CN110291189A - 单细胞人多潜能干细胞的传代和收获制剂 - Google Patents

单细胞人多潜能干细胞的传代和收获制剂 Download PDF

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Publication number
CN110291189A
CN110291189A CN201880007148.4A CN201880007148A CN110291189A CN 110291189 A CN110291189 A CN 110291189A CN 201880007148 A CN201880007148 A CN 201880007148A CN 110291189 A CN110291189 A CN 110291189A
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cell
preparation
cells
stem cell
culture
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CN201880007148.4A
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English (en)
Chinese (zh)
Inventor
J·A·罗利
Y·聂
T·费尔纳
P·沃尔什
B·艾哈曼迪巴格巴德拉尼
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Waxman Lunsar Ltd By Share Ltd
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Waxman Lunsar Ltd By Share Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201880007148.4A 2017-01-17 2018-01-17 单细胞人多潜能干细胞的传代和收获制剂 Pending CN110291189A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762447262P 2017-01-17 2017-01-17
US62/447,262 2017-01-17
PCT/US2018/014032 WO2018136500A1 (en) 2017-01-17 2018-01-17 Passaging and harvesting formulation for single-cell human pluripotent stem cells

Publications (1)

Publication Number Publication Date
CN110291189A true CN110291189A (zh) 2019-09-27

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CN201880007148.4A Pending CN110291189A (zh) 2017-01-17 2018-01-17 单细胞人多潜能干细胞的传代和收获制剂

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Country Link
US (1) US20190359946A1 (https=)
EP (1) EP3555264A1 (https=)
JP (1) JP7092309B2 (https=)
KR (1) KR102580997B1 (https=)
CN (1) CN110291189A (https=)
CA (1) CA3050259A1 (https=)
IL (1) IL267628B2 (https=)
WO (1) WO2018136500A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114807018A (zh) * 2022-02-28 2022-07-29 深圳市旷逸生物科技有限公司 一种多能干细胞的传代方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220113349A (ko) * 2019-12-11 2022-08-12 리페아론 게엠베하 생물반응기의 현탁액에서 줄기세포의 확장

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110097799A1 (en) * 2009-10-19 2011-04-28 Casey Stankewicz Cardiomyocyte production
CA2831609A1 (en) * 2011-03-30 2012-10-04 Cellular Dynamics International, Inc. Priming of pluripotent stem cells for neural differentiation
US20140212966A1 (en) * 2011-05-17 2014-07-31 Ying Nie Passaging and harvesting formulation and method for human pluripotent stem cells
US20150265652A1 (en) * 2014-03-21 2015-09-24 Cellular Dynamics International, Inc. Production of midbrain dopaminergic neurons and methods for the use thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110097799A1 (en) * 2009-10-19 2011-04-28 Casey Stankewicz Cardiomyocyte production
CA2831609A1 (en) * 2011-03-30 2012-10-04 Cellular Dynamics International, Inc. Priming of pluripotent stem cells for neural differentiation
US20140212966A1 (en) * 2011-05-17 2014-07-31 Ying Nie Passaging and harvesting formulation and method for human pluripotent stem cells
US20150265652A1 (en) * 2014-03-21 2015-09-24 Cellular Dynamics International, Inc. Production of midbrain dopaminergic neurons and methods for the use thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
韩建永等: "哺乳动物PGCs用于多能性干细胞培养的研究进展", 《黑龙江畜牧兽医》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114807018A (zh) * 2022-02-28 2022-07-29 深圳市旷逸生物科技有限公司 一种多能干细胞的传代方法
CN114807018B (zh) * 2022-02-28 2023-08-01 深圳市旷逸生物科技有限公司 一种多能干细胞的传代方法

Also Published As

Publication number Publication date
IL267628A (en) 2019-08-29
JP7092309B2 (ja) 2022-06-28
US20190359946A1 (en) 2019-11-28
EP3555264A1 (en) 2019-10-23
WO2018136500A1 (en) 2018-07-26
JP2020513765A (ja) 2020-05-21
KR102580997B1 (ko) 2023-09-21
IL267628B2 (en) 2025-06-01
IL267628B1 (en) 2025-02-01
KR20190104407A (ko) 2019-09-09
CA3050259A1 (en) 2018-07-26

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