CN110396125B - Application of arabidopsis transcription factor gene PIF3 in insect stress resistance of plants - Google Patents
Application of arabidopsis transcription factor gene PIF3 in insect stress resistance of plants Download PDFInfo
- Publication number
- CN110396125B CN110396125B CN201810378111.4A CN201810378111A CN110396125B CN 110396125 B CN110396125 B CN 110396125B CN 201810378111 A CN201810378111 A CN 201810378111A CN 110396125 B CN110396125 B CN 110396125B
- Authority
- CN
- China
- Prior art keywords
- plant
- protein
- pif3
- plants
- dna molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000196324 Embryophyta Species 0.000 title claims abstract description 114
- 101001125874 Autographa californica nuclear polyhedrosis virus Per os infectivity factor 3 Proteins 0.000 title claims abstract description 39
- 241000238631 Hexapoda Species 0.000 title abstract description 44
- 241000219194 Arabidopsis Species 0.000 title abstract description 21
- 108091023040 Transcription factor Proteins 0.000 title abstract description 7
- 241000254127 Bemisia tabaci Species 0.000 claims abstract description 41
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 37
- 108020004414 DNA Proteins 0.000 claims abstract description 32
- 239000013598 vector Substances 0.000 claims abstract description 25
- 102000053602 DNA Human genes 0.000 claims abstract description 21
- 230000009261 transgenic effect Effects 0.000 claims abstract description 20
- 101710182318 Protein PIF Proteins 0.000 claims abstract description 15
- 230000014509 gene expression Effects 0.000 claims abstract description 14
- 239000000126 substance Substances 0.000 claims abstract description 14
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- 241000219195 Arabidopsis thaliana Species 0.000 claims description 45
- 238000000034 method Methods 0.000 claims description 23
- 241000607479 Yersinia pestis Species 0.000 claims description 20
- 102000004169 proteins and genes Human genes 0.000 claims description 20
- 241001233957 eudicotyledons Species 0.000 claims description 10
- 241000219193 Brassicaceae Species 0.000 claims description 6
- 108091026890 Coding region Proteins 0.000 claims description 3
- 239000000575 pesticide Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 101150107676 PIF3 gene Proteins 0.000 abstract description 17
- 238000002474 experimental method Methods 0.000 abstract description 13
- 238000009395 breeding Methods 0.000 abstract description 5
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 230000001276 controlling effect Effects 0.000 abstract description 4
- 230000001105 regulatory effect Effects 0.000 abstract description 4
- 238000012216 screening Methods 0.000 abstract description 3
- 230000003115 biocidal effect Effects 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 17
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 8
- 101150113153 PIF1 gene Proteins 0.000 description 8
- 101100408281 Schizosaccharomyces pombe (strain 972 / ATCC 24843) pfh1 gene Proteins 0.000 description 8
- 150000001413 amino acids Chemical group 0.000 description 8
- 238000005286 illumination Methods 0.000 description 8
- 241000589158 Agrobacterium Species 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 101150110490 phyB gene Proteins 0.000 description 7
- 238000010276 construction Methods 0.000 description 6
- 230000002018 overexpression Effects 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 101150014906 TPS10 gene Proteins 0.000 description 4
- 101150047567 TPS14 gene Proteins 0.000 description 4
- 101150090716 TPS21 gene Proteins 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 239000013599 cloning vector Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- 238000001262 western blot Methods 0.000 description 4
- 101001125884 Autographa californica nuclear polyhedrosis virus Per os infectivity factor 1 Proteins 0.000 description 3
- 101001093716 Autographa californica nuclear polyhedrosis virus Per os infectivity factor 4 Proteins 0.000 description 3
- 101001093709 Autographa californica nuclear polyhedrosis virus Per os infectivity factor 5 Proteins 0.000 description 3
- 108010027344 Basic Helix-Loop-Helix Transcription Factors Proteins 0.000 description 3
- 102000018720 Basic Helix-Loop-Helix Transcription Factors Human genes 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 101001125842 Homo sapiens ATP-dependent DNA helicase PIF1 Proteins 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000035882 stress Effects 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 102100029325 ATP-dependent DNA helicase PIF1 Human genes 0.000 description 2
- OMDNCNKNEGFOMM-BQBZGAKWSA-N Ala-Met-Gly Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCSC)C(=O)NCC(O)=O OMDNCNKNEGFOMM-BQBZGAKWSA-N 0.000 description 2
- OTCJMMRQBVDQRK-DCAQKATOSA-N Arg-Asp-Leu Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O OTCJMMRQBVDQRK-DCAQKATOSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 241000258937 Hemiptera Species 0.000 description 2
- 108010064851 Plant Proteins Proteins 0.000 description 2
- LNICFEXCAHIJOR-DCAQKATOSA-N Pro-Ser-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O LNICFEXCAHIJOR-DCAQKATOSA-N 0.000 description 2
- KQNDIKOYWZTZIX-FXQIFTODSA-N Ser-Ser-Arg Chemical compound OC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCCNC(N)=N KQNDIKOYWZTZIX-FXQIFTODSA-N 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- AAZOYLQUEQRUMZ-GSSVUCPTSA-N Thr-Thr-Asn Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CC(N)=O AAZOYLQUEQRUMZ-GSSVUCPTSA-N 0.000 description 2
- 102000040945 Transcription factor Human genes 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 108010019832 glycyl-asparaginyl-glycine Proteins 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 2
- 108091008695 photoreceptors Proteins 0.000 description 2
- 235000021118 plant-derived protein Nutrition 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000005871 repellent Substances 0.000 description 2
- 230000002940 repellent Effects 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 2
- 229960001225 rifampicin Drugs 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 108010026333 seryl-proline Proteins 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 229960000268 spectinomycin Drugs 0.000 description 2
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
- -1 terpene compounds Chemical class 0.000 description 2
- 108010087432 terpene synthase Proteins 0.000 description 2
- 235000007586 terpenes Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 101710186708 Agglutinin Proteins 0.000 description 1
- SVBXIUDNTRTKHE-CIUDSAMLSA-N Ala-Arg-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O SVBXIUDNTRTKHE-CIUDSAMLSA-N 0.000 description 1
- NKJBKNVQHBZUIX-ACZMJKKPSA-N Ala-Gln-Asp Chemical compound C[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O NKJBKNVQHBZUIX-ACZMJKKPSA-N 0.000 description 1
- CKLDHDOIYBVUNP-KBIXCLLPSA-N Ala-Ile-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(O)=O)C(O)=O CKLDHDOIYBVUNP-KBIXCLLPSA-N 0.000 description 1
- XKHLBBQNPSOGPI-GUBZILKMSA-N Ala-Val-Met Chemical compound CSCC[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)N XKHLBBQNPSOGPI-GUBZILKMSA-N 0.000 description 1
- 229940122816 Amylase inhibitor Drugs 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241001600408 Aphis gossypii Species 0.000 description 1
- 108700009446 Arabidopsis PIF3 Proteins 0.000 description 1
- 101100136637 Arabidopsis thaliana BHLH72 gene Proteins 0.000 description 1
- 241000239290 Araneae Species 0.000 description 1
- QPOARHANPULOTM-GMOBBJLQSA-N Arg-Asn-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCCN=C(N)N)N QPOARHANPULOTM-GMOBBJLQSA-N 0.000 description 1
- GHNDBBVSWOWYII-LPEHRKFASA-N Arg-Asn-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCCN=C(N)N)N)C(=O)O GHNDBBVSWOWYII-LPEHRKFASA-N 0.000 description 1
- BSYKSCBTTQKOJG-GUBZILKMSA-N Arg-Pro-Ala Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(O)=O BSYKSCBTTQKOJG-GUBZILKMSA-N 0.000 description 1
- KXOPYFNQLVUOAQ-FXQIFTODSA-N Arg-Ser-Ala Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O KXOPYFNQLVUOAQ-FXQIFTODSA-N 0.000 description 1
- UZSQXCMNUPKLCC-FJXKBIBVSA-N Arg-Thr-Gly Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(O)=O UZSQXCMNUPKLCC-FJXKBIBVSA-N 0.000 description 1
- DAPLJWATMAXPPZ-CIUDSAMLSA-N Asn-Asn-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CC(N)=O DAPLJWATMAXPPZ-CIUDSAMLSA-N 0.000 description 1
- KXFCBAHYSLJCCY-ZLUOBGJFSA-N Asn-Asn-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O KXFCBAHYSLJCCY-ZLUOBGJFSA-N 0.000 description 1
- HLTLEIXYIJDFOY-ZLUOBGJFSA-N Asn-Cys-Asn Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(O)=O HLTLEIXYIJDFOY-ZLUOBGJFSA-N 0.000 description 1
