CN110338063A - A kind of method, production method and the twisting device of tea tissue cultures of looking for one's roots - Google Patents
A kind of method, production method and the twisting device of tea tissue cultures of looking for one's roots Download PDFInfo
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- CN110338063A CN110338063A CN201910763922.0A CN201910763922A CN110338063A CN 110338063 A CN110338063 A CN 110338063A CN 201910763922 A CN201910763922 A CN 201910763922A CN 110338063 A CN110338063 A CN 110338063A
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- 230000037303 wrinkles Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/12—Rolling or shredding tea leaves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of methods of tea tissue cultures of looking for one's roots, for obtaining band root tea-tree tissue culture seedling；A kind of production method of tea of looking for one's roots is disclosed, makes tea of looking for one's roots for by band root tea-tree tissue culture seedling combination twisting device or the techniques such as rubbing by hand.Look for one's roots tea can stereoscopic multi-layer, continual plantation indoors, ensure that the yield for tea of looking for one's roots；The fresh and alive beauty of the mini plant of tea tree can be sufficiently shown in brew process, had more ornamental value, be obviously improved the quality having tea；And tea of looking for one's roots of the invention, other than the effective component of tender shoots, also with the active constituent, such as stachyose, gossypose etc. of root, having reduces the distinctive health-care efficacy of the tea roots such as blood cholesterol and triglycerides；Tea of looking for one's roots based on production, infusion technology innovation, can enrich the product of existing tea market.
Description
Technical field
The present invention relates to method, production method and the twisting devices of a kind of tea tissue cultures of looking for one's roots, belong to tea field.
Background technique
Tea-leaf beverage --- tea is known as one of big beverage in the world three, in Chinese long-term, cultivation extensively.During tealeaves is derived from
State, tealeaves are used as sacrificial offerings earliest.It but is medicine in Middle West Han Dynasty development from the later period in spring and autumn just by people as vegetable edible
Just develop the thing popularized civil as common beverages for imperial palace high-grade drink and be after the Western Jin Dynasty with, Western Han Dynastry's later period.It was found that earliest
The traces of artificial growth tealeaves have more than 6000 years history in the river snail mountain ruins in Zhejiang Yuyao.
Containing ingredients such as catechin, cholestenone, caffeine, inositol, folic acid, pantothenic acid in tealeaves, it is strong that human body can be promoted
Health.Tealeaves can make drink, containing multiple beneficial ingredient, and have health-care efficacy.Specific manifestation are as follows: can lower cardiovascular and cerebrovascular morbidity and
Mortality risk, play the role of reducing cholesterol and blood pressure, help to reduce the risk for suffering from diabetes, help to prevent and treat presenile it is silly
It stays, has compressive resistance and angst resistance effect, immunity can be improved, sterilizing power can be improved, have weight-reducing effect, in addition, tea can also
Fatigue is eliminated, refreshes oneself, improving eyesight, help digestion, diuresis removing toxic substances, prevent saprodontia, eliminate halitosis.
According to kind and production method and product shape, tea is divided into seven major class: green tea, black tea, oolong tea, yellow tea,
White tea, purple tea, dark green tea.Spring tea, summer tea, autumn tea, winter tea can be divided by gathering and processing according to season.It is found by long-term summary, market
Existing tea can be divided into tealeaves and tea powder two major classes according to the fine mode of its finished product.Wherein, tealeaves is the leaf to tea tree
It is suppressed with bud, the form of leaf and bud can be restored after brewed.But in brew process, be related to the filtering of tealeaves, topple over
And cleaning is all relatively complicated.Later, people had used for reference the drinking method of west coffee, had developed instant tea powder, tea powder
It is the effective component refined by extraction means in tealeaves, belongs to plant extracts, have with the edible natural tealeaves of Chinese tradition
Certain difference.Moreover, production tea powder is higher to raw material and processing technology requirement, belong to high-grade drink, the consumer group is relatively
It is small.It can be seen that the product type of China's tea market need further to enrich, to meet people when having tea to beauty
The high-quality of sight, succinct, health care etc. is pursued.
It was found that the root of fresh tea tree contains the carbohydrates such as stachyose, gossypose, sucrose, glucose, fructose, and containing a small amount of
Polyphenolic substance (flavanols, caffeine etc.).According to pharmacological evaluation, tea root has the work for reducing blood cholesterol and triglycerides
With.The effect of tea root, is main are as follows: heart tonifying diuresis；Promoting blood circulation for regulating menstruation；It is clearing heat and detoxicating.For rheumatic, hypertensive cerebral and lung originality
Heart disease has a certain effect to symptom is improved, and treats coronary heart disease, treatment cardiac arrhythmia " China's book on Chinese herbal medicine ", controls that mouth is rotten, halitosis.It can
See, tea root can be considered completely is dissolved into tealeaves manufacture craft, and then brings the wound in tea processing and infusion technology
Newly.
For this purpose, the tea of looking for one's roots of the advantages that having both ornamental value, plurality of health care functions a kind of can be provided, drinking person can be made simultaneously
The root of the mini plant of tea tree, the fresh and alive beauty of stem, leaf are enjoyed, the quality having tea is promoted, enriches the product type of tea market,
New vitality is brought to tea market.
Summary of the invention
The present invention provides a kind of methods of tea tissue cultures of looking for one's roots, for obtaining band root tea-tree tissue culture seedling；It looks for one's roots tea
Production method, for combining twisting device provided by the present application by band root tea-tree tissue culture seedling or rubbing etc. techniques production by hand
It looks for one's roots tea.
The technical scheme is that a kind of method for tea tissue cultures of looking for one's roots, comprising:
S1, the green young tender stem tip for choosing former tea tree plant, length 1-10cm；
S2, first by green young tender stem tip use on a sterile work bench tap water flowing water rinse 1-2h, under aseptic condition
It is put into 75-95% alcohol and sterilizes 30-60s, aseptic water washing 3-10 times；It is subsequently placed in 0.1% mercuric chloride solution and sterilizes 3-
15min, aseptic water washing 3-10 times；Finally, being divided into the stem-segment with node or stem apex of 0.5-1.5cm, it is inoculated in MS+6-BA0.1-
5.0mg/L+NAA0.01-0.1mg/L carrying out Initial culture in culture medium, sterile in vitro tender shoots is obtained after 15-30 days；
S3, the stem apex or stem-segment with node that sterile in vitro tender shoots is cut to a length of 0.5-1.5cm transfer in culture medium MS+
6-BA0.5-5.0mg/L+NAA0.01-0.1mg/L middle carry out Multiplying culture；After 15-30 days, each stem apex or each belt segment stem
Section all obtains the in vitro Multiple Buds of oneself；Multiple Buds are separated on a sterile work bench and are cut into single plant: the switching of part Multiple Buds
In culture medium MS+6-BA0.5-5.0mg/L+NAA0.01-0.1mg/L, it to be used for shoot proliferation culture；Another part Multiple Buds
It transfers and carries out culture of rootage in culture medium MS+NAA0.01-1.0mg/L；After 15-30 days, the band root tea-tree tissue culture seedling of acquisition,
For the raw material as tea preparation of looking for one's roots, the in vitro Multiple Buds of acquisition are then repeatedly used for above-mentioned shoot proliferation culture and training of taking root
It supports.
