CN110142285A - Trichoderma asperellum-alfalfa renovation of heavy metal polluted soil with combined method - Google Patents
Trichoderma asperellum-alfalfa renovation of heavy metal polluted soil with combined method Download PDFInfo
- Publication number
- CN110142285A CN110142285A CN201910439868.4A CN201910439868A CN110142285A CN 110142285 A CN110142285 A CN 110142285A CN 201910439868 A CN201910439868 A CN 201910439868A CN 110142285 A CN110142285 A CN 110142285A
- Authority
- CN
- China
- Prior art keywords
- heavy metal
- soil
- trichoderma asperellum
- trichoderma
- restoration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Processing Of Solid Wastes (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of Trichoderma asperellum-alfalfa renovation of heavy metal polluted soil with combined methods；Contaminated soil is repaired by the heavy metal in Trichoderma asperellum and alfalfa synergistic sorption soil.It will be prepared into granule, be applied to alfalfa rhizosphere in the Trichoderma asperellum GDSF1009 that alfalfa rhizosphere colonizes.Since Trichoderma granule can stimulate lucerne perennial root system, improves it and adsorb heavy metal ability, therefore the two forms the reparation bioplex of efficient absorption heavy metal.Heavy metal adsorption efficiency in plant body reaches 51%-59%, and heavy metal in soil content reduces 58%-60%.Simultaneously by cleaning to rehabilitation plant material, recycling can be recycled.Heavy metal content in soil not only can be effectively reduced in the technology, moreover it is possible to restore soil micro-ecosystem, increase soil beneficial microbe and increase soil fertility, be an economical and effective and the plant combined rehabilitating soil new technology of the microorganism-that has a extensive future.
Description
Technical field
The invention belongs to soil remediation fields, and in particular to a kind of Trichoderma asperellum and alfalfa united repairing heavy metal
The method of contaminated soil.
Background technique
Industrial pollution, chemical fertilizer and being excessively used for pesticide cause soil pollution to get worse, it has also become serious to restrict agricultural
Production problem.In order to realize agricultural sustainable development, administering contaminated soil to push up is the great class repaired and improved the ecological environment
Topic.
Currently, mainly there are three aspects for common remediation of heavy metal-contaminated soil: peripheral doses, chemical remediation and micro-
The plant combined reparation of biological prosthetic, phytoremediation, microorganism-.Physical restoration is exactly to use certain technology and means, will be dirty
Dye object separates the method for making soil restoration use value from soil.General soil remediation physical restoration mainly has
It is several below: direct soil replacement method, the reparation of thermalization method, vitrifying restorative procedure, electrode drive repairing method.Peripheral doses are at high cost,
Poor operability, it is difficult to the soil of large area be realized and repaired.Chemical remediation is directed to add passivator in contaminated soil, makes weight
Metal is from activity to Forms Transformation is stabilized, to reduce migration and the bioavailability of heavy metal.However chemical leaching is easy
Lead to soil acidification, brokenization soil physics structure leads to the loss of soil fertility, and chemical remediation is also easy to cause underground water
The secondary pollution of equal environment.Biological prosthetic includes enriching plant absorption heavy metal and microorganism adsorption heavy metal, is mainly passed through
Generate the chelating agents curing heavy metals such as organic acid.Majority is single utilization super enriching plant, such as vetiver, centipede in the world at present
Grass, squama sedge, there are also Indian mustards (Brassica juncea) etc..Clover is green manure pulse family perennial, with fixed nitrogen function
Plant, therefore prosthetic plant is used alfalfa as, in addition to having absorption heavy metal effect, also have the function of conserving soil.
Plants enriched method is economical and practical, and can improve the ecological environment, but merely plants enriched repairs heavy-metal contaminated soil
It is difficult to reach very high efficiency again.The microorganism of rehabilitating soil is mainly the metabolic system and a subcellular enrichment huge sum of money for passing through itself
Belong to, for example, Trichoderma can by purine system adsorb heavy metal Cu, Trichoderma vacuole also can enriching heavy metal (Fu Kehe etc.,
2014), while the organic acid of Trichoderma generation can also solidify or chelate heavy metal.Moreover, there are also one systems of degradation for Trichoderma
The repair functions such as the organophosphorus pesticide of column and the reduction soil salinization.Research shows that: the processing of Trichoderma atroviride bacterium F6 spore suspension
The leaf mustard seedling crossed significantly improves (LixiangCao etc., 2008) to the adsorption efficiency of Cd and Ni.Therefore microorganism and enrichment are planted
Object, which is used in combination, can produce a variety of rehabilitating soil comprehensive effects.
Patent CN109174937A is proposed with the joint repair system of composite bacteria agent cooperation organic fertilizer and chrysanthemum, repairs weight
The soil of metallic pollution.But the patent handles the feasible method for being enriched the plant of heavy metal there is no proposition, can not will also plant
The plant of plant is as green manure returning to the field to conserve soil.The present invention provides subsequent processing enriching heavy metal plant and as green
The effective ways of fertile returning to the field, not only can be effectively reduced heavy metal content in soil, moreover it is possible to restore soil micro-ecosystem, increasing soil has
Beneficial microorganism is simultaneously increase soil fertility, and is an economical and effective and the plant combined rehabilitating soil of the microorganism-that has a extensive future is new
Technology.
Summary of the invention
It is an object of the invention to overcome above-mentioned the shortcomings of the prior art, a kind of Trichoderma asperellum and pale reddish brown lucerne are provided
The method of Mu renovation of heavy metal polluted soil with combined.This method is by a kind of Trichoderma asperellum GDSF1009 (" Plant Protection
Report ", 2015,42 (6): 1030-1035) deposit number: CGMCC 9512) granule and the special interaction of clover root system, improve
Clover adsorbs the efficiency of heavy metal, while the clover stalk for adsorbing heavy metal is cleaned elution or solidification through organic acid, then pass through
The cellulase that Trichoderma generates is degraded；It is prepared into straw bacterial manure.
The purpose of the present invention is achieved through the following technical solutions:
The present invention relates to a kind of methods of restoration of soil polluted by heavy metal, combine reparation weight with clover using Trichoderma asperellum
Metallic pollution soil.
In the present invention, the Trichoderma asperellum is Trichoderma asperellum GDSF1009；The clover is alfalfa.
As one embodiment of the invention, the application rate 1.0-3.0kg of heavy-metal contaminated soil clover per acre, spine spore
The amount of application of Trichoderma granule is 5-20kg.Under the cooperation dosage, Trichoderma asperellum granule adapts to (purple for promoting
Flower) alfalfa growing, improves the ability of (pale reddish brown) clover absorption heavy metal-polluted soil.
As one embodiment of the invention, the Trichoderma asperellum granule is applied after mixing with organic fertilizer.
The amount of application of the organic fertilizer is 50-150kg per acre.
In the present invention, the Trichoderma asperellum granule is obtained by being prepared via a method which: by trichoderma asperellum bacterium solution
Body fermentation liquid 48-52wt%, corn flour 13.625-17.625wt%, diatomite 14.75-20.75wt%, wheat bran 13.625-
17.625wt%, humic acid 0.4-0.6wt%, citric acid 0.4-0.6wt% are stirred evenly, and extrusion granulation is drying to obtain.
Preferably, the drying is in 47 DEG C of dry 1.5h.
The Trichoderma asperellum liquid fermentation liquid is prepared by the inclusion of following method: fermentation medium is 121
Sterilize 30-60min at DEG C, accesses weight ratio (weight ratio with fermentation medium) 0.5-2.0% Trichoderma asperellum kind daughter bacteria
Kind, control fermentation temperature is 25-35 DEG C, fermentation time 5-10d, until fermentation liquid becomes green.
The every 221.5709kg of fermentation medium contains: corn flour 5-15kg, water 200-220kg, potassium dihydrogen phosphate 760-
772g, magnesium sulfate 80-120g, manganese sulfate 0.4-0.6g, zinc sulfate 0.3-0.5g, sodium nitrate 280-288g, ammonium sulfate 210-
230g, sodium chloride 180-220g.
Preferably, the fermentation medium prepare when, first by inorganic salts potassium dihydrogen phosphate, magnesium sulfate, manganese sulfate, zinc sulfate,
Sodium nitrate, ammonium sulfate, sodium chloride composition dissolve in water, then fermentation medium is made after corn flour and water are sufficiently mixed.
The Trichoderma asperellum seed strain is obtained by being prepared via a method which: Trichoderma asperellum is inoculated in PDA training
Support the trichoderma asperellum strain that base culture is activated；In the trichoderma asperellum strain fermentation of liquid fermentation bacterium culture medium access activation
Obtain the Trichoderma strain.
Preferably, the liquid fermentation Spawn incubation based component:
200 grams of potato
Glucose 20g
Water 1L
Tune pH value is 6-8.
Preferably, the culture specifically: Trichoderma asperellum GDFS1009 is inoculated in PDA culture medium plate, 28 DEG C
In incubator, it is inverted culture 2-3d.
Preferably, the fermentation specifically: in 250 milliliters of triangular flasks, per bottled 100ml liquid fermentation Spawn incubation
Base, sterilize 30min at 121 DEG C, accesses the trichoderma asperellum strain of activation, every bottle bacteria cake 5, shaking speed 180rpm, sends out
28 DEG C of ferment temperature, fermentation time 3d obtains liquid fermentation kind daughter bacteria, i.e., the described Trichoderma asperellum seed strain.
As one embodiment of the invention, method of the invention further includes by the lucerne after restoration of soil polluted by heavy metal
Mu stalk mixes after successively eluting heavy metal processing, neutralisation processing with Trichoderma granule, composting, overlay film, fermentation system
The step of at straw bacterial manure.
Preferably, the clover stalk be harvesting absorption heavy metal alfalfa stem and leaf and and root, the segment being cut into is (preferably
Length is 0.5-2cm).
Preferably, the elution heavy metal processing is using the citric acid solution of 0.1mol/L-0.5mol/L concentration processing institute
State clover stalk 30-50 hours.
Preferably, the neutralisation processing is impregnated 2-3 times for that will elute the clover stalk clear water after heavy metal, each 1-
2 hours.
Preferably, neutralisation treated clover stalk and the mixing ratio of Trichoderma asperellum granule are 80-120:1w/w.
Preferably, it is 4.0U.g that the Trichoderma asperellum granule, which has cellulase activity (CMC) enzymatic activity,-1More than.
Preferably, the composting, overlay film, fermentation specifically: in earth's surface composting 8-12cm stalk layer, overlay film (tool ventilation
Hole), ferment at 25 DEG C -27 DEG C 20-30d.
Compared with prior art, the invention has the following beneficial effects:
1) compared with the repair system of single plant, the ability of plant absorption heavy metal is not only significantly improved, soil is increased
Earth beneficial microbe restores soil micro-ecosystem system；
2) compared with existing microorganism-plant complex repairation system, subsequent processing enriching heavy metal plant is provided simultaneously
As the effective ways of green manure returning to the field, the plant for being enriched heavy metal can not only be effectively treated, moreover it is possible to improve its returning to the field
Soil fertility.
Detailed description of the invention
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention,
Objects and advantages will become more apparent upon:
Fig. 1 is the heavy metal average content schematic diagram in alfalfa and rape body after Pudong experimental field plants 5 months.
Specific embodiment
The following describes the present invention in detail with reference to examples.Following embodiment will be helpful to those skilled in the art
The present invention is further understood, but the invention is not limited in any way.It should be pointed out that those skilled in the art
For, without departing from the inventive concept of the premise, it can also make certain adjustments and improvements.These belong to guarantor of the invention
Protect range.
Embodiment 1
Place: electroplate factory, Kingsoft town Dong Zhangyan mulberry field village is experimental field
The present embodiment is related to the method for a kind of Trichoderma asperellum and clover renovation of heavy metal polluted soil with combined.
A kind of preparation method of Trichoderma granule is provided first, is specifically comprised the following steps:
1, plate strain: Trichoderma asperellum GDFS1009 is inoculated in PDA culture medium plate, in 28 DEG C of incubators；?
Set culture 2-3 days.
PDA culture medium: 200 grams of potatoes are cut into 1cm2Fritter, be boiled with soft fire 30 minutes, 4 layers of filtered through gauze, retain juice；
Be added 20 grams of glucose, 20 grams of agar powders, distilled water is settled to 1 liter, 121 DEG C high pressure steam sterilization 30 minutes.
2, the preparation of liquid spawn: 200 grams of potato, 20 grams of glucose, 1 liter of water are taken, second order fermentation culture solution is configured to
(i.e. liquid fermentation bacterium culture medium), tune pH value are 6-8, are divided in 250 milliliters of triangular flasks 100 milliliters, sterilize at 121 DEG C
30 minutes, and access plate strain (it is cultivated on PDA, bacterium colony edge takes bacteria cake with 7mm punch, and every bottle bacteria cake 5, shaking speed
It is 180 revs/min, 28 DEG C of fermentation temperature, fermentation time is 3 days, the conidium of the cotton-shaped mycelia and dispersion that are largely dissipated
Liquid spawn.
3, prepared by trichoderma liquid fermentation liquid: fermentation medium components are as follows: and 10 kilograms of corn flour, 210 kilograms of water, di(2-ethylhexyl)phosphate
766 grams of hydrogen potassium, 100 grams of magnesium sulfate, 0.5 gram of manganese sulfate, 0.4 gram of zinc sulfate, 284 grams of sodium nitrate, 220 grams of ammonium sulfate, sodium chloride
200 grams, inorganic salts (potassium dihydrogen phosphate, magnesium sulfate, manganese sulfate, zinc sulfate, sodium nitrate, ammonium sulfate, sodium chloride) are first dissolved in into water
In, then solid fermentation culture medium is made after compost (corn flour) and water are sufficiently mixed, solid fermentation culture medium is packed into 300 liters
It in fermentor, sterilizes 30~60 minutes at 121 DEG C, accesses the second class inoculum of weight ratio 0.5%, control fermentation temperature is 28
DEG C, fermentation time 7 days, until compost becomes green i.e. trichoderma liquid fermentation liquid；
4, Trichoderma granule preparation: by mass ratio are as follows: trichoderma liquid fermentation liquid 50wt%, corn flour
15.625wt%, diatomite 17.75wt%, wheat bran 15.625wt%, humic acid 0.5wt%, citric acid 0.5wt%, using stirring
The machine of mixing stirs evenly, and after pelletizer extrusion granulation, the drying 1.5 hours of 47 DEG C of drying machine, finished product is Trichoderma granule.
5, heavy metal pollution of soil back end detects: every tripartite mechanism is detected by national standard method；
6, rehabilitating soil experimental group processing method:
Alfalfa WL525HQ (Beijing right way Eco Science Technology Co., Ltd).1.5kg alfalfa kind is sowed in soil per acre
Organic fertilizer (Guangming Hesitan Livestocks Co., Ltd. Shanghai's offer) 90kg and Trichoderma granule 10kg is administered simultaneously in son.To purple
It is harvested after russian fenugreek herb is mature.
7, rehabilitating soil control group processing method:
Blank control group: 1.5kg alfalfa seed is only sowed per acre
Trichoderma pulvis control group: 1.5kg alfalfa seed is sowed per acre, trichoderma pulvis (Shandong Tylenol medicine is administered simultaneously
Industry Co., Ltd provides) 5kg；
Organic fertilizer control group: 1.5kg alfalfa seed is sowed per acre, organic fertilizer (Shanghai light He Sitan is administered simultaneously
Animal husbandry Co., Ltd provides) 90kg；
8, soil remediation effect detection；
The processing of soil sample: throughout managed with five point sampling plot sampling depth be 15-20cm depth pedotheque, 85
Drying to constant weight at DEG C, grind sieve with 100 mesh sieve pack it is spare.The pedotheque for weighing a certain amount of (0.025g or so), using salt
Acid-nitric acid-perchloric acid wet digestion sample, inductive coupling plasma emission spectrograph (U.S. PerkinElmer PE Optima
7000) content of beary metal in analysis detection soil.Data obtained carry out Mathematical Statistics Analysis with SPSS20.0 software.
Calculation method:
Heavy metal in soil contains after heavy metal in soil content-processing before heavy metal-polluted soil rate of descent (%)=processing
Amount)/handle the preceding content of heavy metal in the soil
Heavy metal-polluted soil declines increase rate (%)=(control group content of beary metal-processing group content of beary metal)/control group
Content of beary metal
Heavy metal-polluted soil averagely declines 40% or more (table 1) after handling soil December.