- WONGRTVAMHFGBE-WDSKDSINSA-N Asn-Gly-Gln Chemical compound C(CC(=O)N)[C@@H](C(=O)O)NC(=O)CNC(=O)[C@H](CC(=O)N)N WONGRTVAMHFGBE-WDSKDSINSA-N 0.000 description 1
- PHJPKNUWWHRAOC-PEFMBERDSA-N Asn-Ile-Gln Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CC(=O)N)N PHJPKNUWWHRAOC-PEFMBERDSA-N 0.000 description 1
- YVXRYLVELQYAEQ-SRVKXCTJSA-N Asn-Leu-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(=O)N)N YVXRYLVELQYAEQ-SRVKXCTJSA-N 0.000 description 1
- DJIMLSXHXKWADV-CIUDSAMLSA-N Asn-Leu-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(N)=O DJIMLSXHXKWADV-CIUDSAMLSA-N 0.000 description 1
- GIQCDTKOIPUDSG-GARJFASQSA-N Asn-Lys-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCCN)NC(=O)[C@H](CC(=O)N)N)C(=O)O GIQCDTKOIPUDSG-GARJFASQSA-N 0.000 description 1
- YXVAESUIQFDBHN-SRVKXCTJSA-N Asn-Phe-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(O)=O YXVAESUIQFDBHN-SRVKXCTJSA-N 0.000 description 1
- MKJBPDLENBUHQU-CIUDSAMLSA-N Asn-Ser-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O MKJBPDLENBUHQU-CIUDSAMLSA-N 0.000 description 1
- OERMIMJQPQUIPK-FXQIFTODSA-N Asp-Arg-Ala Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(O)=O OERMIMJQPQUIPK-FXQIFTODSA-N 0.000 description 1
- MRQQMVZUHXUPEV-IHRRRGAJSA-N Asp-Arg-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O MRQQMVZUHXUPEV-IHRRRGAJSA-N 0.000 description 1
- VPSHHQXIWLGVDD-ZLUOBGJFSA-N Asp-Asp-Asp Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O VPSHHQXIWLGVDD-ZLUOBGJFSA-N 0.000 description 1
- RYKWOUUZJFSJOH-FXQIFTODSA-N Asp-Gln-Glu Chemical compound C(CC(=O)N)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CC(=O)O)N RYKWOUUZJFSJOH-FXQIFTODSA-N 0.000 description 1
- KIJLEFNHWSXHRU-NUMRIWBASA-N Asp-Gln-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O KIJLEFNHWSXHRU-NUMRIWBASA-N 0.000 description 1
- VILLWIDTHYPSLC-PEFMBERDSA-N Asp-Glu-Ile Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O VILLWIDTHYPSLC-PEFMBERDSA-N 0.000 description 1
- POTCZYQVVNXUIG-BQBZGAKWSA-N Asp-Gly-Pro Chemical compound OC(=O)C[C@H](N)C(=O)NCC(=O)N1CCC[C@H]1C(O)=O POTCZYQVVNXUIG-BQBZGAKWSA-N 0.000 description 1
- TZOZNVLBTAFJRW-UGYAYLCHSA-N Asp-Ile-Asp Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)[C@H](CC(=O)O)N TZOZNVLBTAFJRW-UGYAYLCHSA-N 0.000 description 1
- MYLZFUMPZCPJCJ-NHCYSSNCSA-N Asp-Lys-Val Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O MYLZFUMPZCPJCJ-NHCYSSNCSA-N 0.000 description 1
- LTCKTLYKRMCFOC-KKUMJFAQSA-N Asp-Phe-Leu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(O)=O LTCKTLYKRMCFOC-KKUMJFAQSA-N 0.000 description 1
- BRRPVTUFESPTCP-ACZMJKKPSA-N Asp-Ser-Glu Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCC(O)=O BRRPVTUFESPTCP-ACZMJKKPSA-N 0.000 description 1
- QOJJMJKTMKNFEF-ZKWXMUAHSA-N Asp-Val-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](N)CC(O)=O QOJJMJKTMKNFEF-ZKWXMUAHSA-N 0.000 description 1
- 101001093708 Autographa californica nuclear polyhedrosis virus Per os infectivity factor 6 Proteins 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 241001498622 Cixius wagneri Species 0.000 description 1
- 241001465977 Coccoidea Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- WAJDEKCJRKGRPG-CIUDSAMLSA-N Cys-His-Ser Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CS)N WAJDEKCJRKGRPG-CIUDSAMLSA-N 0.000 description 1
- VPQZSNQICFCCSO-BJDJZHNGSA-N Cys-Leu-Ile Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O VPQZSNQICFCCSO-BJDJZHNGSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001468872 Fumagospora capnodioides Species 0.000 description 1
- WUAYFMZULZDSLB-ACZMJKKPSA-N Gln-Ala-Asn Chemical compound NC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCC(N)=O WUAYFMZULZDSLB-ACZMJKKPSA-N 0.000 description 1
- KWUSGAIFNHQCBY-DCAQKATOSA-N Gln-Arg-Arg Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O KWUSGAIFNHQCBY-DCAQKATOSA-N 0.000 description 1
- PKVWNYGXMNWJSI-CIUDSAMLSA-N Gln-Gln-Gln Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O PKVWNYGXMNWJSI-CIUDSAMLSA-N 0.000 description 1
- VOLVNCMGXWDDQY-LPEHRKFASA-N Gln-Glu-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCC(=O)N)N)C(=O)O VOLVNCMGXWDDQY-LPEHRKFASA-N 0.000 description 1
- FYAULIGIFPPOAA-ZPFDUUQYSA-N Gln-Ile-Met Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCSC)C(O)=O FYAULIGIFPPOAA-ZPFDUUQYSA-N 0.000 description 1
- IHSGESFHTMFHRB-GUBZILKMSA-N Gln-Lys-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCC(N)=O IHSGESFHTMFHRB-GUBZILKMSA-N 0.000 description 1
- DSRVQBZAMPGEKU-AVGNSLFASA-N Gln-Phe-Cys Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CCC(=O)N)N DSRVQBZAMPGEKU-AVGNSLFASA-N 0.000 description 1
- LGWNISYVKDNJRP-FXQIFTODSA-N Gln-Ser-Gln Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(O)=O LGWNISYVKDNJRP-FXQIFTODSA-N 0.000 description 1
- WPJDPEOQUIXXOY-AVGNSLFASA-N Gln-Tyr-Asn Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CCC(=O)N)N)O WPJDPEOQUIXXOY-AVGNSLFASA-N 0.000 description 1
- WATXSTJXNBOHKD-LAEOZQHASA-N Glu-Asp-Val Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O WATXSTJXNBOHKD-LAEOZQHASA-N 0.000 description 1
- XHUCVVHRLNPZSZ-CIUDSAMLSA-N Glu-Gln-Glu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O XHUCVVHRLNPZSZ-CIUDSAMLSA-N 0.000 description 1
- IQACOVZVOMVILH-FXQIFTODSA-N Glu-Glu-Ser Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O IQACOVZVOMVILH-FXQIFTODSA-N 0.000 description 1
- BPLNJYHNAJVLRT-ACZMJKKPSA-N Glu-Ser-Ala Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O BPLNJYHNAJVLRT-ACZMJKKPSA-N 0.000 description 1
- ZALGPUWUVHOGAE-GVXVVHGQSA-N Glu-Val-His Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)NC(=O)[C@H](CCC(=O)O)N ZALGPUWUVHOGAE-GVXVVHGQSA-N 0.000 description 1
- GGEJHJIXRBTJPD-BYPYZUCNSA-N Gly-Asn-Gly Chemical compound NCC(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O GGEJHJIXRBTJPD-BYPYZUCNSA-N 0.000 description 1
- 108010050006 Gly-Asp-Gly-Arg Proteins 0.000 description 1
- XMPXVJIDADUOQB-RCOVLWMOSA-N Gly-Gly-Ile Chemical compound CC[C@H](C)[C@@H](C([O-])=O)NC(=O)CNC(=O)C[NH3+] XMPXVJIDADUOQB-RCOVLWMOSA-N 0.000 description 1
- YWAQATDNEKZFFK-BYPYZUCNSA-N Gly-Gly-Ser Chemical compound NCC(=O)NCC(=O)N[C@@H](CO)C(O)=O YWAQATDNEKZFFK-BYPYZUCNSA-N 0.000 description 1
- YYXJFBMCOUSYSF-RYUDHWBXSA-N Gly-Phe-Gln Chemical compound [H]NCC(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(N)=O)C(O)=O YYXJFBMCOUSYSF-RYUDHWBXSA-N 0.000 description 1
- HJARVELKOSZUEW-YUMQZZPRSA-N Gly-Pro-Gln Chemical compound [H]NCC(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(O)=O HJARVELKOSZUEW-YUMQZZPRSA-N 0.000 description 1
- HAOUOFNNJJLVNS-BQBZGAKWSA-N Gly-Pro-Ser Chemical compound NCC(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O HAOUOFNNJJLVNS-BQBZGAKWSA-N 0.000 description 1
- SOEGEPHNZOISMT-BYPYZUCNSA-N Gly-Ser-Gly Chemical compound NCC(=O)N[C@@H](CO)C(=O)NCC(O)=O SOEGEPHNZOISMT-BYPYZUCNSA-N 0.000 description 1
- WCORRBXVISTKQL-WHFBIAKZSA-N Gly-Ser-Ser Chemical compound NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O WCORRBXVISTKQL-WHFBIAKZSA-N 0.000 description 1
- LCRDMSSAKLTKBU-ZDLURKLDSA-N Gly-Ser-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)CN LCRDMSSAKLTKBU-ZDLURKLDSA-N 0.000 description 1
- NGBGZCUWFVVJKC-IRXDYDNUSA-N Gly-Tyr-Tyr Chemical compound C([C@H](NC(=O)CN)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)C1=CC=C(O)C=C1 NGBGZCUWFVVJKC-IRXDYDNUSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- YOSQCYUFZGPIPC-PBCZWWQYSA-N His-Asp-Thr Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O YOSQCYUFZGPIPC-PBCZWWQYSA-N 0.000 description 1
- SVVULKPWDBIPCO-BZSNNMDCSA-N His-Phe-Leu Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(O)=O SVVULKPWDBIPCO-BZSNNMDCSA-N 0.000 description 1
- YEKYGQZUBCRNGH-DCAQKATOSA-N His-Pro-Ser Chemical compound C1C[C@H](N(C1)C(=O)[C@H](CC2=CN=CN2)N)C(=O)N[C@@H](CO)C(=O)O YEKYGQZUBCRNGH-DCAQKATOSA-N 0.000 description 1
- PZAJPILZRFPYJJ-SRVKXCTJSA-N His-Ser-Leu Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O PZAJPILZRFPYJJ-SRVKXCTJSA-N 0.000 description 1
- 101710146024 Horcolin Proteins 0.000 description 1
- SLQVFYWBGNNOTK-BYULHYEWSA-N Ile-Gly-Asn Chemical compound CC[C@H](C)[C@@H](C(=O)NCC(=O)N[C@@H](CC(=O)N)C(=O)O)N SLQVFYWBGNNOTK-BYULHYEWSA-N 0.000 description 1
- SVZFKLBRCYCIIY-CYDGBPFRSA-N Ile-Pro-Arg Chemical compound CC[C@H](C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(O)=O SVZFKLBRCYCIIY-CYDGBPFRSA-N 0.000 description 1
- RQJUKVXWAKJDBW-SVSWQMSJSA-N Ile-Ser-Thr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N RQJUKVXWAKJDBW-SVSWQMSJSA-N 0.000 description 1
- IBMVEYRWAWIOTN-UHFFFAOYSA-N L-Leucyl-L-Arginyl-L-Proline Natural products CC(C)CC(N)C(=O)NC(CCCN=C(N)N)C(=O)N1CCCC1C(O)=O IBMVEYRWAWIOTN-UHFFFAOYSA-N 0.000 description 1
- HGCNKOLVKRAVHD-UHFFFAOYSA-N L-Met-L-Phe Natural products CSCCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 HGCNKOLVKRAVHD-UHFFFAOYSA-N 0.000 description 1
- SITWEMZOJNKJCH-UHFFFAOYSA-N L-alanine-L-arginine Natural products CC(N)C(=O)NC(C(O)=O)CCCNC(N)=N SITWEMZOJNKJCH-UHFFFAOYSA-N 0.000 description 1
- 239000012880 LB liquid culture medium Substances 0.000 description 1
- 101710189395 Lectin Proteins 0.000 description 1
- 241000880493 Leptailurus serval Species 0.000 description 1
- KKXDHFKZWKLYGB-GUBZILKMSA-N Leu-Asn-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N KKXDHFKZWKLYGB-GUBZILKMSA-N 0.000 description 1
- VIWUBXKCYJGNCL-SRVKXCTJSA-N Leu-Asn-His Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 VIWUBXKCYJGNCL-SRVKXCTJSA-N 0.000 description 1
- ULXYQAJWJGLCNR-YUMQZZPRSA-N Leu-Asp-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(O)=O ULXYQAJWJGLCNR-YUMQZZPRSA-N 0.000 description 1
- QDSKNVXKLPQNOJ-GVXVVHGQSA-N Leu-Gln-Val Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O QDSKNVXKLPQNOJ-GVXVVHGQSA-N 0.000 description 1
- OXRLYTYUXAQTHP-YUMQZZPRSA-N Leu-Gly-Ala Chemical compound [H]N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(O)=O OXRLYTYUXAQTHP-YUMQZZPRSA-N 0.000 description 1
- VGPCJSXPPOQPBK-YUMQZZPRSA-N Leu-Gly-Ser Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O VGPCJSXPPOQPBK-YUMQZZPRSA-N 0.000 description 1
- OMHLATXVNQSALM-FQUUOJAGSA-N Leu-Ile-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CC(C)C)N OMHLATXVNQSALM-FQUUOJAGSA-N 0.000 description 1
- LIINDKYIGYTDLG-PPCPHDFISA-N Leu-Ile-Thr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(O)=O LIINDKYIGYTDLG-PPCPHDFISA-N 0.000 description 1
- BMVFXOQHDQZAQU-DCAQKATOSA-N Leu-Pro-Asp Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)O)C(=O)O)N BMVFXOQHDQZAQU-DCAQKATOSA-N 0.000 description 1
- DPURXCQCHSQPAN-AVGNSLFASA-N Leu-Pro-Pro Chemical compound CC(C)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 DPURXCQCHSQPAN-AVGNSLFASA-N 0.000 description 1
- IDGZVZJLYFTXSL-DCAQKATOSA-N Leu-Ser-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCCN=C(N)N IDGZVZJLYFTXSL-DCAQKATOSA-N 0.000 description 1
- AKVBOOKXVAMKSS-GUBZILKMSA-N Leu-Ser-Gln Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(O)=O AKVBOOKXVAMKSS-GUBZILKMSA-N 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- UWKNTTJNVSYXPC-CIUDSAMLSA-N Lys-Ala-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCCCN UWKNTTJNVSYXPC-CIUDSAMLSA-N 0.000 description 1
- NQCJGQHHYZNUDK-DCAQKATOSA-N Lys-Arg-Ser Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CO)C(O)=O)CCCN=C(N)N NQCJGQHHYZNUDK-DCAQKATOSA-N 0.000 description 1
- KPJJOZUXFOLGMQ-CIUDSAMLSA-N Lys-Asp-Asn Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)N KPJJOZUXFOLGMQ-CIUDSAMLSA-N 0.000 description 1
- AAORVPFVUIHEAB-YUMQZZPRSA-N Lys-Asp-Gly Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(O)=O AAORVPFVUIHEAB-YUMQZZPRSA-N 0.000 description 1
- VSRXPEHZMHSFKU-IUCAKERBSA-N Lys-Gln-Gly Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(O)=O VSRXPEHZMHSFKU-IUCAKERBSA-N 0.000 description 1
- GJJQCBVRWDGLMQ-GUBZILKMSA-N Lys-Glu-Ala Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(O)=O GJJQCBVRWDGLMQ-GUBZILKMSA-N 0.000 description 1
- IMAKMJCBYCSMHM-AVGNSLFASA-N Lys-Glu-Lys Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(O)=O)CCCCN IMAKMJCBYCSMHM-AVGNSLFASA-N 0.000 description 1
- PGLGNCVOWIORQE-SRVKXCTJSA-N Lys-His-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(O)=O PGLGNCVOWIORQE-SRVKXCTJSA-N 0.000 description 1
- SKRGVGLIRUGANF-AVGNSLFASA-N Lys-Leu-Glu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O SKRGVGLIRUGANF-AVGNSLFASA-N 0.000 description 1
- QLFAPXUXEBAWEK-NHCYSSNCSA-N Lys-Val-Asp Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O QLFAPXUXEBAWEK-NHCYSSNCSA-N 0.000 description 1
- 101710179758 Mannose-specific lectin Proteins 0.000 description 1
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
- WXHHTBVYQOSYSL-FXQIFTODSA-N Met-Ala-Ser Chemical compound CSCC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O WXHHTBVYQOSYSL-FXQIFTODSA-N 0.000 description 1
- UROWNMBTQGGTHB-DCAQKATOSA-N Met-Leu-Asp Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O UROWNMBTQGGTHB-DCAQKATOSA-N 0.000 description 1
- YLDSJJOGQNEQJK-AVGNSLFASA-N Met-Pro-Leu Chemical compound CSCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(O)=O YLDSJJOGQNEQJK-AVGNSLFASA-N 0.000 description 1
- SBFPAAPFKZPDCZ-JYJNAYRXSA-N Met-Pro-Tyr Chemical compound [H]N[C@@H](CCSC)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O SBFPAAPFKZPDCZ-JYJNAYRXSA-N 0.000 description 1
- CIIJWIAORKTXAH-FJXKBIBVSA-N Met-Thr-Gly Chemical compound CSCC[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(O)=O CIIJWIAORKTXAH-FJXKBIBVSA-N 0.000 description 1
- YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 1
- SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 101100408299 Oryza sativa subsp. japonica PIL13 gene Proteins 0.000 description 1
- 241000346285 Ostrinia furnacalis Species 0.000 description 1
- 101150048253 PHYA gene Proteins 0.000 description 1
- 101150023963 PIL1 gene Proteins 0.000 description 1
- 241000488583 Panonychus ulmi Species 0.000 description 1
- FPTXMUIBLMGTQH-ONGXEEELSA-N Phe-Ala-Gly Chemical compound OC(=O)CNC(=O)[C@H](C)NC(=O)[C@@H](N)CC1=CC=CC=C1 FPTXMUIBLMGTQH-ONGXEEELSA-N 0.000 description 1
- ULECEJGNDHWSKD-QEJZJMRPSA-N Phe-Ala-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC1=CC=CC=C1 ULECEJGNDHWSKD-QEJZJMRPSA-N 0.000 description 1
- AGYXCMYVTBYGCT-ULQDDVLXSA-N Phe-Arg-Leu Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(O)=O AGYXCMYVTBYGCT-ULQDDVLXSA-N 0.000 description 1
- MFQXSDWKUXTOPZ-DZKIICNBSA-N Phe-Gln-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CC1=CC=CC=C1)N MFQXSDWKUXTOPZ-DZKIICNBSA-N 0.000 description 1
- KJJROSNFBRWPHS-JYJNAYRXSA-N Phe-Glu-Leu Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O KJJROSNFBRWPHS-JYJNAYRXSA-N 0.000 description 1
- ZJPGOXWRFNKIQL-JYJNAYRXSA-N Phe-Pro-Pro Chemical compound C([C@H](N)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(O)=O)C1=CC=CC=C1 ZJPGOXWRFNKIQL-JYJNAYRXSA-N 0.000 description 1
- VIIRRNQMMIHYHQ-XHSDSOJGSA-N Phe-Val-Pro Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CC2=CC=CC=C2)N VIIRRNQMMIHYHQ-XHSDSOJGSA-N 0.000 description 1
- 108020005120 Plant DNA Proteins 0.000 description 1
- 108010089814 Plant Lectins Proteins 0.000 description 1
- 108020005089 Plant RNA Proteins 0.000 description 1
- DZZCICYRSZASNF-FXQIFTODSA-N Pro-Ala-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1 DZZCICYRSZASNF-FXQIFTODSA-N 0.000 description 1
- DIFXZGPHVCIVSQ-CIUDSAMLSA-N Pro-Gln-Ser Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(O)=O DIFXZGPHVCIVSQ-CIUDSAMLSA-N 0.000 description 1
- LXLFEIHKWGHJJB-XUXIUFHCSA-N Pro-Ile-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@@H]1CCCN1 LXLFEIHKWGHJJB-XUXIUFHCSA-N 0.000 description 1
- DRKAXLDECUGLFE-ULQDDVLXSA-N Pro-Leu-Phe Chemical compound CC(C)C[C@H](NC(=O)[C@@H]1CCCN1)C(=O)N[C@@H](Cc1ccccc1)C(O)=O DRKAXLDECUGLFE-ULQDDVLXSA-N 0.000 description 1
- WLJYLAQSUSIQNH-GUBZILKMSA-N Pro-Met-Ser Chemical compound CSCC[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@@H]1CCCN1 WLJYLAQSUSIQNH-GUBZILKMSA-N 0.000 description 1
- GFHOSBYCLACKEK-GUBZILKMSA-N Pro-Pro-Asn Chemical compound [H]N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(O)=O GFHOSBYCLACKEK-GUBZILKMSA-N 0.000 description 1
- SBVPYBFMIGDIDX-SRVKXCTJSA-N Pro-Pro-Pro Chemical compound OC(=O)[C@@H]1CCCN1C(=O)[C@H]1N(C(=O)[C@H]2NCCC2)CCC1 SBVPYBFMIGDIDX-SRVKXCTJSA-N 0.000 description 1
- SEZGGSHLMROBFX-CIUDSAMLSA-N Pro-Ser-Gln Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(O)=O SEZGGSHLMROBFX-CIUDSAMLSA-N 0.000 description 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
- 238000010802 RNA extraction kit Methods 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- JPIDMRXXNMIVKY-VZFHVOOUSA-N Ser-Ala-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O JPIDMRXXNMIVKY-VZFHVOOUSA-N 0.000 description 1
- DBIDZNUXSLXVRG-FXQIFTODSA-N Ser-Asp-Met Chemical compound CSCC[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CO)N DBIDZNUXSLXVRG-FXQIFTODSA-N 0.000 description 1
- BLPYXIXXCFVIIF-FXQIFTODSA-N Ser-Cys-Arg Chemical compound C(C[C@@H](C(=O)O)NC(=O)[C@H](CS)NC(=O)[C@H](CO)N)CN=C(N)N BLPYXIXXCFVIIF-FXQIFTODSA-N 0.000 description 1
- HJEBZBMOTCQYDN-ACZMJKKPSA-N Ser-Glu-Asp Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O HJEBZBMOTCQYDN-ACZMJKKPSA-N 0.000 description 1
- QKQDTEYDEIJPNK-GUBZILKMSA-N Ser-Glu-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CO QKQDTEYDEIJPNK-GUBZILKMSA-N 0.000 description 1
- VQBCMLMPEWPUTB-ACZMJKKPSA-N Ser-Glu-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O VQBCMLMPEWPUTB-ACZMJKKPSA-N 0.000 description 1
- UAJAYRMZGNQILN-BQBZGAKWSA-N Ser-Gly-Met Chemical compound [H]N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CCSC)C(O)=O UAJAYRMZGNQILN-BQBZGAKWSA-N 0.000 description 1
