CN110161107A - A kind of electrophoresis tank convenient for fixed plastic plate - Google Patents

A kind of electrophoresis tank convenient for fixed plastic plate Download PDF

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Publication number
CN110161107A
CN110161107A CN201810292970.1A CN201810292970A CN110161107A CN 110161107 A CN110161107 A CN 110161107A CN 201810292970 A CN201810292970 A CN 201810292970A CN 110161107 A CN110161107 A CN 110161107A
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CN
China
Prior art keywords
plastic plate
electrophoresis tank
box body
sliding rail
convenient
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810292970.1A
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Chinese (zh)
Inventor
向丹
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangan Vocational and Technical College
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Guangan Vocational and Technical College
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Filing date
Publication date
Application filed by Guangan Vocational and Technical College filed Critical Guangan Vocational and Technical College
Priority to CN201810292970.1A priority Critical patent/CN110161107A/en
Publication of CN110161107A publication Critical patent/CN110161107A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories

Abstract

The invention discloses a kind of electrophoresis tanks convenient for fixed plastic plate, including electrophoresis tank and plastic plate, electrophoresis tank include groove body and lid, groove body both ends are equipped with electrode, the conducting ring being electrically connected to a power source is correspondingly provided on lid, when cover cap is together on groove body, electrode is connected with power supply;The inner wall of the side of groove body is equipped with the first sliding rail, and the upper end of the first sliding rail extends at the top of groove body, the sliding block of protrusion is correspondingly provided on the side of plastic plate, when sliding block from top to bottom slides into the first sliding rail, plastic plate is parallel with groove body.Plastic plate is fixed in groove body by a kind of electrophoresis tank convenient for fixed plastic plate of the present invention, ensure that the linearity of electrophoretic band, the case where will not intersecting between adjacent ribbons.

Description

A kind of electrophoresis tank convenient for fixed plastic plate
Technical field
The present invention relates to electrophoresis equipment technical field more particularly to a kind of electrophoresis tanks convenient for fixed plastic plate.
Background technique
Electrophoresis tank is the core component of gel electrophoresis system, including disc electrophoresis slot, vertical slab electrophoresis groove and level Electrophoresis tank three categories, wherein Horizontal electrophoresis tank is most widely used in nucleic acid separation qualification process.Horizontal electrophoresis tank includes electricity Swimming slot pedestal and electrode, wherein electrode connect the electric field to be formed from cathode to anode with external power supply, and nucleic acid or albumen are in In electric field, since itself is electrically charged so that nucleic acid or protein molecular towards the swimming of opposite polarity electrode one end also cross Gel layer realizes nucleic acid molecules separation, the layering of different molecular weight by means of the layering of gel layer, molecule inhibition, as a result, Form different bands.
At present, it is common to use Horizontal electrophoresis tank by the way that directly plastic plate (including gel) is placed in Horizontal electrophoresis tank Electrophoresis is carried out, still, as ion electrically charged in electrophoretic buffer to two end electrodes movement and generates gas in electrophoresis process Bubble, causes electrophoretic buffer to occur the flowing recycled and turbulent flow in Horizontal electrophoresis tank, to drive plastic plate in horizontal strip electrophoresis It is moved in slot, easily causes plastic plate azimuth shift, and then electrophoretic band is caused to deviate the sample phase between swimming lane or swimming lane Mutually obscure, seriously affects the effect of electrophoresis.
Summary of the invention
For overcome the deficiencies in the prior art, the purpose of the present invention is to provide a kind of electrophoresis convenient for fixed plastic plate Slot can be good at fixing plastic plate by the electrophoresis tank, prevent plastic plate movement or rotation, Jin Erke in electrophoresis process It takes and is easy to cause electrophoretic band to deviate swimming lane or occur between each other because of mobile or rotation in existing electrophoresis tank electrophoresis process The problem of obscuring.
The purpose of the present invention adopts the following technical scheme that realization:
A kind of electrophoresis tank convenient for fixed plastic plate, including electrophoresis tank and plastic plate, the electrophoresis tank include groove body and lid Body, the groove body both ends are equipped with electrode, are correspondingly provided with the conducting ring being electrically connected to a power source on the lid, when the cover cap closes When on groove body, the electrode is connected with power supply;
The inner wall of the side of the groove body is equipped with the first sliding rail, and the upper end of first sliding rail extends at the top of groove body, The sliding block of protrusion is correspondingly provided on the side of the plastic plate, when sliding block from top to bottom slides into the first sliding rail, the plastic plate It is parallel with groove body.
Further, first sliding rail is arranged in vertical direction.
Further, the length of first sliding rail is 15~25mm.
Further, the groove body bottom surface forms the horizontal positioned platform for placing plastic plate, institute to groove body inner recess It states the first sliding rail lower end and extends to horizontal positioned platform.
Further, first sliding rail includes a pair, a pair of first sliding rail to being set on the two sides of horizontal positioned platform, Correspondingly, sliding block is on the two sides of plastic plate.
Further, the electrophoresis tank convenient for fixed plastic plate further includes the cryogenic box for accommodating electrophoresis tank, described Cryogenic box includes upper layer box body and lower layer box body, and horizontal grate is equipped between the upper layer box body and lower layer box body;
Drawer is equipped in the lower layer box body, when the drawer is relative to upper layer bin movement, drawer is selectively contained in In lower layer box body or pull out outside lower layer box body.
Further, the lower layer box body is equipped with horizontal slide rail, any of the horizontal slide rail perpendicular to lower layer box body Side;
The drawer bottom is equipped with idler wheel, and the idler wheel is rolled along horizontal slide rail.
Further, it is additionally provided with handle on the outer surface of the drawer, when drawer is contained in lower layer box body, the pumping The outer surface of drawer and the side of corresponding lower layer box body are concordant.
Further, the upper layer box body is equipped with external jack and interior jointing holes, and the external jack is for being electrically connected Power supply, the interior jointing holes are for being electrically connected lid.
Further, thermal insulation layer made of one layer of heat-barrier material is laid on the inner wall of the cryogenic box.
Compared with prior art, the beneficial effects of the present invention are:
A kind of electrophoresis tank convenient for fixed plastic plate of the present invention, including electrophoresis tank and plastic plate, the electrophoresis tank include groove body And lid, groove body both ends are equipped with electrode, the conducting ring being electrically connected to a power source are correspondingly provided on lid, when cover cap is together on groove body When, electrode is connected with power supply, it is possible thereby to carry out electrophoresis.The inner wall of the side of groove body is equipped with the first sliding rail, the first sliding rail Upper end extends at the top of groove body, and the lower end of the first sliding rail extends to groove body bottom, is correspondingly provided with convex on the side of plastic plate Sliding block out, when sliding block from top to bottom slides into the first sliding rail, at this point, plastic plate is fixed in groove body, also, plastic plate with Electrophoresis tank is parallel, and plastic plate can not be moved or be rotated, and ensure that the linearity of electrophoretic band, will not go out between adjacent ribbons The case where now intersecting.After the completion of electrophoresis, the sliding block of plastic plate can be skidded off along the first sliding rail from the bottom to top, and then taken out Running gel is used for imaging.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the electrophoresis tank convenient for fixed plastic plate of one embodiment of the present invention;
Fig. 2 is the disassemblying structure schematic diagram of electrophoresis tank shown in Fig. 1;
Fig. 3 is the partial enlarged view of the first sliding rail shown in Fig. 2;
Fig. 4 is the structural schematic diagram of groove body shown in Fig. 2;
Fig. 5 is the structural schematic diagram of cryogenic box.
In figure: 10, electrophoresis tank;110, groove body;111, electrode;112, the first sliding rail;113, it is horizontally arranged platform;120, it covers Body;121, conducting ring;20, plastic plate;21, sliding block;30, cryogenic box;31, upper layer box body;311, external jack;32, lower layer's case Body;321, drawer;3211, handle;33, horizontal grate;34, thermal insulation layer.
Specific embodiment
In the following, being described further in conjunction with attached drawing and specific embodiment to the present invention, it should be noted that not Under the premise of conflicting, new implementation can be formed between various embodiments described below or between each technical characteristic in any combination Example.
Embodiment 1
As shown in Figure 1, for the electrophoresis tank convenient for fixed plastic plate of one embodiment of the present invention.As shown in Fig. 2, for figure The dismantling state diagram of electrophoresis tank shown in 1.
As shown in Figure 1-3, should include electrophoresis tank 10 and plastic plate 20 convenient for the electrophoresis tank of fixed plastic plate, electrophoresis tank 10 wraps Groove body 110 and lid 120 are included, 110 both ends of groove body are equipped with electrode 111, are correspondingly provided with the conduction being electrically connected to a power source on lid 120 Ring 121.When carrying out electrophoresis, when lid 120 can be covered on groove body 110, electrode 111 is connected with power supply as a result, groove body It is formed between two end electrodes 111 in 110 and is uniformly distributed electric field, electrically charged nucleic acid or protein molecular in the effect of electric field force Under perhaps pass through the separation that gel completes nucleic acid or protein molecular from left end to right end from right to left side.Specific real It applies in mode, is additionally provided with the first sliding rail on the inner wall of the side (i.e. the two sides vertical with the electric field line of formation) of groove body 110 112, the upper end of the first sliding rail 112 extends to the top of groove body 110, correspondingly, plastic plate 20 side (i.e. with sample when electrophoresis The vertical two sides of moving direction, for being clamped with the first sliding rail 112) on be correspondingly provided with the sliding block 21 outwardly protruded, it is sliding Block 21 can be slided up and down along the first sliding rail 112.When sliding block 21 from top to bottom slides into the first sliding rail 112, plastic plate 20 and slot Body 110 is parallel, at this point, can be good at fixing plastic plate 20 on electrophoresis direction by the first sliding rail 112, plastic plate 20 is not It can be moved or be rotated on electrophoresis direction, advantageously ensure that the linearity of band, simultaneously as sliding block 21 is set to glue The two sides of plate 20 have no effect on the path of electrophoresis, i.e., do not interfere electrophoretic buffer to pass through from running gel surface.In the present embodiment In, the first sliding rail 112 is equipped on the inner wall of the two sides of groove body 110, correspondingly, being all provided on the two sides of plastic plate 20 oriented The sliding block 21 of outer protrusion slides into the first sliding rail 112 simultaneously by the sliding block 21 of two sides, fixes plastic plate 20 from both ends, have Better fixed effect further ensures the linearity of electrophoretic band.In other embodiments, conducting ring 121 can also herein To be replaced by contact tube, when lid 120 is covered on groove body 110, contact tube is socketed on electrode 111, ensure that electrode 111 with Power supply is well connected.
As preferred embodiment, as Figure 2-3, the first sliding rail 112 on the two sides of groove body 110 is in vertical Direction setting.When plastic plate 20 is installed as a result, it is only necessary to which being inserted into plastic plate 20 straight down can be real in the first sliding rail 112 Now fix plastic plate 20;It when extracting plastic plate 20, also only needs straight up to extract plastic plate 20, installation and withdrawal process letter It is single, quick, facilitate experimenter to operate.
As preferred embodiment, the length of the first sliding rail 112 is 20mm.It is in electrophoresis process, plastic plate 20 is vertical It is inserted downwardly into the bottom of the first sliding rail 112, at this point, the distance at top of the plastic plate 20 apart from groove body 110 is 20mm, when filling When electrophoretic buffer, plastic plate 20 is unlikely to sink to the bottom of groove body 110.It, can by the way that the first sliding rail 112 of 20mm long is arranged While guaranteeing that gel is submerged by electrophoretic buffer, it is unlikely to influence experimenter in electrophoresis process to glue because submergence is too deep The observation of on piece electrophoretic band.In other embodiments, the length of the first sliding rail 112 is that 15~25mm can reach above-mentioned effect Fruit.
As preferred embodiment, as shown in figure 4, the bottom surface of groove body 110 is used for the formation of the inner recess of groove body 110 The horizontal positioned platform 113 of plastic plate 20 is placed, the bottom surface of groove body 110 forms "Ji" type sunken inside, the first sliding rail 112 as a result, On the two sides of horizontal positioned platform 113, also, 112 lower end of the first sliding rail extends to horizontal positioned platform 113.As a result, as general When plastic plate 20 is from top to bottom inserted into the first 112 bottom of sliding rail, the bottom surface of plastic plate 20 is against horizontal positioned platform 113, further When ensuring that plastic plate 20 is put into groove body 110, plastic plate 20 is in horizontal positioned state, and then ensures in plastic plate 20 Electric field force suffered by running gel is identical, guarantees the real reliability of experimental result.
As preferred embodiment, as shown in figure 5, should further include for accommodating electricity convenient for the electrophoresis tank of fixed plastic plate Swim the cryogenic box 30 of slot 10, cryogenic box 30 includes upper layer box body 31 and lower layer box body 32, upper layer box body 31 and lower layer box body 32 it Between be equipped with horizontal grate 33, horizontal grate 33 is equipped with several airflow holes, for carry out upper layer box body 31 and lower layer box body 32 it Between heat exchange.Upper layer box body 31 and lower layer box body 32 are separated by horizontal grate 33.Wherein, it is set in lower layer box body 32 Have drawer 321, when drawer 321 is relative to upper 31 stretching motion of layer box body, the selectivity of drawer 321 be contained in lower layer box body 32 or Person pulls out outside lower layer box body 32.When carrying out electrophoresis, first drawer 321 can be pulled out outside lower layer box body 32, and layer box body 32 down Ice cube is added, then drawer 321 is promoted in lower layer box body 32.Further, electrophoresis tank 10 is placed on horizontal grate 33, is covered low The upper cover of incubator 30, access power supply can carry out electrophoresis.By the way that electrophoresis tank 10 to be placed in cryogenic box 30, can effectively absorb The heat generated when a large amount of heat generated in electrophoresis process, especially long-time electrophoresis or high-voltage pulse electrophoresis, so that electrophoresis Band, which is unlikely to spread apart easily, to be come, and ensure that the linearity of electrophoretic band.
As preferred embodiment, lower layer box body 32 is equipped with horizontal slide rail, and horizontal slide rail is perpendicular to lower layer box body 32 Leading flank when opening cryogenic box 30 (i.e., side that user front faces), 321 bottom of drawer is correspondingly provided with idler wheel, and rolls Wheel is set on horizontal slide rail.In use, idler wheel is along horizontal when drawer 321 is promoted or pulled out lower layer box body 32 by user Sliding rail rolls.Thus, it is ensured that experimenter easily can pull open or close drawer 321, facilitate experimenter as needed Ice cube is added.
As preferred embodiment, as shown in figure 5, being additionally provided with handle 3211 on the outer surface of drawer 321.In use, Experimenter can hold the handle 3211 and be pushed into lower layer box body 32 or pull out from lower layer box body 32.When drawer 321 is received When being dissolved in lower layer box body 32, the outer surface of drawer 321 (i.e. pull-out drawer 321 when, the side that user front faces, that is, Surface locating for handle 3211) it is concordant with the leading flank of lower layer box body 32.On the one hand, it is ensured that ice cube is completely enclosed within low temperature In case 30, the cryogenic effect in cryogenic box 30 ensure that;On the other hand, the front side of the outer surface of drawer 321 and lower layer box body 32 Face is concordant, also can guarantee the leading flank whole smooth of lower layer box body 32, is convenient for carrying.
As preferred embodiment, as shown in figure 5, the outer wall of upper layer box body 31 is equipped with external jack 311, it is corresponding , interior jointing holes are additionally provided on the inner wall of upper layer box body 31, effect is conducted by external jack 311 and interior jointing holes, makes The electrophoresis tank 10 is connected with external power supply, external jack 311 and interior jointing holes further ensure the sealing of cryogenic box 30 with Thermal insulation is conducive to maintain the low temperature in cryogenic box 30 for a long time.
As preferred embodiment, as shown in figure 5, be laid on the inner wall of cryogenic box 30 made of one layer of heat-barrier material every Thermosphere 34.It can guarantee that the temperature long period in cryogenic box 30 keeps low temperature level by the way that thermal insulation layer 34 is arranged, meet big point Long-time electrophoresis requirement when sub- electrophoresis.
Embodiment 2
Embodiment 2 difference from example 1 is that: the first sliding rail 112 on the two sides of groove body 110, which tilts, to be set It sets, in the present embodiment, the first sliding rail 112 tilts 45 °.When installing plastic plate 20 as a result, by plastic plate 20 along the first sliding rail In 112 inclination insertion groove bodies 110, insertion process resistance is relatively small, meanwhile, the wave that electrophoretic buffer output will not be made excessive Line.Meanwhile when extracting plastic plate 20, plastic plate 20 is tilted along the first sliding rail 112 and extracts groove body 110, withdrawal process resistance phase To smaller, meanwhile, the ripple that electrophoretic buffer output will not be made excessive.
Embodiment 3
Embodiment 3 difference from example 1 is that: one side (the i.e. two sides vertical with electric field line of groove body 110 In any one side) inner wall on be additionally provided with the first sliding rail 112, the upper end of the first sliding rail 112 extends to the top of groove body 110 Portion, correspondingly, the one side (any one side i.e. in the two sides vertical with sample moving direction when electrophoresis) of plastic plate 20 On be correspondingly provided with the sliding block 21 outwardly protruded, sliding block 21 can be slided up and down along the first sliding rail 112.By the way that plastic plate 20 will be set to Side on sliding block 21 be inserted into the first sliding rail 112 on the side of groove body 110, it is same have fix glue by being clamped The effect of plate 20 ensure that the linearity of electrophoretic band.
The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto, The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention Claimed range.

Claims (10)

1. a kind of electrophoresis tank convenient for fixed plastic plate, which is characterized in that including electrophoresis tank and plastic plate, the electrophoresis tank includes Groove body and lid, the groove body both ends are equipped with electrode, the conducting ring being electrically connected to a power source are correspondingly provided on the lid, when described When cover cap is together on groove body, the electrode is connected with power supply;
The inner wall of the side of the groove body is equipped with the first sliding rail, and the upper end of first sliding rail extends at the top of groove body, described The sliding block of protrusion is correspondingly provided on the side of plastic plate, when sliding block from top to bottom slides into the first sliding rail, the plastic plate and slot Body is parallel.
2. the electrophoresis tank as described in claim 1 convenient for fixed plastic plate, which is characterized in that first sliding rail is in vertical side To setting.
3. the electrophoresis tank convenient for fixed plastic plate as claimed in claim 2, which is characterized in that the length of first sliding rail is 15~25mm.
4. the electrophoresis tank as claimed in claim 3 convenient for fixed plastic plate, which is characterized in that the groove body bottom surface is into groove body Portion is recessed the horizontal positioned platform to be formed for placing plastic plate, and first sliding rail lower end extends to horizontal positioned platform.
5. the electrophoresis tank as claimed in claim 4 convenient for fixed plastic plate, which is characterized in that first sliding rail includes one Right, a pair of first sliding rail is to being set on the two sides of horizontal positioned platform, correspondingly, sliding block is on the two sides of plastic plate.
6. the electrophoresis tank as described in any one in claim 1-5 convenient for fixed plastic plate, which is characterized in that described convenient for fixation The electrophoresis tank of plastic plate further includes the cryogenic box for accommodating electrophoresis tank, and the cryogenic box includes upper layer box body and lower layer box body, Horizontal grate is equipped between the upper layer box body and lower layer box body;
Drawer is equipped in the lower layer box body, when the drawer is relative to upper layer bin movement, drawer is selectively contained in lower layer In cabinet or pull out outside lower layer box body.
7. the electrophoresis tank as claimed in claim 6 convenient for fixed plastic plate, which is characterized in that the lower layer box body is equipped with water Smooth rail, either side of the horizontal slide rail perpendicular to lower layer box body;
The drawer bottom is equipped with idler wheel, and the idler wheel is rolled along horizontal slide rail.
8. the electrophoresis tank as claimed in claim 7 convenient for fixed plastic plate, which is characterized in that on the outer surface of the drawer also Equipped with handle, when drawer is contained in lower layer box body, the outer surface of the drawer and the side of corresponding lower layer box body are concordant.
9. the electrophoresis tank as claimed in claim 8 convenient for fixed plastic plate, which is characterized in that the upper layer box body is equipped with outer Jointing holes and interior jointing holes, the external jack are used for electric connection of power supply, and the interior jointing holes are for being electrically connected lid.
10. the electrophoresis tank as claimed in claim 9 convenient for fixed plastic plate, which is characterized in that on the inner wall of the cryogenic box It is laid with thermal insulation layer made of one layer of heat-barrier material.
CN201810292970.1A 2018-03-30 2018-03-30 A kind of electrophoresis tank convenient for fixed plastic plate Pending CN110161107A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810292970.1A CN110161107A (en) 2018-03-30 2018-03-30 A kind of electrophoresis tank convenient for fixed plastic plate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810292970.1A CN110161107A (en) 2018-03-30 2018-03-30 A kind of electrophoresis tank convenient for fixed plastic plate

Publications (1)

Publication Number Publication Date
CN110161107A true CN110161107A (en) 2019-08-23

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CN201810292970.1A Pending CN110161107A (en) 2018-03-30 2018-03-30 A kind of electrophoresis tank convenient for fixed plastic plate

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830725A (en) * 1987-08-04 1989-05-16 Life Technologies, Inc. Electrophoresis apparatus
JPH10293119A (en) * 1997-04-17 1998-11-04 Toyobo Co Ltd Electrophoretic unit
TW530943U (en) * 2000-09-06 2003-05-01 Wealtec Bioscience Co Ltd Gelatine disc structure of electrophoresis slot
CN202670411U (en) * 2012-05-15 2013-01-16 苏州国环环境检测有限公司 Portable sample box capable of refrigerating
CN105116042A (en) * 2015-09-25 2015-12-02 贵州大学 PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) gel electrophoresis low-temperature operation box
CN206161585U (en) * 2016-08-29 2017-05-10 天津市康婷生物工程有限公司 Novel nucleic acid electrophoresis tank

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830725A (en) * 1987-08-04 1989-05-16 Life Technologies, Inc. Electrophoresis apparatus
JPH10293119A (en) * 1997-04-17 1998-11-04 Toyobo Co Ltd Electrophoretic unit
TW530943U (en) * 2000-09-06 2003-05-01 Wealtec Bioscience Co Ltd Gelatine disc structure of electrophoresis slot
CN202670411U (en) * 2012-05-15 2013-01-16 苏州国环环境检测有限公司 Portable sample box capable of refrigerating
CN105116042A (en) * 2015-09-25 2015-12-02 贵州大学 PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) gel electrophoresis low-temperature operation box
CN206161585U (en) * 2016-08-29 2017-05-10 天津市康婷生物工程有限公司 Novel nucleic acid electrophoresis tank

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Application publication date: 20190823