Table 1, trichoderma and organic fertilizer handle heavy metal-polluted soil rate of descent
Note: it is calculated according to the measured value in soil.
Embodiment 2
Place: the town Pudong Qu Heqing electroplate factory, factory is experimental field
The present embodiment is related to the method for a kind of Trichoderma asperellum and clover renovation of heavy metal polluted soil with combined.
A kind of preparation method of Trichoderma biologic grain agent is provided first, is specifically comprised the following steps:
1, plate strain: Trichoderma asperellum GDFS1009 is inoculated in PDA culture medium plate, in 28 DEG C of incubators,
Set culture 2-3 days；
PDA culture medium: 200 grams of potatoes are cut into the fritter of 1cm2, are boiled with soft fire 30 minutes, 4 layers of filtered through gauze, retain juice；
Be added 20 grams of glucose, 20 grams of agar powders, distilled water is settled to 1 liter, 121 DEG C high pressure steam sterilization 30 minutes；
2, the preparation of liquid spawn: taking 200 grams of potato, 20 grams of glucose, 1 liter of water, be configured to second order fermentation culture solution,
Tune pH value is 6-8, is divided in 250 milliliters of triangular flasks 100 milliliters, sterilizes 30 minutes at 121 DEG C, accesses plate strain, shakes
Bed revolving speed is 180 revs/min, and 28 DEG C of fermentation temperature, fermentation time is 3 days, point of the cotton-shaped mycelia largely dissipated and dispersion
Raw spore liquid spawn；
3, prepared by trichoderma liquid fermentation liquid: fermentation medium components are as follows: and 10 kilograms of maize flour, 210 kilograms of water, di(2-ethylhexyl)phosphate
766 grams of hydrogen potassium, 100 grams of magnesium sulfate, 0.5 gram of manganese sulfate, 0.4 gram of zinc sulfate, 284 grams of sodium nitrate, 220 grams of ammonium sulfate, sodium chloride
200 grams, first inorganic salts are dissolved in water, then solid fermentation culture medium, solid fermentation is made after compost and water are sufficiently mixed
Culture medium is fitted into 300 liters of fermentors, is sterilized 30~60 minutes at 121 DEG C, and the second class inoculum of weight ratio 0.5% is accessed,
Controlling fermentation temperature is 28 DEG C, fermentation time 7 days, until compost becomes the i.e. trichoderma liquid fermentation liquid of green；
4, Trichoderma granule preparation: by mass ratio are as follows: trichoderma liquid fermentation liquid 50wt%, corn flour
15.625wt%, diatomite 68.75wt%, wheat bran 15.625wt%, humic acid 0.5wt%, citric acid 0.5wt%, using stirring
The machine of mixing stirs evenly, and after pelletizer extrusion granulation, the drying 1.5 hours of 47 DEG C of drying machine, finished product is Trichoderma granule.
5, rehabilitating soil experimental group processing method:
Alfalfa WL525HQ (Beijing right way Eco Science Technology Co., Ltd).1.5kg alfalfa kind is sowed in soil per acre
Son, Trichoderma granule 10kg.It is harvested after alfalfa is mature.
6, rehabilitating soil control group processing method:
Rape C K group: rape (Shanghai oil 6).0.6kg rape seed is sowed in soil per acre, is harvested after rape is mature；
Rape+trichoderma granule group: rape (Shanghai oil 6).0.6kg rape seed, Trichoderma particle are sowed in soil per acre
Agent 10kg.It is harvested after rape is mature
Clover CK group: alfalfa WL525HQ (Beijing right way Eco Science Technology Co., Ltd).1.5kg is sowed in soil per acre
Alfalfa seed.It is harvested after alfalfa is mature.(applying the organic fertilizer equally measured in all processing of experimental procedure 5-6)
7, alfalfa is harvested after planting 5 months and rape detects its in-vivo heavy metal content, and processing method is as follows:
The plant sample of the different disposal of acquisition, it is clean with distilled water flushing, its surface moisture is blotted with blotting paper, carefully
It is divided into root, cauline leaf two parts, is put into after finishing in 120 DEG C of baking ovens, is dried for 24 hours in 85 DEG C of constant temperature ovens.Plant after drying
Sample is crushed through pulverizer, is sieved with 100 mesh sieve, and weighing 0.03g or so is packed spare.Disappeared using hydrochloric acid-nitric acid-perchloric acid wet process
Solve sample, inductive coupling plasma emission spectrograph (U.S. PerkinElmer PE Optima 7000) analysis detection plant sample
Content of beary metal in product.Data obtained carry out Mathematical Statistics Analysis with SPSS20.0 software.
See Fig. 1.Both Accumulation of heavy metals amounts of weight Cu, Zn are higher than control group 120.15%, 256.82% in clover body.Oil
Both Accumulation of heavy metals amounts of Cu, Zn are higher than control group 71.51%, 190.43% in dish body.
Embodiment 3
The present embodiment prepares straw bacterial manure after being related to a kind of Trichoderma asperellum and clover renovation of heavy metal polluted soil with combined
Method.Include the following steps:
1, it gathers in clover stalk: adsorbing the alfalfa stem and leaf of heavy metal and (clover stalk is cut into length with pulverizer with root
Degree is 1cm segment)；
2, it elutes heavy metal: clover root, stem section group is handled using various concentrations citric acid solutions such as 0.1mol/L-0.5mol/L
40h is knitted, heavy metal eluting rate reaches 40% or more (table 2).Also, stalk cleaning treatment Chi Chi (side length × depth: 2m × 1m),
It contains into the industrial citric acid 100kg of 0.5mol, can apply repeatedly.
Clover root and stem's heavy metal zinc elute effect after table 2, lemon acid soak
3, clover stalk neutralisation is handled: by the clover stalk of organic acid solution elution heavy metal, impregnating 2-3 with clear water
Secondary, 1-2 hours each, the heavy metal accumulation level in alfalfa tissue is significantly lower than untreated preceding 80% or more, pH6-7.
4, prepare that there is cellulase activity (CMC) and enzymatic activity is 4.0U.g-1The above Trichoderma granule；
5, clover stalk is degraded: the clover stalk section after cleaning mixes (100:1w/w) with Trichoderma granule, in earth's surface
Composting 10cm stalk layer, overlay film (have venthole), and ferment 20-30d at 25 DEG C -27 DEG C, are prepared into straw bacterial manure.
In conclusion the present invention repairs quilt by the heavy metal in Trichoderma asperellum and alfalfa synergistic sorption soil
Contaminated soil.It will be prepared into granule, be applied to pale reddish brown in the Trichoderma asperellum GDSF1009 that alfalfa rhizosphere colonizes
Rhizosphere of alfalfa.Since Trichoderma asperellum granule can stimulate lucerne perennial root system, improves it and adsorb heavy metal ability, therefore the two is formed
The reparation bioplex of efficient absorption heavy metal.Heavy metal adsorption efficiency in plant body reaches 51%-59%, weight in soil
Tenor reduces 58%-60%.Simultaneously by cleaning to rehabilitation plant material, recycling can be recycled.The technology
Heavy metal content in soil not only can be effectively reduced, moreover it is possible to restore soil micro-ecosystem, increase soil beneficial microbe and improve soil
Earth fertility is an economical and effective and the plant combined rehabilitating soil new technology of the microorganism-that has a extensive future.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring substantive content of the invention.
Claims (10)
1. a kind of method of restoration of soil polluted by heavy metal, which is characterized in that combine reparation weight with clover using Trichoderma asperellum
Metallic pollution soil.
2. the method for restoration of soil polluted by heavy metal according to claim 1, which is characterized in that the Trichoderma asperellum is
Trichoderma asperellum GDSF1009；The clover is alfalfa.
3. the method for restoration of soil polluted by heavy metal according to claim 1 or 2, which is characterized in that heavy metal-polluted per acre
The application rate for contaminating soil clover is 1.0-3.0kg, and the amount of application of Trichoderma asperellum granule is 5-20kg.
4. the method for restoration of soil polluted by heavy metal according to claim 3, which is characterized in that the Trichoderma asperellum
Granula is applied after mixing with organic fertilizer.