The condition of tissue culture room is set as: 15-28 DEG C of room temperature, intensity of illumination is controlled in 1000-4000lx, photoperiod 12-
16h illumination, dark phase 8-12h, light source use LED plant tissue culture white light Special energy-saving lamp.
The temperature of condition of culture controls, and specifically can be regulated to 22-28 DEG C of daytime according to the temperature variations in each place of production,
15-20 DEG C of evening.
The MS minimal medium for Initial culture, Multiplying culture, shoot proliferation culture can also be that B5 is trained substantially
Support base；MS minimal medium for culture of rootage can also be 1/2MS culture medium.
It is described for Initial culture, Multiplying culture, shoot proliferation culture plant hormone 6-BA and NAA；Wherein, 6-BA can
To be basic element of cell division 6-BA, GA, BAP, ZT, KT, 2,4-D one of or several, NAA can be auxin NAA, IAA,
IBA is one of or several.
A kind of production method for tea of looking for one's roots, collection belt root tea-tree tissue culture seedling by rubbing or twisting device by hand, and combine
The manufacture craft of traditional different cultivars tea, the corresponding tea of looking for one's roots of production.
Twisting device in a kind of production method that realizing tea of looking for one's roots, by rubbing plate lift adjustment nut 1, hand-operated lifting surely
Mechanism 2 rubs plate 3 surely, moves and rub layer 4, outfeed platforms 5, foot prop 6, rub layer 7 surely, move and rub a layer subiculum platform 8, move and rub
Layer pop-up platform 9, motor 10, belt 11, shaft 12, pan feeding platform 13 and dynamic layer pop-up platform support 14 of rubbing form；It is whole
A twisting device is supported on work top by foot prop 6, is rubbed layer subiculum platform 8 and is located on foot prop 6, each foot prop 6
Outside be connected separately with it is dynamic rubs a layer pop-up platform support 14, be used to support it is dynamic rub a layer pop-up platform 9, it is dynamic that rub layer pop-up flat
It is fixed with manual lifting mechanism 2 on platform 9 and is connect with plate 3 is rubbed surely, rubs layer 7 surely and is adhered closely to rub 3 bottom of plate surely, it is fixed
Rub the lifting that layer 7 is rubbed in plate 3 and its bottom surely, by 2 top of manual lifting mechanism determine to rub plate lift adjustment nut 1 into
Row is adjusted, amplitude 2-10mm；It moves to rub layer subiculum platform 8 and move and rub between layer pop-up platform 9,10 He of motor is installed
Shaft 12 is simultaneously connected by belt 11, and the rotation of shaft 12 drives the dynamic rotation for rubbing layer 4, is rubbed plate 3 surely positioned at dynamic and is rubbed layer
The top of 9 side of pop-up platform, dynamic dynamic 4 platform of layer of rubbing for rubbing layer 9 other side of pop-up platform is as pan feeding platform 13.
The dynamic layer 4 of rubbing is annular rubber layer, Zhou Changwei 2-10m, passively rubbed respectively when mobile a layer subiculum platform 8,
Dynamic layer pop-up platform 9 of rubbing supports.
Surely the plate 3 of rubbing is steel, and the length is 0.5-4.5m, width 0.5-1.5m, height 0.02-0.1m, tea
Leaf entrance side is designed at rounded corner；Surely rub layer 7 be made of rubber or plank and rub surely one side that layer 7 is contacted with tealeaves at
Corrugated design.
The length of the pan feeding platform 13 is 0.5-4m, width 0.5-1.5m.
The beneficial effects of the present invention are:
1) compared with the spring harvesting for traditionally planting tealeaves, tea of looking for one's roots of the invention can produce throughout the year indoors, show
Work improves the freshness in summer, autumn and winter tealeaves.Meanwhile look for one's roots tea can stereoscopic multi-layer, continual plantation indoors,
It ensure that the yield for tea of looking for one's roots.
2) traditional ground, which plants disease for tea plant, more than 100, common are more than 30 kinds, such as sprays insecticide, then may bring
Certain pesticide residue, if do not sprayed insecticide, then it is difficult to ensure that the high-quality of tealeaves.Tea of looking for one's roots of the invention is in tissue cultures mistake
Cheng Zhong, absolutely not any pest and disease damage occur, and are without the use of any pesticide, and zero agriculture is residual, has stable high-quality.
3) during traditional tealeaves is brewed, stem, the leaf portion point of tealeaves tender shoots are only shown, and tea of looking for one's roots of the invention has
Root, stem, the leaf of tea tree seedling can sufficiently show the fresh and alive beauty of the mini plant of tea tree in brew process, have more ornamental value, show
Work improves the quality having tea, and is more able to satisfy the curiosity that the crowds such as the children of non-tea growing areas understand tea tree entirety plant.
4) traditional tealeaves, including tea powder, in brewed tea-drinking, the only effective ingredient of tea tree tender shoots, and it is of the invention
Tea of looking for one's roots, also with the active constituent, such as stachyose, gossypose etc. of root, there is reduction blood other than the effective component of tender shoots
The distinctive health-care efficacy of the tea roots such as middle cholesterol and triglycerides.
5) tea of looking for one's roots based on production, infusion technology innovation, can enrich product (tealeaves, tea powder) class of existing tea market
Type brings new vitality to tea market.
6) " look for one's roots and do not forget the past suffering ", with root health tea --- the research and development and release for tea of looking for one's roots, can power-assisted in " stay true to the mission, jail
The advocation of note mission " themes of the times, has very wide application prospect.
Detailed description of the invention
Fig. 1 is the production technological process of tea of looking for one's roots；
Fig. 2 is the special twisting device schematic diagram of tea of looking for one's roots；
Fig. 3 is the infusion conditions figure of tea of looking for one's roots；
In figure: 1- rubs plate lift adjustment knob surely；2- manual lifting mechanism；3- rubs plate surely；4- is dynamic to rub layer；5- goes out
Expect platform；6- foot prop；7- rubs layer surely；8- is dynamic to rub a layer subiculum platform；9- is dynamic to rub a layer pop-up platform；10- motor；11-
Belt；12- shaft；13- pan feeding platform；14- is dynamic to rub a layer pop-up platform support.
