CN110393726A - Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation and preparation method thereof of light moderate acne - Google Patents
Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation and preparation method thereof of light moderate acne Download PDFInfo
- Publication number
- CN110393726A CN110393726A CN201910816379.6A CN201910816379A CN110393726A CN 110393726 A CN110393726 A CN 110393726A CN 201910816379 A CN201910816379 A CN 201910816379A CN 110393726 A CN110393726 A CN 110393726A
- Authority
- CN
- China
- Prior art keywords
- stem cell
- preparation
- parts
- dried powder
- treats
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/238—Saposhnikovia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
Abstract
Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation and preparation method thereof of light moderate acne, it is related to stem cell medicine and application in TCM field.It will solve the problems, such as the preparation of existing treatment acne, and that there are effects is poor.Preparation: Stem Cell Activity factor freeze-dried powder, Flos Lonicerae extractive solution and mixing herb powder are made；Mixing herb powder is made of radix scutellariae, the root of Dahurain angelica, rhizoma typhonii, radix saposhnikoviae, Radix Salviae Miltiorrhizae and mung bean.Method: taking umbilical cord and adipose-derived mescenchymal stem cell, mixed culture, receives P3-P6 for cell supernatant；Mixing, filtering add mannitol, prepare freeze-dried powder after packing, pre-cooling；Prepare Flos Lonicerae extractive solution；Preparation mixing herb powder.The light moderate acne effect of present invention treatment is preferable, is not likely to produce dependence, can eliminate acne print or inhibit the generation of acne and whelk, reduce and inhibit the generation of fine wrinkles.Preparation method is simple to operation, low in cost, has no toxic side effect.The present invention prepares gained preparation for treating light moderate acne.
Description
Technical field
The present invention relates to stem cell medicines and application in TCM field, and in particular in Stem Cell Activity factor freeze-dried powder joint
Medicine treats the preparation and preparation method thereof of light moderate acne.
Background technique
Mescenchymal stem cell is the early stage cell of mesoderm development.Now act as the mescenchymal stem cell of scientific research mostly from
Adult bone marrow obtains, also known as marrow stromal cell.Mescenchymal stem cell has the fundamental characteristics of stem cell, expands such as self and more
To differentiation property.Under the different phase and certain environmental conditions of development, mescenchymal stem cell can be divided into various kinds of cell.MSCs
The bioactie agent of paracrine mainly has vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), angiogenin
(Ang), brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), transforming growth factor-β (TGF-β), leucocyte
Interleukin -10 (IL-10), interleukin 1 (IL-1), tumor necrosis factor (TNF) and chemotactic factor (CF) etc..Stem cell is being organized
It repairs and has broad application prospects with regeneration aspect, be widely used in translational medicine field, but stem cell plays at present
Effect, mechanism is not also fully aware of.Recent studies indicate that tissue repair and regeneration function that stem cell is played are very
It is to be realized by its paracrine action, rather than it is in the proliferation and differentiation of damage location in big degree.
Cicatrization and do not recover for a long time be soft tissue injury treatment two hang-ups.Discovered in recent years umbilical cord mesenchyma is dry thin
The indexing of β-chain of rings catenin and vigor can be improved in the wnt4 that born of the same parents source excretion body (HucMSC-Exo) includes, to promote skin
The proliferation and migration and angiogenesis of skin cell.Research finds that fat mesenchymal stem cell source excretion body (ASCs-Exo) can
With dosage-dependent manner stimulation it is fibroblastic proliferation, migration and collagen synthesis, and promote cyclin 1,
The expression of N- calcium mucin, Type I collagen, III Collagen Type VI and proliferating cell nuclear antigen promotes to promote the healing of skin wound
The formation of blood vessel endothelium tip cell adjusts inner cell angiogenesis function.
In recent years to neonatal Cord blood, placenta, umbilical cord research is all discarded after fetal birth gradually deeply
Object, amoral dispute of ethic are the source of ideal mescenchymal stem cell.The growth factor of stem cell secretion is active peptides object
Matter strongly facilitates the proliferation of human skin cell, growth, safely, effectively.Major function is quick reparation damaged skin, and
Reparation, the nursing of improvement property skin；Eye wrinkle-removing, firm skin reduce the average age of skin histology, moisturize the skin, and increase
Strong skin elasticity, whitening spot-removing reduce the content of melanin and coloured corpuscle in Skin Cell, and it is de- to accelerate melanocyte metabolism
It falls, sun-proof and after-sun recovery, mitigates injury of the ultraviolet radiation to skin, reduce the blackspot growth of skin after shining, anti-acne is gone
Scar shortens the wound healing time and reduces scar being formed, has good effect to preventing and nursing acne.
The non-inflammation skin of acne damage of acne shows as open and closed comedones.The typical case of closed comedones (also known as hoary hair)
Skin lesion is the colour of skin papule of about 1 mm in size, without obvious hair follicle opening.Open comedones (also known as blackhead) show as dome-shaped mound
Rash is with the hair follicle opening significantly expanded.Local topical drug can with tretinoin, benzoyl peroxide, antibiotics, azelaic acid,
Sulphur lotion etc., and treatment acne most efficient method at present, but even if be also easy to go out to leave in skin after curing acne
Scar, subcutaneous melanin deposition, skin affected part black over time, and acne print is difficult to eliminate, is not easy to stay scar so should select
Treatment method reduces spot, acne print, the generation of scar to treating in time at epidermis or ulceration.
The growth factor of stem cell secretion is activity polypeptid substance, can strongly facilitate proliferation, the life of human skin cell
It is long.Safely, effectively.Major function is quickly reparation damaged skin, and reparation, the nursing of improvement property skin；Eye wrinkle-removing, tightly
Skin is caused, the average age of skin histology is reduced, is moisturized the skin, skin elasticity is enhanced；Whitening spot-removing reduces in Skin Cell
The content of melanin and coloured corpuscle accelerates melanocyte metabolism and falls off；Sun-proof and after-sun recovery mitigate ultraviolet radiation to skin
The injury of skin reduces the blackspot growth of skin after shining；Anti-acne removes scar, shortens the wound healing time and reduces scar shape
At having good effect to preventing and nursing acne.But a variety of stem cell freeze-dried powders of commercial type at present, but be lyophilized
In powder because subcategory it is more single, the content of the factor is indefinite, and activity is indefinite, if sterile indefinite, and using effect
Also undesirable.
Summary of the invention
The invention aims to solve the problems, such as the preparation of existing treatment acne, that there are effects is poor, and provides stem cell work
Sex factor freeze-dried powder Combined with Chinese Herbal treats the preparation and preparation method thereof of light moderate acne.
Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation of light moderate acne, it is in parts by weight by 1~2 part
Stem Cell Activity factor freeze-dried powder, 8~12 parts of honeysuckle liquid and 8~20 parts of mixing herb powder be made；It is wherein described
Herb powder is mixed in parts by weight by 10 parts of radix scutellariae, 10 parts of the root of Dahurain angelica, 15 parts of rhizoma typhonii, 25 parts of radix saposhnikoviae, 8 parts of pellet
Ginseng and 5 parts of mung bean are made.
Above-mentioned Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation method of light moderate acne preparation, it is by following
Step carries out:
One, secondary culture is carried out to the mescenchymal stem cell in umbilical cord source, take P3 for mescenchymal stem cell (0.5~1) ×
106It is a；
Two, secondary culture is carried out to adipose-derived mescenchymal stem cell, take P3 for mescenchymal stem cell (0.5~1) ×
106It is a；
Three, the P3 in step 1 and step 2 is mixed for mescenchymal stem cell, is then inoculated in 20ml free serum culture
In base, then it is placed in 37 DEG C, CO2Cultivate 3~5 days in the incubator that volumetric concentration is 5%, then collection P3 is for cell supernatant,
It is sub-packed in sterile centrifugation tube, is saved at 4 DEG C；
Four, continue to cultivate according to the operating process of step 1 to step 3, collect P4, P5 and P6 respectively for cell conditioned medium
Liquid is sub-packed in sterile centrifugation tube, is saved at 4 DEG C；
Five, P3, P4, P5 and P6 are mixed for cell supernatant, by 0.22 μm of filter filtration sterilization, is protected in 4 DEG C of refrigerators
It deposits, takes mixing supernatant 10ml, 2~6g mannitol is added, is then sub-packed in cillin bottle, every bottle of 2~4ml, is pre-chilled 2 at -80 DEG C
Then~4h carries out the production of freeze-dried powder and is stored at 4 DEG C to save；
Six, 20 parts of honeysuckle is added in 100 parts of 100 DEG C of water in parts by weight, intense fire boils 30min, then literary
Fire boils 20min, then by 0.22 μm of filter filtering, is placed in the sterile liquid storage bottle of 100ml, as honeysuckle liquid, protects at 4 DEG C
It deposits；
Seven, 10 parts of radix scutellariae, 10 parts of the root of Dahurain angelica, 15 parts of rhizoma typhonii, 25 parts of radix saposhnikoviae, 8 parts of pellet are taken in parts by weight
Ginseng and 5 parts of mung bean, grind into powder after mixing are placed in sterile liquid storage bottle, are as mixed by the strainer filtering of 100 mesh
Herb powder saves in a cool and dry place, i.e. completion Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne system
The preparation of agent.
The invention has the advantages that
1, Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne preparation in the present invention, and it is living to choose stem cell
Sex factor Combined with Chinese Herbal radix scutellariae, the root of Dahurain angelica, rhizoma typhonii, honeysuckle, radix saposhnikoviae, Radix Salviae Miltiorrhizae, mung bean are treated, and therapeutic effect is preferable, right
Situations such as skin zero damage is not likely to produce dependence, will not generate hormone face can eliminate acne print or inhibit acne and whelk
Generation, while the Stem Cell Activity factor is improving acne and while whelk, can reduce and inhibit skin fine wrinkles
Generation, have both moisture-keeping efficacy, the two is used in combination, and can play preferable therapeutic effect.
2, the present invention in Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treat light moderate acne preparation preparation method letter
Single easy to operate, Chinese medicine is low in cost, has no toxic side effect, and effect is good.
3, Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation method of light moderate acne preparation in the present invention, adopts
With umbilical cord mesenchymal stem cells and fat mesenchymal stem cell culture P3-P6 for the supernatant of cell, supernatant is interior to contain more type
Cell factor repair aspect and play a significant role, and cell factor liquid wants cryo-conservation in soft tissue regeneration, keep thin
The activity and function of intracellular cytokine.Cell factor liquid facilitates preservation after freeze-dried powder is made, and can protect the activity and function of cell factor
Can, it plays one's part to the full, it is also more convenient when in use.
4, in existing commercially available stem cell freeze-dried powder because subcategory it is more single, the content of the factor is indefinite, activity it is indefinite,
It is whether sterile indefinite, and using effect is also not highly desirable, the stem cell factor freeze-dried powder prepared in the present invention, because using
The mescenchymal stem cell co-incubation of umbilical cord and fatty two kinds of sources, and cultivated for 3 generations, can also higher generation culture, will be each
Supernatant after culture mixes after collecting, the stem cell in two kinds of sources can sufficiently secrete the cell of separate sources because
Son, because subcategory is more, and more generation cultures, content is richer, the effect of active factors can be given full play to when in use,
And preparation process is sterile, and is detected by multilayer, use process safety, in the case of especially having ulceration on the face, Neng Gouan
It entirely uses, not will lead to the generation of infection.
5, the Stem Cell Activity factor freeze-dried powder prepared in the present invention can be repaired quickly, nurse damaged skin；Improve eye
Portion's wrinkle, firm skin reduce the average age of skin histology, moisturize the skin, and enhance skin elasticity；Whitening spot-removing reduces
The content of melanin and coloured corpuscle in Skin Cell accelerates melanocyte metabolism and falls off；Sun-proof and after-sun recovery mitigate ultraviolet
Injury of the beta radiation to skin reduces the blackspot growth of skin after shining；Anti-acne removes scar, shorten the wound healing time and
It reduces scar to be formed, has good effect to preventing and nursing acne.
6, Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne preparation in the present invention, according to whelk and
The severity that acne occurs is configured to Chinese medicine cream and embrocated, and can be effectively improved the generation and acne print of whelk and acne
Improve, and skin does not have dependence, allergic reaction will not be generated, and while improving whelk and acne occurs, carefully
Intracellular cytokine liquid can improve skin elasticity, reduce the generation of fine wrinkles.
The present invention prepares gained Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal and treats light moderate acne preparation for treating
Light moderate acne.
Detailed description of the invention
Fig. 1 is that VISIA detects the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne in embodiment
Agent, the figure before treating light moderate face acnes acne print treatment；
Fig. 2 is that VISIA detects the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne in embodiment
Agent, the figure after treating light moderate face acnes acne print treatment；
Fig. 3 is that VISIA detects the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne in embodiment
Agent, the figure of eye pouch wrinkle before facial treatments；
Fig. 4 is that VISIA detects the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne in embodiment
Agent, the figure of eye pouch wrinkle after facial treatments.
Specific embodiment
The technical solution of the present invention is not limited to the following list, further includes between each specific embodiment
Any combination.
Specific embodiment 1: present embodiment Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne
Preparation, it is in parts by weight by 1~2 part of Stem Cell Activity factor freeze-dried powder, 8~12 parts of honeysuckle liquid and 8~20 parts
Mixing herb powder is made；Wherein the mixing herb powder is in parts by weight by 10 parts of radix scutellariae, 10 parts of the root of Dahurain angelica, 15 parts
Rhizoma typhonii, 25 parts of radix saposhnikoviae, 8 parts of Radix Salviae Miltiorrhizae and 5 parts of mung bean are made.
Honeysuckle is clearing heat and detoxicating in present embodiment, anti-infective；Radix scutellariae heat-clearing and damp-drying drug, removing pattogenic heat from the blood and toxic material from the body；Root of Dahurain angelica detumescence and apocenosis；
Rhizoma typhonii whitening and moistening skin；Windproof wind-dispelling clearing damp, it is antipruritic；Radix Salviae Miltiorrhizae expands blood vessel, reduces swelling, analgesic；Mung bean remuval of damp and hot leads removing toxic substances off.
Specific embodiment 2: the present embodiment is different from the first embodiment in that, the freeze-drying of the Stem Cell Activity factor
Powder Combined with Chinese Herbal treats the preparation of light moderate acne, it is in parts by weight by 1 part of Stem Cell Activity factor freeze-dried powder, 10 parts
Honeysuckle liquid and 10 parts of mixing herb powder are made.It is other same as the specific embodiment one.
Specific embodiment 3: the present embodiment is different from the first embodiment in that, the freeze-drying of the Stem Cell Activity factor
Powder Combined with Chinese Herbal treats the preparation of light moderate acne, it is in parts by weight by 1 part of Stem Cell Activity factor freeze-dried powder, 10 parts
Honeysuckle liquid and 15 parts of mixing herb powder are made.It is other same as the specific embodiment one.
Specific embodiment 4: present embodiment Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne system
The preparation method of agent, it is sequentially included the following steps:
One, secondary culture is carried out to the mescenchymal stem cell in umbilical cord source, take P3 for mescenchymal stem cell (0.5~1) ×
106It is a；
Two, secondary culture is carried out to adipose-derived mescenchymal stem cell, take P3 for mescenchymal stem cell (0.5~1) ×
106It is a；
Three, the P3 in step 1 and step 2 is mixed for mescenchymal stem cell, is then inoculated in 20ml free serum culture
In base, then it is placed in 37 DEG C, CO2Cultivate 3~5 days in the incubator that volumetric concentration is 5%, then collection P3 is for cell supernatant,
It is sub-packed in sterile centrifugation tube, is saved at 4 DEG C；
Four, continue to cultivate according to the operating process of step 1 to step 3, collect P4, P5 and P6 respectively for cell conditioned medium
Liquid is sub-packed in sterile centrifugation tube, is saved at 4 DEG C；
Five, P3, P4, P5 and P6 are mixed for cell supernatant, by 0.22 μm of filter filtration sterilization, is protected in 4 DEG C of refrigerators
It deposits, takes mixing supernatant 10ml, 2~6g mannitol is added, is then sub-packed in cillin bottle, every bottle of 2~4ml, is pre-chilled 2 at -80 DEG C
Then~4h carries out the production of freeze-dried powder and is stored at 4 DEG C to save；
Six, 20 parts of honeysuckle is added in 100 parts of 100 DEG C of water in parts by weight, intense fire boils 30min, then literary
Fire boils 20min, then by 0.22 μm of filter filtering, is placed in the sterile liquid storage bottle of 100ml, as honeysuckle liquid, protects at 4 DEG C
It deposits；
Seven, 10 parts of radix scutellariae, 10 parts of the root of Dahurain angelica, 15 parts of rhizoma typhonii, 25 parts of radix saposhnikoviae, 8 parts of pellet are taken in parts by weight
Ginseng and 5 parts of mung bean, grind into powder after mixing are placed in sterile liquid storage bottle, are as mixed by the strainer filtering of 100 mesh
Herb powder saves in a cool and dry place, i.e. completion Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne system
The preparation of agent.
The mescenchymal stem cell in umbilical cord source described in present embodiment step 1 carries out secondary culture: in aseptic working platform
Interior, umbilical cord is handled by 75% alcohol disinfecting, then clean 2 times with physiological saline, at length is 2 by the umbilical cord scissors after cleaning
The section of~3cm, is then placed in plate, removes magnificent general formula glue, then dissect to 0.2~2mm3Bulk, then passed through at 37 DEG C
It crosses the Collagenase I that mass concentration is 0.1%~0.2% to digest 1 hour, obtains single cell suspension, count and adjust cell concentration, so
After be inoculated in sterile culture flask, be placed in 37 DEG C, CO2It is cultivated in the incubator that volumetric concentration is 5%, originally culture every 3~5 days
After change the liquid once, when cell growth fusion degree reaches 80%, cell culture fluid is collected, then 1300rpm be centrifuged 6min, take
Supernatant；Twice with normal saline flushing by solid formation, it is then digested at 37 DEG C with the pancreatin that percent by volume is 0.25%
3min is added supernatant and terminates pancreatin reaction, collect cell, is then centrifuged 6min in 1300rpm, collects cell, then connect again
Kind carries out secondary culture into another culture bottle.
Adipose-derived mescenchymal stem cell described in present embodiment step 2 carries out secondary culture: in aseptic working platform
It is interior, the adipose tissue that liposuction obtains is divided in centrifuge tube, 1300rpm is centrifuged 6min, by the bulk rouge on the upper layer after centrifugation
Fat tissue pours into another centrifuge tube, and physiological saline or PBS cleaning is added, is centrifuged 6min in 1300rpm, abandons supernatant and takes precipitating,
It cleans repeatedly in the same way 3-5 times, the Collagenase I that the mass concentration that 3 times of volumes are then added is 0.075% (takes 7.5g
Collagenase I is dissolved in the deionized water of 100ml sterilizing, and 0.22 zut filter is sub-packed in new 50ml centrifuge tube, is used
When 100 times dilution)；In 37 DEG C of water-baths, 15~30min is digested, every 5min shakes up once；After digestion, it is added and manages
The DMEM of middle liquid aliquot terminates digestion, the filtration of 200 mesh screens, the fragment tissue generated after removal digestion, then 1300rpm from
Heart 6min abandons supernatant, 20ml physiological saline is added, cell is resuspended, count；It is centrifuged 6min in 1300rpm, supernatant is abandoned and addition contains
Cell is resuspended in the DMEM culture medium of 10%FBS, presses (2~3) × 106In a cell/T75 culture bottle, and with containing 10%FBS's
Liquid in culture bottle is complemented to 11ml by DMEM, is placed in 37 DEG C, CO2It is cultivated in the incubator that volumetric concentration is 5%, originally culture
It is changed the liquid once after every 3~5 days, when cell growth fusion degree reaches 80%, collects cell renewed vaccination into another culture bottle
Carry out secondary culture.
Serum free medium purchase is from Helios company in present embodiment step 3, UltraGROTM serum substitute with
Basic base co-cultures.
The Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal prepared in present embodiment treats making for light moderate acne preparation
It is as follows with method:
1, mild acne patient:
Golden flower liquid 10ml is taken, herb powder 10g is mixed, is mixed, Chinese medicine cream is obtained；
1g freeze-dried powder is taken, is added 1ml distilled water (or mineral water), mixes, obtains cell factor liquid (pink)；
Factor liquid is embrocated and embrocates Chinese medicine cream after affected part or full face, 30min, is washed off after 20~30min with clear water, wipes face
Towel is dried, then full face embrocates cell factor liquid；
2, middle severe acne patient:
Golden flower liquid 10ml is taken, herb powder 15g is mixed, mixes, obtains traditional Chinese medicine emulsifiable paste；
1g freeze-dried powder is taken, is added 1ml distilled water (or mineral water), mixes, obtains cell factor liquid (pink)；
Factor liquid is embrocated and embrocates Chinese medicine cream after affected part or full face, 30min, is washed off after 20~30min with clear water, wipes face
Towel is dried, then full face embrocates cell factor liquid.
Specific embodiment 5: present embodiment is unlike specific embodiment four, to umbilical cord source in step 1
Mescenchymal stem cell carry out secondary culture, take P3 for mescenchymal stem cell 0.8 × 106It is a.Other steps and parameter and specific
Embodiment four is identical.
Specific embodiment 6: present embodiment is unlike specific embodiment four, to adipose-derived in step 2
Mescenchymal stem cell carry out secondary culture, take P3 for mescenchymal stem cell 0.8 × 106It is a.Other steps and parameter and specific
Embodiment four is identical.
Specific embodiment 7: present embodiment is unlike specific embodiment four, according to step 1 in step 4
To step 3 operating process continue culture after, take P6 to carry out virus, immunophenotype and Cell viability for mescenchymal stem cell
Detection, it is spare after indices are qualified；The qualification of index refers to: viral diagnosis is feminine gender, and immunophenotype positive expression rate exists
95% or more, negative expression rate is lower than 2%, and Cell viability is at least 95%.Other steps and parameter and specific embodiment four
It is identical.
Specific embodiment 8: present embodiment is unlike specific embodiment four, according to step 1 in step 4
To step 3 operating process continue culture after, take P6 to carry out sterile and cytokine content detection for cell supernatant,
It is spare after indices are qualified；The qualification of index refers to: Bacteria Detection is feminine gender, and it is raw that cytokines measurement goes out to contain blood vessel endothelium
The long factor (VEGF), epidermal growth factor (EGF), nerve cell growth factor (NGF), transforming growth factor (TGF-β), blood are small
Plate source growth factor (PDGF) and basic fibroblast growth factor (bFGF).Other steps and parameter and specific embodiment
Four is identical.
Specific embodiment 9: present embodiment takes mixing supernatant unlike specific embodiment four, in step 5
10ml is added 4-6g mannitol, is then sub-packed in cillin bottle, every bottle of 2ml, 3h is pre-chilled at -80 DEG C.Other steps and parameter with
Specific embodiment four is identical.
Beneficial effects of the present invention are verified by following embodiment:
Embodiment
Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation method of light moderate acne preparation, it is according to the following steps
It carries out:
One, secondary culture is carried out to the mescenchymal stem cell in umbilical cord source, takes P3 for mescenchymal stem cell 0.8 × 106It is a；
Two, secondary culture is carried out to adipose-derived mescenchymal stem cell, takes P3 for mescenchymal stem cell 0.8 × 106It is a；
Three, the P3 in step 1 and step 2 is mixed for mescenchymal stem cell, is then inoculated in 20ml free serum culture
In base, then it is placed in 37 DEG C, CO2It is cultivated in the incubator that volumetric concentration is 5% 4 days, then collects P3 for cell supernatant, packing
In in sterile centrifugation tube, saved at 4 DEG C；
Four, continue to cultivate according to the operating process of step 1 to step 3, collect P4, P5 and P6 respectively for cell conditioned medium
Liquid is sub-packed in sterile centrifugation tube, is saved at 4 DEG C；
Five, P3, P4, P5 and P6 are mixed for cell supernatant, by 0.22 μm of filter filtration sterilization, is protected in 4 DEG C of refrigerators
It deposits, takes mixing supernatant 10ml, 4g mannitol is added, is then sub-packed in cillin bottle, every bottle of 2ml, 3h is pre-chilled at -80 DEG C, then
It carries out the production of freeze-dried powder and is stored at 4 DEG C to save；
Six, 20 parts of honeysuckle is added in 100 parts of 100 DEG C of water in parts by weight, intense fire boils 30min, then literary
Fire boils 20min, then by 0.22 μm of filter filtering, is placed in the sterile liquid storage bottle of 100ml, as honeysuckle liquid, protects at 4 DEG C
It deposits；
Seven, 10 parts of radix scutellariae, 10 parts of the root of Dahurain angelica, 15 parts of rhizoma typhonii, 25 parts of radix saposhnikoviae, 8 parts of pellet are taken in parts by weight
Ginseng and 5 parts of mung bean, grind into powder after mixing are placed in sterile liquid storage bottle, are as mixed by the strainer filtering of 100 mesh
Herb powder saves in a cool and dry place, i.e. completion Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne system
The preparation of agent.
The mescenchymal stem cell in umbilical cord source described in the present embodiment step 1 carries out secondary culture: in aseptic working platform
Interior, umbilical cord is handled by 75% alcohol disinfecting, then clean 2 times with physiological saline, at length is 2 by the umbilical cord scissors after cleaning
The section of~3cm, is then placed in plate, removes magnificent general formula glue, then dissect to 0.2~2mm3Bulk, then passed through at 37 DEG C
It crosses the Collagenase I that mass concentration is 0.1%~0.2% to digest 1 hour, obtains single cell suspension, count and adjust cell concentration, so
After be inoculated in sterile culture flask, be placed in 37 DEG C, CO2It is cultivated in the incubator that volumetric concentration is 5%, originally culture every 3~5 days
After change the liquid once, when cell growth fusion degree reaches 80%, cell culture fluid is collected, then 1300rpm be centrifuged 6min, take
Supernatant；Twice with normal saline flushing by solid formation, it is then digested at 37 DEG C with the pancreatin that percent by volume is 0.25%
3min is added supernatant and terminates pancreatin reaction, collect cell, is then centrifuged 6min in 1300rpm, collects cell, then connect again
Kind carries out secondary culture into another culture bottle.
Adipose-derived mescenchymal stem cell described in the present embodiment step 2 carries out secondary culture: in aseptic working platform
It is interior, the adipose tissue that liposuction obtains is divided in centrifuge tube, 1300rpm is centrifuged 6min, by the bulk rouge on the upper layer after centrifugation
Fat tissue pours into another centrifuge tube, and physiological saline or PBS cleaning is added, is centrifuged 6min in 1300rpm, abandons supernatant and takes precipitating,
It cleans repeatedly in the same way 3-5 times, the Collagenase I that the mass concentration that 3 times of volumes are then added is 0.075% (takes 7.5g
Collagenase I is dissolved in the deionized water of 100ml sterilizing, and 0.22 zut filter is sub-packed in new 50ml centrifuge tube, is used
When 100 times dilution)；In 37 DEG C of water-baths, 15~30min is digested, every 5min shakes up once；After digestion, it is added and manages
The DMEM of middle liquid aliquot terminates digestion, the filtration of 200 mesh screens, the fragment tissue generated after removal digestion, then 1300rpm from
Heart 6min abandons supernatant, 20ml physiological saline is added, cell is resuspended, count；It is centrifuged 6min in 1300rpm, supernatant is abandoned and addition contains
Cell is resuspended in the DMEM culture medium of 10%FBS, presses (2~3) × 106In a cell/T75 culture bottle, and with containing 10%FBS's
Liquid in culture bottle is complemented to 11ml by DMEM, is placed in 37 DEG C, CO2It is cultivated in the incubator that volumetric concentration is 5%, originally culture
It is changed the liquid once after every 3~5 days, when cell growth fusion degree reaches 80%, collects cell renewed vaccination into another culture bottle
Carry out secondary culture.
Serum free medium is bought from Helios company, UltraGROTM serum substitute and base in the present embodiment step 3
Plinth base co-cultures.
After continuing culture according to the operating process of step 1 to step 3 in the present embodiment step 4, take P6 for mesenchyma
Stem cell carries out the detection of virus, immunophenotype and Cell viability, spare after indices are qualified；The qualification of index refers to: disease
Poison is detected as feminine gender, and immunophenotype positive expression rate is 95% or more, and negative expression rate is lower than 2%, and Cell viability is at least
95%.
After continuing culture according to the operating process of step 1 to step 3 in the present embodiment step 4, take P6 on cell
Clear liquid carries out sterile and cytokine content detection, spare after indices are qualified；The qualification of index refers to: Bacteria Detection is
Feminine gender, cytokines measurement, which goes out, contains vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), nerve growth
The factor (NGF), transforming growth factor (TGF-β), platelet derived growth factor (PDGF) and basic fibroblast growth factor
(bFGF), specific as shown in table 1, it is seen that active factors type is more, and content is high；These factors are activity polypeptid substances, strong to promote
Into the proliferation of human skin cell, growth, safely, effectively, can quickly repair damaged skin, and improvement property skin reparation,
Nursing.
