CN110361382A - A kind of chromosome scanning imaging system - Google Patents
A kind of chromosome scanning imaging system Download PDFInfo
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- CN110361382A CN110361382A CN201811633191.XA CN201811633191A CN110361382A CN 110361382 A CN110361382 A CN 110361382A CN 201811633191 A CN201811633191 A CN 201811633191A CN 110361382 A CN110361382 A CN 110361382A
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- detectable substance
- slide
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- control system
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- 210000000349 chromosome Anatomy 0.000 title claims abstract description 43
- 238000003384 imaging method Methods 0.000 title claims abstract description 29
- 239000000126 substance Substances 0.000 claims abstract description 74
- 230000003287 optical effect Effects 0.000 claims description 7
- 208000011117 substance-related disease Diseases 0.000 claims description 2
- 238000012163 sequencing technique Methods 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 239000003921 oil Substances 0.000 description 13
- 238000005516 engineering process Methods 0.000 description 10
- 239000011521 glass Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000010627 cedar oil Substances 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000003243 Thuja occidentalis Species 0.000 description 1
- 235000008109 Thuja occidentalis Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- -1 slide Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
Abstract
A kind of chromosome scanning imaging system, at least it is made of slide film feeding apparatus, microscopic system and overall control system, while wherein sequentially observing detectable substance using overall control system control camera unit with the object lens with minimum multiple to the object lens with highest multiple, with three-wheel continuation mode Scanning Detction object, the first wheel scan is to obtain the region that detectable substance is distributed；Second wheel scan carries out full sheet scanning to obtain the focal plane under second multiple to obtain multiple images under the second multiple, and the detectable substance of the image by overall control system for obtaining under the second multiple is ranked up and positions；And third wheel scan, camera unit focus shooting according to positioning for the forward detectable substance that sorts using the object lens of highest multiple and obtain a series of high-definition images to provide the diagnosis or treatment of disease by high-definition image.
Description
Technical field
The present invention is to be related to a kind of chromosome technique field, is swept in particular to chromosome scanning imaging system and imaging
Retouch method.
Background technique
Many medical diagnosis test need doctor by using utensil swipe and/or scraping crust or mucous membrane in target area and
Collect cell.The cell of collection is usually smeared into (or fixed) on glass slide, and is dyed to facilitate cell
The inspection of technology scholar and/or pathologist under the microscope.For example, virologist can be used via staining cell core portion
Part and the polychrome technology that characterizes determines presence that depauperation or tumor are formed.Virologist also can Comparison study dyeing so as to
Inspect the cytoplasm of cell.
The mechanical structure of current scanline imaging system is by the device of storage glass slide, mechanical arm, microscope and micro-
Mirror article carrying platform composition.Manipulator takes piece in the device of storage glass slide through process control, and it is flat to be placed on microscope slide
On platform, via the chromosome or cell on micro- scarnning mirror, film making and analysis glass slide.However to capture the dyeing of glass slide
The high definition pattern of body or cell, typically after photographing discovery pattern do not know or lack of resolution, can just replace
The object lens of different multiples are scanned and are shot, but very expend time and manpower.Also, when the substantial amounts of detectable substance,
Current micro- scanning system once can only be scanned and capture pattern to a glass slide, and use the object of the same multiple
Mirror also can not necessarily capture to the clear image of resolution ratio all detectable substances, require to lean on artificial judgement replacement difference
It rescans and shoots after the object lens of multiple, do not meet very much economic benefit.
Summary of the invention
The main purpose of the present invention is to provide a kind of chromosome scanning imaging systems, are controlled using overall control system
Multiple storings have the slide of detectable substance to see in such a way that three-wheel is continuous to the detectable substance in microscope way moving objective table
It surveys and utilizes scanning element under the object lens of three different multiples, the detection on each slide is sequentially scanned by low power to high power
Object, and multiple photographs are shot under each multiple.
