CN110386976A - A kind of grass carp air bladder collagen self-assembling method and collagen biomaterial - Google Patents
A kind of grass carp air bladder collagen self-assembling method and collagen biomaterial Download PDFInfo
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- CN110386976A CN110386976A CN201910553200.2A CN201910553200A CN110386976A CN 110386976 A CN110386976 A CN 110386976A CN 201910553200 A CN201910553200 A CN 201910553200A CN 110386976 A CN110386976 A CN 110386976A
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- 241000252230 Ctenopharyngodon idella Species 0.000 title claims abstract description 125
- 102000008186 Collagen Human genes 0.000 title claims abstract description 75
- 108010035532 Collagen Proteins 0.000 title claims abstract description 75
- 229920001436 collagen Polymers 0.000 title claims abstract description 75
- 210000004712 air sac Anatomy 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000012620 biological material Substances 0.000 title abstract description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 48
- 239000011780 sodium chloride Substances 0.000 claims abstract description 24
- 238000006243 chemical reaction Methods 0.000 claims abstract description 22
- 238000000605 extraction Methods 0.000 claims abstract description 5
- 239000000047 product Substances 0.000 claims description 28
- 230000001376 precipitating effect Effects 0.000 claims description 20
- 238000001816 cooling Methods 0.000 claims description 16
- 239000004365 Protease Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 10
- 229940088598 enzyme Drugs 0.000 claims description 10
- 235000019419 proteases Nutrition 0.000 claims description 10
- 238000005119 centrifugation Methods 0.000 claims description 8
- 241000251468 Actinopterygii Species 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 6
- 238000005238 degreasing Methods 0.000 claims description 6
- 238000004090 dissolution Methods 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 108090000526 Papain Proteins 0.000 claims description 5
- 102000057297 Pepsin A Human genes 0.000 claims description 5
- 108090000284 Pepsin A Proteins 0.000 claims description 5
- 239000003513 alkali Substances 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 230000009849 deactivation Effects 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- 229940111202 pepsin Drugs 0.000 claims description 5
- 238000000502 dialysis Methods 0.000 claims description 4
- 238000005360 mashing Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 229960000583 acetic acid Drugs 0.000 claims 1
- 238000004108 freeze drying Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000001338 self-assembly Methods 0.000 abstract description 21
- 239000004833 fish glue Substances 0.000 abstract description 6
- 238000002835 absorbance Methods 0.000 description 6
- 238000011161 development Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000005057 refrigeration Methods 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000005660 chlorination reaction Methods 0.000 description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000002473 artificial blood Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 235000019628 coolness Nutrition 0.000 description 1
- 235000006694 eating habits Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 108010059642 isinglass Proteins 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses a kind of grass carp air bladder collagen self-assembling method and collagen biomaterial, white self-assembling method obtains grass carp air bladder collagen the following steps are included: extracting from grass carp；It is extracted from grass carp and obtains grass carp fribrillin；The grass carp fribrillin of extraction is digested, grass carp fribrillin enzymolysis product is obtained；Grass carp air bladder collagen albumen acetic solution is configured, under conditions of 25-40 DEG C, pH 6.0-7.0, sodium chloride solution is added, with grass carp fribrillin enzymolysis product, carries out grass carp air bladder collagen self-assembling reaction.Grass carp fish glue original albumen self assembly in the present invention promotes grass carp air bladder collagen self assembly using grass carp fribrillin enzymolysis product, keeps its space structure more fine and close, improves the performance of grass carp air bladder collagen.
Description
Technical field
The invention belongs to biological food technical fields, and in particular to a kind of grass carp air bladder collagen self-assembling method and glue
Former protein biology raw material.
Background technique
Grass carp is first of four large Chinese carp of China, because the advantages that its high protein, low fat, big quantity of the catch will be widely welcomed.
But in the using and process of grass carp, due to processing technology is relatively backward and eating habit etc., air bladder, fish-skin, fish scale etc.
It is not fully utilized, through being abandoned frequently as leftover bits and pieces.Collagen is extracted from grass carp air bladder had both reduced money
Source waste increases economic benefit again.
Collagen (collagen) is a kind of fibrous structure protein being widely present in organism, according to its point
The difference of minor structure can be divided into the multiple types such as I, II, III, and wherein Type I collagen albumen is that most important in organism there are shapes
Formula, structure feature are the triple-helix structures formed by two α 1 and 2 peptide chain of α.Since collagen has good life
Object compatibility, flexibility (adaptability) of operation and low immunological rejection, it is as the primary biological material of medical tissue engineering in people in recent years
The fields such as skin, artificial blood vessel, wound repair are made to be widely used.
Collagen is self-assembly of the reticular structure of high-sequential in vitro, is conducive to sticking, be proliferated, spreading for cell
And migration, there is good biocompatibility, excellent mechanical property, biodegradable and poor antigen.In recent years, it designs
With synthesis there are the collagen self assembly Biofunctional materials of excellent properties to have become research hotspot.However at present about grass carp
The research of air bladder collagen self assembly behavior does not have been reported that also.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of grass carp air bladder collagen self-assembling methods, improve collagen
The performance of albumen.
The technical solution of the present invention is as follows: a kind of grass carp air bladder collagen self-assembling method, comprising the following steps:
(1) it is extracted from grass carp and obtains grass carp air bladder collagen；
(2) it is extracted from grass carp and obtains grass carp fribrillin；
(3) the grass carp fribrillin of extraction is digested, obtains grass carp fribrillin enzymolysis product；
(4) grass carp air bladder collagen albumen acetic solution is configured, under conditions of 25-40 DEG C, pH 6.0-7.0, chlorination is added
Sodium solution carries out grass carp air bladder collagen self-assembling reaction with grass carp fribrillin enzymolysis product.
Preferably, the concentration of the grass carp air bladder collagen albumen acetic solution is 1.0-4.0mg/mL.
Preferably, the concentration of the fribrillin enzymolysis product is 0.5-2.0mg/mL.
Preferably, the concentration of the sodium chloride solution is 50-100mmol/mL.
Preferably, the step of extracting grass carp air bladder collagen in the step (1) includes:
It will sufficiently be washed to neutrality after grass carp air bladder degreasing, extractant is added and reacts 24-48h, supernatant is added for centrifugation
Sodium chloride is pre-chilled to saltout, stands, then centrifuging and taking precipitating, supernatant repeat operation of saltouing, and merge precipitating acetate dissolution, dialyse,
Preservation is stand-by after vacuum freeze drying.Detailed process are as follows: be sufficiently washed to neutrality after grass carp air bladder degreasing, 0.5M acetic acid is added
(1:10, w/v), 0.1% (w/v) 3000U/mg pepsin reacts 24-48h, is centrifuged 20min, supernatant under 5000r/min
Pre-cooling sodium chloride is added to saltout, stands 4h, then centrifuging and taking precipitating, supernatant repeat operation of saltouing, and merge 10 times of volumes of precipitating
0.5M acetate dissolution is dialysed, and -20 DEG C of preservations are stand-by after vacuum freeze drying.
Preferably, the step of extracting acquisition grass carp fribrillin in the step (2) includes:
After the grass carp flesh of fish is minced, pre-cooling sodium chloride solution is added and is homogenized, is placed in ice bath and stirs, filter, from
After the heart, precipitating is taken to add the homogenate of pre-cooling sodium chloride solution, centrifugation, wash cycles, gained precipitating is grass carp muscle fibril
Albumen.
Detailed process are as follows: after the grass carp flesh of fish minces, it is even that 10 times of volume pre-cooling sodium chloride solution (60mmol/L) progress are added
Slurry, is placed in ice bath and stirs 30min, filtered through gauze, is centrifuged 15min under 8000r/min, precipitating is taken to add pre-cooling sodium chloride
Solution homogenate, centrifugation, wash cycles 3 times, gained precipitating is grass carp fribrillin.