Specific embodiment
Embodiment 1: a method of tea tissue cultures of looking for one's roots, comprising:
S1, the green young tender stem tip for choosing former tea tree plant, length 1-10cm；Former Resources of Tea Plant includes Camellia
(Camellia) plant can be used for all species and its kind of drink tea production, specifically include following strain: five precious tea systems: wide
Western tea, Guangnan tea, five Room tea, dredges tooth tea at big luxuriant tea.Five column tea systems: C.crassicolumna Chang, five column tea, old dark green tea, Dali tea, Yunnan Burma tea,
Garden base tea, wrinkle leaf tea, Maguan tea, Hani tea, multiple-petal tea.Bald room tea system: Menla tea, costal tea, intends thin calyx tea, vacation at Dehong tea
Prominent room tea, Rongjiang tea, purple fruit tea, more arteries and veins tea.Tea system: tea, raw white tea (mutation), Pu'er tea, more calyx tea, is intended at herb mixtures tea (mutation)
Thin calyx tea, Yuanjiang River tea, Gao Shucha.And group's kind: brave library daye tea, Menhai daye tea, (the above are national levels for Fengqing daye tea
Breeding), the glutinous tea of Yuanjiang River, the big white tea of seedling tower, town source stirrup tea, Lvchun County's Ma jade tea, vast and boundless water daye tea, Iceland's daye tea, embankment is white
Gross tea, Cloud Dragon Hill daye tea, scape paddy daye tea roll into a ball field daye tea, nation's east daye tea, official stockaded village tea, big factory's tea, billows deep blue daye tea
Deng and Clonal Cultivar: select the offspring of big-leaf species in yunnan with vegetative manner, mainly there is that cloud is No. 43 anti-, it is white to come into leaves
In the least；Cloud is No. 10 anti-, cloud is No. 14 anti-；It is Yun Mei, Yun Gui, short rich etc.；Cloud selects No. 9, cloud anti-No. 37.And the hybridization in other Camellias
Strain and kind etc..
S2, first by green young tender stem tip use on a sterile work bench tap water flowing water rinse 1-2h, under aseptic condition
It is put into 75-95% alcohol and sterilizes 30-60s, aseptic water washing 3-10 times；It is subsequently placed in 0.1% mercuric chloride solution and sterilizes 3-
15min, aseptic water washing 3-10 times；Finally, being divided into the stem-segment with node or stem apex of 0.5-1.5cm, it is inoculated in MS+6-BA0.1-
5.0mg/L+NAA0.01-0.1mg/L carrying out Initial culture in culture medium, sterile in vitro tender shoots is obtained after 15-30 days；
S3, the stem apex or stem-segment with node that sterile in vitro tender shoots is cut to a length of 0.5-1.5cm transfer in culture medium MS+
6-BA0.5-5.0mg/L+NAA0.01-0.1mg/L middle carry out Multiplying culture；After 15-30 days, each stem apex or each belt segment stem
Section all obtains the in vitro Multiple Buds of oneself；Multiple Buds are separated on a sterile work bench and are cut into single plant: the switching of part Multiple Buds
In culture medium MS+6-BA0.5-5.0mg/L+NAA0.01-0.1mg/L, it to be used for shoot proliferation culture；Another part Multiple Buds
It transfers and carries out culture of rootage in culture medium MS+NAA0.01-1.0mg/L；After 15-30 days, the band root tea-tree tissue culture seedling of acquisition,
For the raw material as tea preparation of looking for one's roots, the in vitro Multiple Buds of acquisition are then repeatedly used for above-mentioned shoot proliferation culture and training of taking root
It supports.
It is possible to further which the pH value of the culture medium is arranged, with specific reference to the pH value situation of each Producing Area Soil, it is adjusted to
4.5-5.5。
It is possible to further which the condition of tissue culture room to be set as: 15-28 DEG C of room temperature, intensity of illumination is controlled in 1000-
4000lx, the illumination of photoperiod 12-16h, dark phase 8-12h, light source use LED plant tissue culture white light Special energy-saving lamp.
Controlled it is possible to further the temperature to condition of culture, specifically can according to the temperature variations in each place of production,
It is regulated to 25-26 DEG C of daytime, 15-20 DEG C of evening.
It is possible to further be arranged it is described for Initial culture, Multiplying culture, shoot proliferation culture MS minimal medium
It can also be B5 minimal medium；MS minimal medium for culture of rootage can also be 1/2MS culture medium.
It is possible to further be arranged it is described for Initial culture, Multiplying culture, shoot proliferation culture plant hormone 6-BA
And NAA；Wherein, it is one of or several to can be basic element of cell division 6-BA, GA, BAP, ZT, KT, 2,4-D by 6-BA, and NAA can be with
It is auxin NAA, IAA, IBA one of or several.
A kind of production method for tea of looking for one's roots, collection belt root tea-tree tissue culture seedling by rubbing or twisting device by hand, and combine
The manufacture craft of traditional different cultivars tea, the corresponding tea of looking for one's roots of production.The band root tea-tree tissue culture seedling, can be rushed by drinking water
Wash off the culture medium that root is sticked；By rubbing or twisting device by hand, in combination with conventional green tea, black tea, oolong tea, white
Tea, yellow tea, purple tea or dark green tea manufacture craft, fry, dry, shine or steam etc., it is fabricated to green tea of looking for one's roots accordingly, black tea of looking for one's roots, looks for one's roots
Oolong tea, white tea of looking for one's roots, yellow tea of looking for one's roots, look for one's roots purple tea or dark green tea of looking for one's roots.Mainly include green tea: mee tea, green tea, delicate fried green,
Generous, Pilochun (a green tea), yuhua tea, sweet dew, pine needle；Common baking, delicate baking etc.；River blueness, Yunnan blueness, Shan blueness etc.；Steaming green tea point
Are as follows: it simmers tea, beautiful dew etc..Souchong: Lapsang souchong, cigarette microspecies；Congou tea: river red (Jin Ganlu, red sweet dew etc.), keemun,
Yunnan black tea, Fujian red (golden fine horse eyebrow etc.) etc.；Broken black tea: leaf tea, fannings, tablet tea, dust tea.Oolong tea: the north of Fujian Province oolong (Wuyi cliff tea ---
Clovershrub, narcissus, cortex cinnamomi, waist half a day, odd orchid, Eight Immortals etc., the also tea of the real estates such as some Jian'ou Jianyangs, such as short-foot oolong)；Fujian
(Iron Guanyin, odd orchid, narcissus, gold osmanthus etc., narcissus and Qi Lan here is primarily referred to as the difference on main ground to southern oolong, same to grow tea
The tea that ground produces in the different places of production)；Guangdong oolong (phoenix list fir, phoenix narcissus, the single fir of ridge head etc.)；Taiwan oolong (freezes top crow
Dragon, packet kind etc., what that of the first two years prevalence was east beauty is also this) Alishan high mountainous tea (Alishan QINGXINWULONG tea,
Alishan superfine product gold tawny daylily tea etc.).White tea: white young tea leaves is primarily referred to as acupuncture needle etc.；Bai Yecha is primarily referred to as white peony, tribute eyebrow etc..
Yellow tea: yellow bud, Junshan Silver Needle Tea etc. yellow young tea leaves: are pushed up including covering；Huang little Cha: including northern port high-quality green tea, Weishanmajian, Wenzhou yellow stuff etc.；
Huang great Cha: including Huoshan Huang great Cha, Guangdong great Ye blueness etc..Purple tea: such as purple beautiful tea.Dark green tea: Hunan dark green tea (Anhua dark green tea etc.)；
Hubei green tea (Pu boundary always green tea etc.) always；Sichuan brick tea (South Road brick tea and West Road brick tea etc.)；Yunnan osmanthus dark green tea (six fort tea etc.)；Shan
Western dark green tea (river rising in Ningxia and flowing into central Shaanxi Weihe Fu tea etc.).And some other local varieties.The craft is rubbed or twisting device, specifically makes band root tea tree
Tissue-cultured seedling rub into strips, spiral, curly, imperial trip shape etc., and keep the high integrity of root, stem and leaf, and the device of the application
Manpower is saved in alternative craft again.
Twisting device in a kind of production method that realizing tea of looking for one's roots, by rubbing plate lift adjustment nut 1, hand-operated lifting surely
Mechanism 2 rubs plate 3 surely, moves and rub layer 4, outfeed platforms 5, foot prop 6, rub layer 7 surely, move and rub a layer subiculum platform 8, move and rub
Layer pop-up platform 9, motor 10, belt 11, shaft 12, pan feeding platform 13 and dynamic layer pop-up platform support 14 of rubbing form；It is whole
A twisting device is supported on work top by foot prop 6, is rubbed layer subiculum platform 8 and is located on foot prop 6, each foot prop 6
Outside be connected separately with it is dynamic rubs a layer pop-up platform support 14, be used to support it is dynamic rub a layer pop-up platform 9, it is dynamic that rub layer pop-up flat
It is fixed with manual lifting mechanism 2 on platform 9 and is connect with plate 3 is rubbed surely, rubs layer 7 surely and is adhered closely to rub 3 bottom of plate surely, it is fixed
Rub the lifting that layer 7 is rubbed in plate 3 and its bottom surely, by 2 top of manual lifting mechanism determine to rub plate lift adjustment nut 1 into
Row is adjusted, amplitude 2-10mm；It moves to rub layer subiculum platform 8 and move and rub between layer pop-up platform 9,10 He of motor is installed
Shaft 12 is simultaneously connected by belt 11, and the rotation of shaft 12 drives the dynamic rotation for rubbing layer 4, is rubbed plate 3 surely positioned at dynamic and is rubbed layer
The top of 9 side of pop-up platform, dynamic dynamic 4 platform of layer of rubbing for rubbing layer 9 other side of pop-up platform is as pan feeding platform 13.It rubs
When operation, power on first, and adjusting motor 10 to drive dynamic 4 movement velocity of layer of rubbing by adjusting knob is 0.1-
2.0cm/s, while being adjusted by rubbing plate lift adjustment nut 1 surely and rubbing determining to rub layer 7 and dynamic rubbing layer 4 for 3 bottom of plate surely
Distance be 1-10mm, then will be sequentially placed with root tea-tree tissue culture seedling in the dynamic pan feeding platform 13 for rubbing 4 side of layer, by fixed
Layer 7 and the dynamic relative motion for rubbing layer 4 are rubbed, can then be rubbed into root tea-tree tissue culture seedling different degrees of linear, curly
Deng, finally removed naturally from outfeed platforms 5, and combine the manufacture craft of traditional different cultivars tea, become green tea of looking for one's roots, black tea,
The primary product such as white tea, yellow tea, oolong tea, purple tea, dark green tea.Acquisition look for one's roots green tea, black tea, white tea, yellow tea, oolong tea, purple tea,
It after the primary product such as dark green tea, screened, be classified, specifically sort out the primary product of root or leaf abscission, badly broken, so
After filter out root and the intact tea of looking for one's roots of blade as Grade A, remaining tea of looking for one's roots is as goods of inferior quality.Finally, to looking for one's roots
Tea is packed, 1-10/bag of quantity, and every bag is steeped as one to get to tea finished product of looking for one's roots, and is used for outer packing.
Determine to rub plate lift adjustment nut 1 it is possible to further be arranged the manual lifting mechanism 2 and upper part, also
Electric up-down equipment substitution can be used.
It is annular rubber layer it is possible to further which the dynamic layer 4 of rubbing is arranged, Zhou Changwei 2-10m, it is passive to distinguish when mobile
Rub a layer subiculum platform 8, dynamic layer pop-up platform 9 of rubbing supports.
It is steel it is possible to further which the plate 3 of rubbing surely is arranged, the length is 0.5-4.5m, width 0.5-1.5m,
Height 0.02-0.1m, tea inlet side are designed at rounded corner；Surely rub layer 7 be made of rubber or plank and rub surely layer 7 with
The corrugated design of one side of tealeaves contact.
It is 0.5-4m, width 0.5-1.5m it is possible to further which the length of the pan feeding platform 13 is arranged.
Specifically give following example:
First example: most northern tea place being distributed from China in March, 2019 --- the individual tea place of Laoshan acquires green
Young tender stem tip 56, length 1-10cm (such as 1cm, 2cm, 3.5cm, 10cm) uses tap water on a sterile work bench first
Flowing water rinses 1h.Through 75% alcohol disinfecting 30s under aseptic condition, 0.1% mercuric chloride sterilizes 6min, and aseptic water washing 5 times.Then
It is divided into the stem-segment with node or stem apex of 1.0-1.5cm (such as 1.0cm, 1.1cm, 1.5cm), is inoculated in MS+6-BA2.0mg/L
In+NAA0.1mg/L culture medium, sterile in vitro tender shoots is obtained within 30 days.
