CN110129341A - 丙肝病毒基因6a型中国毒株全长感染性克隆及其应用 - Google Patents
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Abstract
丙肝病毒基因组是高度异质性的，根据基因组碱基差异可以将其分为8个基因型和多个基因亚型。来自病人HCV不能直接在体外细胞上培养，通过反向遗传学方法构建流行株的HCV体个感染性克隆是促进HCV基础研究、药物和疫苗研发的重要技术操作平台。基因6型HCV主要流行于我国和东南亚地区，目前还没有基因6型中国毒株的全长感染性克隆。本专利主要内容为建立我国HCV基因6a型的体外细胞感染性克隆CH6acc，并展示其在药物研究上的应用。CH6acc的细胞培养模型可以用于HCV的基础研究、药物和疫苗开发。
Description
技术领域
本专利涉及丙型肝炎病毒细胞培养体系的构建，主要内容为丙肝病毒6a型中国毒株全长感染性克隆的建立与应用。
背景技术
全世界大约有7100万人感染丙型肝炎病毒(HCV)，慢性HCV感染会导致肝硬化甚至肝癌。目前，还没有研发出针对HCV的疫苗。HCV是单股正链RNA病毒，全长大约有9.6kb。HCV基因组由一个大的开放阅读框(ORF)和两端的非编码序列(UTR)组成。ORF被病毒和宿主的蛋白酶切割成10个病毒蛋白，分别为结构蛋白：核心蛋白，包膜蛋白1，包膜蛋白2和非结构蛋白(NS)：P7,NS2,NS3,NS4A,NS4B,NS5A和NS5B。
HCV基因组是多样性的，根据基因组内部的碱基差异可以将其分为个不同的基因型和不少于67个基因亚型。最近有报道发现基因8型病毒。HCV的细胞培养体系的构建能够帮助进行HCV的基础研究以及药物测试和疫苗的研发。基因6型在华南地区比较流行，目前还没有基因6型中国毒株的全长细胞培养体系，即6型的全长感染性克隆。
发明内容
我们通过从HCV感染的病人血清里提取病毒的RNA，反转得到病毒的cDNA并通过PCR的方法得到病毒整个毒株序列，命名为CH6a。CH6a全长克隆不能在Huh7.5系列细胞上复制，将CH6a的NS5B换成JFH1序列之后的嵌合体同样不能在细胞当中培养，但是增加HK6a/JFH1 嵌合体病毒和文献中的7个突变点之后，该嵌合体病毒能在Huh7.5细胞上复制，但是不能得到高峰的病毒。加入GD3a C-NS2的4个适应性突变后在Huh7.5细胞上复制可以达到高峰。
CH6a病毒序列名称如下：
CH6aFL-WT
CH6aC-NS2/JFH1-GFP
CH6aC-NS2_4m/JFH1-GFP
CH6aC-5A_7m/JFH1
CH6aC-5A_11m/JFH1
CH6aC-5A_15m/JFH1
CH6aC-5A_18m/JFH1
CH6aORF_22m
CH6aORF_24m
CH6aORF_25m
CH6aORF_26m
CH6aFL_26m
这个6a型的细胞培养模型可以用于药物和疫苗的研发，以及HCV相关的基础性研究。
附图说明
图1为CH6aC-NS2/JFH1和CH6aC-NS2m4/JFH1转肝癌细胞后的传播图。将CH6a C-NS2重 组体(野生型或具有所示突变)的RNA转录本转染到Huh7.5.1细胞中，分别通过免疫染色或 在荧光显微镜下直接观察HCV核心或NS5A-EGFP抗原。得出HCV阳性细胞的百分比(左y轴 对应线条图)。通过焦点形成单位(FFU)测定感染高峰时(≥80％HCV阳性细胞)上清液中 的HCV感染性滴度[3个平行感染的平均值±标准偏差(SD)，右y轴对应条形图]。(A)野生型CH6a C-NS2重组体的转染。将从第25天和第27天收集的C-NS2病毒的上清液接种到 幼稚细胞以产生传代病毒，并且连续地产生第五代病毒用于序列分析(表1)。(B)CH6a C-NS2_4m的转染，其含有在第五代的C-NS2病毒中鉴定的四个突变(4m，I355M/N416S/I831V /L881F)(表1)。
图2为不同突变组合使CH6a Core-NS5A重组病毒在肝癌细胞里高效复制。(A)具有不 同突变(7m，11m，15m和18m)的CH6a重组体的示意图。7m是来自HK6a 5-5A病毒的F1469L/A1677S(LS)(Li等，2012，PNAS-3月份)，V1555L/I1720F/L1795M(Li等， 2014，Gastroenterology)和来自共有6a亚基因组复制子的K1303R/K1696R的组合(Yu等， 2014，AAC)。从Core-NS2重组体中鉴定出4m(I355M/N416S/I831V/L881F；绿色)(表 1)。从传代的C-5A_11m病毒中鉴定出红色突变(表2)。(B)将具有指定突变的C-5A重组 体的RNA转录本转染到Huh7.5.1-VISI-mCherry细胞中(Zhao等，2017J Virol)，并估计 mCherry阳性细胞的百分比(左侧y轴对应线条)和测定感染峰值时培养物上清液中的HCV 感染性滴度(3次感染的平均值±SD，右侧y轴对应条柱形)。(C)将从第33,35和37天收 集的C-5A_11m病毒连续传代至未感染的新细胞。第1代，第3代，第5代和第8代的病毒 扩散感染和峰值感染性滴度为平行进行的两个实验感染中的一个代表数据。从峰值感染收集 的传代病毒的ORF序列分析见表2。
图3为带不同突变的CH6a ORF和全长感染性克隆在转染细胞里高效复制。A)CH6aORF 和具有22,24,25和26个突变(22m，24m，25m和26m)的全长重组病毒示意图。22m是18m(图2A)加I833T/R932G(来自C-5A_11m病毒)(表2)和D2987G/Y2989F(GF突变)(Li 等，2012，PNAS–3月份)的组合。将从CH6aORF_22m鉴定的突变Q1120K/K1648Q，Q1120K /K1648Q/A2941V和I413T/Q1120K/K1648Q/A2941V(在图B和表3中)克隆到CH6aORF_22m 中分别构建CH6aORF_24m，CH6aORF_25m和CH6aORF_26m。突变由不同颜色表示[黑色，以前 鉴定的突变；绿色，来自C-NS2病毒的4m(详见表1)；红色，来自C-5A_22m病毒(表2)； 蓝色，来自CH6aORF_22m(详见表3)]。病毒对药物的敏感性见图4。(B-D)CH6a ORF和具 有如图所示不同突变的全长重组体的转染。实验估测mCherry阳性细胞的百分比(左侧y轴 对应线条)和测定峰值感染时的HCV感染性滴度(3次感染的平均值±SD，右侧y轴对应条 柱形)。J6/JFH1-NS5AΔ40-EGFP用作阳性对照(Gottwein等，2011,J Virol)。(E)Huh7.5 细胞中CH6aORF_26m和CH6aFL_26m的转染。通过Core免疫染色(左侧y轴对应线)显现HCV 阳性细胞，通过FFU测定(三次感染的平均值±SD，右侧y轴对应条柱形)HCV感染性滴度。
图4为CH6a病毒被simeprevir和sofosbuvir剂量依赖性地抑制。与之前建立的全长感 染性HCV克隆1a(TNcc)，2a(J6cc)和2b(J8cc-HT)比较对药物的敏感性。病毒感染96孔板中的Huh7.5细胞后后24小时加入不同浓度的DAA，并再放置48小时，固定细胞，免疫 染色计数HCV阳性细胞。实验中加入2a嵌合体J65'UTR-NS2/JFH1(Li等，2011PNAS)作 为阳性对照。图中所示值为实验中一式3个重复的平均值±SEM(平均值的标准误差)。
具体实施方式
1.从血清当中提取出病毒CH6a的RNA，反转得到病毒的cDNA。
PCR扩增得到病毒的全部序列,并构建到质粒载体中,全长序列为9641bp(附序列CH6aFL-WT)。
2.构建嵌合体病毒质粒并加入突变(见各附图附表显示的突变)。
3.体外转录的方法从质粒上转录出病毒的RNA，然后转染Huh7.5及系列细胞。
4.检测了病毒滴度及感染获得的适应性突变。
5.检测了病毒对当前临床药物的敏感性。
表1.序列分析CH6a C-NS2重组病毒鉴定出突变。将来自CH6a Core-NS2(C-NS2)转染的Huh7.5.1细胞的培养上清液(第11天和第13天，图1B)接种到细胞中，当病毒扩散感染大多数细胞后收集上清液(定义为第1代病毒)。连续传播病毒并得到第9代病毒。第5代和第9代病毒的C-NS2区域的序列分析鉴定出了突变。表中给出重组病毒的核苷酸和氨基酸位置以及H77参考序列(AF009606)的相应位置。用斜线分隔的两个大写字母表示测序读数中的核苷酸准种(50/50)。点(·)表示与原始核苷酸相同，原始氨基酸以单个字母显示。将四个突变(4m，I355M/N416S/I831V/L881F)克隆到C-NS2中构建C-NS2_4m (图1)。
表2.经历多次传代后的CH6a C-5A_11m病毒的序列分析。将CH6a Core-NS5A(C-5A)转染后的 Huh7.5.1-VISI-mCherry细胞(第33,35和37天；图2B)的培养上清液分别进行两个独立的感染实验(实验1和2)，进行病毒传代，经过连续传代，最后获得第8代病毒。第1代，第3代，第6代和第8代病毒的ORF序列分析发现两个实验的传代病毒中的有许多突变(见感染实验1-表的第I部分；感染实验 2–表的第II部分)。用斜线分隔的一个大写字母和一个小写字母表示测序读数中主要峰/次要峰的核苷酸准种。有关核苷酸注释，请参见表1注解。
a11m是指突变I355M/N416S/I831V/L881F/K1303R/F1469L/V1555L/A1677S/K1696R/I1720F/ L1795M的组合。
第I部分(Part I,CH6a C-5A_11ma,independent infection experiment 1)
第II部分(Part II,CH6a C-5A_11ma,independent infection experiment 2)
表3.第一和第二代CH6a_22m病毒的序列分析结果。将来自CH6aORF_22m(图3A)的培养上清液感染 Huh7.5.1-VISI-mCherry细胞，在新细胞上传代。对第一代和第二代病毒进行ORF测序，发现病毒获得了新的突变。用斜线分隔的两个大写字母表示测序读峰中的核苷酸准种为50/50。点(·)表示与原始核苷酸一样，没有突变。阴影表示该核苷酸被克隆到重组质粒中。CH6aORF_26m(CH6acc)的序列分析没有鉴定出其他突变。将四个突变组合(I413T/Q1120K/K1648Q/A2941V)克隆到Ch6aORF_22m中，构建 CH6aORF_26m(图3)。
序列表
<110> 中山大学
<120> 丙肝病毒基因6a型中国毒株全长感染性克隆及其应用
<141> 2019-01-24
<160> 12
<170> SIPOSequenceListing 1.0
<210> 1
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<213> 丙型肝炎病毒CH6aFL-WT(Hepatitis C virus)
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aaggcgcgcc agccccaggg caggcactgg gctcagcccg gatacccttg gcccctttat 600
ggaaacgagg gctgcgggtg ggcaggttgg ctcctgtccc cccgcggctc ccggccgcac 660
tggggcccca acgacccccg gcgtcgatcc cgcaatctgg gtaaggtcat cgatacccta 720
acgtgtgggt tcgccgatct catggggtac atccccgtcg tgggcgcgcc tttgggcggc 780
gtcgcggctg cgctcgcaca cggtgtgagg gcaatcgagg acgggatcaa ttatgcaaca 840
ggaaatcttc ccggttgctc tttctctatc ttcctcttgg cactactctc gtgcctaaca 900
acgccagctt cggctcttac ctacggcaac tccagtgacc tataccatct cacaaatgat 960
tgccccaact ccagcatcgt gctggaggcg gatgccatga tcttgcattt gcctggatgc 1020
ttgccttgtg taaaggtcga taataagtcc acctgctggc atgctgtgtc ccccacccta 1080
gccataccaa atgcttccac gcccgcaacg gggttccgta ggcacgtgga tcttcttgcg 1140
ggcgctgcgg tggtctgttc atctctgtat atcggggatt tgtgcggttc tctcttccta 1200
gcgggacaac tattcacctt ccagcctcgc cgtcactgga ctgtgcaaga ctgcaactgt 1260
tccatctata caggccacgt cactggccac aggatggctt gggacatgat gatgaactgg 1320
tcacccacaa ccactctggt cctatctagc atcttgaggg tacctgagat ctgtgcgagt 1380
gtgatatttg gtggccactg ggggatacta ctagccgttg cctactttgg tatggctggt 1440
aactggctaa aagtcctggc tgttctgttc ttatttgcag gggtcgaagc aaccaccacc 1500
accggctccg cgataagcaa agctacttcc ggctttaccg cgcttttcaa ccccggtgcc 1560
aggcagaatc tacacctcat caactccaat ggtagctggc atataaacag gactgctttg 1620
aactgcaatg attccctcca tacggggttc atagcgtcac tcttttacgc ccacaagttc 1680
aactcctcgg gctgcccaga gcggatggcc gcatgtaagc cccttgctga cttcaagcag 1740
gggtggggcc aaataaccta caaagtcaac atctcaggcc cctctgacga ccgtccctac 1800
tgttggcatt acgctcccag gccatgtgac gtggtggcag cccgcacggt gtgcggccct 1860
gtttactgct tcacgcctag ccctgttgtg gtaggaacca ctgacaggcg gggcaacccc 1920
acatacacct ggggggagaa tgagacggat gtgttcatgc tggaaagcct tcggccccct 1980
actggggggt ggtttgggtg cacctggatg aacgctacgg gctttaccaa gacctgtggt 2040
gccccgccat gtcagatagt cccaggggat tacaacagct ctgccaatga gcttttgtgc 2100
cccaccgatt gcttccgtaa acatccggga gccacatacc agcggtgtgg atcaggaccc 2160
tggttaacac ctaggtgtct agtggactat ccctacagac tgtggcacta cccctgcact 2220
gtaaacttca ccttgcataa ggtcaggatg tttgtggggg gcgttgagca ccggtttgac 2280
gccgcatgta attggaccag gggcgagcgg tgcgagttac atgacagaga caggattgaa 2340
atgagcccgc tgcttttctc aaccacgcag cttgccatac ttccatgttc attttccacc 2400
atgccggcct tgtcaactgg cctcattcac ctgcatcaga acatagtgga cgtgcagtac 2460
ctctacggag tctcctcgag cgtcacctcg tgggtagtaa agtgggagta cattgtcctg 2520
atgtttctgg ttctggcaga tgctcggatt tgtacgtgtc tctggttaat gctgctcata 2580
agtagggttg aggcagcagt ggagaggctt gtcgtcctca acgcggctag cgccgccggc 2640
actgctggct ggtggtgggc ggtgcttttc ctgtgctgtg cttggtacgt gaaaggtcgc 2700
cttgtgcctg cgtgtaccta catggcgctg ggaatgtggc cattgctctt agcaatcttg 2760
gccctgcctc agcgagcgta tgccatggac aatgagcaag cggcatccct cggagctgtt 2820
ggtctattgg ttatcaccat cttcaccatc acccccatgt acaagaagct attgacctgc 2880
ttcatttggt ggaatcagta tttcctcgcc cgagctgagg ccatggtaca cgagtgggtg 2940
cccgacctac gggttagggg tggtagggat tccatcatct tacttacctg cttgttccat 3000
ccacagctgg ggtttgaggt cactaagatc ctactagcta tcctggcccc tctatacatc 3060
ttgcagtaca gtttgctcaa agtgccttac tttgtgcgcg cccacatact cctgcgtgct 3120
tgcctgcttg ttcgtaggct ggcagggggt aagtacgtgc aggcgtgcct tctgaggttg 3180
ggcgcttgga ctggtaccta tgtttatgac catctcgccc ccctttctgg ctgggctagc 3240
gacggactgc gcgatctagc agttgcagtc gagccggtca ttttctctcc tatggagaag 3300
aaaattatca cctggggtgc ggacactgcc gcgtgtggtg acatcttgag tggtctcccg 3360
gtgtcagcga ggttggggaa tttggtgcta ctaggacccg cggatgatat gcggcgcgga 3420
ggttggaagc ttttggctcc catcactgcg tatgcacagc agacgagggg cctagtcggc 3480
accattgtga ccagcctaac cggacgtgac aaaaatgagg tcgaagggga ggtacaggtg 3540
gtctccacgg ctacccaatc cttcctggcg acctccatta acggtgtcat gtggaccgtt 3600
tatcatgggg ccggttcaaa gactctcgcc ggaccgaaag gaccagtgtg tcaaatgtac 3660
accaacgtgg acaaggacct agtagggtgg ccatctcccc agggagcaag atccctcacc 3720
ccatgtactt gtggctctag tgacctctat ttggtcacga gggaggccga cgtcatcccc 3780
gcaagacgca ggggtgacag ccgtgctgcc ctcctctctc ctaggcccat aagcaccttg 3840
aaaggctctt cgggaggccc cattatgtgt ccctcggggc acgttgtggg actcttccga 3900
gcagccgtat gcacaagggg tgtagcaaaa tccttagatt ttatcccagt ggaaaacatg 3960
gagacgacta tgcgctctcc ctcattcaca gacaattcca caccccctgc ggtgccccag 4020
acctatcagg tggggtacct gcacgcacca acaggcagcg gaaagagcac ccgtgtcccg 4080
gcggcgtacg ctagccaggg ctacaaggtg ttggtcttga acccatcggt ggcggcgacg 4140
cttagctttg gctcgtacat gaggcaagct tacggcgtgg agccgaatgt ccggactggg 4200
gtcaggacta taactacagg gggcgccatc acatactcca catatgggaa attcttggcc 4260
gatgggggat gttccggagg ggcgtatgac atcatcatct gcgatgagtg ccactctaca 4320
gaccctacga cggtgttggg cattggcacg gttctcgacc aggctgagac cgccggggtt 4380
cgtcttaccg tgcttgcaac agcaacgccg ccgggttctg tcaccgtacc ccatcctaac 4440
ataacagaga cagccctccc aactacggga gaaataccat tttatggaaa gggcatccct 4500
cttgagtaca tcaaaggggg aagacacctc atattctgtc actcgaagaa gaagtgcgac 4560
gagctggccg ggaaactgaa gtcactcggc ttaaacgccg tcgcattcta cagaggtgtc 4620
gacgtgtccg ttatccccac ttcgggcgac gtcgtcgtct gcgcgacaga cgcccttatg 4680
accggctaca caggcgattt cgattccgtc atcgactgta acgtagccgt gacacaggtg 4740
gtggatttca gcttggaccc gacattttcc atagagacta ccaccgtccc tcaggatgcg 4800
gtatcacgga gccaacgacg aggccgcacg gggcggggta aaccgggggt gtacaggttt 4860
gtctcccagg gggagaggcc ttcgggtatg ttcgacaccg tcgtcctgtg tgaggcttat 4920
gacacgggat gcgcgtggta cgaactaacc ccttctgaaa caactgtcag gctgagggcc 4980
tatctgaaca ctcctggcct tcccgtatgc caagaccacc tggaattttg ggaaggcgtg 5040
tttactggct tgacccacat agacgcccac tttctgtctc agacgaagca ggggggtgag 5100
aacttcgcgt acctcgtggc ataccaggct acagtatgcg ccagggccaa agcccccccg 5160
ccttcttggg atacgatgtg gaagtgtctc atcagactta aacccaccct taccggcccc 5220
actccacttt tgtatcggct gggggccgta caaaatgagg taataacaac ccaccccata 5280
accaaataca tcatgacctg catgtctgcg gatttggagg tcatcaccag cacgtgggtc 5340
cttgtgggtg gagtcctagc cgcgctcgca gcctactgct tgtcagtggg ttgtgttgtc 5400
atctgtggca ggataacctt gactggcaag cctgctgttg tccctgatcg tgagatcttg 5460
taccagcaat ttgacgagat ggaagagtgc tctaggcaca tcccctacct cgccgagggc 5520