The detection (unit: ng/ml) of the chief active factor content of 1 mescenchymal stem cell of table secretion
The Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal prepared in embodiment treats the user of light moderate acne preparation
Method is as follows:
1, mild acne patient:
Golden flower liquid 10ml is taken, herb powder 10g is mixed, is mixed, Chinese medicine cream is obtained；
1g freeze-dried powder is taken, is added 1ml distilled water (or mineral water), mixes, obtains cell factor liquid (pink)；
Factor liquid is embrocated and embrocates Chinese medicine cream after affected part or full face, 30min, is washed off after 20~30min with clear water, wipes face
Towel is dried, then full face embrocates cell factor liquid；
2, middle severe acne patient:
Golden flower liquid 10ml is taken, herb powder 15g is mixed, is mixed, Chinese medicine cream is obtained；
1g freeze-dried powder is taken, is added 1ml distilled water (or mineral water), mixes, obtains cell factor liquid (pink)；
Factor liquid is embrocated and embrocates Chinese medicine cream after affected part or full face, 30min, is washed off after 20~30min with clear water, wipes face
Towel is dried, then full face embrocates cell factor liquid.
Cytokine profiles are all related with acne, including sIL-2R, TNF-α, IL-1, IL-6, IL-8, α-MSH are trained in vitro
The expression of proinflammatory inflammation factor can obviously be raised by supporting discovery lipopolysaccharides (LPS) in cortex cell research, and lipopolysaccharides can stimulate cortex thin
The release of the cytoplasmic matrix cell source factor (CXCL8), TNF-α and IL-1 α, P.acnes can stimulate the release of CXCL8 and TNF-α.
In the inflammation mechanism of acne, α-MSH (α melanotropin) is considered as a maincenter proinflammatory mediators.On sebaceous cell surface
It was found that energy expression activity platelet activity factor receptor (PAF-R), PAF-R is related with inflammatory mediator expression is adjusted, including epoxy
Synthase 2, prostaglandin E and IL-8.
According to above situation, gained Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treatment is prepared in the present embodiment to using
Light moderate acne preparation (for A group), to newborn (for B group) and unused (blank group) using only the Chinese medicine prepared in the present embodiment
Compare test；Detect face by VISIA skin detection instrument；
Blank group: it is detected before use；
A group: right face uses；
B group: left face uses.
The discovery of inflammatory factor Concentration Testing, Stem Cell Activity in the present embodiment are carried out to Skin tissue fluid before and after acne treatment
Factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne preparation, in the significant effect for treating light moderate acne is higher than and is used alone
The effect of medicine preparation for treating, the results are shown in Table 2, it is seen that pro-inflammatory cytokine concentration significantly reduces, and effect is more significant.
Pro-inflammatory cytokine concentration situation of change after 2 stem cell factor Combined with Chinese Herbal of table and the light moderate acne of Chinese medicine preparation treatment
VISIA skin detection instrument red sector index analysis, the results are shown in Table 3, the red sector scoring of patient after the treatment of A group
It is decreased obviously than B group and blank group, illustrates that the facial inflammatory conditions of patient after A group is treated significantly improve.Meanwhile passing through VISIA
The numerical value that the comparison of red sector percentage can be seen that patient's red sector after the treatment of A group is significantly improved compared with other two groups, illustrates patient
Facial inflammatory conditions have defeated more same age groups；(percentage, which represents, chooses 100 agnate, other contemporarys of the same sex
It is detected, test result is assumed to be 90%, then just illustrating that testing result has been more than 90% crowd, then percentage gets over Gao Yue
It is good；Scoring represents the spot of skin surface, and wrinkle, acne hole, acne print, how much equal number of pore is, thus numerical value it is more low more
It is good).
Face's inflammation improves situation after 3 stem cell factor Combined with Chinese Herbal of table treats light moderate acne
By using the pretherapy and post-treatment acne acne print situation of change of VISIA detection face, as illustrated in fig. 1 and 2, stem cell factor
Freeze-dried powder Combined with Chinese Herbal uses, and can significantly dispel acne print.
It is detected simultaneously by VISIA eye wrinkle-removing, the results are shown in Table 4, and the wrinkle scoring of A group is more aobvious than B group and blank group
Writing reduces, and illustrates that a situation arises is obviously improved for patient's wrinkle after A group is treated.The wrinkle percentage result that same age group compares can
To find out, A group percentages are significantly higher than other two groups, illustrate that the wrinkle of patient after A group is treated is better than more same age groups
Crowd.
4 stem cell factor Combined with Chinese Herbal of table treats the improvement to eye fine wrinkles
By using VISIA detection face, eye wrinkle-removing situation of change as shown in Figures 3 and 4, can after treatment before and after treatment
The reduction for seeing apparent deep wrinkles and fine wrinkles illustrates that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal is controlled in the present embodiment
Treating light moderate acne preparation helps to reduce fine wrinkles.
Claims (9)
1. the preparation that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne, it is characterised in that it is in parts by weight
It is made of 1~2 part of Stem Cell Activity factor freeze-dried powder, 8~12 parts of honeysuckle liquid and 8~20 parts of mixing herb powder；
Wherein the mixing herb powder is in parts by weight by 10 parts of radix scutellariae, 10 parts of the root of Dahurain angelica, 15 parts of rhizoma typhonii, 25 parts anti-
Wind, 8 parts of Radix Salviae Miltiorrhizae and 5 parts of mung bean are made.
2. Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal according to claim 1 treats the preparation of light moderate acne,
It is characterized in that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats that the preparation of light moderate acne, it is in parts by weight by 1 part
Stem Cell Activity factor freeze-dried powder, 10 parts of honeysuckle liquid and 10 parts of mixing herb powder are made.
3. Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal according to claim 1 treats the preparation of light moderate acne,
It is characterized in that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats that the preparation of light moderate acne, it is in parts by weight by 1 part
Stem Cell Activity factor freeze-dried powder, 10 parts of honeysuckle liquid and 15 parts of mixing herb powder are made.
4. preparing the side that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal as described in claim 1 treats the preparation of light moderate acne
Method, it is characterised in that it is sequentially included the following steps:
One, secondary culture is carried out to the mescenchymal stem cell in umbilical cord source, takes P3 for mescenchymal stem cell (0.5~1) × 106It is a；
Two, secondary culture is carried out to adipose-derived mescenchymal stem cell, takes P3 for mescenchymal stem cell (0.5~1) × 106It is a；
Three, the P3 in step 1 and step 2 is mixed for mescenchymal stem cell, is then inoculated in 20ml serum free medium,
It is placed in 37 DEG C, CO again2It is cultivated in the incubator that volumetric concentration is 5% 3~5 days, then collects P3 for cell supernatant, be sub-packed in
In sterile centrifugation tube, saved at 4 DEG C；
Four, continue to cultivate according to the operating process of step 1 to step 3, collect P4, P5 and P6 respectively for cell supernatant, divide
Loaded in sterile centrifugation tube, saved at 4 DEG C；
Five, P3, P4, P5 and P6 are mixed for cell supernatant, by 0.22 μm of filter filtration sterilization, saves, take in 4 DEG C of refrigerators
Supernatant 10ml is mixed, 2~6g mannitol is added, is then sub-packed in cillin bottle, every bottle of 2~4ml, 2~4h is pre-chilled at -80 DEG C,
Then it carries out the production of freeze-dried powder and is stored at 4 DEG C to save；
Six, 20 parts of honeysuckle is added in 100 parts of 100 DEG C of water in parts by weight, intense fire boils 30min, is then cooked by slow fire
20min, then by 0.22 μm of filter filtering, be placed in the sterile liquid storage bottle of 100ml, as honeysuckle liquid, saved at 4 DEG C；
Seven, 10 parts of radix scutellariae, 10 parts of the root of Dahurain angelica, 15 parts of rhizoma typhonii, 25 parts of radix saposhnikoviae, 8 parts of Radix Salviae Miltiorrhizae and 5 are taken in parts by weight
The mung bean of part, grind into powder after mixing are placed in sterile liquid storage bottle by the strainer filtering of 100 mesh, as mixing traditional Chinese medicine powder
End saves in a cool and dry place, that is, completes the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats light moderate acne preparation
It is standby.
5. the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal according to claim 4 treats the preparation of light moderate acne
Preparation Method, it is characterised in that secondary culture is carried out to the mescenchymal stem cell in umbilical cord source in step 1, takes P3 dry for mesenchyma
Cell 0.8 × 106It is a.
6. the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal according to claim 4 treats the preparation of light moderate acne
Preparation Method, it is characterised in that secondary culture is carried out to adipose-derived mescenchymal stem cell in step 2, takes P3 dry for mesenchyma
Cell 0.8 × 106It is a.
7. the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal according to claim 4 treats the preparation of light moderate acne
Preparation Method, it is characterised in that after continuing culture according to the operating process of step 1 to step 3 in step 4, P6 is taken to fill between
Matter stem cell carries out the detection of virus, immunophenotype and Cell viability, spare after indices are qualified；The qualification of index refers to:
Viral diagnosis is feminine gender, and immunophenotype positive expression rate is 95% or more, and negative expression rate is lower than 2%, and Cell viability is at least
95%.
8. the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal according to claim 4 treats the preparation of light moderate acne
Preparation Method, it is characterised in that after continuing culture according to the operating process of step 1 to step 3 in step 4, take P6 for cell
Supernatant carries out sterile and cytokine content detection, spare after indices are qualified；The qualification of index refers to: Bacteria Detection
For feminine gender, cytokines measurement, which goes out, contains vascular endothelial growth factor, epidermal growth factor, nerve cell growth factor, conversion
Growth factor, platelet derived growth factor and basic fibroblast growth factor.
9. the system that Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal according to claim 4 treats the preparation of light moderate acne
Preparation Method, it is characterised in that take mixing supernatant 10ml in step 5,4-6g mannitol is added, is then sub-packed in cillin bottle, often
Bottle 2ml, is pre-chilled 3h at -80 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910816379.6A CN110393726A (en) | 2019-08-30 | 2019-08-30 | Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation and preparation method thereof of light moderate acne |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910816379.6A CN110393726A (en) | 2019-08-30 | 2019-08-30 | Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation and preparation method thereof of light moderate acne |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110393726A true CN110393726A (en) | 2019-11-01 |
Family
ID=68329655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910816379.6A Pending CN110393726A (en) | 2019-08-30 | 2019-08-30 | Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation and preparation method thereof of light moderate acne |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110393726A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111743685A (en) * | 2020-06-24 | 2020-10-09 | 天晴干细胞股份有限公司 | Medical cold compress patch and preparation method thereof |
| CN112515999A (en) * | 2020-12-21 | 2021-03-19 | 阿谷巴(杭州)生物科技发展有限公司 | Mite and acne removing composition rich in cytokines and preparation method thereof |
| CN116392575A (en) * | 2023-03-22 | 2023-07-07 | 广东唯泰生物科技有限公司 | Preparation for treating acne by combining stem cell active ingredients with hyaluronic acid |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104922658A (en) * | 2015-07-16 | 2015-09-23 | 广州赛莱拉干细胞科技股份有限公司 | Composition with anti-aging function and preparation thereof |
| CN105997771A (en) * | 2016-08-01 | 2016-10-12 | 广州赛莱拉干细胞科技股份有限公司 | Inflammation diminishing and acne removing mask and preparation method thereof |
| CN106367386A (en) * | 2016-10-14 | 2017-02-01 | 中卫华医（北京）生物科技有限公司 | Preparation method of human umbilical cord mesenchymal stem cell factor freeze-dried powder |
| CN108057014A (en) * | 2017-12-25 | 2018-05-22 | 北京汇智驰康生物科技有限公司 | A kind of preparation method of the stem cell medicine of beauty and skin care |
| CN108272643A (en) * | 2018-02-01 | 2018-07-13 | 伯仕利生物科技发展（盐城）有限公司 | A kind of preparation method for the compound freeze-dried powder of multiple stem cell and solvent |
| CN108969466A (en) * | 2018-10-12 | 2018-12-11 | 青岛西凯生物技术有限公司 | A kind of facial mask and preparation method thereof of preserving youthful looks based on the umbilical cord mesenchymal stem cells secretion activity factor |
-
2019
- 2019-08-30 CN CN201910816379.6A patent/CN110393726A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104922658A (en) * | 2015-07-16 | 2015-09-23 | 广州赛莱拉干细胞科技股份有限公司 | Composition with anti-aging function and preparation thereof |
| CN105997771A (en) * | 2016-08-01 | 2016-10-12 | 广州赛莱拉干细胞科技股份有限公司 | Inflammation diminishing and acne removing mask and preparation method thereof |
| CN106367386A (en) * | 2016-10-14 | 2017-02-01 | 中卫华医（北京）生物科技有限公司 | Preparation method of human umbilical cord mesenchymal stem cell factor freeze-dried powder |
| CN108057014A (en) * | 2017-12-25 | 2018-05-22 | 北京汇智驰康生物科技有限公司 | A kind of preparation method of the stem cell medicine of beauty and skin care |
| CN108272643A (en) * | 2018-02-01 | 2018-07-13 | 伯仕利生物科技发展（盐城）有限公司 | A kind of preparation method for the compound freeze-dried powder of multiple stem cell and solvent |
| CN108969466A (en) * | 2018-10-12 | 2018-12-11 | 青岛西凯生物技术有限公司 | A kind of facial mask and preparation method thereof of preserving youthful looks based on the umbilical cord mesenchymal stem cells secretion activity factor |
Non-Patent Citations (2)
| Title |
|---|
| 刘子君: "治疗痤疮外用中药专利用药规律分析", 《新中医》, vol. 50, no. 10 * |
| 刘子君等: "治疗痤疮外用中药专利用药规律分析", 《新中医》, vol. 50, no. 10, 31 October 2018 (2018-10-31) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111743685A (en) * | 2020-06-24 | 2020-10-09 | 天晴干细胞股份有限公司 | Medical cold compress patch and preparation method thereof |
| CN111743685B (en) * | 2020-06-24 | 2022-07-15 | 天晴干细胞股份有限公司 | Medical cold compress subsides |
| CN112515999A (en) * | 2020-12-21 | 2021-03-19 | 阿谷巴(杭州)生物科技发展有限公司 | Mite and acne removing composition rich in cytokines and preparation method thereof |
| CN116392575A (en) * | 2023-03-22 | 2023-07-07 | 广东唯泰生物科技有限公司 | Preparation for treating acne by combining stem cell active ingredients with hyaluronic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110393726A (en) | Stem Cell Activity factor freeze-dried powder Combined with Chinese Herbal treats the preparation and preparation method thereof of light moderate acne | |
| CN108309822A (en) | A kind of preparation method of human umbilical cord mesenchymal stem cells paracrine factor freeze-dried powder | |
| CN104958319A (en) | Mesenchymal stem cell and cytokine preparation having treatment effects on premature ovarian failures and perimenopausal syndromes, and preparing method for preparation | |
| CN102586182A (en) | Preparation and application of human-stem-cell-secreted bioactive factor and lysis solution | |
| CN106367389A (en) | Preparation method and application of human umbilical cord mesenchymal stem cell factors | |
| CN108392494A (en) | Applications of the exosome of people's umbilical cord mesenchymal stem cells secretion in preparing the drug for repairing skin ultraviolet injury | |
| CN108524601A (en) | A kind of vaginal jellies and preparation method thereof containing pleiotrophic factor | |
| CN108721606A (en) | A kind of striae of pregnancy reparation product and preparation method thereof based on stem cell factor | |
| CN106566803A (en) | Culture solution, application of culture solution and method for culturing umbilical cord mesenchymal stem cells | |
| CN109876189A (en) | A method of fat source biomaterial is prepared using ultrasonic high-efficiency | |
| CN109985064A (en) | Mescenchymal stem cell secretes the purposes of extract, mescenchymal stem cell secretion extract and preparation method thereof | |
| Nalisa et al. | Stem cell therapy for diabetic foot ulcers: Theory and practice | |
| CN107779430A (en) | The collection method of umbilical cord mesenchymal stem cells supernatant | |
| CN112322681B (en) | Traditional Chinese medicine maggot active peptide and preparation method and application thereof | |
| CN111568851A (en) | Method for producing active factors by using perinatal MSC and cosmetic preparation | |
| CN111172103A (en) | Preparation method and application of stem cell exosome stimulated by angelica sinensis extract | |
| CN106333965A (en) | Preparation for treating osteoarthritis and treatment method | |
| CN207452106U (en) | For the fat-derived primary stem cell collection piece-rate system of binary channels of human body liposuction | |
| CN110526958B (en) | Activity repair peptide Tvigour A and application thereof | |
| He et al. | Human menstrual blood-derived stem cells combined with a new 3D bioprinted composite scaffold for spinal cord injury treatment | |
| CN108143748A (en) | A kind of dog MSC excretion body preparations and preparation method thereof | |
| CN108324737B (en) | Combination of umbilical cord mesenchymal stem cells for treating hyperglycemia and diabetic nephropathy | |
| CN109010368B (en) | Cell preparation for treating triple sign of female athletes and repairing female athlete ovary function and preparation method thereof | |
| CN117106712B (en) | Stem cell exosome for skin regeneration, application and product thereof | |
| CN109929801A (en) | A kind of induction people's umbilical cord Wharton jelly mescenchymal stem cell is to nucleus pulposus cell differentiation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Lingjuan Inventor after: Zhang Yi Inventor after: Zhang Feiran Inventor after: Liu Yanqing Inventor before: Wang Lingjuan Inventor before: Zhang Yi Inventor before: Liu Yanqing |
|
| CB03 | Change of inventor or designer information |