CN110314116A - It is a kind of to cooperate with anti-oxidant oral care composition and its application - Google Patents
It is a kind of to cooperate with anti-oxidant oral care composition and its application Download PDFInfo
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- CN110314116A CN110314116A CN201810295503.4A CN201810295503A CN110314116A CN 110314116 A CN110314116 A CN 110314116A CN 201810295503 A CN201810295503 A CN 201810295503A CN 110314116 A CN110314116 A CN 110314116A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The invention discloses a kind of anti-oxidant oral care composition of collaboration, the composition includes the acceptable carrier of tea polyphenols magnolia obovata extract and oral cavity, wherein the weight fraction ratio of tea polyphenols magnolia obovata extract is 100:1-1:100.The present composition is acted synergistically by component tea polyphenols magnolia obovata extract, oxidation resistance is greatly improved, the present composition is than being used alone oxidation resistance increase rate Yue≤23%.
Description
Technical field
The present invention relates to oral care techniques field, more particularly, to a kind of anti-oxidant oral care composition of collaboration and its
Using.
Background technique
Human periodontal diseases are inflammatory conditions, are complicated interactions between periodontopathogens and host immune response
Result.It has been recognized that first is swashing for host immune system for progression of periodontal disease there are two related aspects
Living, second is the generation of oxygen radical and its associated metabolites.The increase that oxygen radical generates can facilitate oxidative stress, it is believed that
Periodontal disease is related with this.
Oulitis is to support the inflammation or infection of the gums and alveolar bone of tooth.It is generally acknowledged that oulitis is (special by oral bacteria
Be not the bacterium that dental plaque forms middle initiation) and as the bacterium by-product generate toxin caused by.Namely think dental plaque
The inflammation of oral cavity tissue in oral cavity is caused with bacteriotoxin.Compared with oulitis, periodontitis is the disease shape that progressive deteriorates
State wherein gingival inflammation and starts to shrink back from tooth, and forms pit in shrinking back, and finally can lead to bone and periodontal ligament
It destroys.Therefore, chronic infection and inflammation may cause subsequent absence of tooth.In addition, oral tissue inflammation can also be by following originals
Because causing: surgical operation, local damage, wound or necrosis or various systemic origins.
Cellular component involved in periodontal disease and oulitis etc. includes that epithelial tissue, Gingival Fibroblasts and circulation grain are thin
Born of the same parents, the host response for the virulence factor that all these cellular components facilitate directed toward bacteria to generate.Therefore, the bacterium sense of oral cavity tissue
Dye causes host immune response, and by generating radical type substance (levels of reactive oxygen species) and will lead to significant tissue damage
Inflammatory mediator is adjusted up, and reduces agglutination.
Free radical is the atom containing unpaired electron, lewis' acid, and is therefore usually unstable, and show
Shorter half-life period.Active oxygen (ROS) is the product generated in various biochemical processes, including superoxide anion (O2-), peroxide
Change hydrogen (H2O2), hydroxyl radical free radical (OH-) and non-free radical oxidant such as singlet oxygen (O2).The generation of ROS can be many thin
A part of born of the same parents' process, the cell processes include mitochondrial respiratory, immune cell responses, cellular damage, come from drug and its
The heat radiation etc. in many sources of his chemical substance metabolism.ROS is highly reactive, and modifies important cellular macromolecule,
ROS can cause or accelerate lysis.
For example, ROS is generated in inflammatory process by phagocytosis granulocyte, the phagocytosis granulocyte for example generates superoxide radical
The neutrophil leucocyte of the activation of " oxidative burst (oxidative burst) " is (it is believed that the superoxide radical is to generate institute
State the necessary factor of activated neutral granulocyte cellulotoxic effect).Moreover, superoxides can physiologically be given birth to by epithelial cell
At, for being reacted with physiological regulation agent nitrous oxide, generation peroxynitrite ONOO-, ONOO-It can decay and generate hydroxyl OH.
Other oxygen radical sources are: electronics " leakage ", the prostaglandin of mitochondria or endoplasmic reticular electron transfer chain from fracture close
At, the oxidation of catecholamine and platelet activation etc..
In short, ROS is related to nearly all lysis and ageing process.ROS generation under pathological conditions will increase, and pass through
By the effect of these high response molecules, by cross-linked proteins, mutagenized dna and makes lipid peroxidation, lead to cellular damage.
The another aspect of immune response is to generate various intracellular and intercellular inflammatory mediators.Its work to inflammatory reaction
It is the arachidonic acid metabolite generated via cyclooxygenase or lipoxygenase pathway with a kind of medium being widely studied, i.e.,
Prostaglandin and leukotrienes.These metabolites as primary medium be related in oulitis, periodontitis, osteomyelitis and its
His inflammatory disease.
Therefore, if it is desired to mammalian subject of the treatment with oral cavity tissue infection, inflammation and potential cellular damage, it can
The side such as pathogen infection is killed, the generation of one or more inflammatory mediators is reduced and reduces free radical activity oxygen substance to pass through
Method, to reduce the cellular damage to oral cavity tissue, to promote to treat.
Tea polyphenols (Tea Polyphenols) are the general name of Polyphenols of Tea, including flavanol compound, anthocyanin
Class, flavonoids, flavonols and phenolic acid class etc..Predominantly flavanols (catechin) class, catechin account for 60-80%.Catechin
Compound mainly includes catechin (EC), nutgall catechin (EGC), catechin and gallate (ECG) and galla turcica catechu
Plain 4 kinds of substances of gallate (EGCG).Substance tea polyphenols are also known as tea tan or tea tannin, are to form tealeaves color, smell and taste main
There is one of main ingredient of healthcare function in one of composition and tealeaves.Studies have shown that tea polyphenols isoreactivity substance tool removing toxic substances and
Radiation resistance can effectively stop radioactive substance intrusion marrow, and strontium 90 and Co 60 can be made to excrete rapidly, healthy
And medical field is described as " radiation jinx ".
Tea polyphenols have stronger antioxidation, and especially ester catechin EGCg, it is different bad that reproducibility is even up to L-
100 times of hematic acid.In 4 kinds of major catechin compounds, oxidation resistance EGCg > EGC > ECg > EC > BHA, and it is anti-oxidant
Performance enhances with the raising of temperature.Tea polyphenols in addition to antioxidation, also have bacteriostasis, such as to staphylococcus,
Escherichia coli, hay bacillus etc. have inhibiting effect.Peculiar smell in the adsorbable food of tea polyphenols, therefore make with certain deodorization
With.There is protective effect to the pigment in food, it can not only play the role of natural pigment, but also can prevent food from fading, and tea is more
Phenol also has the formation and summation for inhibiting nitrite.
Currently, tea polyphenolic compounds are mainly used for killing the lactic acid present in slot in oral care composition
Bacterium and other caries bacterias, have the function of inhibit glucose polymerisation enzymatic activity, such germ cannot on tooth implantation, effectively
The interruption process that forms saprodontia.The protein food remained in slot becomes the matrix of putrefactivebacteria proliferation, and tea is more
Phenol can kill such bacterium.Therefore it is used primarily as tooth antibacterial agent.
Magnolia cortex P.E typically refers to the dry of Cortex Magnoliae Officinalis (Magnolia officinalis belongs to one kind of Magnoliacea plant)
The equivalent of the extract or the extract or its active constituent of dry cortex or bark.It generally can be by using including second
Organic solvent including alcohol is extracted using supercritical extraction method, or is obtained by synthesis or semisynthesis.Cortex Magnoliae Officinalis
Extract has special, lasting myorelaxant effects and strong antibacterial action, can inhibit platelet aggregation.It is clinically main
As antibacterial, antifungal.In field of oral care, Magnolia cortex P.E also primarily as tooth antibacterial agent come using.
Summary of the invention
The application first technical problem to be solved is to provide a kind of anti-oxidant oral care composition of collaboration.This combination
Object passes through the synergistic effect of component tea polyphenols magnolia obovata extract, and oxidation resistance, the present composition is greatly improved
Than oxidation resistance increase rate Yue≤23% is used alone.
The application second technical problem to be solved is to provide a kind of application for cooperateing with anti-oxidant oral care composition.
In order to solve the first technical problem mentioned above, the present invention adopts the following technical scheme that:
A kind of anti-oxidant oral care composition of collaboration, includes at least tea polyphenols magnolia obovata extract, wherein tea polyphenols and
The weight fraction ratio of Magnolia cortex P.E is 100:1-1:100.
As the further improvement of technical solution, in the Magnolia cortex P.E comprising honokiol, tetrahydro honokiol or
Its derivative honokiol, tetrahydromagnolol or derivatives thereof.
Preferably, honokiol belongs to hydroxybiphenyl compounds, has the following structure:
Preferably, magnolol belongs to hydroxybiphenyl compounds, has the following structure:
Honokiol and magnolol isomers each other.
Preferably, the weight fraction ratio of the honokiol and magnolol is 1:9-9:1.
As the further improvement of technical solution, the tea polyphenols include one or more following compounds: galla turcica
Catechin gallate (EGCg), L-Epicatechin gallate (ECG), catechuic acid (Catechin), epicatechin
(Epicatechin, EC) and epigallocatechin (EGC) and chlorogenic acid；Wherein, ratio of the EGCg in tea polyphenols total weight
Li Wei≤30%.
Preferably, ratio Wei≤50% of the EGCg in tea polyphenols total weight；It is highly preferred that EGCg is in tea polyphenols total amount
Ratio Wei≤70%.
As the further improvement of technical solution, addition weight ratio of the tea polyphenols in oral care composition is
0.0001-3%.
It further include stannous ion source in the oral care composition as the further improvement of technical solution.
Preferably, the stannous ion source is selected from: stannous fluoride, stannous chloride, stannous pyrophosphate, phosphoric acid stannous chloride, bromination
Stannous, stannous iodide, stannous oxide, stannous sulfide, metaphosphoric acid stannous, stannous acetate, stannous tartrate, stannous citrate, apple
One of tartaric acid stannous, malonic acid stannous, gluconic acid stannous and stannous oxalate are a variety of.
Preferably, the weight ratio of the tea polyphenols and stannous ion source is 1:1-1:100.
It can further comprise abrasive, moisturizing in the oral care composition as the further improvement of technical solution
The auxiliary materials such as agent and/or thickener.
It can further comprise antibacterial agent, anti-caries in the oral care composition as the further improvement of technical solution
Agent, anti-sensitizer, antilithic and/or whitening agent isoreactivity ingredient.
To solve above-mentioned second technical problem, oral care composition of the present invention be can be applied in toothpaste or gargle.
Any range documented by the present invention includes any numerical value between end value and end value and end value or end value
Between any subrange for being constituted of any number.
Unless otherwise specified, each raw material in the present invention can be obtained by commercially available purchase, equipment used in the present invention
The conventional equipment in fields can be used or carried out referring to the prior art of fields.
Compared with prior art, the invention has the following beneficial effects:
The present composition is acted synergistically by component tea polyphenols magnolia obovata extract, and anti-oxidant energy is greatly improved
Power, the present composition is than being used alone oxidation resistance increase rate Yue≤23%.
Specific embodiment
In order to illustrate more clearly of the present invention, below with reference to preferred embodiment, the present invention is described further.Ability
Field technique personnel should be appreciated that following specifically described content is illustrative and be not restrictive, this should not be limited with this
The protection scope of invention.
A kind of anti-oxidant oral care composition of collaboration, includes at least tea polyphenols magnolia obovata extract, wherein tea polyphenols and
The weight fraction ratio of Magnolia cortex P.E is 100:1-1:100.
According to the certain embodiments of the application, the weight fraction ratio of the tea polyphenols magnolia obovata extract is 90:1-1:
90 or 80:1-1:80 or 70:1-1:70 or 60:1-1:60 or 50:1-1:50 or 40:1-1:40 or 30:1-1:30 or
20:1-1:20 or 10:1-1:10 or 5:1-1:5.
Applicant is found surprisingly that after mixing tea polyphenols magnolia obovata extract, within the scope of aforementioned proportion, the two has
Oxidation resistant ability is cooperateed with, the effect than exclusive use significantly improves.
According to the certain embodiments of the application, honokiol or derivatives thereof magnolia obovata is included in the Magnolia cortex P.E
Phenol or derivatives thereof.
According to the certain embodiments of the application, the honokiol belongs to hydroxybiphenyl compounds, has the following structure:
According to the certain embodiments of the application, the magnolol belongs to hydroxybiphenyl compounds, has the following structure:
Honokiol and magnolol isomers each other.
According to the certain embodiments of the application, the weight fraction ratio of the honokiol and magnolol be 1:9-9:1 or
1:8-8:1 or 1:7-7:1 or 1:6-6:1 or 1:5-5:1 or 1:4-4:1 or 1:3-3:1 or 1:2-2:1 or 2:9-9:
2 or 2:8-8:2 or 2:7-7:2 or 2:6-6:2 or 2:5-5:2 or 2:3-3:2 or 3:9-9:3 or 3:8-8:3 or 3:
7-7:3 or 3:6-6:3 or 3:5-5:3 or 3:4-4:3.
Applicants have found that the mixed collaboration oxidation resistance of tea polyphenols magnolia obovata extract, with Magnolia cortex P.E
In include that honokiol or derivatives thereof honokiol or derivatives thereof is related.And it also requires controlling the ratio of the amount of the two
Range.Within the scope of aforementioned proportion, tea polyphenols magnolia obovata extract, which has, cooperates with oxidation resistant ability, than the effect of exclusive use
It significantly improves.
According to the certain embodiments of the application, the tea polyphenols include one or more following compounds: galla turcica
Catechin gallate (EGCg), L-Epicatechin gallate (ECG), catechuic acid (Catechin), epicatechin
(Epicatechin, EC) and epigallocatechin (EGC) and chlorogenic acid；Wherein, ratio of the EGCg in tea polyphenols total weight
Li Wei≤30%.
According to certain preferred embodiments of the application, ratio Wei≤50% of the EGCg in tea polyphenols total weight；It is more excellent
Selection of land, ratio Wei≤70% of the EGCg in tea polyphenols total amount.
It has been investigated that when the content of EGCg in tea polyphenols is lower than 30%, between tea polyphenols magnolia obovata extract almost
Do not cooperate with oxidation resistance.As the Han Liang≤30% of EGCg in tea polyphenols, there is collaboration between tea polyphenols magnolia obovata extract
Oxidation resistance；As the Han Liang≤50% of EGCg in tea polyphenols, there is obvious collaboration antioxygen between tea polyphenols magnolia obovata extract
Change ability；As the Han Liang≤70% of EGCg in tea polyphenols, have between tea polyphenols magnolia obovata extract cooperate with significantly it is anti-oxidant
Ability.
According to the certain embodiments of the application, addition weight ratio of the tea polyphenols in oral care composition is
0.0001-3% or 0.001-3% or 0.01-3% or 0.1-3% or 0.3-3% or 0.5-3% or 0.7-3% or
0.9-3% or 1.1-3% or 1.3-3% or 1.5-3% or 1.7-3% or 1.9-3% or 2.1-3% or 2.3-
3% or 2.5-3% or 2.7-3% or 0.1-2% or 0.3-2% or 0.5-2% or 0.7-2% or 0.9-2% or
1.1-2% or 1.3-2% or 1.5-2% or 1.7-2% or 0.1-1% or 0.3-1% or 0.5-1% or 0.7-
1% or 0.9-1%.
It further include stannous ion source in the oral care composition according to the certain embodiments of the application.
According to certain preferred embodiments of the application, the stannous ion source is selected from: stannous fluoride, stannous chloride, coke
Phosphoric acid stannous chloride, phosphoric acid stannous chloride, stannous bromide, stannous iodide, stannous oxide, stannous sulfide, metaphosphoric acid stannous, stannous acetate, wine
One of stone acid stannous, stannous citrate, stannous malate, malonic acid stannous, gluconic acid stannous and stannous oxalate are more
Kind.
According to certain preferred embodiments of the application, the weight ratio of the tea polyphenols and stannous ion source is 1:1-1:
100。
For the stannous ion source as reducing agent, itself has certain oxidation resistance；Stannous ion source is added to
In the combination of tea polyphenols magnolia obovata extract, embody the collaboration oxidation resistance between all components more significantly and not only
It is only the antioxidative simple superposition of single raw material.
According to certain preferred embodiments of the application, can further comprise in the oral care composition abrasive,
The auxiliary materials such as moisturizer, flavoring agent and/or thickener.
According to certain preferred embodiments of the application, can further comprise in the oral care composition antibacterial agent,
Anti-caries agent, anti-sensitizer, antilithic and/or whitening agent isoreactivity ingredient.
Oral care composition of the present invention can be applied in toothpaste or gargle.
" abrasive " is the body material of toothpaste, typically constitutes from the 20%-50% of formula, most important in toothpaste to act on just
It is removal tartar, mitigates calculus dentalis.The selection of abrasive should consider its hardness, size, shape and content comprehensively, it is ensured that effectively clear
Clean general formula does not wear tooth.Exemplary abrasive may include but be not limited to below one or more: calcium carbonate, phosphoric acid hydrogen
Calcium, calcium pyrophosphate, silica, tricalcium phosphate, alumina silicate, aluminium hydroxide, aluminium oxide, zeolite, titanium oxide, silicic acid, kaolin
Deng.
" moisturizer " is that oral care composition is prevented to be dehydrated the ingredient being hardened.Exemplary moisturizer includes but is not limited to such as
Glycerol, propylene glycol, sorbierite, polyethylene glycol of low molecular weight etc..By mass percentage, moisturizer is in oral care composition
In content be usually 10-80%.
" thickener " is a kind of viscosity for increasing solution or liquid/solid mixtures, but will not substantially change its property
Substance.The purpose that thickener is added is to provide skeleton, mobility and stability to product.Exemplary thickener includes,
But it is not limited to, one or more of: hydroxyethyl cellulose, carboxymethyl cellulose and its salt (such as sodium carboxymethylcellulose),
Carrageenan (carrageenan), carboxyl vinyl polymer, xanthan gum (xanthan gum), carragheen, gelatin, branch
Starch, sodium alginate etc..In some embodiments, the thickener includes xanthan gum, carragheen or sodium carboxymethylcellulose
One of or it is a variety of.Weight ratio of the thickener in oral care composition is usually 0.2-2%.
" surfactant " is the purpose that emulsifying essence and blistering are played in toothpaste, can assist hydroxyl to a certain extent
Base apatite-multi-carboxy compound compound is abundant and is completely dispersed.Exemplary surfactant includes, but are not limited to yin
Ionic surface active agent, such as dodecyl sodium sulfate；Amphoteric surfactant, such as glycine betaine；Amino acid surfactant
Such as laruyl alcohol sodium sarcosinate and nonionic surfactant, such as polyoxyethylene and polyoxypropylene copolymers, polyethylene glycol etc..Table
Weight ratio of the bright activating agent in oral care composition is usually 0.5-2.5%.
It can further comprise antibacterial agent, anti-caries in the oral care composition according to the certain embodiments of the application
Agent, anti-sensitizer, antilithic and/or whitening agent isoreactivity ingredient；
" antibacterial agent " is the growth or breeding for referring to make within a certain period of time certain micro-organisms in oral care composition
It is maintained at necessary chemical substance below horizontal.Exemplary antibacterial agent includes but is not limited to: zinc oxide, stannous chloride, tetrahydro ginger
Flavine, cetylpyridinium chloride, triclosan etc..
" anti-caries agent " refers to the substance for having inhibiting effect to dental caries, such as can be by reducing enamel hydroxyapatite
Solubility enhances the substance of the anti-caries ability of tooth, or control bacterial plaque, the substance for inhibiting bacterial growth.Exemplary anti-caries agent includes
But it is not limited to, fluoride ion source (e.g., sodium fluoride, potassium fluoride, calcirm-fluoride, magnesium fluoride, stannous fluoride, ammonium fluoride, single fluorophosphoric acid
Sodium, single fluorophosphoric acid potassium, fluosilicate etc.), phosphorus reagent (calcium phosphate, sodium trimetaphosphate, magnesium glycerophosphate, calcium lactophosphate, junket egg
White acid sodium etc.) or arginine and its derivative.Preferably, in some specific embodiments, the anti-caries agent includes fluoride
Ion source.
" anti-sensitizer " refers to through inhibition nerve impulse or can close dentinal tubule or reduce its permeability, thus in advance
Anti- or treatment dental hypersensitiveness substance.Exemplary anti-sensitizer includes but is not limited to: dipotassium glycyrrhizinate, potassium fluoride, potassium nitrate,
The potassium ion sources such as potassium chloride.Preferably, in some specific embodiments, the anti-sensitizer includes potassium ion source.
" antilithic " refers to the metal ion complex that can play the role of anti-tooth calculus.Exemplary antilithic packet
It includes but is not limited to: pyrophosphate, tripolyphosphate, hexametaphosphate or citrate.
" whitening agent " refers to the substance that whitening function can be played to tooth.Exemplary whitening agent includes but is not limited to: peroxide
Compound bleaching agent, papain, glucose oxidase.
The detection method of the oxidation resistance of substance is as follows in the present invention:
Oxidation resistance can be assessed by radicals scavenging experimental method, most commonly seen radicals scavenging experiment side
Method is DPPH method；DPPH (1,1- diphenyl -2- trinitrophenyl-hydrazine) is a kind of free radical at very stable nitrogen center, is frequently used for
The in vitro anti-oxidation of antioxidant content is evaluated；DPPH method was suggested in 1958, be widely used in quantitative determination Biosample and
The oxidation resistance of food；This method is that have single electron according to DPPH free radical, there is the last one absorption at 517nm, and alcoholic solution is in
The characteristic of purple；In the presence of having free radical scavenger, so that its absorption is faded away due to matching with its single electron, fade
The electron amount that degree receives with it is at quantitative relationship, thus available spectrophotometer carries out quick quantitative analysis.
1. experimental material
1.1. reagent: ethyl alcohol, DPPH,
1.2. instrument: Shimadzu UV-1780
2. solution is prepared
Solution 1-1: Magnolia cortex P.E 100mg is weighed in 100ml volumetric flask, scale is dissolved and be settled to ethyl alcohol, is obtained
The Magnolia cortex P.E solution of 1mg/ml；Wherein the weight fraction ratio of honokiol and magnolol is 7:3.
Solution 1-2: Magnolia cortex P.E 100mg is weighed in 100ml volumetric flask, scale is dissolved and be settled to ethyl alcohol, is obtained
The Magnolia cortex P.E solution of 1mg/ml；Wherein the weight fraction ratio of honokiol and magnolol is 1:10.
Solution 1-3: Magnolia cortex P.E 100mg is weighed in 100ml volumetric flask, scale is dissolved and be settled to ethyl alcohol, is obtained
The Magnolia cortex P.E solution of 1mg/ml；Wherein the weight fraction ratio of honokiol and magnolol is 10:1.
Solution 2-1: weighing tea polyphenol extract object 100mg in 100ml volumetric flask, scale dissolved and be settled to ethyl alcohol,
Obtain the tea polyphenols solution of 1mg/ml；Wherein, ratio of the EGCg in tea polyphenols total weight is 25%.
Solution 2-2: weighing tea polyphenol extract object 100mg in 100ml volumetric flask, scale dissolved and be settled to ethyl alcohol,
Obtain the tea polyphenols solution of 1mg/ml；Wherein, ratio of the EGCg in tea polyphenols total weight is 30%.
Solution 2-3: weighing tea polyphenol extract object 100mg in 100ml volumetric flask, scale dissolved and be settled to ethyl alcohol,
Obtain the tea polyphenols solution of 1mg/ml；Wherein, ratio of the EGCg in tea polyphenols total weight is 50%.
Solution 2.4: weighing tea polyphenol extract object 100mg in 100ml volumetric flask, scale dissolved and be settled to ethyl alcohol,
Obtain the tea polyphenols solution of 1mg/ml；Wherein, ratio of the EGCg in tea polyphenols total weight is 70%.
Solution 3: it takes 1ml solution 1-1 into 100ml volumetric flask with pipette, scale is dissolved and be settled to ethyl alcohol, is obtained
The Magnolia cortex P.E solution of 0.01mg/ml；
Solution 4: it takes 1ml solution 2-3 into 100ml volumetric flask with pipette, scale is dissolved and be settled to ethyl alcohol, is obtained
The tea polyphenols solution of 0.01mg/ml；
Solution 5-1: taking 1ml solution 1-1 and 1ml solution 4 into 100ml volumetric flask with pipette, simultaneously with ethyl alcohol dissolution
Be settled to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 100:1；
Solution 5-2: taking 1ml solution 1-2 and 1ml solution 4 into 100ml volumetric flask with pipette, simultaneously with ethyl alcohol dissolution
Be settled to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 100:1；
Solution 5-3: taking 1ml solution 1-3 and 1ml solution 4 into 100ml volumetric flask with pipette, simultaneously with ethyl alcohol dissolution
Be settled to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 100:1；
Solution 6-1: taking 1ml solution 2-1 and 1ml solution 3 into 100ml volumetric flask with pipette, simultaneously with ethyl alcohol dissolution
Be settled to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 1:100；
Solution 6-2: taking 1ml solution 2-2 and 1ml solution 3 into 100ml volumetric flask with pipette, simultaneously with ethyl alcohol dissolution
Be settled to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 1:100；
Solution 6-3: taking 1ml solution 2-3 and 1ml solution 3 into 100ml volumetric flask with pipette, simultaneously with ethyl alcohol dissolution
Be settled to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 1:100；
Solution 6-4: taking 1ml solution 2-4 and 1ml solution 3 into 100ml volumetric flask with pipette, simultaneously with ethyl alcohol dissolution
Be settled to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 1:100；
Solution 7: it takes 1ml solution 1-1 and 10ml solution 4 into 100ml volumetric flask with pipette, is dissolved and determined with ethyl alcohol
Hold to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 10:1；
Solution 8: taking 1ml solution 1-1 and 0.5ml solution 4 into 100ml volumetric flask with pipette, simultaneously with ethyl alcohol dissolution
Be settled to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 100:0.5；
Solution 9: taking 1ml solution 2-3 and 1.1ml solution 3 into 100ml volumetric flask with pipette, simultaneously with ethyl alcohol dissolution
Be settled to scale, acquired solution Magnolia cortex P.E: tea polyphenols weight ratio is 1:110；
2.1.DPPH solution: 7.9mg DPPH is weighed in 20ml volumetric flask, is dissolved with ethyl alcohol and is settled to scale.Again will
It is diluted to 100 μm of ol/l with ethyl alcohol.
2.2. sample solution: taking 1ml solution 3,4,5-1,5-2,5-3,6-1,6-2,6-3,6-4,7 respectively, and 8,9 and respectively
10ml is dissolved and be settled to ethyl alcohol, obtains sample solution.
3. experimental method
1.3.1. skip test: taking 2ml sample solution, adds 2ml ethyl alcohol, after mixing, tests extinction at 516.5nm
Degree.
3.2. reference is tested: being taken 2ml DPPH solution, is added 2ml ethyl alcohol, after mixing, tests extinction at 516.5nm
Degree.
3.3. sample test: taking 2ml DPPH solution, add 2ml sample solution, after mixing, tests at 516.5nm
Absorbance.
Test result is as follows:
| Solution | MBE:T | H:M | EGCg% | DPPH clearance rate % |
| 3 | 100:0 | 7:3 | / | 32.6 |
| 4 | 0:100 | / | 30 | 67.21 |
| 5-1 | 100:1 | 7:3 | 50 | 50.19 |
| 5-2 | 100:1 | 1:10 | 50 | 45.52 |
| 5-3 | 100:1 | 10:1 | 50 | 53.97 |
| 6-1 | 1:100 | 7:3 | 25 | 70.11 |
| 6-2 | 1:100 | 7:3 | 30 | 82.78 |
| 6-3 | 1:100 | 7:3 | 50 | 88.72 |
| 6-4 | 1:100 | 7:3 | 70 | 95.66 |
| 7 | 100:10 | 7:3 | 50 | 72.15 |
| 8 | 100:0.5 | 7:3 | 50 | 33.15 |
| 9 | 1:110 | 7:3 | 50 | 87.26 |
In above-mentioned table: MBE:T=Magnolia cortex P.E: tea polyphenols
H:M=honokiol: magnolol.
By testing above-mentioned solution inoxidizability, it will thus be seen that thick within the scope of desired ratio in the present invention
There is apparent collaboration antioxidation between plain extract and tea polyphenols.The present composition is than being used alone Magnolia cortex P.E
Or oxidation resistance increase rate Yue≤23% of tea polyphenols.
Embodiment 1
A kind of dentifrice composition, the raw material including following weight percent:
Wherein, the EGCg ratio in tea polyphenols is 60%；The parts by weight of honokiol and magnolol in magnolol extract
Number is than being 5:5.
Inoxidizability detection:
1. experimental material
1.1. reagent: ethyl alcohol, DPPH (1,1- diphenyl -2- trinitrophenyl-hydrazine), above-mentioned toothpaste
1.2. instrument: Shimadzu UV-1780
2. solution is prepared
2.1.DPPH solution: 7.9mg DPPH is weighed in 20ml volumetric flask, is dissolved with ethyl alcohol and is settled to scale.Again will
It is diluted to 100 μm of ol/l with ethyl alcohol.
2.2. it prepares toothpaste solution: weighing toothpaste shown in 2g table 7, be separately added into 10ml water and 10ml ethyl alcohol, stir
After so that toothpaste is uniformly dispersed, it is centrifuged 15 minutes under 3rpm, takes supernatant.It takes supernatant 1ml in 100ml volumetric flask, uses second
Alcoholic solution is dissolved to scale, obtains toothpaste solution.
3. experimental method
3.2. luminosity measurement
3.2.1. reference is tested: being taken 2ml DPPH solution, is added 2ml ethyl alcohol, after mixing, tests and inhale at 516.0nm
Luminosity obtains A ginseng.
3.2.2. toothpaste sample is tested: 2ml DPPH solution is taken, 2ml toothpaste solution is added, after mixing,
Absorbance is tested under 516.0nm obtains A sample.
3.2.3. blank control is tested: taking 2ml toothpaste solution, 2ml ethyl alcohol is added and surveys at 516.0nm after mixing
Examination absorbance obtains A sky.
DPPH clearance rate: 82.76%.
Embodiment 2- embodiment 3
A kind of dentifrice composition, the raw material including following weight percent:
| Composition % | Embodiment 2 | Embodiment 3 |
| Sorbierite | 70 | 70 |
| CMC-Na | 0.5 | 0.5 |
| Tetrasodium pyrophosphate | 0.5 | 0.5 |
| Saccharin sodium | 0.15 | 0.15 |
| Sodium fluoride | 0.167 | 0.167 |
| Stannous fluoride | 0.1 | 0.1 |
| Silica | 16 | 16 |
| Tea extract | 0.01 | 0.01 |
| Essence | 1.05 | 1.05 |
| SLS | 2.2 | 2.2 |
| Magnolia cortex P.E | / | 0.3 |
| Water | Surplus | Surplus |
Such as embodiment 2 and embodiment 3, embodiment 3 is to be added to Magnolia cortex P.E on the basis of embodiment 2.
Above-mentioned formula is placed 3 months under the conditions of 40 DEG C, the EGCg content in detection formula.
It can be seen from above-described embodiment that tea polyphenols can be improved in tooth in Magnolia cortex P.E in addition to collaboration is anti-oxidant
Stability in paste formulation.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention.For those of ordinary skill in the art, may be used also on the basis of the above description
To make other variations or changes in different ways.Here all embodiments can not be exhaustive.It is all to belong to this hair
The obvious changes or variations that bright technical solution is extended out are still in the scope of protection of the present invention.
Claims (13)
1. a kind of anti-oxidant oral care composition of collaboration, it is characterised in that: the composition includes tea polyphenols magnolia obovata extract
And the acceptable carrier in oral cavity, wherein the weight fraction ratio of tea polyphenols magnolia obovata extract is 100:1-1:100.
2. cooperateing with anti-oxidant oral care composition according to claim 1, it is characterised in that: the tea polyphenols magnolia obovata mentions
Take object weight fraction ratio be 90:1-1:90 or 80:1-1:80 or 70:1-1:70 or 60:1-1:60 or 50:1-1:50,
Or 40:1-1:40 or 30:1-1:30 or 20:1-1:20 or 10:1-1:10 or 5:1-1:5.
3. according to claim 1 or the 2 anti-oxidant oral care compositions of any collaboration, it is characterised in that: the Cortex Magnoliae Officinalis mentions
It takes in object comprising honokiol or derivatives thereof and magnolol or derivatives thereof.
4. cooperateing with anti-oxidant oral care composition according to claim 3, it is characterised in that: the honokiol belongs to hydroxyl
Base biphenol compound, has the following structure:
5. cooperateing with anti-oxidant oral care composition according to claim 3, it is characterised in that: the magnolol belongs to hydroxyl
Biphenol compound has the following structure:
6. cooperateing with anti-oxidant oral care composition according to claim 3, it is characterised in that: the honokiol and Cortex Magnoliae Officinalis
The weight fraction ratio of phenol be 1:9-9:1 or 1:8-8:1 or 1:7-7:1 or 1:6-6:1 or 1:5-5:1 or 1:4-4:1 or
1:3-3:1 or 1:2-2:1 or 2:9-9:2 or 2:8-8:2 or 2:7-7:2 or 2:6-6:2 or 2:5-5:2 or 2:3-3:
2 or 3:9-9:3 or 3:8-8:3 or 3:7-7:3 or 3:6-6:3 or 3:5-5:3 or 3:4-4:3.
7. cooperateing with anti-oxidant oral care composition according to claim 1, it is characterised in that: the tea polyphenols include one kind
Or a variety of following compounds: EGCg, ECG, catechuic acid, epicatechin, EGC, chlorogenic acid；Wherein, EGCg is in tea polyphenols total weight
In ratio Wei≤30%.
8. cooperateing with anti-oxidant oral care composition according to claim 7, it is characterised in that: the EGCg is total in tea polyphenols
Ratio Wei≤50% in weight.
9. cooperateing with anti-oxidant oral care composition according to claim 7, it is characterised in that: the EGCg is total in tea polyphenols
Ratio Wei≤70% in amount.
10. cooperateing with anti-oxidant oral care composition according to claim 7, it is characterised in that: the tea polyphenols are in oral cavity
Addition weight ratio in care composition is 0.0001-3% or 0.001-3% or 0.01-3% or 0.1-3% or 0.3-
3% or 0.5-3% or 0.7-3% or 0.9-3% or 1.1-3% or 1.3-3% or 1.5-3% or 1.7-3% or
1.9-3% or 2.1-3% or 2.3-3% or 2.5-3% or 2.7-3% or 0.1-2% or 0.3-2% or 0.5-
2% or 0.7-2% or 0.9-2% or 1.1-2% or 1.3-2% or 1.5-2% or 1.7-2% or 0.1-1% or
0.3-1% or 0.5-1% or 0.7-1% or 0.9-1%.
11. cooperateing with anti-oxidant oral care composition according to claim 1, it is characterised in that: the oral care combination
It further include stannous ion source in object.
12. cooperateing with anti-oxidant oral care composition according to claim 11, it is characterised in that: the stannous ion source choosing
From: stannous fluoride, stannous chloride, stannous pyrophosphate, phosphoric acid stannous chloride, stannous bromide, stannous iodide, stannous oxide, stannous sulfide,
Metaphosphoric acid stannous, stannous acetate, stannous tartrate, stannous citrate, stannous malate, malonic acid stannous, gluconic acid stannous,
One of stannous oxalate is a variety of.
13. cooperateing with anti-oxidant oral care composition according to claim 11, it is characterised in that: the tea polyphenols and stannous
The weight ratio of ion source is 1:1-1:100.
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| CN102046158A (en) * | 2008-05-30 | 2011-05-04 | Wm.雷格利Jr.公司 | Oral compositions containing enhanced antibacterial combinations of antioxidants and extracts of magnolia |
| CN106074192A (en) * | 2016-07-01 | 2016-11-09 | 好来化工(中山)有限公司 | Antioxidative oral care composition |
| CN106889171A (en) * | 2017-01-05 | 2017-06-27 | 四川膳缘食品有限公司 | A kind of food preservative and its fresh-keeping foodstuff |
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|---|---|---|---|---|
| CN102046158A (en) * | 2008-05-30 | 2011-05-04 | Wm.雷格利Jr.公司 | Oral compositions containing enhanced antibacterial combinations of antioxidants and extracts of magnolia |
| CN106074192A (en) * | 2016-07-01 | 2016-11-09 | 好来化工(中山)有限公司 | Antioxidative oral care composition |
| CN106889171A (en) * | 2017-01-05 | 2017-06-27 | 四川膳缘食品有限公司 | A kind of food preservative and its fresh-keeping foodstuff |
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