CN110184071A - A kind of alkaline land soil microbial growth agent and its application - Google Patents
A kind of alkaline land soil microbial growth agent and its application Download PDFInfo
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- CN110184071A CN110184071A CN201910350145.7A CN201910350145A CN110184071A CN 110184071 A CN110184071 A CN 110184071A CN 201910350145 A CN201910350145 A CN 201910350145A CN 110184071 A CN110184071 A CN 110184071A
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Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The invention discloses a kind of alkaline land soil microbial growth agent and its application, the alkaline land soil microbial growth agent is made of urase, catalase, deoxyribonuclease, carrotene, acetylcholine, medical stone and humic acid；Each component mass percent is respectively urase 0.05-4.0%, catalase 0.05-4.0%, deoxyribonuclease 0.05-4.0%, carrotene 1.0-4.0%, acetylcholine 0.05-4.0%, medical stone 45.0-55.0%, humic acid 50.0-65.0%；The sum of each component gross mass percentage composition of alkaline land soil microbial growth agent is 100.0%.The edaphon enrichment procedure can improve functional microorganism diversity and abundance in alkaline land soil, improve soil texture, promote plant and soil to nitrogen fixation, promote soil enzyme system balancing, and then promote soil and train raw ability.
Description
Technical field
The invention belongs to salt affected soils to improve field, be related to a kind of alkaline land soil microbial growth agent and its application.
Background technique
Soil is one of the fundamental of the composing environment ecosystem, is material base for the survival of mankind, has one
Determine fertility, is capable of the weaker zone of growing plant.Soil minerals, animals and plants as made of rock weathering and microorganism residuum decomposition
Organic matter, geobiont (solid matter) and the moisture (liquid phase substance) of generation, air (gaseous substance), the humus aoxidized
Deng composition.This three categories substance constitutes a unity of contradictions body in soil.They are interknited, and are conditioned each other, and are crop
Required living condition is provided, is the material base of soil fertility.Soil be land plant raw base, be that the mankind are engaged in agriculture
The material base of industry production.
Salt-soda soil is a kind of soil types widely distributed on the earth, is a kind of important land resource.Saline Land
Problem is closely related with mankind's activity, not only causes the destruction of resource, brings massive losses to agricultural production, and limitation is entirely
One of ball economic growth and the most important abiotic stress of crop production, and constitute a threat to biosphere and ecological environment,
Show harm of both economy and environment.The interpromoting relation in five elements in addition, salination process often accompanies with Desertification Process, or even mutually promote
Into mutually converting.The formation of China's alkaline earth and alkali-affected soil, it is most of related with the accumulation of Carbonates In Soil, thus alkalize
Degree is universal higher, and serious salt affected soil regional plant can hardly survive.
Soil salt content is one of the major parameter for characterizing soil regime, and determines the most important of soil salinization degree
Index.After soluble-salt contained in soil reaches certain amount, will have a direct impact on the normal growth of crop, and soluble salt because
It forms difference, also different to the crop extent of injury, and the salt resistance ability of this and various crops also has substantial connection certainly.
Cultivated area such a for China is few, and the problems such as with population growth, land deterioration, shortage of land resource
Exacerbation, exploitation saline-alkali soil just become particularly significant, can not only alleviate crisis in food, moreover it is possible to improve the ecological environment, improve people
Quality of life, quickly exploitation and efficiently utilize salinization soil, economic, environmental protection and sustainable development can be promoted well
Exhibition has vital practice significance.
According to the formation and feature in salt-soda soil, promote the measure of alkaline land improving very much, have water conservancy, physical chemistry, agronomy,
Biological control measure etc., thinking and technology are also constantly developing.In each scientific method for promoting salt affected soil improvement, biology changes
It is good to assert it is one of most effective approach by extensive.Accumulation of the activity of geobiont to the soil organism, available nutrient
Improvement with soil texture has a degree of impetus.
Soil is naturally occurred during earth evolution, is the important composition of ecological environment and the mankind are rely life
The main carriers deposited, and microorganism is then most active composition in soil, edaphon takes part in from cyanobacteria, green alga and is colonized in
Parent soil material starts all processes for gradually arriving the soil development of soil fertility formation.Edaphon is maintaining the ecosystem whole
It is played an important role in terms of body service function, is often compared to " converter ", the terrestrial ecosystems of soil nutritive element circulation
Stable " adjuster ", while soil provides good physical structure and chemotrophy (Song again for microorganism growth and breeding
It is green for a long time etc., 2013).Soil biology character (microbial populations in soil, structure of community and its functional group, microbial biomass, enzymatic activity
Deng) it can reflect the differentiation of soil quality, soil fertility, and the Biological indicators for the soil health that judges can be used.
Summary of the invention
The present invention is directed in view of the deficiencies of the prior art, provide a kind of alkaline land soil microbial growth agent.
It is a further object of the present invention to provide the preparation methods of the alkaline land soil microbial growth agent.
It is yet another object of the invention to provide the applications of the alkaline land soil microbial growth agent.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of alkaline land soil microbial growth agent, the alkaline land soil microbial growth agent is by urase, peroxidating
The composition such as hydrogen enzyme, deoxyribonuclease, carrotene, acetylcholine, medical stone and humic acid；Wherein urease activity >=8u/
Mg, catalase activity >=16u/mg, deoxyribonuclease activity >=16u/mg, each component mass percent are respectively urea
Enzyme 0.05-4.0%, catalase 0.05-4%, deoxyribonuclease 0.05-4.0%, carrotene 1-4.0%, acetyl
Choline 0.05-4.0%, medical stone 45.0-55.0%, humic acid 50.0-65.0%；Alkaline land soil microbial growth agent it is each
The sum of component gross mass percentage composition is 100.0%.
As a preference of the present invention, urase, catalase, deoxidation core in the alkaline land soil microbial growth agent
Ribonuclease T., carrotene, acetylcholine, medical stone and humic acid mass percent successively account for 1.0-2.0%, 0.05- respectively
1.0%, 0.05-1.0%, 1-2.0%, 0.05-4.0%, 45.0-50.0% and 50.0-55.0%.
The medical stone powder partial size is 65-250 mesh, preferably 65-115 mesh.
Alkaline land soil microbial growth agent of the present invention, is mainly prepared by the following method to obtain: by humic acid
It is stirred evenly according to the above ratio with medical stone；By the urase of formula ratio, catalase, deoxyribonuclease, carrotene
And acetylcholine, be added in the mixture of medical stone and humic acid and be sufficiently stirred while stirring, make urase, catalase,
Deoxyribonuclease, carrotene and acetylcholine are uniformly attached on medical stone under the dilution of humic acid, are then made
Pulvis or granule obtain the alkaline land soil microbial growth agent.
As a preference of the present invention, the agent of alkaline land soil microbial growth will seal, prevent moisture from evaporating.
The preparation method of the alkaline land soil microbial growth agent, including according to the above ratio by humic acid and medical stone
It stirs evenly；By the urase of formula ratio, catalase, deoxyribonuclease, carrotene and acetylcholine, while stirring
It is added in the mixture of medical stone and humic acid and is sufficiently stirred, make urase, catalase, deoxyribonuclease, Hu Luo
Bu Su and acetylcholine are uniformly attached on medical stone under the dilution of humic acid, be then made pulvis or granule obtain it is described
Alkaline land soil microbial growth method.
Application of the alkaline land soil microbial growth agent of the present invention in salt affected soil improvement.
A method of promoting alkaline land soil microbial growth, is applied into alkaline land soil of the present invention saline and alkaline
Ground edaphon multiplication agent.
