CN110300803B - 提高细胞基因组中同源定向修复(hdr)效率的方法 - Google Patents

提高细胞基因组中同源定向修复(hdr)效率的方法 Download PDF

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CN110300803B
CN110300803B CN201780086730.XA CN201780086730A CN110300803B CN 110300803 B CN110300803 B CN 110300803B CN 201780086730 A CN201780086730 A CN 201780086730A CN 110300803 B CN110300803 B CN 110300803B
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hdr
cell
nuclease
sequence
cells
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CN110300803A (zh
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J·N·费德
郭齐
G·A·敏泰
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Bristol Myers Squibb Co
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Crystallography & Structural Chemistry (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
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  • Developmental Biology & Embryology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201780086730.XA 2016-12-20 2017-12-20 提高细胞基因组中同源定向修复(hdr)效率的方法 Active CN110300803B (zh)

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CN202410548057.9A CN118460529A (zh) 2016-12-20 2017-12-20 提高细胞基因组中同源定向修复(hdr)效率的方法

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US201662437042P 2016-12-20 2016-12-20
US62/437,042 2016-12-20
PCT/US2017/067569 WO2018119060A1 (en) 2016-12-20 2017-12-20 Methods for increasing the efficiency of homology directed repair (hdr) in the cellular genome

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CN110300803A CN110300803A (zh) 2019-10-01
CN110300803B true CN110300803B (zh) 2024-05-14

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US (1) US20230193321A1 (https=)
EP (2) EP4286523A3 (https=)
JP (3) JP2020510443A (https=)
KR (2) KR20230162134A (https=)
CN (2) CN110300803B (https=)
ES (1) ES2962711T3 (https=)
WO (1) WO2018119060A1 (https=)

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US11597924B2 (en) 2016-03-25 2023-03-07 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
ES2962711T3 (es) 2016-12-20 2024-03-20 Bristol Myers Squibb Co Métodos para aumentar la eficacia de la reparación dirigida por homología (HDR) en el genoma celular
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
JP2020537515A (ja) * 2017-10-03 2020-12-24 ジュノー セラピューティクス インコーポレイテッド Ｈｐｖ特異的結合分子
WO2020014577A1 (en) * 2018-07-13 2020-01-16 Allele Biotechnology And Pharmaceuticals, Inc. Methods of achieving high specificity of genome editing
CA3144106A1 (en) 2019-07-03 2021-01-07 Integrated Dna Technologies, Inc. Identification, characterization, and quantitation of crispr-introduced double-stranded dna break repairs
CN119193713A (zh) 2019-10-24 2024-12-27 合成Dna技术公司 修饰的双链供体模板
CN111549052A (zh) * 2020-05-07 2020-08-18 西南大学 一种真核生物CRISPR/Cas全基因组编辑载体文库及构建方法
US20230257768A1 (en) * 2020-06-12 2023-08-17 National Institute Of Advanced Industrial Science And Technology Poultry cell in which a gene encoding a protein of interest is knocked-in at egg white protein gene, and method for producing said poultry cell
CN111944847A (zh) * 2020-08-28 2020-11-17 西北农林科技大学 一套等位基因高效替换系统及其建立方法
CN113416730B (zh) * 2021-07-07 2023-04-04 中国医学科学院血液病医院(中国医学科学院血液学研究所) 一种降低细胞因基因编辑产生的大片段删除突变的方法
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Publication number Publication date
EP3559230A1 (en) 2019-10-30
KR20190095412A (ko) 2019-08-14
JP2020510443A (ja) 2020-04-09
WO2018119060A1 (en) 2018-06-28
CN110300803A (zh) 2019-10-01
KR20230162134A (ko) 2023-11-28
US20230193321A1 (en) 2023-06-22
JP2024009934A (ja) 2024-01-23
EP4286523A2 (en) 2023-12-06
ES2962711T3 (es) 2024-03-20
JP2026012785A (ja) 2026-01-27
EP4286523A3 (en) 2024-03-20
CN118460529A (zh) 2024-08-09
EP3559230B1 (en) 2023-09-06

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