- UIGMAMGZOJVTDN-WHFBIAKZSA-N Ser-Gly-Ser Chemical compound OC[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O UIGMAMGZOJVTDN-WHFBIAKZSA-N 0.000 description 1
- GJFYFGOEWLDQGW-GUBZILKMSA-N Ser-Leu-Gln Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)O)NC(=O)[C@H](CO)N GJFYFGOEWLDQGW-GUBZILKMSA-N 0.000 description 1
- UBRMZSHOOIVJPW-SRVKXCTJSA-N Ser-Leu-Lys Chemical compound OC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(O)=O UBRMZSHOOIVJPW-SRVKXCTJSA-N 0.000 description 1
- IXZHZUGGKLRHJD-DCAQKATOSA-N Ser-Leu-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(O)=O IXZHZUGGKLRHJD-DCAQKATOSA-N 0.000 description 1
- LRZLZIUXQBIWTB-KATARQTJSA-N Ser-Lys-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O LRZLZIUXQBIWTB-KATARQTJSA-N 0.000 description 1
- DINQYZRMXGWWTG-GUBZILKMSA-N Ser-Pro-Pro Chemical compound OC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 DINQYZRMXGWWTG-GUBZILKMSA-N 0.000 description 1
- CKDXFSPMIDSMGV-GUBZILKMSA-N Ser-Pro-Val Chemical compound [H]N[C@@H](CO)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(O)=O CKDXFSPMIDSMGV-GUBZILKMSA-N 0.000 description 1
- PPCZVWHJWJFTFN-ZLUOBGJFSA-N Ser-Ser-Asp Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O PPCZVWHJWJFTFN-ZLUOBGJFSA-N 0.000 description 1
- XQJCEKXQUJQNNK-ZLUOBGJFSA-N Ser-Ser-Ser Chemical compound OC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O XQJCEKXQUJQNNK-ZLUOBGJFSA-N 0.000 description 1
- PYTKULIABVRXSC-BWBBJGPYSA-N Ser-Ser-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PYTKULIABVRXSC-BWBBJGPYSA-N 0.000 description 1
- VGQVAVQWKJLIRM-FXQIFTODSA-N Ser-Ser-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(O)=O VGQVAVQWKJLIRM-FXQIFTODSA-N 0.000 description 1
- PMTWIUBUQRGCSB-FXQIFTODSA-N Ser-Val-Ala Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(O)=O PMTWIUBUQRGCSB-FXQIFTODSA-N 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- KEGBFULVYKYJRD-LFSVMHDDSA-N Thr-Ala-Phe Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 KEGBFULVYKYJRD-LFSVMHDDSA-N 0.000 description 1
- JNQZPAWOPBZGIX-RCWTZXSCSA-N Thr-Arg-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)[C@@H](C)O)CCCN=C(N)N JNQZPAWOPBZGIX-RCWTZXSCSA-N 0.000 description 1
- LXWZOMSOUAMOIA-JIOCBJNQSA-N Thr-Asn-Pro Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N1CCC[C@@H]1C(=O)O)N)O LXWZOMSOUAMOIA-JIOCBJNQSA-N 0.000 description 1
- KGKWKSSSQGGYAU-SUSMZKCASA-N Thr-Gln-Thr Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N)O KGKWKSSSQGGYAU-SUSMZKCASA-N 0.000 description 1
- KRDSCBLRHORMRK-JXUBOQSCSA-N Thr-Lys-Ala Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O KRDSCBLRHORMRK-JXUBOQSCSA-N 0.000 description 1
- GUHLYMZJVXUIPO-RCWTZXSCSA-N Thr-Met-Val Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C(C)C)C(O)=O GUHLYMZJVXUIPO-RCWTZXSCSA-N 0.000 description 1
- BKIOKSLLAAZYTC-KKHAAJSZSA-N Thr-Val-Asn Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O BKIOKSLLAAZYTC-KKHAAJSZSA-N 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- 101100136638 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) PIF7 gene Proteins 0.000 description 1
- ZAGPDPNPWYPEIR-SRVKXCTJSA-N Tyr-Cys-Ser Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(O)=O ZAGPDPNPWYPEIR-SRVKXCTJSA-N 0.000 description 1
- AZGZDDNKFFUDEH-QWRGUYRKSA-N Tyr-Gly-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CC1=CC=C(O)C=C1 AZGZDDNKFFUDEH-QWRGUYRKSA-N 0.000 description 1
- PRONOHBTMLNXCZ-BZSNNMDCSA-N Tyr-Leu-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 PRONOHBTMLNXCZ-BZSNNMDCSA-N 0.000 description 1
- GXAZTLJYINLMJL-LAEOZQHASA-N Val-Asn-Gln Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N GXAZTLJYINLMJL-LAEOZQHASA-N 0.000 description 1
- GNWUWQAVVJQREM-NHCYSSNCSA-N Val-Asn-His Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N GNWUWQAVVJQREM-NHCYSSNCSA-N 0.000 description 1
- VLOYGOZDPGYWFO-LAEOZQHASA-N Val-Asp-Glu Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O VLOYGOZDPGYWFO-LAEOZQHASA-N 0.000 description 1
- UZFNHAXYMICTBU-DZKIICNBSA-N Val-Phe-Gln Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N UZFNHAXYMICTBU-DZKIICNBSA-N 0.000 description 1
- UGFMVXRXULGLNO-XPUUQOCRSA-N Val-Ser-Gly Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CO)C(=O)NCC(O)=O UGFMVXRXULGLNO-XPUUQOCRSA-N 0.000 description 1
- UFCHCOKFAGOQSF-BQFCYCMXSA-N Val-Trp-Glu Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N UFCHCOKFAGOQSF-BQFCYCMXSA-N 0.000 description 1
- ZHWZDZFWBXWPDW-GUBZILKMSA-N Val-Val-Cys Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(O)=O ZHWZDZFWBXWPDW-GUBZILKMSA-N 0.000 description 1
- NLNCNKIVJPEFBC-DLOVCJGASA-N Val-Val-Glu Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CCC(O)=O NLNCNKIVJPEFBC-DLOVCJGASA-N 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 108010005233 alanylglutamic acid Proteins 0.000 description 1
- 108010047495 alanylglycine Proteins 0.000 description 1
- 108010087924 alanylproline Proteins 0.000 description 1
- 239000003392 amylase inhibitor Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 108010062796 arginyllysine Proteins 0.000 description 1
- 108010069205 aspartyl-phenylalanine Proteins 0.000 description 1
- 108010038633 aspartylglutamate Proteins 0.000 description 1
- 108010092854 aspartyllysine Proteins 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 230000004790 biotic stress Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 101150081158 crtB gene Proteins 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 108010016616 cysteinylglycine Proteins 0.000 description 1
- 230000026535 de-etiolation Effects 0.000 description 1
- 230000009683 detection of insect Effects 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 108010063718 gamma-glutamylaspartic acid Proteins 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 238000003209 gene knockout Methods 0.000 description 1
- 238000003208 gene overexpression Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- XBGGUPMXALFZOT-UHFFFAOYSA-N glycyl-L-tyrosine hemihydrate Natural products NCC(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 XBGGUPMXALFZOT-UHFFFAOYSA-N 0.000 description 1
- 108010067216 glycyl-glycyl-glycine Proteins 0.000 description 1
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Natural products NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 108010036413 histidylglycine Proteins 0.000 description 1
- 108010028295 histidylhistidine Proteins 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 108010031424 isoleucyl-prolyl-proline Proteins 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 108010090333 leucyl-lysyl-proline Proteins 0.000 description 1
- 108010073472 leucyl-prolyl-proline Proteins 0.000 description 1
- 108010057821 leucylproline Proteins 0.000 description 1
- 230000004301 light adaptation Effects 0.000 description 1
- 230000004298 light response Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 108010016686 methionyl-alanyl-serine Proteins 0.000 description 1
- 108010068488 methionylphenylalanine Proteins 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000030648 nucleus localization Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 108010074082 phenylalanyl-alanyl-lysine Proteins 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108010087846 prolyl-prolyl-glycine Proteins 0.000 description 1
- 108010031719 prolyl-serine Proteins 0.000 description 1
- 108010090894 prolylleucine Proteins 0.000 description 1
- 108010053725 prolylvaline Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 108010071207 serylmethionine Proteins 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 108010031491 threonyl-lysyl-glutamic acid Proteins 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Pest Control & Pesticides (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Insects & Arthropods (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses application of an arabidopsis transcription factor gene PIF3 in insect stress resistance of plants. The invention provides an application of any one of the following substances 1) -3) in regulating and controlling insect resistance of plants: 1) protein PIF 3; 2) a DNA molecule encoding the protein PIF 3; 3) a recombinant vector, an expression cassette, a transgenic cell line or a recombinant bacterium containing a DNA molecule encoding the protein PIF 3; experiments prove that after the PIF3 gene is overexpressed, arabidopsis shows a phenotype of resisting bemisia tabaci. In order to facilitate the identification and screening of transgenic Arabidopsis plants, the vectors used may be processed, for example, by adding plant selectable markers or antibiotic markers having resistance. The PIF3 gene provided by the invention provides gene resources for breeding new insect-resistant plant varieties and has good potential application value.