The utility model has the advantages that
A kind of alkaline land soil microbial growth agent provided by the present invention, has the following advantages that and good effect:
1) urase is present in soil, bacterium, fungi and higher plant, enzymatic preparation --- ammonia be plant nitrogen source it
One.Urease activity reacts SOIL ORGANIC NITROGEN to the conversion capability of available nitrogen and the deliverability of inorganic nitrogen in soil, activity with
Soil microbe quantity, organic matter content, full nitrogen and available phosphorus contents are positively correlated, and general neutral soil urease activity is greater than
Basic soil.People often characterize soil nitrogen situation with soil urease liveness.Hydrogen peroxide is widely present in soil and biology
In vivo, there is toxic action to biology and soil, catalase can reduce the poison of hydrogen peroxide with peroxynitrite decomposition hydrogen
Evil effect.The overall activity of the active reaction edaphon metabolism of catalase, can be used as microbiological oxidation also
The index of proper energy power, activity are related with soil organic matter content, micro organism quantity.Carrotene has scavenging activated oxygen
Ability is a kind of good antioxidant and oxidation protection agent, microorganism or plant can be allowed to resist oxidation under adverse circumstance environment
Stress.Acetylcholine can cause the increase of cell oxygen consumption rate and the increase to calcium uptake.Medical stone has porosity, surface area
It is very big, there is very strong suction-operated and exchange interaction.Preparation method is combined into one and has above-mentioned each component through the invention
Machine is whole, and each component combination, support of working in coordination between each other can improve microbial diversity in alkaline land soil, change
Kind soil texture promotes plant and soil to nitrogen fixation, promotes soil enzyme system balancing, and then promotes soil and train raw ability,
Its technical effect is more superior than the synthesis of each component effect.
Detailed description of the invention
The Alpha diversity indices box of Fig. 1 bacterium (16s rDNA) schemes
Histogram of Fig. 2 bacterium (16s rDNA) in door level
The Alpha diversity indices box of Fig. 3 fungi (ITS) schemes
Fig. 4 fungi analyzes histogram in the species composition for belonging to (ITS) in level
Fig. 5 applies (10 days) soil Contrast on effect before and after salt-soda soil edaphon multiplication agent；
Fig. 6 applies (25 days) soil Contrast on effect before and after salt-soda soil edaphon multiplication agent；
Fig. 7 applies the soil crust situation (left side: skinning after salt-soda soil edaphon multiplication agent；It is right: skinning knot under Electronic Speculum
Structure).
Specific embodiment
Embodiment 1:
A kind of alkaline land soil microbial growth agent, by urase, catalase, deoxyribonuclease, carrotene,
The composition such as acetylcholine, medical stone and humic acid；Wherein urease activity >=8u/mg, catalase activity >=16u/mg, deoxidation
Ribonuclease activity >=8u/mg, urase in alkaline land soil microbial growth agent, catalase, deoxyribonuclease,
Carrotene, acetylcholine, medical stone (powder diameter is 100 mesh) and humic acid mass percent successively account for salt affected soil increasing respectively
Imitate 1.0%, 0.5%, 0.25%, 1.0%, 0.25%, 47.0% and the 50% of modifying agent.Manufacture craft is as follows: by salt-soda soil
Humic acid and medical stone stir evenly according to the above ratio in edaphon multiplication agent；By the urase of formula ratio, catalase,
Deoxyribonuclease, carrotene and acetylcholine are added in the mixture of medical stone and humic acid sufficiently while stirring
Stirring, makes urase, catalase, deoxyribonuclease, carrotene and the second in alkaline land soil microbial growth agent
Phatidylcholine is uniformly attached on medical stone under the dilution of humic acid, and pulvis is then made or granule obtains the salt-soda soil soil
Earth microbial growth agent.Product needs to seal, to prevent water point evaporation.
Embodiment 2:
Using the alkaline land soil microbial growth agent of embodiment 1, the test of basin alms bowl is carried out, salt content is housed about in basin alms bowl
The agent of alkaline land soil microbial growth is added in 8 ‰ soil treatment group, and additional amount is the 3% of soil weight, is stirred evenly, and marks
For TR；Control treatment is set simultaneously, in addition to not applying the microbial growth agent, remaining condition is substantially identical as processing group, label
For CK.Soil is acquired after 90 days respectively, each processing takes 3 samples to repeat as test at random, carries out microbial diversity
Detection.
Fig. 1 be the microbiologic population reflected by the diversity analysis (Alpha diversity) to monocyte sample abundance and
Diversity.Chao1 index is bigger, shows that the richness of group is higher；The value of Shannon and PD_whole_tree index is higher,
Show that the diversity of group is higher；There are also sequencing depth factor Observed spieces to represent the intuitive quantity statistics of OTUs,
Bacterial community diversity and richness in the soil (TR) of alkaline land soil microbial growth agent, which can be obtained being applied with, all to be had
Reduce significantly.
The bacterium door information of relative abundance top19 is shown in Fig. 2, abscissa is that species are relatively rich in the sample
Degree, ordinate is sample names.Left side Hierarchical clustering analysis between soil sample, the difference between sample is smaller, and sample will
It is in similar same branch, and three parallel samples of CK and TR are all respectively in same branch in figure, but between two groups
It is separation；The right is the structure of community histogram of sample, shows that the structure of community of microorganism in sample, different colours represent
Different plant species.Be applied in the soil (TR) of alkaline land soil microbial growth agent as seen from the figure have significant two it is excellent
Gesture bacterium door --- Proteobacteria Proteobacteria and Firmicutes Firmicutes, and Proteobacteria is in TR and CK
Content accounting in group is respectively that content accounting of 42%, 27%, the Firmicutes in TR and CK group is respectively 47%, 6%,
And content of the Actinobacteria bacterium door detected in CK and TR is respectively 10% and 67%.This absolutely proves salt-soda soil
Edaphon multiplication agent changes the community composition and structure of bacterium in salt affected soil.All Proteobacteria bacteriums are gram
Negative bacterium, there is also anaerobic bacterias, existing autotrophic type also heterotroph for existing aerobic bacteria in the door, and both having there is luminous energy type, there is also changes
It can type.Therefore, the species in Proteobacteria have significant application value in the fields such as agricultural, industry, medicine, health, environmental protection, extensively
It is general to promote utilization, plant pest management, industrial and agricultural wastewater processing, soil remediation and complicated contaminant degradation etc. for nitrogenous fertilizer.
Firmicutes majority is Gram-positive, and many firmicutes can produce gemma, it can resist dehydration and extreme environment, there is one
Class firmicutes --- sun Bacteriaceae can also generate energy by photosynthesis.
Fig. 3 be the microbiologic population reflected by the diversity analysis (Alpha diversity) to monocyte sample abundance and
Diversity.Either depth factor Observed spieces is still sequenced in Chao1, Shannon and PD_whole_tree index
The intuitive quantity statistics of OTUs are represented, the difference of TR and CK group is all unobvious, illustrates to be applied with alkaline land soil microbial growth
Fungal colonization diversity and richness do not have significant change in the soil (TR) of agent.
The Pseudomonas information of relative abundance top17 is shown in Fig. 4, abscissa is sample names, and ordinate is that species exist
Relative abundance in the sample.The fungi being applied in the soil (TR) of alkaline land soil microbial growth agent as seen from the figure
Group's composition has apparent difference with CK.Aspergillus aspergillus, Cladosporium Cladosporium and
Abundance of the Scopulariopsis scopulariopsis in TR soil is significantly higher than CK group, and the dark mycosphaerella of Phaeosphaeria and
The abundance of unidentified Pseudomonas is then that TR group is substantially less than CK group.The change of fungal colonization composition and abundance so not only may be used
To promote the formation of soil agreegate, improves soil texture, Litter-fall etc. in soil can also be accelerated available to animals and plants
Organic matter transformation.
In conclusion Community composition and function of such alkaline land soil microbial growth method to microorganism in salt affected soil
Energy bacterial abundance has significant impact, so that remarkable result is played in the improvement to marine solonchak.
Embodiment 3:
A kind of alkaline land soil microbial growth agent, by urase, catalase, deoxyribonuclease, carrotene,
The composition such as acetylcholine, medical stone and humic acid；Wherein urease activity >=8u/mg, catalase activity >=16u/mg, deoxidation
Ribonuclease activity >=16u/mg, urase, catalase, DNA in alkaline land soil microbial growth agent
Enzyme, carrotene, acetylcholine, medical stone (powder diameter is 80 mesh) and humic acid mass percent successively account for salt affected soil respectively
1.5%, 0.25%, 0.5%, 0.75%, 0.5%, 45.0% and the 51.5% of synergy modifying agent.Manufacture craft is as follows: will be rotten
It grows acid and medical stone stirs evenly according to the above ratio；By the urase of formula ratio, catalase, deoxyribonuclease, Hu Luo
Bu Su and acetylcholine are added in the mixture of medical stone and humic acid while stirring and are sufficiently stirred, and make urase, hydrogen peroxide
Enzyme, deoxyribonuclease, carrotene and acetylcholine are uniformly attached on medical stone under the dilution of humic acid, are then made
The alkaline land soil microbial growth agent is obtained at pulvis or granule.Product needs to seal, to prevent water point evaporation.
Embodiment 4:
Urase, catalase, deoxyribonuclease, carrotene, acetyl in alkaline land soil microbial growth agent
Choline, medical stone (powder diameter be 80 mesh) and humic acid mass percent successively account for 1.75% respectively, 0.75%, 0.15%,
0.2%, 0.15%, 45.0% and 52.0%.Alkaline land soil microbial growth agent is prepared using the production method of embodiment 3.
Field experiment is carried out with the alkaline land soil microbial growth agent of preparation, Jiangsu Province's East Platform strip mud that is experimental field located at is cultivated
Area, test plant are rice, choose area similar in two soil physico-chemical properties, and the application concentration of multiplication agent is 50kg/ mus, simultaneously
It sets blank control B (0kg/ mus).Experimental plot skinning after being applied 10 days and 25 days by analysis edaphon multiplication agent is raw
Long situation and electron-microscope scanning result characterize edaphon multiplication agent to the improved effect of beach salty soil.
Test gained skinning is Biological Soil Crusts.As shown in figure 5, soil table after the application of edaphon multiplication agent 10 days
Face has preliminarily formed the structure of skinning, and soil surface skinning is via a certain amount of liverwort, algae, fungi after application 25 days
With the biotic components (Fig. 6) such as bacterium.By sampling, observation and the analysis to experimental field Biological Soil Crusts, it can be found that skinning
Electron microscope (Fig. 7) in poroid or netted, this structure substantially increases the water conservation and infiltration efficiency of soil.To sum up analysis can
To find out, such edaphon multiplication agent is fairly obvious to the improved effect of beach salty soil.
Claims (8)
1. a kind of alkaline land soil microbial growth agent, it is characterised in that the alkaline land soil microbial growth agent is by urea
Enzyme, catalase, deoxyribonuclease, carrotene, acetylcholine, medical stone and humic acid composition；Wherein urase is living
Property >=8u/mg, catalase activity >=16u/mg, deoxyribonuclease activity >=16u/mg, each component mass percent
Respectively urase 0.05-4.0%, catalase 0.05-4.0%, deoxyribonuclease 0.05-4.0%, carrotene
0.1-4.0%, acetylcholine 0.05-4.0%, medical stone 45.0-55.0%, humic acid 50.0-65.0%, and each component quality
The sum of percentage composition is 100.0%.
2. alkaline land soil microbial growth agent according to claim 1, it is characterised in that the alkaline land soil is micro-
Urase, catalase, deoxyribonuclease, carrotene, acetylcholine, medical stone and humic acid matter in biological multiplication agent
Amount percentage successively accounts for 1.0-2.0%, 0.05-1.0%, 0.05-1.0%, 0.1-2.0%, 0.05-4.0%, 45.0- respectively
50.0% and 50.0-55.0%.
3. alkaline land soil microbial growth agent according to claim 1, it is characterised in that the medical stone powder partial size
For 65-250 mesh.
4. alkaline land soil microbial growth agent described in claim 1, it is characterised in that the alkaline land soil microorganism
Multiplication agent is mainly prepared by the following method to obtain: humic acid and medical stone are stirred evenly in proportion；By the urase of formula ratio,
Catalase, deoxyribonuclease, carrotene and acetylcholine are added to the mixed of medical stone and humic acid while stirring
It closes and is sufficiently stirred in object, make urase, catalase, deoxyribonuclease, carrotene and acetylcholine in humic acid
It is uniformly attached on medical stone under dilution, pulvis is then made or granule obtains the alkaline land soil microbial growth side
Method.
5. alkaline land soil microbial growth agent according to claim 4, it is characterised in that alkaline land soil obtained is micro-
Biological multiplication agent will seal, and prevent moisture from evaporating.
6. the preparation method of alkaline land soil microbial growth agent of any of claims 1-3, it is characterised in that packet
It includes and mixes in proportion humic acid and medical stone；By the urase of formula ratio, catalase, deoxyribonuclease, Hu
Radish element and acetylcholine, are added in the mixture of medical stone and humic acid while stirring and are sufficiently stirred, make urase, peroxidating
Hydrogen enzyme, deoxyribonuclease, carrotene and acetylcholine are uniformly attached on medical stone under the dilution of humic acid, then
Pulvis is made or granule obtains the alkaline land soil microbial growth method.
7. application of the alkaline land soil microbial growth agent of any of claims 1-5 in salt affected soil improvement.
8. a kind of method for promoting alkaline land soil microbial growth, it is characterised in that apply claim into alkaline land soil
Alkaline land soil microbial growth agent described in any one of 1-5.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110087141A (en) * | 2010-01-25 | 2011-08-02 | 주식회사 비지텍 | Method for improving soft ground using urease-producing microorganism |
| CN105891368A (en) * | 2016-05-10 | 2016-08-24 | 天津医科大学总医院 | Method for detecting acetyl choline content in peripheral blood mononuclear cells |
| CN108865166A (en) * | 2018-08-22 | 2018-11-23 | 江苏汇绿农业科技有限公司 | A kind of salt affected soil synergy modifying agent and its preparation method and application |
-
2019
- 2019-04-28 CN CN201910350145.7A patent/CN110184071A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110087141A (en) * | 2010-01-25 | 2011-08-02 | 주식회사 비지텍 | Method for improving soft ground using urease-producing microorganism |
| CN105891368A (en) * | 2016-05-10 | 2016-08-24 | 天津医科大学总医院 | Method for detecting acetyl choline content in peripheral blood mononuclear cells |
| CN108865166A (en) * | 2018-08-22 | 2018-11-23 | 江苏汇绿农业科技有限公司 | A kind of salt affected soil synergy modifying agent and its preparation method and application |
Non-Patent Citations (9)
| Title |
|---|
| LANSING M. PRESCOTT 等: "《微生物学》", 31 July 2003, 高等教育出版社 * |
| 丘钜世 等: "《麻风》", 31 October 1983, 上海科学技术出版社 * |
| 全国农业技术推广服务中心: "《中国有机肥料资源》", 30 April 1999, 中国农业出版社 * |
| 北京市果树产业协会 等: "《苹果有机栽培新技术》", 31 May 2007, 科学技术文献出版社 * |
| 王家玲 等: "《环境微生物学》", 31 January 2004, 高等教育出版社 * |
| 王敬国: "《生物地球化学—物质循环与土壤过程》", 31 March 2017, 中国农业大学出版社 * |
| 王正银: "《肥料研制与加工》", 31 December 2015, 中国农业大学出版社 * |
| 王白燕 等: "《病理生理学》", 30 November 2017, 延边大学出版社 * |
| 程炳嵩 等: "《植物生理与农业研究》", 31 August 1995, 中国农业科技出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760097A (en) * | 2019-11-06 | 2021-05-07 | 汪杨 | Saline-alkali soil conditioner and preparation method thereof |
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