CN110257225A - Isolating device and instrument for extracting nucleic acid - Google Patents
Isolating device and instrument for extracting nucleic acid Download PDFInfo
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- CN110257225A CN110257225A CN201910723629.1A CN201910723629A CN110257225A CN 110257225 A CN110257225 A CN 110257225A CN 201910723629 A CN201910723629 A CN 201910723629A CN 110257225 A CN110257225 A CN 110257225A
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- isolating device
- shell
- nucleic acid
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 62
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 62
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 62
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 108
- 230000007246 mechanism Effects 0.000 claims abstract description 60
- 238000000605 extraction Methods 0.000 claims abstract description 46
- 238000007789 sealing Methods 0.000 claims description 35
- 238000005192 partition Methods 0.000 claims description 11
- 206010011409 Cross infection Diseases 0.000 abstract description 4
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 4
- 239000000443 aerosol Substances 0.000 description 9
- 238000010586 diagram Methods 0.000 description 9
- 239000000126 substance Substances 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000004308 accommodation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 208000005252 hepatitis A Diseases 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
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- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of isolating device and instrument for extracting nucleic acid, are related to nucleic acid extraction technical field, and the isolating device includes shell, include two or more mutually isolated receiving areas in the shell, the receiving area is for accommodating at least one reagent housing unit；The shell corresponds to each receiving area and is respectively arranged with the limit hole being connected to the receiving area, the length direction of the limit hole is identical as the length direction of the reagent housing unit, there are mobile space between the limit hole and the reagent housing unit, the mobile space is used for the active section movement for nucleic acid extraction mechanism.Multiple reagent housing units in reagent holding mechanism are isolated by multiple receiving areas for the isolating device, to reduce its cross-infection possibility.
Description
Technical field
The present invention relates to nucleic acid extraction fields, in particular to a kind of isolating device and instrument for extracting nucleic acid.
Background technique
Nucleic acid is the basic object of molecular biology research, and nucleic acid extraction step is the basic link in molecular diagnosis.Cause
This, the quality of the nucleic acid extracted during nucleic acid extraction influences the result of molecular diagnosis very big.
In the prior art, nucleic acid extraction operation is carried out usually using instrument for extracting nucleic acid.Instrument for extracting nucleic acid includes shell, shell
Inside forms closed chamber, is provided with reagent holding mechanism and nucleic acid extraction mechanism in closed chamber, reagent holding mechanism has
Multiple holding tanks, nucleic acid extraction mechanism are sequentially inserted into multiple holding tanks for being placed with different reagents, to carry out nucleic acid extraction operation.
To improve nucleic acid extraction efficiency, the nucleic acid extraction operation of multisample can be carried out in shell, accordingly, nucleic acid extraction mechanism includes
Multiple extracting pieces, multiple extracting pieces extract operation simultaneously.
But in nucleic acid extraction operation process, there may be the substances such as aerosol for reaction, and aerosol is generated at one,
Aerosol will flow everywhere inside housings, and multiple extracting pieces in nucleic acid extraction mechanism will receive aerosol influence, so that
During subsequent detection, the substance that multiple extracting pieces in nucleic acid extraction mechanism extract can not obtain accurate detection knot
The viruses such as fruit, such as hepatitis A, hepatitis B can not be detected.
Summary of the invention
The purpose of the present invention is to provide a kind of isolating device and instrument for extracting nucleic acid, can mitigate core to a certain extent
Cross contamination is generated in sour extraction process.
The embodiment of the present invention is achieved in that
A kind of isolating device, including shell include two or more mutually isolated receiving areas in the shell,
The receiving area is for accommodating at least one reagent housing unit；
The shell corresponds to each receiving area and is respectively arranged with the limit hole being connected to the receiving area, the limit hole
Length direction it is identical as the length direction of the reagent housing unit, exist between the limit hole and the reagent receiving member
Mobile space, the mobile space are used for the active section movement for nucleic acid extraction mechanism.
In preferred embodiments of the present invention, the shell includes ontology and upper cover, and the upper cover is covered on the ontology,
Partition is provided in the ontology, the ontology is divided into multiple receiving areas by the partition, and the limit hole is set to
The upper cover, the ontology are detachably connected with the upper cover.
In preferred embodiments of the present invention, elastic sealing gasket, the bullet are provided between the ontology and the upper cover
Property gasket correspond to the region of the limit hole and be provided with the first designated port, the length direction of first designated port and the limit
The length direction in hole is identical.
In preferred embodiments of the present invention, it is provided with multiple second designated ports on the elastic sealing gasket, described second stroke
Mouth intersects with first designated port, and each second designated port is arranged along the length direction interval of first designated port.
In preferred embodiments of the present invention, the elastic sealing gasket includes pad, and the limit is corresponded on the pad
The region in hole is provided with male part, and the male part protrudes into the limit hole, and first designated port is set to the male part.
In preferred embodiments of the present invention, support plate is provided between the ontology and the elastic sealing gasket, it is described
The region opposite with the limit hole is provided with long hole in support plate.
In preferred embodiments of the present invention, it is provided with limit inserted block on the partition, the elastic sealing gasket and described
Be provided with limit socket on shell, the limit inserted block sequentially pass through protrude into after the limit socket of the elastic sealing gasket it is described
The limit socket of shell.
In preferred embodiments of the present invention, the side opposite with the limit hole is provided with reagent wells on the shell,
The reagent wells are used to reach the hull outside for the partial region of the reagent housing unit.
In preferred embodiments of the present invention, the shell is connected by multiple disassembling boxes and is formed, and each disassembling box can
It is detachably connected, each disassembling box has a receiving area.
A kind of instrument for extracting nucleic acid comprising shell, the inner cavity of the shell is provided with nucleic acid extraction mechanism, reagent accommodates machine
Structure and isolating device as described in the above technical scheme, the reagent holding mechanism include multiple reagent housing units, the examination
Agent housing unit is provided with multiple holding tanks, at least one reagent housing unit the opening of each holding tank be respectively positioned on it is described every
From on the inside of device, the limit hole that the nucleic acid extraction mechanism can pass through the isolating device protrudes into the holding tank.
The beneficial effect of the embodiment of the present invention is:
Isolating device provided by the present application can be applied in instrument for extracting nucleic acid, and reagent receiving is provided in instrument for extracting nucleic acid
Mechanism, reagent holding mechanism include multiple reagent housing units, and reagent housing unit is provided with multiple holding tanks.Isolating device will
Multiple reagent housing units in reagent holding mechanism in instrument for extracting nucleic acid are isolated, for example, can each receiving area only
A reagent housing unit is placed, or two or more reagent housing units can also be placed in each receiving area.Into
During row nucleic acid extraction, nucleic acid extraction mechanism can protrude into shell by limit hole, to be inserted into a reagent housing unit
Some holding tank, and reacted with the reagent of holding tank, after completing this secondary response, nucleic acid extraction mechanism removes the holding tank, and
Another holding tank being moved in the reagent housing unit carries out next step reaction.
Since the region (active section) in nucleic acid extraction mechanism moving process, contacted with reagent is always positioned in shell
Mobile space, therefore, on the one hand, multiple reagent housing units are isolated for receiving area so that even if portion of reagent accommodate
There are the substances such as aerosol at unit, aerosol will not wave to other receiving areas, so as to reduce and other receivings
There is cross-infection situation in reagent housing unit in area, and large area infection conditions is avoided to occur；On the other hand, since nucleic acid mentions
It takes the active section of mechanism in moving process, is always positioned at the mobile space of corresponding accommodating chamber, therefore even if the accommodation space
It is interior there are substances such as aerosols, the substances such as aerosol will not be adsorbed in reagent and be taken to outside shell by nucleic acid extraction mechanism
Side avoids so as to which aerosol is effectively sealed in the accommodating chamber so that reagent infects in other accommodating chambers.
To sum up, reagent holding mechanism can be isolated in multiple receiving areas using above-mentioned isolating device, difference is alleviated or avoided
Cross-infection in receiving area between substance.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the assembling schematic diagram one of isolating device provided in an embodiment of the present invention and reagent holding mechanism；
Fig. 2 is the assembling schematic diagram two of isolating device provided in an embodiment of the present invention and reagent holding mechanism；
Fig. 3 is the cross-sectional view of the assembly of isolating device provided in an embodiment of the present invention and reagent holding mechanism；
Fig. 4 is the assembly signal of ontology, support plate and reagent holding mechanism in isolating device provided in an embodiment of the present invention
Figure；
Fig. 5 is the assembling schematic diagram one of ontology and reagent holding mechanism in isolating device provided in an embodiment of the present invention；
Fig. 6 is the assembling schematic diagram two of ontology and reagent holding mechanism in isolating device provided in an embodiment of the present invention；
Fig. 7 is the structural schematic diagram one of ontology in isolating device provided in an embodiment of the present invention；
Fig. 8 is the structural schematic diagram two of ontology in isolating device provided in an embodiment of the present invention；
Fig. 9 is the structural schematic diagram one of elastic sealing gasket in isolating device provided in an embodiment of the present invention；
Figure 10 is the structural schematic diagram two of elastic sealing gasket in isolating device provided in an embodiment of the present invention；
Figure 11 is the structural schematic diagram of upper cover in isolating device provided in an embodiment of the present invention.
In figure:
11- ontology；12- upper cover；13- partition；131- limits inserted block；14- limit hole；15- fixture block；16- reagent
Hole；20- elastic sealing gasket；The first designated port of 21-；The second designated port of 22-；23- male part；24- pad；30- support plate；
31- long hole；40- limits socket；50- reagent holding mechanism.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented
A part of example can be arranged and be designed by a variety of different configurations.
Therefore, the detailed description of the embodiment of the present invention provided in the accompanying drawings is not intended to limit below claimed
The scope of the present invention, but be merely representative of selected embodiment of the invention.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
It should also be noted that similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined in a attached drawing, does not then need that it is further defined and explained in subsequent attached drawing.
In the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, or be somebody's turn to do
Invention product using when the orientation or positional relationship usually put, be merely for convenience of description of the present invention and simplification of the description, without
It is that the isolating device of indication or suggestion meaning or element must have a particular orientation, be constructed and operated in a specific orientation, because
This is not considered as limiting the invention.In addition, term " first ", " second ", " third " etc. are only used for distinguishing description, without
It can be interpreted as indication or suggestion relative importance.
In addition, the terms such as term "horizontal", "vertical", " pendency " are not offered as requiring component abswolute level or pendency, and
It is that can be slightly tilted.It is not to indicate the structure if "horizontal" only refers to that its direction is more horizontal with respect to for "vertical"
It has to fully horizontally, but can be slightly tilted.
In the description of the present invention, it is also necessary to which explanation is unless specifically defined or limited otherwise, term " setting ",
" installation ", " connected ", " connection " shall be understood in a broad sense, for example, it may be fixedly connected, may be a detachable connection or one
Connect to body；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, it can also be indirect by intermediary
It is connected, can be the connection inside two elements.For the ordinary skill in the art, on being understood with concrete condition
State the concrete meaning of term in the present invention.
With reference to the accompanying drawing, it elaborates to some embodiments of the present invention.In the absence of conflict, following
Feature in embodiment and embodiment can mutually conjunction.
First embodiment
It please refers to shown in Fig. 1-Fig. 3, Figure 11, the present embodiment provides a kind of isolating device, including shell, includes in shell
Two or more mutually isolated receiving areas, receiving area are accommodated for accommodating at least one reagent in reagent holding mechanism 50
Unit.
Shell corresponds to each receiving area and is respectively arranged with limit hole 14, and limit hole 14 is connected to corresponding receiving area, limit hole
14 length direction is identical as the length direction of a reagent housing unit, exists between limit hole 14 and reagent housing unit and moves
Dynamic space, mobile space are used for the active section movement for nucleic acid extraction mechanism.
It specifically, include reagent holding mechanism 50 and nucleic acid extraction mechanism, specifically, reagent in instrument for extracting nucleic acid
Holding mechanism 50 includes multiple reagent housing units, and each reagent receiving member includes multiple holding tanks, and each holding tank is along examination
The length direction of agent housing unit arranges, and during the work time, the nucleic acid extraction part in nucleic acid extraction mechanism is sequentially inserted into a certain
A holding tank, when in nucleic acid extraction mechanism including multiple nucleic acid extraction parts, each nucleic acid extraction part can respectively correspond an examination
Agent housing unit, so set, multiple nucleic acid extraction work can be carried out in the same time.
In isolating device, shell is divided into multiple mutually isolated receiving areas by partition 13, in reagent holding mechanism 50
Multiple reagent housing units can be put in different receiving areas respectively, thus by multiple reagent housing units be divided at least two parts into
Row is isolated, and the reagent in different receiving areas will not mutually generate cross-infection.
The quantity of receiving area can be two or more in shell, and each receiving area at least accommodates a reagent and accommodates
Unit.For example, when needing to be at best able in shell to accommodate ten reagent housing units, following several setting sides can be used
Formula, shell is interior to have ten receiving areas, and each receiving area is respectively used to accommodate a reagent housing unit.Alternatively, having in shell
Five receiving areas, each receiving area are respectively used to accommodate two reagent housing units.Alternatively, there are two receiving areas for tool in shell, respectively
Receiving area is respectively used to accommodate five reagent housing units.Certainly, in addition to above-mentioned set-up mode, there are also other set-up modes,
This is repeated no more.
Shell can be overall structure in the present embodiment a kind of, also may include multiple disassembling boxes.
When shell include multiple disassembling boxes when, each disassembling box can dismantling connection, each disassembling box have a receiving area.
So set, in use, corresponding number can be selected according to the number of the reagent housing unit in reagent holding mechanism 50
Disassembling box carry out dress, to form corresponding shell.For example, reagent holding mechanism 50 includes three reagent housing units, then
Three disassembling boxes are sequentially connected, and three reagent housing units are correspondingly placed into three receiving areas.For another example reagent accommodates
Mechanism 50 includes four reagent housing units, then is sequentially connected four disassembling boxes, and four reagent housing unit correspondences are put
Enter in four receiving areas.
Alternatively, in another usage scenario, although reagent holding mechanism 50 includes four reagent housing units, only
Have in two of them reagent housing unit and accommodate reagent, used for extracting, two disassembling boxes may be used, will place respectively
There is two reagent housing units correspondence of reagent to be placed in two receiving areas.
When shell is overall structure, shell includes the ontology 11 being detachably connected and upper cover 12, and upper cover 12 is covered on this
Body 11 is provided with partition 13 in ontology 11, and ontology 11 is divided into multiple receiving areas by partition 13, and limit hole 14 is set to upper cover
12.So set, convenient for placing reagent housing unit into shell.When picking and placing test tube housing unit, by upper cover 12 from ontology
It removes on 11, after pick-and-place movement, upper cover 12 is again mounted on ontology 11.
There are many connection types between upper cover 12 and ontology 11, such as is connected by connectors such as bolt, pin shaft, buckles
It connects, alternatively, as shown in figure 4, specifically, be provided with card slot in upper cover 12 to be connected together between upper cover 12 and ontology 11,
The region that card slot is corresponded on ontology 11 is provided with fixture block 15, and after upper cover 12 is fastened on ontology 11, fixture block 15 protrudes into card slot, from
And upper cover 12 is engaged with ontology 11.Further, the edge of upper cover 12 is provided with the flange extended to 11 direction of ontology, card slot
It is set to the inside of flange, fixture block 15 is set on the outside of the side plate of ontology 11.When upper cover 12 is fastened on ontology 11, flange packet
It is overlying on the outside of the upper area of ontology 11, fixture block 15 protrudes into card slot.It certainly, can also be by card slot in another set-up mode
It is set to 11 outside of ontology, fixture block 15 is set on the inside of the flange of upper cover 12.
As shown in Fig. 1 and Fig. 9, elastic sealing gasket 20 is provided between ontology 11 and upper cover 12, elastic sealing gasket 20 is corresponding
The region of limit hole 14 is provided with the first designated port 21, the length direction phase of the length direction and limit hole 14 of the first designated port 21
Together.So set, making at the first designated port 21 in closed state on elastic sealing element when nucleic acid extraction mechanism does not extend into shell
Elastic sealing element is obtained completely to seal receiving area.When nucleic acid extraction mechanism protrudes into shell, nucleic acid extraction mechanism is by the first designated port
It is protruded at 21, the region that 21 side of the first designated port is located on elastic sealing element deforms under the extruding of nucleic acid extraction mechanism, thus for
Nucleic acid extraction mechanism protrudes into.After nucleic acid extraction mechanism protrudes into shell, it may make at limit hole 14 occur using elastic sealing element
Opening can either be protruded into for various sizes of nucleic acid extraction mechanism, and can guarantee that opening size is relatively smaller.
Preferably, as shown in figure 9, being provided with multiple second designated ports 22 on elastic sealing gasket 20, the second designated port 22 and first
Designated port 21 intersects, and each second designated port 22 is arranged along the length direction interval of the first designated port 21.So set, the second designated port 22 and
The intersection of one designated port 21 is more likely to form bigger opening, and for nucleic acid extraction, mechanism is protruded into.
In Fig. 9, the first designated port 21 is vertical with the second designated port 22, and the first designated port 21 is located at 22 intersection of the second designated port
The central region of second designated port 22.
Elastic sealing gasket 20 can be made of rubber or silica gel material, and the first designated port 21 and the second designated port 22 can be by knives
Tool is formed on elastic sealing gasket 20 along straight cuts.
As shown in Figure 10, in a specific embodiment, elastic sealing gasket 20 includes pad 24, corresponding limit on pad 24
The region in position hole 14 is provided with male part 23, and male part 23 protrudes into limit hole 14, and the first designated port 21 is set to male part 23.So
Setting works as isolating device after the assembly is completed since elastic sealing gasket 20 is located at the lower section of upper cover 12, elastic sealing gasket 20
Male part 23 can protrude into the limit hole 14 of upper cover 12.The setting of male part 23 is convenient for close by the limit hole 14 of upper cover 12 and elasticity
The first designated port 21 on packing 20 is aligned.
As shown in Figure 3 and Figure 4, in a preferred embodiment, branch is provided between ontology 11 and elastic sealing gasket 20
Fagging 30, the region opposite with limit hole 14 is provided with long hole 31 in support plate 30.Specifically, long hole 31 and limit hole 14 be one by one
It is correspondingly arranged.So set, support plate 30 is located at the lower section of elastic sealing gasket 20, support is played for elastic sealing gasket 20 and is made
With avoiding elastic sealing gasket 20 from deforming on the inside of receiving area.Long hole 31 in support plate 30 is used for for across elastic sealing gasket 20
Nucleic acid extraction mechanism protrude into receiving area.
As shown in Figure 4-Figure 7, it is provided with limit inserted block 131 on partition 13, is provided on elastic sealing gasket 20 and shell
Limit socket 40, limit inserted block 131 sequentially passes through the limit socket 40 that shell is protruded into after the limit socket 40 of elastic sealing gasket 20.
Further, limit socket 40 is also equipped in support plate 30, limit inserted block 131 sequentially passes through the limit in support plate 30 and inserts
Mouthfuls 40, the limit socket 40 of shell is protruded into after the limit socket 40 of elastic sealing gasket 20.So set, convenient for by ontology 11, on
Lid 12, support plate 30 and elastic sealing element are limited, and prevent above structure from occurring play between each other.In addition it is also possible to logical
It crosses limit inserted block 131 and limit socket 40 plays the role of contraposition, so that limit hole 14, long hole 31, the first designated port 21 and receiving area
Contraposition is accurate.
In a kind of specific embodiment of the present embodiment, fixed frame is provided in shell, reagent housing unit is placed in
On fixed frame, and it is relatively fixed by fixed frame and shell.
Alternatively, as shown in Figure 5 and Figure 8, in a preferred embodiment, the side opposite with limit hole 14 is set on shell
Reagent wells 16 are equipped with, reagent wells 16 are used to reach hull outside for the partial region of reagent housing unit, but reagent accommodates
The opening of holding tank is respectively positioned in accommodating chamber in unit.So set, further reducing the size of shell, system is reduced
This is caused, isolating device weight is reduced.
Preferably, the reagent wells 16 of shell are configured as, and are stretched out for most of region of reagent housing unit, only holding tank
Opening outer region be erected on case inside, and contacted with the inner wall of the shell side.
Second embodiment
The present embodiment provides a kind of instrument for extracting nucleic acid comprising the isolating device that above-mentioned first embodiment provides, including it is outer
Shell, the inner cavity of shell are provided with nucleic acid extraction mechanism, reagent holding mechanism and isolating device, and reagent holding mechanism 50 includes multiple
Reagent housing unit, reagent housing unit are provided with multiple holding tanks, each holding tank at least one reagent housing unit
Opening is respectively positioned on the inside of isolating device, and the limit hole that nucleic acid extraction mechanism can pass through isolating device protrudes into holding tank.
That is, only one of reagent housing unit can be placed on the inside of isolating device, so that changing an examination
Agent housing unit is isolated with other reagent housing units.Multiple reagent housing units can also be respectively put into isolating device not
Same receiving area is isolated into two parts or multi-section point.
Preferably, each reagent housing unit is placed in isolating device, and in each receiving area of isolating device, at most puts
It is equipped with a reagent housing unit, so that each reagent housing unit is isolated between each other.
In a specific embodiment, reagent housing unit includes multiple test tubes, is provided with holding tank in test tube.It is same
The opening outer of multiple test tubes in reagent housing unit is connected, and forms townhouse pipe.
Alternatively, reagent housing unit can be the block structure of an entirety, it is arranged in the wherein side of the bulk mechanism
There is row's holding tank.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of isolating device, which is characterized in that including shell, in the shell include that two or more are mutually isolated
Receiving area, the receiving area is for accommodating at least one reagent housing unit；
The shell corresponds to each receiving area and is respectively arranged with the limit hole being connected to the receiving area, the length of the limit hole
It is identical as the length direction of the reagent housing unit to spend direction, exists between the limit hole and the reagent housing unit and moves
Dynamic space, the mobile space are used for the active section movement for nucleic acid extraction mechanism.
2. isolating device according to claim 1, which is characterized in that the shell includes ontology and upper cover, the upper cover
It is covered on the ontology, partition is provided in the ontology, the ontology is divided into multiple receiving areas, institute by the partition
It states limit hole and is set to the upper cover, the ontology is detachably connected with the upper cover.
3. isolating device according to claim 2, which is characterized in that be arranged between the ontology and the upper cover flexible
Gasket, the region that the elastic sealing gasket corresponds to the limit hole are provided with the first designated port, the length of first designated port
Direction is identical as the length direction of the limit hole.
4. isolating device according to claim 3, which is characterized in that be provided with multiple second strokes on the elastic sealing gasket
Mouthful, second designated port intersects with first designated port, and each second designated port is along the length direction interval of first designated port
Setting.
5. isolating device according to claim 3, which is characterized in that the elastic sealing gasket includes pad, the pad
The region of the upper correspondence limit hole is provided with male part, and the male part protrudes into the limit hole, the first designated port setting
In the male part.
6. isolating device according to claim 3, which is characterized in that be arranged between the ontology and the elastic sealing gasket
There is a support plate, the region opposite with the limit hole is provided with long hole in the support plate.
7. isolating device according to claim 3, which is characterized in that be provided with limit inserted block, the bullet on the partition
Property gasket and the shell on be provided with limit socket, the limit inserted block sequentially passes through the limit of the elastic sealing gasket
The limit socket of the shell is protruded into after socket.
8. isolating device according to claim 1, which is characterized in that the side opposite with the limit hole on the shell
Reagent wells are provided with, the reagent wells are used to reach the hull outside for the partial region of the reagent housing unit.
9. isolating device according to claim 1, which is characterized in that the shell is connected by multiple disassembling boxes and formed, respectively
The disassembling box can be detachably connected, and each disassembling box has a receiving area.
10. a kind of instrument for extracting nucleic acid, which is characterized in that it includes shell, the inner cavity of the shell be provided with nucleic acid extraction mechanism,
Reagent holding mechanism and the isolating device as described in claim 1-9 is any, the reagent holding mechanism hold comprising multiple reagents
Receive unit, the reagent housing unit is provided with multiple holding tanks, and each holding tank opens at least one reagent housing unit
Mouth is respectively positioned on the inside of the isolating device, and the limit hole that the nucleic acid extraction mechanism can pass through the isolating device protrudes into described
Holding tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910723629.1A CN110257225A (en) | 2019-08-06 | 2019-08-06 | Isolating device and instrument for extracting nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910723629.1A CN110257225A (en) | 2019-08-06 | 2019-08-06 | Isolating device and instrument for extracting nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110257225A true CN110257225A (en) | 2019-09-20 |
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ID=67912847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910723629.1A Pending CN110257225A (en) | 2019-08-06 | 2019-08-06 | Isolating device and instrument for extracting nucleic acid |
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| CN (1) | CN110257225A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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