CN110339133A - A kind of preparation method of green bacteriostatic hand sanitizer - Google Patents
A kind of preparation method of green bacteriostatic hand sanitizer Download PDFInfo
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- CN110339133A CN110339133A CN201910776050.1A CN201910776050A CN110339133A CN 110339133 A CN110339133 A CN 110339133A CN 201910776050 A CN201910776050 A CN 201910776050A CN 110339133 A CN110339133 A CN 110339133A
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- China
- Prior art keywords
- preparation
- extract
- extraction
- hibisci mutabilis
- flos hibisci
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- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 230000003385 bacteriostatic effect Effects 0.000 title claims abstract description 14
- 239000000284 extract Substances 0.000 claims abstract description 30
- 241000628997 Flos Species 0.000 claims abstract description 26
- 244000205754 Colocasia esculenta Species 0.000 claims abstract description 24
- 235000006481 Colocasia esculenta Nutrition 0.000 claims abstract description 24
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 22
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000000605 extraction Methods 0.000 claims abstract description 16
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims abstract description 13
- 229930003944 flavone Natural products 0.000 claims abstract description 13
- 235000011949 flavones Nutrition 0.000 claims abstract description 13
- 229930183111 sapindoside Natural products 0.000 claims abstract description 13
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000001509 sodium citrate Substances 0.000 claims abstract description 11
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 11
- 239000000126 substance Substances 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 108010059892 Cellulase Proteins 0.000 claims description 16
- 108090000790 Enzymes Proteins 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 16
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 claims description 16
- 229940106157 cellulase Drugs 0.000 claims description 16
- 229940088598 enzyme Drugs 0.000 claims description 16
- 240000003377 Shepherdia canadensis Species 0.000 claims description 15
- 235000018324 Shepherdia canadensis Nutrition 0.000 claims description 15
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 claims description 13
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 13
- 239000003208 petroleum Substances 0.000 claims description 13
- 230000009849 deactivation Effects 0.000 claims description 12
- 239000011347 resin Substances 0.000 claims description 11
- 229920005989 resin Polymers 0.000 claims description 11
- 238000000498 ball milling Methods 0.000 claims description 10
- 235000019441 ethanol Nutrition 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 239000000287 crude extract Substances 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 8
- 235000012054 meals Nutrition 0.000 claims description 6
- 230000002829 reductive effect Effects 0.000 claims description 6
- 102000004139 alpha-Amylases Human genes 0.000 claims description 5
- 108090000637 alpha-Amylases Proteins 0.000 claims description 5
- 229940024171 alpha-amylase Drugs 0.000 claims description 5
- 108010089934 carbohydrase Proteins 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 5
- 239000000344 soap Substances 0.000 claims description 5
- 238000001179 sorption measurement Methods 0.000 claims description 5
- 239000002250 absorbent Substances 0.000 claims description 4
- 230000002745 absorbent Effects 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 230000006837 decompression Effects 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 150000002212 flavone derivatives Chemical class 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 238000000967 suction filtration Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 238000007605 air drying Methods 0.000 claims description 2
- 238000002604 ultrasonography Methods 0.000 claims 2
- 229930182478 glucoside Natural products 0.000 claims 1
- 150000008131 glucosides Chemical class 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 12
- -1 flavone compound Chemical class 0.000 abstract description 10
- 150000004676 glycans Chemical class 0.000 abstract description 10
- 229920001282 polysaccharide Polymers 0.000 abstract description 10
- 239000005017 polysaccharide Substances 0.000 abstract description 10
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 9
- 239000004094 surface-active agent Substances 0.000 abstract description 9
- 230000036961 partial effect Effects 0.000 abstract description 6
- 239000004909 Moisturizer Substances 0.000 abstract description 4
- 239000000022 bacteriostatic agent Substances 0.000 abstract description 4
- 239000003599 detergent Substances 0.000 abstract description 4
- 238000004137 mechanical activation Methods 0.000 abstract description 4
- 230000001333 moisturizer Effects 0.000 abstract description 4
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 abstract description 3
- 208000028990 Skin injury Diseases 0.000 abstract description 3
- ICYQEHVSYKEXEN-UHFFFAOYSA-M azanium 1-hexadecylpyridin-1-ium dichloride Chemical compound [Cl-].C(CCCCCCCCCCCCCCC)[N+]1=CC=CC=C1.[Cl-].[NH4+] ICYQEHVSYKEXEN-UHFFFAOYSA-M 0.000 abstract description 3
- 239000007853 buffer solution Substances 0.000 abstract description 3
- 239000003002 pH adjusting agent Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- SNRUBQQJIBEYMU-UHFFFAOYSA-N dodecane Chemical compound CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229930182490 saponin Natural products 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000192125 Firmicutes Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- CHTAZMKEXNOPNA-UHFFFAOYSA-N azane;pyridin-1-ium;chloride Chemical compound N.[Cl-].C1=CC=[NH+]C=C1 CHTAZMKEXNOPNA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Emergency Medicine (AREA)
- Detergent Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to detergent applications, disclose a kind of preparation method of green bacteriostatic hand sanitizer, the present invention extracts the flavone compound in Flos Hibisci Mutabilis in such a way that mechanical activation is in conjunction with ultrasonic extraction, mechanical lapping is conducive to for substance to be ground to thinner partial size, and ultrasonic extraction extraction effect when partial size is smaller is more preferable, two ways combines, and facilitates high efficiency extraction.The present invention using flavone compound there is good antibacterial bacteriostatic performance to substitute the bacteriostatic agent in traditional hand cleanser, the surfactants such as cetyl pyridinium ammonium chloride, lauryl sodium sulfate in traditional hand cleanser are substituted using the sapindoside with natural surfactant characteristic, moisturizer is substituted using the taro polysaccharide with excellent performance of keeping humidity, using citric acid and sodium citrate buffer solution as pH adjusting agent, it is prepared for a kind of new hand sanitizer.It is smaller to human skin injury while having effects that traditional hand cleanser stain removal bactericidal, it is a kind of hand cleanser of green high-efficient.
Description
Technical field
The present invention relates to detergent applications more particularly to a kind of preparation methods of green bacteriostatic hand sanitizer.
Background technique
As the improvement of people's living standards, consumption idea is also changing, wash one's hands used in people's daily life
Product has gradually turned to hand cleanser by soap and perfumed soap.Hand cleanser is that the common shield of washing of one kind is used in people's daily life
Product, hand cleanser has apparent advantage compared with traditional solid soap, not only easy to use, but also can avoid the secondary dirt of bacterium
Dye.But the ingredient in presently commercially available hand cleanser contains the chemical component being harmful to the human body, and natural plant component has heat-clearing
The effect of removing toxic substances, anti-bacteria wet keeping, and the advantage that medicinal herb components have toxic side effect small, mild, inexpensive, source is wide, having
While good antibacterial, moisturizing, antioxidant effect, the injury to hand skin can also be reduced, therefore wash to new medicine
The research of hand liquid has certain practical value.
Flavone compound has good inhibiting effect to some pathogenic microorganisms, for gram-positive bacteria, gram
Negative bacterium, fungi and virus have different degrees of inhibiting effect, in addition, it, which also can be reduced free radical, forms and remove free radical
Antioxidant activity, there is lipoid peroxidization resistant, be a kind of natural organic oxidation-resistant agent.Soapberry in timbol
Saponin(e is natural surfactant, can be with 100% degradation, with the detergent that sapindoside is raw material production than on the market
Non-phosphate detergent is more effectively in the protection of ecological environment.Polysaccharide is present in plant cell wall, belongs to polarity macromolecular substances, closely
Nian Lai, a small amount of research start to concentrate on the performance of keeping humidity of taro polysaccharide, have experiment to show that its moistening effect is better than traditional moisturizer
Propylene glycol and glycerine.
Summary of the invention
In order to solve the above-mentioned technical problems, the present invention provides a kind of preparation method of green bacteriostatic hand sanitizer, the present invention
Relatively mild hand cleanser is prepared for a kind of economically viable means.The present invention uses side of the mechanical activation in conjunction with ultrasonic extraction
Formula extracts the flavone compound in Flos Hibisci Mutabilis, and mechanical lapping is conducive to for substance to be ground to thinner partial size, and ultrasonic extraction
When partial size is smaller, extraction effect is more preferable, and two ways combines, and facilitates high efficiency extraction.The present invention extracts Huang from herbaceous plant
Ketone compounds, sapindoside, taro polysaccharide, extracting mode economical and effective.The present invention has good using flavone compound
Good antibacterial bacteriostatic performance substitutes the bacteriostatic agent in traditional hand cleanser, utilizes the soapberry soap with natural surfactant characteristic
Glycosides substitutes the surfactants such as cetyl pyridinium ammonium chloride, lauryl sodium sulfate in traditional hand cleanser, using with excellent
The taro polysaccharide substitution moisturizer of performance of keeping humidity is prepared for one kind using citric acid and sodium citrate buffer solution as pH adjusting agent
New hand sanitizer.It is smaller to human skin injury while having effects that traditional hand cleanser stain removal bactericidal, it is a kind of green
Efficient hand cleanser.
The specific technical proposal of the invention is: a kind of preparation method of green bacteriostatic hand sanitizer, in terms of g and mL, including with
Lower step:
1) dry Flos Hibisci Mutabilis is taken, petroleum ether is added, decoloration is impregnated in water bath with thermostatic control.
The present invention impregnates Flos Hibisci Mutabilis with petroleum ether first, is still bleached, and prevents from making its color to when being applied to hand cleanser
At negative consequence.
2) mixture of petroleum ether and Flos Hibisci Mutabilis in step 1) is transferred in ball mill, it is 6-15mm's that diameter, which is added,
Different zirconia ball ball milling 20-30min.
3) mixed liquor after ball milling in step 2) is subjected to decompression suction filtration, ethyl alcohol and water will be used to impregnate after filter residue and drying, surpassed
Sound extracts, and obtains Flos Hibisci Mutabilis extract.
Ultrasonic extracting method has the advantages that without heating, periodical, low energy consumption, recovery rate is high, but requires sample particle diameter
It is sufficiently small to obtain preferable extraction effect, and mechanical lapping helps that agent particle size is made to become small as far as possible, the present invention
By mechanical activation in conjunction with ultrasonic extraction, to improve the recovery rate of flavone compound.
4) supernatant after centrifugation in step 3) is concentrated under reduced pressure, it is stand-by obtains Flos Hibisci Mutabilis flavone extract.
5) it by the fresh taro and soapberry pericarp of cleaning peeling, is crushed after forced air drying stand-by.
6) it weighs soapberry powder obtained by step 5) and container is placed in 1g: 5-10mL solid-to-liquid ratio using distilled water as solvent
In, add cellulase and pectase, constant temperature extracts, and carries out high temperature enzyme deactivation to crude extract.
7) macroporous absorbent resin ethyl alcohol is impregnated and is washed to eluate and generated without white opacity, then is successively molten with NaOH
Liquid, HCl solution impregnate, and are washed till neutrality.
8) macroporous resin adsorption of crude extract step 7) obtained by step 6) is filtered after purification, obtains no trouble after dry
Sub- saponin extract, for use.
The present invention carries out adsorption and purification to gained sapindoside crude extract using macroreticular resin, avoids impurity to washing one's hands
The adverse effect of fluidity energy.
9) it weighs taro meal 8-12g to be placed in container, addition 150-200mL distilled water, after isothermal vibration 2-3h, uses lemon
Sour sodium adjusts pH value, and cellulase and pectase, ultrasonic extraction 10-15min is added.
10) 20-25U/g α-amylase is first added into extracting solution obtained by step 9), in 70-80 DEG C, the condition of pH=6-6.5
Lower enzymatic hydrolysis, adds the carbohydrase of 20-25U/g, then is digested, and taro polyoses extract is dried to obtain after high temperature enzyme deactivation, to
With.
The higher ingredient of content is starch in taro, and the present invention successively carries out enzyme to crude extract with α-amylase and carbohydrase
Solution, primarily to removal starch.
11) gained Flos Hibisci Mutabilis extract 0.3-0.5g in step 3), sapindoside extract 10- obtained by step 8) are taken
15g, step 10) gained taro polyoses extract 1-2g, daily chemical essence 0.1-0.3g are added pure water to 100-150mL, heat molten
Solution, is cooled to room temperature after dissolution, and adjusting pH with citric acid and sodium citrate is 6.5-7 to get green bacteriostatic hand sanitizer.
The present invention uses the flavone compound with good restraining and sterilizing bacteria performance to substitute bacteriostatic agent in traditional hand cleanser, utilizes
The sapindoside of characteristic with natural surfactant substitutes cetyl pyridinium ammonium chloride in common hand cleanser, dodecane
The surfactants such as base sodium sulphate use the taro polysaccharide with excellent performance of keeping humidity as moisturizer, wash one's hands with tradition
While the effect of liquid stain removal bactericidal cleans, and it is relatively mild, human skin is injured smaller.
Preferably, the mass ratio of petroleum ether and Flos Hibisci Mutabilis is 5-7: 1 in step 1), water bath with thermostatic control temperature is 60-70
DEG C, soaking time 80-120min.
Preferably, the zirconia ball diameter of addition is at least three kinds of in step 2), the zirconia ball heap volume of addition is
300-400mL；Drum's speed of rotation is 300-450r/min, Ball-milling Time 20-30min.
Preferably, in step 3), concentration of alcohol 65-75wt%, solid-to-liquid ratio is 1: 20-1: 30 (g/ when ultrasonic extraction
ML), ultrasonic extraction power is 500-600W, extraction time 28-35min.
Preferably, the mass ratio of cellulase and pectase is 1: 0.8-1.2, and additional amount is soapberry in step 6)
The 0.1-0.3% of powder quality；Constant temperature Extracting temperature is 50-60 DEG C, extraction time 2.5-4h, and the high temperature enzyme deactivation is boiling water
Bathe 5-10min enzyme deactivation.
Preferably, in step 7), the concentration of the ethyl alcohol is 90-95wt%, the NaOH solution and HCl solution it is dense
Degree is 3-5wt%, soaking time 2-4h.
Preferably, the macroreticular resin is AB-8, CAD-40 or DM 301 in step 7).
Preferably, the mass ratio of cellulase and pectase is 1.3-1.7: 1, and additional amount is taro meal in step 9)
The 1.2-1.5% of quality.
Preferably, in step 9), adjusting pH with sodium citrate is 6-6.5, ultrasonic extraction 10-15min, and ultrasonic extraction is solid
Liquor ratio is 1: 20-25g/mL.
Preferably, in step 10), enzymolysis time 1.5h-2h.
Be compared with the prior art, the beneficial effects of the present invention are: the present invention with a kind of economically viable means be prepared for compared with
Mild hand cleanser.The present invention extracts the flavonoid in Flos Hibisci Mutabilis in such a way that mechanical activation is in conjunction with ultrasonic extraction
Object, mechanical lapping is conducive to for substance to be ground to thinner partial size, and ultrasonic extraction extraction effect when partial size is smaller is more preferable, and two
Kind mode combines, and facilitates high efficiency extraction.The present invention extracts flavone compound, sapindoside, taro from herbaceous plant
Polysaccharide, extracting mode economical and effective.The present invention using flavone compound there is good antibacterial bacteriostatic performance substitution tradition to wash
Bacteriostatic agent in hand liquid substitutes cetyl in traditional hand cleanser using the sapindoside with natural surfactant characteristic
The surfactants such as pyridine ammonium chloride, lauryl sodium sulfate substitute moisturizing using the taro polysaccharide with excellent performance of keeping humidity
Agent is prepared for a kind of new hand sanitizer using citric acid and sodium citrate buffer solution as pH adjusting agent.There is traditional hand cleanser
It is smaller to human skin injury while the effect of stain removal bactericidal, it is a kind of hand cleanser of green high-efficient.
Specific embodiment
The present invention will be further described with reference to the examples below.
Embodiment 1
1) dry Flos Hibisci Mutabilis 5g is taken, appropriate petroleum ether is added, makes petroleum ether: mass ratio=6 of Flos Hibisci Mutabilis: 1, it is Celsius 65
85min is impregnated in the thermostat water bath of degree carries out decolorization；
2) mixture of petroleum ether and Flos Hibisci Mutabilis in step 1) being transferred in ball mill, addition diameter is 6mm,
10mm, 15mm zirconia ball 300mL (heap volume) are with the revolving speed ball milling 20-30min of 350r/min；
3) mixed liquor after ball milling in step 2) is subjected to decompression suction filtration, the second of 65wt% will be used after obtained filter residue and drying
Alcohol impregnates 20min, is that 1: 20 (g/mL) carries out ultrasonic extraction with solid-to-liquid ratio, ultrasonic extraction power is 500W, extraction time
28min；
4) supernatant after centrifugation in step 3) is concentrated under reduced pressure, it is stand-by obtains Flos Hibisci Mutabilis flavone extract；
5) it by the fresh taro and soapberry pericarp of cleaning peeling, is waited for respectively at crushed after being dried abundant in air dry oven
With；
6) the suitable soapberry powder of precise uses distilled water to be placed in three mouthfuls as solvent with 1: 5g/mL solid-to-liquid ratio
In flask, the mixed enzyme of cellulase and pectase is added to extract 2.5h, the quality of cellulase and pectase at 50 DEG C
Than being 1: 1, additional amount is the 0.1% of substrate soapberry powder quality, after the completion of extraction, boiling water bath 5min enzyme deactivation；
7) AB-8 macroporous absorbent resin is impregnated with the ethyl alcohol of 90wt% and is washed to eluate and generated without white opacity, then
2h successively is impregnated with 4% NaOH, HCl solution, is washed till neutrality；
8) macroporous resin adsorption obtained in the crude extract step 7) in step 6) is filtered after purification, is dried to obtain
Sapindoside powder is stand-by；
9) it weighs taro meal 10g to be placed in 300mL flask, 150 distilled water of addition, after isothermal vibration 2h, uses sodium citrate
Adjusting pH value is 6, addition cellulase and pectase, and the mass ratio of cellulase and pectase is 1.5: 1, and additional amount is
The 1.2% of amount of substrate, liquid-solid ratio 20: 1, ultrasonic extraction 10min；
10) 20U/g α-amylase is first added to extracting solution obtained in step 9), 75 DEG C, is digested under conditions of 6 in pH
1.5h adds the carbohydrase of 20U/g, then carries out enzymatic hydrolysis 1.5h, and it is stand-by that taro polysaccharide is dried to obtain after high temperature enzyme deactivation；
11) Flos Hibisci Mutabilis extract 0.3g obtained in step 3), step 8) sapindoside extract 10g, step 10) are taken
Middle polyoses extract 1g, daily chemical essence 0.1g are added pure water to 100mL, dissolve by heating, be cooled to room temperature after dissolution, use lemon
It is 6.5 to get a kind of green bacteriostatic hand sanitizer that acid and sodium citrate, which adjust pH,.
Embodiment 2
1) dry Flos Hibisci Mutabilis 8g is taken, appropriate petroleum ether is added, makes petroleum ether: Flos Hibisci Mutabilis mass ratio=6: 1, at 65 degrees Celsius
Thermostat water bath in impregnate 90min carry out decolorization；
2) mixture of petroleum ether and Flos Hibisci Mutabilis in step 1) is transferred in ball mill, be added diameter be 6mm, 8mm,
1o mm zirconia ball 300mL (heap volume) is with the revolving speed ball milling 25min of 400r/min；
3) mixed liquor after ball milling in step 2) is subjected to decompression suction filtration, the second of 70wt% will be used after obtained filter residue and drying
Alcohol impregnates 25min, is that 1: 20 (g/mL) carries out ultrasonic extraction with solid-to-liquid ratio, ultrasonic extraction power is 600W, extraction time
28min；
4) supernatant after centrifugation in step 3) is concentrated under reduced pressure, it is stand-by obtains Flos Hibisci Mutabilis flavone extract；
5) it by the fresh taro and soapberry pericarp of cleaning peeling, is waited for respectively at crushed after being dried abundant in air dry oven
With；
6) the suitable soapberry powder of precise uses distilled water to be placed in three mouthfuls as solvent with 1: 6g/mL solid-to-liquid ratio
In flask, the mixed enzyme of cellulase and pectase is added to extract 3h, the mass ratio of cellulase and pectase at 55 DEG C
It is 1: 1, additional amount is the 0.1% of substrate soapberry powder quality, after the completion of extraction, boiling water bath 8min enzyme deactivation；
7) CAD-40 macroporous absorbent resin is impregnated with the ethyl alcohol of 95wt% and is washed to eluate and generated without white opacity,
2.5h successively is impregnated with 4% NaOH, HCl solution again, is washed till neutrality；
8) macroporous resin adsorption obtained in the crude extract step 7) in step 6) is filtered after purification, is dried to obtain
Sapindoside powder is stand-by；
9) it weighs taro meal 10g to be placed in 350mL flask, addition 150mL distilled water, after isothermal vibration 2h, uses citric acid
It is 6.5 that sodium, which adjusts pH value, addition cellulase and pectase, and the mass ratio of cellulase and pectase is 1.5: 1, is added
Amount is the 1.5% of amount of substrate, liquid-solid ratio 25: 1, ultrasonic extraction 12min；
10) 25U/g α-amylase is first added to extracting solution obtained in step 9), 75 DEG C, is digested under conditions of 6.5 in pH
2h adds the carbohydrase of 25U/g, then carries out enzymatic hydrolysis 2h, and it is stand-by that taro polysaccharide is dried to obtain after high temperature enzyme deactivation；
11) Flos Hibisci Mutabilis extract 0.35g obtained in step 3), step 8) sapindoside extract 10g, step are taken
10) polyoses extract 1.5g in, daily chemical essence 0.2g are added pure water to 150mL, dissolve by heating, room temperature is cooled to after dissolution, use
It is 6.8 to get a kind of green bacteriostatic hand sanitizer that citric acid and sodium citrate, which adjust pH,.
Raw materials used in the present invention, equipment is unless otherwise noted the common raw material, equipment of this field；In the present invention
Method therefor is unless otherwise noted the conventional method of this field.
The above is only presently preferred embodiments of the present invention, is not intended to limit the invention in any way, it is all according to the present invention
Technical spirit any simple modification, change and equivalent transformation to the above embodiments, still fall within the technology of the present invention side
The protection scope of case.
Claims (10)
1. a kind of preparation method of green bacteriostatic hand sanitizer, in terms of g and mL, it is characterised in that the following steps are included:
1) dry Flos Hibisci Mutabilis is taken, petroleum ether is added, decoloration is impregnated in water bath with thermostatic control；
2) mixture of petroleum ether and Flos Hibisci Mutabilis in step 1) is transferred in ball mill, the difference that diameter is 6-15mm is added
Zirconia ball ball milling 20-30min；
3) mixed liquor after ball milling in step 2) is subjected to decompression suction filtration, ethyl alcohol and water will be used to impregnate after filter residue and drying, ultrasound mentions
It takes, obtains Flos Hibisci Mutabilis extract；
4) supernatant after centrifugation in step 3) is concentrated under reduced pressure, it is stand-by obtains Flos Hibisci Mutabilis flavone extract；
5) it by the fresh taro and soapberry pericarp of cleaning peeling, is crushed after forced air drying stand-by；
6) soapberry powder obtained by step 5) is weighed to be placed in container using distilled water as solvent with 1g: 5-10mL solid-to-liquid ratio,
Add cellulase and pectase, constant temperature extracts, and carries out high temperature enzyme deactivation to crude extract；
7) macroporous absorbent resin ethyl alcohol is impregnated and wash to eluate without white opacity generate, then successively with NaOH solution,
HCl solution impregnates, and is washed till neutrality；
8) macroporous resin adsorption of crude extract step 7) obtained by step 6) is filtered after purification, obtains soapberry soap after dry
Glucoside extract, for use；
9) it weighs taro meal 8-12g to be placed in container, addition 150-200mL distilled water, after isothermal vibration 2-3h, uses sodium citrate
PH value is adjusted, cellulase and pectase, ultrasonic extraction 10-15min is added；
10) 20-25U/g α-amylase is first added into extracting solution obtained by step 9), the enzyme under conditions of 70-80 DEG C, pH=6-6.5
Solution, adds the carbohydrase of 20-25U/g, then digested, taro polyoses extract is dried to obtain after high temperature enzyme deactivation, for use；
11) gained Flos Hibisci Mutabilis extract 0.3-0.5g in step 3), sapindoside extract 10-15g obtained by step 8), step are taken
Pure water is added to 100-150mL in rapid 10) gained taro polyoses extract 1-2g, daily chemical essence 0.1-0.3g, and heating for dissolving is molten
Room temperature is cooled to after solution, adjusting pH with citric acid and sodium citrate is 6.5-7 to get green bacteriostatic hand sanitizer.
2. preparation method as described in claim 1, which is characterized in that in step 1), the mass ratio of petroleum ether and Flos Hibisci Mutabilis is
5-7: 1, water bath with thermostatic control temperature is 60-70 DEG C, soaking time 80-120min.
3. preparation method as described in claim 1, which is characterized in that in step 2), the zirconia ball diameter at least 3 of addition
Kind, the zirconia ball heap volume of addition is 300-400mL；Drum's speed of rotation is 300-450r/min, Ball-milling Time 20-30min.
4. preparation method as described in claim 1, which is characterized in that in step 3), concentration of alcohol 65-75wt%, ultrasound
Solid-to-liquid ratio is 1: 20-1: 30 (g/mL) when extraction, and ultrasonic extraction power is 500-600W, extraction time 28-35min.
5. preparation method as described in claim 1, which is characterized in that in step 6), the mass ratio of cellulase and pectase
It is 1: 0.8-1.2, additional amount is the 0.1-0.3% of soapberry powder quality；Constant temperature Extracting temperature is 50-60 DEG C, extraction time
For 2.5-4h, the high temperature enzyme deactivation is boiling water bath 5-10min enzyme deactivation.
6. preparation method as described in claim 1, which is characterized in that in step 7), the concentration of the ethyl alcohol is 90-
The concentration of 95wt%, the NaOH solution and HCl solution is 3-5wt%, soaking time 2-4h.
7. preparation method as described in claim 1, which is characterized in that in step 7), the macroreticular resin is AB-8, CAD-40
Or DM 301.
8. preparation method as described in claim 1, which is characterized in that in step 9), the mass ratio of cellulase and pectase
It is 1.3-1.7: 1, additional amount is the 1.2-1.5% of taro meal quality.
9. preparation method as described in claim 1, which is characterized in that in step 9), adjusting pH with sodium citrate is 6-6.5,
Ultrasonic extraction 10-15min, ultrasonic extraction solid-to-liquid ratio are 1: 20-25g/mL.
10. preparation method as described in claim 1, which is characterized in that in step 10), enzymolysis time 1.5h-2h.
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|---|---|---|---|---|
| CN113476359A (en) * | 2021-06-22 | 2021-10-08 | 广东菁萃生物科技有限公司 | Antibacterial foam hand sanitizer and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107157801A (en) * | 2017-06-13 | 2017-09-15 | 甘肃民族师范学院 | Containing natural Radix Salviae Miltiorrhizae polysaccharide compound antibacterial hand sanitizer and preparation method thereof |
| CN107496273A (en) * | 2017-10-18 | 2017-12-22 | 湖南大三湘油茶生态产业有限公司 | Camellia oleifera fruit mildy wash and preparation method thereof |
| CN108542850A (en) * | 2018-06-21 | 2018-09-18 | 界首市洁净日用品有限公司 | A kind of antibacterial preserving moisture and protecting skin hand cleanser of addition indocalamus leaf flavones-soapberry pericarp total saposins |
-
2019
- 2019-08-21 CN CN201910776050.1A patent/CN110339133A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107157801A (en) * | 2017-06-13 | 2017-09-15 | 甘肃民族师范学院 | Containing natural Radix Salviae Miltiorrhizae polysaccharide compound antibacterial hand sanitizer and preparation method thereof |
| CN107496273A (en) * | 2017-10-18 | 2017-12-22 | 湖南大三湘油茶生态产业有限公司 | Camellia oleifera fruit mildy wash and preparation method thereof |
| CN108542850A (en) * | 2018-06-21 | 2018-09-18 | 界首市洁净日用品有限公司 | A kind of antibacterial preserving moisture and protecting skin hand cleanser of addition indocalamus leaf flavones-soapberry pericarp total saposins |
Non-Patent Citations (2)
| Title |
|---|
| 《中国商品大辞典》编辑委员会: "《中国商品大辞典 中药材分册》", 30 September 1995 * |
| 马丹: "芋头多糖的提取及对烟丝的保润效果", 《农产品加工》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113476359A (en) * | 2021-06-22 | 2021-10-08 | 广东菁萃生物科技有限公司 | Antibacterial foam hand sanitizer and preparation method thereof |
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