CN108815036A - A kind of preparation method and applications of Ta Naka extract - Google Patents

A kind of preparation method and applications of Ta Naka extract Download PDF

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Publication number
CN108815036A
CN108815036A CN201810861270.XA CN201810861270A CN108815036A CN 108815036 A CN108815036 A CN 108815036A CN 201810861270 A CN201810861270 A CN 201810861270A CN 108815036 A CN108815036 A CN 108815036A
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extract
naka
microwave
pretreatment
skin
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不公告发明人
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Guangdong Chuangmei Anti-aging Research Co., Ltd.
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Foshan Qianru Cosmetics Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/18—Antioxidants, e.g. antiradicals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/81—Preparation or application process involves irradiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying

Abstract

The invention discloses the preparation method of Ta Naka extract and its applications in cosmetics.The Ta Naka extract is the extract of Ta Naka bark, which has good DPPH free radical scavenging ability, be good natural, have the functions such as outstanding anti-aging, whitening, be good natural plants cosmetic material.

Description

A kind of preparation method and applications of Ta Naka extract
Technical field
The present invention relates to cosmetic fields, more particularly to a kind of preparation method and applications of Ta Naka extract.
Background technique
Tower, which is received, to be stuck in Burma with long usage history and is widely applied crowd, is the important symbol of Burma's culture. Walking is in any streets and lanes of Burma, school or airport etc., can encounter and some smear with punctuation marks on the face The people of pale yellow powder, it is in the majority with women and children.This pale yellow powder is a kind of tradition shield for being commonly called as pigment or incense wood powder Skin product.It is referred to as " Thanakha in Burma（Or Thanaka）", transliteration is " Ta Naka ".Ta Naka, and be commonly referred to as " Theethee " or " wood apple ", is the stem skin or root skin of rutaceae, and current received Latin is entitledHesperethusa crenulata (Roxb.) M. Roem., different name areLimonia crenulata Roxb, also there is it Its data mentions different nameNaringi crenulata、Limonia acidissima Groff（Wangthong et al., 2010）Or yellow fragrant chinaberry（Jiang Shan and Sun Peidong, 2014）.
Dermatology magazine ran is investigated according to the U.S.（Seiverling & Ahrns, 2013）, tower receives that be stuck in Burma civil Approved skin-protecting function has sun-proof, anti-acne, and whitening is refrigerant, adorns, antipruritic, scar of dispelling, and reduces wrinkle and inhibits peculiar smell totally nine Kind.Wherein, sun-proof is important preparation before the people's outdoor activities of torrid areas, and Ta Naka is the real of oneself civil exploitation Pure natural sun-proof articles, studies have shown that production Ta Naka Hesperethusa crenulata bark contain it is different it is pale reddish brown before Hu lactone（Marmesin）, have the function of ultraviolet radiation absorption, ultraviolet light can be effectively filtered out（Joo et al., 2004）.This Outside, Ta Naka is also possible to that there is some other to wait to be studied well-verified skin care effect.For example, tower, which is received, is stuck in torrid areas There are important potentiality in terms of mosquito repellent.
Studies have shown that the mosquito-proof effect of stem skin powder itself is although very limited, and effective time is few for wood apple It, can effectively mosquito-proof 10 hours but if it is used in mixed way with Metadelphene in 3 hours.Infer accordingly, wood apple stem skin powder When mixed with Metadelphene, " sustained release agent " of Metadelphene effective component may be acted as（Lindsay et al., 1998）.Thailand Scholar Wangthong is equal to 2010 in ethnic drug magazine《Journal of Ethnopharmacology》On for the first time be directed to tower Receive card tradition using reportingHesperethusa crenulataThe bioactivity and safety research result of stem skin.Experiment Prove that it is living with strong anti-inflammatory activity and significant antioxidant activity and slight tyrosinase inhibitory activity and antibacterium Property, which will not cause negative effect to general application without genetoxic, slight cytotoxicity.In cosmetics In there is great potential application value.
Summary of the invention
The purpose of the present invention is to provide Ta Naka extracts, using it as the cosmetics of active constituent, its preparation method, And its preparing the application in cosmetics.The present invention specifies that Ta Naka extract has good skin effect and safety, Become good natural skin care cosmetic raw materials.
In order to realize above-mentioned purpose of the invention, the present invention provides the following technical solutions：
A kind of Ta Naka extract, is prepared by the following method and obtains：
S1. vacuum microwave pre-processes：Ta Naka bark is taken, is crushed, sieving is transferred in vacuum microwave, carries out vacuum microwave Pretreatment, obtains pretreatment product;
S2. Extraction solvent is added to step S1 to be made in pretreatment product, refluxing extraction collects extracting solution, and concentrate drying is Get Ta Naka extract.
In wherein some embodiments, crushing described in the step S1 is grinding or shearing or Ultrasonic Pulverization.
In wherein some embodiments, sieving described in the step S1 is the sieving of 60-80 mesh.
In wherein some embodiments, vacuum microwave pretreatment vacuum degree is 0.07-0.08MPa in the step S1, micro- Wave handles the time as 6-8min.
In wherein some embodiments, vacuum microwave is pre-processed in the step S1, and maintenance cavity temperature is 160-180 DEG C, microwave treatment dosage is 1.8-2.5kw/kg, preferably 2 kw/kg.
In wherein some embodiments, the weight ratio of Extraction solvent described in the step S2 and pretreatment product is（10- 30）：1；The Extraction solvent is the ethanol solution of volume fraction 40-60% or the acetone soln of 40-60%, preferably volume point The ethanol solution of number 60%.
In wherein some embodiments, it is every time 3- that the number of refluxing extraction described in the step S2, which is 2-4 times, 4h is carried out, preferably 55 DEG C at 50-60 DEG C.
In wherein some embodiments, the step S2 can also make further the extracting solution after obtaining extracting solution Purification, for example, by using the methods of ion exchange resin, activated-charcoal column or film filtering, preferably film is filtered.
Wherein, the film filtering, for less than or equal to 8000 dalton micro-filtrate membrane filtrations, more preferably less than or equal to 5000 dongles The micro-filtrate membrane filtration to pause.
It, can also be further pure to the extract after the step S2 obtains extract in wherein some embodiments Change：The purifying purifies, with anhydrous second for 1 part of extract is dissolved into aqueous solution with 100 parts of water through macroporous resin adsorption Alcohol elution, concentrate eluant are evaporated get Ta Naka extract.
Inventors be surprised to learn that vacuum microwave processing has larger impact to the efficiency of subsequent extracted, at vacuum microwave After reason, extraction efficiency is high, destroys less to the flavone compound of Ta Naka, especially has to the yield of the flavone compound of acquisition Larger impact.Do not limited by particular theory, thus it is speculated that itself the possible reason is handled by vacuum microwave, due to microwave frequency with The whirling vibration frequency of chemical group is close, can be used to change the conformation of molecule, selectively activates certain reactive groups, promotees Theory of evolution reaction, inhibits side reaction, or be the moisture fast vaporizing in cell, is formed and acted on to the vapour pressure of external diffusion Cell wall, and make breakthroughs in weak links such as pit membranes, cause pit membrane to rupture, to be conducive to subsequent extracted.
The present invention also provides Ta Naka extracts to prepare the application in cosmetics.
Further, the Ta Naka extract is preparing the application in tender skin or anti-aging or skin-lightening cosmetic.
Further, application of the Ta Naka extract as cosmetic additive agent in cosmetics, additive amount account for makeup The 0.01-1wt% of product gross mass.
Further, the cosmetics are facial treatment milk.
The present invention also provides a kind of facial treatment milk, the component including following mass percentage：
Glycerol caprylate 0.1-0.5%,
Glycerol three（Thylhexoic acid）Ester 0.1-2%,
Stearoyl dilactic acid sodium 0.1-1%,
Natrulon H-10 myristinate 0.1-1%,
Hexylene glycol 1-3%,
Glycerol 3-8%,
Citric acid 0.01-0.1%,
Carbohydrate isomer 0.1-1%,
Sodium citrate 0.01-0.1%,
Carbomer 0.1-0.5%,
PEG-40 rilanit special 0.01-0.05%,
Aminomethyl propanol 0.1-0.5%,
Beta glucan 0.1-3%,
EDTA disodium 0.01-0.05%,
Rose（ROSA RUGOSA）Caul-fat 0.0001-0.001%,
Tapioca 0.1-0.5%,
Sodium Hyaluronate 0.01-0.05%,
Parahydroxyacet-ophenone 0.2-0.4%,
Above-mentioned Ta Naka extract 0.01-1%,
Water surplus.
Compared with prior art, the present invention has following beneficial effect：
The present invention by tower receive card carry out concentration extraction obtain Objective extraction object, to Objective extraction object carried out cytotoxicity reality It tests and cosmetics for skin activity experiment, it was demonstrated that the safety of Objective extraction object specifies the excellent cosmetics of Objective extraction object Activity and pharmaceutical activity.The present invention improves the utilization function and added value of Ta Naka, and it is former to have expanded natural skin care product plant The source of material.The present invention provides a kind of Ta Naka extracting methods, including：After Ta Naka powder is carried out vacuum microwave pretreatment It extracts, obtains extract, the extracting method extraction efficiency is high, destroys few, the extracting method to the flavone compound of Ta Naka The Ta Naka extractive total flavone content of extraction is high, facilitates the performance of Ta Naka effect, also, the extracting method is easy to grasp Make, is suitable for industrialization promotion application.The results show, the Ta Naka extract high safety which extracts, can A variety of free radicals are removed, have significant ultra-violet radiation resisting effect and whitening and softening skin, increases skin elasticity, reduces wrinkle Effect.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention will be made into one below Step ground detailed description, embodiments of the present invention are not limited thereto, for not specifically specified technological parameter or condition, can refer to Routine techniques carries out.
Embodiment 1
The preparation of Ta Naka extract：
S1. Ta Naka bark 100g, grind into powder are weighed, the sieving of 60 mesh is transferred in vacuum microwave, carries out vacuum microwave Pretreatment, holding vacuum degree are 0.07MPa, microwave dose 1.8kw/kg, handle time 6min, maintenance microwave cavity temperature is 180 DEG C, obtain pretreatment product；
S2. after the pretreatment of S1 step vacuum microwave, the ethanol solution that the volume fraction measured with 10 times is 60% is added to pre- place It manages in product, in 50 DEG C of refluxing extraction 3h, is repeated 2 times, collect extracting solution, concentration, 50 DEG C of dryings, get Ta Naka extract.
Embodiment 2
The preparation of Ta Naka extract：
S1. Ta Naka bark 100g, grind into powder are weighed, the sieving of 80 mesh is transferred in vacuum microwave, carries out vacuum microwave Pretreatment, holding vacuum degree are 0.08MPa, microwave dose 2.5kw/kg, handle time 8min, maintenance microwave cavity temperature is 160 DEG C, obtain pretreatment product；
S2. after the pretreatment of S1 step vacuum microwave, the acetone soln that the volume fraction measured with 30 times is 40% is added to pre- place It manages in product, in 60 DEG C of refluxing extraction 4h, is repeated 4 times, collecting extracting solution must be refined using 8000 dalton micro-filtrate membrane filtrations Purification filtrate is evaporated under reduced pressure with Rotary Evaporators, is concentrated, 60 DEG C of dryings obtain crude extract, with 100 parts of water 1 part by filtrate Extract is dissolved into aqueous solution, through macroreticular resin (D101) adsorption and purification, then with anhydrous ethanol elution, collects eluent concentration, Frozen drying, get Ta Naka extract.
Embodiment 3
The preparation of Ta Naka extract：
S1. Ta Naka bark 100g, grind into powder are weighed, the sieving of 70 mesh is transferred in vacuum microwave, carries out vacuum microwave Pretreatment, holding vacuum degree are 0.075MPa, microwave dose 2kw/kg, handle time 7min, maintenance microwave cavity temperature is 170 DEG C, obtain pretreatment product；
S2. after the pretreatment of S1 step vacuum microwave, the ethanol solution that the volume fraction measured with 20 times is 50% is added to pre- place It manages in product, in 55 DEG C of refluxing extraction 3.5h, is repeated 3 times, collect extracting solution and obtained smart using 5000 dalton micro-filtrate membrane filtrations Purification filtrate is evaporated under reduced pressure with Rotary Evaporators, is concentrated, 60 DEG C of dryings obtain crude extract, with 100 parts of water 1 part by filtrate processed Extract be dissolved into aqueous solution, through macroreticular resin (D101) adsorption and purification, then with anhydrous ethanol elution, it is dense to collect eluent Contracting, frozen drying, get Ta Naka extract.
Comparative example 1
Without vacuum microwave processing step, remaining step and condition are the same as embodiment 1.
Comparative example 2
Without vacuum microwave processing step, remaining step and condition are the same as embodiment 2.
Comparative example 3
Without vacuum microwave processing step, remaining step and condition are the same as embodiment 3.
Test 1, general flavone content detection
1) preparation of reference substance solution
Precision weighs control substance of Rutin 5mg, sets in 25mL brown volumetric flask, is diluted to scale after being dissolved with a small amount of methanol solution, Obtain the rutin contrast solution that concentration is 0.2mg/mL；
2) the preparation precision of standard curve measures control substance of Rutin solution 0mL, 1mL, 2mL, 3mL, 4mL, 5mL, 6mL, sets respectively In 25mL volumetric flask, 6 .0mL are respectively added water to, add 5% sodium nitrite solution 1mL, are mixed, add 1% hydroxide after placing 6min Sodium test solution 10mL, finally adds water to scale, shakes up, and places 15min.Absorbance value is measured at 510nm, is vertical with absorbance value Coordinate, reference substance concentration (mg/mL) are abscissa, draw standard curve, and find out normal equation；
3) sample measures
The accurate Ta Naka extract for measuring 1.0mL embodiment 1-6 (should be appropriate dilute if the flavonoid concentration height in sample respectively Release and take 1.0mL for chromogenic assay again), it sets in 25mL volumetric flask, according to the method under " preparation of standard curve " item, " adds certainly Water rises to 6.0mL ", measures absorbance value in accordance with the law, from the concentration (mg/ for reading general flavone in test solution on standard curve ML), reconvert is after being calculated by calculation formula up to general flavone content in sample, and the results are shown in Table 3, calculation formula：
X=C × V2/V1
X is general flavone content in sample, unit mg/mL
C is from the concentration for checking in general flavone in test solution on standard curve, unit mg/mL；
V1Volume, unit mL are measured for sample；
V2For sample constant volume, unit mL.
General flavone content in the different embodiment Ta Naka extracts of table 3
As known from Table 3, the general flavone content of the resulting Ta Naka extract of 1-3 of the embodiment of the present invention is apparently higher than comparative example 1- 3.It can be seen that the content of general flavone is higher in the Ta Naka extract that the present invention obtains after vacuum microwave pre-processes, very Empty Microwave Pretreatment is committed step, the step for have larger impact to the yield of general flavone.
Test the cytotoxicity experiment of 2 Ta Naka extracts
By MTS colorimetric method, the CC of sample is determined50(50%cytotoxicconcentration) produces 50% cell Drug concentration when toxigenicity, so that it is determined that the secure sample concentration of following activity experiment.
Experimental method：In 96 porocyte culture plates, B16 cell is mixed with the drug solution to be measured of various concentration, setting 3 A repeating hole, while the blank control of not drug containing is set, 37 DEG C, 5%CO2It cultivates for 24 hours, using MTS colorimetric determination cell Toxicity, microplate reader measure OD value, measure wavelength 490nm.CC is calculated50Value.
Cell survival rate (%)=experimental port OD490nm/ blank well OD490nm× 100%
Experimental result：The cell survival rate of Ta Naka extract obtained by embodiment 1-3 is respectively 87.34%, 88.55%, 87.16%, it is all larger than >=60% normal level, it is evident that Ta Naka extract does not have toxic action to cell, as skin The safety of the natural material of cosmetics is guaranteed.
Test 3, DPPH radicals scavenging experiment
By drug Ta Naka extract to be measured and DPPH (final concentration of 100 μM) hybrid reaction, 3 repeating holes are set, are set simultaneously The not blank control of drug containing and Trolox positive control are set, 30 DEG C, 1h, microplate reader measures OD value, Detection wavelength 515nm. Antioxygen rate is calculated.
Antioxygen rate (%)=(1- experimental port OD515nm/ blank well OD515nm) × 100%
Experimental result：In an experiment, Ta Naka extract shows significant DPPH free radical scavenging ability, and according to concentration When gradient 0-20mg/ml, good dose-effect relationship is presented, and when Ta Naka extract >=20mg/ml, embodiment 1-3 is resulting Ta Naka extract antioxygen rate can all reach >=92%, be used as having tender skin, anti-aging potentiality cosmetic active it is good Natural botanical source.
Embodiment 4：It is successively added to the Ta Naka extract of embodiment 1-3 and comparative example 1-3 in following formulas, makes At facial treatment milk, it is successively calculated as facial treatment milk 1-6, wherein embodiment 1-3 corresponds to facial treatment milk 1-3, and comparative example 1-3 corresponds to Facial treatment milk 4-6；
The facial treatment milk 1-6 includes each component of following mass percentage, as shown in table 1-2：
Table 1
Table 2
Efficacy test of the present invention：
4, crowd's test on probation
For the practical effect of test product, crowd's investigation on probation, group have been carried out respectively to sample made from facial treatment milk 1-6 Sample is sent out to 300 age 28-40 years old volunteers（Wherein women 218）, there is obscure relaxation of color spot, skin etc. in face Problem, volunteer are divided into 6 groups at random.After volunteer cleans facial skin, dries, appropriate facial treatment milk is applied to a left side with hand Side face portion, right side face does not smear, and as test comparison area, tested period face does not smear other cosmetics or drug, even It is continuous to check using effect using 30 days, and fill in questionnaire.
It is classified the standard determined：
It is significant effective：Opposite right side face, test block color spot problem are completely resolved, and skin smooth is for example new, the white and moist pool of jade-like stone, skin Skin high resilience.
Effectively：Opposite right side face, test block color spot is obviously thin out, forms color spot soon or even can completely disappear, skin Skin becomes pale, and skin becomes flexible.
In vain：Opposite right side face, test block color spot do not change without significant change or intensification, the colour of skin.
4 crowd of table tries out investigation result
Conclusion：In the facial treatment milk sample of the extract containing Ta Naka, it is added to the tower obtained after vacuum microwave pre-processes Receive the facial treatment milk 1-3 of card extract, after being used 30 days, skin smooth is for example new, and the white and moist pool of jade-like stone, skin becomes flexible.And The facial treatment milk 4-6 of the Ta Naka extract without obtaining after vacuum microwave pretreatment is added, after being used 30 days, skin improves Situation（Significant effective percentage≤9）It can not show a candle to the effect of facial treatment milk 1-3（Significant effective percentage >=41）.Illustrate to be added to this hair The facial treatment milk of bright obtained Ta Naka extract has the effect of good nti-freckle, tender skin and enhancing skin elasticity.
It is learnt through questionnaire, makes have facial treatment milk product of the present invention, mild non-stimulated, skin sense is good.
Embodiment described above only describe the preferred embodiments of the invention, not to model of the invention It encloses and is defined, without departing from the spirit of the design of the present invention, those of ordinary skill in the art are to technical side of the invention The various changes and improvements that case is made should all be fallen into the protection scope that claims of the present invention determines.

Claims (10)

1. a kind of extracting method of Ta Naka extract, which is characterized in that include the following steps：
S1. vacuum microwave pre-processes：Ta Naka bark is taken, is crushed, sieving is transferred in vacuum microwave, carries out vacuum microwave Pretreatment, obtains pretreatment product;
S2. Extraction solvent is added to step S1 to be made in pretreatment product, refluxing extraction collects extracting solution, and concentrate drying is Get Ta Naka extract.
2. extracting method according to claim 1, which is characterized in that crushing described in the step S1 is grinding or cuts It cuts or Ultrasonic Pulverization；The sieving is the sieving of 60-80 mesh；The vacuum microwave pretreatment vacuum degree is 0.07-0.08MPa, Microwave treatment time is 6-8min, and maintaining microwave cavity temperature is 160-180 DEG C, and microwave treatment dosage is 1.8-2.5kw/kg.
3. extracting method according to claim 1, which is characterized in that Extraction solvent described in the step S2 and pretreatment The weight ratio of product is（10-30）：1；The third of ethanol solution or 40-60% that the Extraction solvent is volume fraction 40-60% Ketone solution；The number of the refluxing extraction is 2-4 times, is every time 3-4h, is carried out at 50-60 DEG C.
4. extracting method according to claim 1, which is characterized in that the step S2 further includes after obtaining extracting solution Further purification is done to the extracting solution, the refining methd is using in the filtering of ion exchange resin, activated-charcoal column or film It is a kind of；Wherein, the film filtering, for less than or equal to 8000 dalton micro-filtrate membrane filtrations or less than or equal to the micro-filtration of 5000 dalton Film filtering；It further include that the extract is further purified after the step S2 obtains extract：The purifying is with 100 part Water 1 part of extract is dissolved into aqueous solution, purified through macroporous resin adsorption, with anhydrous ethanol elution, concentrate eluant steams Dry get Ta Naka extract.
5. the Ta Naka extract extracted by the described in any item extracting methods of claim 1-4.
6. Ta Naka extract according to claim 5 is preparing the application in tender skin or anti-aging or skin-lightening cosmetic.
7. application according to claim 6, which is characterized in that Ta Naka extract is as cosmetic additive agent in cosmetics In application.
8. application according to claim 7, which is characterized in that the Ta Naka extract additive amount accounts for cosmetics gross mass 0.01-1wt%.
9. application according to claim 8, which is characterized in that the cosmetics are facial treatment milk.
10. application according to claim 9, which is characterized in that the facial treatment milk includes following mass percentage Component：
Glycerol caprylate 0.1-0.5%,
Glycerol three（Thylhexoic acid）Ester 0.1-2%,
Stearoyl dilactic acid sodium 0.1-1%,
Natrulon H-10 myristinate 0.1-1%,
Hexylene glycol 1-3%,
Glycerol 3-8%,
Citric acid 0.01-0.1%,
Carbohydrate isomer 0.1-1%,
Sodium citrate 0.01-0.1%,
Carbomer 0.1-0.5%,
PEG-40 rilanit special 0.01-0.05%,
Aminomethyl propanol 0.1-0.5%,
Beta glucan 0.1-3%,
EDTA disodium 0.01-0.05%,
Rose（ROSA RUGOSA）Caul-fat 0.0001-0.001%,
Tapioca 0.1-0.5%,
Sodium Hyaluronate 0.01-0.05%,
Parahydroxyacet-ophenone 0.2-0.4%,
Ta Naka extract 0.01-1% as claimed in claim 5,
Water surplus.
CN201810861270.XA 2018-07-29 2018-07-29 A kind of preparation method and applications of Ta Naka extract Pending CN108815036A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109602675A (en) * 2019-01-17 2019-04-12 完美（中国）有限公司 A kind of composite vegetables extractive tyrosinase inhibitor and its application
CN110812291A (en) * 2018-08-07 2020-02-21 大江生医股份有限公司 Use of extract of Ternacard bark for preventing skin aging
CN114469768A (en) * 2022-03-17 2022-05-13 深圳市珍妮肤化妆品有限公司 Whitening composition and application thereof
CN114617816A (en) * 2022-04-13 2022-06-14 新柏妆(广州)科技研发中心有限公司 Anti-aging composition and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110812291A (en) * 2018-08-07 2020-02-21 大江生医股份有限公司 Use of extract of Ternacard bark for preventing skin aging
CN109602675A (en) * 2019-01-17 2019-04-12 完美（中国）有限公司 A kind of composite vegetables extractive tyrosinase inhibitor and its application
CN114469768A (en) * 2022-03-17 2022-05-13 深圳市珍妮肤化妆品有限公司 Whitening composition and application thereof
CN114469768B (en) * 2022-03-17 2023-08-25 深圳市珍妮肤化妆品有限公司 Whitening composition and application thereof
CN114617816A (en) * 2022-04-13 2022-06-14 新柏妆(广州)科技研发中心有限公司 Anti-aging composition and preparation method thereof
CN114617816B (en) * 2022-04-13 2023-10-03 新柏妆(广州)科技研发中心有限公司 Anti-aging composition and preparation method thereof

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