It is a further object of the present invention to provide a kind of chromosome scanning imaging system, using in overall control system come for
The detectable substance for multiple images that each detectable substance is obtained as captured by camera unit is ranked up, and leans on sequence via after sequence
Preceding detectable substance, which focuses to shoot according to positioning, obtains a series of high-definition images to provide subsequent analysis or diagnosis and use.
According to above-mentioned purpose, the present invention discloses a kind of chromosome scanning imaging system, at least by slide film feeding apparatus, micro-
Mirror system and overall control system are formed, and wherein slide film feeding apparatus includes multiple slide boxs, are had in each slide box
There is detectable substance on multiple slides and each slide；Microscopic system includes: microscope way moving objective table, camera shooting are single
Member, optical path unit and object lens, wherein object lens are made of sequentially the object lens of three different multiples by minimum multiple
Detectable substance is observed to highest multiple；And overall control system has even for system, it is even mechanical by X-Y-Z three-shaft linkage for system
Arm forms, and the Y-axis in X-Y-Z triaxial linkage mechanical hand arm is basic axis, so that even being moved for system with front and rear direction, Z axis
Left and right direction movement is carried out on Z axis with upper and lower direction movement and X-axis in Y-axis, even for system by overall control system
Control picks and places slide from slide film feeding apparatus on microscope way moving objective table.
The present invention is main to be technically characterized in that overall control system further includes；Control microscope way moving objective table
Movement, so that microscopic system controls microscope way moving objective table in a first direction and second direction moves, and by aobvious
Micromirror systems control object lens and control microscope way moving objective table as object lens are in third direction in the movement of third direction
Movement；Object lens are controlled by the object lens with minimum multiple to the object lens with highest multiple sequentially in the two-way shifting of microscope
The detectable substance on slide in dynamic object stage is observed；Overall control system control microscopic system in camera unit to
The detectable substance on slide on microscope way moving objective table is scanned, and is judged according to the object lens with minimum multiple
Whether detectable substance uses three wheel scans, if so, overall control system control microscopic system in camera unit sequentially with
With three-wheel continuation mode Scanning Detction while object lens with minimum multiple to the object lens with highest multiple observe detectable substance
Object, wherein overall control system control camera unit executes the first wheel scan, and camera unit is for the object lens with minimum multiple
Entire scan is carried out to obtain the region of detectable substance distribution for the detectable substance on slide；Overall control system control camera shooting is single
Member executes the second wheel scan, and camera unit utilizes the point in the region that the object lens of the second multiple are observed and detectable substance is selected to be distributed
And it is focused to obtain the focal plane under second multiple, and carries out full sheet scanning to obtain under the second multiple
Multiple images, and the image obtained under the second multiple is ranked up and is positioned by overall control system；And overall control
System control camera unit processed executes third wheel scan, and camera unit focuses shooting using the object lens positioning of highest multiple and obtains one
Serial high-definition image, to provide the diagnosis or treatment of disease by high-definition image.
Detailed description of the invention
Fig. 1 and Fig. 2 is technology disclosed according to the present invention, indicates the front view of chromosome scanning imagery board.
Fig. 3 is technology disclosed according to the present invention, according to chromosome scanning imagery board represented by Fig. 1 and Fig. 2 into
One step is with chromosome scanning imaging system come the block schematic diagram that indicates.
Fig. 4 is technology disclosed according to the present invention, indicates the square signal of each unit in chromosome scanning imaging system
Figure.
Fig. 5 is technology disclosed according to the present invention, indicates the inspection obtained after splicing as captured by 1.25 times of objective scans
Survey the schematic diagram of the image of the main distributed areas of object.
Fig. 6 is technology disclosed according to the present invention, is indicated by focusing point range selection when 10 times of scanning in identification region
It is inside focused, obtains the schematic diagram of the focal plane of entire slide.
Specific embodiment
In order to make the purpose of the present invention, technical characteristic and advantage, can more correlative technology field personnel understood, and be able to
Implement the present invention, cooperates appended schema herein, specifically illustrates technical characteristic and embodiment of the invention, and enumerate preferable reality
Apply example further explanation.It is not also needed with the schema hereinafter compareed to express signal related with feature of present invention
It is completely drawn according to practical situation.And the technology well-known to those skilled in the art involved in the explanation of this case embodiment
Content is also no longer stated.
It please also refer to Fig. 1 and Fig. 2 first.Fig. 1 and Fig. 2 is the schematic diagram for indicating chromosome scanning imagery board.In Fig. 1
And in Fig. 2, chromosome scanning imagery board 1 includes preceding box body combinations 10, rear box combination 12, storage assembled unit 14, basis
Assembly unit 16, microscopic system 18, microscope way moving objective table 20, oil supply system 22, overall control system 24 and
Oil bottle component 26, in addition, it be board 1 further includes triaxial linkage mechanical hand arm 28, circuit main board that the chromosome in Fig. 2, which is scanned into,
30 and scanning element 32, other components in Fig. 2 are identical as Fig. 1, to avoid any confusion, therefore repeat no longer in Fig. 2
Mark.Wherein, rear box combination 10, storage assembled unit 14, microscopic system 18 and company are equal for all components of system 28
It is installed in the big plane of infrastructure component unit 16；Store assembled unit 14, microscopic system 18, oil supply system 22 and totality
Control system 24 is set to the left side of infrastructure component unit 16, and scanning element 32 is set on microscope way moving objective table 20
On the microscope frame (not shown in the figure) of side.Circuit main board 30 is then to be set to rear box combination for circuit control device
In 12.
It please also refer to Fig. 3 and Fig. 4.Fig. 3 is that the chromosome scanning imagery board according to represented by Fig. 1 and Fig. 2 is further
Each unit in chromosome scanning imaging system is indicated with chromosome scanning imaging system come the block schematic diagram and Fig. 4 indicated
Block schematic diagram.Herein it is noted that having part component same as the prior art in Fig. 1 and Fig. 2, swept to focus on chromosome
Retouch imaging system, therefore illustrate only for component related with chromosome scanning imaging system.
In Fig. 3, chromosome scanning imaging system 4 includes slide film feeding apparatus 40, microscopic system 42, overall control system
System 44.Wherein, the microscopic system 42 in Fig. 3 is microscopic system 18 disclosed in Fig. 1, but is understood for convenience,
The following description is then the camera unit with microscopic system 42 to indicate, scanning element 32 is then comparable to indicate in Fig. 3
424.It is to be described in detail for each unit of whole chromosome scanning imaging system 4 and operation below, in explanation
Cooperate Fig. 1 and Fig. 2, together also simultaneously in order to understand the operation of whole chromosome scanning imaging system 4.
As shown in figure 3, in the present invention, slide film feeding apparatus 40 has multiple slide boxs 402, with one of slide box
For 402, there are multiple slides 4022, and carry detectable substance 40222 on each slide 4022 in a slide box 402.
With the present invention more preferably for embodiment, slide film feeding apparatus 40 at least have there are four slide box 402, each slide box
There are 40 slides 4022 in 402, therefore, 4022 quantity of slide for carrying out microscopic each time and being scanned and taking pictures
At least 160.
Microscopic system 42 includes microscope way moving objective table 422, camera unit 424, optical path unit 426 and object
Mirror unit 428, wherein microscope way moving objective table 422 is same with the microscope way moving objective table 20 in Fig. 1
Component, to carry the slide 4022 with detectable substance 40222.Camera unit 424 is for every in slide film feeding apparatus 40
Detectable substance 40222 on a piece of slide 4022 is scanned and the image of shot detection object 40222.Optical path unit 426 is whole
The total collection of the optical module of a microscopic system 42, to provide light source irradiation, to be placed on 40 microscope of microscopic system two-way
Detectable substance 40222 and object lens 428 on moving stage 422 are made of the object lens of three different multiples, wherein
Object lens 428 are the detectable substances 40222 that microscope way moving objective table 422 is seated in for observing.It is of the invention compared with
In preferred embodiment, the object lens of three different multiples of object lens 428 be respectively 1.25 times of object lens with minimum multiple,
10 times of object lens of the second multiple and 100 multiple object lens of highest multiple, for object lens 428 practical operation step under
Face is described in detail.In addition it is noted that the object lens in above-mentioned contained minimum multiple, the second multiple and highest multiple are in the present invention
Used object lens make reference, and are to indicate in three object lens of the present invention, and 1.25 multiples are minimum multiple, 10
Multiple is the second multiple and 100 multiples are highest multiples, does not mean that minimum or most high power in all microscopical object lens
Number.
Overall control system 44 has even for system 442, wherein connecting for system 442 is by X-Y-Z triaxial linkage mechanical hand
Arm (not shown in the figure) is formed, and the Y-axis in X-Y-Z triaxial linkage mechanical hand arm is basic axis so that even for system 442 with
Front and rear direction movement, Z axis carries out left and right direction movement in Y-axis with upper and lower direction movement and X-axis on Z axis, even for being
System 442 is controlled by overall control system 44 and is picked and placed on microscope way moving objective table 422 from slide film feeding apparatus 40
Slide 4022, in an embodiment of the present invention, signified front, rear, left and right direction refer to user station such as Fig. 1 or Fig. 2
The relative direction of 1 front of chromosome scanning imagery board.
Therefore according to above-mentioned, overall control system 44 is using even for the X-Y-Z triaxial linkage mechanical hand arm of system 442 with list
Then the mode that piece even supplies is seated in by taking out slide 4022 in slide box 402 numbered in slide film feeding apparatus 40 aobvious
On micro mirror way moving objective table 422, scanning is completed to the detectable substance 40222 on slide 4022 and takes pictures and then utilize company
For system 442 X-Y-Z triaxial linkage mechanical hand arm by slide 4022 by being removed on microscope way moving objective table 422, then
The a piece of slide 4022 to be scanned and be taken pictures under placement.Slide 4022 is continuously picked and placed aobvious using slide film feeding apparatus 40
Advantage on micro mirror way moving objective table 422 is, does not need to carry out via artificial, can also be with other than saving manpower
Avoid polluteing the detectable substance 40222 on slide 4022 when manual operation, and the result that causes to scan and take pictures has error, separately
Outside, it can be scanned and take pictures for a large amount of detectable substance 40222 using slide film feeding apparatus 40, can obtain in a short time
Obtain the result of a large amount of detectable substances 40222.
In addition, fortune of the overall control system 44 to control each of whole chromosome scanning system 4 hardware component
Make, wherein overall control system 44 controls the movement of microscope way moving objective table 422, so that microscope way moving loading
Platform 422 is in a first direction and second direction moves, and controls object lens 428 in third direction by microscopic system 42
Movement come control microscope way moving objective table 422 with object lens 428 third direction move, wherein first direction,
Signified X-direction, Y-direction and Z-direction on the drawing in Fig. 1 or Fig. 2 that second direction and third direction refer respectively to.
In addition, overall control system 44 controls object lens 428 by the object lens with minimum multiple to having highest multiple
Object lens sequentially the detectable substance 40222 of the slide 4022 on microscope way moving objective table 422 is observed, and
While observing detectable substance 40222, overall control system 44 controls the camera unit 424 in microscopic system 42 to scan aobvious
The detectable substance 40222 on slide 4022 on micro mirror way moving objective table 422, and by the object lens with minimum multiple
(1.25 multiple object lens) are scanned detectable substance 40222 and judge whether to need to be swept using three-wheel by overall control system 44
It retouches, the mode of scanning is as described below:
First wheel scan uses 1.25 times of object lens, i.e., when the triaxial linkage mechanical hand arm 28 of slide film feeding apparatus 40 will have
After the slide 4022 of detectable substance 40222 takes out from slide box 402, it is seated on microscope way moving objective table 422, it is micro-
The optical path unit 426 of mirror system 42 is to the slide (glass with detectable substance 40222 on microscope way moving objective table 422
Piece) 4022 offers and are irradiated light sources, and allow camera unit 424 through the object lens of minimum multiple (1.25 times) to micro-
The detectable substance 40222 of slide 4022 on mirror way moving objective table 422 carries out entire scan, obtains detectable substance 40222 and is distributed
Region.
Then, the second wheel scan is carried out, at this point, overall control system 44 controls object lens 428 for next multiple
Object lens, 10 times of object lens, in alignment with the detectable substance 40222 of the slide 4022 on microscope way moving objective table 422,10 times at this time
Object lens can be first directed in aforementioned first round scanning step, while 1.25 times of object lens 442 are observed, camera unit 424
The point scanned in the region that obtained detectable substance 40222 is distributed is focused, to obtain 10 times of cokes of the detectable substance 40222
Then plane carries out full sheet (i.e. to the detectable substance 40222 on full wafer slide 4022) and is scanned, and to 10 times of each of acquisition
The detectable substance 40222 of image be ranked up and position.Then third wheel scan is carried out again, and third wheel scan is then at 100 times
The high definition pattern of detectable substance 40222 is obtained when object lens are observed using camera unit 424.
In addition, when carrying out third wheel scan each time, i.e., observing detection using 100 times of object lens in the present invention
Object 40222 and when being scanned by camera unit 424, overall control system 44 can control oil supply system 22 and oil bottle component 26
(as shown in Figure 1) can provide arborvitae oil droplet on slide 4022, and the purpose using cedar oil is the amplification because of 100 times of object lens
Multiple is high, but lens very little, when light passes through medium substance (such as slide, air, object lens) of different densities, part light
Can occur to reflect and scatter and disappear, the light into lens barrel is few, and the visual field is darker, detectable substance 40222 observe it is unclear, therefore can be in object lens
It is added dropwise between slide 4022 and the similar cedar oil (refractive index 1.515) of glass refraction (1.52), so that into oil mirror
Light increase, field luminance enhancing detectable substance 40222 is clearly observed.
It is noted that in an embodiment of the present invention, camera unit 424 is obtaining the detection on per a piece of slide 4022
The scanning of the high-definition image of object 40222 is to be carried out by the object lens of low power number to the object lens of high magnification numbe, first by 1.25 times of object lens
It obtains the region that detectable substance 40222 is distributed, then obtains 10 times of focal planes and comprehensive scanning by 10 times of object lens, and by totality
Control system 44 is ranked up and positions, and is finally observed again by 100 times of object lens and obtains the high definition figure of detectable substance 40222
Picture.It is noted that the mode that overall control system 44 is ranked up obtained image is the spy according to detectable substance 40222
Collection is closed to judge, by taking detectable substance 40222 is chromosome as an example, then the foundation of its sequence is exactly length, the dye according to chromosome
The features such as the area of the number of colour solid and chromosome are come integrated ordered.
In addition, not will start object lens 428 then when not needing to determine scanning area and carry out three wheel scans, that is, be not required to
The first wheel scan is carried out using 1.25 times of object lens.And in the present invention, it is discussed primarily directed to three-wheel scan pattern,
Therefore it is not stated herein for not needing the step of carrying out three-wheel scan pattern.
In addition, in the present invention, when not needing to carry out three-wheel scan pattern, then the object lens that can use general multiple carry out
Scanning, and focus to take pictures under 100 times and take the image of detectable substance 40222, but if desired carry out three-wheel scan pattern, then overall control
System 44 processed carries out three-wheel scan pattern according to aforesaid way.It determines whether to progress three-wheel scan pattern and is whether use
Full sheet contaminates piece, and drop, which contaminates piece, will use three wheel scans, and full sheet dye piece can not use three wheel scans.
According to the above, for example the process of bright three-wheel scan pattern, carries out first using 1.25 times of object lens first
Wheel scan carries out entire scan for the image of the detectable substance 40222 on slide 4022 and carries out image using camera unit 424
Shooting, thus according to its scanning sequency by the image mosaic taken at a Zhang great Tu, as shown in Figure 5.Pass through figure on big figure
As the main distributed areas of processing and identification acquisition detectable substance 0222, detectable substance 40222 is chromosome in this embodiment.
Then, the second wheel scans are carried out using 10 times of object lens, according to the 10 of slide 4022 times of focal planes and current micro-
X, the Y location of mirror system 42 calculate the height of microscope Z-direction (object lens vertical direction) and are moved to the position acquisition image,
It moves synchronously as shown in fig. 6, overall control system 44 controls X, Y, Z to acquire image, dye is obtained from each 10 times of images
Colour solid 40222 is simultaneously ranked up, and supplies 100 times of object lens (or oil mirror observation).
And then, overall control system control oil supply system 22 and oil bottle component 26 provide oil dripping on slide 4022, to
After the completion of oil dripping, operation of putting the oil is completed by 100 times of object lens.It is overall to control according to the chromosome 40222 of the forward position of 10 times of sequences
System 44 processed is obtained clearly image and is taken pictures using camera unit 424 to the position by focusing, which can carry out
Primary a wide range of focusing and primary small-scale focusing can finally export 100 times of 446 images of object lens for diagnosing and/or dividing
Analysis uses.
The foregoing is merely the preferred embodiments of the invention, the interest field that is not intended to limit the invention；More than simultaneously
Description, the special personage of correlative technology field should can be illustrated and be implemented, thus other without departing from it is disclosed it
The lower equivalent change or modification completed of spirit, should be included in claim.
Claims (10)
1. a kind of chromosome scanning imaging system, which is characterized in that the chromosome scanning imaging system includes:
Slide film feeding apparatus, the slide film feeding apparatus include multiple slide boxs, have multiple slides in each slide box
And there is detectable substance on each slide；
Microscopic system includes:
Microscope way moving objective table, the microscope way moving objective table have described in the determinand to carrying tool
Slide；
Camera unit, the camera unit carry out the detectable substance on each slide in the slide film feeding apparatus
Scan and shoot the image of the detectable substance；
Optical path unit, the optical path unit provide the microscope way moving that light source irradiation is placed on the microscopic system
The detectable substance on objective table；And
Object lens, the object lens are made of the object lens of three different multiples, and each object lens are put to observe
The detectable substance on the slide on the microscope way moving objective table；And
Overall control system, the overall control system have even for system, and the company is mechanical by X-Y-Z three-shaft linkage for system
Arm forms, and the Y-axis in the X-Y-Z triaxial linkage mechanical hand arm is basic axis, so that the company is for system with forward and backward
Direction movement, the Z axis carry out left and right direction in the Y-axis with upper and lower direction movement and the X-axis on the Z axis
Movement, the company are controlled by the overall control system by picking and placing the slide described in the slide film feeding apparatus for system
On microscope way moving objective table, wherein the overall control system further include:
Control the microscope way moving stage movement so that the microscope way moving objective table in a first direction and
Second direction movement, and it is described aobvious to control in the movement of third direction by the microscopic system to control the object lens
Micro mirror way moving objective table is moved with the object lens in the third direction；
Control the object lens by the object lens with minimum multiple to the object lens with highest multiple sequentially to
The detectable substance on the slide on the microscope way moving objective table is observed；
The overall control system controls the scanning of the camera unit in the microscopic system and carries in microscope way moving
The detectable substance on the slide on object platform, and the detectable substance is judged by the object lens with the minimum multiple
Whether three wheel scans are used, existed if so, the overall control system controls the camera unit in the microscope unit
Sequentially with the object lens with the minimum multiple to having the object lens of the highest multiple to observe the detectable substance
The detectable substance is scanned with three-wheel continuation mode simultaneously, wherein
The overall control system controls the camera unit and executes the first wheel scan, the camera unit for have it is described most
The object lens of small multiple carry out entire scan for the detectable substance on the slide and are distributed with obtaining the detectable substance
Region；
The overall control system controls the camera unit and executes the second wheel scan, and the scanning element utilizes the second multiple
The object lens are observed and are selected point in the region of the detectable substance distribution and are focused to obtain described the
Focal plane under diploidy number, and full sheet scanning is carried out to obtain multiple images under second multiple, and by the totality
The detectable substance of described image of the control system for obtaining under second multiple is ranked up and positions；And
The overall control system controls the camera unit and executes third wheel scan, and the scanning element utilizes highest multiple
The object lens focus shooting according to positioning for the forward detectable substance that sorts and obtain a series of high-definition images.
2. chromosome scanning imaging system as described in claim 1, which is characterized in that the slide is even for there are four unit tools
Slide box, and there are 40 slides in each slide box.
3. chromosome scanning imaging system as described in claim 1, which is characterized in that the detectable substance is chromosome or thin
Born of the same parents.
4. chromosome scanning imaging system as described in claim 1, which is characterized in that the microscopic system also includes judgement
Unit is to judge whether the detectable substance uses three wheel scans.
5. chromosome scanning imaging system as described in claim 1, which is characterized in that the overall control system also includes confession
Oil system and oil bottle component.
6. chromosome scanning imaging system as claimed in claim 5, which is characterized in that described in being utilized when the scanning element most
Before the object lens of high magnification numbe take pictures for the detectable substance to obtain the high definition pattern, the overall control system is by institute
It states and takes out oil in oil supply system and the oil bottle component and the oil bottle component to drip on the object lens with the highest multiple.
7. chromosome scanning imaging system as claimed in claim 6, which is characterized in that the object with the highest multiple
Mirror is 100 times of object lens.
8. chromosome scanning imaging system as described in claim 1, which is characterized in that the object with the minimum multiple
Mirror is 1.25 times of object lens, the object lens with second multiple are 10 times of object lens and the object with the highest multiple
Mirror is 100 times of object lens.
9. chromosome scanning imaging system as described in claim 1, which is characterized in that the overall control system further includes row
Sequence unit is ranked up and positions to the detectable substance of the described image for obtaining under second multiple.
10. chromosome scanning imaging system as claimed in claim 9, which is characterized in that the sequencing unit is according to each
The area of the length of the detectable substance, the number of each detectable substance or each detectable substance is ranked up.
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|---|---|---|---|
| CN201811633191.XA CN110361382A (en) | 2018-12-29 | 2018-12-29 | A kind of chromosome scanning imaging system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811633191.XA CN110361382A (en) | 2018-12-29 | 2018-12-29 | A kind of chromosome scanning imaging system |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111045203A (en) * | 2020-01-15 | 2020-04-21 | 深圳市生强科技有限公司 | Objective lens combination and full-glass digital acquisition system |
| CN111220510A (en) * | 2020-01-15 | 2020-06-02 | 深圳市生强科技有限公司 | Full-automatic blood cell morphology scanning system |
| CN115079396A (en) * | 2022-08-19 | 2022-09-20 | 杭州德适生物科技有限公司 | Chromosome karyotype analysis microscopic shooting device and method based on 100-fold objective lens |
| CN117147551A (en) * | 2023-10-30 | 2023-12-01 | 中国科学院苏州生物医学工程技术研究所 | Chromosome scanning imaging method and device |
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