium bicarbonate) is not unfavorable for the growth of ammonia nitrogen degradation bacterium, proper amount of carbon source
Presence shorten laundering period of function bacterium, improve ammonia nitrogen degradation rate.But ammonia nitrogen drops in the too high levels of sodium bicarbonate
The growth for solving bacterium is inhibited, is also unfavorable for the culture of ammonia nitrogen degradation bacterium.
The present invention also provides a kind of applications for the culture medium of ammonia nitrogen degradation bacterium in sewage treatment as described above.
Complex medium of the invention has raw material sources wide, and environmental pollution is small, at low cost, convenient for storage and transport
The advantages of.Have the ammonia nitrogen degradation bacterium speed of growth fast with the culture medium culture ammonia nitrogen degradation bacterium that the formula is prepared, NH_3-N treating speed
The advantage that degree is fast, degradation rate is high.So technical solution of the present invention can produce higher social and economic benefit, and convenient for promoting
And application.
Detailed description of the invention
Fig. 1 is ammonia nitrogen change curve in the embodiment of the present invention one；
Fig. 2 is ammonia nitrogen change curve in the embodiment of the present invention two；
Fig. 3 is ammonia nitrogen change curve in the embodiment of the present invention three；
Fig. 4 is ammonia nitrogen change curve in the embodiment of the present invention four；
Fig. 5 is ammonia nitrogen change curve in the embodiment of the present invention five；
Fig. 6 is ammonia nitrogen change curve in the embodiment of the present invention six；
Fig. 7 is ammonia nitrogen change curve in the embodiment of the present invention seven；
Fig. 8 is ammonia nitrogen change curve in the embodiment of the present invention eight；
Fig. 9 is ammonia nitrogen change curve in the embodiment of the present invention nine；
Figure 10 is ammonia nitrogen change curve in the embodiment of the present invention ten；
Figure 11 is ammonia nitrogen change curve in the embodiment of the present invention 11；
Figure 12 is ammonia nitrogen change curve in the embodiment of the present invention 12.
Specific embodiment
In order to keep technical solution of the present invention more obvious and easy to understand, it is described in detail below in conjunction with specific embodiment.
Embodiment one
Culture medium prescription are as follows: ammonium sulfate 1.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.2g/L, sodium chloride 0.5g/L,
Ferrous sulfate 0.4g/L, calcium sulfate 2.0g/L, sodium dihydrogen phosphate 5.9g/L, sodium bicarbonate 1.0g/L, tap water configure, and use
It is 7.8 that 1M NaOH and 1M HCL, which adjust pH, and sterilising temp is 121 DEG C, sterilization time 20min.The culture medium to have sterilized is existed
Room temperature is naturally cooled under aseptic condition；Function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, is cultivated
37 DEG C of temperature, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water that detection is produced using Hash company
Quality detection instrument DR6000 is detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.It is real
After testing the results show that cultivating 2d, ammonia nitrogen degradation rate is 28.05%, and after cultivating 4d, ammonia nitrogen concentration reaches minimum, ammonia nitrogen degradation rate
Reach 89.53%.
Embodiment two
Culture medium prescription are as follows: ammonium sulfate 2.0g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, sodium chloride 2.0g/L,
Ferrous sulfate 0.1g/L, calcium sulfate 5.0g/L, sodium dihydrogen phosphate 5.9g/L, sodium bicarbonate 1.0g/L, tap water configure, and use
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilising temp is 121 DEG C, sterilization time 20min.The culture medium to have sterilized is sterile
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.Experiment knot
Fruit shows, after cultivating 2d, ammonia nitrogen degradation rate is 28.05%, and after cultivating 4d, ammonia nitrogen concentration reaches minimum, and ammonia nitrogen degradation rate reaches
84.21%.
Embodiment three
Culture medium prescription be ammonium sulfate 2.0g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, sodium chloride 2.0g/L,
Ferrous sulfate 0.1g/L, calcium sulfate 5.0g/L, sodium dihydrogen phosphate 3.5g/L, sodium bicarbonate 1.0g/L, tap water configure, and use
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilising temp is 121 DEG C, sterilization time 20min.The culture medium to have sterilized is sterile
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change, experiment knot
Fruit shows, after cultivating 2d, ammonia nitrogen degradation rate is 28.05%, after cultivating 4d, after ammonia nitrogen degradation rate reaches 61.40%, 7d, and ammonia nitrogen
Content reaches minimum, and degradation rate reaches 83.33%.
Example IV
Culture medium prescription be ammonium sulfate 2.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.2g/L, sodium chloride 0.5g/L,
Ferrous sulfate 0.4g/L, calcium sulfate 2.0g/L, sodium dihydrogen phosphate 3.5g/L, sodium bicarbonate 0.5g/L, tap water configure, and use
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilising temp is 121 DEG C, sterilization time 20min.The culture medium to have sterilized is sterile
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.Experiment knot
Fruit shows, after cultivating 4d, ammonia nitrogen degradation rate is 73.68%, and after cultivating 7d, ammonia nitrogen degradation rate reaches 82.98%.
