CN110157199A - A kind of alginate gelatin hydrogel preparation method - Google Patents
A kind of alginate gelatin hydrogel preparation method Download PDFInfo
- Publication number
- CN110157199A CN110157199A CN201910414113.9A CN201910414113A CN110157199A CN 110157199 A CN110157199 A CN 110157199A CN 201910414113 A CN201910414113 A CN 201910414113A CN 110157199 A CN110157199 A CN 110157199A
- Authority
- CN
- China
- Prior art keywords
- solution
- alginate
- gelatin
- phosphate
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108010010803 Gelatin Proteins 0.000 title claims abstract description 53
- 239000008273 gelatin Substances 0.000 title claims abstract description 53
- 229920000159 gelatin Polymers 0.000 title claims abstract description 53
- 235000019322 gelatine Nutrition 0.000 title claims abstract description 53
- 235000011852 gelatine desserts Nutrition 0.000 title claims abstract description 53
- 235000010443 alginic acid Nutrition 0.000 title claims abstract description 28
- 229920000615 alginic acid Polymers 0.000 title claims abstract description 28
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 229940072056 alginate Drugs 0.000 title claims abstract description 27
- 239000000017 hydrogel Substances 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 239000000243 solution Substances 0.000 claims abstract description 46
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000000661 sodium alginate Substances 0.000 claims abstract description 20
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 20
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 20
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 18
- 239000010452 phosphate Substances 0.000 claims abstract description 18
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 12
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 claims abstract description 11
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims abstract description 9
- 239000002953 phosphate buffered saline Substances 0.000 claims abstract description 9
- 150000001718 carbodiimides Chemical class 0.000 claims abstract description 8
- 239000008367 deionised water Substances 0.000 claims abstract description 8
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 6
- 238000006243 chemical reaction Methods 0.000 claims abstract description 5
- 239000000499 gel Substances 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 238000004026 adhesive bonding Methods 0.000 claims abstract description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 claims description 8
- 230000003647 oxidation Effects 0.000 claims description 6
- 238000007254 oxidation reaction Methods 0.000 claims description 6
- 230000004048 modification Effects 0.000 claims description 5
- 238000012986 modification Methods 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000005485 electric heating Methods 0.000 claims description 3
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 3
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 3
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 229910052698 phosphorus Inorganic materials 0.000 claims description 2
- 239000011574 phosphorus Substances 0.000 claims description 2
- 238000009938 salting Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims 3
- 239000003292 glue Substances 0.000 claims 2
- 239000000783 alginic acid Substances 0.000 claims 1
- 229960001126 alginic acid Drugs 0.000 claims 1
- 150000004781 alginic acids Chemical class 0.000 claims 1
- 238000002242 deionisation method Methods 0.000 claims 1
- 238000000502 dialysis Methods 0.000 claims 1
- 239000012266 salt solution Substances 0.000 claims 1
- 241001474374 Blennius Species 0.000 abstract description 5
- 230000015556 catabolic process Effects 0.000 abstract description 5
- 238000006731 degradation reaction Methods 0.000 abstract description 5
- 150000003839 salts Chemical class 0.000 abstract description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract description 2
- 239000011734 sodium Substances 0.000 abstract description 2
- 229910052708 sodium Inorganic materials 0.000 abstract description 2
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 abstract 1
- 238000013019 agitation Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003513 alkali Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- ANOBYBYXJXCGBS-UHFFFAOYSA-L stannous fluoride Chemical compound F[Sn]F ANOBYBYXJXCGBS-UHFFFAOYSA-L 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H1/00—Macromolecular products derived from proteins
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/04—Alginic acid; Derivatives thereof
Abstract
The invention discloses a kind of alginate gelatin hydrogel preparation methods, the raw material for preparing hydrogel includes: gelatin, ethylenediamine, concentrated hydrochloric acid, carbodiimide, phosphate solution, oxidized sodium alginate, phosphate buffered saline solution, deionized water, described to prepare water-setting gluing method as follows: gelatin passes through phosphate, ethylenediamine carries out reacting obtained modified gelatin, quantitative oxidized sodium alginate and phosphate buffered saline solution are mixed to get alginate solution, it takes a certain amount of modified gelatin to be dissolved in deionized water again and obtains modified gelatin solution, then the modified gelatin solution of 10% alginate solution and 20% is mixed after being stirred 15s, it is pushed it into tissue culture plate using double syringe, it is placed in gel reaction 10min in 37 DEG C of insulating boxs, the present invention is to pass through periodic acid by oxidized sodium alginate Sodium is modified, so that oxidized sodium alginate has good degradation property, to solve the problems, such as that the degradation speed of previous seaweed salt itself is slow.
Description
Technical field
The present invention relates to hydrogel field more particularly to a kind of alginate gelatin hydrogel preparation methods.
Background technique
Sodium alginate and gelatin are natural biologic materials, because its is cheap, safe and reliable and biocompatibility is good,
It is widely used in organizational project and related fields, however the degradation speed of seaweed salt itself is slow, and hydrogel is water-soluble
Difference, these defects limit application of the seaweed salt in organizational project, and the property of easy gel is hydrogel under gelatin room temperature
Operation in preparation process brings certain trouble, therefore, it is proposed that a kind of alginate gelatin hydrogel preparation method.
Summary of the invention
The purpose of the present invention is to provide a kind of alginate gelatin hydrogel preparation method, solves institute in background technique
The problem of proposition.
To achieve the above object, the invention provides the following technical scheme: a kind of alginate gelatin hydrogel preparation method,
The raw material for preparing hydrogel includes: gelatin, ethylenediamine, concentrated hydrochloric acid, carbodiimide, phosphate solution, oxidation seaweed
Sour sodium, phosphate buffered saline solution, deionized water, described to prepare water-setting gluing method as follows: gelatin is carried out by phosphate, ethylenediamine
It reacts and modified gelatin is made, quantitative oxidized sodium alginate and phosphate buffered saline solution are mixed to get alginate solution, then
It takes a certain amount of modified gelatin to be dissolved in deionized water and obtains modified gelatin solution, then by 10% alginate solution and 20%
After the mixing of modified gelatin solution is stirred 15s, is pushed it into tissue culture plate using double syringe, be placed in 37 DEG C of constant temperature
Gel reaction 10min in case.
As a kind of preferred embodiment of the invention, the modified gelatin the preparation method is as follows: by 5g Gelatin in
In 0.1M phosphate solution, after being completely dissolved, 14g ethylenediamine is added, is carried out using magnetic agitation uniformly mixed, the dense of 6M is added
Then to 5 2.675 carbodiimides are added, then be settled to 250ml with phosphate solution, at 37 DEG C in salt acid for adjusting pH value
1h is reacted in insulating box, is cooled to room temperature, product is fitted into bag filter, is dialysed 3 days, is changed daily water 4 times, by it after having dialysed
It imports in frosting ware, is put into low temperature refrigerator pre-freeze for 24 hours, then be dried.
As a kind of preferred embodiment of the invention, the alginate solution is the preparation method is as follows: by quantitative oxygen
Then the phosphate buffered saline solution that change sodium alginate is dissolved in pH most 7.4 places it in 37 using magnetic agitation to being completely dissolved
DEG C insulating box in place 0.5h.
As a kind of preferred embodiment of the invention, the modified gelatin solution manufacturing method is as follows: quantitative is changed
Property gelatin is dissolved in deionized water, is prepared certain density solution, is first placed it in 50 DEG C of electric heating constant-temperature blowing drying box,
It is completely dissolved to it, goes in 37 DEG C of insulating box and place 0.5h.
As a kind of preferred embodiment of the invention, the phosphate solution passes through sodium dihydrogen phosphate and sodium hydroxide tune
Be made, and the pH value of phosphate solution be 5.
As a kind of preferred embodiment of the invention, the oxidized sodium alginate by sodium metaperiodate to sodium alginate into
Obtained by row oxidation modification.
Compared with prior art, beneficial effects of the present invention are as follows:
1. alginate gelatin hydrogel preparation method of the invention, by oxidized sodium alginate is changed by sodium metaperiodate
Property, so that oxidized sodium alginate has good degradation property, so that the degradation speed for solving previous seaweed salt itself is slow
The problem of.
2. alginate gelatin hydrogel preparation method of the invention utilizes ethylenediamine by the catalysis of carbodiimide
Gelatin is improved, so that the part carboxyl in original gelatin is changed into amino, modified gelatin viscosity is low, water-soluble
Improve to effective.
3. alginate gelatin hydrogel preparation method of the invention, by modified gelatin and oxidized sodium alginate in room temperature
Lower reaction can form the covalent hydrogel of alkali structure, so as to reduce the potential physiological-toxicity of aldehyde radical.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to
Specific embodiment, the present invention is further explained.
A kind of alginate gelatin hydrogel preparation method, the raw material for preparing hydrogel include: gelatin, ethylenediamine,
Concentrated hydrochloric acid, carbodiimide, phosphate solution, oxidized sodium alginate, phosphate buffered saline solution, deionized water.
Preparation method: 1, modified gelatin is completely molten the preparation method is as follows: by 5g Gelatin in 0.1M phosphate solution
14g ethylenediamine is added in Xie Hou, is carried out using magnetic agitation uniformly mixed, and the concentrated hydrochloric acid that 6M is added adjusts pH value to 5, then plus
Enter 2.675 carbodiimides, then be settled to 250ml with phosphate solution, reacts 1h in 37 DEG C of insulating box, be cooled to room
Product is fitted into bag filter by temperature, is dialysed 3 days, is changed water 4 times daily, be conducted into frosting ware, be put into low after having dialysed
Temperature refrigerator pre-freeze for 24 hours, then is dried.
2, quantitative oxidized sodium alginate the preparation method is as follows: is dissolved in the phosphorus of pH most 7.4 by the alginate solution
Then acid buffering salting liquid places it in 37 DEG C of insulating box using magnetic agitation to being completely dissolved and places 0.5h.
3, the modified gelatin solution manufacturing method is as follows: quantitative modified gelatin being dissolved in deionized water, prepares one
Determine the solution of concentration, first place it in 50 DEG C of electric heating constant-temperature blowing drying box, be completely dissolved to it, goes to 37 DEG C of constant temperature
0.5h is placed in case
4,10% alginate solution and 20% modified gelatin solution mixing be stirred 15s after, utilize double syringe will
It is pushed into tissue culture plate, is placed in gel reaction 10min in 37 DEG C of insulating boxs and alginate gelatin hydrogel is made.
Wherein, the phosphate solution is made by sodium dihydrogen phosphate and sodium hydroxide allotment, and the pH of phosphate solution
Value is 5, and the oxidized sodium alginate carries out obtained by oxidation modification sodium alginate as sodium metaperiodate.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of alginate gelatin hydrogel preparation method, it is characterised in that: the raw material for preparing hydrogel includes: bright
Glue, ethylenediamine, concentrated hydrochloric acid, carbodiimide, phosphate solution, oxidized sodium alginate, phosphate buffered saline solution, deionization
Water, described to prepare water-setting gluing method as follows: gelatin carries out reacting obtained modified gelatin by phosphate, ethylenediamine, will be quantitative
Oxidized sodium alginate and phosphate buffered saline solution are mixed to get alginate solution, then take a certain amount of modified gelatin be dissolved in from
Sub- water obtains modified gelatin solution, then mixes 10% oxidation alginate solution and 20% modified gelatin solution and carries out
After stirring 15s, is pushed it into tissue culture plate using double syringe, be placed in gel reaction 10min in 37 DEG C of insulating boxs.
2. a kind of alginate gelatin hydrogel preparation method according to claim 1, it is characterised in that: the modification is bright
Glue after being completely dissolved, is added 14g ethylenediamine, utilizes magnetic the preparation method is as follows: by 5g Gelatin in 0.1M phosphate solution
Power stirring carry out it is uniformly mixed, be added 6M concentrated hydrochloric acid adjust pH value to 5,2.675 carbodiimides are then added, then use phosphorus
Acid salt solution is settled to 250ml, reacts 1h in 37 DEG C of insulating box, is cooled to room temperature, product is fitted into bag filter, dialysis
It 3 days, changes daily water 4 times, is conducted into after having dialysed in frosting ware, is put into low temperature refrigerator pre-freeze for 24 hours, then be dried i.e.
It can.
3. a kind of alginate gelatin hydrogel preparation method according to claim 1, it is characterised in that: the alginic acid
Salting liquid utilizes magnetic force the preparation method is as follows: quantitative oxidized sodium alginate to be dissolved in the phosphate buffered saline solution of pH most 7.4
Then stirring places it in 37 DEG C of insulating box to being completely dissolved and places 0.5h.
4. a kind of alginate gelatin hydrogel preparation method according to claim 1, it is characterised in that: the modification is bright
Sol solution is prepared certain density solution, is first set the preparation method is as follows: quantitative modified gelatin is dissolved in deionized water
It in 50 DEG C of electric heating constant-temperature blowing drying box, is completely dissolved to it, goes in 37 DEG C of insulating box and place 0.5h.
5. a kind of alginate gelatin hydrogel preparation method according to claim 1, it is characterised in that: the phosphate
Solution is made by sodium dihydrogen phosphate and sodium hydroxide allotment, and the pH value of phosphate solution is 5.
6. a kind of alginate gelatin hydrogel preparation method according to claim 1, it is characterised in that: the oxidation sea
Mosanom carries out obtained by oxidation modification sodium alginate as sodium metaperiodate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910414113.9A CN110157199A (en) | 2019-05-17 | 2019-05-17 | A kind of alginate gelatin hydrogel preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910414113.9A CN110157199A (en) | 2019-05-17 | 2019-05-17 | A kind of alginate gelatin hydrogel preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110157199A true CN110157199A (en) | 2019-08-23 |
Family
ID=67631142
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910414113.9A Pending CN110157199A (en) | 2019-05-17 | 2019-05-17 | A kind of alginate gelatin hydrogel preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110157199A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101716366A (en) * | 2009-12-17 | 2010-06-02 | 天津大学 | Biocolloid hemostatic prepared by aldehyde-modified sodium alginate and amine-modified gelatine |
| CN107050510A (en) * | 2017-06-14 | 2017-08-18 | 东华大学 | A kind of sodium alginate/glutin injectable double-network hydrogel and its preparation and application |
| CN108586829A (en) * | 2018-05-18 | 2018-09-28 | 北京化工大学 | A kind of preparation method of conduction self-healing hydrogel and conductive self-healing circuit |
-
2019
- 2019-05-17 CN CN201910414113.9A patent/CN110157199A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101716366A (en) * | 2009-12-17 | 2010-06-02 | 天津大学 | Biocolloid hemostatic prepared by aldehyde-modified sodium alginate and amine-modified gelatine |
| CN107050510A (en) * | 2017-06-14 | 2017-08-18 | 东华大学 | A kind of sodium alginate/glutin injectable double-network hydrogel and its preparation and application |
| CN108586829A (en) * | 2018-05-18 | 2018-09-28 | 北京化工大学 | A kind of preparation method of conduction self-healing hydrogel and conductive self-healing circuit |
Non-Patent Citations (1)
| Title |
|---|
| 孙言蓓: "新型海藻酸盐-明胶水凝胶的研究", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101502670B (en) | Method for preparing silk fibroin hydrogel | |
| CN107265426B (en) | template-mediated synthesis of silicon-containing hydroxyapatite material and preparation method thereof | |
| CN105295105B (en) | A kind of bacteria cellulose based composites with photocatalysis antibacterial | |
| CN103723757B (en) | Medical grade α-calcium sulphate hemihydrate preparation method | |
| CN106830746B (en) | The biological additive and preparation method thereof that concrete microcrack self-healing shares | |
| CN101297978A (en) | Preparation method of hydroxyapatite nano pole | |
| CN107661540B (en) | Method for preparing high-strength hydroxyapatite-chitosan-silicon dioxide hybrid scaffold by using 3D printing | |
| CN104906073B (en) | A kind of preparation method for the chitosan quaternary ammonium salt hyaluronic acid nanometer gel containing basic fibroblast growth factor | |
| CN104911230A (en) | Bacterial cellulose in-situ fermentation production method | |
| CN105770983B (en) | A kind of preparation method of hyaluronic acid bioadhesive | |
| CN103007342A (en) | Biodegradable and medical tricalcium phosphate/gamma-polyglutamic acid composite and preparation method thereof | |
| CN110157199A (en) | A kind of alginate gelatin hydrogel preparation method | |
| CN101294179A (en) | Method for preparing small-molecular weight hyaluronic acid by adding hyaluronate lyase in course of fermentation | |
| CN104117095B (en) | Strontium/fibroin bionic coating modifies the preparation method of artificial ligament | |
| CN115044624B (en) | Method for producing PHA by repeated batch fermentation of halomonas | |
| CN114410617B (en) | Immobilization method for improving biological hydrogen synthesis of hydrogen-producing bacteria and application | |
| CN108187136A (en) | A kind of preparation method of nanometer of load zinc hydroxyapatite | |
| CN109705230A (en) | A kind of method and carragheen for extracting carragheen from red algae | |
| CN101503492B (en) | Method for producing polyacrylamide cultivation substrate | |
| CN109650364B (en) | Micron hexagonal-column strontium-doped hydroxyapatite and synthesis process thereof | |
| CN107746115A (en) | A kind of preparation method of high flocculating rate composite flocculation agent | |
| CN101830942A (en) | Post-treatment method of flavomycin | |
| CN111908819A (en) | Liquid alkali-free accelerator and preparation method thereof | |
| CN101235096B (en) | Method for extracting polysaccharide from phormidium tenue | |
| CN105463050A (en) | Natural food additive xanthan gum and preparing process thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190823 |
|
| RJ01 | Rejection of invention patent application after publication |