CN110227564B - Detection system based on micro-fluidic technology - Google Patents
Detection system based on micro-fluidic technology Download PDFInfo
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- CN110227564B CN110227564B CN201910521739.XA CN201910521739A CN110227564B CN 110227564 B CN110227564 B CN 110227564B CN 201910521739 A CN201910521739 A CN 201910521739A CN 110227564 B CN110227564 B CN 110227564B
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
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Abstract
The invention relates to the technical field of microfluidics, in particular to a detection system based on the microfluidics technology. The device comprises a high-pressure air source mechanism, a sample introduction mechanism, a feeding mechanism and a micro-fluidic chip, wherein the feeding mechanism is used for adding a sample into the sample introduction mechanism, the high-pressure air source mechanism is used for providing a high-pressure air source for the sample introduction mechanism so as to press the sample in the sample introduction mechanism into the micro-fluidic chip, and the micro-fluidic chip is used for analyzing and processing the sample. The invention has simple structure and convenient operation.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a detection system based on the microfluidics technology.
Background
Microfluidics (Microfluidics), which refers to the science and technology involved in systems using microchannels (tens to hundreds of microns in size) to process or manipulate tiny fluids (nanoliters to attoliters in volume), is an emerging interdiscipline of chemistry, fluid physics, microelectronics, new materials, biology and biomedical engineering. Because of their miniaturization, integration, etc., microfluidic devices are commonly referred to as microfluidic chips, also known as Lab-on-a-chips (Lab-on-a-chips) and micro-total analytical systems (micro-total analytical systems). The early concept of microfluidics can be traced back to gas chromatographs fabricated on silicon wafers by photolithography in the 70 s of the 19 th century, and then developed into microfluidic capillary electrophoresis instruments, microreactors and the like. One of the important features of microfluidics is the unique fluid properties in microscale environments, such as laminar flow and droplets. With these unique fluidic phenomena, microfluidics can achieve a range of microfabrication and micromanipulation that are difficult to accomplish with conventional methods. At present, microfluidics is considered to have great development potential and wide application prospect in biomedical research
The micro-fluidic system performs preparation, reaction, separation, detection and other operations on the fluid in the micro-nano scale space. The micro-fluidic system mainly comprises a micro-fluidic chip, a liquid path sample introduction system and a detection system. The liquid path sample introduction system is used for injecting a sample into the microscopic microfluidic chip for analysis. At present, the existing liquid path sample injection system is complex in structure and operation.
Disclosure of Invention
It is an object of the present invention to provide a detection system based on microfluidic technology which overcomes some or some of the disadvantages of the prior art.
The detection system based on the microfluidic technology comprises a high-pressure air source mechanism, a sample feeding mechanism, a feeding mechanism and a microfluidic chip, wherein the feeding mechanism is used for adding a sample into the sample feeding mechanism;
the sample feeding mechanism comprises a cylindrical sample feeding mechanism main body, and a plurality of independent sample cavities are arranged in the sample feeding mechanism main body; the sample cavity extends inwards from the lower end face of the sample feeding mechanism main body along the axial direction, and the lower end of the sample feeding mechanism main body is provided with a sealing bottom plate for sealing the sample cavity; the middle part of the upper end surface of the sampling mechanism main body extends inwards to form an air inlet cavity, the upper end of the sampling mechanism main body is provided with a sealing cover plate for sealing the air inlet cavity, and the sealing cover plate is provided with an air inlet for connecting with a high-pressure air source mechanism;
air flow channels are arranged between the air inlet cavity and any sample cavity along the radial direction, air valve mounting holes for mounting an air valve mechanism are axially arranged on the upper end surface of the sampling mechanism main body corresponding to any air flow channel, and the air valve mechanism is used for adjusting the effective air inlet section area of the air flow channel; the upper end surface of the sample injection mechanism main body is axially provided with a sample injection port communicated with the sample cavity, and the sample injection port is used for being connected with the feeding mechanism; and a sample outlet communicated with the sample cavity is radially arranged on the side surface of the sample injection mechanism body and is used for being connected with the microfluidic chip.
In the detection system, the high-pressure air source provided by the high-pressure air source mechanism can better press the samples in the sample cavity into the microfluidic chip, and because a plurality of sample cavities can be arranged, the simultaneous sample injection of different samples can be better realized, so that the structure is simple, and the operation is simple and convenient. In addition, due to the arrangement of the air valve mechanism, the effective air inlet section area of the air flow channel can be better adjusted, so that the air inlet flow in each sample cavity can be better adjusted, the sample injection pressure of each sample cavity can be better adjusted, and the simultaneous sample injection among samples with different sample injection amounts can be better met.
Preferably, the cross section of the air flow channel is square, a valve core movable hole with a rectangular cross section is formed at the lower part of the air valve mounting hole, and a valve seat mounting hole with a circular cross section is formed at the upper part of the air valve mounting hole; the length direction of the cross section of the valve core movable hole is vertical to the extension direction of the air flow channel, and the valve core movable hole and the corresponding inner wall of the air flow channel are on the same vertical plane; the valve core is movably arranged in the valve core movable hole up and down, the valve core is hermetically matched with the valve core movable hole and the air flow channel, and the heights of the valve core, the valve core movable hole and the air flow channel in the vertical direction are the same; a valve rod is vertically arranged above the valve core, a valve rod fixing ring is arranged at the bottom of the valve seat mounting hole, a valve rod guide hole for sliding fit of the valve rod is formed in the middle of the valve rod fixing ring, and the valve rod is in sealing fit with the valve rod guide hole; the valve seat mounting hole is provided with a bearing mounting ring above the valve rod fixing ring, a bearing is arranged at the inner hole of the bearing mounting ring, an adjusting ring is arranged at the inner hole of the bearing, and the inner hole of the adjusting ring is in threaded fit with the valve rod.
In the detection system, the lifting distance of the valve core can be preferably adjusted by adjusting the adjusting ring through the matching of the bearing mounting ring, the bearing and the adjusting ring; the cross section of the air flow channel is square, so that the lifting distance of the valve core can be in direct proportion to the conduction area of the air flow channel, and the conduction area of the air flow channel can be better adjusted; and because the valve core, the valve core movable hole and the air flow channel have the same height in the vertical direction, the air flow channel is completely communicated when the valve core is at the upper limit position and completely closed when the valve core is at the lower limit position, and the communication area of the air flow channel is convenient to adjust.
Preferably, the thread lead of the adjusting ring is equal to the height of the spool in the vertical direction. Thereby make the adjustable ring rotation a week, can realize that the case seals the complete of air runner completely and switches on to the air runner to can realize the control to case distance from top to bottom better through the control to adjustable ring rotation angle, again because the area of switching on of air runner is directly proportional with the upper and lower distance of case, and then can realize the more accurate regulation to air runner area of switching on better, and then can adjust according to the area of switching on of the appearance ratio air runner of different samples better.
Preferably, a plurality of scale marks are uniformly arranged on the upper surface of the adjusting ring along the circumferential direction, a positioning bayonet is arranged on the side surface of the adjusting ring corresponding to any scale mark, a positioning mark block matched with the positioning bayonet is arranged on the main body of the sample feeding mechanism, and the positioning mark block is detachably inserted into the main body of the sample feeding mechanism. Through the setting of many scale marks, can realize the regulation to regulation ring rotation angle comparatively accurately. In addition, through the design of the positioning bayonet and the positioning mark block, the marking of the rotation angle of the adjusting ring can be better realized, and the fixing of the rotation position of the adjusting ring can also be effectively realized. The positioning mark block is a cylinder with a cross section being in a regular triangle shape, and the sampling mechanism body is provided with a regular triangle jack for inserting the positioning mark block, so that after the positioning mark block is pulled out, the adjusting ring can be adjusted better, and the adjusting ring can be fixed better by inserting the positioning mark block.
Preferably, the lower end of the adjusting ring is provided with an adjusting ring mounting part matched with the bearing, the upper end of the adjusting ring is provided with an adjusting ring dial part, and the positioning bayonet is arranged at the adjusting ring dial part. So that the mounting and the striking of the adjusting ring can be advantageously facilitated.
Preferably, the valve core and the valve rod are of an integral structure. Thereby facilitating the manufacture of the valve core and the valve rod.
Drawings
FIG. 1 is a schematic system block diagram of a detection system according to embodiment 1;
FIG. 2 is a schematic view of a sample injection mechanism in example 1;
fig. 3 is a schematic view of a valve train in embodiment 1;
fig. 4 is a schematic top view of the valve train in embodiment 1;
FIG. 5 is a schematic view of an adjusting ring in embodiment 1;
FIG. 6 is a schematic view of the cartridge and stem of embodiment 1;
FIG. 7 is a schematic view of a sample injection mechanism main body in embodiment 1;
FIG. 8 is a schematic half-sectional view of a sample injection mechanism main body according to embodiment 1;
FIG. 9 is a schematic view of a sealing bottom plate in example 1;
FIG. 10 is a schematic view of a feed valve in example 1;
FIG. 11 is a schematic view of a feeding mechanism in example 1;
fig. 12 is a schematic view of a feed spool in example 1.
Detailed Description
For a further understanding of the invention, reference should be made to the following detailed description taken in conjunction with the accompanying drawings and examples. It is to be understood that the examples are illustrative of the invention and not limiting.
Example 1
Referring to fig. 1, the present embodiment provides a detection system based on a microfluidic technology, which includes a high-pressure air source mechanism, a sample injection mechanism 200, a feeding mechanism 1100, and a microfluidic chip, where the feeding mechanism 1100 is configured to add a sample into the sample injection mechanism 200, the high-pressure air source mechanism is configured to provide a high-pressure air source to the sample injection mechanism 200 to press the sample in the sample injection mechanism 200 into the microfluidic chip, and the microfluidic chip is configured to analyze the sample.
As shown in fig. 2, the sample injection mechanism 200 includes a cylindrical sample injection mechanism main body 210, and a plurality of independent sample cavities 211 are provided in the sample injection mechanism main body 210; the sample cavity 211 extends inwards from the lower end face of the sample feeding mechanism main body 210 along the axial direction, and the lower end of the sample feeding mechanism main body 210 is provided with a sealing bottom plate 220 for sealing the sample cavity 211; the middle part of the upper end face of the sampling mechanism main body 210 extends inwards to form an air inlet cavity 212, the upper end of the sampling mechanism main body 210 is provided with a sealing cover plate 230 for sealing the air inlet cavity 212, and the sealing cover plate 230 is provided with an air inlet 231 for connecting with a high-pressure air source mechanism;
an air flow channel 213 is arranged between the air inlet cavity 212 and any sample cavity 211 along the radial direction, an air valve mounting hole 214 for mounting an air valve mechanism 300 is arranged at the upper end surface of the sample injection mechanism main body 210 corresponding to any air flow channel 213 along the axial direction, and the air valve mechanism 300 is used for adjusting the effective air inlet section area of the air flow channel 213; the upper end surface of the sample injection mechanism main body 210 is axially provided with a sample injection port 215 communicated with the sample cavity 211, and the sample injection port 215 is used for being connected with the feeding mechanism 1100; the side surface of the sample injection mechanism main body 210 is provided with a sample outlet 216 communicated with the sample cavity 211 along the radial direction, and the sample outlet 216 is used for connecting with a microfluidic chip.
In the detection system of the embodiment, the high-pressure air source provided by the high-pressure air source mechanism can press the sample in the sample cavity 211 into the microfluidic chip better, and the sample cavity 211 can be provided with a plurality of sample cavities, so that simultaneous sample introduction of different samples can be realized better, and the detection system is simple in structure and easy and convenient to operate. In addition, due to the arrangement of the air valve mechanism 300, the effective air inlet cross-sectional area of the air flow channel 213 can be preferably adjusted, so that the air inlet flow in each sample cavity 211 can be preferably adjusted, and the sample injection pressure of each sample cavity 211 can be preferably adjusted, thereby better satisfying the simultaneous sample injection among samples with different sample injection amounts.
Referring to fig. 3, the air flow passage 213 has a square cross section, a valve core moving hole 311 having a rectangular cross section is formed at the lower portion of the air valve mounting hole 214, and a valve seat mounting hole 312 having a circular cross section is formed at the upper portion of the air valve mounting hole 214; the length direction of the cross section of the valve core movable hole 311 is vertical to the extension direction of the air flow channel 213, and the valve core movable hole 311 and the corresponding inner wall of the air flow channel 213 are on the same vertical plane; the valve core 320 is movably arranged in the valve core movable hole 311 up and down, the valve core 320 is hermetically matched with the valve core movable hole 311 and the air flow passage 213, and the heights of the valve core 320, the valve core movable hole 311 and the air flow passage 213 in the vertical direction are the same; a valve rod 330 is vertically arranged above the valve core 320, a valve rod fixing ring 340 is arranged at the bottom of the valve seat mounting hole 312, a valve rod guide hole 341 for sliding fit of the valve rod 330 is arranged in the middle of the valve rod fixing ring 340, and the valve rod 330 is in sealing fit with the valve rod guide hole 341; the valve seat mounting hole 312 is provided with a bearing mounting ring 350 above the valve rod fixing ring 340, a bearing 360 is arranged in the inner hole of the bearing mounting ring 350, an adjusting ring 370 is arranged in the inner hole of the bearing 360, and the inner hole of the adjusting ring 370 is in threaded fit with the valve rod 330.
In the detection system of the present embodiment, the lifting distance of the valve element 320 can be preferably adjusted by adjusting the adjusting ring 370 through the cooperation of the bearing mounting ring 350, the bearing 360 and the adjusting ring 370; the cross section of the air flow channel 213 is square, so that the lifting distance of the valve core 320 can be in direct proportion to the conduction area of the air flow channel 213, and the conduction area of the air flow channel 213 can be better adjusted; and because the valve core 320, the valve core movable hole 311 and the air flow channel 213 have the same height in the vertical direction, the air flow channel 213 is completely communicated when the valve core 320 is at the upper limit position, and the air flow channel 213 is completely cut off when the valve core 320 is at the lower limit position, thereby being convenient for adjusting the communication area of the air flow channel 213.
In this embodiment, the thread lead of the adjustment ring 370 is equal to the height of the valve spool 320 in the vertical direction. Therefore, the adjusting ring 370 rotates for a circle, so that the valve core 320 can completely close the air flow channel 213 to completely communicate with the air flow channel 213, and the control of the vertical distance of the valve core 320 can be better realized by controlling the rotation angle of the adjusting ring 370, and the communication area of the air flow channel 213 is in direct proportion to the vertical distance of the valve core 320, so that the precise adjustment of the communication area of the air flow channel 213 can be better realized, and the communication area of the air flow channel 213 can be better adjusted according to the sampling ratio of different samples.
Referring to fig. 4, a plurality of graduation lines 410 are uniformly arranged on the upper surface of the adjusting ring 370 along the circumferential direction, a positioning bayonet 430 is arranged on the side surface of the adjusting ring 370 corresponding to any graduation line 410, a positioning mark block 420 for matching with the positioning bayonet 430 is arranged on the main body 210 of the sample injection mechanism, and the positioning mark block 420 is detachably inserted into the main body 210 of the sample injection mechanism. Through the setting of many scale marks 410, can realize the regulation to regulation ring 370 rotation angle comparatively accurately. In addition, through the design of the positioning bayonet 430 and the positioning mark block 420, not only the marking of the rotation angle of the adjustment ring 370 can be preferably realized, but also the fixing of the rotation position of the adjustment ring 370 can be effectively realized. The positioning mark block 420 is a cylinder with a regular triangle cross section, and the sampling mechanism body 210 is provided with a regular triangle jack for inserting the positioning mark block 420, so that the adjusting ring 370 can be adjusted better after the positioning mark block 420 is pulled out, and the adjusting ring 370 can be fixed better by inserting the positioning mark block 420.
Referring to fig. 5, an adjusting ring mounting portion 510 is formed at a lower end of the adjusting ring 370 to be engaged with the bearing 360, an adjusting ring dial portion 520 is formed at an upper end of the adjusting ring 370, and the positioning bayonet 430 is disposed at the adjusting ring dial portion 520. Thereby preferably facilitating installation and dialing of adjustment ring 370.
As shown in fig. 6, the valve core 320 and the valve stem 330 are of an integral structure. Thereby facilitating the manufacture of the valve cartridge 320 and the valve stem 330.
Referring to fig. 7 and 8, the outer side of the upper end of the sample chamber 211 extends upward to form a sample chamber buffer 211a, and the air channel 213 and the sample inlet 215 are both directly communicated with the sample chamber buffer 211a, so that sample addition and air flow entry can be preferably facilitated. In addition, a sealing cover plate installation groove 810 for installing the sealing cover plate 230 is formed at the opening of the air inlet cavity 212, thereby facilitating the arrangement of the sealing cover plate 230; the lower end surface of the sample injection mechanism main body 210 forms a sealing bottom plate installation groove 820 for installing the sealing bottom plate 220, thereby facilitating the installation of the sealing bottom plate 220. With such a structure of the sample injection mechanism main body 210, the mold opening of the sample injection mechanism main body 210 can be preferably facilitated, and the manufacturing is further facilitated.
As shown in fig. 9, the sealing bottom plate 220 includes a bottom plate 910, and the diameter of the bottom plate 910 is the same as that of the sample injection mechanism main body 210; a sealing bottom plate mounting table 920 used for being matched with the sealing bottom plate mounting groove 820 is arranged above the bottom plate 910, and a sample cavity plug part 930 used for being in sealing fit with an opening at the lower end of the sample cavity 211 is arranged at the position, corresponding to any sample cavity 211, of the sealing bottom plate mounting table 920, so that the tightness of the sample cavity 211 can be better ensured; the upper surface of any sample cavity plugging part 930 is provided with 2 sample guiding grooves 940 which are orthogonally arranged, one of the 2 sample guiding grooves 940 is arranged along the radial direction of the sample feeding mechanism main body 210, and the position of the sample outlet 216 corresponds to the sample guiding groove 940 which is arranged along the radial direction, so that the sample can be conveniently flowed out.
The high-pressure gas source mechanism, the sample injection mechanism 200 and the feeding mechanism 1100 in this embodiment can independently constitute a sample injection system, which can be preferably applied to various microfluidic systems.
Referring to fig. 10, a feed valve 1000 is disposed at the sample inlet 215, the feed valve 1000 includes a feed valve seat 1010, and a feed valve core mounting hole 1011 is axially disposed in the feed valve seat 1010 in a penetrating manner; a feeding valve core 1020 is slidably arranged in the feeding valve core mounting hole 1011, and the feeding valve core 1020 is in sealing fit with the feeding valve core mounting hole 1011; a feeding cavity 1021 with an upward opening is axially arranged in the feeding valve core 1020, and a feeding channel 1022 communicated with the feeding cavity 1021 is radially arranged on the side wall of the lower part of the feeding valve core 1020; the middle part of the feeding valve core 1020 extends towards the periphery to form a feeding valve core blocking ring part 1023, a first spring cavity 1012 which is in clearance fit with the valve core blocking ring part 1023 is arranged at the upper end of the valve core mounting hole 1011, and a first spring 1013 sleeved at the feeding valve core 1020 is arranged between the bottom wall of the first spring cavity 1012 and the valve core blocking ring part 1023; a feeding valve core limiting ring 1014 is arranged at the opening of the first spring cavity 1012, and the feeding valve core limiting ring 1014 is used for limiting the ascending position of the feeding valve core 1020; the feed channel 1022 is located within the feed valve spool mounting hole 1011 when the feed valve spool 1020 is in the upper limit position, and the feed channel 1022 is located below the feed valve spool mounting hole 1011 when the feed valve spool 1020 is in the lower limit position.
In the sample injection system of this embodiment, when sample injection is required, the feeding mechanism 1100 can extrude the feeding valve core 1020, so that the feeding channel 1022 conducts the feeding cavity 1021 and the sample cavity 211, and thus a sample can be better added into the sample cavity 211; after the sample injection is completed, under the action of the first spring 1013, the feeding valve core 1020 can always keep the upper limit position, so that the feeding cavity 1021 and the sample cavity 211 can be better isolated, and the sample in the sample cavity 211 can be conveniently extruded.
As shown in fig. 11, the feeding mechanism 1100 includes a feeding mechanism main body 1110, a sample storage chamber 1111 is disposed in the feeding mechanism main body 1110, and a sample adding port 1112 communicated with the sample storage chamber 1111 is disposed at an upper end of the feeding mechanism main body 1110; a discharge valve mounting groove 1113 is formed in the lower end face of the feeding mechanism main body 1110, and a discharge valve core mounting hole 1114 communicated with the sample storage cavity 1111 is formed in the middle of the bottom wall of the discharge valve mounting groove 1113; a discharge valve core 1120 is arranged in the discharge valve core mounting hole 1114, a discharge cavity 1121 with an opening facing the sample storage cavity 1111 is axially arranged in the discharge valve core 1120, and a discharge channel 1122 communicated with the discharge cavity 1121 is radially arranged on the side wall of the lower part of the discharge valve core 1120; a discharge channel sealing ring 1130 is slidably sleeved on the discharge valve core 1120, and the discharge channel sealing ring 1130 is in sealing fit with the discharge valve core 1120; a second spring 1140 is arranged between the discharge channel sealing ring 1130 and the bottom wall of the discharge valve mounting groove 1113, and a sealing ring limiting ring 1150 for limiting the descending position of the discharge channel sealing ring 1130 is arranged at the position, below the discharge channel sealing ring 1130, of the discharge valve mounting groove 1113; when the discharge channel sealing ring 1130 is located at the lower limit position, the discharge channel 1122 is sealed by the discharge channel sealing ring 1130, and when the discharge channel sealing ring 1130 is located at the upper limit position, the discharge channel 1122 is located below the discharge channel sealing ring 1130; the upper end of the feeding valve core limiting ring 1014 forms a limiting ring extrusion part 1014a used for matching with the discharging channel sealing ring 1130, and the inner hole diameter of the sealing ring limiting ring 1150 is larger than the outer diameter of the limiting ring extrusion part 1014 a.
In the sample sampling system of this embodiment, when a sample needs to be added, the discharge valve core 1120 can be inserted into the feeding cavity 1021 of the feeding valve core 1020, and then the feeding mechanism 1100 is pressed down, so that the feeding valve core 1020 can move down until the feeding cavity 1021 and the sample cavity 211 are conducted, and along with the continuous movement of the feeding mechanism 1100, the limiting ring pressing portion 1014a can press the sealing ring 1130 until the discharge channel 1122 is exposed out of the sealing ring 1130, and at this time, the addition of the sample can be preferably realized.
In the sample injection system of this embodiment, the lower end of the discharging valve core 1120 is retracted into the discharging valve installation groove 1113. Thereby enabling the feeding mechanism 1100 to be preferably placed upright.
In the sample injection system of this embodiment, the feeding valve core limiting ring 1014 is in threaded fit with the first spring cavity 1012, and the middle portion of the feeding valve core limiting ring 1014 extends towards the outer periphery to form a feeding valve core limiting ring flange 1014 b. Thereby preferably facilitating installation of the feed spool stop collar 1014.
In the sample injection system of this embodiment, the middle portion of the discharging valve core 1120 extends to the outer periphery to form a discharging valve core edge blocking portion 1123 for matching with the bottom wall of the discharging valve installation groove 1113, and the second spring 1140 is located between the discharging valve core edge blocking portion 1123 and the discharging channel sealing ring 1130. Installation of the discharge spool 1120 can be advantageously facilitated.
In the sample injection system of this embodiment, the feeding valve core 1020 and the discharging valve core 1120 are both an integrated structure. Thereby facilitating production and manufacture.
Referring to fig. 12, an air flow channel 1210 is disposed at the position of the feed valve core 1020, the upper and lower openings of the air flow channel 1210 are disposed at the side surface of the feed valve core 1020, the axis of the lower opening of the air flow channel 1210 and the axis of the feed channel 1022 are on the same horizontal plane, and the upper opening of the air flow channel 1210 is always located above the feed valve core retainer ring 1014. This allows the air flow path 1210 and the feeding path 1022 to be simultaneously conducted when adding the sample, thereby better balancing the air pressure in the sample chamber 211 with the external air pressure. Thereby preferably facilitating sample addition.
The present invention and its embodiments have been described above schematically, without limitation, and what is shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. Therefore, if the person skilled in the art receives the teaching, without departing from the spirit of the invention, the person skilled in the art shall not inventively design the similar structural modes and embodiments to the technical solution, but shall fall within the scope of the invention.
Claims (6)
1. Detection system based on micro-fluidic technique, its characterized in that: the device comprises a high-pressure air source mechanism, a sample introduction mechanism (200), a feeding mechanism (1100) and a microfluidic chip, wherein the feeding mechanism (1100) is used for adding a sample into the sample introduction mechanism (200), the high-pressure air source mechanism is used for providing a high-pressure air source for the sample introduction mechanism (200) so as to press the sample in the sample introduction mechanism (200) into the microfluidic chip, and the microfluidic chip is used for analyzing and processing the sample;
the sample feeding mechanism (200) comprises a cylindrical sample feeding mechanism main body (210), and a plurality of independent sample cavities (211) are arranged in the sample feeding mechanism main body (210); the sample cavity (211) extends inwards from the lower end surface of the sample feeding mechanism main body (210) along the axial direction, and the lower end of the sample feeding mechanism main body (210) is provided with a sealing bottom plate (220) used for sealing the sample cavity (211); the middle part of the upper end surface of the sample feeding mechanism main body (210) extends inwards to form an air inlet cavity (212), the upper end of the sample feeding mechanism main body (210) is provided with a sealing cover plate (230) for sealing the air inlet cavity (212), and the sealing cover plate (230) is provided with an air inlet (231) for connecting with a high-pressure air source mechanism;
an air flow channel (213) is arranged between the air inlet cavity (212) and any sample cavity (211) along the radial direction, an air valve mounting hole (214) for mounting an air valve mechanism (300) is axially arranged at the position of the upper end surface of the sample injection mechanism main body (210) corresponding to any air flow channel (213), and the air valve mechanism (300) is used for adjusting the effective air inlet cross-sectional area of the air flow channel (213); the upper end surface of the sample injection mechanism main body (210) is axially provided with a sample injection port (215) communicated with the sample cavity (211), and the sample injection port (215) is used for being connected with the feeding mechanism (1100); a sample outlet (216) communicated with the sample cavity (211) is radially arranged on the side surface of the sample injection mechanism main body (210), and the sample outlet (216) is used for being connected with the microfluidic chip;
a feeding valve (1000) is arranged at the sample inlet (215), the feeding valve (1000) comprises a feeding valve seat (1010), and a feeding valve core mounting hole (1011) is formed in the feeding valve seat (1010) in a penetrating manner along the axial direction; a feeding valve core (1020) is slidably arranged in the feeding valve core mounting hole (1011), and the feeding valve core (1020) is in sealing fit with the feeding valve core mounting hole (1011); a feeding cavity (1021) with an upward opening is arranged in the feeding valve core (1020) along the axial direction, and a feeding channel (1022) communicated with the feeding cavity (1021) is arranged on the side wall of the lower part of the feeding valve core (1020) along the radial direction; the middle part of the feeding valve core (1020) extends towards the outer periphery to form a feeding valve core blocking ring part (1023), a first spring cavity (1012) which is in clearance fit with the valve core blocking ring part (1023) is arranged at the upper end of the valve core mounting hole (1011), and a first spring (1013) which is sleeved at the feeding valve core (1020) is arranged between the bottom wall of the first spring cavity (1012) and the valve core blocking ring part (1023); a feeding valve core limiting ring (1014) is arranged at an opening of the first spring cavity (1012), and the feeding valve core limiting ring (1014) is used for limiting the ascending position of the feeding valve core (1020); the feeding channel (1022) is positioned in the feeding valve core mounting hole (1011) when the feeding valve core (1020) is in the upper limit position, and the feeding channel (1022) is positioned below the feeding valve core mounting hole (1011) when the feeding valve core (1020) is in the lower limit position;
the feeding mechanism (1100) comprises a feeding mechanism main body (1110), a sample storage cavity (1111) is arranged in the feeding mechanism main body (1110), and a sample adding port (1112) communicated with the sample storage cavity (1111) is arranged at the upper end of the feeding mechanism main body (1110); a discharge valve mounting groove (1113) is formed in the lower end face of the feeding mechanism main body (1110), and a discharge valve core mounting hole (1114) communicated with the sample storage cavity (1111) is formed in the middle of the bottom wall of the discharge valve mounting groove (1113); a discharge valve core (1120) is arranged in the discharge valve core mounting hole (1114), a discharge cavity (1121) with an opening facing the sample storage cavity (1111) is axially arranged in the discharge valve core (1120), and a discharge channel (1122) communicated with the discharge cavity (1121) is radially arranged on the side wall of the lower part of the discharge valve core (1120); a discharge channel sealing ring (1130) is slidably sleeved at the discharge valve core (1120), and the discharge channel sealing ring (1130) is in sealing fit with the discharge valve core (1120); a second spring (1140) is arranged between the discharge channel sealing ring (1130) and the bottom wall of the discharge valve mounting groove (1113), and a sealing ring limiting ring (1150) used for limiting the descending position of the discharge channel sealing ring (1130) is arranged at the position, below the discharge channel sealing ring (1130), of the discharge valve mounting groove (1113); when the discharge channel sealing ring (1130) is located at the lower limit position, the discharge channel (1122) is sealed by the discharge channel sealing ring (1130), and when the discharge channel sealing ring (1130) is located at the upper limit position, the discharge channel (1122) is located below the discharge channel sealing ring (1130); the upper end of the feeding valve core limiting ring (1014) is provided with a limiting ring extrusion part (1014a) used for being matched with the discharging channel sealing ring (1130), and the diameter of an inner hole of the sealing ring limiting ring (1150) is larger than the outer diameter of the limiting ring extrusion part (1014 a).
2. The microfluidic technology-based detection system of claim 1, wherein: the cross section of the air flow channel (213) is square, a valve core movable hole (311) with a rectangular cross section is formed at the lower part of the air valve mounting hole (214), and a valve seat mounting hole (312) with a circular cross section is formed at the upper part of the air valve mounting hole (214); the length direction of the cross section of the valve core movable hole (311) is vertical to the extension direction of the air flow channel (213), and the valve core movable hole (311) and the corresponding inner wall of the air flow channel (213) are on the same vertical plane; the valve core (320) is movably arranged in the valve core movable hole (311) up and down, the valve core (320) is hermetically matched with the valve core movable hole (311) and the air flow channel (213), and the heights of the valve core (320), the valve core movable hole (311) and the air flow channel (213) in the vertical direction are the same; a valve rod (330) is vertically arranged above the valve core (320), a valve rod fixing ring (340) is arranged at the bottom of the valve seat mounting hole (312), a valve rod guide hole (341) used for sliding fit of the valve rod (330) is formed in the middle of the valve rod fixing ring (340), and the valve rod (330) is in sealing fit with the valve rod guide hole (341); the valve seat mounting hole (312) is located and is equipped with bearing mounting ring (350) in valve rod fixed ring (340) top department, and bearing mounting ring (350) hole department is equipped with bearing (360), and bearing (360) hole department is equipped with adjustable ring (370), and adjustable ring (370) hole and valve rod (330) screw-thread fit.
3. The microfluidic technology based detection system of claim 2, wherein: the thread lead of the adjusting ring (370) is equal to the height of the valve core (320) in the vertical direction.
4. The microfluidic technology based detection system of claim 3, wherein: the upper surface of the adjusting ring (370) is uniformly provided with a plurality of scale marks (410) along the circumferential direction, the side surface of the adjusting ring (370) is provided with a positioning bayonet (430) corresponding to any scale mark (410), a positioning mark block (420) matched with the positioning bayonet (430) is arranged at the sampling mechanism main body (210), and the positioning mark block (420) is detachably inserted at the sampling mechanism main body (210).
5. The microfluidic technology based detection system of claim 4, wherein: an adjusting ring mounting part (510) matched with the bearing (360) is formed at the lower end of the adjusting ring (370), an adjusting ring deflector part (520) is formed at the upper end of the adjusting ring (370), and the positioning bayonet (430) is arranged at the adjusting ring deflector part (520).
6. The microfluidic technology based detection system of claim 5, wherein: the valve core (320) and the valve rod (330) are of an integral structure.
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