Embodiment five
Culture medium prescription is ammonium sulfate 1.0g/L, dipotassium hydrogen phosphate 1.0g/L, magnesium sulfate 0.2g/L, sodium chloride 1.5/L, sulphur
Sour ferrous iron 0.3g/L, calcium sulfate 3.0g/L, sodium dihydrogen phosphate 5.0g/L, sodium bicarbonate 1.0g/L, tap water are configured, are used
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilising temp is 121 DEG C, sterilization time 20min.The culture medium to have sterilized is sterile
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.Experiment knot
Fruit shows that after cultivating 7d, ammonia nitrogen degradation rate reaches 82.46%.
Embodiment six
Culture medium prescription be ammonium sulfate 2.0g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, sodium chloride 2.0g/L,
Ferrous sulfate 0.1g/L, calcium sulfate 5.0g/L, sodium dihydrogen phosphate 5.0g/L, sodium bicarbonate 0.5g/L, tap water configure, and use
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilising temp is 121 DEG C, sterilization time 20min.The culture medium to have sterilized is sterile
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.Experiment knot
Fruit shows that after cultivating 4d, ammonia nitrogen degradation rate reaches 82.46%.
Embodiment seven
Culture medium prescription be ammonium sulfate 2.0g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.2g/L, sodium chloride 1.0g/L,
Ferrous sulfate 0.3g/L, calcium sulfate 3.0g/L, sodium dihydrogen phosphate 5.0g/L, sodium bicarbonate 1.0g/L, tap water configure, and use
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilising temp is 121 DEG C, sterilization time 20min.The culture medium to have sterilized is sterile
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.Experiment knot
Fruit shows, after cultivating 2d, ammonia nitrogen degradation rate is 21.10%, and after cultivating 54d, ammonia nitrogen degradation rate reaches 56.14%.
Embodiment eight
Culture medium prescription is ammonium sulfate 1.5g/L, dipotassium hydrogen phosphate 0.7g/L, magnesium sulfate 0.4g/Lg/L, sodium chloride 1.5g/
L, ferrous sulfate 0.4g/L, calcium sulfate 3.5g/L, sodium dihydrogen phosphate 6.5g/L, sodium bicarbonate 0.7g/L tap water configure,
Adjusting pH with 1MNaOH and 1MHCL is 7.8, and sterilization time is 121 DEG C, sterilising temp 20min.By the culture medium to have sterilized without
Room temperature is naturally cooled under the conditions of bacterium, and function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, culture temperature
37 DEG C of degree shakes revolving speed 150r/min, detects the changes of contents of ammonia nitrogen in culture medium, and detection uses the water quality of Hash company production
Detector DR6000 is detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.Experiment
The results show that ammonia-nitrogen content persistently reduces with the extension of incubation time, degradation rate illustrates buffer salt content without significantly improving
Continue to increase it is little to the growth effect of ammonia nitrogen degradation bacterium.
Embodiment nine
Culture medium prescription be ammonium sulfate 1.5g/L, dipotassium hydrogen phosphate 0.8g/L, magnesium sulfate 0.3g/L, sodium chloride 1.0g/L,
Ferrous sulfate 0.3g/L, calcium sulfate 4.0g/L, sodium dihydrogen phosphate 2.0g/L, sodium bicarbonate 0.7g/L tap water configure, and use
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilization time is 121 DEG C, and sterilising temp is that 20min is sterile by the culture medium to have sterilized
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change, experiment knot
Fruit shows, after cultivating 7d, ammonia nitrogen degradation rate is 47.36%, hereafter has increased trend, illustrates that the reduction of buffer salt is unfavorable for ammonia
The growth of nitrogen degradation bacterium, this may be since buffer salt content is reduced in culture medium, and the phase is unable to maintain that in culture medium after incubation
Caused by requirement of the growth of ammonia nitrogen degradation bacterium to environment.
Embodiment ten
Culture medium prescription be ammonium sulfate 1.4g/L, dipotassium hydrogen phosphate 0.6g/L, magnesium sulfate 0.3g/L, sodium chloride 1.0g/L,
Ferrous sulfate 0.2g/L, calcium sulfate 4.0g/L, sodium dihydrogen phosphate 4.0g/L, sodium bicarbonate 1.5g/L tap water configure, and use
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilization time is 121 DEG C, sterilising temp 20min.The culture medium to have sterilized is sterile
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.Experiment knot
Fruit shows that after cultivating 7d, ammonia-nitrogen content is basically unchanged, and degradation rate is about 31.58%, illustrates that excessively high carbon source is added to ammonia nitrogen degradation
The growth of bacterium has inhibiting effect.
Embodiment 11
Culture medium prescription is ammonium sulfate 1.8g/L, dipotassium hydrogen phosphate 0.7g/L, magnesium sulfate 0.3g/Lg/L, sodium chloride 0.5g/
L, ferrous sulfate 0.2g/L, calcium sulfate 4.5g/L, sodium dihydrogen phosphate 4.0g/L, sodium bicarbonate 0g/L tap water configure, and use
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilization time is 121 DEG C, sterilising temp 20min.The culture medium to have sterilized is sterile
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.Experiment knot
Fruit shows that after cultivating 7d, ammonia-nitrogen content is basically unchanged, and ammonia nitrogen degradation rate is about 29.82%, illustrates the missing of carbon source in culture medium
The growth of function bacterium can be seriously affected.
Embodiment 12
Culture medium prescription is ammonium sulfate 1.7g/L, dipotassium hydrogen phosphate 0.8g/L, magnesium sulfate 0.4g/Lg/L, sodium chloride 1.0g/
L, ferrous sulfate 0.2g/L, calcium sulfate 4.5g/L, sodium dihydrogen phosphate 0g/L, sodium bicarbonate 1.0g/L tap water configure, and use
It is 7.8 that 1MNaOH and 1MHCL, which adjusts pH, and sterilising temp is 121 DEG C, sterilization time 20min.The culture medium to have sterilized is sterile
Under the conditions of naturally cool to room temperature, function bacterium is inoculated in culture medium, inoculum concentration 6%, isothermal vibration culture, cultivation temperature
37 DEG C, revolving speed 150r/min is shaken, detects the changes of contents of ammonia nitrogen in culture medium, the water quality inspection that detection is produced using Hash company
Instrument DR6000 is surveyed to be detected.Detection culture 0d, 2d, 4d respectively, after 7d, 10d in culture medium ammonia nitrogen situation of change.As a result it shows
Show, culture 2d ammonia-nitrogen content decreases, hereafter basicly stable constant.
Claims (7)
1. a kind of culture medium for ammonia nitrogen degradation bacterium culture, including ammonium sulfate 1.0-2.0g/L, potassium hydrophosphate 0.5-1.0g/
L, magnesium sulfate 0.2-0.5g/Lg/L, sodium chloride 0.5-2.0g/L, ferrous sulfate 0.1-0.4g/L, calcium sulfate 2.0-5.0g/L, phosphorus
Sour hydrogen sodium salt 3.5-5.9g/L, sodium bicarbonate 0.5-1.0g/L and water.
2. being used for the culture medium of ammonia nitrogen degradation bacterium culture as described in claim 1, which is characterized in that the pH of the culture medium is
7.0-8.0。
3. being used for the culture medium of ammonia nitrogen degradation bacterium culture as claimed in claim 2, which is characterized in that the pH of the culture medium is
7.8。
4. being used for the culture medium of ammonia nitrogen degradation bacterium culture as described in claim 1, which is characterized in that the sterilizing of the culture medium
Temperature is 121 DEG C.
5. being used for the culture medium of ammonia nitrogen degradation bacterium culture as described in claim 1, which is characterized in that the potassium hydrophosphate choosing
From one or both of potassium dihydrogen phosphate and dipotassium hydrogen phosphate.
6. being used for the culture medium of ammonia nitrogen degradation bacterium culture as described in claim 1, which is characterized in that the dibastic sodium phosphate salt choosing
From one or both of sodium dihydrogen phosphate and disodium hydrogen phosphate.
7. the application for the culture medium of ammonia nitrogen degradation bacterium culture in sewage treatment as described in claim 1.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104004690A (en) * | 2014-06-15 | 2014-08-27 | 华中农业大学 | Nitrobacteria and culturing method for nitrobacteria |
| CN105002110A (en) * | 2015-06-25 | 2015-10-28 | 北京致清源环保科技有限公司 | Composite microbial preparation and application of same in treatment of water body with algal bloom |
-
2018
- 2018-04-25 CN CN201810379310.7A patent/CN110396481A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104004690A (en) * | 2014-06-15 | 2014-08-27 | 华中农业大学 | Nitrobacteria and culturing method for nitrobacteria |
| CN105002110A (en) * | 2015-06-25 | 2015-10-28 | 北京致清源环保科技有限公司 | Composite microbial preparation and application of same in treatment of water body with algal bloom |
Non-Patent Citations (2)
| Title |
|---|
| 周义: "电气石对水理化性质和氨氧化细菌活性的影响", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 碧沃丰生物科技（广东）股份有限公司: "山东某酒业废水处理生物增效案例", 《经典案例》 * |
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| CB02 | Change of applicant information |
Address after: Building 1-3, 619 Caifeng Road, Huzhou City, Zhejiang Province, 313000 Applicant after: Euro American new materials (Zhejiang) Co.,Ltd. Address before: 313000 Zhejiang city of Huzhou Province Economic and Technological Development Zone West Road No. 688 Applicant before: OCHEMATE MATERIAL TECHNOLOGIES Co.,Ltd. |
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| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20191101 |