CN101760479A - Method for producing hydrogen through biomass fermentation and photosynthetic coupling - Google Patents
Method for producing hydrogen through biomass fermentation and photosynthetic coupling Download PDFInfo
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Abstract
The invention belongs to the field of biotechnology, and in particular relates to a method for producing hydrogen by utilizing sweet potato, yam and other biomass fermentation and photosynthetic coupling. The method completes fermentative hydrogen production and photosynthetic hydrogen production in the same reactor, and comprises that: biomass is subjected to heat treatment first; a fermentation medium taking the biomass as a substrate is inoculated with fermentative bacteria; anaerobic fermentative hydrogen production is performed in an environment at the temperature of between 25 and 40 DEG C with a pH between 5.5 and 7.5; and after hydrogen production is completed, fermentation broth does not need to be added with any other nutrient substances or be diluted, and only needs to have pH regulated to be between 6.0 and 7.5 and then be directly inoculated with hybrid photosynthetic flora so as to continue anaerobic photosynthetic hydrogen production with the light intensity between 2,000 and 8, 000 lux. In the method, final fermentation broth after hydrogen production only needs to be added with sweet potatoes, yams and other biomass again and then can be used for coupling hydrogen production again, and the fermentation broth can be reused for 3 to 5 times. The method has the advantages of high hydrogen production efficiency, long duration of hydrogen production, high substrate conversion rate, easiness for industrialization, and the like.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of method of utilizing biomass ferment-photosynthetic lotus root coproduction hydrogen such as Ipomoea batatas, Chinese yam.
Background technology
The biological product hydrogen methods that produces hydrogen as a kind of clean environment firendly has caused that people pay close attention to widely, and the biological hydrogen that produces mainly contains fermentation and hydrogen production and two kinds of methods of photosynthetic hydrogen production.Fermentation and hydrogen production mainly utilizes strict anaerobe and facultative anaerobic bacteria etc. to decompose some larger molecular organicses (as starch, Mierocrystalline cellulose etc.) and produces hydrogen, its hydrogen-producing speed is fast, substrate conversion rate height, but a large amount of VFA that produce in the fermentation and hydrogen production process (acetate, butyric acid and propionic acid) can cause pH to descend, thereby influence hydrogen generation efficiency, the generation of simultaneously a large amount of VFA also can cause certain pollution to environment; And photosynthetic bacterium (as Rhodobactersphaeroides, Rhodopseudomonas sp. and Rhodopseudomonas palustris etc.) carries out photosynthetic hydrogen production with the organic acid behind the fermentation and hydrogen production, its photosynthetic hydrogen production efficient will be far above fermentation and hydrogen production efficient, and photosynthetic bacterium can utilize natural light to produce hydrogen.Therefore,, can make full use of substrate and carry out biology product hydrogen, further improve hydrogen generation efficiency, realize the biological hydrogen industrialization of producing if by fermenting bacteria and photosynthetic bacterium lotus root coproduction hydrogen.
Existing both at home and abroad at present a lot of research and reports about fermentation-photosynthetic lotus root coproduction hydrogen, but still be in the exploratory stage, there are a lot of problems and defective.
At first be to lack to satisfy the low cost product hydrogen substrate that industrial biological produces hydrogen, many early-stage Study all are to utilize pattern substrate such as glucose, sucrose and starch etc. to ferment-photosynthetic lotus root coproduction hydrogen, for example: Haruhiko Y etc. does substrate utilization fermenting bacteria Clostridium butyricum and photosynthetic bacterium Rhodobacter sp.M-19 lotus root coproduction hydrogen with starch, and the maximum hydrogen rate of producing can reach 6.6mol-H
2/ mol-glucose, Chen CY etc. are substrate utilization fermenting bacteria Clostridiumpasteurianum CH4 and photosynthetic bacterium Rhodopseudomonas palustris WP3-5 lotus root coproduction hydrogen with sucrose, the maximum hydrogen rate of producing can reach 10.21mol-H
2/ mol-sucrose, though the lotus root coproduction hydrogen efficiency of these reports is very high, it is actual to utilize pure product hydrogen manufacturing not meet.
Next is at the biological substrate problem of producing hydrogen of restriction, the investigator sight invested the source more extensive, biomass that cost is cheaper and organic solid waste, utilize the method for fermentation of biomass and solid organic castoff and photosynthetic coupling product hydrogen as (CN 1858214A) but such as Zhejiang University's a kind of reed mentioned in ancient books methods, but these method hydrogen generation efficiencies are lower.And utilize biomass or organic solid waste to carry out biology and produce hydrogen, at first biomass or organic solid waste to be degraded to by the acid hydrolysis or the method for enzymic hydrolysis and be more prone to the monose or the small organic molecule that are utilized by fermenting bacteria, produce hydrogen and organic acid by fermentation then, the organic acid that fermentation produces continues to produce hydrogen by photosynthetic bacterium again.The acid hydrolysis meeting increases produces the hydrogen cost, can cause secondary environmental pollution simultaneously.(CN101109014A) such as the Shanghai life science Zhou Zhihua of institute of the Chinese Academy of Sciences utilizes rubbish from cooking and ethanol fermentation waste water the like waste to be raw material, by dark fermentation and the hydrogen manufacturing of light fermentation lotus root connection, but to the fermented liquid behind the fermentation and hydrogen production need dilute and additional various nutrient elements so that promote photosynthetic bacterium growth and produce hydrogen, these can make again and produce the hydrogen process complications.
The 3rd, in the photosynthetic hydrogen production process, most researchs still utilize pure photosynthetic bacterium to carry out photosynthetic hydrogen production.But because the organic acid composition more complicated behind the fermentation and hydrogen production, single pure photosynthetic bacterium utilizes the ability difference very big to different organic acids, so photosynthetic hydrogen production efficient is very low.
Therefore, produce problem and the defective that exists in the hydrogen process, the present invention proposes a kind of product hydrogen methods that product hydrogen technology is more simplified, hydrogen generation efficiency is higher that utilizes natural biological materials such as Ipomoea batatas, Chinese yam at present fermentation-photosynthetic coupling.
Ipomoea batatas is one of staple food crop of China, and China only is confined to extract its starch for the application of Ipomoea batatas at present, reports for utilizing Ipomoea batatas to generate the existing part of ethanol, but does not also utilize Ipomoea batatas to generate the report of hydrogen.
Rhizome of Peltate Yam (Dioscorea Zingiberensis C.H Wright) is the perennial voluble herb plant of Dioscoreaceae Wild yam, be commonly called as yellow ginger, the duration and degree of heating root, it is the main plant that China extracts steroid hormone class medicine material diosgenin (diosgenin), the traditional acid hydrolyzation of domestic most employing is when decompositing diosgenin, starch in the Rhizome of Peltate Yam and Mierocrystalline cellulose do not add utilization and have just entered in the waste water, so not only wasted a large amount of resources, and the wastewater treatment difficulty is strengthened, environmental pollution is serious.If can be that raw material ferments-the photosynthetic product hydrogen of uniting with starch in the Rhizome of Peltate Yam and Mierocrystalline cellulose earlier, extract saponin again, not only help to reduce the difficulty of wastewater treatment, but also can increase economic benefit, realize the Sustainable development of Chinese yam industry.
Summary of the invention
The objective of the invention is provides a kind of method of utilizing biomass ferment-photosynthetic lotus root coproduction hydrogen such as Ipomoea batatas, Chinese yam in order to overcome the deficiencies in the prior art.
For achieving the above object, the technical solution used in the present invention is:
Biomass thermals such as the Ipomoea batatas after (1) will be with pigment broken, Chinese yam are handled, and 60～120 ℃ of temperature make it be hydrolyzed to starch and other macromole contain sugared organism;
(2) organic mixture after the said hydrolyzed is added in the product hydrogen substratum, carry out fermentation and hydrogen production under the effect of fermentative hydrogen-producing bacteria, produce a large amount of hydrogen, controlled temperature is 25～40 ℃ in the product H-H reaction, and pH 5.5～7.5, produce 2～3 days hydrogen time;
(3) with inoculating photosynthetic bacterium behind the adjusting of the fermented liquid behind the above-mentioned fermentation and hydrogen production pH, realize fermenting bacteria and photosynthetic bacterium lotus root coproduction hydrogen, controlled temperature is 20～40 ℃ in the reaction, and pH 6.0～7.5, and intensity of illumination 2000～8000lux produces 5～8 days hydrogen time.
(4) add biomass such as Ipomoea batatas, Chinese yam again after the above-mentioned steps photosynthetic hydrogen production is finished, carry out Secondary Fermentation and produce hydrogen; Fermented liquid carries out photosynthetic lotus root coproduction hydrogen after regulating pH once more, and fermented liquid can reuse 3-5 time.
(5) after fermented liquid reuses through 3-5 time in the above-mentioned steps, in reactor, add fermention medium, carry out new round circulation.Unnecessary photosynthetic thalline behind the photosynthetic hydrogen production also can be made photosynthetic preparation.
Further specify as of the present invention, fermentation-photosynthetic lotus root coproduction hydrogen carries out in same reactor.Be used to ferment-the natural biological material of photosynthetic lotus root coproduction hydrogen can also be the root of kudzu vine, cassava, potato etc.
Further specify as of the present invention, the product hydrogen medium component in the described step (2) is: FeCl
30.001～0.01g, NaMoO
47H
2O 0.002～0.02g, somatomedin 0.1～0.5g, tap water 1L.Somatomedin can be peptone, extractum carnis, wort or corn steep liquor.
Further specify as of the present invention, its concentration of substrate is 1～10g dry weight/L when carrying out fermentation and hydrogen production after biomass thermals such as Ipomoea batatas, Chinese yam are handled in the described step (2); The mixed fermentation hydrogen-producing bacteria is conventional bacterial classifications such as Clostridium chartatabidum, Clostridium colicanis, Bacillus sp. and Clostridium butyricum, and the fermentation and hydrogen production inoculum size is 0.5～2%.
Further specify as of the present invention, photosynthetic bacteria group comprises Rhodobacter sphaeroides, Rhodopseudomonas faecalis and Rhodopseudomonas palustris described in the step (3), and the photosynthetic hydrogen production inoculum size is 3～5%.
Further specify as of the present invention, the growth medium of photosynthetic bacteria group is in the described step (3): sodium succinate 9.8g, yeast extract paste 1g, (NH
4)
2SO
41.25g, KH
2PO
40.5g, K
2HPO
40.6g, NaCl 0.2g, MgSO
47H
2O 0.6g, CaCl
20.05g, FeSO
47H
2O 1mg, (NH
4)
6Mo
7O
240.5mg, CoCl
26H
2O 0.01mg, ZnCl
20.1mg, CuCl
20.01mg, H
3BO
32mg, EDTA-2Na 2mg, vitamins B
11mg, vitamin H 15 μ g, distilled water 1L.
Compared with prior art, the present invention has following advantage:
At first, the present invention utilizes originate widely natural biomass Ipomoea batatas, Chinese yam etc. to ferment-photosynthetic lotus root coproduction hydrogen as substrate, does not need conventional acid hydrolysis or enzymic hydrolysis process, only needs thermal treatment just can carry out fermentation and hydrogen production; Fermention medium can utilize the tap water preparation, except adding a small amount of trace element and somatomedin, does not need to add more nutrition, and it is lower that it produces the hydrogen cost, more convenient operation.
Secondly, fermentation and hydrogen production of the present invention and photosynthetic hydrogen production can successively carry out in same reactor, that fermented liquid behind the fermentation and hydrogen production does not need is centrifugal, filter, dilute or add any promotion photosynthetic bacterium growth and produce the nutritive substance of hydrogen, just can carry out photosynthetic hydrogen production by the direct inoculation photosynthetic bacterium after only need regulating pH, can save cost, simplify and produce hydrogen technology.
The 3rd, fermented liquid behind the fermentation-photosynthetic lotus root coproduction hydrogen need not be discharged first, only need add biomass such as Ipomoea batatas, Chinese yam again, fermented liquid can be used as the fermention medium recycling, realize that fermentation-photosynthetic lotus root connection circulates and produces hydrogen 3-5 time, the substrate of so not only can fully degrading carries out biology and produces hydrogen, improves substrate utilization ratio, and can save cost, reach the purpose of cleaner production.
The 4th, all adopt mixed bacterial to carry out biology among the present invention in fermentation and the photosynthetic hydrogen production process and produce hydrogen, can give full play to mixed bacterial utilize the substrate wide ranges, to environmental resistance by force, work in coordination with the characteristics that hydrogen production potential is strong and hydrogen generation efficiency is high; In addition, remaining photosynthetic hydrogen production thalline also can be used as photosynthetic preparation behind the fermentation-photosynthetic lotus root coproduction hydrogen, is widely used in agricultural, herding, culture fishery.
The 5th, the starch among the present invention in the Chinese yam transforms by fermentation, and organism is fully used, and not only can produce a large amount of hydrogen, can also improve saponin yield as the pretreatment technology of saponin extraction, reduces the environmental pollution that acid hydrolysis causes.
Specific embodiments
Embodiment 1: utilize Ipomoea batatas fermentation-photosynthetic lotus root coproduction hydrogen
1. Ipomoea batatas thermal treatment: Ipomoea batatas is broken with pigment, and substrate is to handle 20 minutes in 110 ℃ after the hydrogen substratum is produced in 5g (dry weight)/L, interpolation.Producing the hydrogen medium component is: FeCl
30.005g, NaMoO
47H
2O 0.01g, yeast extract paste 0.3g, tap water 1L.
2. fermentation and hydrogen production: the mixed fermentation hydrogen-producing bacteria is seeded in the above-mentioned heat treated Ipomoea batatas nutrient solution by 2% inoculum size carries out hydrogen production through anaerobic fermentation, fermentating liquid volume is 100mL, is reflected in the 300mL vial to carry out, and the coefficient of promptly feeding is 0.33.Controlled temperature is 35 ℃, and initial pH is 7.0, leaves standstill the normal pressure bottom fermentation and produces hydrogen.
3. produce the hydrogen acid process: behind the 6h, fermenting bacteria begins to produce hydrogen, whole product hydrogen process continues 48～72 hours, hydrogen output is 279ml/g (dry weight), maximum hydrogen-producing speed is 110.85ml/l/h, density of hydrogen is 37.84%, and producing the hydrogen rate according to contained total reducing sugar (86%) conversion in the 1g dry weight in the Ipomoea batatas is 2.35mol-H
2/ mol-glucose.
4. photosynthetic bacteria group utilizes the fermented liquid behind the fermentation and hydrogen production to carry out photosynthetic hydrogen production:
It is 7.0 back inoculation photosynthetic bacteria groups that fermented liquid behind the fermentation and hydrogen production (need not add any other nutritive substance) is regulated pH, and inoculum size is 5% (v/v).Controlled temperature is 30 ℃ in the culturing process, initial pH 7, and intensity of illumination 4000lux leaves standstill photosynthetic hydrogen production under the normal pressure.The photosynthetic hydrogen production time is about 7 days, and hydrogen output is 785ml/g (an Ipomoea batatas dry weight), and maximum hydrogen-producing speed is 23.35ml/l/h, and producing the hydrogen rate according to contained total reducing sugar (86%) conversion in the 1g dry weight in the Ipomoea batatas is 6.61mol-H
2/ mol-glucose.
Fermentation-photosynthetic lotus root connection hydrogen output is 1064ml/g (dry weight), and producing the hydrogen rate according to contained total reducing sugar (86%) conversion in the 1g dry weight in the Ipomoea batatas is 8.96mol-H
2/ mol-glucose has improved 2.8 times than independent hydrogen production through anaerobic fermentation.
Embodiment 2: differing temps bottom fermentation-photosynthetic lotus root coproduction hydrogen
Method steps is with embodiment 1, and just the fermenting process controlled temperature is at 30 ℃.The fermentation and hydrogen production process has continued 66 hours, and hydrogen output is 248ml/g (dry weight), and hydrogen-producing speed is 23ml/l/h, and producing the hydrogen rate according to contained total reducing sugar (86%) conversion in the 1g dry weight in the Ipomoea batatas is 2.09mol-H
2/ mol-glucose; Behind the fermented liquid inoculation hydrogen production with photosynthetic bacteria behind the Ipomoea batatas fermentation and hydrogen production, the photosynthetic hydrogen production amount is 556ml/g (dry weight), and hydrogen-producing speed is 19.40ml/l/h, and it is 4.68mol-H that the hydrogen rate is produced in conversion
2/ mol-glucose.Fermentation and photosynthetic lotus root connection hydrogen output be 804ml/g (dry weight) altogether, and converting its product hydrogen rate is 6.77mol-H
2/ mol-glucose has improved 2.2 times than independent hydrogen production through anaerobic fermentation.The two step lotus root connection hydrogen outputs that 30 ℃ of ratios of leavening temperature are 35 ℃ have reduced about 20%.
Embodiment 3: the fermentation of different coefficients-photosynthetic lotus root coproduction hydrogen
Method steps is with embodiment 1, and fermentating liquid volume is 150mL, and fermentation is still carried out in the 300mL vial with the photosynthetic product hydrogen of uniting, and the coefficient of promptly feeding is 0.5.The fermentation and hydrogen production amount is the 225ml/g dry weight, and hydrogen-producing speed is 22.8ml/l/h, and density of hydrogen is 50.2%, has exceeded 12.36% than embodiment 1, and producing the hydrogen rate according to contained total reducing sugar (86%) conversion in the 1g dry weight in the Ipomoea batatas is 1.89mol-H
2/ mol-glucose, hydrogen output has reduced by 20% than embodiment 1; Behind the fermented liquid inoculation hydrogen production with photosynthetic bacteria behind the Ipomoea batatas fermentation and hydrogen production, the photosynthetic hydrogen production amount is the 457ml/g dry weight, and hydrogen-producing speed is 18.2ml/l/h, and it is 3.85mol-H that the hydrogen rate is produced in conversion
2/ mol-glucose.Fermentation and photosynthetic lotus root connection hydrogen output are the 692ml/g dry weight altogether, and converting its product hydrogen rate is 5.74mol-H
2/ mol-glucose has improved 2 times than independent hydrogen production through anaerobic fermentation.
Embodiment 4: Ipomoea batatas fermentation-photosynthetic lotus root coproduction hydrogen under the different somatomedins
Method steps is with embodiment 1, and somatomedin is the 0.3g/L corn steep liquor.The fermentation and hydrogen production amount is the 279ml/g dry weight, and hydrogen-producing speed is 42.9ml/l/h, and producing the hydrogen rate according to contained total reducing sugar (86%) conversion in the 1g dry weight in the Ipomoea batatas is 2.35mol-H
2/ mol-glucose; After fermented liquid inoculation photosynthetic bacteria group behind the Ipomoea batatas fermentation and hydrogen production produced hydrogen, the photosynthetic hydrogen production amount was the 686ml/g dry weight, and hydrogen-producing speed is 16.7ml/l/h, and it is 5.77mol-H that the hydrogen rate is produced in conversion
2/ mol-glucose.Fermentation and photosynthetic lotus root connection hydrogen output are the 965ml/g dry weight altogether, and converting its product hydrogen rate is 8.12mol-H
2/ mol-glucose has improved 2.5 times than independent hydrogen production through anaerobic fermentation.
Embodiment 5: the repeatedly lotus root coproduction hydrogen that utilizes Ipomoea batatas photosynthetic hydrogen production fermented liquid
Method steps is with embodiment 1.The fermentation and hydrogen production amount is 237ml/g (dry weight), and converting its product hydrogen rate is 2.00mol-H
2/ mol-glucose; After fermented liquid inoculation photosynthetic bacteria group behind the Ipomoea batatas fermentation and hydrogen production produced hydrogen, the photosynthetic hydrogen production amount was 435ml/g (dry weight), and converting its product hydrogen rate is 3.66mol-H
2/ mol-glucose.Fermentation and photosynthetic lotus root connection hydrogen output are 672ml/g (dry weight) altogether, and converting its product hydrogen rate is 5.66mol-H
2/ mol-glucose.
Fermented liquid adjusting pH behind light and the product hydrogen is 7.0 for the first time, and adding concentration of substrate is the Ipomoea batatas of 5g (dry weight)/L, carries out the fermentation and hydrogen production second time by fermenting bacteria; Its hydrogen output is 299ml/g (dry weight) in the fermenting process, and converting its product hydrogen rate is 2.52mol-H
2/ mol-glucose; Behind the fermentation and hydrogen production, the pH that regulates Secondary Fermentation liquid once more is 7.0, utilizes the photosynthetic bacterium in the reactor to carry out secondary light and produce hydrogen, and the photosynthetic hydrogen production amount is 416ml/g (dry weight), and converting its product hydrogen rate is 3.50mol-H
2/ mol-glucose.Fermentation-photosynthetic lotus root di-time hydrogen output is 715ml/g (dry weight) altogether, and converting its product hydrogen rate is 6.02mol-H
2/ mol-glucose is than having improved for the first time 0.36mol-H
2/ mol-glucose.
Continue to utilize the fermented liquid behind the above-mentioned secondary lotus root coproduction hydrogen to ferment for the third time-photosynthetic lotus root coproduction hydrogen, operating process is the same, and the fermentation and hydrogen production amount is 246ml/g (dry weight) for the third time, and converting it, to produce hydrogen rate be 2.07mol-H
2/ mol-glucose; The photosynthetic hydrogen production amount is 490ml/g (dry weight), and converting its product hydrogen rate is 4.13mol-H
2/ mol-glucose.Fermentation and photosynthetic lotus root connection hydrogen output are 736ml/g (dry weight) altogether, and converting its product hydrogen rate is 6.20mol-H
2/ mol-glucose is than having improved for the first time 0.54mol-H
2/ mol-glucose.
Comprehensive three fermentation-photosynthetic lotus root coproduction hydrogen, its average fermentation hydrogen output is 261ml/g (dry weight), converting its product hydrogen rate is 2.19mol-H
2/ mol-glucose; Average photosynthetic hydrogen output is 447ml/g (dry weight), and converting its product hydrogen rate is 3.76mol-H
2/ mol-glucose.Fermentation and photosynthetic associating hydrogen output average out to 736ml/g (dry weight), converting its product hydrogen rate is 5.95mol-H
2/ mol-glucose is than having improved for the first time 0.29mol-H
2/ mol-glucose.
Embodiment 6: utilize Chinese yam fermentation-photosynthetic lotus root coproduction hydrogen
Method steps is with embodiment 1, and somatomedin is the 0.5g/L yeast extract paste.The fermentation and hydrogen production amount is 266ml/g (dry weight), and maximum hydrogen-producing speed is 33.2ml/l/h, and producing the hydrogen rate according to contained total reducing sugar (68%) conversion in the 1g dry weight in the Chinese yam is 2.55mol-H
2/ mol-glucose, butyric acid is 1～3g/L in the produced simultaneously organic acid of fermentation and hydrogen production, acetate 0.7～1.7g/L also has a spot of ethanol and propionic acid.After fermented liquid inoculation photosynthetic bacteria group behind the fermentation and hydrogen production produced hydrogen, the photosynthetic hydrogen production amount was 738ml/g (dry weight), and maximum hydrogen-producing speed is 44.1ml./l/h, and fermentation and photosynthetic lotus root connection hydrogen output are the 1004ml/g dry weight altogether, and converting its product hydrogen rate is 9.64mol-H
2/ mol-glucose has improved 3.8 times than independent hydrogen production through anaerobic fermentation.Product hydrogen is finished secondary fermentation liquid can also be used for the saponin extraction.
Embodiment 7: the different Chinese yam concentration fermentation-photosynthetic product hydrogen of uniting
Mode is with embodiment 1, and the Chinese yam substrate is 7g (dry weight)/L, and behind the fermentation and hydrogen production 48h, fermentation and hydrogen production amount, maximum hydrogen-producing speed and product hydrogen rate are respectively 246.9ml/g (dry weight), 34.66ml/l/h and 2.37mol-H
2/ mol-glucose; After fermented liquid inoculation photosynthetic bacteria group behind the fermentation and hydrogen production produced hydrogen, its photosynthetic hydrogen production amount was 322ml/g (dry weight), and maximum hydrogen-producing speed is 15.2ml/l/h, and the photosynthetic associating hydrogen output that comprehensively ferments is 569ml/g (dry weight), and converting its product hydrogen rate is 5.46mol-H
2/ mol-glucose.Compare with example 1, the Chinese yam substrate is that 7g (dry weight)/L is the product hydrogen total amount of 5g (dry weight)/L than the Chinese yam substrate and produces the hydrogen rate and all reduced more than 30%.After product hydrogen is finished fermented liquid being used for saponin extracts.
Using such method, utilize final fermented liquid behind biomass ferment-photosynthetic lotus root coproduction hydrogen such as Ipomoea batatas, Chinese yam can finish 3～5 times and produce hydrogen, this for the utilization ratio that improves biomass substrates such as Ipomoea batatas, Chinese yam, increase fermentation-photosynthetic lotus root coproduction hydrogen efficiency, reduce the optimization process of producing hydrogen cost and product hydrogen waste water very big promoter action is arranged.
In sum, the present invention has developed a kind of efficient, practical biological hydrogen production system, has realized the doulbe-sides' victory that resource circulation utilization and clean energy are produced, and has significant environmental benefit, economic benefit and social benefit, has a extensive future.In addition, the invention is not restricted to specific examples described above, variation and change that those skilled in the art make under claim prerequisite of the present invention all should be protection scope of the present invention.
Claims (10)
1. the method for biomass ferment-photosynthetic lotus root coproduction hydrogen is characterized in that: fermentation and hydrogen production and photosynthetic hydrogen production successively carry out in same reactor, may further comprise the steps:
(1) biomass thermal after will pulverizing is handled, and 60～120 ℃ of temperature make it be hydrolyzed to starch and other macromole contain sugared organism;
(2) organic mixture after the said hydrolyzed is added in the fermentation and hydrogen production substratum, controlled temperature is 25～40 ℃ in the product H-H reaction, and pH is 5.5～7.5, produces 2～3 days hydrogen time;
(3) will proceed to produce hydrogen behind the inoculation photosynthetic bacteria group behind the adjusting of the fermented liquid behind the above-mentioned fermentation and hydrogen production pH, controlled temperature is 20～40 ℃ in the reaction, and pH 6.0～7.5, and intensity of illumination 2000～8000lux produces 5～8 days hydrogen time;
(4) add biomass after photosynthetic hydrogen production is finished again, ferment once more-photosynthetic lotus root connection fermentation and hydrogen production; Substrate can reuse 3～5 times.
2. the method for a kind of biomass ferment according to claim 1-photosynthetic lotus root coproduction hydrogen is characterized in that: described biomass are Ipomoea batatas or Chinese yam or the root of kudzu vine or cassava or potato.
3. the method for a kind of biomass ferment according to claim 1-photosynthetic lotus root coproduction hydrogen is characterized in that: described fermentation and hydrogen production substratum contains FeCl
3, NaMoO
47H
2O, somatomedin, tap water 1L.
4. the method for a kind of biomass ferment according to claim 1-photosynthetic lotus root coproduction hydrogen is characterized in that: described fermentation and hydrogen production culture medium prescription is FeCl
30.001-0.01g, NaMoO
47H
2O0.002-0.02g, somatomedin 0.1～0.5g, tap water 1L.
5. according to the method for claim 3 or 4 described a kind of biomass ferments-photosynthetic lotus root coproduction hydrogen, it is characterized in that: described somatomedin is yeast extract paste or peptone or extractum carnis or wort or corn steep liquor.
6. the method for a kind of biomass ferment according to claim 1-photosynthetic lotus root coproduction hydrogen is characterized in that: its concentration of substrate is 1～10g (dry weight)/L when carrying out fermentation and hydrogen production after biomass thermal is handled.
7. the method for a kind of biomass ferment according to claim 1-photosynthetic lotus root coproduction hydrogen is characterized in that: fermentative hydrogen-producing bacteria is Clostridium chartatabidum, Clostridium colicanis., Bacillus sp. and Clostridium butyricum.
8. the method for a kind of biomass ferment according to claim 1-photosynthetic lotus root coproduction hydrogen is characterized in that: photosynthetic bacteria group is Rhodobacter sphaeroides, Rhodopseudomonas faecalis and Rhodopseudomonas palustris.
9. the method for a kind of biomass ferment according to claim 8-photosynthetic lotus root coproduction hydrogen, it is characterized in that: the growth medium of described photosynthetic bacteria group contains: sodium succinate, yeast extract paste, (NH
4)
2SO
4, KH
2PO
4, K
2HPO
4, NaCl, MgSO
47H
2O, CaCl
2, FeSO
47H
2O, (NH
4)
6Mo
7O
24, CoCl
26H
2O, ZnCl
2, CuCl
2, H
3BO
3, EDTA-2Na, vitamins B
1, vitamin H, distilled water.
10. the method for a kind of biomass ferment according to claim 8-photosynthetic lotus root coproduction hydrogen is characterized in that: the grown cultures based formulas of described photosynthetic bacteria group is: sodium succinate 9.8g, yeast extract paste 1g, (NH
4)
2SO
41.25g, KH
2PO
40.5g, K
2HPO
40.6g, NaCl 0.2g, MgSO
47H
2O 0.6g, CaCl
20.05g, FeSO
47H
2O 1mg, (NH
4)
6Mo
7O
240.5mg, CoCl
26H
2O 0.01mg, ZnCl
20.1mg, CuCl
20.01mg, H
3BO
32mg, EDTA-2Na 2mg, vitamins B
11mg, vitamin H 15 μ g, distilled water 1L.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102370147A (en) * | 2010-08-04 | 2012-03-14 | 浙江高山农业发展有限公司 | Method for processing and producing pickle products by circularly utilizing bifida ferment lysate |
| CN102876724A (en) * | 2012-09-28 | 2013-01-16 | 重庆大学 | Coupling method based on synchronous cellulose enzymolysis and fermentation and microbial electrolysis cell hydrogen production |
| CN110157612A (en) * | 2019-05-10 | 2019-08-23 | 河南农业大学 | A kind of photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor and the method using its progress hydrogen manufacturing |
-
2008
- 2008-12-25 CN CN2008101480301A patent/CN101760479B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102370147A (en) * | 2010-08-04 | 2012-03-14 | 浙江高山农业发展有限公司 | Method for processing and producing pickle products by circularly utilizing bifida ferment lysate |
| CN102876724A (en) * | 2012-09-28 | 2013-01-16 | 重庆大学 | Coupling method based on synchronous cellulose enzymolysis and fermentation and microbial electrolysis cell hydrogen production |
| CN102876724B (en) * | 2012-09-28 | 2014-05-28 | 重庆大学 | Coupling method based on synchronous cellulose enzymolysis and fermentation and microbial electrolysis cell hydrogen production |
| CN110157612A (en) * | 2019-05-10 | 2019-08-23 | 河南农业大学 | A kind of photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor and the method using its progress hydrogen manufacturing |
| CN110157612B (en) * | 2019-05-10 | 2022-09-27 | 河南农业大学 | Photosynthetic bacteria culture-light fermentation hydrogen production combined reactor and method for producing hydrogen by using same |
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| Publication number | Publication date |
|---|---|
| CN101760479B (en) | 2012-01-04 |
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