CN110184223A - A kind of Kayseri arthrobacterium that can efficiently remove ammonia nitrogen in aquaculture wastewater and its application - Google Patents
A kind of Kayseri arthrobacterium that can efficiently remove ammonia nitrogen in aquaculture wastewater and its application Download PDFInfo
- Publication number
- CN110184223A CN110184223A CN201910520704.4A CN201910520704A CN110184223A CN 110184223 A CN110184223 A CN 110184223A CN 201910520704 A CN201910520704 A CN 201910520704A CN 110184223 A CN110184223 A CN 110184223A
- Authority
- CN
- China
- Prior art keywords
- kayseri
- arthrobacterium
- sewage
- ammonia nitrogen
- aerobic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/06—Arthrobacter
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Environmental & Geological Engineering (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The present invention relates to livestock and poultry farm sewage aerobic advanced treating field, a kind of Kayseri arthrobacterium that can efficiently remove ammonia nitrogen in aquaculture wastewater and its application are specifically disclosed.Applicant screened from activated sludge one plant of heterotrophic nitrification-aerobic denitrification bacterium Kayseri arthrobacterium (Arthrobacter Keyseri), the bacterial strain, which is survived and had under the conditions of superelevation ammonia nitrogen concentration (2000 mg/L), certain denitrogenates ability, it is added in wastewater from pig farm, the ammonia nitrogen value in sewage can be greatly lowered, COD, TP, TN, TSS indices decline, which can be used for being prepared into the sewage treatment microbial inoculant of commercialization, and the external source as livestock and poultry farm aerobic sewage treatment technology adds strain, cost of sewage disposal is reduced, sewage harmless treatment purpose is reached.
Description
Technical field
The present invention relates to livestock and poultry farm sewage aerobic advanced treating fields, can efficiently remove in particular to a kind of
The Kayseri arthrobacterium of ammonia nitrogen and its application in aquaculture wastewater.
Background technique
Aquaculture sewage belongs to the high concentration organic sewage rich in a large amount of pathogen, is discharged directly into water body or storing place
Point is improper, is entered water body by rain-out, it would be possible to cause the severe exacerbation of surface water or quality of groundwater.Due to animal dung
The leaching of urine is very strong, and the leaching such as nitrogen, phosphorus and water soluble organic substance in fecaluria are very big, if do not dealt carefully with, will pass through
Rainwash and diafiltration enter water table polluted underground water.Influence to surface water is then mainly shown as, large amount of organic matter
Into after water body, the dissolved oxygen in a large amount of consumption water is kept water body smelly by the decomposition of organic matter；When the dissolved oxygen in water body substantially
After degree decline, large amount of organic matter can continue to decompose under anaerobic, and methane, hydrogen sulfide etc. will be generated in decomposition poison gas
Body leads to aquatile mortality；A large amount of suspended matters in sewage can make water body muddy, reduce the photosynthetic work of algae in water
With, limit the normal activity of aquatile, make it is gradually dead to the aquatile that organic pollution is sensitive, to be further exacerbated by
Water bottom anoxic makes the reduction of water body assimilative capacicy；Nitrogen, phosphorus can make water eutrophication, and the result of eutrophication can make water body
Middle NO3-N and NO2-N excessive concentration, if people and animals' long-term drinking can cause to be poisoned, and the growth of some toxic algaes and big
Amount breeding can discharge a large amount of toxin in water body, lead to the mortality of aquatic animal, to seriously destroy water ecology
Balance；Some germs, virus in fecaluria etc. may cause certain epiphytotics propagation etc. with water flowing.
Livestock and poultry farm sewage disposal technology is broadly divided into Anaerobic Treatment and Aerobic Process for Treatment two major classes at present, and anaerobic technique has
Very much, wherein more there are commonly following several: anaerobic filter (AF), up-flow anaerobic sludge blanket (UASB), composite anaerobic are anti-
Answer device (UASB+AF), two phase anaerobic digestion method and up-flow sludge bed reactor (USR) etc.；Aerobic treatment process can be divided into again
Natural aerobic biological treatment (water body purification, soil sanitation) and the activated sludge process of artificial Aerobic Process for Treatment, biofilter, biology
Turntable, biological contact oxidation process, sequencing batch active sludge (SBR), aerobic/anaerobic (A/O) and oxidation ditch process etc..
If the aquaculture sewage after aerobic process processing is separated by solid-liquid separation is directlyed adopt, although saving than anaerobic treatment process
A large amount of capital construction cost, but since the power consumed in aeration process is big, need huge energy cost still to will cause at sewage
Reason cost still remains high, and is a heavy burden for livestock and poultry cultivation enterprise, reduces this problem of the processing cost of sewage
It is urgently to be resolved.
Kayseri Arthrobacter is a kind of Gram-positive chemoheterotrophic bacteria in Arthrobacter (Arthrobacter sp.).
Arthrobacter bacterium is a kind of generally existing bacterium with higher genetic adaptation, it is widely present in environment.At present
Having Arthrobacter class bacterium to the report of contaminant degradation, such as arthrobacterium (Arthrobacter sp.) YC-RLl, (Yan Yanchun appoints
It is of heap of stone, Shi Yanhua, a kind of arthrobacter bacteria of et al. and its apply), but have no related Kayseri arthrobacterium organic pollutant
Report.
Summary of the invention
It is described the object of the present invention is to provide a kind of Kayseri arthrobacterium that can efficiently remove ammonia nitrogen in aquaculture wastewater
Bacterial strain is sent on January 21st, 2019 to China typical culture collection center preservation, classification naming: Kayseri arthrobacterium
(Arthrobacter Keyseri) A-4, deposit number: CCTCC NO:M2019065, address: Wuhan, China Wuhan University.
It is another object of the present invention to provide Kayseri arthrobacterium (Arthrobacter Keyseri) A-4 in dirt
Application in water process.
In order to reach the goals above, the present invention takes following technical measures:
A kind of screening for the Kayseri arthrobacterium that can efficiently remove ammonia nitrogen in aquaculture wastewater:
Bacterial strain screening of the invention retains ammonia from the activated sludge at Hua Zhong Agriculture University's fine work breeding station sewage treatment end
Nitrogen (NH4+-N) removal rate is up to 70% or more bacterial strain, after 16SrDNA is compared, obtains one plant of Kayseri arthrobacterium, the bacterial strain
It send on January 21st, 2019 to China typical culture collection center preservation, classification naming: Kayseri arthrobacterium
(Arthrobacter Keyseri) A-4, deposit number: CCTCC NO:M2019065, address: Wuhan, China Wuhan University.
The bacterium is gram-positive bacteria, and quarter butt is rodlike, and bacterium colony is in faint yellow opaque spot distribution；Common culture medium can be used
LB, which is placed in shake culture under normal temperature state bacterium solution is made, to be used.
Application of Kayseri arthrobacterium (Arthrobacter Keyseri) A-4 in sewage treatment, including the use of the bacterium
Ammonia-nitrogen content in strain removal sewage, or it is prepared into the sewage-treating agent except ammonia nitrogen；It can be also used for reducing in sewage
COD, TSS, TN, TP etc..
Beneficial effects of the present invention:
The high concentration organic sewage survival that the bacterial strain that the present invention screens can generate during livestock and poultry cultivation；With corresponding
Ratio is added to the aerobic wastewater treatment section after the front-end processing of solid-liquid separation process, improves the heterotrophism nitre in aerobic tank
Change-aerobic denitrification denitrifying bacterium content, accelerates the process denitrogenated, and shortens hydraulic detention time, reduce Sewage advanced treatment at
This.
Detailed description of the invention
Fig. 1 is the growthform and Grain stain figure of Arthrobacter Keyseri A-4 bacterial strain.
Fig. 2 is addition bacterium solution wastewater from pig farm reaction front and back form schematic diagram；
Wherein: O: raw sewage；CT: control group；D: Kayseri arthrobacterium group.
Fig. 3 is wastewater from pig farm advanced treatment process general flow chart.
Specific embodiment
In technical solution of the present invention, the reagent is purchased from biochemical shop, the technical solution is such as if not otherwise specified
It is not specified, be
The ordinary skill in the art.
Sewage indices detection method in test:
COD: dichromic acid alkaline process (GB 11914-89)
NH4 +- N: Berthelot spectrophotometry (GB 7479-87)
TN: alkaline chitinase clears up ultraviolet spectrophotometry (GB 11894-89)
TP: ammonium molybdate spectrophotometric method (GB 11893-89)
TSS: gravimetric method (GB 11901-89)
Thalli growth amount: optical densitometric method (OD600)
Used culture medium and composition proportion in test:
1L nitrifies enriched medium: (NH4)2SO40.5g；Trisodium citrate 4.08g；KH2PO41.0g；Frerrous chloride
0.5g；Magnesium sulfate 1.0g；PH 7.0-7.5 is adjusted, remaining is water.
1L bromthymol blue (BTB) screening and culturing medium: KNO31.0g；Altheine 1.0g；Trisodium citrate 8.5g；
KH2PO41.0g；Frerrous chloride 0.05g；Magnesium sulfate 1.0g；Calcium chloride 0.2g；Agar 20g；With the bromine hundred of alcohol dilution 0.1%
In blue (BTB) the solution 5mL of phenol；PH7.0-7.5 is adjusted, remaining is water.
1L heterotrophic ammonia-oxidizing culture medium: (NH4)2SO40.472g；Trisodium citrate 4.902g；K2HPO40.2g；Magnesium sulfate
0.05g；Ferrous sulfate 0.01g；Manganese sulfate 0.01g；NaCl 0.12g；PH7.0-7.5 is adjusted, remaining is water.
Embodiment 1:
Kayseri arthrobacterium (Arthrobacter Keyseri) A-4's isolating and purifying and identifying:
1, the sampling of activated sludge and acclimating culture
The selection of the facultative lagoon at Hua Zhong Agriculture University's fine work breeding station sewage treatment end and the sampling of activated sludge, will be active
Sludge is added in special aerator, then adds the nitrification enriched medium that two arrive three times and carry out artificial intermittent (every aeration
Stop one hour within three hours) Air Exposure, keep water body dissolved oxygen amount to be not less than 3mg/L.Continuously two months or so time of culture, phase
Between stopping the primary nitrification enrichment culture liquid of aeration time section replacement, (replacement nutrient solution volume accounts for about the 1/ of initial volume daily
50), until culture sludge is converted into khaki by grey black, a period of time water body clear is stood after stopping aeration.
2, the separation screening of heterotrophic nitrification-aerobic denitrification bacterium
It takes the khaki activated sludge 1g in aerator to 50mL centrifuge tube, adds the sterile water of 30mL or so, repeatedly
Acutely concussion forms mixed bacteria liquid.10 are diluted to using coubling dilution-1To 10-7The bacteria suspension of gradient, then use disinfection inoculation
Ring dips bacterium solution and crosses on BTB initial screening solid medium, is inverted culture dish and observes daily in 30 DEG C of constant incubators
Colonial morphology.
Compare picking well-grown and bacterium colony periphery culture medium is made to become blue bacterium colony and is drawn in BTB secondary screening solid medium
Line isolates and purifies, and is inverted culture dish in 30 DEG C of constant incubators and observes colonial morphology daily until obtain the consistent bacterium colony of feature,
Then picking colony repeats previous step scribing line isolation and purification culture, until the colony morphology characteristic obtained every time is consistent, it will not
There are other miscellaneous bacteria bacterium colonies.
3, bacterium performance Preliminary Determination
Obtained strains disinfection inoculation ring is chosen and is seeded to sterilized nitrification culture medium, shake culture is for a period of time extremely
Nitrifying culture medium muddiness completely, (effective bacterial content is 108-109cfu/mL)；Bacterium will be cultivated by certain volume ratio (1:100)
Liquid is added to the TN, ammonia nitrogen (NH of measurement culture solution after heterotrophic ammonia-oxidizing culture medium shake culture 48h4 +- N) value, retain ammonia nitrogen
(NH4 +- N) removal rate is up to 70% or more bacterial strain.
4, the identification of bacterial strain
It is finally obtained one plant of bacterium with ammonia nitrogen removal function, the DNA of the bacterial strain is extracted, passes through PCR amplification
16SrDNA segment, after sequencing by DNA sequence dna GeneBank carry out BLAST comparison, the bacterial strain DNA sequence dna compare with
Kayseri arthrobacterium (Arthrobacter Keyseri) similarity is up to 99%.
The bacterium is gram-positive bacteria, and quarter butt is rodlike, and bacterium colony is in faint yellow opaque spot distribution (shown in Fig. 1), is had
Produce alkali ability.According to bacterial growth morphological feature referring to " primary Jie Shi systematic bacteriology handbook " and " common bacteria system identification hand
Volume " physiological and biochemical property that meets Bacillus bacteria, it is determined as Kayseri arthrobacterium.
Above-mentioned bacterial strains are sent on January 21st, 2019 to China typical culture collection center preservation, classification naming: opening plug
Sharp arthrobacterium (Arthrobacter Keyseri) A-4, deposit number: CCTCC NO:M2019065, address: Wuhan, China is military
Chinese university.
Embodiment 2:
The culture of Kayseri arthrobacterium (Arthrobacter Keyseri) A-4 or fermentation method:
Configure routine LB culture medium (tryptone (Tryptone) 10g/L, yeast extract (Yeast extract) 5g/
L, sodium chloride (NaCl) 10g/L
After adjusting pH to 7.0) high pressure steam sterilization, it is inoculated with Kayseri arthrobacterium in superclean bench, is placed in 25 DEG C,
Completely muddy to culture solution after shake culture 48h under the conditions of 120r/min, the effective bacterial content of culture solution reaches 10 at this time8cfu/
mL。
Embodiment 3:
Growth effect of the reaction condition to Kayseri arthrobacterium (Arthrobacter Keyseri) A-4:
1, common factors grow and are denitrogenated on bacterium performance and influence
In view of may be subjected to weather temperature in bacterial strain practical application, pH value of aeration quantity and water inlet etc. is to Kayseri section
The influence of the growth performance and efficiencies of nitrogen removal of bacillus, spy are arranged this group and test to test the best growing condition of bacterium, are Kayseri
In the aerobic advanced treatment process of piggery wastewater, preferably application provides corresponding term of reference to arthrobacterium.This test setting influence because
Son is as follows: temperature (20 DEG C, 30 DEG C, 40 DEG C) and revolving speed (60r/min, 120r/min, 240r/min) and pH (5,6,7,8,9)
Value, designs corresponding test group growth conditions by control variate method；It tests used medium and measures initial ammonia nitrogen (NH4 +- N) it is dense
Degree is about 110.00mg/L, and total nitrogen (TN) concentration is about 120.0mg/L, and pH value is 7.00 or so.
1 temperature of table, revolving speed, pH grow bacterium and performance influences
Note: ammonia nitrogen, total nitrogen unit mg/L, T, R, P respectively represent tri- kinds of temperature, revolving speed, pH factors to the growth of bacterium
It can influence, subscript number represents the different level of each factor；Superscript letter represents the same row difference factor and is compared to each other, no
Significant difference (P < 0.05) is indicated with letter.
T1~T3, R1~R3, P1~P5 respectively represent temperature (20 DEG C, 30 DEG C, 40 DEG C) and revolving speed (60r/min, 120r/
Min, 240r/min) and three kinds of factor different levels of pH (5,6,7,8,9)
Test and analyze us by test data and obtain following result: Kayseri arthrobacterium denitrogenates performance the best growing condition
Respectively temperature is 30 DEG C, revolving speed 120r/min, pH 7, and the 36h ammonia nitrogen concentration of concussion processing at this time is dropped to by 110.0mg/L
6.4mg/L, removal rate is up to 94.2%.
2, wastewater with high ammonia nitrogen concentration influences bacterium growth performance
Piggery wastewater is high concentration organic sewage, without the piggery wastewater that front end is separated by solid-liquid separation, high with organic concentration,
The typical " three high " feature that suspended matter is more, ammonia-nitrogen content is high, i.e. 3000~12000mgL of COD-1, ammonia nitrogen 800~
2200mg·L-1, the features such as TSS exceeded decades of times.Wherein there are tremendous influences to bacterium growth for ammonia nitrogen.Mainly test is opened for this test
Sharp arthrobacterium is filled in the tolerance of ammonia nitrogen concentration, and for Kayseri arthrobacterium, preferably application provides corresponding term of reference.
Test setting ammonia nitrogen concentration is respectively as follows: five groups of 200mg/L, 400mg/L, 800mg/L, 1600mg/L, 2000mg/L,
Taking the culture solution of Kayseri arthrobacterium, (effective bacterial content is 108Cfu/mL) it is added to pig by certain volume ratio (1:100)
In the waste water of field, room temperature shake culture 36h, the density (OD of detection bacterium growth600) value and other indices.
The ammonia-nitrogen content of 2 various concentration of table grows bacterium and performance influences
Note: ammonia nitrogen, total nitrogen unit mg/L；N, which represents ammonia nitrogen concentration, influences the growth performance of bacterium, N1~N5It respectively corresponds
Ammonia nitrogen concentration is the piggery wastewater of 200mg/L, 400mg/L, 800mg/L, 1600mg/L, 2000mg/L, and subscript number represents
The different level of each factor；Superscript letter represents the same row difference factor and is compared to each other, and different letters indicate that difference is aobvious
It writes (P < 0.05).
It, can be in ammonia nitrogen concentration from above data it is found that Kayseri arthrobacterium has very strong resistance to high-concentration ammonia-nitrogen ability
Up to growing in the environment of 2000mg/L, and there is certain ammonia nitrogen rate of going out, in the environment item that ammonia nitrogen concentration is 200mg/L-800mg/L
Under part, ammonia nitrogen removal frank is more than 70%.Embodiment 3:
Kayseri arthrobacterium (Arthrobacter Keyseri) A-4 measures piggery wastewater actual processing effect:
1, laboratory lab scale
Taking the culture solution of Kayseri arthrobacterium, (effective bacterial content is 108Cfu/mL certain volume ratio (1:100)) is pressed
It is added in piggery wastewater, (25-30 DEG C) of room temperature concussion (revolving speed is 120 turns/min) cultivates 36h, and the items for measuring waste water refer to
Mark；Not add the identical waste water of bacterium solution as control group.
Piggery wastewater is derived from the water inlet end of Hua Zhong Agriculture University's breeding station sewage treatment facility, visually observes shape before and after the processing
State is as follows:
Comparison reaction front and back wastewater from pig farm form, from Fig. 2 this it appears that reaction before wastewater from pig farm (O) in ash
There is foam on the opaque shape of cyan, surface layer, and bottom has contamination precipitation after standing；It is inoculated with Kayseri arthrobacterium and passes through 36h shake culture
Afterwards, it can clearly find out that wastewater from pig farm becomes as clear as crystal shape, the still end in surface layer by the opaque shape of grey cyan, bottom has largely
Grey cyan flocculent deposit formed, specific targets change see table 3.
Table 3
Note: data unit is mg/L in table, and national standard refers to " livestock and poultry breeding industry pollutant emission standard (GB 18596-
2001)"；Test group and initial concentration are compared, different letters indicate significant difference (P < 0.05).
It can be obtained from test data analyzer, the removal rate that Kayseri arthrobacterium bacterium solution piggery wastewater indices are added is above
Bacterium solution control group is not added, by 36h oscillation treatment, the ammonia nitrogen (NH of test group4 +- N) value drops to by 225.8mg/L or so
55.2mg/L, rate of descent are up to about 75%, since Kayseri arthrobacterium is there are also certain flocculation ability, total suspended matter in waste water
(TSS) concentration rate of descent is about 69%, and two indexs are up to livestock and poultry breeding industry National Emission Standard (GB18567-
2001)。
2, wastewater from pig farm A2/O processing system pilot scale
This test site covers pre-treatment in a certain large―scale hoggery in Wuhan City, Hubei Province, this sewage treatment process scheme
System (separating dry space from moist space system), anaerobic methane system, depth A2/O biological treatment system and oxidation pond aquatic plant purification system,
Four is mutually auxiliary, and building ecological energy uniformly utilizes circulation pattern, and reaction process is as shown in Figure 3.
Kayseri pole bacterial solution (1:10000 is added by volume) to secondary anaerobic pond (micro- exposure) is added by outer rim
In aerobic tank, by the reaction of 48h, water quality (COD, NH of water inlet end and water outlet are detected4 +-N、TP、TN)。
Sewage disposal system passes in and out water end (W.E.) water quality result before 4 external source of table adds bacterium solution
Note: data unit is mg/L in table.
Sewage disposal system passes in and out water end (W.E.) water quality result after 5 external source of table adds bacterium solution
Note: data unit is mg/L in table.
It can be found by data in contrast table 3-2 and 3-3, Kayseri arthrobacterium bacterium solution water outlet sewage is added by external source
COD, NH4 +Before-N, TP, TN indices rate of descent are above addition, wherein NH4 +- N removal rate adds bacterium solution group and is higher than not
Addition group nearly 10%.
Claims (5)
1. a kind of isolated Kayseri arthrobacterium (Arthrobacter Keyseri), the bacterial strain is named as Kayseri pole
Bacterium A-4, deposit number are as follows: CCTCC NO:M2019065.
2. application of the Kayseri arthrobacterium described in claim 1 in sewage treatment.
3. Kayseri arthrobacterium described in claim 1 is preparing the application in sewage-treating agent.
4. application according to claim 2 or 3, the Kayseri arthrobacterium is for reducing the ammonia-nitrogen content in sewage.
5. application according to claim 2 or 3, the Kayseri arthrobacterium for reducing in sewage total nitrogen, total phosphorus,
COD or total suspended matter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910520704.4A CN110184223B (en) | 2019-06-17 | 2019-06-17 | Arthrobacter calsium capable of removing ammonia nitrogen in culture sewage and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910520704.4A CN110184223B (en) | 2019-06-17 | 2019-06-17 | Arthrobacter calsium capable of removing ammonia nitrogen in culture sewage and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110184223A true CN110184223A (en) | 2019-08-30 |
| CN110184223B CN110184223B (en) | 2020-11-24 |
Family
ID=67722037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910520704.4A Active CN110184223B (en) | 2019-06-17 | 2019-06-17 | Arthrobacter calsium capable of removing ammonia nitrogen in culture sewage and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110184223B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112940995A (en) * | 2021-04-28 | 2021-06-11 | 武汉大学 | Arthrobacter capable of degrading n-eicosane and application thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87100664A (en) * | 1986-01-09 | 1987-08-26 | 伏尔加—乌拉尔含硫化氢天然气开采和加工科研设计院 | Biological purification of waste water is removed the method for ethylene glycol |
| CN102604870A (en) * | 2012-03-26 | 2012-07-25 | 浙江师范大学 | Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof |
| CN104164390A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝天成生物科技有限公司 | Arthrobacter nicotianae and application thereof in aquaculture |
| CN104862260A (en) * | 2015-06-10 | 2015-08-26 | 国家海洋局第三海洋研究所 | Arthrobacter with aerobic denitrification capability and application thereof |
| CN104946556A (en) * | 2015-05-15 | 2015-09-30 | 中国农业科学院研究生院 | Arthrobacter and application thereof |
| CN105713862A (en) * | 2016-03-28 | 2016-06-29 | 武汉科技大学 | Bacterial strain capable of degrading pyridine and ammonia nitrogen, preparation method and application of bacterial strain |
| CN106399191A (en) * | 2016-10-31 | 2017-02-15 | 杭州朗境环保科技有限公司 | Strain of Arthrobacter sp. B2 and application thereof to nitrogen-containing sewage degradation |
| CN106591181A (en) * | 2016-12-08 | 2017-04-26 | 河北大学 | Arthrobactermysorens and application thereof to purification of marine-culture nitrogen-containing wastewater |
| CN106754576A (en) * | 2017-03-10 | 2017-05-31 | 江南大学 | The bacterial strain of nitrogen and its application in one plant of fast degradation sewage |
| CN107245463A (en) * | 2017-06-22 | 2017-10-13 | 温州大学 | Heterotrophic nitrification-aerobic denitrification arthrobacter and application thereof |
-
2019
- 2019-06-17 CN CN201910520704.4A patent/CN110184223B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87100664A (en) * | 1986-01-09 | 1987-08-26 | 伏尔加—乌拉尔含硫化氢天然气开采和加工科研设计院 | Biological purification of waste water is removed the method for ethylene glycol |
| CN102604870A (en) * | 2012-03-26 | 2012-07-25 | 浙江师范大学 | Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof |
| CN104164390A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝天成生物科技有限公司 | Arthrobacter nicotianae and application thereof in aquaculture |
| CN104946556A (en) * | 2015-05-15 | 2015-09-30 | 中国农业科学院研究生院 | Arthrobacter and application thereof |
| CN104862260A (en) * | 2015-06-10 | 2015-08-26 | 国家海洋局第三海洋研究所 | Arthrobacter with aerobic denitrification capability and application thereof |
| CN105713862A (en) * | 2016-03-28 | 2016-06-29 | 武汉科技大学 | Bacterial strain capable of degrading pyridine and ammonia nitrogen, preparation method and application of bacterial strain |
| CN106399191A (en) * | 2016-10-31 | 2017-02-15 | 杭州朗境环保科技有限公司 | Strain of Arthrobacter sp. B2 and application thereof to nitrogen-containing sewage degradation |
| CN106591181A (en) * | 2016-12-08 | 2017-04-26 | 河北大学 | Arthrobactermysorens and application thereof to purification of marine-culture nitrogen-containing wastewater |
| CN106754576A (en) * | 2017-03-10 | 2017-05-31 | 江南大学 | The bacterial strain of nitrogen and its application in one plant of fast degradation sewage |
| CN107245463A (en) * | 2017-06-22 | 2017-10-13 | 温州大学 | Heterotrophic nitrification-aerobic denitrification arthrobacter and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| QINGFENG WANG 等: "Isolation and characterization of a high-efficiency soil atrazine-degrading Arthrobacter sp strain", 《INTERNATIONAL BIODETERIORATION & BIODEGRADATION》 * |
| QINGMING ZHANG 等: "Impacts of nitrogen and phosphorus on atrazine-contaminated soil remediation and detoxification by Arthrobacter sp. strain HB-5", 《ENVIRON EARTH SCI》 * |
| 司文攻 等: "耐受高浓度氨氮异养硝化菌的筛选及其脱氮条件优化", 《环境科学》 * |
| 陈燕 等: "1株高效聚磷节杆菌的分离鉴定及其聚磷特性研究", 《安徽农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112940995A (en) * | 2021-04-28 | 2021-06-11 | 武汉大学 | Arthrobacter capable of degrading n-eicosane and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110184223B (en) | 2020-11-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lin et al. | Use of ammoniacal nitrogen tolerant microalgae in landfill leachate treatment | |
| CN103911332B (en) | A kind of method of cultivation for black and odorous water denitrogenation dephosphorizing composite bacteria | |
| CN101302485B (en) | Heterotrophic nitrification microbial preparation, cultivation method and use thereof | |
| CN106754576B (en) | The bacterial strain of nitrogen and its application in one plant of fast degradation sewage | |
| CN109943497A (en) | One plant of Pseudomonas stutzeri and application thereof with aerobic denitrification function | |
| CN102443558B (en) | Composite heterotrophic nitrifying bacterial agent and application of same in nitrogen removal treatment of waste water containing ammonia and nitrogen | |
| US20030211594A1 (en) | Microalgae for remediation of waste and method of culturing the same | |
| CN105255782B (en) | There is fiber bacterium and the purposes of reducing power to Cr VI | |
| CN105174471A (en) | Reinforced black odorous river microecological reconstruction/balance and water quality improvement method | |
| CN114890555B (en) | Solid microbial preparation for treating rural black and odorous water body and preparation method and application thereof | |
| CN109678257A (en) | A method of being suitable for the functional flora construction method and application flora improvement black and odorous water of black and odorous water improvement | |
| CN109110912A (en) | A kind of dirt collecting pit pool black and odorous water administering method | |
| CN111733113B (en) | COD (chemical oxygen demand) degrading strain and application thereof | |
| CN109052654A (en) | A method of for improving the smelly water of urban black | |
| CN103103153A (en) | Anaerobic denitrification phosphorus-accumulating bacteria strain with denitrification and phosphorous removal effects and application thereof | |
| CN114703095A (en) | Pseudomonas mendocina and application thereof in field of sewage and wastewater purification | |
| CN105585210A (en) | Novel technology for treating aquaculture wastewater of fungus-aquatic plant symbiotic system | |
| CN109439569A (en) | Heterotrophic nitrification-biological aerobic denitrification comamonas, the liquid bacterial agent containing the bacterium and its application in membrane bioreactor | |
| CN113151063B (en) | Citrobacter freundii AS11 and application thereof in sewage treatment | |
| CN110184223A (en) | A kind of Kayseri arthrobacterium that can efficiently remove ammonia nitrogen in aquaculture wastewater and its application | |
| CN102703361A (en) | Facultative anaerobic denitrifying bacterium with self-flocculation capability and purpose thereof | |
| CN115353210B (en) | Application of bacillus pumilus LZP02 in treatment of pig raising wastewater | |
| CN110184217A (en) | One plant using nitrite as the salt tolerant denitrifying bacteria of nitrogen source and its application | |
| CN110104798A (en) | A kind of complex microorganism preparations and application for sewage treatment | |
| Hong | Growth of Spirulina platensis in effluents from wastewater treatment plant of pig farm |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |