CN110196232A - A kind of confocal imaging device and confocal imaging method - Google Patents
A kind of confocal imaging device and confocal imaging method Download PDFInfo
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Abstract
The invention discloses a kind of confocal imaging device and confocal imaging methods, the embodiment of the present invention solves the problems, such as the cumbersome of scanning lighting process using aperture imaging method, and the regulatable plane reflection lens array of setting, overcome the problems, such as that confocal illumination can not elapse using the adjusting to plane reflection lens array, it realizes the underwater confocal imaging waited under application scenarios, expands application scenarios range.
Description
Technical field
The present invention relates to field of computer technology, in particular to a kind of confocal imaging device and confocal imaging method.
Background technique
It is confocal to refer to that the optical path including exciting and emitting focuses on two positions.During confocal imaging, exciting light
Sample surfaces are focused on, and emits light and focuses on illumination pin hole.This pin hole limit optical path sample surfaces the depth of focus,
Effectively prevent impurity signal reasons for its use noise jamming, to reduce the intensity of background signal, improve sample at image quality
Amount.Confocal imaging method is applied on Laser Scanning Confocal Microscope earliest, main to be created using focusing mode illumination and synchronous imaging
The cross section of three dimensional biological sample.It is a kind of smooth focus illumination and the modulation of illumination pin hole to remove dissipating for sample non-focus plane
Optical resolution and visual contrast can be improved compared to other imaging methods in the optical imagery means for penetrating light.Copolymerization is burnt aobvious
Micro mirror makes great contribution in biomedical aspect, possesses many achievements, and the substance small to research cell or brain etc. has
Great meaning.
But confocal imaging is restricted to always in microscopical design, application range is also only limited to biomedicine.For
So that the confocal imaging technology is suitable for large scale scene, proposes and used to substitute microscope using projector and camera array
Optical aperture.In specific implementation, confocal imaging technology is divided into two classes: cofocus scanning mode and confocal coding mode.Wherein, altogether
Burnt scanning mode is that the detecting pinhole of illumination pin hole and setting in front of the detector after light source unit is arranged in is utilized to realize point photograph
Bright and point detection, the light from light source unit obtain some point that transmitting light focuses on the focal plane of sample by illumination pin hole
On, which is imaged in detecting pinhole, and detector gets the image of sample by detecting pinhole.Under confocal coding mode,
Random exposure mask is set before the projector of setting, spectroscope is set before random exposure mask, makes projector and camera using spectroscope
Coaxial, the outgoing projection light source that projector issues passes through set plane after set random exposure mask and spectroscope
In the scene of lens array being reflected into where sample；After the emergent light of sample is by set planar mirror array reflection, then pass through
It crosses spectroscope to enter in camera, realizes acquisition of the camera to sample image.
But confocal imaging is carried out using above two method, all have the disadvantage that cofocus scanning mode illuminates needle due to it
Hole and the aperture of detecting pinhole are small, so areas imaging is small, and complete confocal illumination and imaging, scanning process using scanning mode
Cumbersome and speed is slow, once being applied to slightly larger scene will take a substantial amount of time and energy.Confocal coding mode overcomes
The shortcomings that cofocus scanning mode, but planar mirror array therein is that fixed setting is good, can not be adjusted, it is not easy enough flexible,
Limit the application scenarios range of confocal imaging.
Summary of the invention
In view of this, the embodiment of the present invention provides a kind of confocal imaging device, which can be easy and neatly to altogether
The illumination depth of coke imaging is adjusted, and expands the application scenarios range of confocal imaging.
The embodiment of the present invention also provides a kind of confocal imaging method, and this method can be easy and neatly to confocal imaging
Illumination depth is adjusted, and expands the application scenarios range of confocal imaging.
The embodiments of the present invention are implemented as follows:
A kind of confocal imaging device, comprising: light source unit, spectroscope, polarizing film, plane reflection lens array and imaging are single
Member, wherein plane reflection lens array is spliced to obtain by multiple rotatable plane mirrors, and the plane mirror is between any two
It is seamless and spliced plane reflection lens array have setting radian；
The white light mirror that is split of light source unit projection is transmitted on polarizing film；Polarizing film carries out polarization information to the projection light
Adjusting, the white light after the adjusting forms illuminating bundle and focuses on the focusing of sample after the reflection of plane reflection lens array
On face；
The focusing surface of sample reflects illuminating bundle, and the illuminating bundle of reflection successively passes through the anti-of plane reflection lens array
It penetrates, after the transparent transmission of polarizing film and spectroscopical reflection, sample focusing surface image is obtained by imaging unit.
Confocal imaging device as described in claim 1 is arranged in a kind of confocal imaging method, this method comprises:
The white light of light source unit projection in confocal imaging device is transmitted to confocal by the spectroscope in confocal imaging device
On polarizing film in imaging device；
White light warp after polarizing film in confocal imaging device carries out the adjusting of polarization information to the projection light, after the adjusting
After crossing the reflection of the plane reflection lens array in confocal imaging device, forms illuminating bundle and focus on the focusing surface of sample；
The focusing surface of sample reflects illuminating bundle, and the illuminating bundle of reflection successively passes through the anti-of plane reflection lens array
It penetrates, after the transparent transmission of polarizing film and spectroscopical reflection, sample focusing surface image is obtained by imaging unit.
As above it as it can be seen that the embodiment of the present invention solves the problems, such as the cumbersome of scanning lighting process using aperture imaging method, and sets
Regulatable plane reflection lens array is set, overcomes what confocal illumination can not elapse to ask using the adjusting to plane reflection lens array
Topic realizes the underwater confocal imaging waited under application scenarios, expands application scenarios range.
Detailed description of the invention
Fig. 1 is confocal imaging apparatus structure schematic diagram provided in an embodiment of the present invention；
Fig. 2 is confocal imaging method flow chart provided in an embodiment of the present invention；
Fig. 3 is confocal imaging device specific example structural schematic diagram provided in an embodiment of the present invention；
Fig. 4 is illumination provided in an embodiment of the present invention and imaging optical path schematic diagram；
Fig. 5 is confocal passage conceptual schematic view provided in an embodiment of the present invention.
Label declaration
11- light source unit
12- spectroscope
13- polarizing film
14- plane reflection lens array
15- imaging unit
31- projector
32- camera
33- sample
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention more comprehensible, right hereinafter, referring to the drawings and the embodiments,
The present invention is further described.
Although the confocal coding mode in background technique can expand areas imaging, and due to not needing to complete using scanning
Confocal illumination, so that confocal imaging process is simple and speed is fast.But due to the planar mirror array in confocal coding mode
It is that fixed setting is good, can not adjusts, it is not easy enough flexible, limit the application scenarios of confocal imaging.Therefore, the present invention is implemented
In order to solve this problem, using aperture imaging method example solves the problems, such as the cumbersome of scanning lighting process, and be arranged regulatable
Plane reflection lens array overcomes the problems, such as that confocal illumination can not elapse using the adjusting to plane reflection lens array, realizes such as
The underwater confocal imaging waited under application scenarios, expands application scenarios range.
Fig. 1 is confocal imaging apparatus structure schematic diagram provided in an embodiment of the present invention, comprising: light source unit 11, spectroscope
12, polarizing film 13, plane reflection lens array 14 and imaging unit 15, wherein plane reflection lens array 14 is by multiple rotatable
Plane mirror splices to obtain, and the plane mirror is seamless between any two and has in spliced plane reflection lens array 14
There is setting radian.
The white light mirror 12 that is split that light source unit 11 projects is transmitted on polarizing film 13；Polarizing film 13 carries out the projection light
After the adjusting of polarization information, the white light after the adjusting forms illuminating bundle and focuses after the reflection of plane reflection lens array 14
In on the focusing surface of sample；
The focusing surface of sample reflects illuminating bundle, and the illuminating bundle of reflection successively passes through the anti-of plane reflection lens array 14
It penetrates, after the reflection of the transparent transmission of polarizing film 13 and spectroscope 14, sample focusing surface image is obtained by imaging unit 15.
In embodiments of the present invention, the white light that the light source unit 11 is projected have passed through the coding of the projected image of setting
Afterwards, then the mirror 12 that is split receives.
In embodiments of the present invention, the polarizing film 13 to the projection light carry out polarization information be adjusted to horizontal polarization and
The adjusting of vertical polarization；The projected image of the setting includes: the horizontal polarization and vertical polarization according to the polarizing film 13
The Depth profile information that the sample application scenarios are calculated in information is adjusted, according to the depth distribution of the sample application scenarios
Information setting projected image.
In embodiments of the present invention, the projected image of the setting changes according to the application scenarios of the sample, is adjusted
Section updates.
In this way, can be obtained according to the polarization information of polarizing film 13 when carrying out first time confocal imaging using the equipment
The Depth profile information of the sample application scenarios is obtained, and then obtains projected image, thus in second of confocal imaging,
The projection of white light is carried out using the projected image.Judge to determine by the Depth profile information thus according to the sample application scenarios
The interesting part of invalid prospect and the sample, and the projected image being arranged, can reduce the photograph of the prospect to the sample
It is bright, to improve the interesting part of the sample and the lighting contrast ratio of invalid prospect.
In embodiments of the present invention, if replacement application scenarios, using the device adjusting projected image Luminance Distribution come
Realize adaptive confocal imaging.Specifically, adjusting or replacing projected image according to application scenarios, adaptive confocal passage is realized
Imaging.
In embodiments of the present invention, the light source unit 11 can be projector.
In embodiments of the present invention, the radian that the plane reflection lens array 14 is set is realized the white light after the adjusting is anti-
It is incident upon on the focusing surface of sample.The angle of the plane reflection lens array 14 and the radian of setting can be automatically adjusted.
In embodiments of the present invention, the spectroscope 12 is light transmission than the light splitting plain film for 50:50, setting and described device
Projection optical axis at 45 degree place；The polarizing film 13 is colored transflector polarizing film, has masking to the projection light and penetrates
Function.
In embodiments of the present invention, imaging unit 15 is charge coupled cell (CCD) plane of camera setting.
In embodiments of the present invention, further include computer, the imaging unit 15 obtain sample focusing surface image export to
Computer is handled, and identification and segmentation of sample etc. are carried out.
In embodiments of the present invention, the application scenarios of described device are underwater.
In embodiments of the present invention, the position of light source unit 11 is vertical with the position of imaging unit 15, realizes coaxial imaging.
Fig. 2 is confocal imaging method flow chart provided in an embodiment of the present invention, and confocal imaging device, specific steps are arranged
Are as follows:
The white light of light source unit projection in step 201, confocal imaging device is saturating by the spectroscope in confocal imaging device
It is mapped on the polarizing film in confocal imaging device；
After polarizing film in step 202, confocal imaging device carries out the adjusting of polarization information to the projection light, after the adjusting
White light after the reflection of the plane reflection lens array in confocal imaging device, form illuminating bundle and focus on the focusing of sample
On face；
Step 203, sample focusing surface illuminating bundle is reflected, the illuminating bundle of reflection successively passes through plane mirror battle array
After the reflection of column, the transparent transmission of polarizing film and spectroscopical reflection, sample focusing surface image is obtained by imaging unit.
In embodiments of the present invention, the plane reflection lens array is spliced to obtain by multiple rotatable plane mirrors,
The plane mirror is seamless between any two and has setting radian in spliced plane reflection lens array.The plane is anti-
The angle and setting radian for penetrating lens array are controlled to adjust.
In the method, it is transmitted in the white light by the spectroscope in confocal imaging device inclined in confocal imaging device
Before on piece of shaking, further includes: after the white light of the projection is encoded according to the projected image of setting, then connect by the spectroscope
It receives；
The described pair of projection light carries out the adjusting for being adjusted to horizontal polarization and vertical polarization of polarization information；
The projected image of the setting includes: the tune according to described pair of projection light horizontal polarization carried out and vertical polarization
The Depth profile information of the sample application scenarios is calculated in section information, is believed according to the depth distribution of the sample application scenarios
Breath setting projected image；
The projected image of the setting changes according to the application scenarios of the sample, is adjusted or updates.
In embodiments of the present invention, if replacement application scenarios, using the device adjusting projected image Luminance Distribution come
Realize adaptive confocal imaging.
As can be seen that the embodiment of the present invention uses controllable plane reflection lens array, can be controlled to adjust by outside flat
The angle of face reflection mirror array and the radian of setting overcome background technique so that illuminating bundle focuses on the focusing surface of sample
In cofocus scanning illuminate cumbersome problem, realize the confocal illumination passage of more depth.Combination aperture imaging of the embodiment of the present invention
Mode and confocal illumination driven mode solve under severe application scenarios, especially water to realize the confocal imaging of multi-angle of view
Under weak astigmatism and occlusion issue.The embodiment of the present invention uses the method that setting projected image encodes, and is projected by light source unit
White light have passed through the coding of projected image so that the white light after coding is more accurate to the irradiation of the focusing surface of sample, effectively
Different depth foreground target (for interesting target) reflection to illuminating ray is reduced, to realize sample and invalid
The high comparison illumination profile of prospect, preferably acquisition sample focusing surface image.
Fig. 3 is confocal imaging device specific example structural schematic diagram provided in an embodiment of the present invention.The device is by projector
31, spectroscope 12, polarizing film 13, plane reflection lens array 14, camera 32 and sample 33 form.Wherein spectroscope 12 selects transflection
Than for 50:50, and set the horizontal of 45 degree of placements.By the semi-transparent semi-reflecting effect of spectroscope 12, projector 31 is put
Seated position is vertical with the placement location of camera 32, and the coaxial imaging of realization, plane reflection lens array 14 are even more by 4*4 or 5*5
Adjustable size be 50 millimeters * 50 millimeters plane mirror form.By the rotation angle for adjusting each plane mirror
Degree, can make it reflect to form illuminating bundle and focus on the same focal plane of sample 33.
During carrying out confocal imaging, the light source of projector 31 is launched by A point, by the projection pattern before light source
It is encoded；Light source after being encoded is projected by spectroscope 12, and polarizing film 13 changes the polarization information of the light source after projection extremely
On plane reflection lens array 14；It is reflected at 33 place application scenarios of sample by plane reflection lens array 14 again；
Reflecting light back on plane reflection lens array 14 at 33 place application scenarios of sample, plane reflection lens array 14 will
The reflected light back reflects back on camera 32 again by polarizing film 13 and spectroscope 12, is got by camera 32；Camera
The image transmitting that will acquire carries out subsequent image procossing into computer, completes identification and segmentation of sample 33 etc..
Fig. 4 is illumination provided in an embodiment of the present invention and imaging optical path schematic diagram.Wherein, in illumination path, light source from
Projector projects, and the area source is wholly transmissive on spectroscope after the coding of projected image, is radiated at via spectroscope flat
On the reflection mirror array of face.In plane reflection lens array, each plane mirror can receive the light source of a part and will
The part light source reflexes to application scenarios where sample, and plane reflection lens array illuminates application scenarios where sample in total.At
As in optical path, sample reflection light is returned on each of plane reflection lens array plane mirror, each plane reflection
Mirror can receive sample image, and because the position angle of each plane mirror is different, so that each plane reflection
Image formed by mirror all has certain parallax；Plane reflection lens array is by the sample image at multiple visual angles via polarizing film transparent transmission
Afterwards, it reflexes on spectroscope, accessed by the CCD plane be just arranged by camera by spectroscope secondary reflection again, sample figure.
Fig. 5 is confocal passage conceptual schematic view provided in an embodiment of the present invention.The embodiment of the present invention is by changing plane
The angle of each of reflection mirror array plane mirror, to change application scenarios where illuminating bundle focuses on sample
Position.Illuminating bundle is radiated on sample focal plane 1 in such as figure, and is radiated on the focal plane 2 of sample, and illuminating bundle is formed
The radian and angle of plane reflection lens array are different.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent substitution, improvement and etc. done be should be included within the scope of the present invention.
Claims (12)
1. a kind of confocal imaging device characterized by comprising light source unit (11), polarizing film (13), is put down at spectroscope (12)
Face reflection mirror array (14) and imaging unit (15), wherein plane reflection lens array (14) is by multiple rotatable plane reflections
Mirror splices to obtain, and the plane mirror is seamless between any two and has setting in spliced plane reflection lens array (14)
Radian；
The white light mirror (12) that is split of light source unit (11) projection is transmitted on polarizing film (13)；Polarizing film (13) is to the projection light
The adjusting of polarization information is carried out, the white light after the adjusting forms illuminating bundle after the reflection of plane reflection lens array (14)
It focuses on the focusing surface of sample；
The focusing surface of sample reflects illuminating bundle, and the illuminating bundle of reflection successively passes through the anti-of plane reflection lens array (14)
It penetrates, after the reflection of the transparent transmission of polarizing film (13) and spectroscope (14), obtains sample focusing surface image by imaging unit (15).
2. device as described in claim 1, which is characterized in that the white light that the light source unit (11) is projected have passed through setting
Projected image coding after, then the mirror that is split (12) receive.
3. device as claimed in claim 2, which is characterized in that the polarizing film (13) carries out polarization information to the projection light
It is adjusted to the adjusting of horizontal polarization and vertical polarization；
The set projected image includes: the adjusting information of the horizontal polarization according to the polarizing film (13) and vertical polarization
The Depth profile information of the sample application scenarios is calculated, is arranged according to the Depth profile information of the sample application scenarios
Projected image；
The setting projected image changes according to the application scenarios of the sample, is adjusted or updates.
4. device as described in claim 1, which is characterized in that the light source unit (11) is projector；
The imaging unit (15) is charge coupled cell (CCD) plane of camera setting.
5. device as described in claim 1, which is characterized in that the angle of the plane reflection lens array (14) and the arc of setting
Degree is automatically adjusted.
6. device as described in claim 1, which is characterized in that the spectroscope (12) is that light transmission is more flat than the light splitting for 50:50
Piece, setting and the projection optical axis of described device are placed at 45 degree.
7. device as described in claim 1, which is characterized in that the position of the light source unit (11) and the imaging unit
(15) position is vertical, realizes coaxial imaging.
8. device as described in claim 1, which is characterized in that described device further includes computer, the imaging unit (15)
Acquisition sample focusing surface image is exported to be handled to the computer.
9. device as described in claim 1, which is characterized in that the application scenarios of described device are underwater.
10. a kind of confocal imaging method, which is characterized in that confocal imaging device as described in claim 1, this method packet is arranged
It includes:
The white light of light source unit projection in confocal imaging device is transmitted to confocal imaging by the spectroscope in confocal imaging device
On polarizing film in device；
After polarizing film in confocal imaging device carries out the adjusting of polarization information to the projection light, the white light after the adjusting is by altogether
After the reflection of plane reflection lens array in burnt imaging device, forms illuminating bundle and focus on the focusing surface of sample；
The focusing surface of sample reflects illuminating bundle, the illuminating bundle of reflection successively pass through plane reflection lens array reflection, partially
After the transparent transmission of vibration piece and spectroscopical reflection, sample focusing surface image is obtained by imaging unit.
11. method as claimed in claim 10, which is characterized in that the plane reflection lens array is by multiple rotatable planes
Reflecting mirror splices to obtain, and the plane mirror is seamless between any two and has setting in spliced plane reflection lens array
Radian.The angle and setting radian of the plane reflection lens array are controlled to adjust.
12. method as claimed in claim 10, which is characterized in that saturating by the spectroscope in confocal imaging device in the white light
Before being mapped on the polarizing film in confocal imaging device, further includes: the white light of the projection is carried out according to the projected image of setting
After coding, then received by the spectroscope；
The described pair of projection light carries out the adjusting for being adjusted to horizontal polarization and vertical polarization of polarization information；
The projected image of the setting includes: the adjusting letter according to described pair of projection light horizontal polarization carried out and vertical polarization
The Depth profile information of the sample application scenarios is calculated in breath, is set according to the Depth profile information of the sample application scenarios
Determine projected image；
The projected image of the setting changes according to the application scenarios of the sample, is adjusted or updates.
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| CN110826423A (en) * | 2019-10-18 | 2020-02-21 | 中北大学 | Method, device and system for detecting interested target in group target |
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