CN110301431A - A kind of non-animal derived property sheep sperm cryo-conservation dilution and method - Google Patents
A kind of non-animal derived property sheep sperm cryo-conservation dilution and method Download PDFInfo
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- CN110301431A CN110301431A CN201910648002.4A CN201910648002A CN110301431A CN 110301431 A CN110301431 A CN 110301431A CN 201910648002 A CN201910648002 A CN 201910648002A CN 110301431 A CN110301431 A CN 110301431A
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- 238000010790 dilution Methods 0.000 title claims abstract description 48
- 239000012895 dilution Substances 0.000 title claims abstract description 48
- 241001494479 Pecora Species 0.000 title claims abstract description 25
- 241001465754 Metazoa Species 0.000 title claims abstract description 22
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 13
- 229930182555 Penicillin Natural products 0.000 claims abstract description 20
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims abstract description 20
- 229940049954 penicillin Drugs 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000012153 distilled water Substances 0.000 claims abstract description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 14
- 239000008103 glucose Substances 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 14
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 13
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 13
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 13
- 239000001509 sodium citrate Substances 0.000 claims abstract description 13
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 210000002969 egg yolk Anatomy 0.000 abstract description 12
- 239000000126 substance Substances 0.000 abstract description 10
- 235000013336 milk Nutrition 0.000 abstract description 6
- 239000008267 milk Substances 0.000 abstract description 6
- 210000004080 milk Anatomy 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 6
- 230000002159 abnormal effect Effects 0.000 abstract description 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 210000000582 semen Anatomy 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 102000015779 HDL Lipoproteins Human genes 0.000 description 2
- 108010010234 HDL Lipoproteins Proteins 0.000 description 2
- 102000007330 LDL Lipoproteins Human genes 0.000 description 2
- 108010007622 LDL Lipoproteins Proteins 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 230000008010 sperm capacitation Effects 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 235000020251 goat milk Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000021595 spermatogenesis Effects 0.000 description 1
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- -1 yolk Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of non-animal derived property sheep sperm cryo-conservation dilution and methods, are related to livestock-raising technical field.The dilution includes following components: glucose 12-29g, trehalose 10-50g, sodium citrate 8-13g, penicillin 60-120 ten thousand IU, streptomysin 60-120 ten thousand IU, distilled water 1000mL.It deposits in bottle after sperm is mixed under 30-35 DEG C of isothermy with dilution by 1:4, is placed in 0-5 DEG C of refrigerator after wrapping up bottle with 4-8 layers of gauze.Dilution effective time-to-live of the present invention is not less than 130h；Total time-to-live is not less than 180h；Survival index is not less than 90；Acrosomal integrity after saving 48h is not less than 75%；Abnormal rate after saving 48h is not higher than 13%；Substance used in prepared and diluted liquid of the present invention is chemical reagent entirely, and without animal derived substances such as yolk or milk, securely and reliably, excellent quality is at low cost, and prepared and diluted liquid method is simple.
Description
Technical field
The present invention relates to livestock-raising technical field more particularly to a kind of non-animal derived property sheep sperm cryo-conservation dilutions
Liquid and method.
Background technique
The sperm of sheep cannot survive for a long time in vitro, under sperm motility and fertility are significant after cryo-conservation 24 hours
Drop.To extend the Semen routine time, the sperm adopted should be diluted with good dilution in time.The effect of dilution is
Sperm survival provides new nutrient and energy；Buffer is provided, harm of the pH value variation to sperm in lactic acid forming process is prevented；
Maintain the normal osmotic pressure of sperm and electrolytic equilibrium；Inhibit bacterial growth；It prevents in rapid cooling procedure, low temperature causes cold shock
Injury to sperm；Dilution sperm can greatly increase semen volume, and in order to expand insemination ewe number, this is to giving full play to outstanding kind
The effect of ram, improvement flock of sheep quality are highly beneficial.
Be usually used in sheep, the formula basal liquid of goat sperm dilution all answers sterile working to prepare, general production every time is not
It to be more than l weeks dosage.The sperm of sheep such as uses in a few hours after semen collection, general not have to dilution or only diluted more with fresh milk
It is convenient.Fresh milk or goat milk should divide heating disinfection through 92-95 DEG C, 8-15, or after adding 100 milliliters of distilled water dissolutions with 9 grams of milk powder
As dilution, or with 100 milliliters of 3 grams of glucose, 20 milliliters of yolk, 1.4 grams of citric acid and distilled water prepared and diluteds.According to essence
Liquid quality and sperm concentration, diluted multiple is preferably l-3 times.For the activity for inhibiting sperm, reduction metabolism and energy consumption, one
As all using (0-5 DEG C) of low temperature preservation, the time-to-live of sperm under normal temperature condition than significantly extending.But low temperature is cold to what is be skillful in
Stimulation easily causes irreversible shock phenomenon, therefore in addition to adding the Protective substances such as yolk, milk, glycerol in dilution
It outside, should constant temperature as far as possible during preservation it should also be noted that the speed of cooling.
Containing animal derived substances such as lecithalities in traditional dilute liquid, yolk is as object widely applied in semen diluent
Matter has the function of very big.And Recent study is found, using the animal derived raw material containing pathogen can to animal productiong and
Human life causes very big threat.Many countries have put into effect some policies for animal derived raw material at present, or even forbid making
With.There may be pathogenic microorganism in yolk, and this pathogenic microorganism can under the carrying of semen diluent wide-scale distribution, this
The extensive utilization and its exchange internationally of outstanding kind of Genetic Resources of Domestic Animal will be influenced.In addition, not being in yolk
All ingredients are all beneficial to sperm, only lecithin and low-density lipoprotein (LDL) be shield to sperm it is effective
Ingredient.Bulky grain molecular substance possessed by high-density lipoprotein (HDL) in yolk is unfavorable for the survival after sperm freezing, can
The outflow of the substances such as the cholesterol on induced sperm film makes sperm the variation such as capacitation occur.Some substances in yolk can inhibit
The breathing of sperm reduces the survival rate of sperm.Yolk in dilution can also accelerate spermatogenesis capacitation to react, and then make sperm
Energy discharge too early, reduce the time-to-live.In addition the ingredient in yolk due also to selected chicken kind and raise competence
Different and difference, and then influence the quality of dilution.And yolk cannot add in advance, and it can only be at semen dilution scene two
Secondary addition is equivalent to secondary prepared and diluted liquid, troublesome in poeration, time-consuming and laborious, is also not easy to standardize and standardize, is not easy to quotient
Industry metaplasia produces, and the ingredient of non-animal derived property dilution is entirely chemical substance, can be configured in advance, at 0-5 DEG C after disinfection treatment
Under the conditions of can save 1 year as long as and never degenerate, can be directly diluted in semen dilution, it is easy to operate, convenient for commercialization
Production.
Summary of the invention
In view of this, the embodiment of the invention provides a kind of non-animal derived property sheep sperm cryo-conservation dilution and sides
Method, main purpose are that the solution sheep sperm cryo-conservation time is short, avoid adding animal derived substance, simplify semen dilution operation
The problem of step.
In order to achieve the above objectives, invention broadly provides following technical solutions:
On the one hand, described dilute the embodiment of the invention provides a kind of non-animal derived property sheep sperm cryo-conservation dilution
Releasing liquid includes following components:
Glucose 12-29g, trehalose 10-50g, sodium citrate 8-13g, penicillin 60-120 ten thousand IU, streptomysin 60-120
Ten thousand IU, distilled water 1000mL.
Preferably, the dilution includes following components: glucose 26-29g, trehalose 10-26g, sodium citrate 10-
13g, penicillin 80-120 ten thousand IU, streptomysin 80-120 ten thousand IU, distilled water 1000mL.
Preferably, the dilution includes following components: glucose 20-26g, trehalose 16-45g, sodium citrate 9-
13g, penicillin 100-120 ten thousand IU, streptomysin 100-120 ten thousand IU, distilled water 1000mL.
Preferably, the dilution includes following components: glucose 12-20g, trehalose 30-50g, sodium citrate 8-
13g, penicillin 110-120 ten thousand IU, streptomysin 110-120 ten thousand IU, distilled water 1000mL.
On the other hand, the embodiment of the invention provides a kind of non-animal derived property sheep sperm Cryopreservation, the sides
Method, then will be equipped with sperm the following steps are included: the sperm of passed examination is divided in the penicillin bottle of sterilization treatment
Penicillin bottle and dilution are placed in simultaneously in 30-35 DEG C of thermostat water bath, carry out 30 minutes isothermal processes, then will
Dilution is slowly added to along the wall of penicillin bottle, and is gently shaken, and is allowed to uniformly mixed, and the volume ratio of sperm and dilution is
1:4 is placed in the refrigerator that temperature is 0-5 DEG C after then wrapping up the penicillin bottle with 4-8 layers of gauze；Wherein, described dilute
Releasing liquid is a kind of above-mentioned sheep sperm cryo-conservation dilution.
Compared with prior art, the beneficial effects of the present invention are:
It is cumbersome when the present invention is short there are the time for sheep sperm cryo-conservation dilution, survival rate is low and dilution
Technical problem devises a group of formula, which avoids using animal derived substance (such as under the premise of guaranteeing to save quality
Yolk or milk in traditional dilute agent), traditional sheep sperm cryo-conservation technology is become one by prepared and diluted liquid twice
Secondary prepared and diluted liquid, simplifies operating procedure, and preparation method is simple, and at low cost, using effect is reliable, low convenient for sheep sperm
Temperature saves the standardization and standardization of dilution, and commercially producing and selling convenient for sheep sperm cryo-conservation dilution is
The large-scale promotion application of sheep technology of artificial insemination provides technical support and material guarantee.
Specific embodiment
For further illustrate the present invention to reach the technical means and efficacy that predetermined goal of the invention is taken, below with compared with
Good embodiment, to specific embodiment, technical solution, feature and its effect applied according to the present invention, detailed description is as follows.Under
Stating the special characteristic, structure or feature in multiple embodiments in bright can be combined by any suitable form.
Embodiment
A kind of non-animal derived property sheep sperm cryo-conservation dilution, including following components: glucose 12-29g, trehalose
10-50g, sodium citrate 8-13g, penicillin 60-120 ten thousand IU, streptomysin 60-120 ten thousand IU, distilled water 1000mL；
It is deposited in bottle after sperm is mixed under 30-35 DEG C of isothermy according to volume ratio for 1:4 with dilution,
It is placed in after wrapping up the bottle with 4-8 layers of gauze in the refrigerator that temperature is 0-5 DEG C.
As the preferred of above-described embodiment, the dilution includes following components: glucose 26-29g, trehalose 10-
26g, sodium citrate 10-13g, penicillin 80-120 ten thousand IU, streptomysin 80-120 ten thousand IU, distilled water 1000mL.
As the preferred of above-described embodiment, the dilution includes following components: glucose 20-26g, trehalose 16-
45g, sodium citrate 9-13g, penicillin 100-120 ten thousand IU, streptomysin 100-120 ten thousand IU, distilled water 1000mL.
As the preferred of above-described embodiment, the dilution includes following components: glucose 12-20g, trehalose 30-
50g, sodium citrate 8-13g, penicillin 110-120 ten thousand IU, streptomysin 110-120 ten thousand IU, distilled water 1000mL.
Diluent of the invention can reach standard requirements: effective time-to-live is not less than 130 hours；Total time-to-live is not low
In 180 hours；Survival index is not less than 90；Replacement percentage of head rice after saving 48 hours is not less than 75%；After saving 48 hours
Abnormal rate is not higher than 13%.
Place, those skilled in the art can not select from the prior art to the greatest extent in the embodiment of the present invention.
Disclosed above is only a specific embodiment of the invention, but scope of protection of the present invention is not limited thereto, is appointed
What those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, answer
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be with above-mentioned scope of protection of the claims
It is quasi-.
Claims (5)
1. a kind of non-animal derived property sheep sperm cryo-conservation dilution, which is characterized in that the dilution includes following components:
Glucose 12-29g, trehalose 10-50g, sodium citrate 8-13g, penicillin 60-120 ten thousand IU, streptomysin 60-120 ten thousand
IU, distilled water 1000mL.
2. a kind of non-animal derived property sheep sperm cryo-conservation dilution as described in claim 1, which is characterized in that described dilute
Releasing liquid includes following components: glucose 26-29g, trehalose 10-26g, sodium citrate 10-13g, ten thousand IU of penicillin 80-120, chain
Mycin 80-120 ten thousand IU, distilled water 1000mL.
3. a kind of non-animal derived property sheep sperm cryo-conservation dilution as described in claim 1, which is characterized in that described dilute
Releasing liquid includes following components: glucose 20-26g, trehalose 16-45g, sodium citrate 9-13g, ten thousand IU of penicillin 100-120, chain
Mycin 100-120 ten thousand IU, distilled water 1000mL.
4. a kind of non-animal derived property sheep sperm cryo-conservation dilution as described in claim 1, which is characterized in that described dilute
Releasing liquid includes following components: glucose 12-20g, trehalose 30-50g, sodium citrate 8-13g, ten thousand IU of penicillin 110-120, chain
Mycin 110-120 ten thousand IU, distilled water 1000mL.
5. a kind of non-animal derived property sheep sperm Cryopreservation, which is characterized in that the described method comprises the following steps:
The sperm of passed examination is divided in the penicillin bottle of sterilization treatment, then by equipped with sperm penicillin bottle with
Dilution is placed in 30-35 DEG C of thermostat water bath simultaneously, 30 minutes isothermal processes is carried out, then by dilution along mould
The wall of plain bottle is slowly added to, and is gently shaken, and is allowed to uniformly mixed, and the volume ratio of sperm and dilution is 1:4, then uses 4-
8 layers of gauze are placed in the refrigerator that temperature is 0-5 DEG C after wrapping up the penicillin bottle；Wherein, the dilution is wanted for right
Seek a kind of described in any item sheep sperm cryo-conservation dilutions of 1-4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910648002.4A CN110301431A (en) | 2019-07-17 | 2019-07-17 | A kind of non-animal derived property sheep sperm cryo-conservation dilution and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910648002.4A CN110301431A (en) | 2019-07-17 | 2019-07-17 | A kind of non-animal derived property sheep sperm cryo-conservation dilution and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110301431A true CN110301431A (en) | 2019-10-08 |
Family
ID=68080564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910648002.4A Pending CN110301431A (en) | 2019-07-17 | 2019-07-17 | A kind of non-animal derived property sheep sperm cryo-conservation dilution and method |
Country Status (1)
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010005260A2 (en) * | 2008-07-10 | 2010-01-14 | 주식회사 노아바이오텍 | Diluent for long-term preservation of liquid boar sperm |
| CN102986647A (en) * | 2011-09-08 | 2013-03-27 | 辽宁省辽宁绒山羊原种场有限公司 | Non-animal-origin frozen semen diluent |
| CN106857500A (en) * | 2017-02-06 | 2017-06-20 | 张志刚 | The store method and its special fresh essence of a kind of fresh essence of animal preserve dilution |
| CN107467010A (en) * | 2017-08-11 | 2017-12-15 | 田东雄桂牧业有限公司 | A kind of boar semen releases thin liquid |
-
2019
- 2019-07-17 CN CN201910648002.4A patent/CN110301431A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010005260A2 (en) * | 2008-07-10 | 2010-01-14 | 주식회사 노아바이오텍 | Diluent for long-term preservation of liquid boar sperm |
| CN102986647A (en) * | 2011-09-08 | 2013-03-27 | 辽宁省辽宁绒山羊原种场有限公司 | Non-animal-origin frozen semen diluent |
| CN106857500A (en) * | 2017-02-06 | 2017-06-20 | 张志刚 | The store method and its special fresh essence of a kind of fresh essence of animal preserve dilution |
| CN107467010A (en) * | 2017-08-11 | 2017-12-15 | 田东雄桂牧业有限公司 | A kind of boar semen releases thin liquid |
Non-Patent Citations (1)
| Title |
|---|
| 权凯 等: "《肉羊良种利用与繁殖技术》", 30 June 2018 * |
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Application publication date: 20191008 |
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