CN110367240A - A kind of sheep sperm cryo-conservation dilution and method - Google Patents
A kind of sheep sperm cryo-conservation dilution and method Download PDFInfo
- Publication number
- CN110367240A CN110367240A CN201910644961.9A CN201910644961A CN110367240A CN 110367240 A CN110367240 A CN 110367240A CN 201910644961 A CN201910644961 A CN 201910644961A CN 110367240 A CN110367240 A CN 110367240A
- Authority
- CN
- China
- Prior art keywords
- dilution
- sperm
- thousand
- conservation
- penicillin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
Abstract
The invention discloses a kind of sheep sperm cryo-conservation dilution and methods, are related to livestock-raising technical field.The dilution includes following components: glucose 12-29g, sucrose 10-50g, sodium citrate 8-13g, penicillin 60-120 ten thousand IU, streptomysin 60-120 ten thousand IU, distilled water 1000mL.It deposits in bottle after sperm is mixed with dilution by 1:4, is placed in 0-5 DEG C of refrigerator after wrapping up bottle with 4-8 layers of gauze.Dilution effective time-to-live of the present invention is not less than 100 hours；Total time-to-live is not less than 140 hours；Survival index is not less than 69；Acrosomal integrity after saving 48 hours is not less than 72%；Abnormal rate after saving 48 hours is not higher than 18%；Substance used in prepared and diluted liquid of the present invention is chemical reagent entirely, and without animal derived substances such as yolk or milk, securely and reliably, excellent quality is at low cost, and prepared and diluted liquid method is simple.
Description
Technical field
The present invention relates to livestock-raising technical field more particularly to a kind of sheep sperm cryo-conservation dilution and methods.
Background technique
The sperm of sheep cannot survive for a long time in vitro, under sperm motility and fertility are significant after cryo-conservation 24 hours
Drop.To extend the Semen routine time, the sperm adopted should be diluted with good dilution in time.The effect of dilution is
Sperm survival provides new nutrient and energy；Buffer is provided, harm of the pH value variation to sperm in lactic acid forming process is prevented；
Maintain the normal osmotic pressure of sperm and electrolytic equilibrium；Inhibit bacterial growth；It prevents in rapid cooling procedure, low temperature causes cold shock
Injury to sperm；Dilution sperm can greatly increase semen volume, and in order to expand insemination ewe number, this is to the outstanding stud ram of performance
Effect, improvement flock of sheep quality it is highly beneficial.
Be usually used in sheep, the formula basal liquid of goat sperm dilution all answers sterile working to prepare, general production every time is not
It to be more than l weeks dosage.The sperm of sheep such as uses in a few hours after semen collection, general not have to dilution or only diluted more with fresh milk
It is convenient.Fresh milk or goat milk should divide heating disinfection through 92-95 DEG C, 8-15, or after adding 100 milliliters of distilled water dissolutions with 9 grams of milk powder
As dilution, or with 100 milliliters of 3 grams of glucose, 20 milliliters of yolk, 1.4 grams of citric acid and distilled water prepared and diluteds.According to essence
Liquid quality and sperm concentration, diluted multiple is preferably l-3 times.For the activity for inhibiting sperm, reduction metabolism and energy consumption, one
As all using (0-5 DEG C) of low temperature preservation, the time-to-live of sperm under normal temperature condition than significantly extending.But low temperature is cold to what is be skillful in
Stimulation easily causes irreversible shock phenomenon, therefore in addition to adding the Protective substances such as yolk, milk, glycerol in dilution
It outside, should constant temperature as far as possible during preservation it should also be noted that the speed of cooling.
Containing animal derived substances such as lecithalities in traditional dilute liquid, yolk is as object widely applied in semen diluent
Matter has the function of very big.And Recent study is found, using the animal derived raw material containing pathogen can to animal productiong and
Human life causes very big threat.Many countries have put into effect some policies for animal derived raw material at present, or even forbid making
With.There may be pathogenic microorganism in yolk, and this pathogenic microorganism can under the carrying of semen diluent wide-scale distribution, this
The extensive utilization and its exchange internationally of outstanding kind of Genetic Resources of Domestic Animal will be influenced.In addition, not being in yolk
All ingredients are all beneficial to sperm, only lecithin and low-density lipoprotein (LDL) be shield to sperm it is effective
Ingredient.Bulky grain molecular substance possessed by high-density lipoprotein (HDL) in yolk is unfavorable for the survival after sperm freezing, can
The outflow of the substances such as the cholesterol on induced sperm film makes sperm the variation such as capacitation occur.Some substances in yolk can inhibit
The breathing of sperm reduces the survival rate of sperm.Yolk in dilution can also accelerate spermatogenesis capacitation to react, and then make sperm
Energy discharge too early, reduce the time-to-live.In addition the ingredient in yolk due also to selected chicken kind and raise competence
Different and difference, and then influence the quality of dilution.And yolk cannot add in advance, and it can only be at semen dilution scene two
Secondary addition is equivalent to secondary prepared and diluted liquid, troublesome in poeration, time-consuming and laborious, is also not easy to standardize and standardize, is not easy to quotient
Industry metaplasia produces, and the ingredient of non-animal derived property dilution is entirely chemical substance, can be configured in advance, at 0-5 DEG C after disinfection treatment
Under the conditions of can save 1 year as long as and never degenerate, can be directly diluted in semen dilution, it is easy to operate, convenient for commercialization
Production.
Summary of the invention
In view of this, the embodiment of the invention provides a kind of sheep sperm cryo-conservation dilution and method, main purpose
It is to solve the problems, such as that the sheep sperm cryo-conservation time is short, avoid adding animal derived substance, simplify semen dilution operating procedure.
In order to achieve the above objectives, invention broadly provides following technical solutions:
On the one hand, the embodiment of the invention provides a kind of sheep sperm cryo-conservation dilution, the dilution include with
Lower component:
Glucose 12-29g, sucrose 10-50g, sodium citrate 8-13g, penicillin 60-120 ten thousand IU, streptomysin 60-120 ten thousand
IU, distilled water 1000mL.
Preferably, the dilution includes following components: glucose 26-29g, sucrose 10-26g, sodium citrate 10-
13g, penicillin 80-120 ten thousand IU, streptomysin 80-120 ten thousand IU, distilled water 1000mL.
Preferably, the dilution includes following components: glucose 20-26g, sucrose 16-45g, sodium citrate 9-
13g, penicillin 100-120 ten thousand IU, streptomysin 100-120 ten thousand IU, distilled water 1000mL.
Preferably, the dilution includes following components: glucose 12-20g, sucrose 30-50g, sodium citrate 8-
13g, penicillin 110-120 ten thousand IU, streptomysin 110-120 ten thousand IU, distilled water 1000mL.
On the other hand, the embodiment of the invention provides a kind of sheep sperm Cryopreservation, the method includes following
Step:
The sperm of passed examination is divided in the penicillin bottle of sterilization treatment, it is then that the penicillin equipped with sperm is small
Bottle is placed in simultaneously in 30-35 DEG C of thermostat water bath with dilution, 30 minutes isothermal processes is carried out, then by dilution edge
The wall of penicillin bottle is slowly added to, and is gently shaken, and is allowed to uniformly mixed, and the volume ratio of sperm and dilution is 1:4, then
It is placed in after wrapping up the penicillin bottle with 4-8 layers of gauze in the refrigerator that temperature is 0-5 DEG C；Wherein, the dilution is power
Benefit requires a kind of sheep sperm cryo-conservation dilution described in any of the above embodiments.
Compared with prior art, the beneficial effects of the present invention are:
It is cumbersome when the present invention is short there are the time for sheep sperm cryo-conservation dilution, survival rate is low and dilution
Technical problem devises a group of formula, which avoids using animal derived substance (such as under the premise of guaranteeing to save quality
Yolk or milk in traditional dilute agent), traditional sheep sperm cryo-conservation technology is become one by prepared and diluted liquid twice
Secondary prepared and diluted liquid, simplifies operating procedure, and preparation method is simple, and at low cost, using effect is reliable, low convenient for sheep sperm
Temperature saves the standardization and standardization of dilution, and commercially producing and selling convenient for sheep sperm cryo-conservation dilution is
The large-scale promotion application of sheep technology of artificial insemination provides technical support.
Specific embodiment
For further illustrate the present invention to reach the technical means and efficacy that predetermined goal of the invention is taken, below with compared with
Good embodiment, to specific embodiment, technical solution, feature and its effect applied according to the present invention, detailed description is as follows.Under
Stating the special characteristic, structure or feature in multiple embodiments in bright can be combined by any suitable form.
Embodiment
A kind of sheep sperm cryo-conservation dilution, including following components: glucose 12-29g, sucrose 10-50g, lemon
Sour sodium 8-13g, penicillin 60-120 ten thousand IU, streptomysin 60-120 ten thousand IU, distilled water 1000mL；
It is deposited in bottle after sperm is mixed with dilution according to volume ratio for 1:4, it is described small with 4-8 layers of gauzes package
It is placed in after bottle in the refrigerator that temperature is 0-4 DEG C.
As the preferred of above-described embodiment, the dilution includes following components: glucose 26-29g, sucrose 10-26g,
Sodium citrate 10-13g, penicillin 80-120 ten thousand IU, streptomysin 80-120 ten thousand IU, distilled water 1000mL.
As the preferred of above-described embodiment, the dilution includes following components: glucose 20-26g, sucrose 16-45g,
Sodium citrate 9-13g, penicillin 100-120 ten thousand IU, streptomysin 100-120 ten thousand IU, distilled water 1000mL.
As the preferred of above-described embodiment, the dilution includes following components: glucose 12-20g, sucrose 30-50g,
Sodium citrate 8-13g, penicillin 110-120 ten thousand IU, streptomysin 110-120 ten thousand IU, distilled water 1000mL.
Diluent of the invention can reach standard requirements: dilution effective time-to-live of the present invention is not less than 100 hours；Always
Time-to-live is not less than 140 hours；Survival index is not less than 69；Acrosomal integrity after saving 48 hours is not less than 72%；It protects
Abnormal rate after depositing 48 hours is not higher than 18%.
Place, those skilled in the art can not select from the prior art to the greatest extent in the embodiment of the present invention.
Disclosed above is only a specific embodiment of the invention, but scope of protection of the present invention is not limited thereto, is appointed
What those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, answer
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be with above-mentioned scope of protection of the claims
It is quasi-.
Claims (5)
1. a kind of sheep sperm cryo-conservation dilution, which is characterized in that the dilution includes following components:
Glucose 12-29g, sucrose 10-50g, sodium citrate 8-13g, penicillin 60-120 ten thousand IU of ten thousand IU, streptomysin 60-120,
Distilled water 1000mL.
2. a kind of sheep sperm cryo-conservation dilution as described in claim 1, which is characterized in that the dilution include with
Lower component: glucose 26-29g, sucrose 10-26g, sodium citrate 10-13g, penicillin 80-120 ten thousand IU, streptomysin 80-120 ten thousand
IU, distilled water 1000mL.
3. a kind of sheep sperm cryo-conservation dilution as described in claim 1, which is characterized in that the dilution include with
Lower component: glucose 20-26g, sucrose 16-45g, sodium citrate 9-13g, penicillin 100-120 ten thousand IU, streptomysin 100-120
Ten thousand IU, distilled water 1000mL.
4. a kind of sheep sperm cryo-conservation dilution as described in claim 1, which is characterized in that the dilution include with
Lower component: glucose 12-20g, sucrose 30-50g, sodium citrate 8-13g, penicillin 110-120 ten thousand IU, streptomysin 110-120
Ten thousand IU, distilled water 1000mL.
5. a kind of sheep sperm Cryopreservation, which is characterized in that the described method comprises the following steps:
The sperm of passed examination is divided in the penicillin bottle of sterilization treatment, then by equipped with sperm penicillin bottle with
Dilution is placed in 30-35 DEG C of thermostat water bath simultaneously, 30 minutes isothermal processes is carried out, then by dilution along mould
The wall of plain bottle is slowly added to, and is gently shaken, and is allowed to uniformly mixed, and the volume ratio of sperm and dilution is 1:4, then uses 4-
8 layers of gauze are placed in the refrigerator that temperature is 0-5 DEG C after wrapping up the penicillin bottle；Wherein, the dilution is wanted for right
Seek a kind of described in any item sheep sperm cryo-conservation dilutions of 1-4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910644961.9A CN110367240A (en) | 2019-07-17 | 2019-07-17 | A kind of sheep sperm cryo-conservation dilution and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910644961.9A CN110367240A (en) | 2019-07-17 | 2019-07-17 | A kind of sheep sperm cryo-conservation dilution and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110367240A true CN110367240A (en) | 2019-10-25 |
Family
ID=68253620
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910644961.9A Pending CN110367240A (en) | 2019-07-17 | 2019-07-17 | A kind of sheep sperm cryo-conservation dilution and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110367240A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113615682A (en) * | 2021-09-05 | 2021-11-09 | 内蒙古赛诺种羊科技有限公司 | Sheep hypertonic complexing agent semen dilution preserving fluid and application method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010005260A2 (en) * | 2008-07-10 | 2010-01-14 | 주식회사 노아바이오텍 | Diluent for long-term preservation of liquid boar sperm |
| CN102986647A (en) * | 2011-09-08 | 2013-03-27 | 辽宁省辽宁绒山羊原种场有限公司 | Non-animal-origin frozen semen diluent |
| CN106857500A (en) * | 2017-02-06 | 2017-06-20 | 张志刚 | The store method and its special fresh essence of a kind of fresh essence of animal preserve dilution |
| CN107467010A (en) * | 2017-08-11 | 2017-12-15 | 田东雄桂牧业有限公司 | A kind of boar semen releases thin liquid |
-
2019
- 2019-07-17 CN CN201910644961.9A patent/CN110367240A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010005260A2 (en) * | 2008-07-10 | 2010-01-14 | 주식회사 노아바이오텍 | Diluent for long-term preservation of liquid boar sperm |
| CN102986647A (en) * | 2011-09-08 | 2013-03-27 | 辽宁省辽宁绒山羊原种场有限公司 | Non-animal-origin frozen semen diluent |
| CN106857500A (en) * | 2017-02-06 | 2017-06-20 | 张志刚 | The store method and its special fresh essence of a kind of fresh essence of animal preserve dilution |
| CN107467010A (en) * | 2017-08-11 | 2017-12-15 | 田东雄桂牧业有限公司 | A kind of boar semen releases thin liquid |
Non-Patent Citations (1)
| Title |
|---|
| 权凯 等: "《肉羊良种利用与繁殖技术》", 30 June 2018 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113615682A (en) * | 2021-09-05 | 2021-11-09 | 内蒙古赛诺种羊科技有限公司 | Sheep hypertonic complexing agent semen dilution preserving fluid and application method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8460926B2 (en) | Aqueous solution for cell preservation | |
| CN108434954B (en) | It is a kind of multi-functional except formaldehyde deodorization eliminating smell agent | |
| CN101180981B (en) | Fresh-keeping method for low temperature birds meat products | |
| CN107912419A (en) | The frozen stock solution and cryopreservation methods of a kind of human peripheral blood single nucleus cell | |
| CN110367240A (en) | A kind of sheep sperm cryo-conservation dilution and method | |
| CN102986647B (en) | Non-animal-origin frozen semen diluent | |
| CN109042874A (en) | A kind of preparation method of sterilization cream | |
| KR101457526B1 (en) | Diluent Compound for Improving Pig Sperm Preservation Ability | |
| CN103704338B (en) | Production method for ultrahigh-pressure homogenization and sterilization milk | |
| CN110301431A (en) | A kind of non-animal derived property sheep sperm cryo-conservation dilution and method | |
| CN106359531A (en) | Pork freezing process | |
| CN107455443A (en) | A kind of quick-frozen liquid of food fresh keeping and preparation method thereof and application method | |
| CN106818709A (en) | Cattle freezing seminal fluid dilution preparation method | |
| CN106332950A (en) | Frozen pork fresh-keeping technology | |
| CN105495483A (en) | Method for making bacteriostatic-agent dry powder with pickle brine | |
| CN105941391B (en) | A kind of hybridization snakehead spermatozoa preservative fluid and its application | |
| CN110419681A (en) | A kind of production method and its application of low-temperature meat product | |
| CN109757551A (en) | A kind of tea polyphenol liposomes/lysozyme-chitosan sustained-release film preparation method fresh-keeping applied to fillet | |
| CN105075849A (en) | Hand-pollinate method for kiwifruit tree planting | |
| CN102058121B (en) | Method for processing freshly squeezed mulberry juice | |
| CN101411350A (en) | Meat product improver | |
| CN110583630A (en) | Donkey semen diluent and preparation method thereof | |
| CN102067902A (en) | Meat product antistaling agent | |
| CN107177044A (en) | A kind of collagen-Tea Polyphenols antibacterial film and preparation method thereof | |
| CN101999342A (en) | Novel preparation method of yolk diluent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |