CN110157612B - Photosynthetic bacteria culture-light fermentation hydrogen production combined reactor and method for producing hydrogen by using same - Google Patents
Photosynthetic bacteria culture-light fermentation hydrogen production combined reactor and method for producing hydrogen by using same Download PDFInfo
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Abstract
The invention discloses a photosynthetic bacteria culture-light fermentation hydrogen production combined reactor and a method for producing hydrogen by using the same, wherein the combined reactor comprises a cylinder body and a box body, the cylinder body consists of a top plate, a bottom plate and a cylindrical side wall, a first cylindrical partition and a second cylindrical partition are sequentially arranged in the cylinder body from outside to inside, so that a photosynthetic bacteria culture area, a heat preservation illumination area and a light fermentation hydrogen production area are sequentially formed from outside to inside, a first partition and a second partition are sequentially arranged in the box body from left to right, a growth culture medium chamber, a clear water chamber and a hydrogen production substrate chamber are sequentially formed from left to right, the growth culture medium chamber is communicated with the photosynthetic bacteria culture area through a hose, the clear water chamber is communicated with the heat preservation illumination area through a hose, and the hydrogen production substrate chamber is communicated with the light fermentation hydrogen production area through a hose. The invention can realize that photosynthetic bacteria culture and photo-fermentation hydrogen production share one set of illumination and heat preservation device, and realizes the maximum utilization of light energy and heat energy.
Description
Technical Field
The invention relates to the technical field of hydrogen production by biomass photosynthetic organisms, in particular to a photosynthetic bacteria culture-light fermentation hydrogen production combined reactor and a method for producing hydrogen by using the same.
Background
Since the twenty-first century, energy shortage and environmental pollution are just like two mountains are stopped in the front of all mankind, and therefore, development and discussion of new clean energy are imperative. As an important material foundation, the development and survival of human beings can not leave the energy source, along with the progress of society, at the present day that the economic development is different day by day, the problems related to the energy source attract the attention of the whole world, and the development of novel sustainable novel clean energy with high efficiency and low cost becomes one of the key points of the development of the current society. In the face of the important subject of the twenty-first century, each country brings the development of new energy into new strategic planning of the country, and even makes relevant national policies. In the field of new energy, clean and pollution-free hydrogen energy utilization technology, which has been the focus of industrial importance, is being developed at a remarkable rate, and many developed countries have greatly accelerated research speed considering "clean" hydrogen energy as a future energy source in this country. Hydrogen energy is itself a secondary energy source that can only be extracted from the primary energy source by some technical means. As an ideal alternative energy source, hydrogen energy has many advantages, such as: good combustion performance, high heat value, rich resources and the like; as an ideal alternative energy source, biological hydrogen production is widely concerned by countries in the world due to the advantages of less pollution, mild reaction conditions, low energy consumption and the like.
In biological hydrogen production, the advantages of photosynthetic bacteria hydrogen production are obvious: the bacteria can degrade organic wastes to generate hydrogen, hydrogen production is combined with waste removal, resources are recycled, solar light energy can be effectively converted into hydrogen energy, the hydrogen energy is further converted into electric energy by a fuel cell, and the whole conversion process is pollution-free, low in energy consumption and simple to operate. And various photosynthetic bacteria exist widely in the environment, are easy to obtain, have higher hydrogen production efficiency and higher light conversion rate. Therefore, the hydrogen production by the photosynthetic organisms has certain economic benefits and certain environmental benefits, and is a hydrogen production mode with great development prospect. The hydrogen production by photosynthetic organisms at present still stays in the research stage of a laboratory, and research on hydrogen production reactors by light fermentation is an important ring for the hydrogen production technology by photosynthetic organisms from the laboratory to the industrialization.
Disclosure of Invention
The invention aims to provide a photosynthetic bacteria culture-light fermentation hydrogen production combined reactor and a method for producing hydrogen by using the same. The device can realize photosynthetic bacteria culture and one set of illumination and heat preservation device of hydrogen sharing of light fermentation, has realized the maximize utilization to light energy and heat energy, and has increased photosynthetic bacteria's photic area, has reduced the heat loss of the hydrogen of light fermentation, reduces the energy consumption, has improved the utilization efficiency to the energy, has reduced the running cost. The bacteria culture area and the light fermentation hydrogen production area are communicated by the electromagnetic valve, so that the operation of photosynthetic bacteria inoculation is conveniently carried out on the light fermentation hydrogen production area, and the test workload is reduced.
Based on the purpose, the invention adopts the following technical scheme:
a photosynthetic bacteria culture-light fermentation hydrogen production combined reactor comprises a cylinder body arranged on a first support and a box body arranged on a second support, wherein the cylinder body consists of a top plate, a bottom plate and a cylindrical side wall arranged between the bottom plate and the top plate, a first cylindrical partition and a second cylindrical partition are sequentially arranged in the cylinder body from outside to inside, a photosynthetic bacteria culture area is formed between the cylindrical side wall and the first cylindrical partition, a heat preservation illumination area is formed between the first cylindrical partition and the second cylindrical partition, a light fermentation hydrogen production area is formed in the second cylindrical partition, a first partition and a second partition are sequentially arranged in the box body from left to right, a growth culture medium chamber is formed in a space between the first partition and the left side wall of the box body, a clear water chamber is formed in a space between the first partition and the second partition, and a hydrogen production substrate chamber is formed in a space between the second partition and the right side wall of the box body, the growth culture medium chamber is communicated with the photosynthetic bacteria culture area through a hose, the clear water chamber is communicated with the heat preservation illumination area through a hose, the hydrogen production substrate chamber is communicated with the light fermentation hydrogen production area through a hose, temperature sensors are arranged in the photosynthetic bacteria culture area, the heat preservation illumination area and the light fermentation hydrogen production area, and the three temperature sensors are respectively connected with a temperature controller.
Furthermore, a stirring mechanism is arranged in the second cylindrical partition, the stirring mechanism comprises a motor, stirring blades and a stirring shaft, the motor is arranged on the top plate, the stirring shaft penetrates through the top plate, the top of the stirring shaft is connected with the motor, the stirring shaft extends downwards into the lower part of the hydrogen production area through light fermentation, the stirring blades are fixed on the stirring shaft, a light source (specifically, an LED lamp belt is wound on the outer wall of the second cylindrical partition), a hydrogen production outlet pipeline is arranged on the top plate corresponding to the hydrogen production area through light fermentation, a gas flow meter is arranged on the hydrogen production outlet pipeline, an electric heating wire is arranged on the bottom plate corresponding to the heat preservation illumination area, a communicating pipe is arranged at the lower parts of the photosynthetic bacteria culture area and the hydrogen production area, and an electromagnetic valve is arranged on the communicating pipe; each hose is provided with a stop valve and a peristaltic pump.
Furthermore, the top of the growth medium chamber, the clear water chamber and the hydrogen production substrate chamber is provided with a feed inlet, the lower parts of the growth medium chamber, the clear water chamber and the hydrogen production substrate chamber are respectively provided with a sample outlet pipeline, a sample outlet valve is arranged on the sample outlet pipeline, the lower parts of the cylindrical side wall, the first cylindrical partition and the second cylindrical partition are respectively provided with a discharge pipeline, and a discharge valve is arranged on the discharge pipeline.
Further, barrel, first cylindrical wall, second cylindrical wall, boxThe first partition plate and the second partition plate are made of transparent materials, specifically glass or organic glass, and the volumes of the photosynthetic bacteria culture area, the heat preservation illumination area and the light fermentation hydrogen production area are respectively marked as V 1 、V 2 、V 3 (ii) a And V is 2 :V 1 ≥1，V 1 :V 3 More than or equal to 1:2, and the volumes of the growth medium chamber, the clear water chamber and the hydrogen production substrate chamber are respectively marked as V 4 、V 5 、V 6 (ii) a The ratio of the volume of each chamber to the volume of the corresponding zone in communication therewith is equal to or greater than 6:5, i.e. V 4 :V 1 ≥6:5，V 5 :V 2 ≥6:5，V 6 :V 3 ≥6:5。
The method for producing hydrogen by using the photosynthetic bacteria culture-light fermentation hydrogen production combined reactor comprises the following steps:
a: respectively adding a photosynthetic bacteria growth culture medium and clear water prepared in proportion into the growth culture medium chamber and the clear water chamber through a feeding hole, switching on a power supply of a peristaltic pump, respectively conveying the photosynthetic bacteria growth culture medium and the clear water to a heat preservation illumination area, and culturing for 48-72 hours by connecting a light source and a power supply of an electric heating wire;
b: adding hydrogen production matrix reaction liquid into the hydrogen production matrix chamber through a feed port; switching on a power supply of a peristaltic pump, and adding hydrogen-producing substrate reaction liquid into a light fermentation hydrogen-producing area; switching on a power supply of an electromagnetic valve, and conveying photosynthetic bacteria from the photosynthetic bacteria culture area to the photo-fermentation hydrogen production area, wherein the conveying amount of the photosynthetic bacteria is 20-30% of the total volume of the reaction;
c: a hydrogen storage tank is arranged at the outlet of the hydrogen production outlet pipeline, and the volume of produced gas is recorded by a gas flowmeter;
d: and (5) closing the valve, and increasing the gas production rate by using an intermittent stirring mode.
Preferably, the photosynthetic bacteria strain is from HAU-M1 photosynthetic hydrogen-producing bacteria provided by Henan university of agriculture and consists of 9% of rhodobacter sphaeroides, 27% of rhodospirillum rubrum, 11% of rhodobacter capsulatum, 28% of Rhodopseudomonas palustris and 25% of Rhodopseudomonas capsulatum in quantitative ratio.
Preferably, the photosynthetic bacteria growth medium is specifically composed of: 0.5 g/L yeast extract, 1.0 g/L NaCl, MgSO 4 ·7H 2 O 0.1 g/L，CH 3 COONa 2.0 g/L，K 2 HPO 4 0.1 g/L，NaHCO 3 1.0 g/L，NH 4 0.5 g/L of Cl and the balance of distilled water.
Preferably, the hydrogen-producing substrate reaction liquid is composed of: mixing sodium glutamate, NaCl and K 2 HPO 4 Yeast extract, MgCl 2 、NH 4 Adding Cl, biomass straw powder and cellulase into a buffer solution to ensure that the concentration of sodium glutamate is 3.56 g/L, the concentration of NaCl is 2.0 g/L and K is 2 HPO 4 The concentration is 0.50 g/L, the yeast extract concentration is 0.10 g/L, and MgCl is adopted 2 Concentration of 0.20 g/L, NH 4 The Cl concentration is 0.40 g/L, cellulase is added according to 100mg/g of straws, biomass straw powder is added according to an effective volume of 25g/L, and the mixture is uniformly mixed, wherein the effective volume is the sum of the reaction liquid volume of the hydrogen-producing substrate and the volume of the added photosynthetic bacteria; wherein the buffer is a citric acid-sodium citrate solution at pH = 4.8.
Further, the reaction temperature of the photosynthetic bacteria culture area and the reaction temperature of the photo-fermentation hydrogen production area are both 30 +/-1 ℃; the illumination intensity of the LED lamp strip is 2500-3500 lux.
The device can realize that photosynthetic bacteria culture and photo-fermentation hydrogen production share one set of illumination and heat preservation device, realize the maximum utilization of light energy and heat energy, increase the light receiving area of photosynthetic bacteria, reduce the heat loss of photo-fermentation hydrogen production, reduce energy consumption and improve the utilization efficiency of energy; the bacteria culture layer and the light fermentation layer are communicated by the electromagnetic valve, so that the operation of inoculating photosynthetic bacteria on the light fermentation layer is facilitated, and the test workload is reduced; the culture medium box and the hydrogen production substrate box are communicated by the peristaltic pump, so that the conventional hydrogen production process is simplified. In addition, the device has the advantages of small floor area, simple operation method, easy realization of modernization, wide application in the industries of energy, environmental protection and the like, and good application prospect.
Drawings
FIG. 1 is a schematic structural diagram of a combined reactor for culturing photosynthetic bacteria and producing hydrogen by light fermentation according to the invention;
FIG. 2 is a schematic structural view of the cylindrical barrel of FIG. 1;
in the figure: 1. the device comprises a first support, a bottom plate, a base plate, an electric heating wire, a communicating pipe, a magnetic valve, a temperature controller, a temperature sensor, a photosynthetic bacteria culture area, a heat preservation illumination area, a light fermentation hydrogen production area, a stirring shaft, a motor, a hydrogen production outlet pipeline, a gas flowmeter, a stirring blade, an LED lamp strip, a second cylindrical partition, a gas flowmeter, a stirring blade, a light source, a light.
Detailed Description
The following further description is made with reference to the accompanying drawings, and the present embodiment is based on the technical solution and provides a detailed implementation and a specific operation process.
A photosynthetic bacteria culture-light ferment hydrogen production combined reactor, as shown in figure 1 and figure 2, comprises a cylindrical barrel arranged on a first support 1 and a rectangular box arranged on a second support 31, the cylindrical barrel is composed of a top plate 32, a bottom plate 2 and a cylindrical side wall 19 arranged between the bottom plate 2 and the top plate 32, a first cylindrical partition 18 and a second cylindrical partition 17 are sequentially arranged in the cylindrical barrel from outside to inside, a photosynthetic bacteria culture area 8 is formed between the cylindrical side wall 19 and the first cylindrical partition 18, a heat preservation illumination area 9 is formed between the first cylindrical partition 18 and the second cylindrical partition 17, a light ferment hydrogen production area 10 is formed in the second cylindrical partition 17, a first partition and a second partition are sequentially arranged in the rectangular box from left to right, and a growth culture medium chamber 26 is formed in the space between the first partition and the left side wall of the box, the space between the first clapboard and the second clapboard forms a clear water chamber 27, the space between the second clapboard and the right side wall of the box body forms a hydrogen production substrate chamber 28, the growth culture medium chamber 28 is communicated with the photosynthetic bacteria culture area 8 through a hose 23, the clear water chamber 27 is communicated with the heat preservation illumination area 9 through a hose 23, the hydrogen production substrate chamber 28 is communicated with the light fermentation hydrogen production area 20 through a hose 23, the photosynthetic bacteria culture area 8, the heat preservation illumination area 9 and the light fermentation hydrogen production area 10 are internally provided with temperature sensors 7, and the three temperature sensors 7 are respectively connected with a temperature controller 6. The temperature controller 6 is connected to an external control terminal to transmit signals to the control center, which is a technical means known in the art and is not the point of the invention, and will not be described herein.
Further, a stirring mechanism is arranged in the second cylindrical partition 17 and comprises a motor 12, a stirring blade 15 and a stirring shaft 11, the motor 12 is arranged on the top plate 32, the stirring shaft 11 penetrates through the top plate 32, the top of the stirring shaft 11 is connected with the motor 12, the stirring shaft 11 extends downwards to extend into the lower portion of the light fermentation hydrogen production area 10, the stirring blade 15 is fixed on the stirring shaft 11, an LED lamp strip 16 is fixedly wound on the outer wall of the second cylindrical partition 17, a hydrogen production outlet pipeline 13 is arranged on the top plate corresponding to the light fermentation hydrogen production area 10, a gas flowmeter 14 is arranged on the hydrogen production outlet pipeline 13, an electric heating wire 3 is arranged on the bottom plate corresponding to the heat preservation illumination area 9, a communicating pipe 4 is arranged at the lower portions of the photosynthetic bacteria culture area 8 and the light fermentation hydrogen production area 10, and an electromagnetic valve 5 is arranged on the communicating pipe 4; each hose 23 is provided with a shut-off valve 22 and a peristaltic pump 24.
Furthermore, the top of the growth medium chamber 26, the top of the clean water chamber 27 and the top of the hydrogen-producing substrate chamber 28 are provided with a feed inlet 25, the lower parts of the growth medium chamber 26, the clean water chamber 27 and the lower part of the hydrogen-producing substrate chamber 28 are respectively provided with a sample outlet pipeline 29, the sample outlet pipeline 29 is provided with a sample outlet valve 30, the lower parts of the cylindrical side wall 19, the first cylindrical partition 18 and the second cylindrical partition 17 are respectively provided with a discharge pipeline 20, and the discharge pipeline 20 is provided with a discharge valve 21.
Further, the cylindrical barrel, the first cylindrical partition 18, the second cylindrical partition 17, the rectangular box body, the first partition and the second partition are all made of transparent materials, such as glass or organic glass, and the volumes of the photosynthetic bacteria culture area 8, the heat preservation illumination area 9 and the light fermentation hydrogen production area 10 are respectively marked as V 1 、V 2 、V 3 (ii) a And V is 2 :V 1 ≥1，V 1 :V 3 Not less than 1:2, the volumes of the growth medium chamber 26, the clear water chamber 27 and the hydrogen-producing substrate chamber 28 are respectively marked as V 4 、V 5 、V 6 (ii) a The ratio of the volume of each chamber to the volume of the corresponding zone in communication therewith is equal to or greater than 6:5, i.e. V 4 :V 1 ≥6:5，V 5 :V 2 ≥6:5，V 6 :V 3 ≥6:5。
In this embodiment, the length of the first support 1 is 400 mm, the width is 400 mm, the height is 500 mm, the height of the cylindrical barrel is 1000 mm, the radius is 180 mm, the radius of the first cylindrical partition 18 is 150 mm, and the radius of the second cylindrical partition 17 is 100 mm. The cylindrical barrel, the first cylindrical partition 18 and the second cylindrical partition 17 are made of organic glass.
In this embodiment, the pitch of the uniformly wound turns of the lighting LED strip 16 is 80 mm, the fixed radius of the electric heating wire 3 is 165 mm, and the power is 2000W.
In this embodiment, the stirring shaft 11 is a metal rod with a length of 800 mm, a first stirring blade 15 is welded at a position 350 mm away from the lower end of the motor 12, and a second stirring blade 15 is welded at a position 700 mm away from the lower end of the motor 12.
In this embodiment, the second support 31 has a length of 500 mm, a width of 500 m, and a height of 500 mm; the growth medium chamber 26 has a length (length in the front-rear direction, the same applies hereinafter) of 500 mm, a width (length in the left-right direction, the same applies hereinafter) of 130 mm, and a height of 600 mm; the length of the clean water chamber 27 is 500 mm, the width is 160 mm, and the height is 600 mm; the hydrogen production substrate chamber 28 is 500 mm long, 130 mm wide and 600 mm high; the radius of the feed port 25 is 60 mm; the rectangular box body, the first partition plate and the second partition plate are made of organic glass.
The hydrogen production method utilizing the combined reactor comprises the following steps:
1. photosynthetic bacteria growth culture medium and clear water which are prepared in proportion are respectively added into the growth culture medium chamber 26 and the clear water chamber 27 through the feeding hole 25, the power supply of the peristaltic pump 24 is switched on and is respectively transmitted to the photosynthetic bacteria culture area 8 and the heat preservation illumination area 9, the power supply of the LED lamp strip 16 and the electric heating wire 3 is switched on, and the culture is carried out for 48 hours.
2. Adding hydrogen-producing substrate reaction liquid into the hydrogen-producing substrate chamber 28 through the feed port 25; switching on a power supply of a peristaltic pump 24, and adding hydrogen-producing substrate reaction liquid into the light fermentation hydrogen-producing area 10; switching on a power supply of the electromagnetic valve 5, and conveying photosynthetic bacteria from the photosynthetic bacteria culture area 8 to the photo-fermentation hydrogen production area 10, wherein the volume of the photosynthetic bacteria accounts for 20-30% of the volume of the photo-fermentation hydrogen production area 10; the hydrogen production substrate reaction liquid accounts for 70-80% of the volume of the photo-fermentation hydrogen production area 10; the photosynthetic bacteria and the hydrogen-producing substrate reaction liquid which are conveyed to the light fermentation hydrogen-producing area 10 are mixed, and the photosynthetic bacteria utilizes the reducing sugar in the hydrogen-producing substrate reaction liquid to carry out the photosynthetic hydrogen production under the illumination effect of the LED lamp strip 16. Then, a growth medium is added into the photosynthetic bacteria culture area 8, and photosynthetic bacteria are inoculated for continuous culture for the next use.
3. The generated hydrogen is gathered to the top of the hydrogen producing area 10 by light fermentation under the action of buoyancy and is led out from the outlet of the arranged hydrogen outlet pipeline 13, a hydrogen storage tank is arranged at the gas production outlet for storing the generated hydrogen, and the gas flow meter 14 records the gas production volume.
4. Setting the hydraulic retention period of the system to be 48 h, improving the gas production rate by using an intermittent stirring mode, and stirring once every 4 h for 30 min.
In this embodiment, the photosynthetic bacteria growth medium added in step 1 consists of: distilled water, yeast extract 0.5 g/L, NaCl 1.0 g/L, MgSO 4 ·7H 2 O 0.1 g/L，CH 3 COONa 2.0 g/L，K 2 HPO 4 0.1 g/L，NaHCO 3 1.0 g/L，NH 4 Cl 0.5 g/L. The amount of the photosynthetic bacteria added in the photosynthetic bacteria culture area 8 is 40% of the volume of the photosynthetic bacteria culture area 8, and the volume of the photosynthetic bacteria growth culture medium added is 60% of the volume of the photosynthetic bacteria culture area 8.
The photosynthetic bacteria added in the step 1 are inoculated by the photosynthetic bacteria in the later logarithmic phase, and the culture is carried out under the conditions that the illumination intensity is adjusted to be 3000lx and the temperature is 30 ℃.
In this example, the photosynthetic bacteria species were from HAU-M1 photosynthetic hydrogen-producing bacteria supplied by Henan university of agriculture and consisted, in terms of number ratios, of 9% Rhodobacter sphaeroides (Rhodobacter sphaeroides), 27% Rhodospirillum rubrum (Rhodospirillum rubrum), 11% Rhodobacter capsulatus (Rhodobacter capsulatus), 28% Rhodopseudomonas palustris (Rhodopseudomonas pulosum) and 25% Rhodopseudomonas capsulata (Rhodopseudomonas capsulata).
In this example, the composition of the hydrogen-producing substrate reaction solution was: mixing sodium glutamate,NaCl、K 2 HPO 4 Yeast extract, MgCl 2 、NH 4 Adding Cl, biomass straw powder and cellulase into a buffer solution to ensure that the concentration of sodium glutamate is 3.56 g/L, the concentration of NaCl is 2.0 g/L, and K is 2 HPO 4 The concentration is 0.50 g/L, the concentration of yeast extract is 0.10 g/L, MgCl 2 Concentration of 0.20 g/L, NH 4 The Cl concentration is 0.40 g/L, cellulase is added according to 100mg/g of straw, biomass straw powder is added according to an effective volume of 25g/L, and the mixture is uniformly mixed, wherein the effective volume is the sum of the reaction liquid volume of the hydrogen-producing substrate and the volume of the added photosynthetic bacteria.
Buffer solution: the buffer solution is a citric acid-sodium citrate solution with pH =4.8, and the specific preparation process is as follows:
solution A: 21.014 g of citric acid is accurately weighed into a 500 mL beaker, dissolved by a small amount of distilled water and then added to 1000 mL to obtain 0.1 mol/L citric acid solution.
Solution B: 29.412 g of sodium citrate is accurately weighed in a 500 mL beaker, dissolved by a small amount of distilled water and then is added to 1000 mL to obtain a 0.1 mol/L sodium citrate solution.
And (3) taking 230 mL of the solution A and 270 mL of the solution B, fully mixing, transferring into a 1000 mL volumetric flask, metering to 1000 mL by using distilled water, and fully and uniformly mixing.
The volume of the photosynthetic bacteria accounts for 20-30% of the volume of the hydrogen production area for light fermentation; the hydrogen production substrate reaction liquid accounts for 70-80% of the volume of the hydrogen production area 10 of the photo-fermentation, and can be adjusted according to actual experiment requirements.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, and equivalents thereof are intended to be included in the scope of the present invention.
Claims (9)
1. A photosynthetic bacteria culture-light fermentation hydrogen production combined reactor is characterized by comprising a cylinder body arranged on a first support and a box body arranged on a second support, wherein the cylinder body consists of a top plate, a bottom plate and a cylindrical side wall arranged between the bottom plate and the top plate, a first cylindrical partition and a second cylindrical partition are sequentially arranged in the cylinder body from outside to inside, a photosynthetic bacteria culture area is formed between the cylindrical side wall and the first cylindrical partition, a heat preservation illumination area is formed between the first cylindrical partition and the second cylindrical partition, a light fermentation hydrogen production area is formed in the second cylindrical partition, a first partition and a second partition are sequentially arranged in the box body from left to right, a growth culture medium chamber is formed in a space between the first partition and the left side wall of the box body, a clear water chamber is formed in a space between the first partition and the second partition, and a hydrogen production substrate chamber is formed in a space between the second partition and the right side wall of the box body, the growth culture medium chamber is communicated with the photosynthetic bacteria culture area through a hose, the clear water chamber is communicated with the heat preservation illumination area through a hose, the hydrogen production substrate chamber is communicated with the light fermentation hydrogen production area through a hose, temperature sensors are arranged in the photosynthetic bacteria culture area, the heat preservation illumination area and the light fermentation hydrogen production area, and the three temperature sensors are respectively connected with a temperature controller; the outer wall of the second cylindrical partition is fixedly wound with an LED lamp strip, the bottom plate corresponding to the heat-preservation lighting area is provided with an electric heating wire, the lower parts of the photosynthetic bacteria culture area and the light fermentation hydrogen production area are provided with a communicating pipe, and the communicating pipe is provided with an electromagnetic valve.
2. A combined reactor for culturing photosynthetic bacteria and producing hydrogen by light fermentation as claimed in claim 1, wherein the second cylindrical partition is internally provided with a stirring mechanism, the stirring mechanism comprises a motor, a stirring blade and a stirring shaft, the motor is arranged on the top plate, the stirring shaft penetrates through the top plate and is connected with the motor, the stirring shaft extends downwards to the lower part of the light fermentation hydrogen production area, the stirring blade is fixed on the stirring shaft, the top plate corresponding to the light fermentation hydrogen production area is provided with a hydrogen production outlet pipeline, the hydrogen production outlet pipeline is provided with a gas flow meter, and each hose is provided with a stop valve and a peristaltic pump.
3. A photosynthetic bacteria culture-light fermentation hydrogen production combined reactor as claimed in claim 1, wherein the top of the growth medium chamber, the clear water chamber and the hydrogen production substrate chamber is provided with a feed inlet, the lower parts of the growth medium chamber, the clear water chamber and the hydrogen production substrate chamber are respectively provided with a sample outlet pipeline, the sample outlet pipeline is provided with a sample outlet valve, the lower parts of the cylindrical side wall, the first cylindrical partition and the second cylindrical partition are respectively provided with a discharge pipeline, and the discharge pipeline is provided with a discharge valve.
4. The combined reactor for photosynthetic bacteria culture-light fermentation hydrogen production as claimed in claim 1, wherein the cylinder, the first cylindrical partition, the second cylindrical partition, the box body, the first partition and the second partition are all made of transparent materials, and the volumes of the photosynthetic bacteria culture area, the heat preservation illumination area and the light fermentation hydrogen production area are respectively marked as V 1 、V 2 、V 3 (ii) a And V is 2 :V 1 ≥1，V 1 :V 3 More than or equal to 1:2, and the volumes of the growth medium chamber, the clear water chamber and the hydrogen production substrate chamber are respectively marked as V 4 、V 5 、V 6 (ii) a The ratio of the volume of each chamber to the volume of the corresponding zone in communication therewith is equal to or greater than 6:5, i.e. V 4 ：V 1 ≥6:5，V 5 ：V 2 ≥6:5，V 6 ：V 3 ≥6:5。
5. Method for producing hydrogen by using photosynthetic bacteria culture-light fermentation hydrogen-production combined reactor as claimed in any one of claims 1 to 4, characterized by comprising the following steps:
a: respectively adding a photosynthetic bacteria growth culture medium and clear water prepared in proportion into the growth culture medium chamber and the clear water chamber through a feeding hole, switching on a power supply of a peristaltic pump, respectively conveying the photosynthetic bacteria growth culture medium and the clear water to a heat preservation illumination area, switching on a power supply of an LED lamp strip and an electric heating wire, and culturing for 48-72 hours;
b: adding hydrogen-producing substrate reaction liquid into the hydrogen-producing substrate chamber through the feed port; switching on a power supply of a peristaltic pump, and adding hydrogen production substrate reaction liquid into a light fermentation hydrogen production area; switching on a power supply of the electromagnetic valve, and conveying photosynthetic bacteria from the photosynthetic bacteria culture area to the photo-fermentation hydrogen production area;
c: a hydrogen storage tank is arranged at the outlet of the hydrogen production outlet pipeline, and the volume of produced gas is recorded by a gas flowmeter;
d: and (5) closing the valve, and increasing the gas production rate by using an intermittent stirring mode.
6. The method for producing hydrogen as claimed in claim 5, wherein the photosynthetic bacteria strain is HAU-M1 photosynthetic hydrogen-producing bacteria, and HAU-M1 photosynthetic hydrogen-producing bacteria comprises 9% of rhodobacter sphaeroides, 27% of rhodospirillum rubrum, 11% of rhodobacter capsulatum, 28% of rhodopseudomonas palustris and 25% of rhodopseudomonas capsulatum by number ratio.
7. Hydrogen production method according to claim 5, characterized in that the specific composition of the photosynthetic bacteria growth medium is: 0.5 g/L yeast extract, 1.0 g/L NaCl, MgSO 4 ·7H 2 O 0.1 g/L，CH 3 COONa 2.0 g/L，K 2 HPO 4 0.1 g/L，NaHCO 3 1.0 g/L，NH 4 Cl 0.5 g/L, and the balance of distilled water.
8. The hydrogen production method according to claim 5, characterized in that the composition of the hydrogen-producing substrate reaction solution is: mixing sodium glutamate, NaCl and K 2 HPO 4 Yeast extract, MgCl 2 、NH 4 Adding Cl, biomass straw powder and cellulase into a buffer solution to ensure that the concentration of sodium glutamate is 3.56 g/L, the concentration of NaCl is 2.0 g/L, and K is 2 HPO 4 The concentration is 0.50 g/L, the concentration of yeast extract is 0.10 g/L, MgCl 2 Concentration of 0.20 g/L, NH 4 The Cl concentration is 0.40 g/L, cellulase is added according to 100mg/g of straw, biomass straw powder is added according to an effective volume of 25g/L, and the mixture is uniformly mixed, wherein the effective volume is the sum of the reaction liquid volume of the hydrogen-producing substrate and the volume of the added photosynthetic bacteria; wherein the buffer is a citric acid-sodium citrate solution at pH = 4.8.
9. The hydrogen production method as claimed in claim 5, wherein the reaction temperature of the photosynthetic bacteria culture region and the light fermentation hydrogen production region is 30 +/-1 ℃; the illumination intensity of the LED lamp strip is 2500-3500 lux.
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| CN113373051B (en) * | 2021-06-17 | 2023-10-10 | 河南农业大学 | Miniature speed-control type photo-biological hydrogen production reaction device |
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