Description
Technical Field
The invention belongs to the field of genetic engineering, and particularly relates to application of an arabidopsis transcription factor gene PIF3 in insect stress resistance of plants.
Background
Agricultural insect pests are one of main agricultural disasters in China, and have the characteristics of multiple types, large influence and frequent outbreak of disasters, the occurrence range and the severity of the agricultural insect pests cause great loss to national economy, particularly agricultural production in China, and besides directly endanger crops, the agricultural insect pests carry pathogens or parasites to cause epidemic outbreaks of diseases and double damages of the diseases and the pests to seriously influence the national civilian life. Common agricultural pests in China include whitefly, rice planthopper, corn borer, cotton bollworm, cotton aphid, wheat red spider, locust and the like. In recent years, frequent outbreaks of various crop pests and diseases and aggravation of a series of agricultural problems caused by abuse and unreasonable utilization of chemical fertilizers and pesticides urgently need a more environment-friendly and sustainable method for realizing green plant protection. Improving the insect resistance of the plant itself is the most fundamentally effective method. These current methods include the following: adopting biotechnology to culture resistant varieties, such as stably expressing toxin protein Bt of Bacillus thuringiensis, agglutinin of various plants, etc. for resisting chewing insects; stably expressing dsRNA of genes related to key development, physiology and metabolism of insects; the agricultural means is improved, the cultivation density is controlled, the use of nitrogen fertilizer is controlled, and the robust growth of field crops is ensured; according to the pest damage period of certain pests, the ecological pest resistance of the plant is improved by sowing the pest early or late; controlling population density of pests using natural enemies, and the like. In agricultural pests, piercing-sucking mouthpart pests such as whitefly, aphid, coccid, spider mite, thrips and the like are easy to spread and spread by various media, and most of the piercing-sucking mouthpart pests are carriers and intermediate media of pathogenic microorganisms, but an effective method for resisting the piercing-sucking insects is not found at present.
Bemisia tabaci (Bemisiatabaci) is a vector insect that transmits plant viruses, is an important pest of many crops worldwide, and viruses transmitted by bemisia tabaci are as many as 200, causing significant economic loss on food and economic crops such as wheat, tobacco, tomato, cotton, soybean. The bemisia tabaci not only can transmit plant viruses to cause diseases to host plants, but also can cause great threat to the host plants. The bemisia tabaci directly stabs and absorbs plant juice to cause plant weakness, nymphs and adults can also secrete honeydew to induce the generation of sooty mould, and photosynthesis is seriously influenced. At present, the tobacco whitefly is still prevented and controlled by a chemical insecticide with high toxicity and low selectivity in production, so that the problems of drug resistance, pollution, residue and the like of pests are caused, and the safety of the tongue tip of the common people is seriously influenced. The double hazards of the plant and the vector insect cause great difficulty in effective prevention and control in production, so that the improvement of the resistance level of the plant has important significance for effectively controlling bemisia tabaci and plant virus diseases.
PIFs (phytochrome interacting factors), which are phytochrome interacting factors, are a bHLH family of transcription factors that can interact with photoreceptor photopigment proteins. PIF3 as a transcription factor can act with photoreceptors phyA and phyB, and after seedlings growing in the dark are irradiated, PIF3 in vivo can be rapidly degraded under the control of phytochrome to negatively control a phyB signal pathway. The homologous genes of PIF3, PIF1, PIF4 and PIF5 also play an important role. PIF1 encodes a nuclear bHLH protein that can be induced by light and that can be involved in the photopigment signaling pathway; PIF4 encodes a nuclear localization bHLH protein, which can interact with phyB protein to negatively regulate phyB-mediated red light response pathway and participate in SAS process; PIF5 functions as a phyB negative regulator, with protein levels regulated by phyB. In addition, both the PIF6 and PIL1 genes are members of the PIF3 transcription factor gene family. PIF7 negatively regulates phyB-mediated seedling de-etiolation. The current research on PIFs focuses on the regulation of plant optical signal channels, and reports on the resistance of plants to biotic stress are not reported.
At present, the research of plant molecular biology is mostly carried out by taking Arabidopsis thaliana (Arabidopsis thaliana) as a model plant, and the Arabidopsis thaliana belongs to cruciferae plants, has the advantages of small genome, fast growth and the like, and is widely used for the research of plant genetics, cell biology, molecular biology and the like. Generally, by means of agrobacterium infection or other transgenic methods, a related gene cloning vector can be transferred into wild arabidopsis thaliana to obtain an over-expression or gene knockout strain of the gene, and then insect bioassay experimental detection is performed to research the function of the gene in insect resistance, so that insect-resistant gene resources are discovered. At present, some genes which can significantly improve the insect resistance of plants, such as protease inhibitor genes (PI genes), amylase inhibitor genes, other plant lectin genes and the like, are discovered.
Disclosure of Invention
An object of the present invention is to provide use of any one of the following 1) to 3).
The invention provides an application of any one of the following substances 1) -3) in regulating and controlling insect resistance of plants:
1) protein PIF 3;
2) a DNA molecule encoding the protein PIF 3;
3) a recombinant vector, an expression cassette, a transgenic cell line or a recombinant bacterium containing a DNA molecule encoding the protein PIF 3;
the protein PIF3 is (1) or (2) as follows:
(1) a protein consisting of an amino acid sequence shown in a sequence 2 in a sequence table;
(2) and (b) the protein which is derived from the protein (1) and has the same function and is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown in the sequence 2 in the sequence table.
It is understood that one skilled in the art can substitute, delete and/or add one or several amino acids based on the disclosed amino acid sequences without affecting their activity to obtain mutant sequences of the proteins.
It is understood that, considering the degeneracy of codons and the preference of codons for different species, one skilled in the art can use codons suitable for the expression of a particular species as needed.
In the above application, the DNA molecule is any one of the following 1) to 3):
1) the coding region is a DNA molecule shown as a sequence 1 in a sequence table;
2) DNA molecules which hybridize under stringent conditions with the DNA sequences defined in 1) and which code for proteins having the same function;
3) a DNA molecule having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% homology with the DNA sequence defined in 1) and encoding a protein having the same function.
The above stringent conditions are hybridization at 65 ℃ in a 0.1 XSSPE containing 0.1% SDS or a 0.1 XSSC solution containing 0.1% SDS, and washing the membrane with the solution.
In the application, the insect resistance is the resistance to bemisia tabaci.
In the above application, the plant is a dicotyledonous plant or a monocotyledonous plant;
or the plant is a dicotyledon, and the dicotyledon is a cruciferae plant in particular; the cruciferous plant is specifically arabidopsis thaliana.
The application of any substance in 1) to 3) in breeding insect-resistant plants is also the protection scope of the invention;
or the application of any substance in the following 1) to 3) in serving or preparing pesticides is also the protection scope of the invention;
or the application of any substance of the following 1) to 3) in the disinsection is also the protection scope of the invention;
or, the application of any one of the following substances 1) to 3) in plant pest control and/or preparation of plant pest control products is also within the scope of the present invention;
1) protein PIF 3;
2) a DNA molecule encoding the protein PIF 3;
3) a recombinant vector, an expression cassette, a transgenic cell line or a recombinant bacterium containing a DNA molecule encoding the protein PIF 3.
In the application, the insect resistance is the resistance to bemisia tabaci;
or, the plant is a dicotyledonous plant or a monocotyledonous plant;
or the plant is a dicotyledon, and the dicotyledon is a cruciferae plant in particular; the cruciferous plant is specifically arabidopsis thaliana.
Another object of the present invention is to provide a method for breeding insect-resistant transgenic plants.
The method provided by the invention comprises the following steps: improving the expression quantity and/or activity of a DNA molecule of the protein PIF3 in a target plant to obtain a transgenic plant, wherein the insect resistance of the transgenic plant is higher than that of the target plant;
the protein PIF3 is (1) or (2) as follows:
(1) a protein consisting of an amino acid sequence shown in a sequence 2 in a sequence table;
(2) and (b) the protein which is derived from the protein (1) and has the same function and is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown in the sequence 2 in the sequence table.
In the above method, the expression level and/or activity of the DNA molecule encoding the protein PIF3 in the target plant is increased by introducing the DNA molecule encoding the protein PIF3 into the target plant.
In the method, the disease resistance is the resistance to bemisia tabaci;
in the above method, the plant is a dicotyledonous plant or a monocotyledonous plant;
or the plant is a dicotyledon, and the dicotyledon is a cruciferae plant in particular; the cruciferous plant is specifically arabidopsis thaliana.
Experiments prove that after the PIF3 gene is overexpressed, the gene is transferred into arabidopsis thaliana to obtain transgenic arabidopsis thaliana, the transgenic arabidopsis thaliana is expressed as a bemisia tabaci resistant phenotype, the gene or protein expressed by the gene is proved to have the effect of improving the insect resistance of plants, gene resources are provided for breeding new insect-resistant plant varieties, and the transgenic arabidopsis thaliana has good potential application value.
Drawings
FIG. 1 shows Western Blot detection results of plant proteins of strains of Arabidopsis thaliana overexpressed by PIF3 gene.
FIG. 2 shows the oviposition and number of pseudo pupae of Bemisia tabaci in wild type and PIF3 gene overexpression Arabidopsis thaliana.
FIG. 3 shows the amount of eggs laid and the number of false pupae of Bemisia tabaci on wild type and pifQ mutant Arabidopsis thaliana.
FIG. 4 is a double selection experiment of Bemisia tabaci on wild type and pifQ mutant Arabidopsis thaliana under different lighting conditions.
FIG. 5 shows the expression levels of the terpene synthase genes TPS10, TPS14 and TPS21 in wild type and pifQ mutant Arabidopsis thaliana under different illumination conditions.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The pENTR-3C cloning vector in the following examples is a commonly used cloning vector, and is commercially available; the arabidopsis variety is Columbia ecotype; the pifQ mutant is constructed in the laboratory, and the materials of pif1, pif3, pif4 and pif5 arabidopsis thaliana single-mutant used in the construction process are purchased from a SALK mutant library; agrobacterium EHA105 strain was purchased from Bomaide (Biomed) Bio Inc.
The pH7-WG2Y vector was a vector obtained by inserting YFP tag (SEQ ID NO: 5) into the PstI and BamHI cleavage sites of pH7-WG2 vector (purchased from Invitrogen).
The main reagents in the following examples are:
reagents used for molecular cloning: high fidelity DNA polymerase Phusion was purchased from Thermo Fisher Scientific; restriction enzymes were purchased from NEB; t4DNA ligase was purchased from NEB; 1kb DNA marker and 2000bp DNA marker were purchased from Bomader (Biomed); gelsafe was purchased from Hao, formerly; SYBR qPCR Mix was purchased from TOYOBO.
Reagents used for protein-related experiments: 40% Acrylamide from Sigma; cocktail protease inhibitors were purchased from Roche; coomassie Brilliant blue was purchased from Sigma; IPTG was purchased from Inolco; antibodies were purchased from Beijing Quanji Biotech; prestained protein molecular weight marker was purchased from EasyBIO corporation; developer is available from GE Healthcare; lucifersae was purchased from Sigma.
The plasmid miniprep kit and the DNA purification recovery kit are purchased from AxyGen company; plant DNA and RNA miniprep kits were purchased from Qiagen; the agarose gel DNA recovery kit is purchased from Beijing Quanzijin Biotech company; reverse transcription II one step DNA Removal and DNA Synthesis SuperMix kits were purchased from Kyoto Seiko Biotech, Inc.
Spectinomycin (Spe), rifampicin (Rif) and hygromycin (Hyg) were purchased from Inolco; the various other chemicals used in the examples were all imported or home-made analytical grade reagents.
The primers used in the examples were synthesized by the EnxElite Achiette corporation and subjected to related sequencing.
Example 1 cloning of PIF3 Gene and construction of vector for expressing PIF3 Gene
Cloning of PIF3 Gene
The nucleotide sequence of the cDNA of the PIF3 gene is shown as sequence 1, and the amino acid sequence of the encoded PIF3 protein is shown as sequence 2.
A CDS (sequence coding for aminoacids in protein) protein coding region DNA sequence 1 of the PIF3 gene was artificially synthesized, and an upstream primer F and a downstream primer R were designed based on the coding region sequence analysis.
An upstream primer F: 5'-CAAGGGTACCATGCCTCTGTTTGAGCTTTT-3' (sequence 3)
A downstream primer R: 5'-CAAGCTCGAGGACGATCCACAAAACTGATC-3' (sequence 4)
Taking DNA molecules shown in the sequence 1 as a template, and carrying out PCR amplification by using an upstream primer F and a downstream primer R to obtain a PCR amplification product of 1575 bp.
Secondly, construction of a vector for expressing PIF3
And (3) a vector 35S for expressing PIF3, wherein PIF3-YFP is a vector obtained by recombining a PIF3 gene shown in a sequence 1 into an expression vector pH7-WG2Y through LR reaction.
The construction method of the vector comprises the following steps of firstly connecting the obtained 1575bp PCR amplification product to a pENTR-3C cloning vector to obtain an intermediate vector; and performing LR reaction on the intermediate vector and the expression vector pH7-WG2Y to obtain a vector 35S for expressing PIF3, namely PIF3-YFP, and connecting the PIF3 gene to the expression vector pH7-WG2Y through sequencing verification.
Example 2 application of PIF3 gene in regulation and control of plant insect resistance
Construction of PIF3 transgenic plant expressing PIF3 gene
The PIF 3-expressing vector 35S prepared in example 1, PIF3-YF, was transformed into Agrobacterium EHA105 strain to obtain a recombinant Agrobacterium expressing PIF 3. And then transferring the recombinant strain into a wild type arabidopsis col-0 plant to obtain a PIF 3-transferred arabidopsis seedling.
The transformation method comprises the following specific steps: the recombinant Agrobacterium expressing PIF3 was inoculated in 5mL LB liquid medium containing antibiotics (rifampicin 50. mu.g/m L, spectinomycin 50. mu.g/m L) and cultured overnight at 28 ℃ at 220 rpm; transferring 5mL of LB liquid culture medium containing the recombinant agrobacterium expressing PIF3 into 250mL of liquid culture medium, and culturing for 16h at 28 ℃ and 220 rpm; collecting the bacterial liquid into a 250mL centrifugal barrel, centrifuging at 4000rpm for 15min, and collecting thalli; the cells were suspended in 400m L transformation medium (1/2MS, 5% sucrose, 40. mu.L Silwet); taking newly-flowering and well-growing wild type arabidopsis thaliana col-0 for transformation, cutting off green fruit pods by using scissors before transformation, then reversely buckling the arabidopsis thaliana in the resuspended agrobacterium for 10min, immersing a flower container of a plant in a bacterial liquid, adopting a pollen tube soaking method for transformation, flatly placing the arabidopsis thaliana in a pot, wrapping the arabidopsis thaliana by using a preservative film, and moving the arabidopsis thaliana to a dark place for 24 h; removing the cover the next day, lifting the plant and transferring to normal conditions for continuous culture; after about 3 weeks, seeds were harvested for selection of transformants.
Second, detecting
1. Hygromycin resistance selection
The selection resistance gene carried by the pH7-WG2Y vector is hygromycin, the hygromycin resistance is used for screening transformants, the hygromycin resistance is positive, T1 generation is used for transferring PIF3 Arabidopsis seedlings, a single plant is harvested, the hygromycin resistance test is carried out on T2 generation seeds, a strain with resistance 3/4 and no resistance in the rest 1/4 is selected, and the over-expression vector connected with the target gene in the strain is inserted in a single copy mode. And (3) removing the plants with hygromycin resistance from the strains, harvesting the single plants, screening the hygromycin resistance by using the obtained seeds of the T3 generations, if the plants of the T4 generations are not separated, indicating that the transgenic strains are homozygotes which can be used for seed reproduction and bemisia tabaci bioassay experiments, wherein the homozygotes are transformed from the seeds of PIF3 Arabidopsis thaliana of the T4 generation, and sowing the seeds of the PIF3 Arabidopsis thaliana of the T4 generation.
2. Detection by western blotting method
1) Extraction of plant Total protein
Taking 0.1g of T4 to replace a leaf of a PIF3 Arabidopsis plant, putting the leaf into a 1.5mL centrifuge tube, covering the centrifuge tube tightly, putting the centrifuge tube into liquid nitrogen for precooling, then grinding the centrifuge tube into powder by using a grinding rod, adding 500 mu L of 2 xSDS buffer, swirling the powder until the powder is mixed evenly, boiling the powder in boiling water for 5min to denature protein, centrifuging the powder at 10000rpm for 10min, and taking the supernatant for electrophoresis. Wild type Arabidopsis thaliana was used as a control.
2) Protein content in over-expressed strains was determined by Western Blot
The same amount of wild type and plant protein of T4 generation transformed PIF3 Arabidopsis plant (35S: PIF3-YFP) are respectively taken, electrophoresis is carried out by using a voltage stabilizing mode, the electrophoresis is firstly carried out for about 20min by using 80V voltage until protein bands run out of separation gel, and the electrophoresis is carried out for about 1h by transforming 100V voltage. And finally, detecting the protein content in the over-expression strain by using an Anti-YFP antibody. To be provided with
Anti-Actin serves as an internal reference.
The results of the WesternBlot test are shown in FIG. 1, and it can be seen that the target protein is strongly expressed by the T4 generation PIF3 Arabidopsis plant (35S: PIF 3-YFP).
The empty vector pH7-WG2Y vector is transferred into wild arabidopsis thaliana by the same method to obtain T4 transfer empty vector arabidopsis thaliana.
Methods for overexpression of PIF3 gene in other plants can be performed with reference to this example.
Third, the insect resistance detection of the transgenic PIF3 Arabidopsis thaliana
Bemisia tabaci MeAM1(mtCOI, GenBank accession MF579701) was used as a Bemisia tabaci for experiments and was captured on tomato plants in the sunward region of Beijing. The bemisia tabaci is bred in an insect breeding cage, and the cotton is used as a host plant for growth and propagation. The indoor temperature is 25 ℃, the relative humidity is 65%, and the illumination period is 12h of illumination/12 h of darkness.
Firstly, wild type arabidopsis seedlings, T4 empty vector-transferred arabidopsis seedlings and T4 generation-transferred PIF3 arabidopsis plants (PIF3-OE) grow on a culture dish for 21 days, then the seedlings are transferred to soil to grow for 14 days, and a tobacco whitefly growth test experiment is carried out by adopting a leaf cage method: in the bemisia tabaci oviposition experiment, 3 female insects and 3 male insects are placed in a leaf cage (diameter 45 mm; height 30mm), and the number of eggs is counted after 10 days. In the experiment of the development of the bemisia tabaci pseudo pupae, 16 female insects are placed in a leaf cage, after the egg laying amount of the female insects reaches 2 days, all the adult bemisia tabaci are removed, and the eggs are left to continue to develop in the leaf cage; and counting the number of fake pupae after 20 days. 8 strains of each strain, the experiment was repeated 3 times, and the results were averaged.
As shown in FIG. 2, the oviposition amount (FIG. 2A) and the number of pseudo pupae (FIG. 2B) of Bemisia tabaci on T4 generation transgenic PIF3 Arabidopsis plants (PIF3-OE) were significantly reduced compared to wild type Arabidopsis thaliana. This shows that the insect resistance of the T4 generation PIF3 Arabidopsis plant (PIF3-OE) is obviously improved, and the PIF3 overexpression improves the insect resistance of the plant.
The results of the wild type arabidopsis seedlings and the T4 empty vector arabidopsis seedlings have no significant difference.
Example 3 PIF3 Gene regulating plant insect resistance
Construction of pifQ mutants (pif1, pif3, pif4, pif5)
The purchased pif1, pif3, pif4 and pif5 mutants arabidopsis thaliana (only the pif1, pif3, pif4 or pif5 genes are inactivated compared with the wild type) are sown, arabidopsis thaliana plants which just blossom and bolt and have good growth conditions are selected, pif1 and pif5 are hybridized, and pif3 and pif4 are hybridized. Carefully selecting the buds which do not bloom in the female parent plant, and completely removing stamens, petals and sepals by using a pointed-end forceps to leave the stigma. And (4) repeatedly smearing the stamens of the flowers of the male parent just blossomed on the stigma of the female parent, and filling the pores above the stigma with anthers. After 2-3 days, the stigma is observed to obviously extend and become thick, which represents successful hybridization, and if the change is not too large or the stigma slowly withers, the hybridization fails, and the stigma needs to be redone in time. After the seeds are mature, the hybrid seeds are carefully harvested. F1 plants of pif1/pif5 are continuously crossed with F1 plants of pif3/pif4, F2 generation seeds of pif1/pif3/pif4/pif5 are paved on an MS flat plate, plant markers with completely opened cotyledons are selected after 3 days of growth in the dark, the plants are moved into soil for continuous growth after light adaptation treatment, after the plants are mature, single plant genome DNA is taken, and four loci of fixed pif1/pif3/pif4/pif5 are homozygous, so that the pifQ four-mutant plants are obtained.
II, detection of insect resistance of pifQ mutant
Wild type Arabidopsis thaliana and pifQ mutant plants were tested for insect resistance according to the third example 2.
As a result, as shown in FIG. 3, the number of eggs laid by Bemisia tabaci (FIG. 3A) and the number of pseudo pupae (FIG. 3B) were significantly increased in the pifQ mutant plants as compared with that of wild type Arabidopsis thaliana. This indicates that the insect resistance of the pifQ mutant plants is reduced, and further verifies the insect resistance of the PIF3 overexpression plants.
Tri, PifQ mutant bemisia tabaci double selection experiment
Selecting wild arabidopsis thaliana and pifQ mutant arabidopsis thaliana with consistent plant size and leaf number, and selecting 5 illumination conditions: dark, natural light, red light (770-622 nm), far-red light (735-730 nm) and blue light (492-455 nm), treating for 2 hours under different illumination conditions, and placing into an insect cage (30 x 30 cm); freezing about 200 Bemisia tabaci on ice for 1min, then releasing between two plants, carrying out the selection process under the same illumination condition, and counting and recording the number of Bemisia tabaci on each plant after the Bemisia tabaci is selected for 25 min; 6 biological replicates were selected for each experiment.
The results are shown in fig. 4, and it can be seen that bemisia tabaci is more prone to select pifQ mutant plants than wild type arabidopsis under natural light and red light conditions, which indicates that the insect resistance of the pifQ mutant plants is reduced, and further verifies the insect resistance of the plants over-expressed by PIF 3.
Fourthly, the gene expression level of the PifQ mutant terpene synthase genes TPS10, TPS14 and TPS21
Treating the wild arabidopsis thaliana and the pifQ mutant arabidopsis thaliana under the illumination condition of the third step, extracting sample RNA by using an RNA extraction kit after sampling, inverting by using a reverse transcription kit to obtain cDNA, and detecting the gene expression amounts of the repellent terpene compounds TPS10, TPS14 and TPS21 of the wild arabidopsis thaliana and the pifQ mutant arabidopsis thaliana under different illumination.
As shown in fig. 5, under natural light and red light conditions, compared with wild arabidopsis thaliana, the gene expression levels of the repellent terpene compounds TPS10 (fig. 5A), TPS14 (fig. 5B) and TPS21 (fig. 5C) in the pifQ mutant arabidopsis thaliana were significantly reduced, and were more attractive to bemisia tabaci.
Therefore, after the Arabidopsis PIF3 gene is over-expressed, the resistance of the plant to bemisia tabaci can be remarkably improved, and after the PIF3 gene and homologous genes PIF1, PIF4 and PIF5 thereof are knocked out, the resistance of the plant to bemisia tabaci is remarkably reduced.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Sequence listing
<110> institute of microbiology of Chinese academy of sciences university of Chinese academy of sciences
<120> application of Arabidopsis thaliana transcription factor gene PIF3 in insect stress resistance of plants
<160> 5
<170> PatentIn version 3.5
<210> 1
<211> 1575
<212> DNA
<213> Artificial Sequence
<400> 1
atgcctctgt ttgagctttt caggctcacc aaagctaagc ttgaatctgc tcaagacagg 60
aacccttctc cacctgtaga tgaagttgtg gagctggtgt gggaaaatgg tcagatatca 120
actcaaagtc agtcaagtag atcgaggaac attcctccac cacaagcaaa ctcttctaga 180
gctagagaga ttggaaatgg ctcaaagacg actatggtgg acgagatccc tatgtcagtg 240
ccatcactaa tgacgggttt gagtcaagac gatgactttg ttccatggtt gaatcatcat 300
ccctcccttg atggatattg ctctgatttc ttgcgtgatg tgtcgtctcc tgttactgtc 360
aacgagcaag agagtgatat ggcggtaaac caaactgctt tcccgttgtt tcagagaaga 420
aaggatggca atgaatcagc tcctgctgct tcttcgtcgc agtataacgg tttccaatcg 480
cattctctgt atggaagtga tagagctaga gatcttccta gccaacaaac caatccggat 540
cggtttactc agacgcagga accactaatt actagtaaca agcctagttt ggtcaacttt 600
tcacatttct tacgccctgc aacttttgcg aagactacta ataataacct tcatgacact 660
aaagaaaaga gtcctcaaag cccgccaaat gtgtttcaga ccagagttct tggagctaaa 720
gactctgaag ataaggttct taacgagtct gttgcttctg ctacgcctaa agataaccaa 780
aaggcttgcc taatatcaga ggactcatgt agaaaagacc aagagagtga aaaagcagtt 840
gtatgttctt ctgttggctc gggtaatagt ctcgatggcc catccgaaag tccttcactt 900
tctttaaaga gaaagcattc gaatattcaa gacattgact gtcatagtga agatgtggaa 960
gaagaatcag gagatggaag aaaggaagca ggtccatctc gaacgggttt gggttcaaag 1020
agaagccgct ctgcagaagt gcataatctg tctgaaagga gacggcgtga taggatcaac 1080
gagaagatgc gtgccctgca agaactcatt ccaaactgta acaaggtgga caaagcttcg 1140
atgctagatg aagccatcga gtatctcaag tcactccaac ttcaagtgca gatcatgtca 1200
atggcgtctg gttactatct gccaccggcg gttatgttcc caccgggtat ggggcattac 1260
ccggcagcag ctgctgcaat ggcaatgggt atgggaatgc cttatgcaat gggcttgcct 1320
gatttgagcc gtggtggttc atcggttaac cacggaccac agttccaagt ctcggggatg 1380
caacaacaac cagtggcgat gggtattcca cgtgtctctg gtggtggtat ctttgccggt 1440
tcttcgacga ttggcaatgg ctcgactaga gatttatctg gttctaaaga tcaaacaacg 1500
acgaataaca acagtaactt gaaaccaata aagagaaaac aggggtcttc tgatcagttt 1560
tgtggatcgt cgtga 1575
<210> 2
<211> 524
<212> PRT
<213> Artificial Sequence
<400> 2
Met Pro Leu Phe Glu Leu Phe Arg Leu Thr Lys Ala Lys Leu Glu Ser
1 5 10 15
Ala Gln Asp Arg Asn Pro Ser Pro Pro Val Asp Glu Val Val Glu Leu
20 25 30
Val Trp Glu Asn Gly Gln Ile Ser Thr Gln Ser Gln Ser Ser Arg Ser
35 40 45
Arg Asn Ile Pro Pro Pro Gln Ala Asn Ser Ser Arg Ala Arg Glu Ile
50 55 60
Gly Asn Gly Ser Lys Thr Thr Met Val Asp Glu Ile Pro Met Ser Val
65 70 75 80
Pro Ser Leu Met Thr Gly Leu Ser Gln Asp Asp Asp Phe Val Pro Trp
85 90 95
Leu Asn His His Pro Ser Leu Asp Gly Tyr Cys Ser Asp Phe Leu Arg
100 105 110
Asp Val Ser Ser Pro Val Thr Val Asn Glu Gln Glu Ser Asp Met Ala
115 120 125
Val Asn Gln Thr Ala Phe Pro Leu Phe Gln Arg Arg Lys Asp Gly Asn
130 135 140
Glu Ser Ala Pro Ala Ala Ser Ser Ser Gln Tyr Asn Gly Phe Gln Ser
145 150 155 160
His Ser Leu Tyr Gly Ser Asp Arg Ala Arg Asp Leu Pro Ser Gln Gln
165 170 175
Thr Asn Pro Asp Arg Phe Thr Gln Thr Gln Glu Pro Leu Ile Thr Ser
180 185 190
Asn Lys Pro Ser Leu Val Asn Phe Ser His Phe Leu Arg Pro Ala Thr
195 200 205
Phe Ala Lys Thr Thr Asn Asn Asn Leu His Asp Thr Lys Glu Lys Ser
210 215 220
Pro Gln Ser Pro Pro Asn Val Phe Gln Thr Arg Val Leu Gly Ala Lys
225 230 235 240
Asp Ser Glu Asp Lys Val Leu Asn Glu Ser Val Ala Ser Ala Thr Pro
245 250 255
Lys Asp Asn Gln Lys Ala Cys Leu Ile Ser Glu Asp Ser Cys Arg Lys
260 265 270
Asp Gln Glu Ser Glu Lys Ala Val Val Cys Ser Ser Val Gly Ser Gly
275 280 285
Asn Ser Leu Asp Gly Pro Ser Glu Ser Pro Ser Leu Ser Leu Lys Arg
290 295 300
Lys His Ser Asn Ile Gln Asp Ile Asp Cys His Ser Glu Asp Val Glu
305 310 315 320
Glu Glu Ser Gly Asp Gly Arg Lys Glu Ala Gly Pro Ser Arg Thr Gly
325 330 335
Leu Gly Ser Lys Arg Ser Arg Ser Ala Glu Val His Asn Leu Ser Glu
340 345 350
Arg Arg Arg Arg Asp Arg Ile Asn Glu Lys Met Arg Ala Leu Gln Glu
355 360 365
Leu Ile Pro Asn Cys Asn Lys Val Asp Lys Ala Ser Met Leu Asp Glu
370 375 380
Ala Ile Glu Tyr Leu Lys Ser Leu Gln Leu Gln Val Gln Ile Met Ser
385 390 395 400
Met Ala Ser Gly Tyr Tyr Leu Pro Pro Ala Val Met Phe Pro Pro Gly
405 410 415
Met Gly His Tyr Pro Ala Ala Ala Ala Ala Met Ala Met Gly Met Gly
420 425 430
Met Pro Tyr Ala Met Gly Leu Pro Asp Leu Ser Arg Gly Gly Ser Ser
435 440 445
Val Asn His Gly Pro Gln Phe Gln Val Ser Gly Met Gln Gln Gln Pro
450 455 460
Val Ala Met Gly Ile Pro Arg Val Ser Gly Gly Gly Ile Phe Ala Gly
465 470 475 480
Ser Ser Thr Ile Gly Asn Gly Ser Thr Arg Asp Leu Ser Gly Ser Lys
485 490 495
Asp Gln Thr Thr Thr Asn Asn Asn Ser Asn Leu Lys Pro Ile Lys Arg
500 505 510
Lys Gln Gly Ser Ser Asp Gln Phe Cys Gly Ser Ser
515 520
<210> 3
<211> 30
<212> DNA
<213> Artificial Sequence
<400> 3
caagggtacc atgcctctgt ttgagctttt 30
<210> 4
<211> 30
<212> DNA
<213> Artificial Sequence
<400> 4
caagctcgag gacgatccac aaaactgatc 30
<210> 5
<211> 717
<212> DNA
<213> Artificial Sequence
<400>5
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60
ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120
ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180
ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag 240
cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360
gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420
aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480
ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540
gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600
tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660
ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag 717
Claims (11)
1. The application of any one of the following substances 1) to 3) in improving the resistance of plants to bemisia tabaci:
1) protein PIF 3;
2) a DNA molecule encoding the protein PIF 3;
3) a recombinant vector, an expression cassette, a transgenic cell line or a recombinant bacterium containing a DNA molecule encoding the protein PIF 3;
the protein PIF3 is composed of an amino acid sequence shown in a sequence 2 in a sequence table.
2. Use according to claim 1, characterized in that: the DNA molecule is a DNA molecule with a coding region shown as a sequence 1 in a sequence table.
3. Use according to any one of claims 1-2, characterized in that: the plant is a dicotyledonous plant or a monocotyledonous plant.
4. Use according to claim 3, characterized in that: the dicotyledonous plant is a crucifer; the cruciferous plant is specifically arabidopsis thaliana.
5. The application of any one of the following substances 1) to 3) in cultivating the bemisia tabaci resistant plants;
or, the application of any one of the following substances 1) to 3) in serving as or preparing pesticide for killing bemisia tabaci;
or, any one of the following substances 1) to 3) is applied to killing the bemisia tabaci;
or, any substance in the following 1) to 3) is applied to the control of the bemisia tabaci pests of plants and/or the preparation of products for controlling the bemisia tabaci pests of plants;
1) protein PIF 3;
2) a DNA molecule encoding the protein PIF 3;
3) a recombinant vector, an expression cassette, a transgenic cell line or a recombinant bacterium containing a DNA molecule encoding the protein PIF 3;
the protein PIF3 is composed of an amino acid sequence shown in a sequence 2 in a sequence table.
6. Use according to claim 5, characterized in that: the plant is a dicotyledonous plant or a monocotyledonous plant.
7. Use according to claim 6, characterized in that:
the plant is a dicotyledon, and the dicotyledon is a cruciferae plant; the cruciferous plant is specifically arabidopsis thaliana.
8. A method for cultivating transgenic plants with high resistance to Bemisia tabaci comprises the following steps: increasing the expression level of a DNA molecule of the protein PIF3 coded in a target plant to obtain a transgenic plant, wherein the resistance of the transgenic plant to bemisia tabaci is higher than that of the target plant;
the protein PIF3 is a protein consisting of an amino acid sequence shown in a sequence 2 in a sequence table.
9. The method of claim 8, wherein:
the expression level of the DNA molecule coding for the protein PIF3 in the target plant is improved by introducing the DNA molecule coding for the protein PIF3 into the target plant.
10. The method according to any one of claims 8-9, wherein: the plant is a dicotyledonous plant or a monocotyledonous plant.
11. The method of claim 10, wherein:
the plant is a dicotyledon, and the dicotyledon is a cruciferae plant; the cruciferous plant is specifically arabidopsis thaliana.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810378111.4A CN110396125B (en) | 2018-04-25 | 2018-04-25 | Application of arabidopsis transcription factor gene PIF3 in insect stress resistance of plants |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810378111.4A CN110396125B (en) | 2018-04-25 | 2018-04-25 | Application of arabidopsis transcription factor gene PIF3 in insect stress resistance of plants |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110396125A CN110396125A (en) | 2019-11-01 |
CN110396125B true CN110396125B (en) | 2021-08-13 |
Family
ID=68322010
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810378111.4A Expired - Fee Related CN110396125B (en) | 2018-04-25 | 2018-04-25 | Application of arabidopsis transcription factor gene PIF3 in insect stress resistance of plants |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110396125B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111909252B (en) * | 2020-09-25 | 2021-11-23 | 中国农业科学院特产研究所 | Ginseng PgbHLH149 transcription factor and application thereof |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040216190A1 (en) * | 2003-04-28 | 2004-10-28 | Kovalic David K. | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
US20090144849A1 (en) * | 2002-02-11 | 2009-06-04 | Lutfiyya Linda L | Nucleic acid molecules and other molecules associated with transcription in plants |
CN102686604A (en) * | 2009-08-19 | 2012-09-19 | 巴斯夫植物科学有限公司 | Plants having enhanced yield-related traits and a method for making the same |
CN107586324A (en) * | 2016-07-06 | 2018-01-16 | 中国农业科学院作物科学研究所 | TabZIP15 albumen and its encoding gene and application |
-
2018
- 2018-04-25 CN CN201810378111.4A patent/CN110396125B/en not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090144849A1 (en) * | 2002-02-11 | 2009-06-04 | Lutfiyya Linda L | Nucleic acid molecules and other molecules associated with transcription in plants |
US20040216190A1 (en) * | 2003-04-28 | 2004-10-28 | Kovalic David K. | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
CN102686604A (en) * | 2009-08-19 | 2012-09-19 | 巴斯夫植物科学有限公司 | Plants having enhanced yield-related traits and a method for making the same |
CN107586324A (en) * | 2016-07-06 | 2018-01-16 | 中国农业科学院作物科学研究所 | TabZIP15 albumen and its encoding gene and application |
Non-Patent Citations (4)
Title |
---|
How plants handle multiple stresses: hormonal interactions underlying responses to abiotic stress and insect herbivory;Duy Nguyen;《Plant Mol Biol》;20161231;第91卷;第727–740页 * |
PIF3 Is Involved in the Primary Root Growth Inhibition of Arabidopsis Induced by Nitric Oxide in the Light;Sulan Bai;《Molecular Plant》;20140430;第7卷(第4期);第616–625页 * |
Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly);Jianying Li;《Plant Biotechnology Journal》;20161231;第14卷;第1956–1975页 * |
ZmPIFs基因的克隆、表达及AtPIFs基因的抗旱功能研究;任小芸;《中国优秀硕士学位论文全文数据库 基础科学辑》;20180115(第 01 期);A006-73 * |
Also Published As
Publication number | Publication date |
---|---|
CN110396125A (en) | 2019-11-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2005336142B2 (en) | A transgenic plant having enhanced drought tolerance | |
CN109576282B (en) | Chinese rose transcription factor RhMYB4 and application thereof in flower organ development regulation | |
CN110317250B (en) | Application of MYB6 gene and encoding protein thereof in regulation and control of verticillium wilt resistance of plants | |
CN104093842A (en) | Improving plant drought tolerance, nitrogen use efficiency and yield | |
WO2018010513A1 (en) | Cotton ghtcp4 gene and application thereof in modifying length of cotton fiber | |
CN107299103A (en) | Thick boisiana IpASR genes and its encoding proteins and application | |
CN110592137B (en) | Application of arabidopsis AT5G10290 gene and mutant thereof in improving drought tolerance of plants | |
CN117660478A (en) | Gene for improving resistance of potatoes to late blight and application thereof | |
JPWO2008020645A1 (en) | Early transformed plant | |
CN113621643A (en) | Application of GhTULP34 in regulation and control of plant resistance to abiotic adversity stress and regulation and control method | |
CN112626084A (en) | Strawberry MYB transcription factor FvMYB24 gene, expression protein and application | |
CN110396125B (en) | Application of arabidopsis transcription factor gene PIF3 in insect stress resistance of plants | |
CN115160422B (en) | Salt-tolerant drought-resistant related protein IbMYB44 of sweet potato, and coding gene and application thereof | |
CN117264964A (en) | Application of wheat TaGSKB protein and encoding gene thereof in regulation and control of plant stress tolerance | |
CN111826390B (en) | Application of protein WRKY78 in regulation of plant biotic stress resistance | |
CN112322651A (en) | Application of tomato autophagy gene in improving plant root-knot nematode resistance | |
CN108866074B (en) | Application of herbicide-resistant gene PAR3(G311E) | |
US20220042030A1 (en) | A method to improve the agronomic characteristics of plants | |
CN116063431B (en) | Plant insect-resistant protein and application thereof | |
CN115044592B (en) | Gene ZmADT2 for regulating and controlling maize plant type and resistance to tumor smut, and encoding protein and application thereof | |
WO2011110099A1 (en) | Enhanced plant-mediated rna interference in insects by utilizing cysteine protease | |
US20230225331A1 (en) | Plant Endophytic Bacteria And Methods To Control Plant Pathogens And Pests | |
CN109678940B (en) | Protein BhDnaJ6, and coding gene and application thereof | |
CN105177030B (en) | The FvSnRK2.1 genes of Fraxinus velutina salt tolerant and its application | |
KR101360542B1 (en) | RICE OsSERK1 GENE AND TRANSGENIC RICE TRANSFORMED WITH THE SAME |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210813 |