CN110283243B - 大腹园蛛牵引丝性能及其丝蛋白基因序列 - Google Patents
大腹园蛛牵引丝性能及其丝蛋白基因序列 Download PDFInfo
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Abstract
本发明公开了大腹园蛛牵引丝性能及其丝蛋白基因序列，丝蛋白氨基酸序列为SEQ ID NO:1、SEQ ID NO:2或/和SEQ ID NO:3或/和SEQ ID NO:4所示，或者其氨基酸序列为将SEQ ID NO:1、SEQ ID NO:2或/和SEQ ID NO:3或/和SEQ ID NO:4所述的多肽经过一个或几个氨基酸残基的取代、缺失或添加而获得的且有等同力学性能的氨基酸序列，力学性能包括延伸性、强度和/或韧性。大腹园蛛牵引丝蛋白MaSp1和MaSp2的蛋白和基因序列，大腹园蛛牵引丝的力学性能非常出色，韧性强，为后续理解不同物种、不同性能蛛丝蛋白进化格局奠定基础，利于开发超级蛛丝蛋白。
Description
技术领域
本发明涉及大腹园蛛牵引丝及其丝蛋白基因序列。
背景技术
蜘蛛丝是自然界强度优异、阻尼性良好并且弹性突出的一类天然丝纤维，被誉为“生物钢”，是一类极具应用潜力的生物质材料。尤其在军工、医药等领域。但目前丝蛋白新材料研发领域仍相对落后，缺乏自主知识产权资源。蜘蛛丝是自然界中一类极具应用潜力的生物质材料，蜘蛛丝的种类、数目繁多，结构特征各异，其中，牵引丝蛋白(主要是MaSp)由于其强度、韧性综合性能最优，而成为广受关注的一类蛛丝蛋白。
由于蜘蛛特殊的生物学特性，人工饲养蜘蛛已获得大量蛛丝蛋白并不可行。蜘蛛丝的种类、数目繁多，结构特征各异，相应的，蜘蛛丝蛋白的种类也很丰富，远比于另一类泌丝昆虫鳞翅目昆虫的丝蛋白更为复杂。然而目前可获得的蛛丝蛋白基因资源非常有限，尤其是更具有深入分析意义的长序列资源。大腹园蛛，虽然目前已发表了2种牵引丝蛋白(主要由朱壶腹腺丝MaSp组成)的基因序列，但是相对较短，迫切需要全长基因信息，才能得以全面把握丝蛋白的结构特征；另一方面，由于缺乏基因组学数据信息，该类蛋白基因是否存在其他基因家族成员，是否存在独特的剪切形式，还未知。这些信息的获得，迫切需要结合长读长的测序手段，通过全长转录组的系统解析，以全面发掘牵引丝蛋白序列。
发明内容
本发明在充分比较牵引丝性能的基础上，利用第三代测序技术，进行大腹园蛛等3种物种的蜘蛛进行全长转录组测序，并结合比较转录组学理论技术及生物信息学分析，充分挖掘大腹园蛛的牵引丝蛋白基因序列。
本发明的目的在于提供大腹园蛛牵引丝蛋白序列和基因序列及其应用。
本发明所采取的技术方案是：
一种蛋白质，其氨基酸序列为SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3或/和SEQID NO:4所示；或者其氨基酸序列为将SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3或/和SEQID NO:4所述的多肽经过一个或几个氨基酸残基的取代、缺失或添加而获得的且具有等同力学性能的氨基酸序列，所述力学性能包适延伸性、强度和/或韧性。
一种蛋白质，其由SEQ ID NO:1～4所示的蛋白质组成。
一种蛋白质，其由蛋白质A、蛋白质B、蛋白质C和蛋白质D组成，其中编码蛋白质A的核苷酸序列如SEQ ID NO:5所示，编码蛋白质B的核苷酸序列如SEQ ID NO:6所示，编码蛋白质C的核苷酸序列如SEQ ID NO:7，编码蛋白质D的核苷酸序列如SEQ ID NO:8所示。
编码上述所述蛋白质的核酸。
编码上述所述蛋白质的核酸，其核苷酸序列为SEQ ID NO:5、SEQ ID NO:6、SEQ IDNO:7或/和SEQ ID NO:8所示。
含有上述所述核酸的重组载体。
用上述所述的重组载体转化或转染的宿主细胞。
上述所述蛋白质的制备方法，其包括用上述所述的宿主细胞表达上述所述的蛋白质，然后分离上述所述的蛋白质。
SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3或/和SEQ ID NO:4所示的蛋白质在人造材料中的应用。
一种人造材料，其含有SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3或/和SEQ ID NO:4所示的蛋白质。
本发明的有益效果是：
本发明首次发现了大腹园蛛牵引丝蛋白MaSp1的全长，其长度显著长于已发表序列的蛋白序列，以及MaSp2的三种不同的剪切体序列，并发现大腹园蛛牵引丝的力学性能非常出色，其强度很高、延伸性也较高，具有很好的韧性，显著优于其他种蜘蛛的牵引丝。本发明为后续深入理解不同物种、不同性能蛛丝蛋白进化格局的奠定了基础，为开发超级蛛丝蛋白奠定了重要的理论，提供了重要的分子素材。
附图说明
图1不同蜘蛛牵引丝的力学性能测试不同颜色的曲线代表不同的生物学重复数据；横坐标为延伸率(0-30％)，纵坐标为拉伸强度(0-1400mpa)，拉伸应力-应变曲线所围成的面积代表韧性；从图中可以清楚的看出，大腹园蛛牵引丝的曲线面积高于其他物种。
图2为MaSp1的氨基酸组成分析。
图3为MaSp2(即MaSp2a)的氨基酸组成分析。
图4为大腹园蛛MaSp1和MaSp2三种剪切体(即MaSp2a、MaSp2b和MaSp2c)氨基酸序列的分析，其中图A为MaSp1，图B为MaSp2c，图C为MaSp2a和MaSp2b。
具体实施方式
下面结合具体实施例对本发明作进一步的说明。
本发明首先在陕西、广东、云南三地系统采集了结网性能有差异的圆网蜘蛛物种，并进行丝性能评估，结果显示园蛛科大腹园蛛牵引丝MaSp性能优越。进一步选择了4种代表性物种进行了全长转录组测序，结合系统搜集已发表蛛丝蛋白序列，在大腹园蛛测序的物种中发掘到一批蛛丝蛋白基因资源。对三种蜘蛛的MaSp序列比较，发现大腹园蛛牵引丝MaSp存在较为独特的基序；MaSp1的polyA尾后往往会跟随1-3个丝氨酸；另外，MaSp2的三种发现了三种剪切体形式(MaSp2a，MaSp2b，MaSp2c)。这三种蛋白在以polyA为结尾的cassete重复结构上，存在很大的差异。MaSp2a和MaSp2b的cassete结构比MaSp1和MaSp2c更加复杂。
本发明首次发现了大腹园蛛牵引丝MaSp1的全长蛋白序列和基因序列，以及MaSp2的的三种剪切体，其中两种以前均未发表过，另一种早前只发表了很短的序列。并发现大腹园蛛牵引丝的力学性能非常出色，其韧性非常强，显著优于其他几种广泛被认为有较优丝性能的蜘蛛的牵引丝韧性。本发明为后续深入理解不同物种、不同性能蛛丝蛋白进化格局的奠定了基础，为开发超级蛛丝蛋白奠定了重要的理论及技术平台。
实施例1大腹园蛛牵引丝MaSp的蛋白序列和基因序列
MaSp(Major ampullate silk，主壶腹腺丝)又称牵引丝，由MaSp1和MaSp2组成，MaSp1不含有脯氨酸，主要功能用于逃逸，织网，编织网框，构成网骨架的框丝，具有很高的强度。目前，大腹园蛛的MaSp1和MaSp2的全长序列还未被发表过，目前圆蛛科中有发表金珠属的横纹金珠的MaSp1(945个aa)，MaSp2(3360)，其他亲缘物种的物种如肖峭科的络新妇蜘蛛MaSp2(2466个aa)，黑寡妇蜘蛛的MaSp1(3123个aa)，MaSp2(3779个aa)，其他序列由于长度过低，无法评估其基因特征。
我们通过测序发掘到了大腹园蛛牵引丝中的MaSp1氨基酸序列如SEQ ID NO:1所示，一共1651个氨基酸，具有全长，从结构上来看，其有N端，有C端，是更完整的序列。MaSp2有三种亚型，分别记为MaSp2a、MaSp2b和MaSp2c，MaSp2a的氨基酸序列如SEQ ID NO:2所示，一共1588个氨基酸；MaSp2b的氨基酸序列如SEQ ID NO:3所示，一共1469个氨基酸，MaSp2c的氨基酸序列如SEQ ID NO:4所示，一共1250个氨基酸。本发明获得的编码MaSp1的核苷酸序列如SEQ ID NO:5所示，编码MaSp2a、MaSp2b、MaSp2c的核苷酸序列分别如SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8所示。我们通过与其他物种的比对，与亲缘物种相比，我们保证了序列的可靠性，同时又有一定的差异，而差异性显现了其蜘蛛的结网差别，揭示了蜘蛛物种进化关系(如图2所示)。
通过三种蜘蛛(大腹园蛛，络新妇，云斑蛛)的三代全长转录组数据的分析，我们目前完成了MaSp的进一步序列确认。我们对三种MaSp蛋白重复区的氨基酸序列基序进行了种间分析，发现的确存在间的共性和差异(图2、图3和图4)。
首先，MaSp重复区基序(motif)，共性是：C端均存在多个丙氨酸(A)序列，masp1与masp2最大的差异是，masp2有多个GPGGX的结构，而在masp1中却几乎不存在脯氨酸P,在masp1中，大腹园蛛在每个基序的polyA后有着丝氨酸，然而在masp2中，络新妇MaSp在基序的N端多出GA序列，大腹园蛛则在基序的种间有两段明显的氨基酸序列插入(图2、图3和图4)，这可能与种间的重复区基序与丝纤维的性能有关。
图4为大腹园蛛MaSp1和MaSp2三种剪切体(即MaSp2a、MaSp2b和MaSp2c)氨基酸序列的分析，其中图4-A为MaSp1，图4-B为MaSp2c，图4-C为MaSp2a和MaSp2b。MaSp1的polyA尾后往往会跟随1-3个丝氨酸，另外，MaSp2的三种剪切体蛋白在以polyA为结尾的cassete重复结构存在很大的差异。MaSp2c与MaSp1的cassete结构比较类似，只有一长一短两个简单的cassete结构。MaSp2a和MaSp2b，其cassete结构更加复杂。主要表现在MaSp2a和MaSp2b包含多种cassete结构，而这些cassete结构与MaSp1和MaSp2c的cassete结构不相同。MaSp2a和MaSp2b又多个cassete结构是共用的，如cassete B、cassete D、cassete E、cassete F、cassete G；而MaSp2a和MaSp2b又分别具有自己特有的cassete结构，如MaSp2a特有的cassete A、cassete C，以及MaSp2b特有的cassete H、cassete I。其中的cassete J为MaSp2a和MaSp2b中不具有重复性的cassete结构。这些不同的cassete结构在氨基酸数目以及motif组成上都存在一定的差异。
上述分析结果进一步说明，与近缘物种相比，本专利发现的大腹园蛛牵引丝MaSp1和MaSp2a和MaSp2b的序列具有准确性，同时又有一定的差异，而差异性显现了其蜘蛛的结网性能差别，为进一步设计超级蛛丝蛋白奠定了基础。
实施例2大腹园蛛牵引丝的力学性能分析
方法：采用instron拉力机对各种蜘蛛(大腹园蛛、金珠、棘腹蛛、络新妇)的牵引丝进行了力学性能测试,测试条件:温度：24-26℃，湿度：75-90％,抽丝速度为10.57cm/s。同时用扫描电镜(S4800 1.0kV 6.6mm×9.00k SE(U))测量蜘蛛丝直径，根据应力＝P/A,应变＝L-L0/L0(P为载荷,A为蜘蛛丝的初始截面积),对于每一蜘蛛丝试样测试多次，数据的统计处理主要使用origin2015软件完成,最终得到在不同拉伸强度下的蜘蛛丝的力学数据。
我们对圆网蜘蛛(大腹园蛛、金珠、棘腹蛛、络新妇)进行了力学性能测试，保持其他因素不变的情况下，最终得到拉伸应力-应变曲线如下图1所示。
从力学性能检测结果数据和如图1曲线结果可以看出，大腹园蛛延伸性很高(24.498±4.593％)，明显高于金蛛(16.677±3.677％)和棘腹蛛(20.009±3.010％)，但略低于络新妇(27.023±3.440％)。同样大腹园蛛牵引丝的强度也很高(1073.714±201.391MPa)，与金蛛(1220.564±268.868MPa)较为接近，但明显高于其棘腹蛛(242.609±41.250MPa)和络新妇(1069.019±98.197MPa)。总而言之，从图中的拉伸应力-应变曲线格局也可以看出，大腹园蛛的曲线面积高于其他物种，与之相关联的韧性最强。综上所述，大腹园蛛牵引丝的综合力学性能优异。
上述实施例为本发明较佳的实施方式，但本发明的实施方式并不受上述实施例的限制，其他的任何未背离本发明的精神实质与原理下所作的改变、修饰、替代、组合、简化，均应为等效的置换方式，都包含在本发明的保护范围之内。
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Gly Arg Gly Ala Gly Ala Gly Gly Tyr Gly Gln Gly Gly Ser Gly
1010 1015 1020
Thr Ala Ala Ala Ala Ala Ala Ser Ser Ser Gly Ser Gly Ser Asp
1025 1030 1035
Gly Tyr Gly Gly Gly Tyr Asn Gly Gln Gly Gly Thr Gly Gln Gly
1040 1045 1050
Gly Ser Gly Ala Ala Ala Ala Ala Ala Gly Gly Gly Gly Ser Gly
1055 1060 1065
Gly Ser Gly Gly Arg Gly Ala Gly Ala Gly Gly Tyr Gly Gln Gly
1070 1075 1080
Gly Ser Gly Ser Ala Ala Ala Ala Ala Ser Ser Ser Gly Ser Gly
1085 1090 1095
Gly Asp Gly Tyr Gly Gly Gly Tyr Asn Gly Gln Gly Gly Pro Gly
1100 1105 1110
Gln Gly Gly Ser Gly Ala Ala Ala Ala Ala Ala Ser Gly Gly Gly
1115 1120 1125
Ser Gly Gly Ser Gly Gly Arg Gly Ala Gly Ala Gly Arg Tyr Gly
1130 1135 1140
Gln Gly Gly Ser Gly Ser Ala Ala Ala Ala Ala Ala Ser Ser Ile
1145 1150 1155
Gly Ser Gly Ser Asp Gly Tyr Gly Gly Gly Tyr Asn Gly Gln Gly
1160 1165 1170
Gly Pro Gly Gln Gly Gly Ser Gly Ala Ala Ala Ala Ala Ala Ser
1175 1180 1185
Gly Gly Gly Ser Gly Gly Ser Gly Gly Arg Gly Ala Gly Ala Gly
1190 1195 1200
Arg Tyr Gly Arg Gly Gly Ser Gly Ser Ala Ala Ala Ala Ala Ala
1205 1210 1215
Ser Ser Ser Gly Ser Gly Ser Asp Gly Tyr Gly Gly Gly Tyr Asn
1220 1225 1230
Gly Gln Gly Gly Pro Gly Gln Gly Gly Ser Ser Ala Ala Ala Ala
1235 1240 1245
Ala Gly Ser Gly Gly Gly Ser Gly Gly Phe Gly Gly Arg Gly Ala
1250 1255 1260
Gly Ala Gly Gly Tyr Gly Gln Gly Gly Ser Gly Ser Ala Ala Ser
1265 1270 1275
Ala Ala Ser Ser Ser Gly Ser Gly Gly Asp Gly Tyr Gly Gly Gly
1280 1285 1290
Tyr Asn Gly Gln Gly Gly Pro Gly Gln Gly Gly Ser Gly Ala Ala
1295 1300 1305
Ala Ala Ala Ala Ser Gly Gly Arg Ser Gly Gly Ser Gly Gly Arg
1310 1315 1320
Gly Ala Gly Ala Gly Gly Tyr Gly Gln Gly Gly Ser Gly Ser Ala
1325 1330 1335
Ala Ala Ala Ala Ala Ser Ser Ser Gly Ser Gly Ser Asp Gly Tyr
1340 1345 1350
Gly Gly Gly Tyr Asn Gly Gln Gly Gly Pro Gly Gln Gly Gly Ser
1355 1360 1365
Gly Ala Ala Ala Ala Ala Ala Ala Ser Gly Gly Glu Ser Gly Gly
1370 1375 1380
Ser Gly Gly Arg Gly Ala Gly Ser Gly Gly Tyr Gly Leu Gly Gly
1385 1390 1395
Ser Gly Ser Ala Ala Ala Ala Ala Ala Ser Ser Ser Gly Ser Gly
1400 1405 1410
Ser Asp Arg Tyr Gly Gly Gly Tyr Lys Gly Gln Gly Gly Ser Gly
1415 1420 1425
Ser Ala Ala Ala Ala Ala Ala Ser Ser Asn Gly Ser Gly Ser Asp
1430 1435 1440
Gly Tyr Gly Gly Gly Tyr Asn Gly Gln Gly Gly Pro Gly Gln Gly
1445 1450 1455
Gly Ser Val Ala Ala Ala Ala Ala Ala Ser Arg Gly Gly Ser Gly
1460 1465 1470
Gly Phe Gly Gly Arg Gly Val Ser Pro Gly Gly Tyr Gly Lys Gly
1475 1480 1485
Ser Thr Gly Ser Ser Ser Ala Ala Ala Ala Ser Phe Asp Ala Ser
1490 1495 1500
Gly Ser Asp Gly Tyr Gly Arg Glu Phe Gly Gly Gln Gly Gly Ser
1505 1510 1515
Gly Gln Asn Gly Ala Ser Ala Ala Thr Ser Ser Ser Ser Gly Gly
1520 1525 1530
Gly Phe Gly Gly Tyr Gly Ala Gly Gln Gly Val Tyr Gly Gly Gly
1535 1540 1545
Leu Gln Glu Pro Ala Ala Ala Ser Ala Ala Val Ala Ser Ser Ala
1550 1555 1560
Ala Ser Arg Leu Ser Ser Pro Ser Ser Arg Ser Arg Val Ser Ser
1565 1570 1575
Ala Val Ser Ile Phe Leu Asp Asn Gly Leu Arg Ser Pro Glu Ala
1580 1585 1590
Phe Ser Ser Val Tyr Asn Ser Phe Val Ser Asp Ile Ile Ser Ser
1595 1600 1605
Asn Pro Asn Leu Ser Gly Cys Glu Val Leu Val Gln Val Leu Leu
1610 1615 1620
Glu Ile Leu Ala Ala Thr Ile Ser Gly Leu Ser Gly Leu Asp Ser
1625 1630 1635
Asn Ser Ser Met Asn Val Val Lys Ser Phe Val Ser
1640 1645 1650
<210> 2
<211> 1587
<212> PRT
<213> 大腹园蛛
<400> 2
Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala
1 5 10 15
Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln
20 25 30
Gly Pro Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly
35 40 45
Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser
50 55 60
Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln
65 70 75 80
Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala Gly
85 90 95
Gly Tyr Gly Pro Gly Gly Gly Gln Gln Gly Pro Gly Gly Gln Gly Thr
100 105 110
Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro
115 120 125
Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly
130 135 140
Ser Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly
145 150 155 160
Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Val Ala Ala
165 170 175
Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gln Gln Gly
180 185 190
Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala
195 200 205
Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly
210 215 220
Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly
225 230 235 240
Ala Ser Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser
245 250 255
Gly Gln Gln Gly Pro Gly Gly Gln Gly Ser Gly Gly Ala Gly Gln Gln
260 265 270
Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala Ala
275 280 285
Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro
290 295 300
Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro
305 310 315 320
Gly Ala Ser Ala Ala Val Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly
325 330 335
Ser Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro
340 345 350
Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr
355 360 365
Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gly Gln Gly Ser Tyr Gly
370 375 380
Thr Gly Ala Ser Ala Ser Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro
385 390 395 400
Gly Gly Gly Gln Gln Gly Pro Gly Gly Gln Gly Thr Gly Gly Ala Gly
405 410 415
Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser Ala Ala
420 425 430
Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Pro Gly Gln Gln
435 440 445
Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr
450 455 460
Gly Pro Ala Ala Ser Ala Ala Ala Ala Val Ala Ala Gly Gly Tyr Gly
465 470 475 480
Pro Gly Ser Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gly Gln
485 490 495
Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly
500 505 510
Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln
515 520 525
Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala
530 535 540
Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly
545 550 555 560
Pro Gly Gly Gln Gly Ser Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly
565 570 575
Gln Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala
580 585 590
Gly Gly Tyr Gly Leu Gly Ser Gly Gln Gln Gly Pro Gly Gly Ala Gly
595 600 605
Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala
610 615 620
Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln
625 630 635 640
Gly Pro Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly
645 650 655
Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser
660 665 670
Gly Gln Gln Gly Pro Gly Gly Gln Gly Ser Tyr Gly Thr Gly Ala Ser
675 680 685
Ala Ser Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Gly Gly Gln
690 695 700
Gln Gly Pro Gly Gly Gln Gly Ser Gly Gly Ala Gly Gln Gln Gly Pro
705 710 715 720
Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala
725 730 735
Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gly
740 745 750
Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Gly
755 760 765
Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Gly Gln Gly
770 775 780
Pro Gly Ser Gly Gly Gln Gln Gly Pro Ser Gly Gln Gly Pro Tyr Gly
785 790 795 800
Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro
805 810 815
Gly Ser Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Thr Gly
820 825 830
Gly Gln Gly Pro Tyr Gly Pro Gly Val Ser Ala Ala Ala Ser Ala Ala
835 840 845
Ala Gly Gly Tyr Gly Pro Gly Gly Gly Gln Gln Gly Ser Gly Gly Gln
850 855 860
Gly Ser Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr
865 870 875 880
Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly
885 890 895
Pro Gly Ser Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro
900 905 910
Gly Gly Gln Gly Pro Tyr Gly Pro Gly Gly Ala Ala Ala Ala Ala Ala
915 920 925
Ala Gly Gly Tyr Gly Pro Gly Gly Gln Gly Pro Gly Ser Gly Gly Gln
930 935 940
Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala
945 950 955 960
Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly
965 970 975
Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly
980 985 990
Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro
995 1000 1005
Gly Ser Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gly Gln
1010 1015 1020
Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Val
1025 1030 1035
Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gly Ala
1040 1045 1050
Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala
1055 1060 1065
Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ala Gly
1070 1075 1080
Gly Pro Gln Gly Pro Gly Gly Ala Gly Pro Gln Gly Pro Ser Gly
1085 1090 1095
Pro Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly
1100 1105 1110
Ala Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Arg
1115 1120 1125
Gly Gly Ala Gly Pro Gly Ser Val Gly Gln Gln Gly Ser Ser Gly
1130 1135 1140
Ala Gly Gln Tyr Gly Pro Gly Ala Ala Ala Ala Ala Ala Ala Ala
1145 1150 1155
Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gln Gln
1160 1165 1170
Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala
1175 1180 1185
Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln
1190 1195 1200
Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro
1205 1210 1215
Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly
1220 1225 1230
Gly Tyr Gly Pro Gly Gly Gly Gln Gln Gly Pro Gly Gly Gln Gly
1235 1240 1245
Thr Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Val Pro Tyr
1250 1255 1260
Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr
1265 1270 1275
Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln
1280 1285 1290
Gly Pro Gly Gly Gln Gly Ser Tyr Gly Pro Gly Ala Ser Ala Ala
1295 1300 1305
Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Gln Gln Gly Pro
1310 1315 1320
Arg Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro
1325 1330 1335
Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala
1340 1345 1350
Ala Ala Ala Gly Gly Tyr Gly Pro Gly Gln Gln Gly Pro Arg Gln
1355 1360 1365
Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gly
1370 1375 1380
Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala Gly
1385 1390 1395
Gly Tyr Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly
1400 1405 1410
Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ser
1415 1420 1425
Ala Ala Ala Ala Ala Ala Ser Gly Gly Tyr Gly Pro Gly Gln Gln
1430 1435 1440
Gly Pro Gly Gln Gln Gly Arg Gly Gln Gln Gly Pro Gly Gly Gln
1445 1450 1455
Gly Pro Tyr Gly Pro Gly Ala Ala Ser Ala Ala Val Ser Val Gly
1460 1465 1470
Gly Tyr Gly Pro Gln Arg Ser Ser Ala Pro Val Ala Ser Ala Ala
1475 1480 1485
Ala Ser Arg Leu Ser Ser Pro Ala Ala Ser Ser Arg Val Ser Ser
1490 1495 1500
Ala Val Ser Ser Leu Val Ser Ser Gly Pro Ser Asn Pro Ala Ala
1505 1510 1515
Leu Ser Ser Thr Ile Ser Ser Ala Val Ser Gln Ile Ser Ala Ser
1520 1525 1530
Asn Pro Gly Leu Ser Gly Cys Asp Val Leu Val Gln Ala Leu Leu
1535 1540 1545
Glu Val Val Ser Ala Leu Val His Ile Leu Gly Ser Ser Ser Ile
1550 1555 1560
Gly Gln Ile Asn Tyr Gly Ala Ser Ser Gln Tyr Ala Gln Met Val
1565 1570 1575
Gly Gln Ser Val Ala Gln Ala Leu Gly
1580 1585
<210> 3
<211> 1468
<212> PRT
<213> 大腹园蛛
<400> 3
Gln Gln Gly Pro Gly Gly Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly
1 5 10 15
Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly
20 25 30
Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly
35 40 45
Gly Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Gly Gly Ala Gly Gln
50 55 60
Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser Ala Ser Ala
65 70 75 80
Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly
85 90 95
Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly
100 105 110
Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro
115 120 125
Gly Gly Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Gly Gly Ala Gly
130 135 140
Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly
145 150 155 160
Pro Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly
165 170 175
Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln
180 185 190
Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln
195 200 205
Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala
210 215 220
Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Gly Gly Gln Gln Gly Pro
225 230 235 240
Gly Gly Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Ala
245 250 255
Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser Ala
260 265 270
Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln
275 280 285
Gln Gly Pro Gly Gly Glu Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala
290 295 300
Ala Ala Ala Ala Ala Gly Gly Tyr Arg Pro Gly Gly Gly Gln Gln Gly
305 310 315 320
Pro Gly Gly Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly
325 330 335
Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser
340 345 350
Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly
355 360 365
Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly
370 375 380
Pro Gly Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln
385 390 395 400
Gly Thr Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly
405 410 415
Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser
420 425 430
Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln
435 440 445
Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala Gly
450 455 460
Gly Tyr Gly Pro Gly Gly Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro
465 470 475 480
Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro
485 490 495
Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly
500 505 510
Ser Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Ser
515 520 525
Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala
530 535 540
Gly Gly Tyr Gly Pro Gly Gly Gly Gln Gln Gly Pro Gly Gly Gln Gly
545 550 555 560
Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly
565 570 575
Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala
580 585 590
Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly
595 600 605
Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ala Ala
610 615 620
Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Thr Gly Gly Gln
625 630 635 640
Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr
645 650 655
Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Gly Ala Gly Gly Tyr Gly
660 665 670
Pro Gly Ser Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro
675 680 685
Gly Ser Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala
690 695 700
Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gln
705 710 715 720
Gln Gly Pro Gly Gly Gln Gly Leu Tyr Gly Pro Gly Gly Ala Ala Ala
725 730 735
Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro
740 745 750
Gly Gly Gln Gly Pro Gly Ser Gly Gly Gln Gln Gly Pro Gly Gly Gln
755 760 765
Gly Pro Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly
770 775 780
Gly Tyr Gly Pro Gly Gly Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro
785 790 795 800
Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro
805 810 815
Gly Ser Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Ser
820 825 830
Gly Gln Gln Gly Pro Gly Gly Ala Gly Gln Gln Gly Pro Gly Gly Gln
835 840 845
Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala Gly
850 855 860
Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro
865 870 875 880
Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ser Ala
885 890 895
Ala Ala Gly Gly Tyr Gly Pro Gly Ser Gly Gln Gln Gly Pro Gly Gly
900 905 910
Gln Gly Pro Gly Ser Gly Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro
915 920 925
Tyr Gly Pro Gly Ser Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr
930 935 940
Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly
945 950 955 960
Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro
965 970 975
Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ala Ala Ala Ala Ala Ala
980 985 990
Ala Gly Gly Tyr Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro
995 1000 1005
Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ala Ala Ala Ala Ala
1010 1015 1020
Ala Ala Gly Gly Tyr Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln
1025 1030 1035
Gly Pro Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro
1040 1045 1050
Gly Ala Ser Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly
1055 1060 1065
Gln Gln Gly Pro Gly Gln Gln Gly Pro Ala Gln Gln Gly Pro Gly
1070 1075 1080
Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala
1085 1090 1095
Ala Gly Gly Tyr Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly
1100 1105 1110
Pro Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly
1115 1120 1125
Ala Ser Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Gln
1130 1135 1140
Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln
1145 1150 1155
Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Ala Gln
1160 1165 1170
Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala
1175 1180 1185
Ala Gly Gly Tyr Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly
1190 1195 1200
Pro Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly
1205 1210 1215
Ala Ser Ala Ala Ala Ala Ala Ala Arg Gly Tyr Gly Pro Gly Gln
1220 1225 1230
Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Leu
1235 1240 1245
Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala Ser Ala
1250 1255 1260
Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Gln Gln Gly Pro
1265 1270 1275
Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro
1280 1285 1290
Gly Gln Gln Gly Pro Gly Gly Gln Gly Pro Tyr Gly Pro Gly Ala
1295 1300 1305
Ser Ala Ala Ala Ala Ala Ala Ala Gly Gly Tyr Gly Pro Gly Gln
1310 1315 1320
Gln Gly Pro Gly Gln Gln Gly Pro Gly Gln Gln Gly Pro Gly Gly
1325 1330 1335
Gln Gly Pro Tyr Gly Pro Gly Ala Ala Ser Ala Ala Val Ser Val
1340 1345 1350
Gly Gly Tyr Gly Pro Gln Arg Ser Ser Ala Pro Val Ala Ser Ala
1355 1360 1365
Ala Ala Ser Arg Leu Ser Ser Pro Ala Ala Ser Ser Arg Val Ser
1370 1375 1380
Ser Ala Val Ser Ser Leu Val Ser Ser Gly Pro Ser Asn Pro Ala
1385 1390 1395
Ala Leu Ser Ser Thr Ile Ser Ser Ala Val Ser Gln Ile Ser Ala
1400 1405 1410
Ser Asn Pro Gly Leu Ser Gly Cys Asp Val Leu Val Gln Ala Leu
1415 1420 1425
Leu Glu Val Val Ser Ala Leu Val His Ile Leu Gly Ser Ser Ser
1430 1435 1440
Ile Gly Gln Ile Asn Tyr Gly Ala Ser Ser Gln Tyr Ala Gln Met
1445 1450 1455
Val Gly Gln Ser Val Ala Gln Ala Leu Gly
1460 1465
<210> 4
<211> 1249
<212> PRT
<213> 大腹园蛛
<400> 4
Gly Gly Gly Ser Gly Gly Gln Gly Pro Ser Gly Tyr Pro Gln Gln Gly
1 5 10 15
Pro Gly Gly Tyr Gly Pro Ser Gly Pro Gly Ala Ser Ala Ala Val Ala
20 25 30
Ala Ala Gly Gly Gln Gly Pro Ser Gly Pro Gly Pro Gln Gly Pro Ser
35 40 45
Gly Pro Gly Gly Tyr Gly Pro Ser Ser Gln Gly Pro Ser Gly Pro Gly
50 55 60
Ala Ser Ser Ala Ala Ala Ala Ala Ser Ala Ser Gly Pro Ser Gly Tyr
65 70 75 80
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Ser Gly Pro Gly Gly Tyr
85 90 95
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Ser Gly Pro Gly Ala Ser
100 105 110
Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Gln Gly Pro Ser Gly
115 120 125
Pro Gly Gly Arg Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Gly Gly
130 135 140
Pro Gly Gly Tyr Gly Pro Ser Gly Pro Gly Ser Ser Ala Ala Ala Ala
145 150 155 160
Val Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly
165 170 175
Pro Ser Gly Pro Gly Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly
180 185 190
Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly
195 200 205
Pro Gly Gly Pro Gly Ala Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser
210 215 220
Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly
225 230 235 240
Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly
245 250 255
Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly
260 265 270
Ser Ser Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly
275 280 285
Pro Gly Ser Gln Gly Pro Ser Gly Pro Ser Gly Pro Gly Gly Tyr Gly
290 295 300
Pro Ser Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser
305 310 315 320
Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly
325 330 335
Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly
340 345 350
Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly
355 360 365
Ala Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr
370 375 380
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Gly Tyr
385 390 395 400
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly
405 410 415
Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala
420 425 430
Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly
435 440 445
Pro Ser Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Ser Gly Pro Gly
450 455 460
Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr
465 470 475 480
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Gly Tyr
485 490 495
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly
500 505 510
Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala
515 520 525
Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly
530 535 540
Pro Ser Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Ser Gly Pro Gly
545 550 555 560
Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr
565 570 575
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Gly Tyr
580 585 590
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly
595 600 605
Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Ala
610 615 620
Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly
625 630 635 640
Pro Ser Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Ser Gly Pro Gly
645 650 655
Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr
660 665 670
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Gly Tyr
675 680 685
Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Gly
690 695 700
Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Ser Ser Ala Ala Glu
705 710 715 720
Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln
725 730 735
Gly Pro Ser Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro Ser Gly Pro
740 745 750
Gly Ser Ser Glu Ala Ala Ala Ala Ala Ala Ala Asn Gly Pro Gly Gly
755 760 765
Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Ser
770 775 780
Ser Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro
785 790 795 800
Gly Ser Gln Gly Pro Ser Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro
805 810 815
Ser Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly
820 825 830
Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly
835 840 845
Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly
850 855 860
Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Ser
865 870 875 880
Ser Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro
885 890 895
Gly Ser Gln Gly Pro Ser Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro
900 905 910
Ser Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser Gly
915 920 925
Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly
930 935 940
Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly
945 950 955 960
Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly Gly Pro Gly Ser
965 970 975
Ser Ala Ala Ala Ala Ala Ala Ala Ser Gly Pro Gly Gly Tyr Gly Pro
980 985 990
Gly Ser Gln Gly Pro Ser Gly Pro Ser Gly Pro Gly Gly Tyr Gly Pro
995 1000 1005
Ser Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ala Ser
1010 1015 1020
Gly Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro
1025 1030 1035
Gly Gly Pro Gly Gly Tyr Val Pro Gly Ser Gln Gly Pro Ser Gly
1040 1045 1050
Pro Gly Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Gly
1055 1060 1065
Gly Pro Gly Ser Ser Ala Ala Ala Ala Ala Ala Ala Ser Gly Ser
1070 1075 1080
Val Gly Tyr Gly Pro Gly Ser Gln Gly Pro Ser Gly Pro Ser Gly
1085 1090 1095
Pro Gly Gly Tyr Gly Pro Gly Gly Pro Gly Ala Ser Ala Ala Ala
1100 1105 1110
Ala Ala Ala Ser Gly Ala Gly Gly Tyr Gly Pro Gly Ser Gln Gly
1115 1120 1125
Pro Ser Gly Pro Gly Tyr Gln Gly Pro Ser Gly Pro Gly Ala Tyr
1130 1135 1140
Gly Pro Ser Pro Ala Ala Ser Ala Ser Val Ala Ala Ser Arg Leu
1145 1150 1155
Ser Ser Pro Ala Ala Ser Ser Arg Val Ser Ser Ala Val Ser Ser
1160 1165 1170
Leu Val Ser Ser Gly Pro Thr Asn Gly Ala Ala Val Ser Gly Ala
1175 1180 1185
Leu Asn Gly Leu Val Ser Gln Ile Ser Ala Ser Asn Pro Gly Leu
1190 1195 1200
Ser Gly Cys Asp Ala Leu Val Gln Ala Leu Leu Glu Leu Val Ser
1205 1210 1215
Ala Leu Val Ala Ile Leu Ser Ser Ala Ser Ile Gly Gln Val Asn
1220 1225 1230
Val Asn Ser Ala Ser Gln Ser Thr Gln Met Ile Ser Gln Ala Leu
1235 1240 1245
Leu
<210> 5
<211> 4953
<212> DNA
<213> 大腹园蛛
<400> 5
attcagtcga gaagacccaa aatcacaatg acttggacag ctcggcttgt gttattaact 60
ctggttgctc tctgcaccct gagtgaggtc gctcgaggtg agacgaacca cccatggaaa 120
gatgcgaata tgatgaagaa gtttatagat tctgtaagtg gatcacttaa aagctcggga 180
tgctacactg aggaagagtt gagtgccatg gatgatattg gtgacaccat gctgctaacc 240
gctgatagga tgggaagaaa aggcagagcg tctccatcgc aattagaagc tctgaacaca 300
ggttatgctg cagcattggc agagattaac tcaggtgggt caaatcaggg agaaaaagaa 360
agggctttgg acattgcttt gcgagaaggt ttatatgcgt cgacaggtgt agttgacgtt 420
aaatttctga atgaaatcaa caagttaata gaattgttta ctcagtcgaa cagattggtc 480
gacggaagtg cggcggcagt ggcaagtgca tctaatggtt acggaagtgg accacaaaca 540
ctctcatccc agggactagg atcgtcatcg atatccgttt ccgcaacagc cgcaggggga 600
ttccctcaag gaccaggggg agcaggaccg caaggaccag ttggatcaca gcaaagttct 660
tattccgtta gcgtaaacag tctcagtggt tcaccgggtg gctatggagg acaatatgga 720
ggaggacaag caggtggagc cggacaagga tatggcaatg gattaggtgg acaagatggt 780
gcctcttcag ccgcagcagc aggaggagca ggacaaggtg gtttcggtgc ttctggtgcc 840
ggacaaggat atggcgacgg attaggtgga caaggtggtg ccgcttcagc cgcagcagca 900
gcaggaggag gagcacgaca aggtggcttc ggtgcttctg gtgacggaca aggatatggc 960
gacggattag gtggacaagg tggtgccgct tcagccgcag cagcaggaga agcaggacaa 1020
gttggcttcg gtgcttctgg tgccggacaa ggatatggcg acagattagg tggacaaggt 1080
ggtgccgctt cagccgcagc aggaggagca ggacaaggtg gtttcggtgc ttatggtgcc 1140
agacaaggat atggcgacgg attaggtgga caaggaggca ccggggcctc agcagcagca 1200
gcagctggag gcgcaggaca aggtggatta ggtgcagata gtgccgtaca aggatacggt 1260
ggtggattag gtggccttgg aggatatgga caagacggag ccacggctgc cgcagcagga 1320
gcaattggta gtgcaggtgg cttaggttat ggtggagctg gaggttccag acaaggatat 1380
ggtggacaaa gaaatgcagc tggcgcaatt gatgtacaag atgaatatgg tggaatgggt 1440
caaggacgct taggcggttc aagagaattc tacaaaagag tcataattcg tactcgaggc 1500
ggttcaggtg gaactgtcgg tgagggatta ggattagggt ctggatatgg tggacaaggt 1560
agttccggat cagctgctgc agcgtcagaa tcatctgatg gatctgataa cggatatggg 1620
ccaggaatct ttggacaagg aggactcggc caaggtggat ctggcgccgc agcagcagca 1680
ggcagtggag gaggatccgg tggttttggt ggacgaggag caggtgcagg aggatacgga 1740
caaggaggat ccggctcagc tgccgcagca gctgcctcat ctagtggatc tgatagtgac 1800
ggatatggcg gaggatacaa tggacaagga ggaaccggcc aaggcggatc tggcgccgca 1860
gaagcagcag ccagtggagg acgatccggt ggttctggtg cacgaggagc aggtgcggga 1920
ggatacggac aaggaggatc cggctcagct gccgcagcag ctgcctcatc tagtggatct 1980
ggtagtgacg gatatggcgg aggatacaat ggacaaggag gacccggcca aggcggatct 2040
ggcgccgcag cagcagcagc cagtggagga ggatccggtg gttctggtgg acgaagagca 2100
ggtgcaggag gatacggaca aggaggatcc ggctcagctg ccgcagctgc ctcatccagt 2160
ggatctggtg gtgacggata tggcggagga tacaatggac aaggaggacc cggccaaggc 2220
ggatctggcg ccgcagcagc agcagccagt ggaggaggat ccggtggttt tggtggacga 2280
ggagcaggtg caggaggata cggacaagga ggatccggct cagctgccgc agcagctgcc 2340
tcatctagtg gatctggtag tgacggatat ggcggaggat acaatggaca aggaggaacc 2400
ggccaaggcg gatctggcgc cgcagcagca gcagccggtg gaggaggatc cggtggttct 2460
ggtggacgag gagcaggtgc aggaggatac ggacaaggag gatccggctc agctgccgca 2520
gctgcctcat tcagtggatc tggtggtgac ggatatggcg gaggatacaa tggacaagga 2580
ggacccggcc aaggcggatc tggcgccgca gcagcagcag ccagtggagg agaatccggt 2640
ggttctggtg gacgaggagc aggtgcagga agatacggac aaggaggatc cggctcagct 2700
gccgcagcag ctgcctcatc cagtggatct ggtagtgacg gatatggcgg aggatacaat 2760
ggacaaggag gacccggcca aggcggatct agcgccgcag cagcagcagg cagtggagga 2820
ggatccggtg gttttggtgg acgaggagca ggtgcaggag gatacggaca aggaggatcc 2880
ggctcagctg ccgcagctgc ctcatccagt ggatctggtg gtgacggata tggcggagga 2940
tacaatggac aaggaggacc cggccaaggc ggatctggcg ccgcagcagc agcagccagt 3000
ggaggaggat ccggtggttt tggtggacga ggagcaggtg caggaggata cggacaagga 3060
ggatccggca cagctgccgc agcagctgcc tcatctagtg gttctggtag tgacggatat 3120
ggcggaggat acaatggaca aggaggaacc ggccaaggcg gatctggcgc cgcagcagca 3180
gcagccggtg gaggaggatc cggtggttct ggtggacgag gagcaggtgc aggaggatac 3240
ggacaaggag gatccggctc agctgccgca gctgcctcat ccagtggatc tggtggtgac 3300
ggatatggcg gaggatacaa tggacaagga ggacccggcc aaggcggatc tggcgccgca 3360
gcagcagcag ccagtggagg aggatccggt ggttctggtg gacgaggagc aggtgcagga 3420
agatacggac aaggaggatc cggctcagct gccgcagcag ctgcctcatc cattggatct 3480
ggtagtgacg gatatggcgg aggatacaat ggacaaggag gacccggcca aggcggatct 3540
ggcgccgcag cagcagcagc cagtggagga ggatccggtg gttctggtgg acgaggagca 3600
ggtgcaggaa gatacggacg aggaggatcc ggctcagctg ccgcagcagc tgcctcatcc 3660
agtggatctg gtagtgacgg atatggcgga ggatacaatg gacaaggagg acccggccaa 3720
ggcggatcta gcgccgcagc agcagcaggc agtggaggag gatccggtgg ttttggtgga 3780
cgaggagcag gtgcaggagg atacggacaa ggaggatccg gctcagctgc ctcagctgcc 3840
tcatccagtg gatctggtgg tgacggatat ggcggaggat acaatggaca aggaggaccc 3900
ggccaaggcg gatctggcgc cgcagcagca gcagccagtg gaggacgatc cggtggttct 3960
ggtggacgag gagcaggtgc aggaggatac ggacaaggag gatccggttc agctgccgca 4020
gcagctgcct catccagtgg atctggtagt gacggatatg gcggaggata caatggacaa 4080
ggaggacccg gccaaggcgg atctggcgcc gcagcagcag cagcagccag tggaggagaa 4140
tccggtggtt ctggtggacg aggagcaggt tcaggaggat acggactagg aggatccggc 4200
tcagctgcgg cagcagctgc ctcatctagt ggatctggta gtgacagata tggcggagga 4260
tacaaaggac aaggaggatc cggctcagct gccgcggcag ctgcctcatc taatggatct 4320
ggtagtgacg gatatggcgg aggatacaat ggacaaggag gacccggcca aggcggctct 4380
gtcgccgcag cagcagcagc cagtagagga ggatccggtg gttttggtgg acgaggagta 4440
agtccaggag gatacggaaa aggttctacc ggatcatcgt cggcagcagc tgcgtcattt 4500
gatgcatctg gtagcgacgg atatggccgt gaattcggtg gacaaggagg atctggtcaa 4560
aacggtgctt ctgccgctac atcctcatcc agcggaggag gattcggcgg ttatggtgca 4620
ggtcaaggag tgtacggagg tggcctacaa gagcctgcag ctgcgtcggc agctgtagct 4680
tcttctgctg cttcccgttt gtcatctccc tcttcccggt caagagtctc gtctgctgtc 4740
tctatcttct tagataatgg cctacgatct ccagaggcat tttctagtgt ttataacagc 4800
tttgtctctg atataatatc atccaatcct aatctatctg gatgtgaagt ccttgttcaa 4860
gtcttactag aaatcttggc ggcaactatt agtggacttt ccggtttaga ttctaattcg 4920
tcgatgaatg ttgttaaatc ttttgttagt taa 4953
<210> 6
<211> 4764
<212> DNA
<213> 大腹园蛛
<400> 6
caacaaggac ctggaggtca aggaccatat ggacctggtg catccgccgc cgcagcagcc 60
gctgctggag gatatggacc tggatctgga caacaaggac ctggacagca aggacccgga 120
ggtcaaggtc cttatggacc tggttcagcc gccgccgctg cagcagcagc aggaggttat 180
ggacccggat ctggacaaca aggacctgga ggagctggac aacaaggacc tggaggtcaa 240
ggaccatatg gacctggtgc atccgccgcc gcagcagcag cagctggagg atatggaccc 300
ggaggtggac aacaaggacc tggaggtcaa ggcacaggag gagccggtca acaaggacca 360
ggaggccagg gaccatacgg acctggatca gccgccgccg ctgcagcagc agcaggaggt 420
tatggacccg gatctggaca acaaggacct ggaggagccg gtcaacaagg acctggaggt 480
caaggaccat atggacctgg tgcatccgcc gcagcagcag tcgctgctgg aggatatgga 540
cctggatctg gacaacaagg acctggacag caaggtcccg gaggtcaagg tccttatgga 600
cctggttcag ccgccgccgc tgcagcagca gcaggaggtt atggacccgg atctggacaa 660
caaggacctg gaggagctgg acaacaagga cctggaggtc aaggtccata tggacctggt 720
gcatccgccg ccgcagcagc agcagctgga ggatatggac ccgggtctgg tcaacaagga 780
cctggaggtc aaggctcagg aggagccggt caacaaggac caggaggcca gggaccatac 840
ggacctggat cagccgccgc cgctgcagca gcagcaggag gttatggacc cggatctgga 900
caacaaggac ctggaggagc cggtcaacaa ggacctggag gtcaaggacc atatggacct 960
ggtgcatccg ccgcagtagc agccgctgct ggaggatatg gacctggatc tggacaacaa 1020
ggacctggac agcaaggacc cggaggtcaa ggtccttatg gacctggttc agccgccgcc 1080
gctgcagcag ctgcaggagg ttatggaccc ggatctggac aacaaggacc tggaggtcaa 1140
ggatcatatg gaactggtgc atccgcctcc gcagcagcag cagctggagg atatggaccc 1200
ggaggtggac aacaaggacc tggaggtcaa ggcacaggag gagccggtca acaaggacca 1260
ggaggccagg gaccatacgg acctggatca gccgccgccg ctgcagcagc agcaggagga 1320
tatggaccgg gacctggaca acaaggacct ggaggagccg gtcaacaagg acctggaggt 1380
caaggaccat atggacctgc tgcatccgcc gccgcagcag tcgctgctgg aggatatgga 1440
cctggatctg gacaacaagg acctggacag caaggtcccg gaggtcaagg tccttatgga 1500
cctggttcag ccgccgccgc tgcagcagca gcaggaggtt atggacccgg atctggacaa 1560
caaggacctg gaggagctgg acaacaagga cctggaggtc aaggtccata tggacctggt 1620
gcatccgccg ccgccgcagc agcagctgga ggatatggac ccgggtctgg tcaacaagga 1680
cctggaggtc aaggctcagg aggagccggt caacaaggac caggaggcca gggaccatac 1740
ggacctggat cagccgccgc cgctgcagca gcagcaggag gttatggact cggatctgga 1800
caacaaggac ctggaggagc cggtcaacaa ggacctggag gtcaaggacc atatggacct 1860
ggtgcatccg ccgcagcagc agccgctgct ggaggatatg gacctggatc tggacaacaa 1920
ggacctggac agcaaggacc cggaggtcaa ggtccttatg gacctggttc agccgccgcc 1980
gctgcagcag ctgcaggagg ttatggaccc ggatctggac aacaaggacc tggaggtcaa 2040
ggatcatatg gaactggtgc atccgcctcc gcagcagcag cagctggagg atatggaccc 2100
ggaggtggac aacaaggacc tggaggtcaa ggctcaggag gagccggtca acaaggacca 2160
ggaggccagg gaccatacgg acctggatca gccgccgccg ctgcagcagc agcaggaggt 2220
tatggaccgg gatctggaca acaaggacct ggaggagccg gtcaacaagg accgggaggt 2280
caaggacctt atggacctgg tggagccgcc gcagcagcag cagctggagg atatggaccc 2340
ggaggtcaag gaccaggaag tgggggacaa caaggtccta gtggtcaggg accatatgga 2400
cctggttctg ccgccgccgc tgcagcagca gcaggaggtt atggacccgg atctggacaa 2460
caaggacctg gaggagccgg tcaacaagga actggaggtc aaggaccgta tggacctggt 2520
gtatccgccg ccgcctcagc agcagctgga ggatatggac ccggaggtgg acaacaagga 2580
tctggaggtc aaggctcagg aggagccggt caacaaggac caggaggcca gggaccatac 2640
ggacctggat cagctgccgc cgctgcagca gcagcaggag gttatggacc gggatctgga 2700
caacaaggac ctggaggagc cggtcaacaa ggaccgggag gtcaaggacc ttatggacct 2760
ggtggagccg ccgcagcagc agcagctgga ggatatggac ccggaggtca aggaccagga 2820
agtggtggac aacaaggtcc tggtggtcag ggaccatatg gacctggttc tgccgccgcc 2880
gctgcagcag cagcaggagg ttatggaccc ggatctggac aacaaggacc tggaggagcc 2940
ggtcaacaag gacctggagg tcaaggacca tatggacctg gtgcatccgc cgcagcagca 3000
gctgctgctg gaggatatgg acctggatct ggacaacaag gacctggaca gcaaggaccc 3060
ggaggtcaag gtccttatgg acctggttca gccgccgccg ctgcagcagc agtaggaggt 3120
tatggacccg gatctggaca acaaggacct ggaggagccg gtcaacaagg acctggaggt 3180
caaggaccat atggacctgg cgcggcagcc gcagcagcag ccgcaggtgg atatggacct 3240
ggtgcaggag gaccacaagg acctggagga gctggaccac aaggaccatc aggcccaggt 3300
caacaaggac ctggaggtca aggaccttat ggacctggtg ccgcagccgc agcagcagct 3360
gccgcaggtg gttatggacc acgaggtggt gcaggacctg gaagtgtcgg acaacaagga 3420
tcttcaggtg caggacaata tggacctggt gctgcagccg cagcagcagc tgctggaggt 3480
tatggacccg gatctggaca acaaggacct ggacagcaag gacccggagg tcaaggacct 3540
tatggacctg gttcagccgc cgccgctgca gcagcagcag gaggttatgg acccggatct 3600
ggacaacaag gacctggagg agctggacaa caaggacctg gaggtcaagg accatatgga 3660
cctggtgcat ccgccgccgc cgcagcagca gcagctggag gatatggacc cggaggtgga 3720
caacaaggac ctggaggtca aggcacagga ggagccggtc aacaaggacc aggaggccag 3780
gtaccatacg gacctggatc agccgccgca gctgcagcag cagcaggagg ttatggaccc 3840
ggatctggac aacaaggacc tggaggagct ggacaacaag gacctggagg tcaaggatca 3900
tatggacctg gtgcatccgc agcagcagca gcagccgctg gaggttatgg acctggacaa 3960
caaggaccta gacaacaagg acccggtcag caaggacccg gacaacaagg acccggaggt 4020
caaggaccat atggacctgg tgcatccgca gcagcagcag cagccgctgg aggttatgga 4080
cctggacaac aaggacctag acaacaagga cccggtcagc aaggacccgg acaacaagga 4140
cccggaggtc aaggaccata tggacctggt gcatccgcag cagcagccgc tggaggttat 4200
ggacctggac aacaaggacc cggacagcaa ggacctggac aacaaggacc cggaggccaa 4260
ggaccatatg gacctggtgc atccgcagca gcagcagcag cctctggagg ttatggacct 4320
ggacaacaag gacccggaca gcaaggacgc ggacaacaag gacccggagg tcaaggacca 4380
tacggacctg gtgcggcttc cgctgctgta tctgttggag gatatggacc acaaagatcc 4440
tctgctcctg ttgcatccgc agcagcatct cgcctgtctt ctccagcggc ctcttctaga 4500
gtttcgtcag cggtgtcatc tttggtgtcc agtggtcctt ctaaccctgc cgcactttct 4560
agtaccatca gtagcgctgt ttcccaaatc agtgcaagta atcctggtct ttctggttgc 4620
gatgtacttg tgcaagcatt actggaagtt gtatcggccc tcgtccatat tcttggctct 4680
tctagtatcg ggcaaattaa ctatggcgcc tcctctcagt atgcgcaaat ggtaggccaa 4740
tctgtagctc aagctcttgg ttaa 4764
<210> 7
<211> 4407
<212> DNA
<213> 大腹园蛛
<400> 7
caacaaggac ctggaggtca aggcccagga ggagccggtc aacaaggacc aggaggagcc 60
ggtcaacaag gaccaggagg ccagggacca tacggacctg gatcagccgc cgcagctgca 120
gcagctggag gatatggacc cggaggtgga caacaaggac ctggaggtca aggcccagga 180
ggagccggtc aacaaggacc aggaggccag ggaccatacg gacctggatc agcctccgca 240
gctgcagcag cagcaggagg ttatggaccc ggatctggac aacaaggacc tggaggagct 300
ggacaacaag gacctggagg tcaaggacca tatggacctg gtgcatccgc cgccgcagca 360
gcagcagctg gaggatatgg acccggaggt ggacaacaag gaccaggagg tcaaggccca 420
ggaggagccg gtcaacaagg accaggagga gccggtcaac aaggacctgg aggccaggga 480
ccatacggac ctggatcagc cgccgcagct gcagcagcag caggaggtta tggacccgga 540
tctggacaac aaggacctgg aggagctgga caacaaggac ctggaggagc tggacaacaa 600
ggacctggag gagctggaca acaaggacct ggaggtcaag gaccatatgg acctggtgca 660
tccgccgccg cagcagcagc agctggagga tatggacccg gaggtggaca acaaggacct 720
ggaggtcaag gcccaggagg agccggtcaa caaggaccag gaggagccgg tcaacaagga 780
ccaggaggcc agggaccata cggacctgga tcagccgccg cagctgcagc agcagcagga 840
ggttatggac ccggatctgg acaacaagga cctggaggtg aaggaccata tggacctggt 900
gcatccgccg ccgcagcagc agcagctgga ggatatagac ccggaggtgg acaacaagga 960
cctggaggtc aaggcccagg aggagccggt caacaaggac caggaggagc cggtcaacaa 1020
ggaccaggag gccagggacc atacggacct ggatcagccg cggcagctgc agcagcagca 1080
ggaggttatg gacccggatc tggacaacaa ggacccggac agcaaggacc cggaggtcaa 1140
ggaccatatg gacctggtgc agcagcagca ggaggatatg gacccggatc tggacaacaa 1200
ggaactggac agcaaggacc cggaggtcaa ggaccttatg gacctggttc agccgccgcc 1260
gctgcagcag cagcaggagg ttatggaccc ggatctggac aacaaggacc tggaggagct 1320
ggacaacaag gacctggagg tcaaggacca tatggacctg gtgcatccgc cgccgcagca 1380
gcagcagctg gaggatatgg acccggaggt ggacaacaag gacctggagg tcaaggccca 1440
ggaggagccg gtcaacaagg accaggaggc cagggaccat acggacctgg atcagccgcc 1500
gccgctgcag cagcagcagg aggttatgga cccggatctg gacaacaagg acctggagga 1560
gccggtcaac aaggacctgg aagtcaagga ccatatggac ctggtgcatc cgccgccgca 1620
gcagcggcag ctggaggata tggacccgga ggtggacaac aaggacctgg aggtcaaggc 1680
ccaggaggag ccggtcaaca aggaccagga ggagccggtc aacaaggacc aggaggccag 1740
ggaccatacg gacctggatc agccgcggca gctgcagcag cagcaggagg ttatggaccc 1800
ggatctggac aacaaggacc cggacagcaa ggacccggag gtcaaggacc atatggacct 1860
ggtgcagcag cagcaggagg atatggaccc ggatctggac aacaaggaac tggaggtcaa 1920
ggcccaggag gagccggtca acaaggacca ggaggccagg gaccttacgg acctggatca 1980
gccgccgccg ctgcagcagg agcaggaggt tatggacccg gatctggaca acaaggacct 2040
ggaggagccg gtcaacaagg acctggaagt caaggaccat atggacctgg tgcatccgcc 2100
gcagcagcag ccgctgctgg aggatatgga cctggatctg gacaacaagg acctggacag 2160
caaggacctg gaggtcaagg actttatgga cctggtggag ccgccgcagc agcagcagct 2220
ggaggatatg gacccggatc tggtcaacaa ggacctggag gtcaaggacc aggaagtggt 2280
ggacaacaag gtcctggtgg tcagggacca tatggacctg gctcagccgc cgccgctgca 2340
gcagcagctg gaggatatgg acccggaggt ggacaacaag gacctggagg tcaaggccca 2400
ggaggagccg gtcaacaagg accaggaggc cagggaccat acggacctgg atcagccgcc 2460
gcagcagcag cagcaggagg ttatggaccc ggatctggac aacaaggacc tggaggagcc 2520
ggtcaacaag gacctggagg tcaaggacca tatggacctg gtgcatccgc cgcagcagca 2580
gccgctgctg gaggatatgg acctggatct ggacaacaag gacctggaca gcaaggaccc 2640
ggaggtcaag gaccatatgg acctggtgca tctgccgcag catcagccgc tgctggagga 2700
tatggacccg gatctggtca acaaggacct ggaggtcaag gaccaggaag tggtggacaa 2760
caaggtcctg gtggtcaagg accatatgga cctggttcag ccgccgccgc tgcagcagca 2820
gcaggaggtt atggacctgg acaacaagga cccggacagc aaggacccgg acaacaagga 2880
cctggacaac aaggacccgg acagcaagga cccggacaac aaggacccgg aggtcaagga 2940
ccatacggac ctggtgcagc cgccgcagca gcagccgccg gaggttatgg acctggacaa 3000
caaggacccg gacaacaagg acccggaggt caaggaccat acggacctgg tgcagccgcc 3060
gcagcagcag ccgctggagg ttatggacct ggacaacaag gacccggaca gcaaggaccc 3120
ggacaacaag gacccggagg tcaaggacca tatggacctg gtgcatccgc agcagcagca 3180
gccgctggag gttatggacc tggacaacaa ggacccggac agcaaggacc cgcacaacaa 3240
ggacccggag gtcaaggacc atatgggcct ggtgcatccg cagcagcagc agccgctgga 3300
ggttatggac ctggacaaca aggacccgga cagcaaggac ccggacaaca aggacccgga 3360
ggtcaaggac catatggacc tggtgcatcc gcagcagcag cagccgctgg aggttatgga 3420
cctggacaac aaggacccgg acaacaagga cccggacaac aaggacccgg acaacaagga 3480
cccggacaac aaggacccgg acaacaagga cctgcacaac aaggaccata tggacctggt 3540
gcatccgcag cagcagcagc agccgctgga ggttatggac ctggacaaca aggacccgga 3600
cagcaaggac ccggacaaca aggacccgga ggtcaaggac catatggacc tggtgcatcc 3660
gcagcagcag cagccgctag aggttatgga cctggacaac aaggacctgg acaacaagga 3720
cccggtcagc aaggacccgg actacaagga cctggaggtc aaggaccata tggacctggt 3780
gcatccgcag cagcagcagc cgctggaggt tatggacctg gacaacaagg acctggacaa 3840
caaggacctg gacaacaagg acccggacag caaggacccg gacaacaagg acccggaggt 3900
caaggaccat atggacctgg tgcatccgca gcagcagcag cagccgctgg aggttatgga 3960
cctggacaac aaggacccgg acagcaagga cccggacaac aaggacccgg aggtcaagga 4020
ccatacggac ctggtgcggc ttccgctgct gtatctgttg gaggatatgg accacaaaga 4080
tcctctgctc ctgttgcatc cgcagcagca tctcgcctgt cttctccagc ggcctcttct 4140
agagtttcgt cagcggtgtc atctttggtg tccagtggtc cttctaaccc tgccgcactt 4200
tctagtacca tcagtagcgc tgtttcccaa atcagtgcaa gtaatcctgg tctttctggt 4260
tgcgatgtac ttgtgcaagc attactggaa gttgtatcgg ccctcgtcca tattcttggc 4320
tcttctagta tcgggcaaat taactatggc gcctcctctc agtatgcgca aatggtaggc 4380
caatctgtag ctcaagctct tggttaa 4407
<210> 8
<211> 3750
<212> DNA
<213> 大腹园蛛
<400> 8
ggaggtggat ccggaggtca aggaccatct ggatatccac aacaaggacc tggaggttac 60
ggaccatctg gacctggtgc atcagctgcg gtcgcagcag ctggaggtca aggaccttct 120
ggaccgggac cacaagggcc atccggacca ggaggttacg gcccaagtag ccaaggacca 180
tctggaccgg gagcatcctc agcagcagca gccgcttcag caagtggacc tagcgggtat 240
ggacctggaa gccaaggacc atctggacca agcggacctg gaggatatgg tccaggaagt 300
caaggaccat ctggacctag tggacctgga gcatccgcag cagctgctgc agcaagtgga 360
ccaggaggcc aagggccatc tggacccgga ggacgtggag gatatggtcc tggaagccaa 420
ggacctggtg gacctggagg atatggacct agtggacccg gatcttcagc agcagcagca 480
gtcgctgcag caagtggacc tggaggatat ggacctggaa gccaaggacc atctggacct 540
ggtggacccg gaggatatgg tcccggaagc caaggaccat ctggacctgg aggatacgga 600
cctggaagcc aaggaccatc tggacctggt ggacccggag cttcagcagc agcagccgcc 660
gctgcagcaa gtggacctgg aggatatgga cctggaagcc aaggaccatc tggacctggt 720
ggacccggag gatatggtcc cggaagccaa ggaccatctg gacctggagg atacggacct 780
ggaagccaag gaccatctgg acctggtgga cccggatctt cagcagcagc agccgctgca 840
gcaagtggac ccggtggata cggacctgga agccaaggac catctggacc tagtggacct 900
ggaggatatg gacctagtgg acccggatct tcagcagcag cagccgccgc tgcagcaagt 960
ggacctggag gatatggacc tggaagccaa ggaccatctg gacctggtgg acccggagga 1020
tatggtcccg gaagccaagg accatctgga cctggaggat acggacctgg aagccaagga 1080
ccatctggac ctggtggacc cggagcttca gcagcagcag ccgccgctgc agcaagtgga 1140
cctggaggat atggacctgg aagccaagga ccatctggac ctggtggacc cggaggatat 1200
ggtcccggaa gccaaggacc atctggacct ggaggatacg gacctggaag ccaaggacca 1260
tctggacctg gtggacccgg atcttcagca gcagcagccg ctgcagcaag tggacccggt 1320
ggatacggac ctggaagcca aggaccatct ggacctagtg gacctggagg atatggacct 1380
agtggacccg gatcttcagc agcagcagcc gccgctgcag caagtggacc tggaggatat 1440
ggacctggaa gccaaggacc atctggacct ggtggacccg gaggatatgg tcccggaagc 1500
caaggaccat ctggacctgg aggatacgga cctggaagcc aaggaccatc tggacctggt 1560
ggacccggat cttcagcagc agcagccgct gcagcaagtg gacccggtgg atacggacct 1620
ggaagccaag gaccatctgg acctagtgga cctggaggat atggacctag tggacccgga 1680
tcttcagcag cagcagctgc cgctgcagca agtggacctg gaggatatgg acctggaagc 1740
caaggaccat ctggacctgg tggacccgga ggatatggtc ccggaagcca aggaccatct 1800
ggacctggag gatacggacc tggaagccaa ggaccatctg gacctggtgg acccggatct 1860
tcagcagcag cagccgctgc agcaagtgga cccggtggat acggacctgg aagccaagga 1920
ccatctggac ctagtggacc tggaggatat ggacctagtg gacccggatc ttcagcagca 1980
gcagctgccg ctgcagcaag tggacctgga ggatatggac ctggaagtca aggaccatct 2040
ggacctggtg gacccggagg atatggtccc ggaagccaag gaccatctgg acctggagga 2100
tacggacctg gaagccaagg accatctgga cctggtggac ccggatcttc agcagcagaa 2160
gcagccgctg cagcaagtgg acccggtgga tacggacctg gaagccaagg accatctgga 2220
cctagtggac ctggaggata tggacctagt ggacccggat cttcagaagc agcagctgcc 2280
gctgcagcaa atggacctgg aggatacgga cctggaagcc aaggaccatc tggacctggt 2340
ggacccggat cttcagcagc agcagccgct gcagcaagtg gacccggtgg atacggacct 2400
ggaagccaag gaccatctgg acctagtgga cctggaggat atggacctag tggacccgga 2460
tcttcagcag cagcagctgc cgctgcagca agtggacctg gaggatatgg acctggaagc 2520
caaggaccat ctggacctgg tggacccgga ggatatggtc ccggaagcca aggaccatct 2580
ggacctggag gatacggacc tggaagccaa ggaccatctg gacctggtgg acccggatct 2640
tcagcagcag cagccgctgc agcaagtgga cccggtggat acggacctgg aagccaagga 2700
ccatctggac ctagtggacc tggaggatat ggacctagtg gacccggatc ttcagcagca 2760
gcagctgccg ctgcagcaag tggacctgga ggatatggac ctggaagcca aggaccatct 2820
ggacctggtg gacccggagg atatggtccc ggaagccaag gaccatctgg acctggagga 2880
tacggacctg gaagccaagg accatctgga cctggtggac ccggatcttc agcagcagca 2940
gccgctgcag caagtggacc cggtggatac ggacctggaa gccaaggacc atctggacct 3000
agtggacctg gaggatatgg acctagtgga cccggatctt cagcagcagc agccgccgct 3060
gcagcaagtg gacctggagg atatggacct ggaagccaag gaccatctgg acctggtgga 3120
cccggaggat atgttcccgg aagccaagga ccatctggac ctggaggata cggacctgga 3180
agccaaggac catctggacc tggtggaccc ggatcttcag cagcagcagc cgctgcagca 3240
agtggatccg ttggatacgg acctggaagc caaggaccat ctggacctag tggacctgga 3300
ggatatggac ctggtggacc cggagcttca gcagcagccg ctgcagcaag tggagctgga 3360
ggatatggac ccggaagcca aggaccatct ggacctggat atcaaggccc tagtggtcct 3420
ggagcatatg gcccatctcc tgctgcttca gcatctgttg cagcctctcg tttatcttca 3480
cctgcagcct cgtctagagt gtcttccgct gtatcgtctt tagtttctag cggacctacg 3540
aatggtgctg ctgtttctgg agctttgaat ggtttggtat ctcagattag tgcaagtaat 3600
ccaggcttat cgggatgtga cgctcttgtg caggcattat tggaattggt gtctgctctt 3660
gtagcaattc tttcatctgc aagtattggc caagtcaacg tcaactctgc tagtcagtcg 3720
actcaaatga ttagccaggc tcttttataa 3750
Claims (9)
1.一种蛋白质，其氨基酸序列为SEQ ID NO:1。
2.一种蛋白质，其特征在于，其由蛋白质A组成，其中编码蛋白质A的核苷酸序列如SEQID NO:5所示。
3.编码权利要求1所述蛋白质的核酸。
4.根据权利要求3所述核酸，其核苷酸序列为SEQ ID NO:5所示。
5.含有权利要求3或4所述的核酸的重组载体。
6.用权利要求5所述的重组载体转化或转染的宿主细胞。
7.权利要求1所述蛋白质的制备方法，其特征在于，其包括用权利要求6所述的宿主细胞表达权利要求1所述的蛋白质，然后分离权利要求1所述的蛋白质。
8.SEQ ID NO:1所示的蛋白质在人造材料中的应用。
9.一种人造材料，其特征在于，其含有SEQ ID NO:1所示的蛋白质。
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