5. the method for restoration of soil polluted by heavy metal according to claim 4, which is characterized in that the application of the organic fertilizer
Amount is 50-150kg per acre.
6. the method for restoration of soil polluted by heavy metal according to claim 3, which is characterized in that the Trichoderma asperellum
Granula is obtained by being prepared via a method which: by Trichoderma asperellum liquid fermentation liquid 48-52wt%, corn flour 13.625-
17.625wt%, diatomite 14.75-20.75wt%, wheat bran 13.625-17.625wt%, humic acid 0.4-0.6wt%, lemon
Sour 0.4-0.6wt% is stirred evenly, and extrusion granulation is drying to obtain.
7. the method for restoration of soil polluted by heavy metal according to claim 6, which is characterized in that the trichoderma asperellum bacterium solution
Body fermentation liquid is prepared by the inclusion of following method: fermentation medium sterilizes 30-60min at 121 DEG C, accesses weight
Ratio 0.5-2.0% Trichoderma asperellum seed strain, control fermentation temperature is 25-35 DEG C, fermentation time 5-10d, until fermentation
Liquid becomes green.
8. the method for restoration of soil polluted by heavy metal according to claim 7, which is characterized in that the fermentation medium is every
221.5709kg contains: corn flour 5-15kg, water 200-220kg, potassium dihydrogen phosphate 760-772g, magnesium sulfate 80-120g, manganese sulfate
0.4-0.6g, zinc sulfate 0.3-0.5g, sodium nitrate 280-288g, ammonium sulfate 210-230g, sodium chloride 180-220g.
9. the method for restoration of soil polluted by heavy metal according to claim 7, which is characterized in that the trichoderma asperellum strain
Daughter bacteria kind is obtained by being prepared via a method which: Trichoderma asperellum is inoculated in the spine spore wood that PDA culture medium culture is activated
Mould species；The Trichoderma strain is obtained in the trichoderma asperellum strain fermentation of liquid fermentation bacterium culture medium access activation.
10. the method for restoration of soil polluted by heavy metal according to claim 1 or 2, which is characterized in that further include that will repair
Clover stalk after heavy-metal contaminated soil is mixed with Trichoderma granule after successively eluting heavy metal processing, neutralisation processing
It closes, composting, overlay film, the step of straw bacterial manure is made of fermenting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910439868.4A CN110142285B (en) | 2019-05-24 | 2019-05-24 | Method for remedying heavy metal contaminated soil by combination of trichoderma asperellum and alfalfa |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910439868.4A CN110142285B (en) | 2019-05-24 | 2019-05-24 | Method for remedying heavy metal contaminated soil by combination of trichoderma asperellum and alfalfa |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110142285A true CN110142285A (en) | 2019-08-20 |
| CN110142285B CN110142285B (en) | 2021-02-12 |
Family
ID=67592075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910439868.4A Active CN110142285B (en) | 2019-05-24 | 2019-05-24 | Method for remedying heavy metal contaminated soil by combination of trichoderma asperellum and alfalfa |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110142285B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102080052A (en) * | 2010-12-17 | 2011-06-01 | 中国科学院南京土壤研究所 | Trichoderma strain FS10-C for promoting contaminated soil phytoremediation and preparation method of microbial agent of trichoderma strain |
| CN102489499A (en) * | 2011-12-14 | 2012-06-13 | 重庆理工大学 | Method for controlling manganese pollution by cooperation of microorganism and plants |
| CN103074379A (en) * | 2012-11-01 | 2013-05-01 | 南京农业大学 | Biogas fermentation technology for utilization of heavy metal contaminated soil restoration plant material |
| CN104788175A (en) * | 2015-03-30 | 2015-07-22 | 上海交通大学 | Biological trichoderma viride granule as well as preparation method and application thereof |
| CN106434374A (en) * | 2016-09-30 | 2017-02-22 | 湖南省农业生物技术研究中心 | Trichoderma asperellum and application thereof in remediation for heavy metal pollution |
| CN107321780A (en) * | 2017-09-06 | 2017-11-07 | 杭州智仁建筑工程有限公司 | A kind of phytomicroorganism joins the method for restoration of soil polluted by heavy metal |
-
2019
- 2019-05-24 CN CN201910439868.4A patent/CN110142285B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102080052A (en) * | 2010-12-17 | 2011-06-01 | 中国科学院南京土壤研究所 | Trichoderma strain FS10-C for promoting contaminated soil phytoremediation and preparation method of microbial agent of trichoderma strain |
| CN102489499A (en) * | 2011-12-14 | 2012-06-13 | 重庆理工大学 | Method for controlling manganese pollution by cooperation of microorganism and plants |
| CN103074379A (en) * | 2012-11-01 | 2013-05-01 | 南京农业大学 | Biogas fermentation technology for utilization of heavy metal contaminated soil restoration plant material |
| CN104788175A (en) * | 2015-03-30 | 2015-07-22 | 上海交通大学 | Biological trichoderma viride granule as well as preparation method and application thereof |
| CN106434374A (en) * | 2016-09-30 | 2017-02-22 | 湖南省农业生物技术研究中心 | Trichoderma asperellum and application thereof in remediation for heavy metal pollution |
| CN107321780A (en) * | 2017-09-06 | 2017-11-07 | 杭州智仁建筑工程有限公司 | A kind of phytomicroorganism joins the method for restoration of soil polluted by heavy metal |
Non-Patent Citations (2)
| Title |
|---|
| 田晔等: "木霉属真菌在重金属污染土壤生物修复中的应用潜力分析", 《科学技术与工程》 * |
| 钱光人: "《危险废物管理》", 31 July 2004, 化学工业出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110142285B (en) | 2021-02-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105542775B (en) | A kind of passivator and its application for faintly acid cadmium pollution soil | |
| CN106905980B (en) | Heavy metal soil remediation agent, preparation method and application thereof in sludge digestion | |
| CN102513340B (en) | Method for repairing soils polluted by heavy metal Cd | |
| CN106867945A (en) | The microorganism passivator and its preparation, purposes of a kind of repairing heavy metal in soil pollution | |
| CN105734041A (en) | Charcoal loaded PSB passivator preparation and method for restoring Pb polluted soil through charcoal loaded PSB passivator | |
| CN105695354B (en) | The technique and application of superhigh temperature aerobic composting fermentation processing municipal sludge | |
| CN106540959A (en) | A kind of method of plant combined in-situ immobilization heavy-metal composite pollution soil | |
| CN110523774B (en) | Method for removing lead pollution in phosphorite waste land by utilizing indigenous microorganism-plant combination | |
| CN106914484B (en) | Ecological restoration method for rural solid waste polluted soil | |
| CN102093975B (en) | Composite fungicide for rapid degradation of organic waste and applications thereof | |
| CN108689781A (en) | Biomass carbon base soil-repairing agent and preparation method thereof | |
| CN106701097A (en) | Repair agent for treating heavy metal contaminated soil and application thereof | |
| CN104707864B (en) | Compound photosynthetic bacteria preparation for enhancing phytoremediation for heavy metal pollution of soil and preparation method thereof | |
| CN109180316A (en) | A kind of rice Special compound microbial fertilizer and preparation method thereof | |
| CN103071672A (en) | Method for recovering cadmium polluted soil by combining compound microorganism bacterium agent with houttuynia cordata | |
| CN110407649A (en) | A kind of multiple-effect renovation agent of heavy-metal contaminated soil and preparation method thereof | |
| CN107338199A (en) | A kind of general bacterium of pineapple for promoting ground phosphate rock dissolving and its application | |
| CN106734184A (en) | A kind of in-situ remediation method of heavy-metal contaminated soil | |
| CN106244155A (en) | A kind of soil Cr pollution amelioration agent | |
| CN103911331A (en) | Composite microbial agent used for restoring wheat-field heavy metal chromium-contaminated soil | |
| CN108941193A (en) | A kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application | |
| CN107502370A (en) | A kind of preparation method of composite soil modifier | |
| CN109576181A (en) | A kind of modified illite powder microbial bacterial agent and the preparation method and application thereof | |
| CN110038887A (en) | Heavy metal decomposition method in soil | |
| CN110079480A (en) | Colloid series bacillus JD-07, microbial inoculum and the application of glyphosate tolerant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |