CN110227564A - Detection system based on microflow control technique - Google Patents
Detection system based on microflow control technique Download PDFInfo
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- CN110227564A CN110227564A CN201910521739.XA CN201910521739A CN110227564A CN 110227564 A CN110227564 A CN 110227564A CN 201910521739 A CN201910521739 A CN 201910521739A CN 110227564 A CN110227564 A CN 110227564A
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- 238000001514 detection method Methods 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000007789 sealing Methods 0.000 claims description 22
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 7
- 210000003813 thumb Anatomy 0.000 claims description 6
- 238000007373 indentation Methods 0.000 abstract description 3
- 238000007599 discharging Methods 0.000 description 14
- 238000010079 rubber tapping Methods 0.000 description 14
- 238000010586 diagram Methods 0.000 description 13
- 239000012530 fluid Substances 0.000 description 8
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001259 photo etching Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
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Abstract
The present invention relates to microfluidic arts, specifically, being related to a kind of detection system based on microflow control technique.It includes high-pressure air source mechanism, sample introduction mechanism, feeding mechanism and micro-fluidic chip, feeding mechanism is for sample to be added in sample introduction mechanism, high-pressure air source mechanism is used to provide high-pressure air source to sample introduction mechanism so that by the sample indentation micro-fluidic chip in sample introduction mechanism, micro-fluidic chip is for being analyzed and processed sample.The configuration of the present invention is simple is convenient for operation.
Description
Technical field
The present invention relates to microfluidic arts, specifically, being related to a kind of detection system based on microflow control technique.
Background technique
Micro-fluidic (Microfluidics) is referred to using microchannel (having a size of tens of to hundreds of microns) processing or manipulation
Science and Technology involved in the system of minute fluid (volume be nanoliter arrive A Sheng) is one and is related to chemistry, fluid physics, micro-
The emerging cross discipline of electronics, new material, biology and biomedical engineering.Because have many characteristics, such as micromation, it is integrated, it is micro-
Flow control apparatus is commonly known as micro-fluidic chip, also referred to as chip lab (Lab on a Chip) and micro-total analysis system
(micro-TotalAnalytical System).Micro-fluidic early stage concept can trace back to 19 century 70s using photoetching
The gas chromatograph that technology makes on silicon wafer, then development is microcurrent controlled capillary tube electrophoresis instrument and microreactor etc. again.Miniflow
The important feature of control first is that having unique fluid properties, such as laminar flow and drop under micro-scale environments.By these uniquenesses
Fluid phenomenon, it is micro-fluidic a series of conventional methods may be implemented to be difficult to the micro Process completed and microoperation.Currently, micro-fluidic
It is considered that there is huge development potentiality and broad application prospect in biomedical research
Microfluidic system the operation such as is prepared, reacts, separates, detects to fluid in micro-nano-scale space.It is micro-fluidic
System mainly includes micro-fluidic chip, fluid path sampling system and detection system.Fluid path sampling system is micro- for injecting a sample into
It is analyzed on the micro-fluidic chip of sight.For now, existing fluid path sampling system structure and complicated for operation.
Summary of the invention
The contents of the present invention are to provide a kind of detection system based on microflow control technique, can overcome certain of the prior art
Kind or certain defects.
Detection system according to the present invention based on microflow control technique comprising high-pressure air source mechanism, sample introduction mechanism, charging
Mechanism and micro-fluidic chip, for sample to be added in sample introduction mechanism, high-pressure air source mechanism is used for sample introduction machine feeding mechanism
Structure provides high-pressure air source so that by the sample indentation micro-fluidic chip in sample introduction mechanism, micro-fluidic chip is for dividing sample
Analysis processing；
Sample introduction mechanism includes columned sample introduction mechanism main body, is equipped with multiple independent sample cavities in sample introduction mechanism main body；
Sample cavity is inwardly in the axial direction from sample introduction mechanism lower body face, and sample introduction mechanism lower body is equipped with for carrying out to sample cavity
The seal base of sealing；Inlet chamber is extended inwardly to form in the middle part of sample introduction mechanism main body upper surface, sample introduction mechanism main body upper end is equipped with
Seal cover board for being sealed to inlet chamber is equipped with the air inlet for connecting with high-pressure air source mechanism at seal cover board；
Air flow channel is radially equipped between inlet chamber and any sample cavity, sample introduction mechanism main body upper surface corresponds to any
Along the air valve mounting hole being axially arranged with for installing valve train at air flow channel, valve train is for there is air flow channel
Effect intake area is adjusted；Sample introduction mechanism main body upper surface edge is axially arranged with the injection port being connected to sample cavity, sample introduction
Mouth with feeding mechanism for connecting；Sample introduction mechanism body side is radially equipped with the outlet being connected to sample cavity, and outlet is used
It is connect in micro-fluidic chip.
In detection system of the invention, high-pressure air source provided by high-pressure air source mechanism can preferably will be in sample cavity
Sample be pressed into micro-fluidic chip in, due to sample cavity can be arranged it is multiple, while so as to preferably realize different samples
Sample introduction, so that structure is simple, it is easy to operate.In addition, due to the setting of valve train, it can be preferably to the effective of air flow channel
Intake area is adjusted, and then preferably the charge flow rate in each sample cavity can be adjusted, and then can
Preferably the sample introduction pressure of each sample cavity is adjusted, so as to preferably meet different sample volumes sample room it is same
When sample introduction.
Preferably, the cross section of air flow channel is square, it is rectangular that cross section, which is formed, at air valve mounting hole lower part
Spool movable span, it is circular valve seat mounting hole that cross section, which is formed, at air valve mounting hole top；The length of spool movable span cross section
Direction is vertical with the extending direction of air flow channel, and spool movable span is with the corresponding inner wall of air flow channel on same perpendicular；
Spool, spool and spool movable span and air flow channel close fit, spool, valve can be equipped in spool movable span up and downly
The height of core movable span and air flow channel in the vertical direction is identical；Valve rod, valve seat mounting hole bottom are equipped with above spool vertically
Portion is equipped with valve rod fixed ring, and the stem guiding hole for valve stem slides cooperation is equipped in the middle part of valve rod fixed ring, and valve rod is led with valve rod
It seals and cooperates to hole；Valve seat mounting hole is equipped with bearing mounting ring at being located above valve rod fixed ring, sets at bearing mounting ring inner hole
There is bearing, be equipped at brearing bore and adjust ring, adjusts ring inner hole and threaded stem cooperates.
In detection system of the invention, by bearing mounting ring, bearing and the cooperation for adjusting ring, so that being adjusted by adjusting
Section ring can preferably be adjusted the lifting distance of spool；Again since the cross section of air flow channel is square, to make
The lifting distance for obtaining spool can be directly proportional to the conducting area of air flow channel, so as to preferably to the conducting of air flow channel
Area is adjusted；Again since spool, the height of spool movable span and air flow channel in the vertical direction are identical, so that
Spool in that upper limit position Airflow road is fully on, spool is in lower position Airflow road is completely switched off, thus
It is adjusted convenient for the conducting area to air flow channel.
Preferably, the lead of screw thread for adjusting ring is equal to the height of spool in the vertical direction.So that adjusting ring rotation
It circles, spool can be realized, the fully on of air flow channel is completely enclosed to air flow channel, thus by adjusting ring
The control of rotation angle can preferably realize the control to distance above and below spool, and due to the conducting area and valve of air flow channel
Core up and down apart from directly proportional, and then can preferably realize relatively precisely adjusting to air flow channel conducting area, and then can
It is adjusted preferably according to the conducting area of the sample introduction comparison air flow channel of different samples.
Preferably, adjusting ring upper surface is circumferentially uniformly provided with a plurality of graduation mark, adjusts ring side and correspond to any scale
It is equipped with limited bayonet at line, the locative marking block for cooperating with limited bayonet, telltale mark are equipped at sample introduction mechanism main body
Block is detachably inserted at sample introduction mechanism main body.By the setting of a plurality of graduation mark, more can accurately realize to adjusting
The adjusting of ring rotation angle.In addition, passing through the design of limited bayonet and locative marking block, can not only preferably realize to adjusting
Ring rotates the label of angle, and can also effectively realize the fixation to ring rotation position is adjusted.Wherein, locative marking block is cross
Section is the cylinder of equilateral triangle, and the equilateral triangle jack for locative marking block insertion is equipped at sample introduction mechanism main body, thus
So that preferably adjusting ring can be adjusted, insertion locative marking block can be realized preferably after extracting locative marking block
Fixation to ring is adjusted.
It is formed preferably, adjusting ring lower end for the adjusting ring mounting portion with bearing fit, adjusts ring upper end and form tune
Ring thumb wheel portion is saved, limited bayonet, which is set to, to be adjusted at ring thumb wheel portion.So as to preferably facilitate the installation of adjusting ring and stir.
Preferably, spool is structure as a whole with valve rod.Consequently facilitating the manufacture of spool and valve rod.
Detailed description of the invention
Fig. 1 is the system block diagram schematic diagram of the detection system in embodiment 1；
Fig. 2 is the schematic diagram of the sample introduction mechanism in embodiment 1；
Fig. 3 is the schematic diagram of the valve train in embodiment 1；
Fig. 4 bows for the valve train in embodiment 1 to schematic diagram；
Fig. 5 is the schematic diagram of the adjusting ring in embodiment 1；
Fig. 6 is the schematic diagram of the spool and valve rod in embodiment 1；
Fig. 7 is the schematic diagram of the sample introduction mechanism main body in embodiment 1；
Fig. 8 is the half-section diagram of the sample introduction mechanism main body in embodiment 1；
Fig. 9 is the schematic diagram of the seal base in embodiment 1；
Figure 10 is the schematic diagram of the inlet valve in embodiment 1；
Figure 11 is the schematic diagram of the feeding mechanism in embodiment 1；
Figure 12 is the schematic diagram of the charging spool in embodiment 1.
Specific embodiment
To further appreciate that the contents of the present invention, the present invention is described in detail in conjunction with the accompanying drawings and embodiments.It should be understood that
, embodiment be only to the present invention explain and and it is non-limiting.
Embodiment 1
As shown in connection with fig. 1, a kind of detection system based on microflow control technique is present embodiments provided comprising high-pressure air source
Mechanism, sample introduction mechanism 200, feeding mechanism 1100 and micro-fluidic chip, feeding mechanism 1100 are used to sample being added to sample introduction machine
In structure 200, high-pressure air source mechanism is used to provide high-pressure air source to sample introduction mechanism 200 so that the sample in sample introduction mechanism 200 to be pressed into
In micro-fluidic chip, micro-fluidic chip is for being analyzed and processed sample.
As shown in Fig. 2, sample introduction mechanism 200 includes columned sample introduction mechanism main body 210, set in sample introduction mechanism main body 210
There are multiple independent sample cavities 211；Sample cavity 211 is inwardly in the axial direction from 210 lower end surface of sample introduction mechanism main body, sample introduction mechanism
210 lower end of main body is equipped with the seal base 220 for being sealed to sample cavity 211；In the middle part of 210 upper surface of sample introduction mechanism main body
Inlet chamber 212 is extended inwardly to form, 210 upper end of sample introduction mechanism main body is equipped with the sealing cover for being sealed to inlet chamber 212
Plate 230 is equipped with the air inlet 231 for connecting with high-pressure air source mechanism at seal cover board 230；
Air flow channel 213 is radially equipped between inlet chamber 212 and any sample cavity 211, in sample introduction mechanism main body 210
End face corresponds at any air flow channel 213 along the air valve mounting hole 214 being axially arranged with for installing valve train 300, air valve
Mechanism 300 is for being adjusted the effective admission area of section of air flow channel 213；210 upper surface of sample introduction mechanism main body is along axis
To the injection port 215 being connected to sample cavity 211 is equipped with, injection port 215 with feeding mechanism 1100 for connecting；Sample introduction mechanism main body
210 sides are radially equipped with the outlet 216 being connected to sample cavity 211, and outlet 216 with micro-fluidic chip for connecting.
In the detection system of the present embodiment, high-pressure air source provided by high-pressure air source mechanism can be preferably by sample cavity
In 211 sample indentation micro-fluidic chip in, due to sample cavity 211 can be arranged it is multiple, so as to preferably realize difference
Sample introduction while sample, so that structure is simple, it is easy to operate.In addition, due to the setting of valve train 300, it can be preferably right
The effective admission area of section of air flow channel 213 is adjusted, and then can be preferably to the inlet air flow in each sample cavity 211
Amount is adjusted, so can the sample introduction pressure preferably to each sample cavity 211 be adjusted, so as to preferably meet
The sample introduction while sample room of different sample volumes.
As shown in connection with fig. 3, the cross section of air flow channel 213 is square, and 214 lower part of air valve mounting hole forms cross section and is
Rectangular spool movable span 311, it is circular valve seat mounting hole 312 that cross section, which is formed, at 214 top of air valve mounting hole；Spool is living
The length direction of dynamic 311 cross section of hole is vertical with the extending direction of air flow channel 213, spool movable span 311 and air flow channel 213
Correspondence inner wall on same perpendicular；Spool 320, spool 320 and valve can be equipped in spool movable span 311 up and downly
213 close fit of core movable span 311 and air flow channel, spool 320, spool movable span 311 and air flow channel 213 are vertical
Height on direction is identical；320 top of spool is equipped with valve rod 330 vertically, and 312 bottom of valve seat mounting hole is equipped with valve rod fixed ring
340, the stem guiding hole 341 being slidably matched for valve rod 330, valve rod 330 and stem guiding are equipped in the middle part of valve rod fixed ring 340
The sealing cooperation of hole 341；Valve seat mounting hole 312, which is located above valve rod fixed ring 340, is equipped with bearing mounting ring 350, bearing installation
It is equipped with bearing 360 at 350 inner hole of ring, is equipped at 360 inner hole of bearing and adjusts ring 370, adjust 370 inner hole of ring and 330 screw thread of valve rod
Cooperation.
In the detection system of the present embodiment, by bearing mounting ring 350, bearing 360 and the cooperation for adjusting ring 370, make
By adjust adjusting ring 370 can the lifting distance preferably to spool 320 be adjusted；Again due to air flow channel 213
Cross section is square, so that the lifting distance of spool 320 can be directly proportional to the conducting area of air flow channel 213, from
And can the conducting area preferably to air flow channel 213 be adjusted；Again due to spool 320, spool movable span 311 and sky
The height of flow channel 213 in the vertical direction is identical, so that spool 320 is complete in upper limit position Airflow road 213
Full conducting, that spool 320 is in lower position Airflow road 213 is completely switched off, consequently facilitating to the conducting face of air flow channel 213
Product is adjusted.
In the present embodiment, the lead of screw thread for adjusting ring 370 is equal to the height of spool 320 in the vertical direction.So that
It adjusts ring 370 to rotate a circle, spool 320 can be realized, leading completely for air flow channel 213 is completely enclosed to air flow channel 213
It is logical, thus by can preferably realize the control to about 320 distance of spool to the control for adjusting the rotation angle of ring 370, again
Due to air flow channel 213 conducting area and spool 320 up and down apart from directly proportional, and then can preferably realize to air stream
Relatively precisely adjusting for area is connected in road 213, and then can compare leading for air flow channel 213 preferably according to the sample introduction of different samples
Logical area is adjusted.
As shown in connection with fig. 4, it adjusts 370 upper surface of ring and is circumferentially uniformly provided with a plurality of graduation mark 410, adjust 370 side of ring
It is equipped with limited bayonet 430 at corresponding any graduation mark 410, is equipped at sample introduction mechanism main body 210 for matching with limited bayonet 430
The locative marking block 420 of conjunction, locative marking block 420 are detachably inserted at sample introduction mechanism main body 210.Pass through a plurality of graduation mark
410 setting more can accurately realize the adjusting to the rotation angle of ring 370 is adjusted.In addition, by limited bayonet 430 with
The design of locative marking block 420 can not only preferably realize the label that angle is rotated to adjusting ring 370, and can also be effective
Realize the fixation to 370 rotation position of ring is adjusted in ground.Wherein, locative marking block 420 is the cylinder that cross section is equilateral triangle, into
The equilateral triangle jack being inserted into for locative marking block 420 is equipped at sample mechanism body 210, so that extracting locative marking block
After 420, preferably adjusting ring 370 can be adjusted, insertion locative marking block 420 can be realized preferably to adjusting ring
370 fixation.
As shown in connection with fig. 5, adjusting ring mounting portion 510 of 370 lower end of the ring formation for cooperating with bearing 360 is adjusted, is adjusted
370 upper end of ring forms the ring thumb wheel portion 520 that adjusts, and limited bayonet 430, which is set to, to be adjusted at ring thumb wheel portion 520.So as to preferably
Convenient for adjusting the installation of ring 370 and stirring.
As shown in connection with fig. 6, spool 320 is structure as a whole with valve rod 330.Consequently facilitating the system of spool 320 and valve rod 330
It makes.
It in conjunction with Fig. 7 and Fig. 8, is upwardly extended on the outside of 211 upper end of sample cavity, forms sample cavity buffer part 211a, air flow channel
213 and injection port 215 be directly connected to sample cavity buffer part 211a, gentle flowed into so as to preferably facilitate sample addition
Enter.In addition, 212 opening of inlet chamber forms the seal cover board mounting groove 810 for installing seal cover board 230, consequently facilitating close
Cover the setting of plate 230；210 lower end surface of sample introduction mechanism main body forms the seal base mounting groove for installing seal base 220
820, consequently facilitating the installation of seal base 220.Constructed by this kind of sample introduction mechanism main body 210, can preferably facilitate into
The die sinking of sample mechanism body 210, and then convenient for manufacture.
As shown in connection with fig. 9, seal base 220 includes bottom plate 910, and the diameter of bottom plate 910 is straight with sample introduction mechanism main body 210
Diameter is identical；The seal base erecting bed 920 for cooperating with seal base mounting groove 820, seal base are equipped with above bottom plate 910
It is equipped at the corresponding any sample cavity 211 of erecting bed 920 for the sample cavity plug with the sealing cooperation of 211 lower ending opening of sample cavity
Portion 930, so as to preferably guarantee the airtightness of sample cavity 211；930 upper surface of any sample cavity plug portion is equipped with orthogonal
2 sample diversion trenches 940 being arranged, the one in 2 sample diversion trenches 940 are set along the radial direction of sample introduction mechanism main body 210
It sets, the position of outlet 216 is corresponding with the sample diversion trench 940 that this is arranged radially, so as to preferably facilitate sample
Outflow.
High-pressure air source mechanism, sample introduction mechanism 200 and feeding mechanism 1100 in the present embodiment can be separately formed sample into
Sample system can preferably apply in a variety of microfluidic systems.
As shown in connection with fig. 10, inlet valve 1000 is equipped at injection port 215, inlet valve 1000 includes charging valve seat 1010, into
Expect to be equipped with charging spool mounting hole 1011 axially through ground in valve seat 1010；It is slideably set in charging spool mounting hole 1011
There is charging spool 1020, charging spool 1020 and the charging sealing of spool mounting hole 1011 cooperate；It feeds in spool 1020 along axial direction
Feed cavity 1021 equipped with opening upwards, charging 1020 lower sides of spool are radially equipped with the charging being connected to feed cavity 1021
Channel 1022；It extends outside to form charging spool baffle ring portion 1023,1011 upper end of spool mounting hole in the middle part of charging spool 1020
Equipped with for the first spring cavity 1012 with 1023 clearance fit of spool baffle ring portion, 1012 bottom wall of the first spring cavity and spool baffle ring
The first spring 1013 being sheathed at charging spool 1020 is equipped between portion 1023；First spring cavity, 1012 opening is equipped with charging
Spool stop collar 1014, charging spool stop collar 1014 are used to limit the upward position of charging spool 1020；Charging is logical
Road 1022 is located in charging spool mounting hole 1011 when feeding spool 1020 and being in upper limit position, and feeding-passage 1022 is being fed
Spool 1020 is located at 1011 lower section of charging spool mounting hole when being in lower position.
In the sample feeding system of the present embodiment, when needing sample introduction, feeding mechanism 1100 can be to charging spool 1020
It is squeezed, so that feed cavity 1021 and sample cavity 211 is connected in feeding-passage 1022, so that sample can be preferably
It is added into sample cavity 211；After completing sample introduction, under the action of the first spring 1013, charging spool 1020 can be protected always
Upper limit position is held, so as to preferably separate feed cavity 1021 and sample cavity 211, and then convenient for sample in sample cavity 211
It is forced out.
In conjunction with shown in Figure 11, feeding mechanism 1100 includes feeding mechanism main body 1110, is equipped in feeding mechanism main body 1110
Sample storage chamber 1111,1110 upper end of feeding mechanism main body are equipped with the sample adding mouth 1112 being connected to sample storage chamber 1111；
1110 lower end surface of feeding mechanism main body is equipped with outlet valve mounting groove 1113, and 1113 bottom wall of outlet valve mounting groove middle part is equipped with and sample
The discharging spool mounting hole 1114 that storage chamber 1111 is connected to；The spool mounting hole 1114 that discharges is interior to be equipped with discharging spool 1120, discharging
Along discharging chamber 1121 of the opening towards sample storage chamber 1111 is axially arranged in spool 1120, discharge 1120 lower sides edge of spool
It is radially provided with the tapping channel 1122 being connected to discharging chamber 1121；It is close that a tapping channel is slideably arranged at discharging spool 1120
Seal ring 1130, tapping channel sealing ring 1130 and the discharging sealing of spool 1120 cooperate；Tapping channel sealing ring 1130 and outlet valve
Second spring 1140 is equipped between 1113 bottom wall of mounting groove, outlet valve mounting groove 1113 is located at below tapping channel sealing ring 1130
Equipped with the sealing ring stop collar 1150 for being limited 1130 downstream position of tapping channel sealing ring；Tapping channel sealing ring
1130 be located at lower position when tapping channel 1122 sealed by tapping channel sealing ring 1130, tapping channel sealing ring 1130 is located at
Tapping channel 1122 is located at 1130 lower section of tapping channel sealing ring when upper limit position；It feeds 1014 upper end of spool stop collar and forms use
In the stop collar press section 1014a cooperated with tapping channel sealing ring 1130, the diameter of bore of sealing ring stop collar 1150
Greater than the outer diameter of stop collar press section 1014a.
It, can be by the discharging insertion charging of spool 1120 when needing to add sample in the sample feeding system of the present embodiment
In the feed cavity 1021 of spool 1020, then push feeding mechanism 1100 so that charging spool 1020 can downlink until
Feed cavity 1021 and sample cavity 211 is connected, with continuing traveling downwardly for feeding mechanism 1100, stop collar press section 1014a can be right
Sealing ring 1130 carry out squeeze until tapping channel 1122 be exposed to sealing ring 1130, can preferably realize adding for sample at this time
Add.
In the sample feeding system of the present embodiment, discharging 1120 lower end of spool is shunk in outlet valve mounting groove 1113.From
And feeding mechanism 1100 is preferably uprightly placed.
In the sample feeding system of the present embodiment, charging spool stop collar 1014 is threadedly engaged with the first spring cavity 1012,
The middle part of charging spool stop collar 1014 extends outside to form charging spool stop collar rib portion 1014b.So as to preferable
It is convenient for the installation of charging spool stop collar 1014 in ground.
In the sample feeding system of the present embodiment, extend outside to be formed for pacifying with outlet valve in the middle part of the spool 1120 that discharges
The discharging spool rib portion 1123 of 1113 bottom wall of tankage cooperation, second spring 1140 are located at discharging spool rib portion 1123 and discharging
Between channel sealing ring 1130.From the installation that can preferably facilitate discharging spool 1120.
In the sample feeding system of the present embodiment, charging spool 1020 and discharging spool 1120 are structure as a whole.To
Manufacture easy to produce.
It in conjunction with shown in Figure 12, feeds and is equipped with air flow channel 1210 at spool 1020,1210 upper and lower opening of air flow channel is all provided with
In charging 1020 lateral position of spool, the axis of the under shed of air flow channel 1210 and the axis of feeding-passage 1022 are in same level
On face, 1210 upper opening of air flow channel is always positioned at 1014 top of charging spool stop collar.This makes when adding sample, air
Runner 1210 can be simultaneously turned on feeding-passage 1022, so as to better balance sample cavity 211 and extraneous air pressure.
And then sample addition can be preferably facilitated.
Schematically the present invention and embodiments thereof are described above, description is not limiting, institute in attached drawing
What is shown is also one of embodiments of the present invention, and actual structure is not limited to this.So if the common skill of this field
Art personnel are enlightened by it, without departing from the spirit of the invention, are not inventively designed and the technical solution
Similar frame mode and embodiment, are within the scope of protection of the invention.
Claims (6)
1. the detection system based on microflow control technique, it is characterised in that: including high-pressure air source mechanism, sample introduction mechanism (200), charging
Mechanism (1100) and micro-fluidic chip, feeding mechanism (1100) is for sample to be added in sample introduction mechanism (200), high-pressure air source
Mechanism is used to provide high-pressure air source to sample introduction mechanism (200) so that the sample in sample introduction mechanism (200) to be pressed into micro-fluidic chip,
Micro-fluidic chip is for being analyzed and processed sample；
Sample introduction mechanism (200) includes columned sample introduction mechanism main body (210), is equipped in sample introduction mechanism main body (210) multiple only
Vertical sample cavity (211)；Sample cavity (211) is inwardly in the axial direction from sample introduction mechanism main body (210) lower end surface, sample introduction mechanism master
Body (210) lower end is equipped with the seal base (220) for being sealed to sample cavity (211)；Sample introduction mechanism main body (210) upper end
Middle face extends inwardly to form inlet chamber (212), and sample introduction mechanism main body (210) upper end is equipped with for carrying out to inlet chamber (212)
The seal cover board (230) of sealing is equipped with the air inlet (231) for connecting with high-pressure air source mechanism at seal cover board (230)；
Air flow channel (213) radially are equipped between inlet chamber (212) and any sample cavity (211), sample introduction mechanism main body
(210) upper surface corresponds at any air flow channel (213) along the air valve installation being axially arranged with for installing valve train (300)
Hole (214), valve train (300) is for being adjusted the effective admission area of section of air flow channel (213)；Sample introduction mechanism master
Body (210) upper surface is along the injection port (215) being connected to sample cavity (211) is axially arranged with, and injection port (215) is used for and charger
Structure (1100) connection；Sample introduction mechanism main body (210) side is radially equipped with the outlet (216) being connected to sample cavity (211), out
Sample mouth (216) with micro-fluidic chip for connecting.
2. the detection system according to claim 1 based on microflow control technique, it is characterised in that: air flow channel (213)
Cross section is square, and it is rectangular spool movable span (311), air valve peace that cross section, which is formed, at air valve mounting hole (214) lower part
Filling hole (214) top and forming cross section is circular valve seat mounting hole (312)；The length side of spool movable span (311) cross section
To vertical with the extending direction of air flow channel (213), spool movable span (311) is with the corresponding inner wall of air flow channel (213) same
On one perpendicular；Spool (320) can be equipped with up and downly in spool movable span (311), spool (320) and spool movable span
(311) and air flow channel (213) close fit, spool (320), spool movable span (311) and air flow channel (213) are erecting
The upward height of histogram is identical；Valve rod (330) are equipped with vertically above spool (320), valve seat mounting hole (312) bottom is equipped with valve rod
Fixed ring (340), valve rod fixed ring (340) middle part are equipped with the stem guiding hole (341) being slidably matched for valve rod (330), valve
Bar (330) and stem guiding hole (341) sealing cooperate；Valve seat mounting hole (312) is equipped at being located above valve rod fixed ring (340)
Bearing mounting ring (350) is equipped with bearing (360) at bearing mounting ring (350) inner hole, is equipped at bearing (360) inner hole and adjusts ring
(370), ring (370) inner hole is adjusted to be threadedly engaged with valve rod (330).
3. the detection system according to claim 2 based on microflow control technique, it is characterised in that: adjust the spiral shell of ring (370)
Line lead is equal to the height of spool (320) in the vertical direction.
4. the detection system according to claim 3 based on microflow control technique, it is characterised in that: adjust table on ring (370)
Face is circumferentially uniformly provided with a plurality of graduation mark (410), is adjusted ring (370) side and is corresponded to be equipped at any graduation mark (410) and determines
Position bayonet (430) is equipped with the locative marking block (420) for cooperating with limited bayonet (430) at sample introduction mechanism main body (210),
Locative marking block (420) is detachably inserted at sample introduction mechanism main body (210).
5. the detection system according to claim 4 based on microflow control technique, it is characterised in that: adjust ring (370) lower end
The adjusting ring mounting portion (510) for cooperating with bearing (360) is formed, ring (370) upper end is adjusted and forms adjusting ring thumb wheel portion
(520), limited bayonet (430), which is set to, is adjusted at ring thumb wheel portion (520).
6. the detection system according to claim 5 based on microflow control technique, it is characterised in that: spool (320) and valve rod
(330) it is structure as a whole.
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