Further, sterile in vitro tender shoots being cut a length of 1.0-1.5cm (such as 1.0cm, 1.1cm, 1.5cm)
Stem apex or stem-segment with node, switching carry out Multiplying culture in culture medium MS+6-BA3.0mg/L+IBA0.1mg/L.It 15 days, obtains
In vitro Multiple Buds (average each 3.2 buds).Then, Multiple Buds are separated on a sterile work bench and is cut into single plant, will owned
Multiple Buds are transferred in MS+6-BA3.0mg/L+IBA0.1mg/L, are continued Multiplying culture 2 times, in June, 2019, and tender shoots number is obtained
It is 489 total.Then, 189 Multiple Buds switchings are continued into Multiplying culture in MS+6-BA3.0mg/L+NAA0.1mg/L, it will
Remaining 300 buds switching carries out culture of rootage in culture medium MS+IBA0.1mg/L.15 days, root long 3-5cm, radical was average
5.6, the tea of looking for one's roots with root tissue-cultured seedling for the later period of acquisition prepares raw material.
Further, the pH value of culture medium is adjusted to 4.5 with specific reference to the soil pH value situation in Pu'er.It is strong to cultivate illumination
Degree control uses LED plant tissue culture white light Special energy-saving lamp in 2200lx, the illumination of photoperiod 14h, dark phase 10h, light source.Illumination period
Temperature is controlled at 22-25 DEG C, and dark phase temperature control has Qingdao characteristics quality and flavor at 15-18 DEG C and then by cultivating to obtain
Tea of looking for one's roots.
Further, 300 tea tree aseptic seedlings with root are concentrated, the culture that root is sticked is rinsed out by drinking water
Base.
Further, by cleaned band root tea-tree tissue culture seedling marshalling, baking oven or steam box are placed in tea tree seedling
It finishes, then band root seedling is rubbed by manual or special equipment of rubbing, tea tree seedling is made to be rolled into strip,
And keep the high integrity of root, stem and leaf.
Specifically, be adjusted to suitably move by motor 10 and rub 4 movement velocity 0.1cm/s of layer when twisting device operation,
Being adjusted by rubbing plate adjusting knob 1 surely and rubbing determine the to rub layer 7 and dynamic rub of 3 bottom of plate surely is 1mm at a distance from layer 4, then
It will be sequentially placed with root tea-tree tissue culture seedling in the dynamic pan feeding platform 13 for rubbing 4 side of layer, after tea-tree tissue culture seedling rubs into threadiness,
It is removed from outfeed platforms 5.
Further, fried dry, dry and be dried by way of drying, acquisition is looked for one's roots green tea head product, and to it
It screened, be classified, specifically sorted out the primary product of root or leaf abscission, badly broken, then filter out root and blade
Intact tea of looking for one's roots is as Grade A, and remaining tea of looking for one's roots is as goods of inferior quality.
Finally, pack to green tea of looking for one's roots, 3/bag of quantity, every bag as a bubble to get to tea finished product of looking for one's roots.
As shown in Figure 3 (dark brown in green), root, Ye Zhankai have preferable ornamental value to effect after green tea of looking for one's roots is brewed,
It has been obviously improved the quality having tea.
Second example: Jiangsu looks for one's roots the production of Pilochun (a green tea)
It is collected in individual tea place Green young tender stem tip 100 of Jiangsu, length 1-6cm, acquisition in March, 2019
Afterwards by being delivered to laboratory fastly with fresh mode along rich express delivery.
The Initial culture rinses 2h with tap water flowing water on a sterile work bench first, then passes through under aseptic condition
75% alcohol disinfecting 60s, 0.1% mercuric chloride sterilize 15min, aseptic water washing 10 times.It is then split into 0.5.0-1.2cm (such as
0.5cm, 1.0cm, 1.1cm, 1.2cm etc.) stem-segment with node or stem apex, be inoculated in MS+6-BA3.0mg/L+NAA0.1mg/L
In culture medium, sterile in vitro tender shoots is obtained within 15 days.
Further, sterile in vitro tender shoots is cut into a length of 0.5-1.2cm (such as 0.5cm, 1.0cm, 1.1cm, 1.2cm
Etc.) stem apex or stem-segment with node, switching Multiplying culture is carried out in culture medium MS+KT3.0mg/L+IAA0.1mg/L.30 days,
Obtain in vitro Multiple Buds (average each 3.0 buds).Then, Multiple Buds are separated on a sterile work bench and is cut into single plant, it will
All Multiple Buds switchings continue Multiplying culture 2 times, in June, 2019 in MS+KT3.0mg/L+IAA0.1mg/L, obtain tender shoots
Number is 475 total.Then, 175 Multiple Buds switchings are continued into Multiplying culture in MS+KT3.0mg/L+IAA0.1mg/L, it will
Remaining 300 buds switching carries out culture of rootage in culture medium 1/2MS+IAA0.1mg/L.30 days, root long 3-5cm, radical was flat
Equal 4.6, the Pilochun (a green tea) of looking for one's roots with root tissue-cultured seedling for the later period of acquisition prepares raw material.
All kinds of nutrients of the MS culture medium are formulated situation according to the soil nutrient in each Jiangsu place of production, specifically increase
Trace elements of selenium 0.20mg/L, pH value are adjusted to 5.5, and obtaining by culture, there is the selenium-rich of Yixing characteristic quality and flavor to look for one's roots
Green tea, the concentration of selenium reach 0.32mg/kg fresh weight.
The condition of the tissue cultures is set as: 25-26 DEG C of room temperature, intensity of illumination is controlled in 1000lx, photoperiod 13h light
According to dark phase 11h, light source uses LED plant tissue culture white light Special energy-saving lamp.
Further, 300 tea tree aseptic seedlings with root are concentrated, the culture that root is sticked is rinsed out by drinking water
Base.Then by cleaned band root tea-tree tissue culture seedling marshalling, baking oven or steam box is placed in and is finished to tea tree seedling, so
Rub equipment by manual or special afterwards band root seedling rubbed, tea tree seedling is made to be rolled into strip, and keep root,
The high integrity of stem, leaf.
Further, after removal threadiness rubs material, manual rubbing is carried out, or utilize twisting device rubbing.
4 movement velocity 1.5cm/s of layer is rubbed specifically, being adjusted to suitably move by motor 10, by rubbing plate tune surely
The layer 7 of determining to rub that 3 bottom of plate is rubbed in the section adjusting of knob 1 surely is rubbed with dynamic for 8mm at a distance from layer 4, then will be with root tea-tree tissue culture
Seedling is sequentially placed in the dynamic pan feeding platform 13 for rubbing 4 side of layer, is rubbed after material is twisted into group after threadiness, is removed from outfeed platforms 5.
Further, it is dried by way of drying, obtains Pilochun (a green tea) head product of looking for one's roots, and it is screened,
Classification, specifically sorts out the primary product of root or leaf abscission, badly broken, then filters out root and blade is intact
Pilochun (a green tea) look for one's roots as Grade A, remaining Pilochun (a green tea) of looking for one's roots is as goods of inferior quality.
Finally, pack to green Pilochun (a green tea) of looking for one's roots, 3/bag of quantity, every bag as a bubble to get to Pilochun (a green tea) of looking for one's roots
Finished product.
Third example: Pu'er tea makes black tea of looking for one's roots
Green young tender stem tip 50, length 1-5cm of garden Pu'er tea are looked into March, 2019, acquisition Kunming botanical garden, first
1h is rinsed with tap water flowing water on a sterile work bench.Through 95% alcohol disinfecting 45s, the disinfection of 0.1% mercuric chloride under aseptic condition
3min, aseptic water washing 5 times.It is then split into the stem-segment with node or stem apex of 1.0-1.5cm, is inoculated in MS+6-BA1.0mg/L+
In NAA0.1mg/L culture medium, sterile in vitro tender shoots is obtained within 20 days.
Further, sterile in vitro tender shoots is cut to the stem apex or stem-segment with node of a length of 1.0-1.5cm, is transferred in culture
Multiplying culture is carried out in base MS+6-BA3.0mg/L+NAA0.1mg/L.30 days, it is (each 3.5 average to obtain in vitro Multiple Buds
Bud).Then, Multiple Buds are separated on a sterile work bench and is cut into single plant, all Multiple Buds are transferred in MS+6-BA3.0mg/
In L+NAA0.1mg/L, continue Multiplying culture 2 times, in June, 2019, it is 512 total to obtain tender shoots number.Then, 112 are grown thickly
Bud is transferred in MS+6-BA3.0mg/L+NAA0.1mg/L, and Multiplying culture is continued, and remaining 400 buds are transferred in culture medium
Culture of rootage is carried out in MS+NAA0.1mg/L.25 days, root long 3-5cm, radical was 6.5 average, and the band root tissue-cultured seedling of acquisition is used for
The tea of looking for one's roots in later period prepares raw material.
The pH value of the culture medium is adjusted to 4.8 with specific reference to the soil pH value situation in Pu'er, and then obtains by culture
There must be the tea of looking for one's roots of each place of production characteristic quality and flavor.
The condition of the tissue cultures is set as: 25-28 DEG C of room temperature, intensity of illumination is controlled in 2500lx, 12 illumination of photoperiod,
Dark phase 12h, light source use LED plant tissue culture white light Special energy-saving lamp.
Further, 400 tea tree aseptic seedlings with root are concentrated, the culture that root is sticked is rinsed out by drinking water
Base.
Further, by cleaned band root tea-tree tissue culture seedling proper alignment in bamboo plaque, heating deterioration and outdoor indoors
Daylight withers；Then band root seedling is rubbed by manual or special equipment of rubbing, tea tree seedling is made to be rolled into strip,
And keep the high integrity of root, stem, leaf.
Specifically, be adjusted to suitably move by motor 10 and rub 4 movement velocity 2.0cm/s of layer when twisting device operation,
Being adjusted by rubbing plate adjusting knob 1 surely and rubbing determine the to rub layer 7 and dynamic rub of 3 bottom of plate surely is 10mm at a distance from layer 4, then
It will be sequentially placed with root tea-tree tissue culture seedling in the dynamic pan feeding platform 13 for rubbing 4 side of layer, after tea-tree tissue culture seedling rubs into threadiness,
It is removed from outfeed platforms 5.
Further, by the tea embryo rubbed in basket, after slightly compressing, the dipped fermentation cloth of warm water is covered, is sent out
Ferment 5 hours；It is baked and banked up with earth when vein is in bronzing, at 80 degree, the time controlled at 5 hours temperature；Multiple roasting is finally carried out, makes to seek
The water content of root tea is no more than 8% to get black tea primary product of looking for one's roots.
Further, black tea primary product of looking for one's roots screened, be classified, specifically sorted out root or leaf abscission, break
Serious primary product are damaged, then filter out root and the intact tea of looking for one's roots of blade as Grade A, remaining tea work of looking for one's roots
For goods of inferior quality.
Finally, pack to black tea of looking for one's roots, 3/bag of quantity, every bag as a bubble to get to tea finished product of looking for one's roots.
As shown in Figure 3 (dark brown to take on a red color), root, Ye Zhankai have preferable ornamental value to effect after black tea of looking for one's roots is brewed,
It has been obviously improved the quality having tea.
Above-mentioned to present three groups of experimental datas, in addition to value given herein above, all data with range in the application can be with
Any selection is carried out in the range of setting.
MS culture medium the preparation method is as follows:
A: a great number of elements (mother liquor I):
NH4NO3, 33000mg/L；
KNO3, 38000mg/L；
CaCl2·2H2O, 8800mg/L；
MgSO4·7H2O, 7400mg/L；
KH2PO4, 3400mg/L.
B: microelement (mother liquor II):
KI, 166mg/L；
H3BO3, 1240mg/L；
MnSO4·4H2O, 4460mg/L；
ZnSO4·7H2O, 1720mg/L；
Na2MoO4·2H2O, 50mg/L；
CuSO4·5H2O, 5mg/L；
CoCl2·6H2O, 5mg/L.
C: molysite (mother liquor III):
FeSO4·7H2O, 5560mg/L；
Na2-EDTA·2H2O, 7460mg/L.
D: IV A of organic principle (mother liquor IV):
20 000mg/L of inositol；
IV B niacin 100mg/L；
Puridoxine hydrochloride (vitamin B6) 100mg/L；
Thiamine hydrochloride (vitamin B1) 20mg/L；
Glycine 400mg/L.
The dosage of the above various nutritional ingredients, other than mother liquor I is 20 times of concentrates, remaining is 200 times of concentrates.
Above-mentioned several mother liquors will individually be made into the stock solution of 1L.Wherein:
The preparation method of mother liquor I, mother liquor II and mother liquor IV is: several ingredients in every kind of mother liquor weigh respectively, specific:
Mother liquor I:
NH4NO3, 33000mg*20=0.66g；
KNO3, 38000mg*20=0.76g；
CaCl2·2H2O, 8800mg*20=0.176g；
MgSO4·7H2O, 7400mg*20=0.148g；
KH2PO4, 3400mg*20=0.068g.
Mother liquor II:
KI, 166mg*200=0.0332g；
H3BO3, 1240mg*200=0.248g；
MnSO4·4H2O, 4460mg*200=0.892g；
ZnSO4·7H2O, 1720mg*200=0.344g；
Na2MoO4·2H2O, 50mg*200=0.01g；
CuSO4·5H2O, 5mg*200=0.001g；
CoCl2·6H2O, 5mg*200=0.001g.
Mother liquor IV:
Inositol 20000mg*200=4g；
IV B niacin 100mg*200=0.02g；
Puridoxine hydrochloride (vitamin B6) 100mg*200=0.02g；
Thiamine hydrochloride (vitamin B1) 20mg*200=0.004g；
Glycine 400mg*200=0.08g.
Further, after mother liquor I, mother liquor II and mother liquor IV weigh, respectively plus distilled water thoroughly dissolves, and then again will
Each ingredient difference of several mother liquors is miscible, and finally plus distilled water constant volume is to 1L.
The preparation method of mother liquor III is: the FeSO that will be weighed up4·7H2O (5560*200mg) and Na2-EDTA·2H2O
(7460*200mg) is put into respectively in 450mL distilled water, and the side Bian Jiare, which is stirred continuously, dissolves them, then mixes two kinds of solution
It closes, and pH is adjusted to 5.5, last constant volume to 1L is stored in Brown Glass Brown glass bottles and jars only.After various mother liquors have been matched, vial is used respectively
Storage, and it is labelled, indicate mother liquor number, prepare multiple, date etc., it is stored in the refrigerating chamber (4-10 DEG C of temperature) of refrigerator
In.
When preparing culture medium, take out required dosage respectively from various mother liquors with graduated cylinder or pipette: mother liquor I is
50mL, each 5mL of mother liquor II, III, IV.When dissolving agar, agar 9g, sucrose 30g are weighed respectively with thick balance, is put into 1000mL's
In enamel graduate, distilled water 750mL, electricity consumption stove heating are added, Bian Jiare is stirred on side with glass bar, until liquid is translucent
Shape.Then the mixed-culture medium prepared is added in the agar boiled again, finally plus distilled water constant volume is to 1000mL, and stirring is
It is even.
The plant hormone 6-BA and NAA for Multiplying culture, wherein 6-BA can also be basic element of cell division 6-BA,
GA, BAP, ZT, KT, 2,4-D etc. are one of or several, and NAA can also be that auxin NAA, IAA, IBA etc. are one of or several
Kind.
All kinds of nutrients of the MS culture medium, can also according to the nutrient of each Producing Area Soil be formulated situation, specifically increase or compared with
Few corresponding a great number of elements, microelement, sugar content.Such as selenium, calcium, iron, zinc, and then obtain by culture with each place of production
Characteristic quality and the selenium-rich of flavor are looked for one's roots tea, and the concentration of selenium reaches 0.25mg/kg~4.0mg/kg fresh weight.
PH value is adjusted, the NaOH solution for being 1mol/L with the amount concentration of dropper draws substance instills the culture medium dissolved dropwise
In, it is stirred in drop, and survey the pH of culture medium with pH test paper or electronics pH meter at any time.With specific reference to the pH value feelings of each Producing Area Soil
Condition is adjusted to 4.5-5.5, and then obtains the tea of looking for one's roots with each place of production characteristic quality and flavor by culture.
The packing of culture medium, the culture medium dissolved should dispense while hot.When packing, first culture medium is poured into beaker, so
The culture medium in beaker is poured into culture bottle (220-380mL) afterwards.It is careful not to that culture medium is allowed to be stained on bottleneck and bottle wall.Often
1000mL culture medium can dispense 25-30 bottles, make the culture medium thickness 0.5-1.5cm in culture bottle.Culture medium packing finishes
Afterwards, bottleneck is covered in time.
In addition, the tea for different cultivars provides the following concrete example in conjunction with manufacture craft:
It is glutinous to rinse out root by drinking water for the preparation method of the tea of looking for one's roots, first the tea tree aseptic seedling of collection belt root
Attached culture medium.Then by special twisting device, and traditional green tea, black tea, white tea, yellow tea, oolong tea, purple are combined
The manufacture crafts such as tea, dark green tea, the corresponding green tea of looking for one's roots of production, white tea of looking for one's roots, yellow tea of looking for one's roots, oolong tea of looking for one's roots, are sought black tea of looking for one's roots
Root purple tea, dark green tea of looking for one's roots etc..
Specifically, the preparation of the green tea of looking for one's roots, first finishes to tea tree seedling by baking oven or steam box, then
Band root seedling is rubbed by manual or special equipment of rubbing, tea tree seedling is made to be rolled into strip or spiral (such as green spiral shell
Spring), and keep the high integrity of root, stem and leaf.Finally, fried dry, drying and being dried by way of drying, looked for one's roots
Green tea head product.
The preparation of the black tea of looking for one's roots etc., first by the band root tea-tree tissue culture seedling proper alignment of collection in bamboo plaque, indoors
Heating deterioration and outdoor daylight are withered；Then band root seedling is rubbed by manual or special equipment of rubbing, makes tea
Tree seedling is rolled into strip；By the tea embryo rubbed in basket, after slightly compressing, the dipped fermentation cloth of warm water is covered, is fermented
4-8 hours；It is baked and banked up with earth when vein is in bronzing, temperature is controlled in 70-90 degree, time at 4-8 hours；It is answered before packaging
The water content of roasting, the tea that makes to look for one's roots is no more than 8%.
The preparation of the white tea of looking for one's roots, first by the band root tea-tree tissue culture seedling proper alignment of collection in bamboo plaque, according to weather
Situation allows band root tealeaves to carry out natural air drying and withers；The tealeaves proper alignment withered in dryer, adjustment time and temperature
Degree just bake and makees；It is come out after just drying, spreading for cooling 10-20 minutes, picks and pick root, leaf falls off or the tealeaves that damages；Packaging is advanced
The water content of the multiple roasting of row, the tea that makes to look for one's roots is no more than 8%.
The preparation of the yellow tea of looking for one's roots first finishes to tea tree seedling by baking oven or steam box, then passes through hand
Work or special equipment of rubbing rub band root seedling, and tea tree seedling is made to be rolled into strip.The bar shaped and tea tree seedling
It is consistent from root to the direction of terminal bud, and keep the high integrity of root, stem, leaf.Finally, obtaining Huang of looking for one's roots by bored yellow, dry
Tea head product.
The preparation of the oolong tea of looking for one's roots, first by the band root tea-tree tissue culture seedling proper alignment of collection in bamboo plaque, indoors
Heating deterioration or outdoor daylight or natural air drying wither, and after fried green, leaf rotation or booth blueness or fine manipulation of green tea leaves, are rubbed by manual or special
It twists equipment to rub band root seedling, so that tea tree seedling is rolled into strip or curly, finally bake, it is dry to obtain crow of looking for one's roots
Imperial tea head product.
The preparation of the dark green tea of looking for one's roots first finishes to tea tree seedling by baking oven or steam box, then passes through hand
Work to band root seedling carry out just rubs, after pile fermentation, rubbed again using special equipment of rubbing, make tea tree seedling be rolled into strip or
It is curly, finally bake, it is dry to obtain dark green tea head product of looking for one's roots.
The embodiment of the present invention is explained in detail above, but the present invention is not limited to above-mentioned embodiment party
Formula can also be made without departing from the purpose of the present invention within the knowledge of a person skilled in the art
Various change out.
Claims (10)
1. a kind of method for tea tissue cultures of looking for one's roots, it is characterised in that: include:
S1, the green young tender stem tip for choosing former tea tree plant, length 1-10cm；
S2, first by green young tender stem tip use on a sterile work bench tap water flowing water rinse 1-2h, be put under aseptic condition
30-60s, aseptic water washing 3-10 times are sterilized in 75-95% alcohol；It is subsequently placed in 0.1% mercuric chloride solution and sterilizes 3-15min,
Aseptic water washing 3-10 times；Finally, being divided into the stem-segment with node or stem apex of 0.5-1.5cm, it is inoculated in MS+6-BA0.1-5.0mg/
Initial culture is carried out in L+NAA0.01-0.1mg/L culture medium, and sterile in vitro tender shoots is obtained after 15-30 days；
S3, the stem apex or stem-segment with node that sterile in vitro tender shoots is cut to a length of 0.5-1.5cm transfer in culture medium MS+6-
Multiplying culture is carried out in BA0.5-5.0mg/L+NAA0.01-0.1mg/L；After 15-30 days, each stem apex or each stem-segment with node
All obtain the in vitro Multiple Buds of oneself；Multiple Buds are separated on a sterile work bench and are cut into single plant: part Multiple Buds switching in
In culture medium MS+6-BA0.5-5.0mg/L+NAA0.01-0.1mg/L, it to be used for shoot proliferation culture；Another part Multiple Buds turn
It is connected in culture medium MS+NAA0.01-1.0mg/L and carries out culture of rootage；After 15-30 days, the band root tea-tree tissue culture seedling of acquisition is used
In the raw material as tea preparation of looking for one's roots, the in vitro Multiple Buds of acquisition are then repeatedly used for above-mentioned shoot proliferation culture and training of taking root
It supports.
2. the method for tea tissue cultures according to claim 1 of looking for one's roots, it is characterised in that: the condition of tissue culture room is set
Are as follows: 15-28 DEG C of room temperature, intensity of illumination control is used in 1000-4000lx, the illumination of photoperiod 12-16h, dark phase 8-12h, light source
LED plant tissue culture white light Special energy-saving lamp.
3. the method for tea tissue cultures according to claim 2 of looking for one's roots, it is characterised in that: the temperature of condition of culture controls,
Specifically 22-28 DEG C of daytime can be regulated to according to the temperature variations in each place of production, 15-20 DEG C of evening.
4. the method for tea tissue cultures according to claim 1 of looking for one's roots, it is characterised in that: described for Initial culture, increasing
Grow culture, the MS minimal medium of shoot proliferation culture can also be B5 minimal medium；MS for culture of rootage is trained substantially
Feeding base can also be 1/2MS culture medium.
5. the method for tea tissue cultures according to claim 1 of looking for one's roots, it is characterised in that: described for Initial culture, increasing
Grow the plant hormone 6-BA and NAA of culture, shoot proliferation culture；Wherein, 6-BA can be basic element of cell division 6-BA, GA, BAP,
ZT, KT, 2,4-D are one of or several, and it is one of or several that NAA can be auxin NAA, IAA, IBA.
6. a kind of production method for tea of looking for one's roots, it is characterised in that: collect the band root tea-tree tissue culture of claim 1 the method culture
Seedling by rubbing or twisting device by hand, and combines the manufacture craft of traditional different cultivars tea, and production is looked for one's roots tea accordingly.
7. realizing the twisting device in the production method for tea of looking for one's roots described in claim 6, it is characterised in that: by rubbing plate lifting surely
Adjusting nut (1), manual lifting mechanism (2) rub plate (3) surely, move and rub layer (4), outfeed platforms (5), foot prop (6), rub surely
Twist layer (7), it is dynamic rub a layer subiculum platform (8), it is dynamic rub a layer pop-up platform (9), motor (10), belt (11), shaft (12),
Pan feeding platform (13) and dynamic layer pop-up platform support (14) of rubbing form；Entire twisting device is supported in work by foot prop (6)
It on table top, rubs a layer subiculum platform (8) and is located on foot prop (6), dynamic rub on layer is connected separately on the outside of each foot prop (6)
It holds in the palm platform support (14), is used to support to move and rubs a layer pop-up platform (9), dynamic rub is fixed with manual liter on layer pop-up platform (9)
Descending mechanism (2) is simultaneously connect with plate (3) is rubbed surely, is rubbed layer (7) surely and is adhered closely to rub plate (3) bottom surely, rubs plate (3) surely
And its lifting of layer (7) is rubbed in bottom surely, by manual lifting mechanism (2) top determine to rub plate lift adjustment nut (1) into
Row is adjusted, amplitude 2-10mm；It moves to rub layer subiculum platform (8) and move and rub between layer pop-up platform (9), motor is installed
(10) it and shaft (12) and is connected by belt (11), the rotation of shaft (12) drives the dynamic rotation for rubbing layer (4), rubs plate surely
(3) it is located to move and rubs layer top of pop-up platform (9) side, dynamic dynamic rub layer (4) for rubbing layer pop-up platform (9) other side is put down
Platform is as pan feeding platform (13).
8. twisting device according to claim 7, it is characterised in that: described dynamic rub layer (4) are annular rubber layer, perimeter
For 2-10m, a layer subiculum platform (8) is passively rubbed when mobile respectively, dynamic layer pop-up platform (9) of rubbing supports.
9. twisting device according to claim 7, it is characterised in that: described rub surely plate (3) are steel, the length is
0.5-4.5m, width 0.5-1.5m, height 0.02-0.1m, tea inlet side are designed at rounded corner；Surely layer (7) use is rubbed
Rubber or plank are made and rub surely the corrugated design of one side that layer (7) is contacted with tealeaves.
10. twisting device according to claim 7, it is characterised in that: the length of the pan feeding platform (13) is 0.5-4m,
Width is 0.5-1.5m.
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| CN114766366A (en) * | 2022-05-17 | 2022-07-22 | 河池学院 | Tissue culture method suitable for Liupao tea Shannu purple original seed seedlings |
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