Preferably, the step of grass carp fribrillin digests in the step (3) includes:
A certain amount of fribrillin is taken, the water mashing of 3 times of weight is added, adjusts the temperature to 50 DEG C and pH 8.0, adds
Enter 3000-5000U/g compound protease to be hydrolyzed, complex enzyme ratio is Chymetin: alkali protease: papain
=1:1:0.5, boiling water bath enzyme deactivation 10min terminates reaction after reaction, and flowing water is cooled to room temperature, is placed in 0 DEG C of refrigerator and saves
Spare, gained is grass carp fribrillin enzymolysis product.
A kind of collagen biomaterial obtained by above-mentioned grass carp air bladder collagen self-assembling method.The present invention
Obtained collagen biomaterial can be applied to multiple product preparation in the prior art.
Compared with prior art, the beneficial effects of the present invention are embodied in:
Grass carp fish glue original albumen self assembly in the present invention promotes grass carp using grass carp fribrillin enzymolysis product
Air bladder collagen self assembly, keeps its space structure more fine and close, improves the performance of grass carp air bladder collagen.
Detailed description of the invention
Fig. 1 is the self assembly behavior of grass carp fish glue original albumen in embodiment 1.
Fig. 2 is the self assembly behavior of grass carp fish glue original albumen in embodiment 2.
Fig. 3 is the self assembly behavior of grass carp fish glue original albumen in embodiment 3.
Specific embodiment
Embodiment 1
A kind of grass carp air bladder collagen self-assembling method, comprising the following steps:
(1) it is sufficiently washed to neutrality after the degreasing of grass carp air bladder, 0.5M acetic acid (1:10, w/v), 0.1% (w/v) is added
3000U/mg pepsin reacts for 24 hours, and 20min is centrifuged under 5000r/min, and supernatant is added pre-cooling sodium chloride and saltouts, and stands 4h,
Centrifuging and taking precipitates again, and supernatant repeats operation of saltouing, and merges 10 times of volume 0.5M acetate dissolutions of precipitating, dialysis, vacuum refrigeration
- 20 DEG C of preservations are stand-by after drying, obtain the grass carp air bladder collagen.
(2) after the grass carp flesh of fish minces, 10 times of volume pre-coolings sodium chloride solution (50mmol/L) is added and are homogenized, are placed in
Stir 30min in ice bath, filtered through gauze is centrifuged 15min under 8000r/min, take precipitating add the homogenate of pre-cooling sodium chloride solution,
Centrifugation, wash cycles 3 times, obtains the grass carp fribrillin.
(3) 40mg/mL grass carp fribrillin acetum is prepared, under the conditions of 50 DEG C, pH8.0,3000U/g is added
Compound protease is hydrolyzed, and complex enzyme ratio is Chymetin: alkali protease: papain=1:1:0.5, reaction
After boiling water bath enzyme deactivation 10min terminate reaction, flowing water is cooled to room temperature, is placed in 0 DEG C of refrigerator and saves backup, obtain the grass
Fish fribrillin enzymolysis product.
(4) 1.0mg/mL collagen acetum is prepared, under the conditions of 25 DEG C, 6.0 pH, 50mmol/mL chlorination is added
Sodium solution carries out grass carp air bladder collagen self-assembling reaction with 0.5mg/mL grass carp fribrillin enzymolysis product.
The absorbance value at 313nm is tied up to using spectrophotometer real-time monitoring collagen self-assembly, is drawn from group
Fill curve.Wherein, the collagen self-assembling reaction for being not added with grass carp fribrillin enzymolysis product is control group, addition
The collagen self-assembling reaction of 0.5mg/mL grass carp fribrillin enzymolysis product is experimental group, the result is shown in Figure 1.
As shown in figure, compared with the control group, grass carp air bladder collagen after grass carp fribrillin enzymolysis product is added
Lag phase extend, the absorbance value of the stage of stable development increases, this illustrates that the tridimensional network for the collagenous fibres to be formed is the finest and close,
Addition grass carp fribrillin enzymolysis product can promote the self assembly of grass carp air bladder collagen, show the method for the present embodiment
Obtained grass carp air bladder collagen self assembly effect is preferable.
Embodiment 2
A kind of grass carp air bladder collagen self-assembling method, comprising the following steps:
(1) it is sufficiently washed to neutrality after the degreasing of grass carp air bladder, 0.5M acetic acid (1:10, w/v), 0.1% (w/v) is added
3000U/mg pepsin reacts 36h, and 20min is centrifuged under 5000r/min, and supernatant is added pre-cooling sodium chloride and saltouts, and stands 4h,
Centrifuging and taking precipitates again, and supernatant repeats operation of saltouing, and merges 10 times of volume 0.5M acetate dissolutions of precipitating, dialysis, vacuum refrigeration
- 20 DEG C of preservations are stand-by after drying.
(2) after the grass carp flesh of fish minces, 10 times of volume pre-cooling sodium chloride solutions the extraction of grass carp fribrillin: are added
(60mmol/L) is homogenized, and is placed in ice bath and is stirred 30min, filtered through gauze, is centrifuged 15min under 8000r/min, takes precipitating
The homogenate of pre-cooling sodium chloride solution, centrifugation are added, wash cycles 3 times, gained precipitating is grass carp fribrillin.
(3) enzymatic hydrolysis of grass carp fribrillin: taking a certain amount of fribrillin, and the water mashing of 3 times of weight is added,
50 DEG C and pH 8.0 are adjusted the temperature to, 4000U/g compound protease is added and is hydrolyzed, complex enzyme ratio is Chymetin:
Alkali protease: papain=1:1:0.5, boiling water bath enzyme deactivation 10min terminates reaction after reaction, and flowing water is cooled to room
Temperature is placed in 0 DEG C of refrigerator and saves backup, and gained is grass carp fribrillin enzymolysis product.
(4) grass carp air bladder collagen self assembly: 2.0mg/mL collagen acetum is prepared, in 25 DEG C, pH 6.0
Under the conditions of, 75mmol/mL sodium chloride solution is added, with 1.0mg/mL grass carp fribrillin enzymolysis product, carries out grass carp fish
Ichthyocol albumen self-assembling reaction.
The absorbance value at 313nm is tied up to using spectrophotometer real-time monitoring collagen self-assembly, is drawn from group
Fill curve.Wherein, the collagen self-assembling reaction for being not added with grass carp fribrillin enzymolysis product is control group, addition
The collagen self-assembling reaction of 1.0mg/mL grass carp fribrillin enzymolysis product is experimental group, as a result sees Fig. 2.
As shown in Figure 2, compared with the control group, grass carp air bladder collagen after addition grass carp fribrillin enzymolysis product
Lag phase extend, the absorbance value of the stage of stable development increases, this illustrates that the tridimensional network for the collagenous fibres to be formed is the finest and close,
Addition grass carp fribrillin enzymolysis product can promote the self assembly of grass carp air bladder collagen, show the method for the present embodiment
Obtained grass carp air bladder collagen self assembly effect is preferable.
Embodiment 3
A kind of grass carp air bladder collagen self-assembling method, comprising the following steps:
(1) it is sufficiently washed to neutrality after the degreasing of grass carp air bladder, 0.5M acetic acid (1:10, w/v), 0.1% (w/v) is added
3000U/mg pepsin reacts 48h, and 20min is centrifuged under 5000r/min, and supernatant is added pre-cooling sodium chloride and saltouts, and stands 4h,
Centrifuging and taking precipitates again, and supernatant repeats operation of saltouing, and merges 10 times of volume 0.5M acetate dissolutions of precipitating, dialysis, vacuum refrigeration
- 20 DEG C of preservations are stand-by after drying.
(2) after the grass carp flesh of fish minces, 10 times of volume pre-cooling sodium chloride solutions the extraction of grass carp fribrillin: are added
(70mmol/L) is homogenized, and is placed in ice bath and is stirred 30min, filtered through gauze, is centrifuged 15min under 8000r/min, takes precipitating
The homogenate of pre-cooling sodium chloride solution, centrifugation are added, wash cycles 3 times, gained precipitating is grass carp fribrillin.
(3) enzymatic hydrolysis of grass carp fribrillin: taking a certain amount of fribrillin, and the water mashing of 3 times of weight is added,
50 DEG C and pH 8.0 are adjusted the temperature to, 5000U/g compound protease is added and is hydrolyzed, complex enzyme ratio is Chymetin:
Alkali protease: papain=1:1:0.5, boiling water bath enzyme deactivation 10min terminates reaction after reaction, and flowing water is cooled to room
Temperature is placed in 0 DEG C of refrigerator and saves backup, and gained is grass carp fribrillin enzymolysis product.
(4) grass carp air bladder collagen self assembly: 4.0mg/mL collagen acetum is prepared, in 40 DEG C, pH 7.0
Under, 100mmol/mL sodium chloride solution is added, with 2.0mg/mL grass carp fribrillin enzymolysis product, carries out grass carp fish glue
Former albumen self-assembling reaction.
The absorbance value at 313nm is tied up to using spectrophotometer real-time monitoring collagen self-assembly, is drawn from group
Fill curve.Wherein, the collagen self-assembling reaction for being not added with grass carp fribrillin enzymolysis product is control group, addition
The collagen self-assembling reaction of 2.0mg/mL grass carp fribrillin enzymolysis product is experimental group, as a result sees Fig. 3.
As shown in Figure 3, compared with the control group, grass carp air bladder collagen after addition grass carp fribrillin enzymolysis product
Lag phase extend, the absorbance value of the stage of stable development increases, this illustrates that the tridimensional network for the collagenous fibres to be formed is the finest and close,
Addition grass carp fribrillin enzymolysis product can promote the self assembly of grass carp air bladder collagen, show the method for the present embodiment
Obtained grass carp air bladder collagen self assembly effect is preferable.
Claims (9)
1. a kind of grass carp air bladder collagen self-assembling method, which comprises the following steps:
(1) it is extracted from grass carp and obtains grass carp air bladder collagen；
(2) it is extracted from grass carp and obtains grass carp fribrillin；
(3) the grass carp fribrillin of extraction is digested, obtains grass carp fribrillin enzymolysis product；
(4) grass carp air bladder collagen albumen acetic solution is configured, under conditions of 25-40 DEG C, pH6.0-7.0, it is molten that sodium chloride is added
Liquid carries out grass carp air bladder collagen self-assembling reaction with grass carp fribrillin enzymolysis product.
2. grass carp air bladder collagen self-assembling method as described in claim 1, which is characterized in that the grass carp air bladder collagen
The concentration of albumen acetic solution is 1.0-4.0mg/mL.
3. grass carp air bladder collagen self-assembling method as described in claim 1, which is characterized in that the fribrillin
The concentration of enzymolysis product is 0.5-2.0mg/mL.
4. grass carp air bladder collagen self-assembling method as described in claim 1, which is characterized in that the sodium chloride solution
Concentration is 50-100mmol/mL.
5. grass carp air bladder collagen self-assembling method as described in claim 1, which is characterized in that mentioned in the step (1)
The step of taking grass carp air bladder collagen include:
It will sufficiently be washed to neutrality after grass carp air bladder degreasing, extractant is added and reacts 24-48h, supernatant is added and is pre-chilled by centrifugation
Sodium chloride is saltoutd, and is stood, then centrifuging and taking precipitating, supernatant repeat operation of saltouing, and merge precipitating acetate dissolution, dialysis, vacuum
Preservation is stand-by after freeze-drying.
6. grass carp air bladder collagen self-assembling method as claimed in claim 5, which is characterized in that the extractant includes vinegar
Acid and pepsin.
7. grass carp air bladder collagen self-assembling method as described in claim 1, which is characterized in that mentioned in the step (2)
Take obtain grass carp fribrillin the step of include:
After the grass carp flesh of fish is minced, pre-cooling sodium chloride solution is added and is homogenized, is placed in ice bath and stirs, filter, be centrifuged it
Afterwards, precipitating is taken to add the homogenate of pre-cooling sodium chloride solution, centrifugation, wash cycles, gained precipitating is grass carp fribrillin.
8. grass carp air bladder collagen self-assembling method as described in claim 1, which is characterized in that step (3) medium-height grass
Fish fribrillin digest the step of include:
A certain amount of fribrillin is taken, the water mashing of 3 times of weight is added, adjusts the temperature to 50 DEG C and pH8.0, is added
3000-5000U/g compound protease is hydrolyzed, and complex enzyme ratio is Chymetin: alkali protease: papain=
1:1:0.5, boiling water bath enzyme deactivation 10min terminates reaction after reaction, and flowing water is cooled to room temperature, be placed in 0 DEG C of refrigerator save it is standby
With gained is grass carp fribrillin enzymolysis product.
9. a kind of collagen obtained by any grass carp air bladder collagen self-assembling method of claim 1~8 is raw
Object raw material.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001091821A1 (en) * | 2000-05-26 | 2001-12-06 | Coletica | Collagen-based supports for tissue engineering and preparation of biomaterials |
| JP2007055919A (en) * | 2005-08-23 | 2007-03-08 | Suzuhiro Kamaboko Honten:Kk | Peptide derived from fish meat, method for producing the same, and food containing the peptide |
| CN103172729A (en) * | 2013-03-11 | 2013-06-26 | 北京航空航天大学 | Method for rapidly preparing fish collagen |
| CN107236999A (en) * | 2017-07-17 | 2017-10-10 | 合肥威斯伏新材料有限公司 | A kind of method that fish skin collagen is self-assembled into fiber |
| CN107630059A (en) * | 2016-07-19 | 2018-01-26 | 华南生物医药研究院 | New self assembly collagen and preparation method |
| CN107630060A (en) * | 2016-07-19 | 2018-01-26 | 华南生物医药研究院 | Self assembly collagen and preparation method thereof |
| CN109224128A (en) * | 2017-07-11 | 2019-01-18 | 中国科学院苏州纳米技术与纳米仿生研究所 | Load the slow-releasing system and its construction method of the collagen scaffold of bioactie agent |
-
2019
- 2019-06-25 CN CN201910553200.2A patent/CN110386976B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001091821A1 (en) * | 2000-05-26 | 2001-12-06 | Coletica | Collagen-based supports for tissue engineering and preparation of biomaterials |
| JP2007055919A (en) * | 2005-08-23 | 2007-03-08 | Suzuhiro Kamaboko Honten:Kk | Peptide derived from fish meat, method for producing the same, and food containing the peptide |
| CN103172729A (en) * | 2013-03-11 | 2013-06-26 | 北京航空航天大学 | Method for rapidly preparing fish collagen |
| CN107630059A (en) * | 2016-07-19 | 2018-01-26 | 华南生物医药研究院 | New self assembly collagen and preparation method |
| CN107630060A (en) * | 2016-07-19 | 2018-01-26 | 华南生物医药研究院 | Self assembly collagen and preparation method thereof |
| CN109224128A (en) * | 2017-07-11 | 2019-01-18 | 中国科学院苏州纳米技术与纳米仿生研究所 | Load the slow-releasing system and its construction method of the collagen scaffold of bioactie agent |
| CN107236999A (en) * | 2017-07-17 | 2017-10-10 | 合肥威斯伏新材料有限公司 | A kind of method that fish skin collagen is self-assembled into fiber |
Non-Patent Citations (3)
| Title |
|---|
| SHICHEN ZHU等: "Self-assembly of collagen-based biomaterials: preparation, characterizations and biomedical applications", 《J. MATER. CHEM. B》 * |
| 闫婷婷等: "胶原大分子自组装研究进展", 《高分子通报》 * |
| 陈元昊等: "草鱼皮胶原蛋白的理化性质及其自组装的影响因素分析", 《食品科技》 * |
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