CN110144344B - Double-layer movable magnetic needle and use method thereof - Google Patents
Double-layer movable magnetic needle and use method thereof Download PDFInfo
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- CN110144344B CN110144344B CN201910340427.9A CN201910340427A CN110144344B CN 110144344 B CN110144344 B CN 110144344B CN 201910340427 A CN201910340427 A CN 201910340427A CN 110144344 B CN110144344 B CN 110144344B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
The invention discloses a double-layer movable magnetic needle and a using method thereof. The double-layer movable magnetic needle comprises a magnetic needle body, wherein the magnetic needle body comprises an outer layer and an inner layer, a liquid circulation cavity is formed between the inner layer and the outer layer, one of the outer layer and the inner layer is made of a magnetic material, and the other layer is a movable piece with a sharp tail end. The double-layer movable magnetic needle provided by the invention enables the reagent in the automatic magnetic rod method to be free from uncovering, avoids pollution and is suitable for testing projects with high environmental sensitivity.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a double-layer movable magnetic needle and a using method thereof.
Background
In recent years, an automatic sample pretreatment platform based on a magnetic bead method is widely applied to nucleic acid extraction and detection. On an automated platform, a common method is a magnetic bar method, which uses a magnetic bar to absorb magnetic beads for transfer and discard liquid. The method has the advantages of high solid-liquid separation efficiency and difficult loss of magnetic beads. However, in this way the reagent must be completely uncapped for access to the magnetic rods and beads. This results in the reagent being exposed to the environment and being susceptible to contamination, and the reagent also being volatile resulting in instability of the results, thus greatly limiting the application of the magnetic rod method.
Disclosure of Invention
In order to solve the above-mentioned deficiencies of the prior art, the present invention provides a double-layer movable magnetic needle and a method for using the same.
The double-layer movable magnetic needle comprises a magnetic needle body, wherein the magnetic needle body comprises an outer layer and an inner layer, a liquid circulation cavity is formed between the inner layer and the outer layer, one of the outer layer and the inner layer is made of a magnetic material, and the other layer is a movable piece with a sharp tail end. Preferably, the inner layer is a magnetic material and the outer layer is a moving part with a sharp end.
Further, the inner layer may be hollow or solid. Preferably, the inner layer is hollow, and the hollow cavity is a liquid circulation cavity.
Further, the inner magnetic layer may be a permanent magnetic material (e.g., alnico, ferrochromoco, permanent ferrite, rare earth material, preferably neodymium iron boron).
Further, the inner magnetic portion may be a non-permanent magnetic ferrous material (e.g., iron-nickel alloy, iron-cobalt alloy, iron-aluminum alloy, iron oxide, preferably ferroferric oxide).
Further, the inner magnetic portion may externally surround the copper coil.
Further, the inner magnetic portion may be coated.
Further, the inner magnetic part can be a movable part which can move up and down or rotate.
Further, the inner magnetic part can be integrally formed or made by spraying a magnetic material on the support.
Further, the outer sharp part can be a movable part which can move up and down or rotate.
(the inner layer and the outer layer can move together or independently)
Furthermore, the sharp part of the outer layer can be provided with a part of protrusion to increase the rotary stirring function
Furthermore, the tail end of the double-layer movable magnetic needle can be connected with an electric signal to provide heating, movement and the like for the needle.
Further, a spring may be connected to a terminal (inner layer or outer layer) of the double-layer movable magnetic needle to provide a limited range of motion for the double-layer movable magnetic needle (the inner layer or outer layer).
Further, the inner layer and the outer layer can be designed integrally or separately.
Further, the inner and outer layers may be the same length, or different.
Further, the inner and outer layers may be circular or square in cross-section, preferably circular.
The invention also provides an operation method of the double-layer movable magnetic needle, which comprises the following steps:
(1) the double-layer movable magnetic needle is loaded on an automatic platform and moves to the position above a covered reagent container containing magnetic beads;
(2) the double-layer movable magnetic needle moves downwards, and the sharp part of the outer layer punctures the reagent container cover;
(3) then the inner magnetic part descends, and is electrified to generate magnetism to adsorb magnetic beads;
(4) liquid is sucked and spit through a liquid circulation cavity of the double-layer movable magnetic needle by a pump, and the magnetic bead washing is completed;
(5) the double-layer movable magnetic needle is then pulled up to leave the reagent bottle container, and the magnetic beads are separated along with the double-layer movable magnetic needle.
The double-layer movable magnetic needle has the following technical effects:
(1) the double-layer movable magnetic needle provided by the invention enables the reagent in the automatic magnetic rod method to be free from uncovering, avoids pollution and is suitable for testing projects with high environmental sensitivity.
(2) Can make some volatile reagents need not uncapping, reduce the influence of volatilizing to the result.
(3) Can be compatible with the reagent of the magnetic base method.
(4) The functions of adsorption of magnetic beads in the capping reagent, liquid sucking and spitting, stirring, heating and the like can be realized.
(5) The magnetic bead can utilize the solvent to inhale in the needle and tell the washing that realizes the magnetic bead, weathers, and the magnetic bead that moves to get is cleaner, and the result is more accurate.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed.
Drawings
The invention is illustrated by the following figures:
fig. 1 is a double-layer movable magnetic needle with a copper ring.
Fig. 2 is a double-layer movable magnetic needle with a protrusion.
Fig. 3 shows two double-layer movable magnetic needles with different shapes of the bottom sharp part.
Fig. 4 shows a cross section of a two-layer movable magnetic needle with two different structures.
Detailed Description
The objects and functions of the present invention and methods for accomplishing the same will be apparent by reference to the exemplary embodiments. However, the present invention is not limited to the exemplary embodiments disclosed below; it can be implemented in different forms. The nature of the description is merely to assist those skilled in the relevant art in a comprehensive understanding of the specific details of the invention.
The present invention will be described in detail below with reference to the accompanying drawings and examples. In the drawings, the same reference numerals denote the same or similar parts, or the same or similar steps. The present invention will be described below with reference to specific embodiments.
In one embodiment, the present invention provides a double-layered movable magnetic needle, including a magnetic needle body including an outer sharp portion and an inner magnetic portion, the inner layer being made of a magnetic material, the outer layer being a movable member having a sharp end, the outer sharp end being used to pierce a reagent container cover so that the magnetic needle body extends into the reagent container, and the inner magnetic portion being used to adsorb magnetic beads in the reagent container. A liquid circulation cavity is formed between the inner layer and the outer layer, preferably, the inner layer is hollow, and the hollow cavity is a liquid circulation cavity. And sucking and spitting the liquid through a liquid circulation cavity of the magnetic needle by a pump to complete the washing of the magnetic beads.
In the invention, the inner layer and the outer layer move together or independently, and the movement can be up and down movement or rotation. Preferably, the inner magnetic portion may be a movable member. This may be achieved by mounting the motor at the root of the inner and outer layers.
The inner magnetic layer can be made of permanent magnetic materials, such as aluminum nickel cobalt system, iron chromium cobalt system, permanent magnetic ferrite and rare earth materials, preferably neodymium iron boron; or may be a non-permanent magnetic ferrous material such as an iron-nickel alloy, an iron-cobalt alloy, an iron-aluminum alloy, an iron oxide, preferably ferroferric oxide. Preferably, the inner magnetic part may externally surround the copper coil. Preferably, the inner magnetic part may be coated.
Further, the end of the double-layer movable magnetic needle can be electrified to provide heating, movement and the like for the needle.
Further, a spring may be connected to a terminal (inner layer or outer layer) of the double-layer movable magnetic needle to provide a limited range of motion for the double-layer movable magnetic needle (the inner layer or outer layer).
Further, the inner and outer layers may be the same length or different, preferably the layer with the sharp tip is longer than the other layer.
Further, the cross-section of the inner and outer layers may be circular, square or other shapes, preferably circular.
Fig. 1 shows a schematic view of a double-layer movable magnetic needle with a copper ring. The magnetic needle comprises a needle body 101, a needle handle 102 and a flange 103 connecting the needle body 101 and the needle handle 102, wherein a spiral steel ring 104 is surrounded around the needle handle 102. As shown in fig. 2, the outer layer portion may be provided with a partial protrusion 105 to increase the rotary stirring function.
Fig. 3 shows two shapes of the bottom sharp portion, triangular and saw-toothed respectively. The triangular and saw-toothed outer sharp end is used to pierce a reagent container lid so that the magnetic needle body penetrates deep into the reagent container.
Fig. 4 shows a cross section of a two-layer movable magnetic needle with two different structures. The magnetic needle comprises a needle body comprising an outer layer 201 and an inner layer 202, wherein the inner layer 202 can be solid (figure A) or hollow (figure B). The cavity 203 between the outer layer 201 and the inner layer 202 or the hollow cavity 204 of the inner layer 202 is a liquid circulation cavity. And sucking and spitting the liquid through a liquid circulation cavity of the magnetic needle by a pump to complete the washing of the magnetic beads.
The operation mode of the double-layer movable magnetic needle of the invention is as follows: the double-layer movable magnetic needle is loaded on an automatic platform and moves to the position above a covered reagent container containing magnetic beads; the double-layer movable magnetic needle moves downwards, and the sharp part of the outer layer punctures the reagent container cover; then the inner magnetic part descends, and is electrified to generate magnetism to adsorb magnetic beads; liquid is sucked and spit through a liquid circulation cavity of the double-layer movable magnetic needle by a pump, and the magnetic bead washing is completed; the double-layer movable magnetic needle is then drawn away from the reagent container, and the magnetic beads are removed with the double-layer movable magnetic needle.
Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the invention being indicated by the following claims.
Claims (9)
1. The utility model provides a movable magnetic force needle of double-deck, includes the magnetic force needle main part, the magnetic force needle main part includes skin and inlayer, the inlayer with form the liquid circulation chamber between the skin, inlayer magnetism part is non-permanent magnetism nature iron material, the outer moving part for having sharp-pointed end, the skin is longer than the inlayer or both are isometric.
2. The double-layer movable magnetic needle according to claim 1, wherein the inner layer is hollow, and the hollow cavity is a liquid circulation cavity.
3. The double-layer movable magnetic needle according to claim 1, wherein the non-permanent magnetic ferrous material is iron-nickel alloy, iron-cobalt alloy, iron-aluminum alloy, iron oxide.
4. The double-layer movable magnetic needle of claim 1, wherein the non-permanent magnetic ferrous material is ferroferric oxide.
5. The double-layer movable magnetic needle of claim 1, wherein either or both of the outer sharp portion and the inner magnetic portion are movable members.
6. The double-layer movable magnetic needle of claim 1, wherein the outer sharp portion is partially protruded.
7. The double-layer movable magnetic needle of claim 1, wherein the end of the double-layer movable magnetic needle is energized to provide heating and movement to the needle.
8. The double-layer movable magnetic needle of claim 1, which is terminated with a spring.
9. The operating method of the double-layer movable magnetic needle according to any one of claims 1 to 8, comprising:
(1) loading the double-layer movable magnetic needle on an automatic platform, and moving the double-layer movable magnetic needle to the position above a covered reagent container containing magnetic beads;
(2) the double-layer movable magnetic needle moves downwards, and the sharp part of the outer layer punctures the reagent container cover;
(3) then the inner magnetic part descends, and is electrified to generate magnetism to adsorb magnetic beads;
(4) liquid is sucked and spit through a liquid circulation cavity of the double-layer movable magnetic needle by a pump, and the magnetic bead washing is completed;
(5) the double-layer movable magnetic needle is then pulled up to leave the reagent bottle container, and the magnetic beads are separated along with the double-layer movable magnetic needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910340427.9A CN110144344B (en) | 2019-04-25 | 2019-04-25 | Double-layer movable magnetic needle and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910340427.9A CN110144344B (en) | 2019-04-25 | 2019-04-25 | Double-layer movable magnetic needle and use method thereof |
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| CN110144344A CN110144344A (en) | 2019-08-20 |
| CN110144344B true CN110144344B (en) | 2021-01-12 |
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Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19730497C2 (en) * | 1997-07-16 | 2000-02-10 | Heermann Klaus Hinrich | Method for washing, separating and concentrating biomolecules using a magnetic pen |
| GB0517910D0 (en) * | 2005-09-05 | 2005-10-12 | Enigma Diagnostics Ltd | Liquid transfer device |
| CN103923829B (en) * | 2013-01-15 | 2015-08-26 | 常州金麦格生物技术有限公司 | The needle of bar magnet method extraction apparatus and sleeve pipe mobile system are applied with it |
| CN105219641B (en) * | 2014-05-27 | 2017-12-12 | 北京自由度科学机器有限公司 | Apparatus and method for paramagnetic particle method |
| CN206680511U (en) * | 2017-03-03 | 2017-11-28 | 安徽为臻生物工程技术有限公司 | A kind of electromagnetic type nucleic acid-extracting apparatus |
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