CN110293118A - A method of utilizing plant and the biological prosthetic oil contaminated soil of brilliant glue - Google Patents
A method of utilizing plant and the biological prosthetic oil contaminated soil of brilliant glue Download PDFInfo
- Publication number
- CN110293118A CN110293118A CN201910571545.0A CN201910571545A CN110293118A CN 110293118 A CN110293118 A CN 110293118A CN 201910571545 A CN201910571545 A CN 201910571545A CN 110293118 A CN110293118 A CN 110293118A
- Authority
- CN
- China
- Prior art keywords
- plant
- contaminated soil
- microorganism
- oil
- degradation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a kind of methods using plant and the biological prosthetic oil contaminated soil of brilliant glue.The method is using the brilliant glue microorganism renovation agent of the herbaceous plant such as clover or polyphyll G. paniculata and fixed oil degradation bacterium and fungi as object; it is metabolized using the symplastic growth of plant and microorganism; carry out the degradation of contaminated soil Crude Oil; crude oil pollution object is removed, to realize the biological prosthetic of contaminated soil.Method provided by the invention is easy to operate; high to the degradation clearance rate of crude oil pollution object, repairing efficiency is short, and industrial applications are convenient; suitable for, to the biology in situ reparation of crude oil pollution object, having broad application prospects in oil exploitation and field of environment protection under the soil environment of inland.
Description
Technical field
The invention belongs to environmental protection technical fields, and in particular to a kind of to utilize plant and the biological prosthetic crude oil pollution of brilliant glue
The method of soil.
Background technique
The reparation of oil-polluted soils is a world-famous puzzle, has carried out the research of many decades to the relevant technologies both at home and abroad,
Such as physical absorption of the method for proposition or emulsion dispersion elution, chemical breakdown and microbial degradation etc..But physical method and chemical method without
Method thoroughly removes petroleum hydrocarbon, at high cost, and large-area treatment difficulty is big, and often has secondary pollution；Microbial method can thoroughly degrade stone
Oil, it is at low cost, it can implement on a large scale, but degradation speed is slow.
In recent years, microorganism and the plant cooperated important technology direction repaired as domestic and international oil-polluted soils reparation.
But breeding of the degrading microorganism in contaminated soil, is limited to soil moisture, nutritional substrate, temperature, pH value, inorganic salts and stone
The environmental conditions such as the content of petroleum hydrocarbon and composition, existence and growth metabolism are frequently subjected to inhibit and cannot play a role.
Substrate release is cooperateed with balance, microbe survival, growth metabolism, plant growth, plant with microorganism in soil
Degradation etc., is all the critical bottleneck technology for determining biological prosthetic effect, and urgent need will be researched and solved.Meanwhile the prior art pair
The degradation clearance rate of crude oil pollution object is low, and repairing efficiency is long, and effect is undesirable.In addition, the original position for oil-polluted soils is repaired
It is multiple, then it acquires a certain degree of difficulty.
Summary of the invention
For the above-mentioned problems in the prior art, plant and brilliant glue biology are utilized it is an object of that present invention to provide a kind of
The method for repairing oil contaminated soil.
Present invention provide the technical scheme that
A method of utilizing plant and the biological prosthetic oil contaminated soil of brilliant glue.It is characterized in that, the method is with grass
The brilliant glue microorganism renovation agent of this plant and fixed oil degradation microorganism is object, utilizes the symplastic growth of plant and microorganism
Metabolism carries out the degradation of contaminated soil Crude Oil, crude oil pollution object is removed, to realize the biology in situ reparation of contaminated soil.
The method using plant and the biological prosthetic oil contaminated soil of brilliant glue, which is characterized in that the draft
Plant is clover or polyphyll G. paniculata.
The method using plant and the biological prosthetic oil contaminated soil of brilliant glue, which is characterized in that the fixation
The brilliant glue of oil degradation microorganism is the granular starch composite crystal for embedding the Mixed Microbes such as Rhodococcus sp and the saccharomycete of degradable petroleum
Glue microorganism renovation agent.
The method using plant and the biological prosthetic oil contaminated soil of brilliant glue, which is characterized in that described is granular
Starch base crystalline substance glue microorganism renovation agent is the brilliant glue microorganism renovation agent of CSETIA-BR series, fully biodegradable.
Further, the method using plant and the biological prosthetic oil contaminated soil of brilliant glue, which is characterized in that described
The step of method, is as follows:
1) by the brilliant glue microorganism renovation agent of a certain amount of fixed oil degradation microorganism, it is put in proportion into crude oil pollution to be repaired
In soil, it is uniformly mixed；
2) nutriment of plant growth and microorganism growth is added, then the smooth mixed soil being made with a thickness of 20 ~ 30cm；
3) vegetable seeds is sowed in mixed soil, or plant transplanting is kept the skin wet in mixed soil；
4) degradation conditions such as suitable illumination, ventilation, moisturizing are kept, and necessary substrate and nutriment etc. can be supplemented as needed,
Maintain plant growth and microbial metabolism；After certain repair time, by the synergistic effect of plant growth and microbial degradation,
Realize the degradation and removing of crude oil pollution object.
It is described using plant and brilliant glue biological prosthetic oil contaminated soil method the step of, which is characterized in that the step
It is rapid 1) in the ratio of brilliant glue microorganism renovation agent dosage (by mass) and soil volume be 0.8 ~ 8 kg/m3；
It is described using plant and brilliant glue biological prosthetic oil contaminated soil method the step of, which is characterized in that the step 2
In nutriment be carbon source and nitrogen source substrate etc. needed for compound fertilizer containing N, P, K etc. needed for plant growth and microorganism growth often
The ratio of the arbitrary proportion mixture of rule substrate, nutriment dosage (by mass) and soil volume is 0.08 ~ 4 kg/m3。
It is described using plant and brilliant glue biological prosthetic oil contaminated soil method the step of, which is characterized in that the step
It is rapid 4) in the degradation conditions such as illumination, ventilation, moisturizing be natural conditions；The repair time is 1 ~ 3 month.
By using above-mentioned technology, the invention has the following beneficial effects:
1) biological renovation method provided by the invention changes contaminated soil microenvironment using plant growth and root system, utilizes brilliant glue
Immobilized microorganism is degraded, and the cometabolism and degradation of plant and microorganism can be given full play to, and realizes petroleum wastewater
Contaminate the biology in situ reparation of soil.
2) biological renovation method provided by the invention, can be improved microorganism and exist the advantages of making full use of brilliant glue renovation agent
Survival ability in contaminated soil improves the biomass of thallus in soil, and growth metabolism is vigorous, to promote crude oil pollution
The degradation rate of object.
3) biological renovation method provided by the invention, repairing efficiency is short, can be high to the degradation clearance rate of crude oil pollution object
Up to 75 ~ 99%.
4) restorative procedure provided by the invention does not form secondary pollution, safe and efficient, and industrialization amplification and application are easy,
Repair process cost can be reduced, suitable for the contaminated soil biology in situ repair process during the oil exploitation of inland, in petroleum
The fields such as industry and environmental protection have broad application prospects.
Specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This:
Embodiment 1
By the CSETIA-BR series starch composite crystal glue microorganism renovation agent of 1 kg fixed Rhodococcus sp and saccharomycete, investment 1.25
m3It is in situ to be uniformly mixed in oil contaminated soil, be 4.8%(w/w through gravimetric method detection contaminated soil oil content, similarly hereinafter)；It is added
0.1 kg of nutriment such as carbon source and nitrogen source substrate needed for compound fertilizer and microorganism growth containing N, P, K etc. needed for plant growth,
Then the smooth mixed soil being made with a thickness of 20 ~ 30cm；Polyphyll G. paniculata seed is sowed in mixed soil, keep the skin wet dimension
Hold seed sprouting；Keep the degradation conditions such as suitable illumination, ventilation, moisturizing, natural temperature about 20 ~ 35oC maintains plant growth
And microbial metabolism；After repairing 1 month, by the synergistic effect of plant growth and microbial degradation, detects and pollute through gravimetric method
Soil oil content is 1.2%, and the degradation of crude oil pollution object and clearance rate are up to 75%.
Embodiment 2
By the CSETIA-BR series starch composite crystal glue microorganism renovation agent of 6 kg fixed Rhodococcus sp and saccharomycete, 3 m are put into3
It is in situ to be uniformly mixed in oil contaminated soil, it is 3% through gravimetric method detection contaminated soil oil content；It is added needed for plant growth
0.3 kg of the nutriment such as carbon source and nitrogen source substrate, then smooth that thickness is made needed for compound fertilizer and microorganism growth containing N, P, K etc.
Degree is the mixed soil of 20 ~ 30cm；Alfalfa seed is sowed in mixed soil, keeps the skin wet and seed is maintained to sprout；It keeps
The degradation conditions such as suitable illumination, ventilation, moisturizing, natural temperature about 20 ~ 35oC maintains plant growth and microbial metabolism；It repairs
After 1 month multiple, by the synergistic effect of plant growth and microbial degradation, it is through gravimetric method detection contaminated soil oil content
0.6%, the degradation of crude oil pollution object and clearance rate are up to 80%.
Embodiment 3
By the CSETIA-BR series starch composite crystal glue microorganism renovation agent of 20 kg fixed Rhodococcus sp and saccharomycete, investment 2.5
m3It is in situ to be uniformly mixed in oil contaminated soil, it is 0.3% through gravimetric method detection contaminated soil oil content；Plant growth institute is added
10 kg of the nutriment such as carbon source and nitrogen source substrate, then smooth to be made needed for the compound fertilizer containing N, P, K etc. needed and microorganism growth
With a thickness of the mixed soil of 20 ~ 30cm；Polyphyll G. paniculata seed is sowed in mixed soil, keeps the skin wet and seed is maintained to sprout；
Keep the degradation conditions such as suitable illumination, ventilation, moisturizing, natural temperature about 20 ~ 35oC maintains plant growth and microorganism generation
It thanks；After repairing 3 months, by the synergistic effect of plant growth and microbial degradation, contaminated soil oil content is detected through gravimetric method
It is 0.001%, the degradation of crude oil pollution object and clearance rate are removed up to 99% close to all degradations.
Content described in this specification is only to enumerate to inventive concept way of realization, and protection scope of the present invention is not answered
When the concrete form for being seen as limited by embodiment and being stated.
Claims (8)
1. a kind of method using plant and the brilliant biological prosthetic oil contaminated soil of glue, which is characterized in that the method be with
The brilliant glue microorganism renovation agent of herbaceous plant and fixed oil degradation microorganism is object, raw using the collaboration of plant and microorganism
Long metabolism, carries out the degradation of contaminated soil Crude Oil, crude oil pollution object is removed, to realize that the biology in situ of contaminated soil is repaired
It is multiple.
2. the method according to claim 1 using plant and the biological prosthetic oil contaminated soil of brilliant glue, which is characterized in that
The herbaceous plant is clover or polyphyll G. paniculata.
3. the method according to claim 1 using plant and the biological prosthetic oil contaminated soil of brilliant glue, which is characterized in that
The brilliant glue of the fixation oil degradation microorganism is the granular of the Mixed Microbes such as Rhodococcus sp and the saccharomycete of fixed degradable petroleum
Starch composite crystal glue microorganism renovation agent.
4. the method according to claim 1 using plant and the biological prosthetic oil contaminated soil of brilliant glue, which is characterized in that
The step of the method, is as follows: 1) by the brilliant glue microorganism renovation agent of a certain amount of fixed oil degradation microorganism, being put in proportion into
In oil contaminated soil to be repaired, it is uniformly mixed；2) nutriment of plant growth and microorganism growth is added, it is then smooth to be made
With a thickness of the mixed soil of 20 ~ 30cm；3) vegetable seeds is sowed in mixed soil, or plant is transplanted in mixed soil
In, it keeps the skin wet；4) degradation conditions such as suitable illumination, ventilation, moisturizing are kept, and can supplement as needed necessary substrate and
Nutriment etc. maintains plant growth and microbial metabolism；After certain repair time, pass through the association of plant growth and microbial degradation
Same-action realizes the degradation and removing of crude oil pollution object.
5. the step of oil contaminated soil method biological prosthetic with brilliant glue using plant according to claim 4, feature
It is, brilliant glue microorganism renovation agent dosage is by mass 0.8 ~ 8 kg/m with the ratio of soil volume in the step 1)3。
6. the step of oil contaminated soil method biological prosthetic with brilliant glue using plant according to claim 4, feature
It is, the nutriment in the step 2 is that the compound fertilizer and microorganism needed for plant growth containing N, P, K etc. grow required carbon
The arbitrary proportion mixture in source and nitrogen source conventional substrate, nutriment dosage are by mass 0.08 ~ 4 kg/ with the ratio of soil volume
m3。
7. the step of oil contaminated soil method biological prosthetic with brilliant glue using plant according to claim 4, feature
It is, the degradation conditions such as illumination, ventilation, moisturizing in the step 4) are natural conditions；The repair time is 1 ~ 3
Month.
8. the method according to claim 3 using plant and the biological prosthetic oil contaminated soil of brilliant glue, which is characterized in that
The granular starch base crystalline substance glue microorganism renovation agent is the brilliant glue microorganism renovation agent of CSETIA-BR series, can completely biological drop
Solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910571545.0A CN110293118A (en) | 2019-06-28 | 2019-06-28 | A method of utilizing plant and the biological prosthetic oil contaminated soil of brilliant glue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910571545.0A CN110293118A (en) | 2019-06-28 | 2019-06-28 | A method of utilizing plant and the biological prosthetic oil contaminated soil of brilliant glue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110293118A true CN110293118A (en) | 2019-10-01 |
Family
ID=68029235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910571545.0A Pending CN110293118A (en) | 2019-06-28 | 2019-06-28 | A method of utilizing plant and the biological prosthetic oil contaminated soil of brilliant glue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110293118A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112547792A (en) * | 2020-11-23 | 2021-03-26 | 陕西欧菲德环保科技有限公司 | Water locking agent for petroleum-polluted soil remediation and application thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2290270C1 (en) * | 2005-07-27 | 2006-12-27 | Федеральное государственное унитарное предприятие Межотраслевой научно-исследовательский институт экологии топливно-энергетического комплекса (ФГУП МНИИЭКО ТЭК) | Method of biological remediation of oil-polluted lands |
| CN101890426A (en) * | 2010-07-19 | 2010-11-24 | 南开大学 | Method for restoring petroleum-polluted saline alkali soil by ryegrass and high efficiency microbes |
| CN101974446A (en) * | 2010-07-29 | 2011-02-16 | 滨州学院 | Salt-tolerant Rhodococcus sp. JH for generating biological emulsifier and degrading alkane and application thereof in bioremediation of petroleum polluted saline-alkali soil |
| CN103523928A (en) * | 2013-07-16 | 2014-01-22 | 中国人民解放军海军医学研究所 | Bacterial agent for bioremediation of oil-polluted water area and preparation method |
| CN105382020A (en) * | 2015-12-09 | 2016-03-09 | 辽宁石油化工大学 | Application of babysbreath and organic phosphonic acid to strengthening treatment of lead-polluted soil |
| CN105478460A (en) * | 2016-01-12 | 2016-04-13 | 山东佳星环保科技有限公司 | Bioremediation method of oil-contaminated soil |
| CN108435789A (en) * | 2018-06-20 | 2018-08-24 | 江苏天象生物科技有限公司 | A kind of restorative procedure of soil |
| CN109746260A (en) * | 2017-11-03 | 2019-05-14 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
-
2019
- 2019-06-28 CN CN201910571545.0A patent/CN110293118A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2290270C1 (en) * | 2005-07-27 | 2006-12-27 | Федеральное государственное унитарное предприятие Межотраслевой научно-исследовательский институт экологии топливно-энергетического комплекса (ФГУП МНИИЭКО ТЭК) | Method of biological remediation of oil-polluted lands |
| CN101890426A (en) * | 2010-07-19 | 2010-11-24 | 南开大学 | Method for restoring petroleum-polluted saline alkali soil by ryegrass and high efficiency microbes |
| CN101974446A (en) * | 2010-07-29 | 2011-02-16 | 滨州学院 | Salt-tolerant Rhodococcus sp. JH for generating biological emulsifier and degrading alkane and application thereof in bioremediation of petroleum polluted saline-alkali soil |
| CN103523928A (en) * | 2013-07-16 | 2014-01-22 | 中国人民解放军海军医学研究所 | Bacterial agent for bioremediation of oil-polluted water area and preparation method |
| CN105382020A (en) * | 2015-12-09 | 2016-03-09 | 辽宁石油化工大学 | Application of babysbreath and organic phosphonic acid to strengthening treatment of lead-polluted soil |
| CN105478460A (en) * | 2016-01-12 | 2016-04-13 | 山东佳星环保科技有限公司 | Bioremediation method of oil-contaminated soil |
| CN109746260A (en) * | 2017-11-03 | 2019-05-14 | 中国石油化工股份有限公司 | Soil remediation composition and application and geobiont repair method |
| CN108435789A (en) * | 2018-06-20 | 2018-08-24 | 江苏天象生物科技有限公司 | A kind of restorative procedure of soil |
Non-Patent Citations (1)
| Title |
|---|
| 赵梁军: "《观赏植物生物学（第2版）》", 31 August 2011, 中国农业大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112547792A (en) * | 2020-11-23 | 2021-03-26 | 陕西欧菲德环保科技有限公司 | Water locking agent for petroleum-polluted soil remediation and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhang et al. | Aragonite formation induced by open cultures of microbial consortia to heal cracks in concrete: Insights into healing mechanisms and crystal polymorphs | |
| Meng et al. | Full-scale of composting process of biogas residues from corn stover anaerobic digestion: Physical-chemical, biology parameters and maturity indexes during whole process | |
| Bohutskyi et al. | Effects of inoculum size, light intensity, and dose of anaerobic digestion centrate on growth and productivity of Chlorella and Scenedesmus microalgae and their poly-culture in primary and secondary wastewater | |
| CN109207413B (en) | A kind of highly effective petroleum degradation composite bacteria agent and the preparation method and application thereof | |
| Das et al. | Microalgal nutrients recycling from the primary effluent of municipal wastewater and use of the produced biomass as bio-fertilizer | |
| Qiu et al. | Remediation of cadmium-contaminated soil with biochar simultaneously improves biochar’s recalcitrance | |
| CN109526681B (en) | Special nutritional type soil improvement matrix for saline-alkali earth root flower planting | |
| CN106006978B (en) | The substrate improved materials and preparation method and restorative procedure of a kind of high organic bed mud shallow lake | |
| CN102319729A (en) | New method for combined remediation of compound contaminated soil | |
| Raketh et al. | Sulfate removal using rubber wood ash to enhance biogas production from sulfate-rich wastewater generated from a concentrated latex factory | |
| CN111808754A (en) | Mining area soil microalgae and separation and purification method and application thereof | |
| CN112893430A (en) | Method for soil remediation by using coal-based solid waste remediation material | |
| Hasanudin | Palm oil mill effluent recycling system for sustainable palm oil industries | |
| CN110293118A (en) | A method of utilizing plant and the biological prosthetic oil contaminated soil of brilliant glue | |
| CN102699017A (en) | Method for repairing oil polluted wetland soil environment with cetylic acid intensified scirpi | |
| Liu et al. | Recovery of phosphorus via harvesting phosphorus-accumulating granular sludge in sequencing batch airlift reactor | |
| CN110484266B (en) | Sludge-based soil conditioner and preparation method thereof | |
| CN116495893A (en) | Sulfur autotrophic denitrification active microorganism carrier and application | |
| CN106905982A (en) | Using the heavy metal pollution alkaline land modifying agent and saline and alkali land improvement method of spent bleaching clay | |
| EP1856000A1 (en) | Sludge from sewage treatment plants | |
| CN102836869A (en) | Method for restoring crude oil-polluted soil by using sludge generated in yeast production | |
| CN114480194B (en) | Solid waste resource utilization method combining microorganisms with full-bionic simulation | |
| CN115537366A (en) | Microbial agent for biochar coupling synergistic degradation of TCC (TCC) and preparation method thereof | |
| CN114134069A (en) | Pseudomonas fluorescens N1 and application thereof | |
| Udume et al. | Composting: A low-cost biotechnological approach to ameliorating macrophyte nuisance in fresh waters |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191001 |