CN110184314A - A method of it co-cultures and improves microalgae grease yield - Google Patents
A method of it co-cultures and improves microalgae grease yield Download PDFInfo
- Publication number
- CN110184314A CN110184314A CN201910495132.9A CN201910495132A CN110184314A CN 110184314 A CN110184314 A CN 110184314A CN 201910495132 A CN201910495132 A CN 201910495132A CN 110184314 A CN110184314 A CN 110184314A
- Authority
- CN
- China
- Prior art keywords
- microalgae
- culture
- lipid
- algae
- plants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 59
- 239000004519 grease Substances 0.000 title claims abstract description 37
- 238000003501 co-culture Methods 0.000 title claims abstract description 32
- 241000195493 Cryptophyta Species 0.000 claims abstract description 62
- 150000002632 lipids Chemical class 0.000 claims abstract description 56
- 239000001963 growth medium Substances 0.000 claims abstract description 32
- 239000000843 powder Substances 0.000 claims abstract description 23
- 230000005526 G1 to G0 transition Effects 0.000 claims abstract description 21
- 238000004108 freeze drying Methods 0.000 claims abstract description 20
- 230000005791 algae growth Effects 0.000 claims abstract description 14
- 230000003698 anagen phase Effects 0.000 claims abstract description 12
- 238000005303 weighing Methods 0.000 claims abstract description 3
- 241000457035 Heveochlorella Species 0.000 claims description 38
- 241001478792 Monoraphidium Species 0.000 claims description 38
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 11
- 238000005286 illumination Methods 0.000 claims description 11
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- RDOXTESZEPMUJZ-UHFFFAOYSA-N anisole Chemical compound COC1=CC=CC=C1 RDOXTESZEPMUJZ-UHFFFAOYSA-N 0.000 claims description 4
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 4
- 239000000411 inducer Substances 0.000 claims description 3
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 2
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 2
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 229910052927 chalcanthite Inorganic materials 0.000 claims description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
- 229910052564 epsomite Inorganic materials 0.000 claims description 2
- 239000002509 fulvic acid Substances 0.000 claims description 2
- 229940095100 fulvic acid Drugs 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 238000012423 maintenance Methods 0.000 claims description 2
- 239000011565 manganese chloride Substances 0.000 claims description 2
- 239000012533 medium component Substances 0.000 claims description 2
- UZKWTJUDCOPSNM-UHFFFAOYSA-N methoxybenzene Substances CCCCOC=C UZKWTJUDCOPSNM-UHFFFAOYSA-N 0.000 claims description 2
- 239000003375 plant hormone Substances 0.000 claims description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 239000011684 sodium molybdate Substances 0.000 claims description 2
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- 239000011686 zinc sulphate Substances 0.000 claims description 2
- 238000012258 culturing Methods 0.000 abstract description 6
- 239000003225 biodiesel Substances 0.000 abstract description 4
- 230000003993 interaction Effects 0.000 abstract description 3
- 238000005119 centrifugation Methods 0.000 abstract description 2
- 125000005456 glyceride group Chemical group 0.000 abstract 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 36
- 239000002028 Biomass Substances 0.000 description 20
- 239000006228 supernatant Substances 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 12
- 238000005057 refrigeration Methods 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 6
- 244000046052 Phaseolus vulgaris Species 0.000 description 6
- 235000012149 noodles Nutrition 0.000 description 6
- 238000010899 nucleation Methods 0.000 description 6
- 230000012010 growth Effects 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000002803 fossil fuel Substances 0.000 description 3
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000036579 abiotic stress Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229960002413 ferric citrate Drugs 0.000 description 1
- -1 ferric citrate amine Chemical class 0.000 description 1
- 238000003209 gene knockout Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000013370 mutualism Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of methods for co-culturing and improving microalgae grease yield, the following steps are included: (1) seed liquor culture: cultivating two plants of microalgaes respectively with BG-11 culture medium, frustule is collected to micro algae growth to logarithmic growth phase later period, as seed liquor；(2) it co-cultures: accessing two plants of microalgae seed liquors of step (1) culture simultaneously in BG-11 culture medium, co-cultured；(3) it co-cultures the collection of microalgae: the co-cultivation algae solution of culture to stationary phase is obtained into algae powder by the method for centrifugation and freeze-drying；Then by after gained algae powder weighing in step (3), fat content is measured with Bligh&Dyer method, then obtain lipid-producing by calculating.The present invention can largely accumulate grease by interaction of microalgae under the conditions of co-cultivation in a short time, improve microalgae grease yield, be conducive to provide glyceride stock for the large-scale production of biodiesel.
Description
Technical field
The invention belongs to technical field of energy microalgae, and in particular to a method of it co-cultures and improves microalgae grease yield.
Background technique
Due to the increasingly failure of conventional fossil fuel and the growing tension of environmental problem, to the renewable environmentally friendly energy
Exploration demand it is also more more and more urgent.It is a kind of no pollution to the environment as the third generation biodiesel of raw material using microalgae grease and can
The regenerated new bio energy, technically can be produced, but due to higher cost, not yet realization large-scale industry
Change.The key of microalgae oil-producing is raising lipid-producing, and lower lipid-producing is microalgae oil-producing relative to conventional fossil fuel
Disadvantage, also fail to be well solved at present.Therefore, lipid-producing is improved, is to realize heavy industrialization microalgae oil-producing
The task of top priority.
It is existing improve lipid-producing method include gene knockout, addition inducer, abiotic stress, addition metal from
Son, fermentation tank culture and raceway pond culture etc..These methods there is low efficiency, need to increase additional equipment, early investment compared with
Greatly, the defects of at high cost.
Compared to the above, the co-cultivation strategy of microalgae can effectively avoid above method problems faced.There is research table
Bright, co-cultivation can make to generate competitive relation between two kinds of microalgaes in cultivating system, promote the synthesis of grease, and then improve grease and contain
Amount and lipid-producing, and have many advantages, such as that low energy consumption, high-efficient, at low cost and pollution is small.
In conclusion a kind of method for finding low cost raising microalgae grease yield is very necessary, raising microalgae oil
Rouge yield is the key point for changing biodiesel relative to conventional fossil fuel.
Summary of the invention
With deepening continuously to microbe research, many researcher's discoveries, many important biochemical processes are single micro-
It is difficult to or faint can only carry out under biological culture, need just to be able to achieve by two or more microorganism co-incubations.It co-cultures
Refer to two or more microorganism syntrophism, the microorganism under co-culturing under same environment, same condition of culture
It is known, specific.Microbes under the conditions of co-cultivation generate complicated interaction, such as vie each other, mutually inhibit
Or mutualism etc., this accumulation for certain metabolites such as grease can play a positive role.
The purpose of the present invention is to provide a kind of method for co-culturing and improving microalgae grease yield, microalgae oil-producing is at low cost
Honest and clean, safety and environmental protection and easy to operate.
The technical scheme adopted by the invention is that:
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) seed liquor culture: two plants of microalgaes are cultivated respectively with BG-11 culture medium, after micro algae growth to logarithmic growth phase
Phase collects frustule, as seed liquor；
(2) it co-cultures: accessing two plants of microalgae seed liquors of step (1) culture simultaneously in BG-11 culture medium, carry out total training
It supports；
(3) collection of microalgae is co-cultured: the side by the co-cultivation algae solution of culture to stationary phase by centrifugation and freeze-drying
Method obtains dry algae powder.
Further, two plants of microalgae types are single needle algae Monoraphidium sp. (FACHB-1821) and gum
Chlorella Heveochlorella sp. (MK829186).
Further, the condition of culture in step (1) and step (2) are as follows: 2500~4000lux of intensity of illumination, shaking table turn
Speed is 150r/min, and cultivation temperature is 25 ± 1 DEG C, and maintenance system pH is 6.5~7.5.
Further, the BG-11 medium component is NaNO31.5g/L, K2HPO40.04g/L, MgSO4·7H2O
0.075g/L, CaCl2·2H2O 0.036g/L, citric acid 0.006g/L, ferric citrate amine 0.006g/L, EDTANa20.006g/
L, Na2CO30.02g/L, A5 microelement 1.0mL/L, inducer (10 μ of plant hormone fulvic acid FA25mg/L, epiphysin MT
Mol/L, anisole BHA2mg/L).
Further, the A5 microelement is by H3BO32.86g/L MnCl2·4H2O 1.81g/L, ZnSO4·7H2O
0.222g/L, Na2MoO4·2H2O 0.39g/L, CuSO4·5H2O 0.079g/L, Co (NO3)2·6H2O 0.0494g/L configuration
It forms.
Further, glucose C is added6H12O610g/L is as carbon source.
Further, the initial cell gross density of two plants of microalgaes is 1.00 × 10 in co-culture system in step (2)6~
9.00×106Cells/mL, the density content of two plants of microalgaes are 1:3~3:1.
Further, the method for freeze-drying described in step (3) is that algae solution is placed in -80 DEG C of refrigerator (Thermo
Scientific 900Series) freeze overnight, it is subsequently placed in vacuum freeze drier (SIM FD5-12) and is freeze-dried 48h
Obtain dry algae powder.
Further, by after gained algae powder weighing in step (3), fat content is measured with Bligh&Dyer method, then pass through
It calculates and obtains lipid-producing.
Compared with prior art, the invention has the advantages that and technical effect:
1, the bottleneck that present invention is low for microalgae oil-producing lipid-producing problem provides a kind of co-cultivation raising microalgae oil
The method of rouge yield, co-cultivation condition make two kinds of microalgaes produce competitive relation, and induction each produces independent culture
Under the conditions of the biochemical reaction that not will do it, the system for producing different from the metabolite under independent condition of culture, and co-culturing
Middle Study on nitrate concentration runs out of earlier, and the wear rate of nitrogen source is promoted in the process, faster enters nitrogen restriction state,
To carry out oil and fat accumulation.The main co-cultivation by between two plants of microalgaes causes the interaction between microalgae, accelerates consumption
Nitrogen source in environment can preferably realize the limitation of nitrogen source in culture quickly.
2, compared to the method for largely adding various chemical reagent raising microalgae grease yields, the present invention is at low cost, chemistry
Substance dosage is few, and clean and safe, will not generate secondary pollution.
3, the technology of the present invention is easy to operate, low in cost, clean and effective, and there is microalgae grease yield to improve significant effect
Fruit, the significant effect in terms of the application and development of microalgae, has a extensive future.
4, single needle algae Monoraphidium sp. (FACHB-1821) and gum chlorella Heveochlorella sp.
(MK829186) nutrient growth mode is close, and specific growth rate is higher, is appropriate for co-culturing；And fat content is higher, rouge
Fat acid composition reaches the relevant criterion of production biodiesel, and two plants of microalgaes can preferably be grown under same culture conditions.
5, during co-culturing, microalgae inoculation algae density is extremely important to average biomass and lipid-producing, inoculum density
Too low, micro algae growth lag phase side length, average biomass and lipid-producing decline；Inoculum density is excessively high, same in seeded process
When be introduced into discarded culture medium and metabolic waste in seed liquor, although micro algae growth lag phase shorten, discard culture medium and
Metabolic waste hinders the growth of microalgae, and average biomass and lipid-producing can also decline, therefore the invention discloses suitable
The density content of microalgae initial cell gross density and two plants of microalgaes, two plants of algaes can preferably grow and can cooperate with oil-producing, while
Growth cycle can be shortened and prevent living contaminants, higher biomass and lipid-producing can be obtained on this basis.
6, the present invention co-cultures single needle algae Monoraphidium sp. (FACHB-1821) and gum chlorella
Heveochlorella sp. (MK829186) is relative to independent culture single needle algae Monoraphidium sp. (FACHB-
1821), gum chlorella Heveochlorella sp. (MK829186), average biomass increase 1.07~1.44 times,
Lipid-producing increases 1.15~2.03 times.
Specific embodiment
The present invention is described in further details combined with specific embodiments below.
Comparative example 1
For the superiority for absolutely proving the method for the present invention, comparative example 1 is single needle algae Monoraphidium sp. (FACHB-
1821) lipid-producing after individually cultivating, key step are as follows: be in 25 ± 1 DEG C, light intensity 3500lux, shaking speed
Under conditions of 150r/min, according to 4.00 × 106The Initial seeding density of cells/mL, using BG-11 as culture media shaking vase culture
Single needle algae Monoraphidium sp. (FACHB-1821) 9 days is to stationary phase.It will be cultivated with centrifuge (TDL-40B) to stabilization
The algae solution cell of phase is centrifuged 10min at 3500rpm, and removal supernatant obtains wet frond, in -80 DEG C of refrigerator (Thermo
Scientific 900Series) freeze overnight, algae must be done by being placed in freeze-drying 48h in vacuum freeze drier (FD5-12)
Powder.
The measuring method of lipid-producing: a ten thousandth balance (Sartorius BSA124S) weighs gained xeraphium quality
W measures microalgae grease content C using Bligh&Dyer methodLipid(%), then the lipid-producing P of microalgaeLipidAre as follows:
PLipid(mg·L-1·d-1)=W(mg)×CLipid(%)/V(L)×T(d)
In formula, PLipid--- lipid-producing (mgL-1·d-1)；W --- algae dried bean noodles weight (mg)；CLipid--- grease contains
It measures (%)；V --- working volume (L)；T --- cultivated days (d).
As a result: the control group average biomass is 3.8g/L, and average lipid-producing is 170.8mgL-1·d-1。
Comparative example 2
For the superiority for absolutely proving the method for the present invention, comparative example 2 is gum chlorella Heveochlorella sp.
(MK829186) lipid-producing after individually cultivating, key step are as follows: turn in 25 ± 1 DEG C, light intensity 3500lux, shaking table
Under conditions of speed is 150r/min, according to 4.00 × 106The Initial seeding density of cells/mL, using BG-11 as culture media shaking vase
Gum chlorella Heveochlorella sp. (MK829186) 9 days is cultivated to stationary phase.It will be cultivated with centrifuge (TDL-40B)
Algae solution cell to stationary phase is centrifuged 10min at 3500rpm, and removal supernatant obtains wet frond, in -80 DEG C of refrigerator (Thermo
Scientific 900Series) freeze overnight, being placed in freeze-drying 48h in vacuum freeze drier (SIM FD5-12) must do
Algae powder.
The measuring method of lipid-producing: a ten thousandth balance (Sartorius BSA124S) weighs gained xeraphium quality
W measures microalgae grease content C using Bligh&Dyer methodLipid(%), then the lipid-producing P of microalgaeLipidAre as follows:
PLipid(mg·L-1·d-1)=W(mg)×CLipid(%)/V(L)×T(d)
In formula, PLipid--- lipid-producing (mgL-1·d-1)；W --- algae dried bean noodles weight (mg)；CLipid--- grease contains
It measures (%)；V --- working volume (L)；T --- cultivated days (d).
As a result: the control group average biomass is 1.2g/L, and average lipid-producing is 51mgL-1·d-1。
Embodiment 1
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) at 25 ± 1 DEG C, light intensity 3500lux, under conditions of shaking speed is 150r/min, using BG-11 as culture medium point
Not Pei Yang two plants of microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186), to
Micro algae growth to the logarithmic growth phase later period collects frustule, the seed liquor as co-cultivation；
(2) seed liquor of two plants of microalgaes, is co-cultured more than accessing simultaneously in BG-11 culture medium.Intensity of illumination
3500lux, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C.The cell gross density of two plants of microalgaes in co-culture system
It is 4.00 × 106Cells/mL, microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp.
(MK829186) initial cell density is all 2.00 × 106Cells/mL, at this time microalgae Monoraphidium sp.
(FACHB-1821) and Heveochlorella sp. (MK829186) initial cell ratio is 1:1；
(3) it will be cultivated to the algae solution cell of stationary phase with centrifuge (TDL-40B) and be centrifuged 10min at 3500rpm, removed
Supernatant obtains wet frond, and in -80 DEG C of refrigerators (Thermo Scientific 900Series) freeze overnight, it is dry to be placed in vacuum refrigeration
Freeze-drying 48h obtains dry algae powder in dry machine (SIM FD5-12), measures lipid-producing.
As a result: the control group average biomass is 3.3g/L, and average lipid-producing is 203.8mgL-1·d-1。
Embodiment 2
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) at 25 ± 1 DEG C, light intensity 3500lux, under conditions of shaking speed is 150r/min, using BG-11 as culture medium point
Not Pei Yang two plants of microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186), to
Micro algae growth to the logarithmic growth phase later period collects frustule, the seed liquor as co-cultivation；
(2) seed liquor of two plants of microalgaes, is co-cultured more than accessing simultaneously in BG-11 culture medium.Intensity of illumination
3500lux, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C.The cell gross density of two plants of microalgaes in co-culture system
It is 4.00 × 106The initial cell density of cells/mL, microalgae Monoraphidium sp. (FACHB-1821) be 1.00 ×
106The initial cell density of cells/mL, Heveochlorella sp. (MK829186) are all 3.00 × 106Cells/mL, this
Shi Weizao Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186) initial cell ratio are
1:3；
(3) it will be cultivated to the algae solution cell of stationary phase with centrifuge (TDL-40B) and be centrifuged 10min at 3500rpm, removed
Supernatant obtains wet frond, and in -80 DEG C of refrigerators (Thermo Scientific 900Series) freeze overnight, it is dry to be placed in vacuum refrigeration
Freeze-drying 48h obtains dry algae powder in dry machine (SIM FD5-12), measures lipid-producing.
As a result: the control group average biomass is 2.7g/L, and average lipid-producing is 120.7mgL-1·d-1。
Embodiment 3
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) at 25 ± 1 DEG C, light intensity 3500lux, under conditions of shaking speed is 150r/min, using BG-11 as culture medium point
Not Pei Yang two plants of microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186), to
Micro algae growth to the logarithmic growth phase later period collects frustule, the seed liquor as co-cultivation；
(2) seed liquor of two plants of microalgaes, is co-cultured more than accessing simultaneously in BG-11 culture medium.Intensity of illumination
3500lux, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C.The cell gross density of two plants of microalgaes in co-culture system
It is 4.00 × 106The initial cell density of cells/mL, microalgae Monoraphidium sp. (FACHB-1821) be 3.00 ×
106The initial cell density of cells/mL, Heveochlorella sp. (MK829186) are all 1.00 × 106Cells/mL, this
Shi Weizao Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186) initial cell ratio are
3:1；
(3) it will be cultivated to the algae solution cell of stationary phase with centrifuge (TDL-40B) and be centrifuged 10min at 3500rpm, removed
Supernatant obtains wet frond, and in -80 DEG C of refrigerators (Thermo Scientific 900Series) freeze overnight, it is dry to be placed in vacuum refrigeration
Freeze-drying 48h obtains dry algae powder in dry machine (SIM FD5-12), measures lipid-producing.
As a result: the control group average biomass is 3.5g/L, and average lipid-producing is 162.2mgL-1·d-1。
Comparative example 3
For the superiority for absolutely proving the method for the present invention, comparative example 1 is single needle algae Monoraphidium sp. (FACHB-
1821) lipid-producing after individually cultivating, key step are as follows: be in 25 ± 1 DEG C, light intensity 2500lux, shaking speed
Under conditions of 150r/min, according to 1.00 × 106The Initial seeding density of cells/mL, using BG-11 as culture media shaking vase culture
Single needle algae Monoraphidium sp. (FACHB-1821) 9 days is to stationary phase.It will be cultivated with centrifuge (TDL-40B) to stabilization
The algae solution cell of phase is centrifuged 10min at 3500rpm, and removal supernatant obtains wet frond, in -80 DEG C of refrigerator (Thermo
Scientific 900Series) freeze overnight, algae must be done by being placed in freeze-drying 48h in vacuum freeze drier (FD5-12)
Powder.
The measuring method of lipid-producing: a ten thousandth balance (Sartorius BSA124S) weighs gained xeraphium quality
W measures microalgae grease content C using Bligh&Dyer methodLipid(%), then the lipid-producing P of microalgaeLipidAre as follows:
PLipid(mg·L-1·d-1)=W(mg)×CLipid(%)/V(L)×T(d)
In formula, PLipid--- lipid-producing (mgL-1·d-1)；W --- algae dried bean noodles weight (mg)；CLipid--- grease contains
It measures (%)；V --- working volume (L)；T --- cultivated days (d).
As a result: the control group average biomass is 3.1g/L, and average lipid-producing is 140.4mgL-1·d-1。
Comparative example 4
For the superiority for absolutely proving the method for the present invention, comparative example 2 is gum chlorella Heveochlorella sp.
(MK829186) lipid-producing after individually cultivating, key step are as follows: turn in 25 ± 1 DEG C, light intensity 2500lux, shaking table
Under conditions of speed is 150r/min, according to 1.00 × 106The Initial seeding density of cells/mL, using BG-11 as culture media shaking vase
Gum chlorella Heveochlorella sp. (MK829186) 9 days is cultivated to stationary phase.It will be cultivated with centrifuge (TDL-40B)
Algae solution cell to stationary phase is centrifuged 10min at 3500rpm, and removal supernatant obtains wet frond, in -80 DEG C of refrigerator (Thermo
Scientific 900Series) freeze overnight, being placed in freeze-drying 48h in vacuum freeze drier (SIM FD5-12) must do
Algae powder.
The measuring method of lipid-producing: a ten thousandth balance (Sartorius BSA124S) weighs gained xeraphium quality
W measures microalgae grease content C using Bligh&Dyer methodLipid(%), then the lipid-producing P of microalgaeLipidAre as follows:
PLipid(mg·L-1·d-1)=W(mg)×CLipid(%)/V(L)×T(d)
In formula, PLipid--- lipid-producing (mgL-1·d-1)；W --- algae dried bean noodles weight (mg)；CLipid--- grease contains
It measures (%)；V --- working volume (L)；T --- cultivated days (d).
As a result: the control group average biomass is 0.7g/L, and average lipid-producing is 29.7mgL-1·d-1。
Embodiment 4
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) at 25 ± 1 DEG C, light intensity 2500lux, under conditions of shaking speed is 150r/min, using BG-11 as culture medium point
Not Pei Yang two plants of microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186), to
Micro algae growth to the logarithmic growth phase later period collects frustule, the seed liquor as co-cultivation；
(2) seed liquor of two plants of microalgaes, is co-cultured more than accessing simultaneously in BG-11 culture medium.Intensity of illumination
2500lux, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C.The cell gross density of two plants of microalgaes in co-culture system
It is 1.00 × 106Cells/mL, microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp.
(MK829186) initial cell density is all 5.00 × 105Cells/mL, at this time microalgae Monoraphidium sp.
(FACHB-1821) and Heveochlorella sp. (MK829186) initial cell ratio is 1:1；
(3) it will be cultivated to the algae solution cell of stationary phase with centrifuge (TDL-40B) and be centrifuged 10min at 3500rpm, removed
Supernatant obtains wet frond, and in -80 DEG C of refrigerators (Thermo Scientific 900Series) freeze overnight, it is dry to be placed in vacuum refrigeration
Freeze-drying 48h obtains dry algae powder in dry machine (SIM FD5-12), measures lipid-producing.
As a result: the control group average biomass is 2.8g/L, and average lipid-producing is 172.8mgL-1·d-1。
Embodiment 5
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) at 25 ± 1 DEG C, light intensity 2500lux, under conditions of shaking speed is 150r/min, using BG-11 as culture medium point
Not Pei Yang two plants of microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186), to
Micro algae growth to the logarithmic growth phase later period collects frustule, the seed liquor as co-cultivation；
(2) seed liquor of two plants of microalgaes, is co-cultured more than accessing simultaneously in BG-11 culture medium.Intensity of illumination
2500lux, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C.The cell gross density of two plants of microalgaes in co-culture system
It is 1.00 × 106The initial cell density of cells/mL, microalgae Monoraphidium sp. (FACHB-1821) be 2.50 ×
105The initial cell density of cells/mL, Heveochlorella sp. (MK829186) are all 7.50 × 105Cells/mL, this
Shi Weizao Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186) initial cell ratio are
1:3；
(3) it will be cultivated to the algae solution cell of stationary phase with centrifuge (TDL-40B) and be centrifuged 10min at 3500rpm, removed
Supernatant obtains wet frond, and in -80 DEG C of refrigerators (Thermo Scientific 900Series) freeze overnight, it is dry to be placed in vacuum refrigeration
Freeze-drying 48h obtains dry algae powder in dry machine (SIM FD5-12), measures lipid-producing.
As a result: the control group average biomass is 2.1g/L, and average lipid-producing is 93.9mgL-1·d-1。
Embodiment 6
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) at 25 ± 1 DEG C, light intensity 2500lux, under conditions of shaking speed is 150r/min, using BG-11 as culture medium point
Not Pei Yang two plants of microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186), to
Micro algae growth to the logarithmic growth phase later period collects frustule, the seed liquor as co-cultivation；
(2) seed liquor of two plants of microalgaes, is co-cultured more than accessing simultaneously in BG-11 culture medium.Intensity of illumination
2500lux, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C.The cell gross density of two plants of microalgaes in co-culture system
It is 1.00 × 106The initial cell density of cells/mL, microalgae Monoraphidium sp. (FACHB-1821) be 7.50 ×
105The initial cell density of cells/mL, Heveochlorella sp. (MK829186) are all 2.50 × 105Cells/mL, this
Shi Weizao Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186) initial cell ratio are
3:1；
(3) it will be cultivated to the algae solution cell of stationary phase with centrifuge (TDL-40B) and be centrifuged 10min at 3500rpm, removed
Supernatant obtains wet frond, and in -80 DEG C of refrigerators (Thermo Scientific 900Series) freeze overnight, it is dry to be placed in vacuum refrigeration
Freeze-drying 48h obtains dry algae powder in dry machine (SIM FD5-12), measures lipid-producing.
As a result: the control group average biomass is 3.0g/L, and average lipid-producing is 138.4mgL-1·d-1。
Comparative example 5
For the superiority for absolutely proving the method for the present invention, comparative example 1 is single needle algae Monoraphidium sp. (FACHB-
1821) lipid-producing after individually cultivating, key step are as follows: be in 25 ± 1 DEG C, light intensity 4000lux, shaking speed
Under conditions of 150r/min, according to 9.00 × 106The Initial seeding density of cells/mL, using BG-11 as culture media shaking vase culture
Single needle algae Monoraphidium sp. (FACHB-1821) 9 days is to stationary phase.It will be cultivated with centrifuge (TDL-40B) to stabilization
The algae solution cell of phase is centrifuged 10min at 3500rpm, and removal supernatant obtains wet frond, in -80 DEG C of refrigerator (Thermo
Scientific 900Series) freeze overnight, algae must be done by being placed in freeze-drying 48h in vacuum freeze drier (FD5-12)
Powder.
The measuring method of lipid-producing: a ten thousandth balance (Sartorius BSA124S) weighs gained xeraphium quality
W measures microalgae grease content C using Bligh&Dyer methodLipid(%), then the lipid-producing P of microalgaeLipidAre as follows:
PLipid(mg·L-1·d-1)=W(mg)×CLipid(%)/V(L)×T(d)
In formula, PLipid--- lipid-producing (mgL-1·d-1)；W --- algae dried bean noodles weight (mg)；CLipid--- grease contains
It measures (%)；V --- working volume (L)；T --- cultivated days (d).
As a result: the control group average biomass is 3.4g/L, and average lipid-producing is 150.2mgL-1·d-1。
Comparative example 6
For the superiority for absolutely proving the method for the present invention, comparative example 2 is gum chlorella Heveochlorella sp.
(MK829186) lipid-producing after individually cultivating, key step are as follows: turn in 25 ± 1 DEG C, light intensity 4000lux, shaking table
Under conditions of speed is 150r/min, according to 9.00 × 106The Initial seeding density of cells/mL, using BG-11 as culture media shaking vase
Gum chlorella Heveochlorella sp. (MK829186) 9 days is cultivated to stationary phase.It will be cultivated with centrifuge (TDL-40B)
Algae solution cell to stationary phase is centrifuged 10min at 3500rpm, and removal supernatant obtains wet frond, in -80 DEG C of refrigerator (Thermo
Scientific 900Series) freeze overnight, being placed in freeze-drying 48h in vacuum freeze drier (SIM FD5-12) must do
Algae powder.
The measuring method of lipid-producing: a ten thousandth balance (Sartorius BSA124S) weighs gained xeraphium quality
W measures microalgae grease content C using Bligh&Dyer methodLipid(%), then the lipid-producing P of microalgaeLipidAre as follows:
PLipid(mg·L-1·d-1)=W(mg)×CLipid(%)/V(L)×T(d)
In formula, PLipid--- lipid-producing (mgL-1·d-1)；W --- algae dried bean noodles weight (mg)；CLipid--- grease contains
It measures (%)；V --- working volume (L)；T --- cultivated days (d).
As a result: the control group average biomass is 1.0g/L, and average lipid-producing is 40mgL-1·d-1。
Embodiment 7
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) at 25 ± 1 DEG C, light intensity 4000lux, under conditions of shaking speed is 150r/min, using BG-11 as culture medium point
Not Pei Yang two plants of microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186), to
Micro algae growth to the logarithmic growth phase later period collects frustule, the seed liquor as co-cultivation；
(2) seed liquor of two plants of microalgaes, is co-cultured more than accessing simultaneously in BG-11 culture medium.Intensity of illumination
4000lux, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C.The cell gross density of two plants of microalgaes in co-culture system
It is 9.00 × 106Cells/mL, microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp.
(MK829186) initial cell density is all 4.50 × 106Cells/mL, at this time microalgae Monoraphidium sp.
(FACHB-1821) and Heveochlorella sp. (MK829186) initial cell ratio is 1:1；
(3) it will be cultivated to the algae solution cell of stationary phase with centrifuge (TDL-40B) and be centrifuged 10min at 3500rpm, removed
Supernatant obtains wet frond, and in -80 DEG C of refrigerators (Thermo Scientific 900Series) freeze overnight, it is dry to be placed in vacuum refrigeration
Freeze-drying 48h obtains dry algae powder in dry machine (SIM FD5-12), measures lipid-producing.
As a result: the control group average biomass is 3.0g/L, and average lipid-producing is 180.4mgL-1·d-1。
Embodiment 8
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) at 25 ± 1 DEG C, light intensity 4000lux, under conditions of shaking speed is 150r/min, using BG-11 as culture medium point
Not Pei Yang two plants of microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186), to
Micro algae growth to the logarithmic growth phase later period collects frustule, the seed liquor as co-cultivation；
(2) seed liquor of two plants of microalgaes, is co-cultured more than accessing simultaneously in BG-11 culture medium.Intensity of illumination
4000lux, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C.The cell gross density of two plants of microalgaes in co-culture system
It is 9.00 × 106The initial cell density of cells/mL, microalgae Monoraphidium sp. (FACHB-1821) be 2.25 ×
106The initial cell density of cells/mL, Heveochlorella sp. (MK829186) are all 6.75 × 106Cells/mL, this
Shi Weizao Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186) initial cell ratio are
1:3；
(3) it will be cultivated to the algae solution cell of stationary phase with centrifuge (TDL-40B) and be centrifuged 10min at 3500rpm, removed
Supernatant obtains wet frond, and in -80 DEG C of refrigerators (Thermo Scientific 900Series) freeze overnight, it is dry to be placed in vacuum refrigeration
Freeze-drying 48h obtains dry algae powder in dry machine (SIM FD5-12), measures lipid-producing.
As a result: the control group average biomass is 2.3g/L, and average lipid-producing is 105.2mgL-1·d-1。
Embodiment 9
A method of it co-cultures and improves microalgae grease yield, comprising the following steps:
(1) at 25 ± 1 DEG C, light intensity 4000lux, under conditions of shaking speed is 150r/min, using BG-11 as culture medium point
Not Pei Yang two plants of microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186), to
Micro algae growth to the logarithmic growth phase later period collects frustule, the seed liquor as co-cultivation；
(2) seed liquor of two plants of microalgaes, is co-cultured more than accessing simultaneously in BG-11 culture medium.Intensity of illumination
4000lux, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C.The cell gross density of two plants of microalgaes in co-culture system
It is 9.00 × 106The initial cell density of cells/mL, microalgae Monoraphidium sp. (FACHB-1821) be 6.75 ×
106The initial cell density of cells/mL, Heveochlorella sp. (MK829186) are 2.25 × 106Cells/mL, at this time
Microalgae Monoraphidium sp. (FACHB-1821) and Heveochlorella sp. (MK829186) initial cell ratio are 3:
1；
(3) it will be cultivated to the algae solution cell of stationary phase with centrifuge (TDL-40B) and be centrifuged 10min at 3500rpm, removed
Supernatant obtains wet frond, and in -80 DEG C of refrigerators (Thermo Scientific 900Series) freeze overnight, it is dry to be placed in vacuum refrigeration
Freeze-drying 48h obtains dry algae powder in dry machine (SIM FD5-12), measures lipid-producing.
As a result: the control group average biomass is 3.1g/L, and average lipid-producing is 145.2mgL-1·d-1。
The above is only preferable case of the invention, does not make any restrictions to the present invention, all for the present invention
Any simple modification, alteration or imitation that technology contents do the above case study on implementation belongs to the protection of technical solution of the present invention
Range.
Claims (9)
1. a kind of co-culture the method for improving microalgae grease yield, which comprises the following steps:
(1) seed liquor culture: cultivating two plants of microalgaes with BG-11 culture medium respectively, receives to micro algae growth to logarithmic growth phase later period
Collect frustule, as seed liquor；
(2) it co-cultures: accessing two plants of microalgae seed liquors of step (1) culture simultaneously in BG-11 culture medium, co-cultured；
(3) it co-cultures the collection of microalgae: the co-cultivation algae solution of culture to stationary phase is obtained by the method for being centrifuged and being freeze-dried
Obtain dry algae powder.
2. the method according to claim 1, wherein two plants of microalgae types are single needle algae
Monoraphidium sp. (FACHB-1821) and gum chlorella Heveochlorella sp. (MK829186).
3. the method according to claim 1, wherein the condition of culture in step (1) and step (2) are as follows: illumination
2500~4000lux of intensity, shaking speed 150r/min, cultivation temperature are 25 ± 1 DEG C, and maintenance system pH is 6.5~7.5.
4. the method according to claim 1, wherein the BG-11 medium component is NaNO31.5g/L
K2HPO40.04g/L, MgSO4·7H2O 0.075g/L, CaCl2·2H2O 0.036g/L, citric acid 0.006g/L, citric acid
Sideramines 0.006g/L, EDTANa20.006g/L, Na2CO30.02g/L, A5 microelement 1.0mL/L, inducer (plant hormone
Fulvic acid FA25mg/L, epiphysin MT 10 μm of ol/L, anisole BHA2mg/L).
5. according to the method described in claim 4, it is characterized in that, the A5 microelement is by H3BO32.86g/L MnCl2·
4H2O 1.81g/L, ZnSO4·7H2O 0.222g/L, Na2MoO4·2H2O 0.39g/L, CuSO4·5H2O 0.079g/L, Co
(NO3)2·6H2O 0.0494g/L is configured.
6. according to the method described in claim 5, it is characterized in that, glucose C is added6H12O610g/L is as carbon source.
7. the method according to claim 1, wherein in step (2) in co-culture system two plants of microalgaes it is initial
Cell gross density is 1.00 × 106~4.00 × 106Cells/mL, the density content of two plants of microalgaes are 1:3~3:1.
8. the method according to claim 1, wherein the method for freeze-drying described in step (3) is by algae solution
It is placed in -80 DEG C of refrigerators (900 Series of Thermo Scientific) freeze overnight, is subsequently placed in vacuum freeze drier
Freeze-drying 48h obtains dry algae powder in (SIM FD5-12).
9. the method according to claim 1, wherein using Bligh& for after gained algae powder weighing in step (3)
Dyer method measures fat content, then obtains lipid-producing by calculating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910495132.9A CN110184314A (en) | 2019-06-10 | 2019-06-10 | A method of it co-cultures and improves microalgae grease yield |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910495132.9A CN110184314A (en) | 2019-06-10 | 2019-06-10 | A method of it co-cultures and improves microalgae grease yield |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110184314A true CN110184314A (en) | 2019-08-30 |
Family
ID=67720791
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910495132.9A Pending CN110184314A (en) | 2019-06-10 | 2019-06-10 | A method of it co-cultures and improves microalgae grease yield |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110184314A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587916A (en) * | 2018-05-23 | 2018-09-28 | 昆明理工大学 | A method of co-culturing single needle algae rapid flocculation in neutral conditions |
| CN114015493A (en) * | 2021-09-26 | 2022-02-08 | 上海师范大学 | Method for extracting grease from algae |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587916A (en) * | 2018-05-23 | 2018-09-28 | 昆明理工大学 | A method of co-culturing single needle algae rapid flocculation in neutral conditions |
-
2019
- 2019-06-10 CN CN201910495132.9A patent/CN110184314A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587916A (en) * | 2018-05-23 | 2018-09-28 | 昆明理工大学 | A method of co-culturing single needle algae rapid flocculation in neutral conditions |
Non-Patent Citations (4)
| Title |
|---|
| WILLIAM B. SANDERS等: "HEVEOCHLORELLA (TREBOUXIOPHYCEAE): A LITTLE-KNOWN GENUS OF UNICELLULAR GREEN ALGAE OUTSIDE THE TREBOUXIALES EMERGES UNEXPECTEDLY AS A MAJOR CLADE OF LICHEN PHOTOBIONTS IN FOLIICOLOUS COMMUNITIES", 《J. PHYCOL.》 * |
| 左正三 等: "微藻生产油脂培养新技术", 《中国生物工程杂志》 * |
| 李大菲: "光化学诱导异养培养单针藻产油脂的研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
| 赵鹏: "产油微藻的分离及藻—藻共培养的研究", 《中国博士学位论文全文数据库 基础科学辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587916A (en) * | 2018-05-23 | 2018-09-28 | 昆明理工大学 | A method of co-culturing single needle algae rapid flocculation in neutral conditions |
| CN114015493A (en) * | 2021-09-26 | 2022-02-08 | 上海师范大学 | Method for extracting grease from algae |
| CN114015493B (en) * | 2021-09-26 | 2024-02-20 | 上海师范大学 | Method for extracting grease from algae |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhu et al. | Recycling harvest water to cultivate Chlorella zofingiensis under nutrient limitation for biodiesel production | |
| Chu et al. | Phosphorus plays an important role in enhancing biodiesel productivity of Chlorella vulgaris under nitrogen deficiency | |
| Feng et al. | Lipid production of Chlorella vulgaris cultured in artificial wastewater medium | |
| CN109609382B (en) | Method for promoting growth of chlorella and oil accumulation by algal-bacteria co-culture | |
| Ji et al. | Biofilm cultivation of the oleaginous microalgae Pseudochlorococcum sp. | |
| CN106467896B (en) | A kind of kelvin being resistant to high PH intends chlorella and its culture application | |
| CN106754383B (en) | Method for improving microbial biomass and oil yield | |
| Ji et al. | An applicable nitrogen supply strategy for attached cultivation of Aucutodesmus obliquus | |
| Ramaraj et al. | Microalgae biomass as an alternative substrate in biogas production | |
| CN110684667B (en) | Microalgae biofilm culture method capable of simultaneously improving biomass and grease yield | |
| CN104611228A (en) | Highly oil-containing monoraphidium and culture and application thereof | |
| Carlozzi et al. | Production of bio-fuels (hydrogen and lipids) through a photofermentation process | |
| CN105648023B (en) | A kind of method that microalgae mixed culture prepares grease | |
| CN102268377A (en) | Method for improving lipid producing microalga biomass and lipid accumulation with two stage culture strategy of mixotrophic and nitrogen-rich-nitrogen-deficient culture | |
| CN105671095A (en) | Cultivation method for promoting rapid accumulation of oil producing microalgae cells and grease | |
| CN104152357A (en) | High-density culture method for improving chlorophyll and protein content of chlorella at same time | |
| CN102220241B (en) | Cultivated fresh water microalgae and application thereof in production of biodiesel and nervonic acid | |
| Zhao et al. | Effects of trophic modes, carbon sources, and salinity on the cell growth and lipid accumulation of tropic ocean oilgae strain Desmodesmus sp. WC08 | |
| CN110184314A (en) | A method of it co-cultures and improves microalgae grease yield | |
| CN107118968B (en) | Chlorella sorokiniana synthesized starch defect mutant strain and application thereof | |
| CN106467897A (en) | A kind of rich grease-contained scenedesmus and its culture application | |
| CN103881923A (en) | Method for culturing microalgae by using coking wastewater | |
| CN102746991B (en) | Method for carrying out heterotrophic culture on chlorella with acid-producing wastewater | |
| CN103045478B (en) | Method for culturing oil-producing microalgae with high density and high lipid production rate | |
| CN107460215A (en) | A kind of method of microalgae mixed culture production grease |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190830 |