CN110172053A - The extracting method of fisetin in a kind of fruits and vegetables - Google Patents
The extracting method of fisetin in a kind of fruits and vegetables Download PDFInfo
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- CN110172053A CN110172053A CN201910598005.1A CN201910598005A CN110172053A CN 110172053 A CN110172053 A CN 110172053A CN 201910598005 A CN201910598005 A CN 201910598005A CN 110172053 A CN110172053 A CN 110172053A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/30—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only not hydrogenated in the hetero ring, e.g. flavones
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides a kind of extracting method of fisetin in fruits and vegetables, the following steps are included: raw material is chosen, slice, pH value is adjusted the extracting method, filtering, refluxing extraction, ethanol reagent steam, dry, detection, in the fruits and vegetables fisetin extracting method design rationally, can it is efficient, quick and it is reproducible fisetin is extracted from fruits and vegetables, for exploitation fisetin potential therapeutic value technical method is provided.
Description
Technical field
The invention belongs to fisetin extractive technique field, in particular to the extracting method of fisetin in a kind of fruits and vegetables.
Background technique
Fisetin is because of the flavonoid drugs that most start to extract in Anacardiaceae plant, so it is also fisetin,
There are three phenolic hydroxyl groups in molecular structure, weakly acidic, also known as paint yellow acid.Molecular formula is C15H10O6, to be faint yellow, is dissolved in second
Alcohol, acetone etc. do not dissolve in petroleum ether at salt in alkaline water, and dirty-green can be observed after being added to Diluted Alcohol aqueous slkali in chloroform etc.
Fluorescence.
Pharmacological experiment study shows that fisetin is the natural products with multi-biological active function, the physiology of fisetin
Active function includes: 1. anti-oxidant: containing there are four hydroxyl in fisetin molecule, the ability with removing free radical can be prevented
The toxic action of oxidative damage and inhibition H2O2 to nerve cell to spongiocyte of NO.Fisetin can increase glutathione
Level improves the survival ability of cell.2. anti-inflammatory: fisetin can inhibit inflammatory cytokine to generate, by inhibition cell and carefully
Effect between born of the same parents, prevent mast cell from activating, also can be reduced the generation such as inflammatory factor and eosinophil.3. anti-swollen
Tumor: lot of documents report points out that fisetin all has a strong inhibitory effect melanoma and prostate cancer etc. at present.
It is exactly mainly to influence cell signalling, hinders cancer cell multiplication, cancer cell specific induction of apoptosis inhibits cancer cell migration and invasion etc.
Various ways reach therapeutic effect.4. treating Alzheimer disease (AD): the scientists discovery of Sa Ke biological study institute, the U.S.
In AD mouse model, the fisetin of low dosage can prevent the AD mouse progressive loss of memory and learning disorder.5. anti-aging:
Fisetin increases cellular glutathione content, mitochondrial function is maintained under oxidative stress status as a kind of antioxidant, right
Microglia has anti-inflammatory activity.After scientist is rich in the diet of fisetin to aged mouse feeding, the service life of mouse extends
About 10%.6. anticoagulation: fisetin can prevent and treat thrombotic diseases and the disease with hypercoagulative state, be a kind of very promising
Anticoagulation, antithrombotic drug.When the experimental study of external blood coagulation resisting function shows that fisetin can significantly extend full blood coagulation
Between, and can significantly mitigate molded thrombus weight and improve thrombus dissolution rate.7. immunological regulation: fisetin can inhibit T lymph
The expression of cell surface molecule CD69, CD25 inhibit the generation of the T lymphocyte proliferation and active oxygen of ConA induction, can also be with
The T lymphocyte apoptosis for promoting induced by dexamethasone, shows that fisetin is a kind of potential immunosuppressor.
Fruits and vegetables of the report containing fisetin have strawberry, persimmon, grape, Kiwi berry, peach, lotus rhizome, onion and cucumber at present,
Wherein concentration is up to 160 μ g/g in strawberry, and concentration is minimum in cucumber, greatly containing about 0.1 μ g/g.Fisetin is also largely present in respectively
In the tree and shrubs of kind pulse family class.At present about the research of fisetin and few is extracted from natural food materials, reported in the past
Extracting fisetin from the cured tree of Anacardiaceae plant wood is mainly solvent extraction method, be first with 60% to 70% ethanol water with
Dilute calcium hydroxide solution impregnates 24 hours, refilters rear filter residue using ethanol solution refluxing extraction one and a half hours, sums up and mention
Take optimised process are as follows: 90% ethanol solution flows back 3 hours, solid-to-liquid ratio 1:40, and temperature is 80 degrees Celsius, extraction rate
2.885%, and measure extract under ultraviolet specrophotometer and go out to have absorption in 245nm and 364nm, it is shown to be flavonol
The characteristic absorption of structure and flavonols structure, to prove that extract is flavonols.In addition, research is reported from lacquer tree
The optimised process of section smoke tree extraction fisetin: 65% ethanol water, solid-to-liquid ratio 1:25, solution ph 9, return time 90
Minute, refluxing extraction twice, recovery rate 1.03%.Verifying is repeatedly extracted, cannot all be contained from fisetin using above method
The fisetin for effectively extracting in highest strawberry and having absorption peak at 364nm is measured, illustrates that above method is not all suitable for from fruits and vegetables
Middle extraction fisetin.Therefore, a kind of method for probing into efficient, quick, reproducible extraction fisetin from fruits and vegetables very must
It wants, for this purpose, the present invention proposes a kind of extracting method of fisetin in fruits and vegetables.
Summary of the invention
Of the existing technology in order to solve the problems, such as, the present invention provides a kind of extracting methods of fisetin in fruits and vegetables, should
In fruits and vegetables fisetin extracting method design rationally, can it is efficient, quick and it is reproducible fisetin is extracted from fruits and vegetables,
Technical method is provided for the exploitation potential therapeutic value of fisetin.
To achieve the goals above, the present invention is to realize by the following technical solutions: fisetin in a kind of fruits and vegetables
Extracting method, the extracting method the following steps are included:
Step 1: raw material is chosen；Suitable fresh strawberry or other fruits and vegetables containing fisetin are chosen, are washed with water clean
After thinly slice to be placed in porcelain dish to be placed in constant temperature bellows and be dried, temperature setting is 60 DEG C, spare after dry；
Step 2: slice；Dry slice is polished into pulverizer spare after powder；
Step 3: pH value is adjusted；It weighs 10g strawberry powder to be placed in 500ml round-bottomed flask, be added by the material ratio of 1:20
Then 65% ethyl alcohol of 200ml is adjusted to 9-10, then the temperature strip with water-bath at 80 DEG C with the calcium hydroxide solution ph value of saturation
1.5h is heated to reflux under part；
Step 4: filtering；Extracting solution is filtered while hot, collects filtrate and filter residue is spare；
Step 5: refluxing extraction；Refluxing extraction is carried out to filter residue, is filtered after 1.5h, filtrate is collected, by this filtrate and step
Filtrate in four merges, and it is spare to obtain extracting solution；
Step 6: ethanol reagent steams；The ethanol reagent in extracting solution is steamed completely using Rotary Evaporators, setting temperature
Angle value is 40 DEG C, and revolving speed is suitable for recycling dehydrated alcohol；
Step 7: dry；Ethyl alcohol steam after extracting solution it is visible have yellow crystal, collect filter residue after filtering, be subsequently placed in
It is dried in 106 DEG C of thermostatic drying chamber, it is spare；
Step 8: detection；Obtained dry product is dissolved in dehydrated alcohol, is existed with ultraviolet specrophotometer measurement sample
The ultra-violet absorption spectrum of 200nm-460nm obtains its characteristic absorption.
As a kind of preferred embodiment of the invention, need to be dried to constant weight when slice being dried in the step 1 is
Only.
As a kind of preferred embodiment of the invention, the condition of filter residue reflux is the temperature condition at 80 DEG C in the step 5
It is lower to use water-bath refluxing extraction.
As a kind of preferred embodiment of the invention, filter residue need to be dried to constant weight in the step 7.
Beneficial effects of the present invention:
1, the extracting method design of fisetin rationally, can be efficient, quick and reproducible from fruits and vegetables in this fruits and vegetables
Middle extraction fisetin provides technical method for the exploitation potential therapeutic value of fisetin.
2, in this fruits and vegetables fisetin extracting method wide adaptability, can be effectively from the highest strawberry of fisetin content
Or other fruits and vegetables containing fisetin extract the fisetin for having absorption peak at 364nm, can effectively expand coming for fisetin
Source, and improve the efficiency of fisetin extraction.
3, the extracting method of fisetin uses solvent extraction method in this fruits and vegetables, and alkali carries acid is heavy, proposes a kind of completely new non-
The method of plucked instrument ketone can efficiently extract natural activity antioxidant fisetin in fruits and vegetables, and step is succinct, be suitble to promote.
Detailed description of the invention
Fig. 1 is a kind of step flow diagram of the extracting method of fisetin in fruits and vegetables；
Fig. 2 is the uv absorption spectra for extracting fisetin in a kind of fruits and vegetables in the strawberry of the extracting method of fisetin；
Fig. 3 is the uv absorption spectra for extracting fisetin in a kind of fruits and vegetables in the apple of the extracting method of fisetin；
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to
Specific embodiment, the present invention is further explained.
It please refers to Fig.1 to Fig.3, the present invention provides a kind of technical solution: the extracting method of fisetin in a kind of fruits and vegetables, it is described
Extracting method the following steps are included:
Step 1: raw material is chosen；Suitable fresh strawberry or other fruits and vegetables containing fisetin are chosen, are washed with water clean
After thinly slice to be placed in porcelain dish to be placed in constant temperature bellows and be dried, temperature setting is 60 DEG C, spare after dry；
Step 2: slice；Dry slice is polished into pulverizer spare after powder；
Step 3: pH value is adjusted；It weighs 10g strawberry powder to be placed in 500ml round-bottomed flask, be added by the material ratio of 1:20
Then 65% ethyl alcohol of 200ml is adjusted to 9-10, then the temperature strip with water-bath at 80 DEG C with the calcium hydroxide solution ph value of saturation
1.5h is heated to reflux under part；
Step 4: filtering；Extracting solution is filtered while hot, collects filtrate and filter residue is spare；
Step 5: refluxing extraction；Refluxing extraction is carried out to filter residue, is filtered after 1.5h, filtrate is collected, by this filtrate and step
Filtrate in four merges, and it is spare to obtain extracting solution；
Step 6: ethanol reagent steams；The ethanol reagent in extracting solution is steamed completely using Rotary Evaporators, setting temperature
Angle value is 40 DEG C, and revolving speed is suitable for recycling dehydrated alcohol；
Step 7: dry；Ethyl alcohol steam after extracting solution it is visible have yellow crystal, collect filter residue after filtering, be subsequently placed in
It is dried in 106 DEG C of thermostatic drying chamber, it is spare；
Step 8: detection；Obtained dry product is dissolved in dehydrated alcohol, is existed with ultraviolet specrophotometer measurement sample
The ultra-violet absorption spectrum of 200nm-460nm obtains its characteristic absorption.
As a kind of preferred embodiment of the invention, need to be dried to constant weight when slice being dried in the step 1 is
Only.
As a kind of preferred embodiment of the invention, the condition of filter residue reflux is the temperature condition at 80 DEG C in the step 5
It is lower to use water-bath refluxing extraction.
As a kind of preferred embodiment of the invention, filter residue need to be dried to constant weight in the step 7.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention, for this field skill
For art personnel, it is clear that invention is not limited to the details of the above exemplary embodiments, and without departing substantially from spirit of the invention or
In the case where essential characteristic, the present invention can be realized in other specific forms.Therefore, in all respects, should all incite somebody to action
Embodiment regards exemplary as, and is non-limiting, the scope of the present invention by appended claims rather than on state
Bright restriction, it is intended that including all changes that fall within the meaning and scope of the equivalent elements of the claims in the present invention
It is interior.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (4)
1. the extracting method of fisetin in a kind of fruits and vegetables, which is characterized in that the extracting method the following steps are included:
Step 1: raw material is chosen；Suitable fresh strawberry or other fruits and vegetables containing fisetin are chosen, are cut after being washed with water completely
Flakiness is placed in porcelain dish to be placed in constant temperature bellows and be dried, and temperature setting is 60 DEG C, spare after dry；
Step 2: slice；Dry slice is polished into pulverizer spare after powder；
Step 3: pH value is adjusted；It weighs 10g strawberry powder to be placed in 500ml round-bottomed flask, 200ml is added by the material ratio of 1:20
65% ethyl alcohol, then 9-10 is adjusted to the calcium hydroxide solution ph value of saturation, then with water-bath under the conditions of 80 DEG C of temperature
It is heated to reflux 1.5h；
Step 4: filtering；Extracting solution is filtered while hot, collects filtrate and filter residue is spare；
Step 5: refluxing extraction；Refluxing extraction is carried out to filter residue, is filtered after 1.5h, collects filtrate, it will be in this filtrate and step 4
Filtrate merge, it is spare to obtain extracting solution；
Step 6: ethanol reagent steams；The ethanol reagent in extracting solution is steamed completely using Rotary Evaporators, set temperature value
It is 40 DEG C, revolving speed is suitable for recycling dehydrated alcohol；
Step 7: dry；Ethyl alcohol steam after extracting solution it is visible have yellow crystal, collect filter residue after filtering, be subsequently placed in 106 DEG C
Thermostatic drying chamber in be dried, it is spare；
Step 8: detection；Obtained dry product is dissolved in dehydrated alcohol, with ultraviolet specrophotometer measurement sample in 200nm-
The ultra-violet absorption spectrum of 460nm obtains its characteristic absorption.
2. the extracting method of fisetin in a kind of fruits and vegetables according to claim 1, it is characterised in that: right in the step 1
Until slice need to be dried to constant weight when being dried.
3. the extracting method of fisetin in a kind of fruits and vegetables according to claim 1, it is characterised in that: filtered in the step 5
The condition of slag reflux is water-bath refluxing extraction under the conditions of 80 DEG C of temperature.
4. the extracting method of fisetin in a kind of fruits and vegetables according to claim 1, it is characterised in that: filtered in the step 7
Slag need to be dried to constant weight.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102924419A (en) * | 2012-10-26 | 2013-02-13 | 西安宏冠生物科技有限公司 | Method for extracting fisetin from boxwood |
| US20170252319A1 (en) * | 2016-03-04 | 2017-09-07 | Korea Advanced Institute Of Science And Technology | Flavonoid-Poly(ethylene glycol) Complex Comprising Flavonoid Compounds and Poly(ethylene glycol) and Preparation Method Thereof |
| CN108003125A (en) * | 2017-11-30 | 2018-05-08 | 天峨县平昌生态农业有限公司 | A kind of method that fisetin is extracted from wild paint |
| CN109369589A (en) * | 2018-10-12 | 2019-02-22 | 王刚 | The synchronization extraction process of smoke tree flavonoid glycoside and its application |
-
2019
- 2019-07-04 CN CN201910598005.1A patent/CN110172053A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102924419A (en) * | 2012-10-26 | 2013-02-13 | 西安宏冠生物科技有限公司 | Method for extracting fisetin from boxwood |
| US20170252319A1 (en) * | 2016-03-04 | 2017-09-07 | Korea Advanced Institute Of Science And Technology | Flavonoid-Poly(ethylene glycol) Complex Comprising Flavonoid Compounds and Poly(ethylene glycol) and Preparation Method Thereof |
| CN108003125A (en) * | 2017-11-30 | 2018-05-08 | 天峨县平昌生态农业有限公司 | A kind of method that fisetin is extracted from wild paint |
| CN109369589A (en) * | 2018-10-12 | 2019-02-22 | 王刚 | The synchronization extraction process of smoke tree flavonoid glycoside and its application |
Non-Patent Citations (2)
| Title |
|---|
| 李建华 等: "黄栌有效成分漆黄素提取工艺研究", 《中华中医药杂志(原中国医药学报)》 * |
| 禾本: "草莓可抑制阿尔茨海默病", 《中国果业信息》 * |
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Effective date of registration: 20191012 Address after: 435000 No. 16 Guilin North Road, Hubei, Huangshi Applicant after: HUBEI POLYTECHNIC University Address before: Room 508, Unit 4, Building 3, Room 32, Guilin South Road, Xiashu District, Huangshi City, Hubei Province Applicant before: Ma Hongying |
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Application publication date: 20190827 |