CN110373313A - A kind of automatic culture device of efficiency ethanol strain - Google Patents

A kind of automatic culture device of efficiency ethanol strain Download PDF

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Publication number
CN110373313A
CN110373313A CN201910707709.8A CN201910707709A CN110373313A CN 110373313 A CN110373313 A CN 110373313A CN 201910707709 A CN201910707709 A CN 201910707709A CN 110373313 A CN110373313 A CN 110373313A
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CN
China
Prior art keywords
culture
chamber
sliding
power
cavity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201910707709.8A
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Chinese (zh)
Inventor
程壑
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jinhua Kaisen Intelligent Technology Co Ltd
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Jinhua Kaisen Intelligent Technology Co Ltd
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Application filed by Jinhua Kaisen Intelligent Technology Co Ltd filed Critical Jinhua Kaisen Intelligent Technology Co Ltd
Priority to CN201910707709.8A priority Critical patent/CN110373313A/en
Publication of CN110373313A publication Critical patent/CN110373313A/en
Priority to JP2019207546A priority patent/JP2021023278A/en
Withdrawn legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/12Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kind of automatic culture devices of efficiency ethanol strain, including main body, culture chamber is offered in described, the right side of the culture chamber is opening design, culture mechanism is equipped in the culture chamber, the culture mechanism includes the cunning case being slidably arranged in the culture chamber, opening is offered in the sliding case towards right central cavity, incubator can be stored in the central cavity, ethyl alcohol strain is stored in the incubator, lateral steel pipe is equipped in the roof of the sliding case, the bottom of the transverse direction steel pipe is installed with spray head, the spray head can spray culture solution into the incubator, the present invention passes through the double swerve of sliding case, automatically culture solution and ethyl alcohol strain can be uniformly mixed, eliminate the step of manually mixing, save manpower, improve the degree of automation.

Description

A kind of automatic culture device of efficiency ethanol strain
Technical field
The present invention relates to technical field of cell culture, specially a kind of automatic culture device of efficiency ethanol strain.
Background technique
Alcohol fuel is ideal chose the problems such as solving current energy shortage, environmental pollution, but the life of ethyl alcohol at present Higher cost is produced, the production of ethyl alcohol is usually to carry out fermentation by microorganism to obtain ethyl alcohol, but productivity is lower at present, ethyl alcohol The culture of strain is relatively difficult;
Traditional ethyl alcohol Spawn incubation usually manually adds culture solution and ethyl alcohol strain, then by operator into incubator Member shakes incubator and ethyl alcohol strain manually, is uniformly mixed ethyl alcohol strain with culture solution, is then placed in equipment and is cultivated, And traditional culture device stands firm that space is larger, and the degree of automation is lower, reduces the culture efficiency of ethyl alcohol strain.
Summary of the invention
It is in the prior art for overcoming the purpose of the present invention is to provide a kind of automatic culture device of efficiency ethanol strain Drawbacks described above.
A kind of automatic culture device of efficiency ethanol strain according to the present invention, including main body, it is described in offer culture Chamber, the right side of the culture chamber are opening design, and culture mechanism is equipped in the culture chamber, and the culture mechanism includes that sliding is set The cunning case in the culture chamber is set, opening is offered in the sliding case towards right central cavity, it can be in the central cavity Incubator is stored, ethyl alcohol strain is stored in the incubator, lateral steel pipe, the transverse direction steel pipe are equipped in the roof of the sliding case Bottom be installed with spray head, the spray head can spray culture solution into the incubator, and the bottom of the culture chamber, which offers, to be stirred Chamber is mixed, rabbling mechanism is equipped in the stir chamber, the rabbling mechanism includes rotary stirring rod, and the stirring rod can incite somebody to action Nutrient solution stirs evenly, and the bottom of the stir chamber is connected equipped with pipeline, and sliding slot, institute are offered in the bottom wall of the stir chamber State sliding in sliding slot and be equipped with sliding slot, the stir chamber bottom can close by the sliding block, the stir chamber and the culture chamber it Between offer power cavity, the engine of power can be provided for the culture mechanism and the rabbling mechanism by being equipped in the power cavity Structure.
Further technical solution, rotation is equipped with the agitating shaft extended up and down between the power cavity and the stir chamber, The top of the agitating shaft is installed with the driven wheel of differential in the power cavity, the bottom extent quartile of the agitating shaft In being installed with two groups of stirring rods in the stir chamber, and on its periphery, it is installed with feed inlet in the right wall of the main body, it is described Feed inlet is connected to the stir chamber.
Further technical solution, first motor is embedded in the right wall of the sliding slot admittedly, and the left side of the first motor is dynamic Power, which is connected with screw shaft described in screw shaft and is threadedly engaged with the sliding block, to be connect.
Further technical solution is embedded with the second motor, the left side of second motor in the right wall of the power cavity admittedly Be connected by power worm shaft, fixed dynamic bevel gear on the periphery of the worm shaft, the power bevel gear with it is described driven Bevel gear engages, and offers drive cavity on the left of the power cavity, the drive cavity is connected to the culture chamber, the sliding case Bottom is installed with small slide plate, and the left side extended segment of the power cavity is protruded into the drive cavity, turns on the rear wall of the drive cavity It is dynamic to be equipped with worm gear, it is installed with cam on the leading flank of the worm gear, the cam can be abutted with the small slide plate, the worm gear It is engaged with the worm shaft.
Further technical solution is equipped with two groups of bullets between the left side of the sliding case and the inner left wall of the culture chamber Spring offers inverted draw cut in the roof of the culture chamber, and bend pipe is installed on the top surface of the sliding case, is equipped with rubber in the inverted draw cut Pipe, the pipeline are connected to the rubber tube, and the bend pipe is connected to the rubber tube, and the bend pipe is connected to the spray head, Admittedly water pump is embedded in the pipeline.
Further technical solution, the interior rotation of the roof of the central cavity is equipped with the first horizontal axis that left and right extends, described The right end of first horizontal axis stretches out the right side of the sliding case, and its end is installed with the first block, in the bottom wall of the central cavity Rotation is equipped with the second horizontal axis that left and right extends, and the right end of second horizontal axis is installed with the second block, the right side of first horizontal axis End is installed with belt wheel, and is connected between belt wheel described in two groups by belt.
Further technical solution, the interior sliding of the culture chamber are equipped with baffle, and the baffle is located at the right side of the sliding case, It is installed with fixed plate on the right side of the baffle, hook is hinged on the right side of the main body, the hook can be by institute Fixed plate is stated to catch on.
Further technical solution is installed with handle on the right side of the incubator, by pulling the handle manually.
The beneficial effects of the present invention are: the present invention passes through the double swerve of sliding case, it can be automatically by culture solution and ethyl alcohol bacterium Kind is uniformly mixed, and is eliminated the step of manually mixing, is saved manpower, improve the degree of automation, accelerates ethyl alcohol bacterium Kind culture efficiency various culture solutions are mixed automatically by the rotation of stirring rod, after the completion of mixing, by the work of water pump, Automatically culture solution is injected in incubator, and structure of the invention is simple, easy to operate, stable transmission, occupied space is less.
Detailed description of the invention
Fig. 1 is overall structure diagram inside a kind of automatic culture device of efficiency ethanol strain of the invention;
Fig. 2 is the schematic diagram that mechanism is cultivated in the present invention;
Fig. 3 is the partial enlargement diagram of A in Fig. 1 of the present invention.
Specific embodiment
Below with reference to Fig. 1-3, the present invention is described in detail, for sake of convenience, now such as to hereafter described orientation regulation Under: hereafter described front-rear direction up and down is consistent with the front-rear direction up and down of Fig. 1 projection relation itself.
Referring to Fig.1-3, a kind of automatic culture device of efficiency ethanol strain of embodiment according to the present invention, including main body 10, it is described in offer culture chamber 13, the right side of the culture chamber 13 is opening design, is equipped with culture machine in the culture chamber 13 Structure 71, the culture mechanism 71 includes the cunning case 20 being slidably arranged in the culture chamber 13, is offered out in the sliding case 20 Mouthful towards right central cavity 60, incubator 33 can be stored in the central cavity 60, stores ethyl alcohol bacterium in the incubator 33 Kind, lateral steel pipe 30 is equipped in the roof of the sliding case 20, the bottom of the transverse direction steel pipe 30 is installed with spray head 32, the spray head 32 can spray culture solution into the incubator 33, and the bottom of the culture chamber 13 offers stir chamber 48, the stir chamber 48 Interior to be equipped with rabbling mechanism 72, the rabbling mechanism 72 includes rotary stirring rod 50, and the stirring rod 50 can be by nutrient solution It stirring evenly, the bottom of the stir chamber 48 is connected equipped with pipeline 12, sliding slot 46 is offered in the bottom wall of the stir chamber 48, Sliding is equipped with sliding slot 46 in the sliding slot 46, and 48 bottom of stir chamber can close by the sliding block 45, the stir chamber 48 and Power cavity 40 is offered between the culture chamber 13, being equipped in the power cavity 40 can be the culture mechanism 71 and the stirring The power mechanism 73 of the offer power of mechanism 72.
Valuably or illustratively, rotation is equipped with the stirring extended up and down between the power cavity 40 and the stir chamber 48 Axis 51, the top of the agitating shaft 51 are installed with the driven wheel of differential 41 in the power cavity 40, the agitating shaft 51 Bottom extending portion is located in the stir chamber 48, and two groups of stirring rods 50 are installed on its periphery, the right side of the main body 10 Feed inlet 49 is installed on wall, the feed inlet 49 is connected to the stir chamber 48, is trained by being added into the feed inlet 49 Nutrient solution enters culture solution in the stir chamber 48, then stirs evenly culture solution by the stirring rod 50.
Valuably or illustratively, first motor 47 is embedded in the right wall of the sliding slot 46 admittedly, the first motor 47 Left side power, which is connected with screw shaft 61 described in screw shaft 61 and is threadedly engaged with the sliding block 45, to be connect, and control first electricity is passed through Machine 47 works, and rotates the screw shaft 61, and then the sliding block 45 is allow to horizontally slip, to make the stir chamber 48 Bottom closing is opened.
Valuably or illustratively, the second motor 43, second motor 43 are embedded in the right wall of the power cavity 40 admittedly Left side power be connected with worm shaft 39, fixed dynamic bevel gear 42 on the periphery of the worm shaft 39, the power bores tooth Wheel 42 is engaged with the driven wheel of differential 41, and the left side of the power cavity 40 offers drive cavity 35, the drive cavity 35 and institute The connection of culture chamber 13 is stated, the bottom of the sliding case 20 is installed with small slide plate 36, and institute is protruded into the left side extended segment of the power cavity 40 It states in drive cavity 35, rotation is equipped with worm gear 37 on the rear wall of the drive cavity 35, is installed on the leading flank of the worm gear 37 convex Wheel 38, the cam 38 can be abutted with the small slide plate 36, and the worm gear 37 is engaged with the worm shaft 39, pass through described the The work of two motors 43 rotates the worm shaft 39, and then the rabbling mechanism 72 is made to work, and makes the sliding case 20 can be with It horizontally slips, and then shakes the incubator 33, mix culture solution and ethyl alcohol bacterium.
Valuably or illustratively, two are equipped between the left side of the sliding case 20 and the inner left wall of the culture chamber 13 Spring 14 is organized, inverted draw cut 17 is offered in the roof of the culture chamber 13, is installed with bend pipe 16 on the top surface of the sliding case 20, it is described Rubber tube 15 is equipped in inverted draw cut 17, the pipeline 12 is connected to the rubber tube 15, and the bend pipe 16 connects with the rubber tube 15 Logical, the bend pipe 16 is connected to the spray head 32, admittedly embedded with water pump 11 in the pipeline 12, by the work of the water pump 11, Enter the culture solution in the stir chamber 48 in the bend pipe 16, is then sprayed by the spray head 32 into incubator 33.
Valuably or illustratively, rotation is equipped with the first horizontal axis 28 that left and right extends in the roof of the central cavity 60, The right end of first horizontal axis 28 stretches out the right side of the sliding case 20, and its end is installed with the first block 21, and the middle part is empty Rotation is equipped with the second horizontal axis 24 that left and right extends in the bottom wall of chamber 60, and the right end of second horizontal axis 24 is installed with the second block 23, the right end of first horizontal axis 28 is installed with belt wheel 25, and is connected between belt wheel 25 described in two groups by belt 26, passes through First block 21 is rotated manually, swings first block 21 and second block 23, and then can will be described Incubator 33 limits, and the incubator 33 will not skid off outside the central cavity 60.
Valuably or illustratively, sliding is equipped with baffle 19 in the culture chamber 13, and the baffle 19 is located at the sliding case 20 right side is installed with fixed plate 65 on the right side of the baffle 19, is hinged with hook on the right side of the main body 10 18, the hook 18 can catch on the fixed plate 65, facilitate and take out the incubator 33.
Valuably or illustratively, it is installed with handle 22 on the right side of the incubator 33, it is described by pulling manually Handle 22 facilitates and pulls out the incubator 33.
When in use, ethyl alcohol strain is placed in incubator 33 first, then manual-up slide damper 19, makes to link up with 18 catch on fixed plate 65, and then incubator 33 is pushed into central cavity 60, the first block 21 is can be manually rotated, makes the first block 21 and second block 23 rotate, limited through incubator 33, skid off incubator 33 will not to the right outside central cavity 60, then manually Baffle 19 is put down, the right side of culture chamber 13 is closed, then adds culture solution into feed inlet 49, culture solution is made to enter stirring In chamber 48, the work of the second motor 43 is then controlled, worm shaft 39 is rotated, and then power bevel gear 42 is made to drive driven wheel of differential 41 rotations, make agitating shaft 51 that stirring rod 50 be driven to rotate, culture solution are stirred evenly, and then control first motor 47 and work, make Screw shaft 61 rotates, and then moves to right sliding block 45, opens 48 bottom of stir chamber, then controls water pump 11 and works, makes culture solution Into in lateral steel pipe 30, then spray through spray head 32 into incubator 33, meanwhile, the rotation of worm shaft 39 drives 37 turns of worm gear It is dynamic, make cam 38 that small slide plate 36 be driven to horizontally slip, and then make sliding 20 double swerve of case, mixes ethyl alcohol strain with culture solution It is even, accelerate culture efficiency.
The beneficial effects of the present invention are: the present invention passes through the double swerve of sliding case, it can be automatically by culture solution and ethyl alcohol bacterium Kind is uniformly mixed, and is eliminated the step of manually mixing, is saved manpower, improve the degree of automation, accelerates ethyl alcohol bacterium Kind culture efficiency various culture solutions are mixed automatically by the rotation of stirring rod, after the completion of mixing, by the work of water pump, Automatically culture solution is injected in incubator, and structure of the invention is simple, easy to operate, stable transmission, occupied space is less.
Those skilled in the art can define, can in the case of not departing from overall spirit of the invention and design To make the various modifications for above embodiments.It falls within the scope of protection of the present invention.Protection scheme of the invention It is subject to claims appended hereto.

Claims (8)

1. a kind of automatic culture device of efficiency ethanol strain, including main body, it is characterised in that: culture chamber is offered in described, The right side of the culture chamber is opening design, and culture mechanism is equipped in the culture chamber, and the culture mechanism includes sliding setting Cunning case in the culture chamber offers opening in the sliding case towards right central cavity, can deposit in the central cavity Incubator is put, ethyl alcohol strain is stored in the incubator, lateral steel pipe is equipped in the roof of the sliding case, the transverse direction steel pipe Bottom is installed with spray head, and the spray head can spray culture solution into the incubator;
The bottom of the culture chamber offers stir chamber, rabbling mechanism is equipped in the stir chamber, the rabbling mechanism includes can With the stirring rod of rotation, the stirring rod can stir evenly nutrient solution, and the bottom of the stir chamber is connected equipped with pipeline, institute It states and offers sliding slot in the bottom wall of stir chamber, sliding is equipped with sliding slot in the sliding slot, and the sliding block can be by the stir chamber bottom Closing offers power cavity between the stir chamber and the culture chamber, being equipped in the power cavity can be the culture mechanism The power mechanism of power is provided with the rabbling mechanism.
2. a kind of automatic culture device of efficiency ethanol strain according to claim 1, it is characterised in that: the power cavity with Rotation is equipped with the agitating shaft extended up and down between the stir chamber, and the top of the agitating shaft is installed in the power cavity Driven wheel of differential, the bottom extending portion of the agitating shaft is located in the stir chamber, and is installed with two groups on its periphery and stirs Bar is mixed, feed inlet is installed in the right wall of the main body, the feed inlet is connected to the stir chamber.
3. a kind of automatic culture device of efficiency ethanol strain according to claim 1, it is characterised in that: the right side of the sliding slot Admittedly first motor is embedded in wall, the left side power of the first motor is connected with screw shaft described in screw shaft and the sliding block screw thread It is cooperatively connected.
4. a kind of automatic culture device of efficiency ethanol strain according to claim 2, it is characterised in that: the power cavity Admittedly the second motor is embedded in right wall, the left side power of second motor is connected with worm shaft, consolidates on the periphery of the worm shaft Equipped with power bevel gear, the power bevel gear engages with the driven wheel of differential, and transmission is offered on the left of the power cavity Chamber, the drive cavity are connected to the culture chamber, and the bottom of the sliding case is installed with small slide plate, and the left side of the power cavity extends Section protrudes into the drive cavity, and rotation is equipped with worm gear on the rear wall of the drive cavity, is installed on the leading flank of the worm gear convex Wheel, the cam can be abutted with the small slide plate, and the worm gear is engaged with the worm shaft.
5. a kind of automatic culture device of efficiency ethanol strain according to claim 4, it is characterised in that: a left side for the sliding case Two groups of springs are installed between side and the inner left wall of the culture chamber, offer inverted draw cut in the roof of the culture chamber, it is described It is installed with bend pipe on the top surface of sliding case, is equipped with rubber tube in the inverted draw cut, the pipeline is connected to the rubber tube, the bend pipe It is connected to the rubber tube, the bend pipe is connected to the spray head, is embedded with water pump in the pipeline admittedly.
6. a kind of automatic culture device of efficiency ethanol strain according to claim 5, it is characterised in that: the central cavity Roof in rotation be equipped with the first horizontal axis that left and right extends, the right end of first horizontal axis stretches out the right side of the sliding case, and its End is installed with the first block, and rotation is equipped with the second horizontal axis that left and right extends in the bottom wall of the central cavity, and described second is horizontal The right end of axis is installed with the second block, and the right end of first horizontal axis is installed with belt wheel, and passes through between belt wheel described in two groups Belt connection.
7. a kind of automatic culture device of efficiency ethanol strain according to claim 1, it is characterised in that: in the culture chamber Sliding is equipped with baffle, and the baffle is located at the right side of the sliding case, is installed with fixed plate, the master on the right side of the baffle Hook is hinged on the right side of body, the hook can catch on the fixed plate.
8. a kind of automatic culture device of efficiency ethanol strain according to claim 1, it is characterised in that: the incubator Handle is installed on right side, by pulling the handle manually.
CN201910707709.8A 2019-08-01 2019-08-01 A kind of automatic culture device of efficiency ethanol strain Withdrawn CN110373313A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201910707709.8A CN110373313A (en) 2019-08-01 2019-08-01 A kind of automatic culture device of efficiency ethanol strain
JP2019207546A JP2021023278A (en) 2019-08-01 2019-11-17 High efficiency automatic culture facility for ethanol medium

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Application Number Priority Date Filing Date Title
CN201910707709.8A CN110373313A (en) 2019-08-01 2019-08-01 A kind of automatic culture device of efficiency ethanol strain

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CN114342806A (en) * 2021-12-21 2022-04-15 响水县春秋园农副产品有限公司 Be used for broccoli to plant tender shoot induction culture apparatus
CN115088529B (en) * 2022-07-08 2023-06-16 生态环境部南京环境科学研究所 A simulation culture apparatus for analyzing different crops are to soil cadmium enrichment effect
CN116171889A (en) * 2023-03-31 2023-05-30 徐州生物工程职业技术学院 Duck raising cage with atomization disinfection structure and use method thereof
CN116240088B (en) * 2023-05-09 2023-09-22 翔鹏佑康(北京)科技有限公司 Incubator for oncolytic virus culture and culture method
CN116420585B (en) * 2023-05-11 2023-09-15 西藏自治区高原生物研究所 Ecological environment-friendly orchid culture medium preparation auxiliary device

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CN104611379B (en) * 2015-03-04 2017-12-29 天津大学 A kind of method of microbial fermentation ethanol
JP6739273B2 (en) * 2016-08-02 2020-08-12 株式会社日立製作所 Single-use culture apparatus and culture method
JP6207708B2 (en) * 2016-10-18 2017-10-04 株式会社日立製作所 Automatic culture apparatus and cell culture method
JP6878163B2 (en) * 2017-06-15 2021-05-26 株式会社京都製作所 Culture device and culture method

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Application publication date: 20191025