cagcagatcg ccgagcaatt cagacaaaag gtgttgggac tccttcaagc gagcgccaag 5580
caggcagaag aactgaagcc tgctgtccat tccgcgtggc ctagggtgga ggagttttgg 5640
aggaagcaca tgtggaactt tgtcagcggg atccagtatt tggcgggttt atccaccctg 5700
cccggcaacc cagccgtggc atcattgatg tcatttacag cgtcgctgac cagtcctctg 5760
aggacttctc agaccctgct cctcaacata ctcggcggct ggatagccac ccaagtggct 5820
cccccccccg cgtccacagc ttttgtcgtg agcggtctag caggagccac ggttggaagc 5880
atcgggctcg ggagggtgtt ggttgatgtg cttgccggat acggggccgg tgtgtcgggt 5940
gctctagtcg ctttcaagat catgagcggc gagtgcccaa ccaccgaaga catggtcaat 6000
ctgctacccg cgctgttgtc gccaggggct ctcgtggtgg gagtcgtgtg tgctgccatc 6060
ttaagacgcc acgtaggccc tgcggagggt gccaatcagt ggatgaacag gctaatagcc 6120
tttgcatcaa gaggcaacca tgtgtccccg acgcactacg tgcctgagac tgacgcgtca 6180
aaaaatgtga ctcagatact cacttctctt accatcacca gcctactccg caggttacat 6240
cagtgggtca atgaggacac ggccacccct tgcgctacct catggttacg cgacgtgtgg 6300
gactgggtgt gcacagtgct atctgacttt aaagtatggt tgcaagccaa acttttccct 6360
cgcctgccgg ggatcccctt cctctcgtgc caaacgggat ataggggagt ctgggcaggg 6420
gacggggtgt gccacaccac ttgtacctgt ggggccgtga tagctggaca cgtcaaaaat 6480
ggctccatga aaatcacagg acccaagaca tgcagcaaca cttggcacgg gactttccca 6540
atcaacgcca ccactaccgg ccccagcaca ccacgaccag ccctcaacta tcagcgcgct 6600
ctttggcggg tgtccgccga ggactacgtt gaggtgcgga ggttgggcga ctgccactac 6660
gtggtagggg tcactgctga cgggttgaaa tgcccttgcc aggtacctgc acctgaattc 6720
ttcactgagg tcgatggcgt gaggatacac cgttacgcgc caccttgcaa gcccttgctc 6780
agggacgaag tgactttctc tgtgggtctt tcaaactatg tcatagggtc tcagctccct 6840
tgcgagccag agcctgacgt gaccgtggtc acctcaatgc tcacagaccc cgtgcacatc 6900
accgcagaga cggcagcacg gcggttgaag agggggtccc ccccctcttt agccagctca 6960
tcggccagcc agctgtctgc accgtccctc aaggctactt gtacaacctc cagagaccac 7020
ccggacatgg agctcatcga ggccaacctc ctctggaggc aggagatggg aggcaacatc 7080
actcgagtcg agtcagagaa caaagtcgta gtccttgact cctttgagcc tctaaccgct 7140
gagtatgacg agagggaaat ctcagtgtca gctgagtgcc acaagccacc caggcccaaa 7200
ttccctccag ctctcccagt atgggccagg cccgactaca atccacctct cctacaagca 7260
tggcaaatgc ccggatacga gcctccagtc gtgtctggat gtgccgtcgc tccacctaaa 7320
ccggcaccaa ttcctccgcc gaggcggaag aggctagtgc acttggatga gtccacggtc 7380
tcgcacgcct tggcgcagct tgccgacaag gtatttgtgg agagtggtag tgacccggga 7440
cctagttcag actcgggact atcaatagcc agtcctgtcc cgcctgtctc aacaacaccg 7500
gatgacgcct gctcagaagc agagtcctat agctcaatgc cccctcttga gggagagcct 7560
ggtgaccctg acctgagctc aggctcttgg tccactgtga gcgatcagga tgatgtcgtg 7620
tgttgttcca tgtcctattc ctggacgggg gctctaataa caccatgcgc tgcggaggag 7680
gagaagcttc caataaatcc tatgagcaac tcccttataa gacaccataa catggtgtat 7740
tccaccacat cacgcagcgc cagcctccgc cagaagaagg tcacatttga cagactgcaa 7800
gtgtttgacc aacactacca ggatgtacta aaggagatta agcttcgagc gtccacggtg 7860
caggcaaaac tcctatccat agaggaagcc tgtgacctta cgccatcgca ctcagcacgg 7920
tccaagtatg gatatggtgc gcaggacgtt agaagccatg ctagcaaggc cgttgaccac 7980
atccgctccg tgtgggagga cttgctagaa gactctgata ccccaattcc cacgaccatc 8040
atggctaaga atgaagtctt ctgcgtagat ccgtcgaagg gtggacgcaa gccggcacgc 8100
ctaatagttt tcccagactt gagcgtgcgg gtctgcgaga agatggccct atacgacgta 8160
acgcggaagt tgccacaggc cgtgatgggt tcagcatacg gattccagta ctcccctaac 8220
cagagggttg agtacttgct caaaatgtgg cggtcaaaga aagtgcccat gggcttttct 8280
tacgacacca ggtgttttga ttcaaccgtc actgagcggg atatccggac tgagaacgaa 8340
atctatcagt cttgccagct ggatcccgtg gcaaggaggg cagtatcatc cctaacggag 8400
cggctctacg taggcggccc catggtaaac tccaagggac agccatgtgg ctaccgtaga 8460
tgccgcgcca gtggggtgct gcccacgagc atgggaaaca ccctcacatg ctatctgaag 8520
gcacaggccg cctgcagggc ggccaacatc aaagactatg acatgttggt gtgcggagat 8580
gacttagtgg tcatttgtga gagtgctggc gtccaggagg acactgcgtc actgcgagca 8640
ttcacggatg ctatgaccag gtattcagct ccccctggag acgccccgca acctacatat 8700
gaccttgagc tcataacatc atgctcatcc aacgtctccg tcgcccatga cggcaacggg 8760
aagagatatt actacctcac acgtgactgc actactccac ttgcgcgggc tgcatgggag 8820
acagcccgcc acactccagt caattcgtgg ttgggcaaca tcattatgtt tgcccccacg 8880
atatgggtgc gtatggttct gatgacccat tttttctcca tactccagtc gcaagaacaa 8940
ttggagaaag cactcgactt tgacatctac ggagtgacct actccgtctc tccacttgat 9000
ctcccagcaa tcattcaacg actccatggc atggcagcat tttcactcca cggatattct 9060
ccagttgagc tcaatagggt aggggcttgc ctcaggaaac ttggggtacc tcccttgaga 9120
gcctggagac atcgagccag agctgtcaga gccaaactca ttgcccaggg ggggaaagcg 9180
gctatatgcg gtaagtacct ctttaactgg gcggtgaaga ccaaattaaa actcactcca 9240
ctggtctccg cgagcaagct tgacttatca ggctggttcg tggcaggcta cgacgggggg 9300
gacatttatc acagcgtgtc ccaggctcga ccccgtctct tactccttgg cctactccta 9360
ctctcagtag gggtaggcat ctttttgctc cccgctcggt agacgagcgg ccctagcaac 9420
actccataat tccttttccc ttttttattt tttttctttt tttttttttt tttttttttt 9480
tttttttttt tttttttttt tttttaacct ttcttatctg tctatcttct aaacttcttt 9540
cttggtggct ccatcttagc cctagtcacg gctagctgtg aaaggtccgt gagccgcatg 9600
actgcagaga gtgctgatac tggcctctct gcagatcatg t 9641
<210> 2
<211> 10278
<212> DNA
<213> 丙型肝炎病毒CH6aC-NS2/JFH1-GFP(Hepatitis C virus)
<400> 2
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
tgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatttggt ggccactggg ggatactact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcatca actccaatgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgt 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcataag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt atcaccatct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta cttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtaggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgctta tgcccagcaa acacgaggcc tcctgggcgc 3480
catagtggtg agtatgacgg ggcgtgacag gacagaacag gccggggaag tccaaatcct 3540
gtccacagtc tctcagtcct tcctcggaac aaccatctcg ggggttttgt ggactgttta 3600
ccacggagct ggcaacaaga ctctagccgg cttacggggt ccggtcacgc agatgtactc 3660
gagtgctgag ggggacttgg taggctggcc cagcccccct gggaccaagt ctttggagcc 3720
gtgcaagtgt ggagccgtcg acctatatct ggtcacgcgg aacgctgatg tcatcccggc 3780
tcggagacgc ggggacaagc ggggagcatt gctctccccg agacccattt cgaccttgaa 3840
ggggtcctcg ggggggccgg tgctctgccc taggggccac gtcgttgggc tcttccgagc 3900
agctgtgtgc tctcggggcg tggccaaatc catcgatttc atccccgttg agacactcga 3960
cgttgttaca aggtctccca ctttcagtga caacagcacg ccaccggctg tgccccagac 4020
ctatcaggtc gggtacttgc atgctccaac tggcagtgga aagagcacca aggtccctgt 4080
cgcgtatgcc gcccaggggt acaaagtact agtgcttaac ccctcggtag ctgccaccct 4140
ggggtttggg gcgtacctat ccaaggcaca tggcatcaat cccaacatta ggactggagt 4200
caggaccgtg atgaccgggg aggccatcac gtactccaca tatggcaaat ttctcgccga 4260
tgggggctgc gctagcggcg cctatgacat catcatatgc gatgaatgcc acgctgtgga 4320
tgctacctcc attctcggca tcggaacggt ccttgatcaa gcagagacag ccggggtcag 4380
actaactgtg ctggctacgg ccacaccccc cgggtcagtg acaacccccc atcccgatat 4440
agaagaggta ggcctcgggc gggagggtga gatccccttc tatgggaggg cgattcccct 4500
atcctgcatc aagggaggga gacacctgat tttctgccac tcaaagaaaa agtgtgacga 4560
gctcgcggcg gcccttcggg gcatgggctt gaatgccgtg gcatactata gagggttgga 4620
cgtctccata ataccagctc agggagatgt ggtggtcgtc gccaccgacg ccctcatgac 4680
ggggtacact ggagactttg actccgtgat cgactgcaat gtagcggtca cccaagctgt 4740
cgacttcagc ctggacccca ccttcactat aaccacacag actgtcccac aagacgctgt 4800
ctcacgcagt cagcgccgcg ggcgcacagg tagaggaaga cagggcactt ataggtatgt 4860
ttccactggt gaacgagcct caggaatgtt tgacagtgta gtgctttgtg agtgctacga 4920
cgcaggggct gcgtggtacg atctcacacc agcggagacc accgtcaggc ttagagcgta 4980
tttcaacacg cccggcctac ccgtgtgtca agaccatctt gaattttggg aggcagtttt 5040
caccggcctc acacacatag acgcccactt cctctcccaa acaaagcaag cgggggagaa 5100
cttcgcgtac ctagtagcct accaagctac ggtgtgcgcc agagccaagg cccctccccc 5160
gtcctgggac gccatgtgga agtgcctggc ccgactcaag cctacgcttg cgggccccac 5220
acctctcctg taccgtttgg gccctattac caatgaggtc accctcacac accctgggac 5280
gaagtacatc gccacatgca tgcaagctga ccttgaggtc atgaccagca cgtgggtcct 5340
agctggagga gtcctggcag ccgtcgccgc atattgcctg gcgactggat gcgtttccat 5400
catcggccgc ttgcacgtca accagcgagt cgtcgttgcg ccggataagg aggtcctgta 5460
tgaggctttt gatgagatgg aggaatgcgc ctctagggcg gctctcatcg aagaggggca 5520
gcggatagcc gagatgttga agtccaagat ccaaggcttg ctgcagcagg cctctaagca 5580
ggcccaggac atacaacccg ctatgcaggc ttcatggccc aaagtggaac aattttgggc 5640
cagacacatg tggaacttca ttagcggcat ccaatacctc gcaggattgt caacactgcc 5700
agggaacccc gcggtggctt ccatgatggc attcagtgcc gccctcacca gtccgttgtc 5760
gaccagtacc accatccttc tcaacatcat gggaggctgg ttagcgtccc agatcgcacc 5820
acccgcgggg gccaccggct ttgtcgtcag tggcctggtg ggggctgccg tgggcagcat 5880
aggcctgggt aaggtgctgg tggacatcct ggcaggatat ggtgcgggca tttcgggggc 5940
cctcgtcgca ttcaagatca tgtctggcga gaagccctct atggaagatg tcatcaatct 6000
actgcctggg atcctgtctc cgggagccct ggtggtgggg gtcatctgcg cggccattct 6060
gcgccgccac gtgggaccgg gggagggcgc ggtccaatgg atgaacaggc ttattgcctt 6120
tgcttccaga ggaaaccacg tcgcccctac tcactacgtg acggagtcgg atgcgtcgca 6180
gcgtgtgacc caactacttg gctctcttac tataaccagc ctactcagaa gactccacaa 6240
ttggataact gaggactgcc ccatcccatg ctccggatcc tggctccgcg acgtgtggga 6300
ctgggtttgc accatcttga cagacttcaa aaattggctg acctctaaat tgttccccaa 6360
gctgcccggc ctccccttca tctcttgtca aaaggggtac aagggtgtgt gggccggcac 6420
tggcatcatg accacgcgct gcccttgcgg cgccaacatc tctggcaatg tccgcctggg 6480
ctctatgagg atcacagggc ctaaaacctg catgaacacc tggcagggga cctttcctat 6540
caattgctac acggagggcc agtgcgcgcc gaaacccccc acgaactaca agaccgccat 6600
ctggagggtg gcggcctcgg agtacgcgga ggtgacgcag catgggtcgt actcctatgt 6660
aacaggactg accactgaca atctgaaaat tccttgccaa ctaccttctc cagagttttt 6720
ctcctgggtg gacggtgtgc agatccatag gtttgcaccc acaccaaagc cgtttttccg 6780
ggatgaggtc tcgttctgcg ttgggcttaa ttcctatgct gtcgggtccc agcttccctg 6840
tgaacctgag cccgacgcag acgtattgag gtccatgcta acagatccgc cccacatcac 6900
ggcggagact gcggcgcggc gcttggcacg gggatcacct ccatctgagg cgagctcctc 6960
agtgagccag ctatcagcac cgtcgctgcg ggccacctgc accacccaca gcaacaccct 7020
tgagccctca ataccatcgg agtgcatgct ccccaggagc gggtttccac gggccttacc 7080
ggcttgggca cggcctgact acaacccgcc gctcgtggaa tcgtggagga ggccagatta 7140
ccaaccgccc accgttgctg gttgtgctct cccccccccc aagaaggccc cgacgcctcc 7200
cccaaggaga cgccggacag tgggtctgag cgagagcacc atatcagaag ccctccagca 7260
actggccatc aagacctttg gccagccccc ctcgagcggt gatgcaggct cgtccacggg 7320
ggcgggcgcc gccgaatccg gcggtccgac gtcccctggt gagccggccc cctcagagac 7380
aggttccgcc tcctctatgc cccccatggt gagcaagggc gaggagctgt tcaccggggt 7440
ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 7500
cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 7560
caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt 7620
cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 7680
ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 7740
ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 7800
ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 7860
tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 7920
cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 7980
ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 8040
caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 8100
cggcatggac gagctgtaca agctcgaggg ggagcctgga gatccggacc tggagtctga 8160
tcaggtagag cttcaacctc ccccccaggg ggggggggta gctcccggtt cgggctcggg 8220
gtcttggtct acttgctccg aggaggacga taccaccgtg tgctgctcca tgtcatactc 8280
ctggaccggg gctctaataa ctccctgtag ccccgaagag gaaaagttgc caatcaaccc 8340
tttgagtaac tcgctgttgc gataccataa caaggtgtac tgtacaacat caaagagcgc 8400
ctcacagagg gctaaaaagg taacttttga caggacgcaa gtgctcgacg cccattatga 8460
ctcagtctta aaggacatca agctagcggc ttccaaggtc agcgcaaggc tcctcacctt 8520
ggaggaggcg tgccagttga ctccacccca ttctgcaaga tccaagtatg gattcggggc 8580
caaggaggtc cgcagcttgt ccgggagggc cgttaaccac atcaagtccg tgtggaagga 8640
cctcctggaa gacccacaaa caccaattcc cacaaccatc atggccaaaa atgaggtgtt 8700
ctgcgtggac cccgccaagg ggggtaagaa accagctcgc ctcatcgttt accctgacct 8760
cggcgtccgg gtctgcgaga aaatggccct ctatgacatt acacaaaagc ttcctcaggc 8820
ggtaatggga gcttcctatg gcttccagta ctcccctgcc caacgggtgg agtatctctt 8880
gaaagcatgg gcggaaaaga aggaccccat gggtttttcg tatgataccc gatgcttcga 8940
ctcaaccgtc actgagagag acatcaggac cgaggagtcc atataccagg cctgctccct 9000
gcccgaggag gcccgcactg ccatacactc gctgactgag agactttacg taggagggcc 9060
catgttcaac agcaagggtc aaacctgcgg ttacagacgt tgccgcgcca gcggggtgct 9120
aaccactagc atgggtaaca ccatcacatg ctatgtgaaa gccctagcgg cctgcaaggc 9180
tgcggggata gttgcgccca caatgctggt atgcggcgat gacctagtag tcatctcaga 9240
aagccagggg actgaggagg acgagcggaa cctgagagcc ttcacggagg ccatgaccag 9300
gtactctgcc cctcctggtg atccccccag accggaatat gacctggagc taataacatc 9360
ctgttcctca aatgtgtctg tggcgttggg cccgcggggc cgccgcagat actacctgac 9420
cagagaccca accactccac tcgcccgggc tgcctgggaa acagttagac actcccctat 9480
caattcatgg ctgggaaaca tcatccagta tgctccaacc atatgggttc gcatggtcct 9540
aatgacacac ttcttctcca ttctcatggt ccaagacacc ctggaccaga acctcaactt 9600
tgagatgtat ggatcagtat actccgtgaa tcctttggac cttccagcca taattgagag 9660
gttacacggg cttgacgcct tttctatgca cacatactct caccacgaac tgacgcgggt 9720
ggcttcagcc ctcagaaaac ttggggcgcc acccctcagg gtgtggaaga gtcgggctcg 9780
cgcagtcagg gcgtccctca tctcccgtgg agggaaagcg gccgtttgcg gccgatatct 9840
cttcaattgg gcggtgaaga ccaagctcaa actcactcca ttgccggagg cgcgcctact 9900
ggacttatcc agttggttca ccgtcggcgc cggcgggggc gacatttttc acagcgtgtc 9960
gcgcgcccga ccccgctcat tactcttcgg cctactccta cttttcgtag gggtaggcct 10020
cttcctactc cccgctcggt agagcggcac acactaggta cactccatag ctaactgttc 10080
cttttttttt tttttttttt tttttttttt tttttttttt ttttcttttt tttttttttc 10140
cctctttctt cccttctcat cttattctac tttctttctt ggtggctcca tcttagccct 10200
agtcacggct agctgtgaaa ggtccgtgag ccgcatgact gcagagagtg ccgtaactgg 10260
tctctctgca gatcatgt 10278
<210> 3
<211> 10278
<212> DNA
<213> 丙型肝炎病毒CH6aC-NS2_4m/JFH1-GFP(Hepatitis C virus)
<400> 3
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
tgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatttggt ggccactggg ggatgctact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcatca actccagtgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgt 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcataag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt gtcaccatct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta tttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtaggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgctta tgcccagcaa acacgaggcc tcctgggcgc 3480
catagtggtg agtatgacgg ggcgtgacag gacagaacag gccggggaag tccaaatcct 3540
gtccacagtc tctcagtcct tcctcggaac aaccatctcg ggggttttgt ggactgttta 3600
ccacggagct ggcaacaaga ctctagccgg cttacggggt ccggtcacgc agatgtactc 3660
gagtgctgag ggggacttgg taggctggcc cagcccccct gggaccaagt ctttggagcc 3720
gtgcaagtgt ggagccgtcg acctatatct ggtcacgcgg aacgctgatg tcatcccggc 3780
tcggagacgc ggggacaagc ggggagcatt gctctccccg agacccattt cgaccttgaa 3840
ggggtcctcg ggggggccgg tgctctgccc taggggccac gtcgttgggc tcttccgagc 3900
agctgtgtgc tctcggggcg tggccaaatc catcgatttc atccccgttg agacactcga 3960
cgttgttaca aggtctccca ctttcagtga caacagcacg ccaccggctg tgccccagac 4020
ctatcaggtc gggtacttgc atgctccaac tggcagtgga aagagcacca aggtccctgt 4080
cgcgtatgcc gcccaggggt acaaagtact agtgcttaac ccctcggtag ctgccaccct 4140
ggggtttggg gcgtacctat ccaaggcaca tggcatcaat cccaacatta ggactggagt 4200
caggaccgtg atgaccgggg aggccatcac gtactccaca tatggcaaat ttctcgccga 4260
tgggggctgc gctagcggcg cctatgacat catcatatgc gatgaatgcc acgctgtgga 4320
tgctacctcc attctcggca tcggaacggt ccttgatcaa gcagagacag ccggggtcag 4380
actaactgtg ctggctacgg ccacaccccc cgggtcagtg acaacccccc atcccgatat 4440
agaagaggta ggcctcgggc gggagggtga gatccccttc tatgggaggg cgattcccct 4500
atcctgcatc aagggaggga gacacctgat tttctgccac tcaaagaaaa agtgtgacga 4560
gctcgcggcg gcccttcggg gcatgggctt gaatgccgtg gcatactata gagggttgga 4620
cgtctccata ataccagctc agggagatgt ggtggtcgtc gccaccgacg ccctcatgac 4680
ggggtacact ggagactttg actccgtgat cgactgcaat gtagcggtca cccaagctgt 4740
cgacttcagc ctggacccca ccttcactat aaccacacag actgtcccac aagacgctgt 4800
ctcacgcagt cagcgccgcg ggcgcacagg tagaggaaga cagggcactt ataggtatgt 4860
ttccactggt gaacgagcct caggaatgtt tgacagtgta gtgctttgtg agtgctacga 4920
cgcaggggct gcgtggtacg atctcacacc agcggagacc accgtcaggc ttagagcgta 4980
tttcaacacg cccggcctac ccgtgtgtca agaccatctt gaattttggg aggcagtttt 5040
caccggcctc acacacatag acgcccactt cctctcccaa acaaagcaag cgggggagaa 5100
cttcgcgtac ctagtagcct accaagctac ggtgtgcgcc agagccaagg cccctccccc 5160
gtcctgggac gccatgtgga agtgcctggc ccgactcaag cctacgcttg cgggccccac 5220
acctctcctg taccgtttgg gccctattac caatgaggtc accctcacac accctgggac 5280
gaagtacatc gccacatgca tgcaagctga ccttgaggtc atgaccagca cgtgggtcct 5340
agctggagga gtcctggcag ccgtcgccgc atattgcctg gcgactggat gcgtttccat 5400
catcggccgc ttgcacgtca accagcgagt cgtcgttgcg ccggataagg aggtcctgta 5460
tgaggctttt gatgagatgg aggaatgcgc ctctagggcg gctctcatcg aagaggggca 5520
gcggatagcc gagatgttga agtccaagat ccaaggcttg ctgcagcagg cctctaagca 5580
ggcccaggac atacaacccg ctatgcaggc ttcatggccc aaagtggaac aattttgggc 5640
cagacacatg tggaacttca ttagcggcat ccaatacctc gcaggattgt caacactgcc 5700
agggaacccc gcggtggctt ccatgatggc attcagtgcc gccctcacca gtccgttgtc 5760
gaccagtacc accatccttc tcaacatcat gggaggctgg ttagcgtccc agatcgcacc 5820
acccgcgggg gccaccggct ttgtcgtcag tggcctggtg ggggctgccg tgggcagcat 5880
aggcctgggt aaggtgctgg tggacatcct ggcaggatat ggtgcgggca tttcgggggc 5940
cctcgtcgca ttcaagatca tgtctggcga gaagccctct atggaagatg tcatcaatct 6000
actgcctggg atcctgtctc cgggagccct ggtggtgggg gtcatctgcg cggccattct 6060
gcgccgccac gtgggaccgg gggagggcgc ggtccaatgg atgaacaggc ttattgcctt 6120
tgcttccaga ggaaaccacg tcgcccctac tcactacgtg acggagtcgg atgcgtcgca 6180
gcgtgtgacc caactacttg gctctcttac tataaccagc ctactcagaa gactccacaa 6240
ttggataact gaggactgcc ccatcccatg ctccggatcc tggctccgcg acgtgtggga 6300
ctgggtttgc accatcttga cagacttcaa aaattggctg acctctaaat tgttccccaa 6360
gctgcccggc ctccccttca tctcttgtca aaaggggtac aagggtgtgt gggccggcac 6420
tggcatcatg accacgcgct gcccttgcgg cgccaacatc tctggcaatg tccgcctggg 6480
ctctatgagg atcacagggc ctaaaacctg catgaacacc tggcagggga cctttcctat 6540
caattgctac acggagggcc agtgcgcgcc gaaacccccc acgaactaca agaccgccat 6600
ctggagggtg gcggcctcgg agtacgcgga ggtgacgcag catgggtcgt actcctatgt 6660
aacaggactg accactgaca atctgaaaat tccttgccaa ctaccttctc cagagttttt 6720
ctcctgggtg gacggtgtgc agatccatag gtttgcaccc acaccaaagc cgtttttccg 6780
ggatgaggtc tcgttctgcg ttgggcttaa ttcctatgct gtcgggtccc agcttccctg 6840
tgaacctgag cccgacgcag acgtattgag gtccatgcta acagatccgc cccacatcac 6900
ggcggagact gcggcgcggc gcttggcacg gggatcacct ccatctgagg cgagctcctc 6960
agtgagccag ctatcagcac cgtcgctgcg ggccacctgc accacccaca gcaacaccct 7020
tgagccctca ataccatcgg agtgcatgct ccccaggagc gggtttccac gggccttacc 7080
ggcttgggca cggcctgact acaacccgcc gctcgtggaa tcgtggagga ggccagatta 7140
ccaaccgccc accgttgctg gttgtgctct cccccccccc aagaaggccc cgacgcctcc 7200
cccaaggaga cgccggacag tgggtctgag cgagagcacc atatcagaag ccctccagca 7260
actggccatc aagacctttg gccagccccc ctcgagcggt gatgcaggct cgtccacggg 7320
ggcgggcgcc gccgaatccg gcggtccgac gtcccctggt gagccggccc cctcagagac 7380
aggttccgcc tcctctatgc cccccatggt gagcaagggc gaggagctgt tcaccggggt 7440
ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 7500
cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 7560
caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt 7620
cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 7680
ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 7740
ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 7800
ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 7860
tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 7920
cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 7980
ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 8040
caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 8100
cggcatggac gagctgtaca agctcgaggg ggagcctgga gatccggacc tggagtctga 8160
tcaggtagag cttcaacctc ccccccaggg ggggggggta gctcccggtt cgggctcggg 8220
gtcttggtct acttgctccg aggaggacga taccaccgtg tgctgctcca tgtcatactc 8280
ctggaccggg gctctaataa ctccctgtag ccccgaagag gaaaagttgc caatcaaccc 8340
tttgagtaac tcgctgttgc gataccataa caaggtgtac tgtacaacat caaagagcgc 8400
ctcacagagg gctaaaaagg taacttttga caggacgcaa gtgctcgacg cccattatga 8460
ctcagtctta aaggacatca agctagcggc ttccaaggtc agcgcaaggc tcctcacctt 8520
ggaggaggcg tgccagttga ctccacccca ttctgcaaga tccaagtatg gattcggggc 8580
caaggaggtc cgcagcttgt ccgggagggc cgttaaccac atcaagtccg tgtggaagga 8640
cctcctggaa gacccacaaa caccaattcc cacaaccatc atggccaaaa atgaggtgtt 8700
ctgcgtggac cccgccaagg ggggtaagaa accagctcgc ctcatcgttt accctgacct 8760
cggcgtccgg gtctgcgaga aaatggccct ctatgacatt acacaaaagc ttcctcaggc 8820
ggtaatggga gcttcctatg gcttccagta ctcccctgcc caacgggtgg agtatctctt 8880
gaaagcatgg gcggaaaaga aggaccccat gggtttttcg tatgataccc gatgcttcga 8940
ctcaaccgtc actgagagag acatcaggac cgaggagtcc atataccagg cctgctccct 9000
gcccgaggag gcccgcactg ccatacactc gctgactgag agactttacg taggagggcc 9060
catgttcaac agcaagggtc aaacctgcgg ttacagacgt tgccgcgcca gcggggtgct 9120
aaccactagc atgggtaaca ccatcacatg ctatgtgaaa gccctagcgg cctgcaaggc 9180
tgcggggata gttgcgccca caatgctggt atgcggcgat gacctagtag tcatctcaga 9240
aagccagggg actgaggagg acgagcggaa cctgagagcc ttcacggagg ccatgaccag 9300
gtactctgcc cctcctggtg atccccccag accggaatat gacctggagc taataacatc 9360
ctgttcctca aatgtgtctg tggcgttggg cccgcggggc cgccgcagat actacctgac 9420
cagagaccca accactccac tcgcccgggc tgcctgggaa acagttagac actcccctat 9480
caattcatgg ctgggaaaca tcatccagta tgctccaacc atatgggttc gcatggtcct 9540
aatgacacac ttcttctcca ttctcatggt ccaagacacc ctggaccaga acctcaactt 9600
tgagatgtat ggatcagtat actccgtgaa tcctttggac cttccagcca taattgagag 9660
gttacacggg cttgacgcct tttctatgca cacatactct caccacgaac tgacgcgggt 9720
ggcttcagcc ctcagaaaac ttggggcgcc acccctcagg gtgtggaaga gtcgggctcg 9780
cgcagtcagg gcgtccctca tctcccgtgg agggaaagcg gccgtttgcg gccgatatct 9840
cttcaattgg gcggtgaaga ccaagctcaa actcactcca ttgccggagg cgcgcctact 9900
ggacttatcc agttggttca ccgtcggcgc cggcgggggc gacatttttc acagcgtgtc 9960
gcgcgcccga ccccgctcat tactcttcgg cctactccta cttttcgtag gggtaggcct 10020
cttcctactc cccgctcggt agagcggcac acactaggta cactccatag ctaactgttc 10080
cttttttttt tttttttttt tttttttttt tttttttttt ttttcttttt tttttttttc 10140
cctctttctt cccttctcat cttattctac tttctttctt ggtggctcca tcttagccct 10200
agtcacggct agctgtgaaa ggtccgtgag ccgcatgact gcagagagtg ccgtaactgg 10260
tctctctgca gatcatgt 10278
<210> 4
<211> 9636
<212> DNA
<213> 丙型肝炎病毒CH6aC-5A_7m/JFH1(Hepatitis C virus)
<400> 4
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
cgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatttggt ggccactggg ggatactact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcatca actccaatgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgt 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcataag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt atcaccatct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta cttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtaggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgcgta tgcacagcag acgaggggcc tagtcggcac 3480
cattgtgacc agcctaaccg gacgtgacaa aaatgaggtc gaaggggagg tacaggtggt 3540
ctccacggct acccaatcct tcctggcgac ctccattaac ggtgtcatgt ggaccgttta 3600
tcatggggcc ggttcaaaga ctctcgccgg accgaaagga ccagtgtgtc aaatgtacac 3660
caacgtggac aaggacctag tagggtggcc atctccccag ggagcaagat ccctcacccc 3720
atgtacttgt ggctctagtg acctctattt ggtcacgagg gaggccgacg tcatccccgc 3780
aagacgcagg ggtgacagcc gtgctgccct cctctctcct aggcccataa gcaccttgaa 3840
aggctcttcg ggaggcccca ttatgtgtcc ctcggggcac gttgtgggac tcttccgagc 3900
agccgtatgc acaaggggtg tagcaaaatc cttagatttt atcccagtgg aaaacatgga 3960
gacgactatg cgctctccct cattcacaga caattccaca ccccctgcgg tgccccagac 4020
ctatcaggtg gggtacctgc acgcaccaac aggcagcgga aagagcaccc gtgtcccggc 4080
ggcgtacgct agccagggct acaaggtgtt ggtcttgaac ccatcggtgg cggcgacgct 4140
tagctttggc tcgtacatga ggcaagctta cggcgtggag ccgaatgtcc ggactggggt 4200
caggactata actacagggg gcgccatcac atactccaca tatgggagat tcttggccga 4260
tgggggatgt tccggagggg cgtatgacat catcatctgc gatgagtgcc actctacaga 4320
ccctacgacg gtgttgggca ttggcacggt tctcgaccag gctgagaccg ccggggttcg 4380
tcttaccgtg cttgcaacag caacgccgcc gggttctgtc accgtacccc atcctaacat 4440
aacagagaca gccctcccaa ctacgggaga aataccattt tatggaaagg gcatccctct 4500
tgagtacatc aaagggggaa gacacctcat attctgtcac tcgaagaaga agtgcgacga 4560
gctggccggg aaactgaagt cactcggctt aaacgccgtc gcattctaca gaggtgtcga 4620
cgtgtccgtt atccccactt cgggcgacgt cgtcgtctgc gcgacagacg cccttatgac 4680
cggctacaca ggcgatttcg attccgtcat cgactgtaac gtagccgtga cacaggtggt 4740
ggatctcagc ttggacccga cattttccat agagactacc accgtccctc aggatgcggt 4800
atcacggagc caacgacgag gccgcacggg gcggggtaaa ccgggggtgt acaggtttgt 4860
ctcccagggg gagaggcctt cgggtatgtt cgacaccgtc gtcctgtgtg aggcttatga 4920
cacgggatgc gcgtggtacg aactaacccc ttctgaaaca actgtcaggc tgagggccta 4980
tctgaacact cctggccttc ccttatgcca agaccacctg gaattttggg aaggcgtgtt 5040
tactggcttg acccacatag acgcccactt tctgtctcag acgaagcagg ggggtgagaa 5100
cttcgcgtac ctcgtggcat accaggctac agtatgcgcc agggccaaag cccccccgcc 5160
ttcttgggat acgatgtgga agtgtctcat cagacttaaa cccaccctta ccggccccac 5220
tccacttttg tatcggctgg gggccgtaca aaatgaggta ataacaaccc accccataac 5280
caaatacatc atgacctgca tgtctgcgga tttggaggtc atcaccagca cgtgggtcct 5340
tgtgggtgga gtcctagccg cgctcgcatc ctactgcttg tcagtgggtt gtgttgtcat 5400
ctgtggcagg ataaccttga ctggcaggcc tgctgttgtc cctgatcgtg agatcttgta 5460
ccagcaattt gacgagatgg aagagtgctc taggcacttc ccctacctcg ccgagggcca 5520
gcagatcgcc gagcaattca gacaaaaggt gttgggactc cttcaagcga gcgccaagca 5580
ggcagaagaa ctgaagcctg ctgtccattc cgcgtggcct agggtggagg agttttggag 5640
gaagcacatg tggaactttg tcagcgggat ccagtatttg gcgggtttat ccaccctgcc 5700
cggcaaccca gccgtggcat caatgatgtc atttacagcg tcgctgacca gtcctctgag 5760
gacttctcag accctgctcc tcaacatact cggcggctgg atagccaccc aagtggctcc 5820
cccccccgcg tccacagctt ttgtcgtgag cggtctagca ggagccacgg ttggaagcat 5880
cgggctcggg agggtgttgg ttgatgtgct tgccggatac ggggccggtg tgtcgggtgc 5940
tctagtcgct ttcaagatca tgagcggcga gtgcccaacc accgaagaca tggtcaatct 6000
gctacccgcg ctgttgtcgc caggggctct cgtggtggga gtcgtgtgtg ctgccatctt 6060
aagacgccac gtaggccctg cggagggtgc caatcagtgg atgaacaggc taatagcctt 6120
tgcatcaaga ggcaaccatg tgtccccgac gcactacgtg cctgagactg acgcgtcaaa 6180
aaatgtgact cagatactca cttctcttac catcaccagc ctactccgca ggttacatca 6240
gtgggtcaat gaggacacgg ccaccccttg cgctacctca tggttacgcg acgtgtggga 6300
ctgggtgtgc acagtgctat ctgactttaa agtatggttg caagccaaac ttttccctcg 6360
cctgccgggg atccccttcc tctcgtgcca aacgggatat aggggagtct gggcagggga 6420
cggggtgtgc cacaccactt gtacctgtgg ggccgtgata gctggacacg tcaaaaatgg 6480
ctccatgaaa atcacaggac ccaagacatg cagcaacact tggcacggga ctttcccaat 6540
caacgccacc actaccggcc ccagcacacc acgaccagcc ctcaactatc agcgcgctct 6600
ttggcgggtg tccgccgagg actacgttga ggtgcggagg ttgggcgact gccactacgt 6660
ggtaggggtc actgctgacg ggttgaaatg cccttgccag gtacctgcac ctgaattctt 6720
cactgaggtc gatggcgtga ggatacaccg ttacgcgcca ccttgcaagc ccttgctcag 6780
ggacgaagtg actttctctg tgggtctttc aaactatgtc atagggtctc agctcccttg 6840
cgagccagag cctgacgtga ccgtggtcac ctcaatgctc acagaccccg tgcacatcac 6900
cgcagagacg gcagcacggc ggttgaagag ggggtccccc ccctctttag ccagctcatc 6960
ggccagccag ctgtctgcac cgtccctcaa ggctacttgt acaacctcca gagaccaccc 7020
ggacatggag ctcatcgagg ccaacctcct ctggaggcag gagatgggag gcaacatcac 7080
tcgagtcgag tcagagaaca aagtcgtagt ccttgactcc tttgagcctc taaccgctga 7140
gtatgacgag agggaaatct cagtgtcagc tgagtgccac aagccaccca ggcccaaatt 7200
ccctccagct ctcccagtat gggccaggcc cgactacaat ccacctctcc tacaagcatg 7260
gcaaatgccc ggatacgagc ctccagtcgt gtctggatgt gccgtcgctc cacctaaacc 7320
ggcaccaatt cctccgccga ggcggaagag gctagtgcac ttggatgagt ccacggtctc 7380
gcacgccttg gcgcagcttg ccgacaaggt atttgtggag agtggtagtg acccgggacc 7440
tagttcagac tcgggactat caatagccag tcctgtcccg cctgtctcaa caacaccgga 7500
tgacgcctgc tcagaagcag agtcctatag ctcaatgccc cctcttgagg gagagcctgg 7560
tgaccctgac ctgagctcag gctcttggtc cactgtgagc gatcaggatg atgtcgtgtg 7620
ttgttccatg tcatactcct ggaccggggc tctaataact ccctgtagcc ccgaagagga 7680
aaagttgcca atcaaccctt tgagtaactc gctgttgcga taccataaca aggtgtactg 7740
tacaacatca aagagcgcct cacagagggc taaaaaggta acttttgaca ggacgcaagt 7800
gctcgacgcc cattatgact cagtcttaaa ggacatcaag ctagcggctt ccaaggtcag 7860
cgcaaggctc ctcaccttgg aggaggcgtg ccagttgact ccaccccatt ctgcaagatc 7920
caagtatgga ttcggggcca aggaggtccg cagcttgtcc gggagggccg ttaaccacat 7980
caagtccgtg tggaaggacc tcctggaaga cccacaaaca ccaattccca caaccatcat 8040
ggccaaaaat gaggtgttct gcgtggaccc cgccaagggg ggtaagaaac cagctcgcct 8100
catcgtttac cctgacctcg gcgtccgggt ctgcgagaaa atggccctct atgacattac 8160
acaaaagctt cctcaggcgg taatgggagc ttcctatggc ttccagtact cccctgccca 8220
acgggtggag tatctcttga aagcatgggc ggaaaagaag gaccccatgg gtttttcgta 8280
tgatacccga tgcttcgact caaccgtcac tgagagagac atcaggaccg aggagtccat 8340
ataccaggcc tgctccctgc ccgaggaggc ccgcactgcc atacactcgc tgactgagag 8400
actttacgta ggagggccca tgttcaacag caagggtcaa acctgcggtt acagacgttg 8460
ccgcgccagc ggggtgctaa ccactagcat gggtaacacc atcacatgct atgtgaaagc 8520
cctagcggcc tgcaaggctg cggggatagt tgcgcccaca atgctggtat gcggcgatga 8580
cctagtagtc atctcagaaa gccaggggac tgaggaggac gagcggaacc tgagagcctt 8640
cacggaggcc atgaccaggt actctgcccc tcctggtgat ccccccagac cggaatatga 8700
cctggagcta ataacatcct gttcctcaaa tgtgtctgtg gcgttgggcc cgcggggccg 8760
ccgcagatac tacctgacca gagacccaac cactccactc gcccgggctg cctgggaaac 8820
agttagacac tcccctatca attcatggct gggaaacatc atccagtatg ctccaaccat 8880
atgggttcgc atggtcctaa tgacacactt cttctccatt ctcatggtcc aagacaccct 8940
ggaccagaac ctcaactttg agatgtatgg atcagtatac tccgtgaatc ctttggacct 9000
tccagccata attgagaggt tacacgggct tgacgccttt tctatgcaca catactctca 9060
ccacgaactg acgcgggtgg cttcagccct cagaaaactt ggggcgccac ccctcagggt 9120
gtggaagagt cgggctcgcg cagtcagggc gtccctcatc tcccgtggag ggaaagcggc 9180
cgtttgcggc cgatatctct tcaattgggc ggtgaagacc aagctcaaac tcactccatt 9240
gccggaggcg cgcctactgg acttatccag ttggttcacc gtcggcgccg gcgggggcga 9300
catttttcac agcgtgtcgc gcgcccgacc ccgctcatta ctcttcggcc tactcctact 9360
tttcgtaggg gtaggcctct tcctactccc cgctcggtag agcggcacac actaggtaca 9420
ctccatagct aactgttcct tttttttttt tttttttttt tttttttttt tttttttttt 9480
ttcttttttt tttttttccc tctttcttcc cttctcatct tattctactt tctttcttgg 9540
tggctccatc ttagccctag tcacggctag ctgtgaaagg tccgtgagcc gcatgactgc 9600
agagagtgcc gtaactggtc tctctgcaga tcatgt 9636
<210> 5
<211> 9636
<212> DNA
<213> 丙型肝炎病毒CH6aC-5A_11m/JFH1(Hepatitis C virus)
<400> 5
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
cgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatttggt ggccactggg ggatgctact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcatca actccagtgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgt 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcataag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt gtcaccatct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta tttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtaggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgcgta tgcacagcag acgaggggcc tagtcggcac 3480
cattgtgacc agcctaaccg gacgtgacaa aaatgaggtc gaaggggagg tacaggtggt 3540
ctccacggct acccaatcct tcctggcgac ctccattaac ggtgtcatgt ggaccgttta 3600
tcatggggcc ggttcaaaga ctctcgccgg accgaaagga ccagtgtgtc aaatgtacac 3660
caacgtggac aaggacctag tagggtggcc atctccccag ggagcaagat ccctcacccc 3720
atgtacttgt ggctctagtg acctctattt ggtcacgagg gaggccgacg tcatccccgc 3780
aagacgcagg ggtgacagcc gtgctgccct cctctctcct aggcccataa gcaccttgaa 3840
aggctcttcg ggaggcccca ttatgtgtcc ctcggggcac gttgtgggac tcttccgagc 3900
agccgtatgc acaaggggtg tagcaaaatc cttagatttt atcccagtgg aaaacatgga 3960
gacgactatg cgctctccct cattcacaga caattccaca ccccctgcgg tgccccagac 4020
ctatcaggtg gggtacctgc acgcaccaac aggcagcgga aagagcaccc gtgtcccggc 4080
ggcgtacgct agccagggct acaaggtgtt ggtcttgaac ccatcggtgg cggcgacgct 4140
tagctttggc tcgtacatga ggcaagctta cggcgtggag ccgaatgtcc ggactggggt 4200
caggactata actacagggg gcgccatcac atactccaca tatgggagat tcttggccga 4260
tgggggatgt tccggagggg cgtatgacat catcatctgc gatgagtgcc actctacaga 4320
ccctacgacg gtgttgggca ttggcacggt tctcgaccag gctgagaccg ccggggttcg 4380
tcttaccgtg cttgcaacag caacgccgcc gggttctgtc accgtacccc atcctaacat 4440
aacagagaca gccctcccaa ctacgggaga aataccattt tatggaaagg gcatccctct 4500
tgagtacatc aaagggggaa gacacctcat attctgtcac tcgaagaaga agtgcgacga 4560
gctggccggg aaactgaagt cactcggctt aaacgccgtc gcattctaca gaggtgtcga 4620
cgtgtccgtt atccccactt cgggcgacgt cgtcgtctgc gcgacagacg cccttatgac 4680
cggctacaca ggcgatttcg attccgtcat cgactgtaac gtagccgtga cacaggtggt 4740
ggatctcagc ttggacccga cattttccat agagactacc accgtccctc aggatgcggt 4800
atcacggagc caacgacgag gccgcacggg gcggggtaaa ccgggggtgt acaggtttgt 4860
ctcccagggg gagaggcctt cgggtatgtt cgacaccgtc gtcctgtgtg aggcttatga 4920
cacgggatgc gcgtggtacg aactaacccc ttctgaaaca actgtcaggc tgagggccta 4980
tctgaacact cctggccttc ccttatgcca agaccacctg gaattttggg aaggcgtgtt 5040
tactggcttg acccacatag acgcccactt tctgtctcag acgaagcagg ggggtgagaa 5100
cttcgcgtac ctcgtggcat accaggctac agtatgcgcc agggccaaag cccccccgcc 5160
ttcttgggat acgatgtgga agtgtctcat cagacttaaa cccaccctta ccggccccac 5220
tccacttttg tatcggctgg gggccgtaca aaatgaggta ataacaaccc accccataac 5280
caaatacatc atgacctgca tgtctgcgga tttggaggtc atcaccagca cgtgggtcct 5340
tgtgggtgga gtcctagccg cgctcgcatc ctactgcttg tcagtgggtt gtgttgtcat 5400
ctgtggcagg ataaccttga ctggcaggcc tgctgttgtc cctgatcgtg agatcttgta 5460
ccagcaattt gacgagatgg aagagtgctc taggcacttc ccctacctcg ccgagggcca 5520
gcagatcgcc gagcaattca gacaaaaggt gttgggactc cttcaagcga gcgccaagca 5580
ggcagaagaa ctgaagcctg ctgtccattc cgcgtggcct agggtggagg agttttggag 5640
gaagcacatg tggaactttg tcagcgggat ccagtatttg gcgggtttat ccaccctgcc 5700
cggcaaccca gccgtggcat caatgatgtc atttacagcg tcgctgacca gtcctctgag 5760
gacttctcag accctgctcc tcaacatact cggcggctgg atagccaccc aagtggctcc 5820
cccccccgcg tccacagctt ttgtcgtgag cggtctagca ggagccacgg ttggaagcat 5880
cgggctcggg agggtgttgg ttgatgtgct tgccggatac ggggccggtg tgtcgggtgc 5940
tctagtcgct ttcaagatca tgagcggcga gtgcccaacc accgaagaca tggtcaatct 6000
gctacccgcg ctgttgtcgc caggggctct cgtggtggga gtcgtgtgtg ctgccatctt 6060
aagacgccac gtaggccctg cggagggtgc caatcagtgg atgaacaggc taatagcctt 6120
tgcatcaaga ggcaaccatg tgtccccgac gcactacgtg cctgagactg acgcgtcaaa 6180
aaatgtgact cagatactca cttctcttac catcaccagc ctactccgca ggttacatca 6240
gtgggtcaat gaggacacgg ccaccccttg cgctacctca tggttacgcg acgtgtggga 6300
ctgggtgtgc acagtgctat ctgactttaa agtatggttg caagccaaac ttttccctcg 6360
cctgccgggg atccccttcc tctcgtgcca aacgggatat aggggagtct gggcagggga 6420
cggggtgtgc cacaccactt gtacctgtgg ggccgtgata gctggacacg tcaaaaatgg 6480
ctccatgaaa atcacaggac ccaagacatg cagcaacact tggcacggga ctttcccaat 6540
caacgccacc actaccggcc ccagcacacc acgaccagcc ctcaactatc agcgcgctct 6600
ttggcgggtg tccgccgagg actacgttga ggtgcggagg ttgggcgact gccactacgt 6660
ggtaggggtc actgctgacg ggttgaaatg cccttgccag gtacctgcac ctgaattctt 6720
cactgaggtc gatggcgtga ggatacaccg ttacgcgcca ccttgcaagc ccttgctcag 6780
ggacgaagtg actttctctg tgggtctttc aaactatgtc atagggtctc agctcccttg 6840
cgagccagag cctgacgtga ccgtggtcac ctcaatgctc acagaccccg tgcacatcac 6900
cgcagagacg gcagcacggc ggttgaagag ggggtccccc ccctctttag ccagctcatc 6960
ggccagccag ctgtctgcac cgtccctcaa ggctacttgt acaacctcca gagaccaccc 7020
ggacatggag ctcatcgagg ccaacctcct ctggaggcag gagatgggag gcaacatcac 7080
tcgagtcgag tcagagaaca aagtcgtagt ccttgactcc tttgagcctc taaccgctga 7140
gtatgacgag agggaaatct cagtgtcagc tgagtgccac aagccaccca ggcccaaatt 7200
ccctccagct ctcccagtat gggccaggcc cgactacaat ccacctctcc tacaagcatg 7260
gcaaatgccc ggatacgagc ctccagtcgt gtctggatgt gccgtcgctc cacctaaacc 7320
ggcaccaatt cctccgccga ggcggaagag gctagtgcac ttggatgagt ccacggtctc 7380
gcacgccttg gcgcagcttg ccgacaaggt atttgtggag agtggtagtg acccgggacc 7440
tagttcagac tcgggactat caatagccag tcctgtcccg cctgtctcaa caacaccgga 7500
tgacgcctgc tcagaagcag agtcctatag ctcaatgccc cctcttgagg gagagcctgg 7560
tgaccctgac ctgagctcag gctcttggtc cactgtgagc gatcaggatg atgtcgtgtg 7620
ttgttccatg tcatactcct ggaccggggc tctaataact ccctgtagcc ccgaagagga 7680
aaagttgcca atcaaccctt tgagtaactc gctgttgcga taccataaca aggtgtactg 7740
tacaacatca aagagcgcct cacagagggc taaaaaggta acttttgaca ggacgcaagt 7800
gctcgacgcc cattatgact cagtcttaaa ggacatcaag ctagcggctt ccaaggtcag 7860
cgcaaggctc ctcaccttgg aggaggcgtg ccagttgact ccaccccatt ctgcaagatc 7920
caagtatgga ttcggggcca aggaggtccg cagcttgtcc gggagggccg ttaaccacat 7980
caagtccgtg tggaaggacc tcctggaaga cccacaaaca ccaattccca caaccatcat 8040
ggccaaaaat gaggtgttct gcgtggaccc cgccaagggg ggtaagaaac cagctcgcct 8100
catcgtttac cctgacctcg gcgtccgggt ctgcgagaaa atggccctct atgacattac 8160
acaaaagctt cctcaggcgg taatgggagc ttcctatggc ttccagtact cccctgccca 8220
acgggtggag tatctcttga aagcatgggc ggaaaagaag gaccccatgg gtttttcgta 8280
tgatacccga tgcttcgact caaccgtcac tgagagagac atcaggaccg aggagtccat 8340
ataccaggcc tgctccctgc ccgaggaggc ccgcactgcc atacactcgc tgactgagag 8400
actttacgta ggagggccca tgttcaacag caagggtcaa acctgcggtt acagacgttg 8460
ccgcgccagc ggggtgctaa ccactagcat gggtaacacc atcacatgct atgtgaaagc 8520
cctagcggcc tgcaaggctg cggggatagt tgcgcccaca atgctggtat gcggcgatga 8580
cctagtagtc atctcagaaa gccaggggac tgaggaggac gagcggaacc tgagagcctt 8640
cacggaggcc atgaccaggt actctgcccc tcctggtgat ccccccagac cggaatatga 8700
cctggagcta ataacatcct gttcctcaaa tgtgtctgtg gcgttgggcc cgcggggccg 8760
ccgcagatac tacctgacca gagacccaac cactccactc gcccgggctg cctgggaaac 8820
agttagacac tcccctatca attcatggct gggaaacatc atccagtatg ctccaaccat 8880
atgggttcgc atggtcctaa tgacacactt cttctccatt ctcatggtcc aagacaccct 8940
ggaccagaac ctcaactttg agatgtatgg atcagtatac tccgtgaatc ctttggacct 9000
tccagccata attgagaggt tacacgggct tgacgccttt tctatgcaca catactctca 9060
ccacgaactg acgcgggtgg cttcagccct cagaaaactt ggggcgccac ccctcagggt 9120
gtggaagagt cgggctcgcg cagtcagggc gtccctcatc tcccgtggag ggaaagcggc 9180
cgtttgcggc cgatatctct tcaattgggc ggtgaagacc aagctcaaac tcactccatt 9240
gccggaggcg cgcctactgg acttatccag ttggttcacc gtcggcgccg gcgggggcga 9300
catttttcac agcgtgtcgc gcgcccgacc ccgctcatta ctcttcggcc tactcctact 9360
tttcgtaggg gtaggcctct tcctactccc cgctcggtag agcggcacac actaggtaca 9420
ctccatagct aactgttcct tttttttttt tttttttttt tttttttttt tttttttttt 9480
ttcttttttt tttttttccc tctttcttcc cttctcatct tattctactt tctttcttgg 9540
tggctccatc ttagccctag tcacggctag ctgtgaaagg tccgtgagcc gcatgactgc 9600
agagagtgcc gtaactggtc tctctgcaga tcatgt 9636
<210> 6
<211> 9636
<212> DNA
<213> 丙型肝炎病毒CH6aC-5A_15m/JFH1(Hepatitis C virus)
<400> 6
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
cgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatttggt ggccactggg ggatgctact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcatca actccagtgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgc 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcatgag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt gtcaccatct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta tttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtaggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgcgta tgcacagcag acgaggggcc tagtcggcac 3480
cattgtgacc agcctaaccg gacgtgacaa aaatgaggtc gaaggggagg tacaggtggt 3540
ctccacggct acccaatcct tcctggcgac ctccattaac ggtgtcatgt ggaccgttta 3600
tcatggggcc ggttcaaaga ctctcgccgg accgaaagga ccagtgtgtc aaatgtacac 3660
caacgtggac aaggacctag tagggtggcc atctccccag ggagcaagat ccctcacccc 3720
atgtacttgt ggctctagtg acctctattt ggtcacgagg gaggccgacg tcatccccgc 3780
aagacgcagg ggtgacagcc gtgctgccct cctctctcct aggcccataa gcaccttgaa 3840
aggctcttcg ggaggcccca ttatgtgtcc ctcggggcac gttgtgggac tcttccgagc 3900
agccgtatgc acaaggggtg tagcaaaatc cttagatttt atcccagtgg aaaacatgga 3960
gacgactatg cgctctccct cattcacaga caattccaca ccccctgcgg tgccccagac 4020
ctatcaggtg gggtacctgc acgcaccaac aggcagcgga aagagcaccc gtgtcccggc 4080
ggcgtacgct agccagggct acaaggtgtt ggtcttgaac ccatcggtgg cggcgacgct 4140
tagctttggc tcgtacatga ggcaagctta cggcgtggag ccgaatgtcc gggctggggt 4200
caggactata actacagggg gcgccatcac atactccaca tatgggagat tcttggccga 4260
tgggggatgt tccggagggg cgtatgacat catcatctgc gatgagtgcc actctacaga 4320
ccctacgacg gtgttgggca ttggcacggt tctcgaccag gctgagaccg ccggggttcg 4380
tcttaccgtg cttgcaacag caacgccgcc gggttctgtc accgtacccc atcctaacat 4440
aacagagaca gccctcccaa ctacgggaga aataccattt tatggaaagg gcatccctct 4500
tgagtacatc aaagggggaa gacacctcat attctgtcac tcgaagaaga agtgcgacga 4560
gctggccggg aaactgaagt cactcggctt aaacgccgtc gcattctaca gaggtgtcga 4620
cgtgtccgtt atccccactt cgggcgacgt cgtcgtctgc gcgacagacg cccttatgac 4680
cggctacaca ggcgatttcg attccgtcat cgactgtaac gtagccgtga cacaggtggt 4740
ggatctcagc ttggacccga cattttccat agagactacc accgtccctc aggatgcggt 4800
atcacggagc caacgacgag gccgcacggg gcggggtaaa ccgggggtgt acaggtttgt 4860
ctcccagggg gagaggcctt cgggtatgtt cgacaccgtc gtcctgtgtg aggcttatga 4920
cacgggatgc gcgtggtacg aactaacccc ttctgaaaca actgtcaggc tgagggccta 4980
tctgaacact cctggccttc ccttatgcca agaccacctg gaattttggg aaggcgtgtt 5040
tactggcttg acccacatag acgcccactt tctgtctcag acgaagcagg ggggtgagaa 5100
cttcgcgtac ctcgtggcat accaggctac agtatgcgcc agggccaaag cccccccgcc 5160
ttcttgggat acgatgtgga agtgtctcat cagacttaaa cccaccctta ccggccccac 5220
tccacttttg tatcggctgg gggccgtaca aaatgaggta ataacaaccc accccataac 5280
caaatacatc atgacctgca tgtctgcgga tttggaggtc atcaccagca cgtgggtcct 5340
tgtgggtgga gtcctagccg cgctcgcatc ctactgcttg tcagtgggtt gtgttgtcat 5400
ctgtggcagg ataaccttga ctggcaggcc tgctgttgtc cctgatcgtg agatcttgta 5460
ccagcaattt gacgagatgg aagagtgctc taggcacttc ccctacctcg ccgagggcca 5520
gcagatcgcc gagcaattca gacaaaaggt gttgggactc cttcaagcga gcgccaagca 5580
ggcagaagaa ctgaagcctg ctgtccattc cgcgtggcct agggtggagg agttttggag 5640
gaagcacatg tggaactttg tcagcgggat ccagtatttg gcgggtttat ccaccctgcc 5700
cggcaaccca gccgtggcat caatgatgtc atttacagcg tcgctgacca gtcctctgag 5760
gacttctcag accctgctcc tcaacatact cggcggctgg atagccaccc aagtggctcc 5820
cccccccgcg tccacagctt ttgtcgtgag cggtctagca ggagccacgg ttggaagcat 5880
cgggctcggg agggtgttgg ttgatgtgct tgccggatac ggggccggtg tgtcgggtgc 5940
tctagtcgct ttcaagatca tgagcggcga gtgcccaacc accgaagaca tggtcaatct 6000
gctacccgcg ctgttgtcgc caggggctct cgtggtggga gtcgtgtgtg ctgccatctt 6060
aagacgccac gtaggccctg cggagggtgc caatcagtgg atgaacaggc taatagcctt 6120
tgcatcaaga ggcaaccatg tgtccccgac gcactacgtg cctgagactg acgcgtcaaa 6180
aaatgtgact cagatactca cttctcttac catcaccagc ctactccgca ggttacatca 6240
gtgggtcaat gaggacacgg ccaccccttg cgctacctca tggttacgcg acgtgtggga 6300
ctgggtgtgc acagtgctat ctgactttaa agtatggttg caagccaaac ttttccctcg 6360
cctgccgggg atccccttcc tctcgtgcca aacgggatat aggggagtct gggcagggga 6420
cggggtgtgc cacaccactt gtacctgtgg ggccgtgata gctggacacg tcaaaaatgg 6480
ctccatgaaa atcacaggac ccaagacatg cagcaacact tggcacggga ctttcccaat 6540
caacgccacc actaccggcc ccagcacacc acgaccagcc ctcaactatc agcgcgctct 6600
ttggcgggtg tccgccgagg actacgttga ggtgcggagg ttgggcgact gccactacgt 6660
ggtaggggtc actgctgacg ggttgaaatg cccttgccag gtacctgcac ctgaattctt 6720
cactgaggtc gatggcgtga ggatacaccg ttacgcgcca ccttgcaagc ccttgctcag 6780
ggacgaagtg actttctctg tgggtctttc aaactatgtc atagggtctc agctcccttg 6840
cgagccagag cctgacgtga ccgtggtcac ctcaatgctc acagaccccg tgcacatcac 6900
cgcagagacg gcagcacggc ggttgaagag ggggtccccc ccctctttag ccagctcatc 6960
ggccagccag ctgtctgcac cgtccctcaa ggctacttgt acaacctcca gagaccaccc 7020
ggacatggag ctcatcgagg ccaacctcct ctggaggcag gagatgggag gcaacatcac 7080
tcgagtcgag tcagagaaca aagtcgtagt ccttgactcc tttgagcctc taaccgctga 7140
gtatgacgag agggaaatct cagtgtcagc tgagtgccac aagccaccca ggcccaaatt 7200
ccctccagct ctcccagtat gggccaggcc cgactacaat ccacctctcc tacaagcatg 7260
gcaaatgccc ggatacgagc ctccagtcgt gtctggatgt gccgtcgctc cacctaaacc 7320
ggcaccaatt cctccgccga ggcggaagag gctagtgcac ttggatgagt ccacggtctc 7380
gcacgccttg gcgcagcttg ccgacaaggt atttgtggag agtggtagtg acccgggacc 7440
tagttcagac tcgggactat caatagccag tcctgtcccg cctgtctcaa caacaccgga 7500
tgacgcctgc tcagaagcag agtcctatag ctcaatgccc cctcttgagg gagagcctgg 7560
tgaccctgac ctgagctcag gctcttggtc cactgtgagc gatcaggatg atgtcgtgtg 7620
ttgttccatg tcatactcct ggaccggggc tctaataact ccctgtagcc ccgaagagga 7680
aaagttgcca atcaaccctt tgagtaactc gctgttgcga taccataaca aggtgtactg 7740
tacaacatca aagagcgcct cacagagggc taaaaaggta acttttgaca ggacgcaagt 7800
gctcgacgcc cattatgact cagtcttaaa ggacatcaag ctagcggctt ccaaggtcag 7860
cgcaaggctc ctcaccttgg aggaggcgtg ccagttgact ccaccccatt ctgcaagatc 7920
caagtatgga ttcggggcca aggaggtccg cagcttgtcc gggagggccg ttaaccacat 7980
caagtccgtg tggaaggacc tcctggaaga cccacaaaca ccaattccca caaccatcat 8040
ggccaaaaat gaggtgttct gcgtggaccc cgccaagggg ggtaagaaac cagctcgcct 8100
catcgtttac cctgacctcg gcgtccgggt ctgcgagaaa atggccctct atgacattac 8160
acaaaagctt cctcaggcgg taatgggagc ttcctatggc ttccagtact cccctgccca 8220
acgggtggag tatctcttga aagcatgggc ggaaaagaag gaccccatgg gtttttcgta 8280
tgatacccga tgcttcgact caaccgtcac tgagagagac atcaggaccg aggagtccat 8340
ataccaggcc tgctccctgc ccgaggaggc ccgcactgcc atacactcgc tgactgagag 8400
actttacgta ggagggccca tgttcaacag caagggtcaa acctgcggtt acagacgttg 8460
ccgcgccagc ggggtgctaa ccactagcat gggtaacacc atcacatgct atgtgaaagc 8520
cctagcggcc tgcaaggctg cggggatagt tgcgcccaca atgctggtat gcggcgatga 8580
cctagtagtc atctcagaaa gccaggggac tgaggaggac gagcggaacc tgagagcctt 8640
cacggaggcc atgaccaggt actctgcccc tcctggtgat ccccccagac cggaatatga 8700
cctggagcta ataacatcct gttcctcaaa tgtgtctgtg gcgttgggcc cgcggggccg 8760
ccgcagatac tacctgacca gagacccaac cactccactc gcccgggctg cctgggaaac 8820
agttagacac tcccctatca attcatggct gggaaacatc atccagtatg ctccaaccat 8880
atgggttcgc atggtcctaa tgacacactt cttctccatt ctcatggtcc aagacaccct 8940
ggaccagaac ctcaactttg agatgtatgg atcagtatac tccgtgaatc ctttggacct 9000
tccagccata attgagaggt tacacgggct tgacgccttt tctatgcaca catactctca 9060
ccacgaactg acgcgggtgg cttcagccct cagaaaactt ggggcgccac ccctcagggt 9120
gtggaagagt cgggctcgcg cagtcagggc gtccctcatc tcccgtggag ggaaagcggc 9180
cgtttgcggc cgatatctct tcaattgggc ggtgaagacc aagctcaaac tcactccatt 9240
gccggaggcg cgcctactgg acttatccag ttggttcacc gtcggcgccg gcgggggcga 9300
catttttcac agcgtgtcgc gcgcccgacc ccgctcatta ctcttcggcc tactcctact 9360
tttcgtaggg gtaggcctct tcctactccc cgctcggtag agcggcacac actaggtaca 9420
ctccatagct aactgttcct tttttttttt tttttttttt tttttttttt tttttttttt 9480
ttcttttttt tttttttccc tctttcttcc cttctcatct tattctactt tctttcttgg 9540
tggctccatc ttagccctag tcacggctag ctgtgaaagg tccgtgagcc gcatgactgc 9600
agagagtgcc gtaactggtc tctctgcaga tcatgt 9636
<210> 7
<211> 9636
<212> DNA
<213> 丙型肝炎病毒CH6aC-5A_18m/JFH1(Hepatitis C virus)
<400> 7
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
cgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatttggt ggccactggg ggatgctact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcatca actccagtgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgt 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcataag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt gtcaccatct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta tttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtaggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgcgta tgcacagcag acgaggggcc tagtcggcac 3480
cattgtgacc agcctaaccg gacgtgacaa aaatgaggtc gaaggggagg tacaggtggt 3540
ctccacggct acccaatcct tcctggcgac ctccattaac ggtgtcatgt ggaccgttta 3600
tcatggggcc ggttcaaaga ctctcgccgg accgaaagga ccagtgtgtc aaatgtacac 3660
caacgtggac aaggacctag tagggtggcc atctccccag ggagcaagat ccctcacccc 3720
atgtacttgt ggctctagtg acctctattt ggtcacgagg gaggccgacg tcatccccgc 3780
aagacgcagg ggtgacagcc gtgctgccct cctctctcct aggcccataa gcaccttgaa 3840
aggctcttcg ggaggcccca ttatgtgtcc ctcggggcac gttgtgggac tcttccgagc 3900
agccgtatgc acaaggggtg tagcaaaatc cttagatttt atcccagtgg aaaacatgga 3960
gacgactatg cgctctccct cattcacaga caattccaca ccccctgcgg tgccccagac 4020
ctatcaggtg gggtacctgc acgcaccaac aggcagcgga aagagcaccc gtgtcccggc 4080
ggcgtacgct agccagggct acaaggtgtt ggtcttgaac ccatcggtgg cggcgacgct 4140
tagctttggc tcgtacatga ggcaagctta cggcgtggag ccgaatgtcc gggctggggt 4200
caggactata actacagggg gcgccatcac atactccaca tatgggagat tcttggccga 4260
tgggggatgt tccggagggg cgtatgacat catcatctgc gatgagtgcc actctacaga 4320
ccctacgacg gtgttgggca ttggcacggt tctcgaccag gctgagaccg ccggggttcg 4380
tcttaccgtg cttgcaacag caacgccgcc gggttctgtc accgtacccc atcctaacat 4440
aacagagata gccctcccaa ctacgggaga aataccattt tatggaaagg gcatccctct 4500
tgagtacatc aaagggggaa gccacctcat attctgtcac tcgaagaaga agtgcgacga 4560
gctggccggg aaactgaagt cactcggctt aaacgccgtc gcattctaca gaggtgtcga 4620
cgtgtccgtt atccccactt cgggcgacgt cgtcgtctgc gcgacagacg cccttatgac 4680
cggctacaca ggcgatttcg attccgtcat cgactgtaac gtagccgtga cacaggtggt 4740
ggatctcagc ttggacccga cattttccat agagactacc accgtccctc aggatgcggt 4800
atcacggagc caacgacgag gccgcacggg gcggggtaaa ccgggggtgt acaggtttgt 4860
ctcccagggg gagaggcctt cgggtatgtt cgacaccgtc gtcctgtgtg aggcttatga 4920
cacgggatgc gcgtggtacg aactaacccc ttctgaaaca actgtcaggc tgagggccta 4980
tctgaacact cctggccttc ccttatgcca agaccacctg gaattttggg aaggcgtgtt 5040
tactggcttg acccacatag acgcccactt tctgtctcag acgaagcagg ggggtgagaa 5100
cttcgcgtac ctcgtggcat accaggctac agtatgcgcc agggccaaag cccccccgcc 5160
ttcttgggat acgatgtgga agtgtctcat cagacttaaa cccaccctta ccggccccac 5220
tccacttttg tatcggctgg gggccgtaca aaatgaggta ataacaaccc accccataac 5280
caaatacatc atgacctgca tgtctgcgga tttggaggtc atcaccagca cgtgggtcct 5340
tgtgggtgga gtcctagccg cgctcgcatc ctactgcttg tcagtgggtt gtgttgtcat 5400
ctgtggcagg ataaccttga ctggcaggcc tgctgttgtc cctgatcgtg agatcttgta 5460
ccagcaattt gacgagatgg aagagtgctc taggcacttc ccctacctcg ccgagggcca 5520
gcagatcgcc gagcaattca gacaaaaggt gttgggactc cttcaagcga gcgccaagca 5580
ggcagaagaa ctgaagcctg ctgtccattc cgcgtggcct agggtggagg agttttggag 5640
gaagcacatg tggaactttg tcagcgggat ccagtatttg gcgggtttat ccaccctgcc 5700
cggcaaccca gccgtggcat caatgatgtc atttacagcg tcgctgacca gtcctctgag 5760
gacttctcag accctgctcc tcaacatact cggcggctgg atagccaccc aagtggctcc 5820
cccccccgcg tccacagctt ttgtcgtgag cggtctagca ggagccacgg ttggaagcat 5880
cgggctcggg agggtgttgg ttgatgtgct tgccggatac ggggccggtg tgtcgggtgc 5940
tctagtcgct ttcaagatca tgagcggcga gtgcccaacc accgaagaca tggtcaatct 6000
gctacccgcg ctgttgtcgc caggggctct cgtggtggga gtcgtgtgtg ctgccatctt 6060
aagacgccac gtaggccctg cggagggtgc caatcagtgg atgaacaggc taatagcctt 6120
tgcatcaaga ggcaaccatg tgtccccgac gcactacgtg cctgagactg acgcgtcaaa 6180
aaatgtgact cagatactca cttctcttac catcaccagc ctactccgca ggttacatca 6240
gtgggtcaat gaggacacgg ccaccccttg cgctacctca tggttacgcg acgtgtggga 6300
ctgggtgtgc acagtgctat ctgactttaa agtatggttg caagccaaac ttttccctcg 6360
cctgccgggg atccccttcc tctcgtgcca aacgggatat aggggagtct gggcagggga 6420
cggggtgtgc cacaccactt gtacctgtgg ggccgtgata gctggacacg tcaaaaatgg 6480
ctccatgaaa atcacaggac ccaagacatg cagcaacact tggcacggga ctttcccaat 6540
caacgccacc actaccggcc ccagcacacc acgaccagcc ctcaactatc agcgcgctct 6600
ttggcgggtg tccgccgagg actacgttga ggtgcggagg ttgggcgact gccactacgt 6660
ggtaggggtc actgctgacg ggttgaaatg cccttgccag gtacctgcac ctgaattctt 6720
cactgaggtc gatggcgtga ggatacaccg ttacgcgcca ccttgcaagc ccttgctcag 6780
ggacgaagtg actttctctg tgggtctttc aaactatgtc atagggtctc agctcccttg 6840
cgagccagag cctgacgtga ccgtggtcac ctcaatgctc acagaccccg tgcacatcac 6900
cgcagagacg gcagcacggc ggttgaagag ggggtccccc ccctctttag ccagctcatc 6960
ggccagccag ctgtctgcac cgtccctcaa ggctacttgc acaacctcca gagaccaccc 7020
ggacatggag ctcatcgagg ccaacctcct ctggaggcag gagatgggag gcaacatcac 7080
tcgagtcgag tcagagaaca aagtcgtagt ccttgactcc tttgagcctc taaccgctga 7140
gtatgacgag agggaaatct cagtgtcagc tgagtgccac aagccaccca ggcccaaatt 7200
ccctccagct cttccagtat gggccaggcc cgactacaat ccacctctcc tacaagcatg 7260
gcaaatgccc ggatacgagc ctccagtcgt gtctggatgt gccgtcgctc cacctaaacc 7320
ggcaccaatt cctccgccga ggcggaagag gctagtgcac ttggatgagt ccacggtctc 7380
gcacgccttg gcgcagcttg ccgacaaggt atgtgtggag agtggtagtg acccgggacc 7440
tagttcagac tcgggactat caatagccag tcctgtcccg cctgtctcaa caacaccgga 7500
tgccgcctgc tcaggagcag agtcctatag ctcaatgccc cctcttgagg gagagcctgg 7560
tgaccctgac ctgagctcag gctcttggtc cactgtgagc gatcagggtg atgtcgtgtg 7620
ttgttccatg tcatactcct ggaccggggc tctaataact ccctgtagcc ccgaagagga 7680
aaagttgcca atcaaccctt tgagtaactc gctgttgcga taccataaca aggtgtactg 7740
tacaacatca aagagcgcct cacagagggc taaaaaggta acttttgaca ggacgcaagt 7800
gctcgacgcc cattatgact cagtcttaaa ggacatcaag ctagcggctt ccaaggtcag 7860
cgcaaggctc ctcaccttgg aggaggcgtg ccagttgact ccaccccatt ctgcaagatc 7920
caagtatgga ttcggggcca aggaggtccg cagcttgtcc gggagggccg ttaaccacat 7980
caagtccgtg tggaaggacc tcctggaaga cccacaaaca ccaattccca caaccatcat 8040
ggccaaaaat gaggtgttct gcgtggaccc cgccaagggg ggtaagaaac cagctcgcct 8100
catcgtttac cctgacctcg gcgtccgggt ctgcgagaaa atggccctct atgacattac 8160
acaaaagctt cctcaggcgg taatgggagc ttcctatggc ttccagtact cccctgccca 8220
acgggtggag tatctcttga aagcatgggc ggaaaagaag gaccccatgg gtttttcgta 8280
tgatacccga tgcttcgact caaccgtcac tgagagagac atcaggaccg aggagtccat 8340
ataccaggcc tgctccctgc ccgaggaggc ccgcactgcc atacactcgc tgactgagag 8400
actttacgta ggagggccca tgttcaacag caagggtcaa acctgcggtt acagacgttg 8460
ccgcgccagc ggggtgctaa ccactagcat gggtaacacc atcacatgct atgtgaaagc 8520
cctagcggcc tgcaaggctg cggggatagt tgcgcccaca atgctggtat gcggcgatga 8580
cctagtagtc atctcagaaa gccaggggac tgaggaggac gagcggaacc tgagagcctt 8640
cacggaggcc atgaccaggt actctgcccc tcctggtgat ccccccagac cggaatatga 8700
cctggagcta ataacatcct gttcctcaaa tgtgtctgtg gcgttgggcc cgcggggccg 8760
ccgcagatac tacctgacca gagacccaac cactccactc gcccgggctg cctgggaaac 8820
agttagacac tcccctatca attcatggct gggaaacatc atccagtatg ctccaaccat 8880
atgggttcgc atggtcctaa tgacacactt cttctccatt ctcatggtcc aagacaccct 8940
ggaccagaac ctcaactttg agatgtatgg atcagtatac tccgtgaatc ctttggacct 9000
tccagccata attgagaggt tacacgggct tgacgccttt tctatgcaca catactctca 9060
ccacgaactg acgcgggtgg cttcagccct cagaaaactt ggggcgccac ccctcagggt 9120
gtggaagagt cgggctcgcg cagtcagggc gtccctcatc tcccgtggag ggaaagcggc 9180
cgtttgcggc cgatatctct tcaattgggc ggtgaagacc aagctcaaac tcactccatt 9240
gccggaggcg cgcctactgg acttatccag ttggttcacc gtcggcgccg gcgggggcga 9300
catttttcac agcgtgtcgc gcgcccgacc ccgctcatta ctcttcggcc tactcctact 9360
tttcgtaggg gtaggcctct tcctactccc cgctcggtag agcggcacac actaggtaca 9420
ctccatagct aactgttcct tttttttttt tttttttttt tttttttttt tttttttttt 9480
ttcttttttt tttttttccc tctttcttcc cttctcatct tattctactt tctttcttgg 9540
tggctccatc ttagccctag tcacggctag ctgtgaaagg tccgtgagcc gcatgactgc 9600
agagagtgcc gtaactggtc tctctgcaga tcatgt 9636
<210> 8
<211> 9636
<212> DNA
<213> 丙型肝炎病毒CH6aORF_22m(Hepatitis C virus)
<400> 8
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
cgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatctggt ggccactggg ggatgctact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcatca actccagtgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgt 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcataag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt gtcaccacct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta tttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtgggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgcgta tgcacagcag acgaggggcc tagtcggcac 3480
cattgtgacc agcctaaccg gacgtgacaa aaatgaggtc gaaggggagg tacaggtggt 3540
ctccacggct acccaatcct tcctggcgac ctccattaac ggtgtcatgt ggaccgttta 3600
tcatggggcc ggttcaaaga ctctcgccgg accgaaagga ccagtgtgtc aaatgtacac 3660
caacgtggac aaggacctag tagggtggcc atctccccag ggagcaagat ccctcacccc 3720
atgtacttgt ggctctagtg acctctattt ggtcacgagg gaggccgacg tcatccccgc 3780
aagacgcagg ggtgacagcc gtgctgccct cctctctcct aggcccataa gcaccttgaa 3840
aggctcttcg ggaggcccca ttatgtgtcc ctcggggcac gttgtgggac tcttccgagc 3900
agccgtatgc acaaggggtg tagcaaaatc cttagatttt atcccagtgg aaaacatgga 3960
gacgactatg cgctctccct cattcacaga caattccaca ccccctgcgg tgccccagac 4020
ctatcaggtg gggtacctgc acgcaccaac aggcagcgga aagagcaccc gtgtcccggc 4080
ggcgtacgct agccagggct acaaggtgtt ggtcttgaac ccatcggtgg cggcgacgct 4140
tagctttggc tcgtacatga ggcaagctta cggcgtggag ccgaatgtcc gggctggggt 4200
caggactata actacagggg gcgccatcac atactccaca tatgggagat tcttggccga 4260
tgggggatgt tccggagggg cgtatgacat catcatctgc gatgagtgcc actctacaga 4320
ccctacgacg gtgttgggca ttggcacggt tctcgaccag gctgagaccg ccggggttcg 4380
tcttaccgtg cttgcaacag caacgccgcc gggttctgtc acagtacccc atcctaacat 4440
aacagagata gccctcccaa ctacgggaga aataccattt tatggaaagg gcatccctct 4500
tgagtacatc aaagggggaa gacacctcat attctgtcac tcgaagaaga agtgcgacga 4560
gctggccggg aaactgaagt cactcggctt aaacgccgtc gcattctaca gaggtgtcga 4620
cgtgtccgtt atccccactt cgggcgacgt cgtcgtctgc gcgacagacg cccttatgac 4680
cggctacaca ggcgatttcg attccgtcat cgactgtaac gtagccgtga cacaggtggt 4740
ggatctcagc ttggacccga cattttccat agagactacc accgtccctc aggatgcggt 4800
atcacggagc caacgacgag gccgcacggg gcggggtaaa ccgggggtgt acaggtttgt 4860
ctcccagggg gagaggcctt cgggtatgtt cgacaccgtc gtcctgtgtg aggcttatga 4920
cacgggatgc gcgtggtacg aactaacccc ttctgaaaca actgtcaggc tgagggccta 4980
tctgaacact cctggccttc ccttatgcca agaccacctg gaattttggg aaggcgtgtt 5040
tactggcttg acccacatag acgcccactt tctgtctcag acgaagcagg ggggtgagaa 5100
cttcgcgtac ctcgtggcat accaggctac agtatgcgcc agggccaaag cccccccgcc 5160
ttcttgggat acgatgtgga agtgtctcat cagacttaaa cccaccctta ccggccccac 5220
tccacttttg tatcggctgg gggccgtaca aaatgaggta ataacaaccc accccataac 5280
caaatacatc atgacctgca tgtctgcgga tttggaggtc atcaccagca cgtgggtcct 5340
tgtgggtgga gtcctagccg cgctcgcatc ctactgcttg tcagtgggtt gtgttgtcat 5400
ctgtggcagg ataaccttga ctggcaggcc tgctgttgtc cctgatcgtg agatcttgta 5460
ccagcaattt gacgagatgg aagagtgctc taggcacttc ccctacctcg ccgagggcca 5520
gcagatcgcc gagcaattca gacaaaaggt gttgggactc cttcaagcga gcgccaagca 5580
ggcagaagaa ctgaagcctg ctgtccattc cgcgtggcct agggtggagg agttttggag 5640
gaagcacatg tggaactttg tcagcgggat ccagtatttg gcgggtttat ccaccctgcc 5700
cggcaaccca gccgtggcat caatgatgtc atttacagcg tcgctgacca gtcctctgag 5760
gacttctcag accctgctcc tcaacatact cggcggctgg atagccaccc aagtggctcc 5820
cccccccgcg tccacagctt ttgtcgtgag cggtctagca ggagccacgg ttggaagcat 5880
cgggctcggg agggtgttgg ttgatgtgct tgccggatac ggggccggtg tgtcgggtgc 5940
tctagtcgct ttcaagatca tgagcggcga gtgcccaacc accgaagaca tggtcaatct 6000
gctacccgcg ctgttgtcgc caggggctct cgtggtggga gtcgtgtgtg ctgccatctt 6060
aagacgccac gtaggccctg cggagggtgc caatcagtgg atgaacaggc taatagcctt 6120
tgcatcaaga ggcaaccatg tgtccccgac gcactacgtg cctgagactg acgcgtcaaa 6180
aaatgtgact cagatactca cttctcttac catcaccagc ctactccgca ggttacatca 6240
gtgggtcaat gaggacacgg ccaccccttg cgctacctca tggttacgcg acgtgtggga 6300
ctgggtgtgc acagtgctat ctgactttaa agtatggttg caagccaaac ttttccctcg 6360
cctgccgggg atccccttcc tctcgtgcca aacgggatat aggggagtct gggcagggga 6420
cggggtgtgc cacaccactt gtacctgtgg ggccgtgata gctggacacg tcaaaaatgg 6480
ctccatgaaa atcacaggac ccaagacatg cagcaacact tggcacggga ctttcccaat 6540
caacgccacc actaccggcc ccagcacacc acgaccagcc ctcaactatc agcgcgctct 6600
ttggcgggtg tccgccgagg actacgttga ggtgcggagg ttgggcgact gccactacgt 6660
ggtaggggtc actgctgacg ggttgaaatg cccttgccag gtacctgcac ctgaattctt 6720
cactgaggtc gatggcgtga ggatacaccg ttacgcgcca ccttgcaagc ccttgctcag 6780
ggacgaagtg actttctctg tgggtctttc aaactatgtc atagggtctc agctcccttg 6840
cgagccagag cctgacgtga ccgtggtcac ctcaatgctc acagaccccg tgcacatcac 6900
cgcagagacg gcagcacggc ggttgaagag ggggtccccc ccctctttag ccagctcatc 6960
ggccagccag ctgtctgcac cgtccctcaa ggctacttgc acaacctcca gagaccaccc 7020
ggacatggag ctcatcgagg ccaacctcct ctggaggcag gagatgggag gcaacatcac 7080
tcgagtcgag tcagagaaca aagtcgtagt ccttgactcc tttgagcctc taaccgctga 7140
gtatgacgag agggaaatct cagtgtcagc tgagtgccac aagccaccca ggcccaaatt 7200
ccctccagct cttccagtat gggccaggcc cgactacaat ccacctctcc tacaagcatg 7260
gcaaatgccc ggatacgagc ctccagtcgt gtctggatgt gccgtcgctc cacctaaacc 7320
ggcaccaatt cctccgccga ggcggaagag gctagtgcac ttggatgagt ccacggtctc 7380
gcacgccttg gcgcagcttg ccgacaaggt atgtgtggag agtggtagtg acccgggacc 7440
tagttcagac tcgggactat caatagccag tcctgtcccg cctgtctcaa caacaccgga 7500
tgccgcctgc tcaggagcag agtcctatag ctcaatgccc cctcttgagg gagagcctgg 7560
tgaccctgac ctgagctcag gctcttggtc cactgtgagc gatcagggtg atgtcgtgtg 7620
ttgttccatg tcctattcct ggacgggggc tctaataaca ccatgcgctg cggaggagga 7680
gaagcttcca ataaatccta tgagcaactc ccttataaga caccataaca tggtgtattc 7740
caccacatca cgcagcgcca gcctccgcca gaagaaggtc acatttgaca gactgcaagt 7800
gtttgaccaa cactaccagg atgtactaaa ggagattaag cttcgagcgt ccacggtgca 7860
ggcaaaactc ctatccatag aggaagcctg tgaccttacg ccatcgcact cagcacggtc 7920
caagtatgga tatggtgcgc aggacgttag aagccatgct agcaaggccg ttgaccacat 7980
ccgctccgtg tgggaggact tgctagaaga ctctgatacc ccaattccca cgaccatcat 8040
ggctaagaat gaagtcttct gcgtagatcc gtcgaagggt ggacgcaagc cggcacgcct 8100
aatagttttc ccagacttga gcgtgcgggt ctgcgagaag atggccctat acgacgtaac 8160
gcggaagttg ccacaggccg tgatgggttc agcatacgga ttccagtact cccctaacca 8220
gagggttgag tacttgctca aaatgtggcg gtcaaagaaa gtgcccatgg gcttttctta 8280
cgacaccagg tgttttgatt caaccgtcac tgagcgggat atccggactg agaacgaaat 8340
ctatcagtct tgccagctgg atcccgtggc aaggagggca gtatcatccc taacggagcg 8400
gctctacgta ggcggcccca tggtaaactc caagggacag ccatgtggct accgtagatg 8460
ccgcgccagt ggggtgctgc ccacgagcat gggaaacacc ctcacatgct atctgaaggc 8520
acaggccgcc tgcagggcgg ccaacatcaa agactatgac atgttggtgt gcggagatga 8580
cttagtggtc atttgtgaga gtgctggcgt ccaggaggac actgcgtcac tgcgagcatt 8640
cacggatgct atgaccaggt attcagctcc ccctggagac gccccgcaac ctacatatga 8700
ccttgagctc ataacatcat gctcatccaa cgtctccgtc gcccatgacg gcaacgggaa 8760
gagatattac tacctcacac gtgactgcac tactccactt gcgcgggctg catgggagac 8820
agcccgccac actccagtca attcgtggtt gggcaacatc attatgtttg cccccacgat 8880
atgggtgcgt atggttctga tgacccattt tttctccata ctccagtcgc aagaacaatt 8940
ggagaaagca ctcgactttg acatctacgg agtgacctac tccgtctctc cacttgatct 9000
cccagcaatc attcaacgac tccatggcat ggcagcattt tcactccacg gatattctcc 9060
agttgagctc aatagggtag gggcttgcct caggaaactt ggggtacctc ccttgagagc 9120
ctggagacat cgagccagag ctgtcagagc caaactcatt gcccaggggg ggaaagcggc 9180
tatatgcggt aagtacctct ttaactgggc ggtgaagacc aaattaaaac tcactccact 9240
ggtctccgcg agcaagcttg acttatcagg ctggttcgtg gcaggctacg acgggggggg 9300
catttttcac agcgtgtccc aggctcgacc ccgtctctta ctccttggcc tactcctact 9360
ctcagtaggg gtaggcatct ttttgctccc cgctcggtag agcggcacac actaggtaca 9420
ctccatagct aactgttcct tttttttttt tttttttttt tttttttttt tttttttttt 9480
ttcttttttt tttttttccc tctttcttcc cttctcatct tattctactt tctttcttgg 9540
tggctccatc ttagccctag tcacggctag ctgtgaaagg tccgtgagcc gcatgactgc 9600
agagagtgcc gtaactggtc tctctgcaga tcatgt 9636
<210> 9
<211> 9636
<212> DNA
<213> 丙型肝炎病毒CH6aORF_24m(Hepatitis C virus)
<400> 9
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
cgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatctggt ggccactggg ggatgctact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcatca actccagtgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgt 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcataag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt gtcaccacct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta tttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtgggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgcgta tgcacagcag acgaggggcc tagtcggcac 3480
cattgtgacc agcctaaccg gacgtgacaa aaatgaggtc gaaggggagg tacaggtggt 3540
ctccacggct acccaatcct tcctggcgac ctccattaac ggtgtcatgt ggaccgttta 3600
tcatggggcc ggttcaaaga ctctcgccgg accgaaagga ccagtgtgtc aaatgtacac 3660
caacgtggac aaggacctag tagggtggcc atctcccaag ggagcaagat ccctcacccc 3720
atgtacttgt ggctctagtg acctctattt ggtcacgagg gaggccgacg tcatccccgc 3780
aagacgcagg ggtgacagcc gtgctgccct cctctctcct aggcccataa gcaccttgaa 3840
aggctcttcg ggaggcccca ttatgtgtcc ctcggggcac gttgtgggac tcttccgagc 3900
agccgtatgc acaaggggtg tagcaaaatc cttagatttt atcccagtgg aaaacatgga 3960
gacgactatg cgctctccct cattcacaga caattccaca ccccctgcgg tgccccagac 4020
ctatcaggtg gggtacctgc acgcaccaac aggcagcgga aagagcaccc gtgtcccggc 4080
ggcgtacgct agccagggct acaaggtgtt ggtcttgaac ccatcggtgg cggcgacgct 4140
tagctttggc tcgtacatga ggcaagctta cggcgtggag ccgaatgtcc gggctggggt 4200
caggactata actacagggg gcgccatcac atactccaca tatgggagat tcttggccga 4260
tgggggatgt tccggagggg cgtatgacat catcatctgc gatgagtgcc actctacaga 4320
ccctacgacg gtgttgggca ttggcacggt tctcgaccag gctgagaccg ccggggttcg 4380
tcttaccgtg cttgcaacag caacgccgcc gggttctgtc acagtacccc atcctaacat 4440
aacagagata gccctcccaa ctacgggaga aataccattt tatggaaagg gcatccctct 4500
tgagtacatc aaagggggaa gacacctcat attctgtcac tcgaagaaga agtgcgacga 4560
gctggccggg aaactgaagt cactcggctt aaacgccgtc gcattctaca gaggtgtcga 4620
cgtgtccgtt atccccactt cgggcgacgt cgtcgtctgc gcgacagacg cccttatgac 4680
cggctacaca ggcgatttcg attccgtcat cgactgtaac gtagccgtga cacaggtggt 4740
ggatctcagc ttggacccga cattttccat agagactacc accgtccctc aggatgcggt 4800
atcacggagc caacgacgag gccgcacggg gcggggtaaa ccgggggtgt acaggtttgt 4860
ctcccagggg gagaggcctt cgggtatgtt cgacaccgtc gtcctgtgtg aggcttatga 4920
cacgggatgc gcgtggtacg aactaacccc ttctgaaaca actgtcaggc tgagggccta 4980
tctgaacact cctggccttc ccttatgcca agaccacctg gaattttggg aaggcgtgtt 5040
tactggcttg acccacatag acgcccactt tctgtctcag acgaagcagg ggggtgagaa 5100
cttcgcgtac ctcgtggcat accaggctac agtatgcgcc agggccaaag cccccccgcc 5160
ttcttgggat acgatgtgga agtgtctcat cagacttaaa cccaccctta ccggccccac 5220
tccacttttg tatcggctgg gggccgtaca aaatgaggta ataacaaccc accccataac 5280
ccaatacatc atgacctgca tgtctgcgga tttggaggtc atcaccagca cgtgggtcct 5340
tgtgggtgga gtcctagccg cgctcgcatc ctactgcttg tcagtgggtt gtgttgtcat 5400
ctgtggcagg ataaccttga ctggcaggcc tgctgttgtc cctgatcgtg agatcttgta 5460
ccagcaattt gacgagatgg aagagtgctc taggcacttc ccctacctcg ccgagggcca 5520
gcagatcgcc gagcaattca gacaaaaggt gttgggactc cttcaagcga gcgccaagca 5580
ggcagaagaa ctgaagcctg ctgtccattc cgcgtggcct agggtggagg agttttggag 5640
gaagcacatg tggaactttg tcagcgggat ccagtatttg gcgggtttat ccaccctgcc 5700
cggcaaccca gccgtggcat caatgatgtc atttacagcg tcgctgacca gtcctctgag 5760
gacttctcag accctgctcc tcaacatact cggcggctgg atagccaccc aagtggctcc 5820
cccccccgcg tccacagctt ttgtcgtgag cggtctagca ggagccacgg ttggaagcat 5880
cgggctcggg agggtgttgg ttgatgtgct tgccggatac ggggccggtg tgtcgggtgc 5940
tctagtcgct ttcaagatca tgagcggcga gtgcccaacc accgaagaca tggtcaatct 6000
gctacccgcg ctgttgtcgc caggggctct cgtggtggga gtcgtgtgtg ctgccatctt 6060
aagacgccac gtaggccctg cggagggtgc caatcagtgg atgaacaggc taatagcctt 6120
tgcatcaaga ggcaaccatg tgtccccgac gcactacgtg cctgagactg acgcgtcaaa 6180
aaatgtgact cagatactca cttctcttac catcaccagc ctactccgca ggttacatca 6240
gtgggtcaat gaggacacgg ccaccccttg cgctacctca tggttacgcg acgtgtggga 6300
ctgggtgtgc acagtgctat ctgactttaa agtatggttg caagccaaac ttttccctcg 6360
cctgccgggg atccccttcc tctcgtgcca aacgggatat aggggagtct gggcagggga 6420
cggggtgtgc cacaccactt gtacctgtgg ggccgtgata gctggacacg tcaaaaatgg 6480
ctccatgaaa atcacaggac ccaagacatg cagcaacact tggcacggga ctttcccaat 6540
caacgccacc actaccggcc ccagcacacc acgaccagcc ctcaactatc agcgcgctct 6600
ttggcgggtg tccgccgagg actacgttga ggtgcggagg ttgggcgact gccactacgt 6660
ggtaggggtc actgctgacg ggttgaaatg cccttgccag gtacctgcac ctgaattctt 6720
cactgaggtc gatggcgtga ggatacaccg ttacgcgcca ccttgcaagc ccttgctcag 6780
ggacgaagtg actttctctg tgggtctttc aaactatgtc atagggtctc agctcccttg 6840
cgagccagag cctgacgtga ccgtggtcac ctcaatgctc acagaccccg tgcacatcac 6900
cgcagagacg gcagcacggc ggttgaagag ggggtccccc ccctctttag ccagctcatc 6960
ggccagccag ctgtctgcac cgtccctcaa ggctacttgc acaacctcca gagaccaccc 7020
ggacatggag ctcatcgagg ccaacctcct ctggaggcag gagatgggag gcaacatcac 7080
tcgagtcgag tcagagaaca aagtcgtagt ccttgactcc tttgagcctc taaccgctga 7140
gtatgacgag agggaaatct cagtgtcagc tgagtgccac aagccaccca ggcccaaatt 7200
ccctccagct cttccagtat gggccaggcc cgactacaat ccacctctcc tacaagcatg 7260
gcaaatgccc ggatacgagc ctccagtcgt gtctggatgt gccgtcgctc cacctaaacc 7320
ggcaccaatt cctccgccga ggcggaagag gctagtgcac ttggatgagt ccacggtctc 7380
gcacgccttg gcgcagcttg ccgacaaggt atgtgtggag agtggtagtg acccgggacc 7440
tagttcagac tcgggactat caatagccag tcctgtcccg cctgtctcaa caacaccgga 7500
tgccgcctgc tcaggagcag agtcctatag ctcaatgccc cctcttgagg gagagcctgg 7560
tgaccctgac ctgagctcag gctcttggtc cactgtgagc gatcagggtg atgtcgtgtg 7620
ttgttccatg tcctattcct ggacgggggc tctaataaca ccatgcgctg cggaggagga 7680
gaagcttcca ataaatccta tgagcaactc ccttataaga caccataaca tggtgtattc 7740
caccacatca cgcagcgcca gcctccgcca gaagaaggtc acatttgaca gactgcaagt 7800
gtttgaccaa cactaccagg atgtactaaa ggagattaag cttcgagcgt ccacggtgca 7860
ggcaaaactc ctatccatag aggaagcctg tgaccttacg ccatcgcact cagcacggtc 7920
caagtatgga tatggtgcgc aggacgttag aagccatgct agcaaggccg ttgaccacat 7980
ccgctccgtg tgggaggact tgctagaaga ctctgatacc ccaattccca cgaccatcat 8040
ggctaagaat gaagtcttct gcgtagatcc gtcgaagggt ggacgcaagc cggcacgcct 8100
aatagttttc ccagacttga gcgtgcgggt ctgcgagaag atggccctat acgacgtaac 8160
gcggaagttg ccacaggccg tgatgggttc agcatacgga ttccagtact cccctaacca 8220
gagggttgag tacttgctca aaatgtggcg gtcaaagaaa gtgcccatgg gcttttctta 8280
cgacaccagg tgttttgatt caaccgtcac tgagcgggat atccggactg agaacgaaat 8340
ctatcagtct tgccagctgg atcccgtggc aaggagggca gtatcatccc taacggagcg 8400
gctctacgta ggcggcccca tggtaaactc caagggacag ccatgtggct accgtagatg 8460
ccgcgccagt ggggtgctgc ccacgagcat gggaaacacc ctcacatgct atctgaaggc 8520
acaggccgcc tgcagggcgg ccaacatcaa agactatgac atgttggtgt gcggagatga 8580
cttagtggtc atttgtgaga gtgctggcgt ccaggaggac actgcgtcac tgcgagcatt 8640
cacggatgct atgaccaggt attcagctcc ccctggagac gccccgcaac ctacatatga 8700
ccttgagctc ataacatcat gctcatccaa cgtctccgtc gcccatgacg gcaacgggaa 8760
gagatattac tacctcacac gtgactgcac tactccactt gcgcgggctg catgggagac 8820
agcccgccac actccagtca attcgtggtt gggcaacatc attatgtttg cccccacgat 8880
atgggtgcgt atggttctga tgacccattt tttctccata ctccagtcgc aagaacaatt 8940
ggagaaagca ctcgactttg acatctacgg agtgacctac tccgtctctc cacttgatct 9000
cccagcaatc attcaacgac tccatggcat ggcagcattt tcactccacg gatattctcc 9060
agttgagctc aatagggtag gggcttgcct caggaaactt ggggtacctc ccttgagagc 9120
ctggagacat cgagccagag ctgtcagagc caaactcatt gcccaggggg ggaaagcggc 9180
tatatgcggt aagtacctct ttaactgggc ggtgaagacc aaattaaaac tcactccact 9240
ggtctccgcg agcaagcttg acttatcagg ctggttcgtg gcaggctacg acgggggggg 9300
catttttcac agcgtgtccc aggctcgacc ccgtctctta ctccttggcc tactcctact 9360
ctcagtaggg gtaggcatct ttttgctccc cgctcggtag agcggcacac actaggtaca 9420
ctccatagct aactgttcct tttttttttt tttttttttt tttttttttt tttttttttt 9480
ttcttttttt tttttttccc tctttcttcc cttctcatct tattctactt tctttcttgg 9540
tggctccatc ttagccctag tcacggctag ctgtgaaagg tccgtgagcc gcatgactgc 9600
agagagtgcc gtaactggtc tctctgcaga tcatgt 9636
<210> 10
<211> 9636
<212> DNA
<213> 丙型肝炎病毒CH6aORF_25m(Hepatitis C virus)
<400> 10
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
cgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatctggt ggccactggg ggatgctact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcatca actccagtgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgt 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcataag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt gtcaccacct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta tttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtgggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgcgta tgcacagcag acgaggggcc tagtcggcac 3480
cattgtgacc agcctaaccg gacgtgacaa aaatgaggtc gaaggggagg tacaggtggt 3540
ctccacggct acccaatcct tcctggcgac ctccattaac ggtgtcatgt ggaccgttta 3600
tcatggggcc ggttcaaaga ctctcgccgg accgaaagga ccagtgtgtc aaatgtacac 3660
caacgtggac aaggacctag tagggtggcc atctcccaag ggagcaagat ccctcacccc 3720
atgtacttgt ggctctagtg acctctattt ggtcacgagg gaggccgacg tcatccccgc 3780
aagacgcagg ggtgacagcc gtgctgccct cctctctcct aggcccataa gcaccttgaa 3840
aggctcttcg ggaggcccca ttatgtgtcc ctcggggcac gttgtgggac tcttccgagc 3900
agccgtatgc acaaggggtg tagcaaaatc cttagatttt atcccagtgg aaaacatgga 3960
gacgactatg cgctctccct cattcacaga caattccaca ccccctgcgg tgccccagac 4020
ctatcaggtg gggtacctgc acgcaccaac aggcagcgga aagagcaccc gtgtcccggc 4080
ggcgtacgct agccagggct acaaggtgtt ggtcttgaac ccatcggtgg cggcgacgct 4140
tagctttggc tcgtacatga ggcaagctta cggcgtggag ccgaatgtcc gggctggggt 4200
caggactata actacagggg gcgccatcac atactccaca tatgggagat tcttggccga 4260
tgggggatgt tccggagggg cgtatgacat catcatctgc gatgagtgcc actctacaga 4320
ccctacgacg gtgttgggca ttggcacggt tctcgaccag gctgagaccg ccggggttcg 4380
tcttaccgtg cttgcaacag caacgccgcc gggttctgtc acagtacccc atcctaacat 4440
aacagagata gccctcccaa ctacgggaga aataccattt tatggaaagg gcatccctct 4500
tgagtacatc aaagggggaa gacacctcat attctgtcac tcgaagaaga agtgcgacga 4560
gctggccggg aaactgaagt cactcggctt aaacgccgtc gcattctaca gaggtgtcga 4620
cgtgtccgtt atccccactt cgggcgacgt cgtcgtctgc gcgacagacg cccttatgac 4680
cggctacaca ggcgatttcg attccgtcat cgactgtaac gtagccgtga cacaggtggt 4740
ggatctcagc ttggacccga cattttccat agagactacc accgtccctc aggatgcggt 4800
atcacggagc caacgacgag gccgcacggg gcggggtaaa ccgggggtgt acaggtttgt 4860
ctcccagggg gagaggcctt cgggtatgtt cgacaccgtc gtcctgtgtg aggcttatga 4920
cacgggatgc gcgtggtacg aactaacccc ttctgaaaca actgtcaggc tgagggccta 4980
tctgaacact cctggccttc ccttatgcca agaccacctg gaattttggg aaggcgtgtt 5040
tactggcttg acccacatag acgcccactt tctgtctcag acgaagcagg ggggtgagaa 5100
cttcgcgtac ctcgtggcat accaggctac agtatgcgcc agggccaaag cccccccgcc 5160
ttcttgggat acgatgtgga agtgtctcat cagacttaaa cccaccctta ccggccccac 5220
tccacttttg tatcggctgg gggccgtaca aaatgaggta ataacaaccc accccataac 5280
ccaatacatc atgacctgca tgtctgcgga tttggaggtc atcaccagca cgtgggtcct 5340
tgtgggtgga gtcctagccg cgctcgcatc ctactgcttg tcagtgggtt gtgttgtcat 5400
ctgtggcagg ataaccttga ctggcaggcc tgctgttgtc cctgatcgtg agatcttgta 5460
ccagcaattt gacgagatgg aagagtgctc taggcacttc ccctacctcg ccgagggcca 5520
gcagatcgcc gagcaattca gacaaaaggt gttgggactc cttcaagcga gcgccaagca 5580
ggcagaagaa ctgaagcctg ctgtccattc cgcgtggcct agggtggagg agttttggag 5640
gaagcacatg tggaactttg tcagcgggat ccagtatttg gcgggtttat ccaccctgcc 5700
cggcaaccca gccgtggcat caatgatgtc atttacagcg tcgctgacca gtcctctgag 5760
gacttctcag accctgctcc tcaacatact cggcggctgg atagccaccc aagtggctcc 5820
cccccccgcg tccacagctt ttgtcgtgag cggtctagca ggagccacgg ttggaagcat 5880
cgggctcggg agggtgttgg ttgatgtgct tgccggatac ggggccggtg tgtcgggtgc 5940
tctagtcgct ttcaagatca tgagcggcga gtgcccaacc accgaagaca tggtcaatct 6000
gctacccgcg ctgttgtcgc caggggctct cgtggtggga gtcgtgtgtg ctgccatctt 6060
aagacgccac gtaggccctg cggagggtgc caatcagtgg atgaacaggc taatagcctt 6120
tgcatcaaga ggcaaccatg tgtccccgac gcactacgtg cctgagactg acgcgtcaaa 6180
aaatgtgact cagatactca cttctcttac catcaccagc ctactccgca ggttacatca 6240
gtgggtcaat gaggacacgg ccaccccttg cgctacctca tggttacgcg acgtgtggga 6300
ctgggtgtgc acagtgctat ctgactttaa agtatggttg caagccaaac ttttccctcg 6360
cctgccgggg atccccttcc tctcgtgcca aacgggatat aggggagtct gggcagggga 6420
cggggtgtgc cacaccactt gtacctgtgg ggccgtgata gctggacacg tcaaaaatgg 6480
ctccatgaaa atcacaggac ccaagacatg cagcaacact tggcacggga ctttcccaat 6540
caacgccacc actaccggcc ccagcacacc acgaccagcc ctcaactatc agcgcgctct 6600
ttggcgggtg tccgccgagg actacgttga ggtgcggagg ttgggcgact gccactacgt 6660
ggtaggggtc actgctgacg ggttgaaatg cccttgccag gtacctgcac ctgaattctt 6720
cactgaggtc gatggcgtga ggatacaccg ttacgcgcca ccttgcaagc ccttgctcag 6780
ggacgaagtg actttctctg tgggtctttc aaactatgtc atagggtctc agctcccttg 6840
cgagccagag cctgacgtga ccgtggtcac ctcaatgctc acagaccccg tgcacatcac 6900
cgcagagacg gcagcacggc ggttgaagag ggggtccccc ccctctttag ccagctcatc 6960
ggccagccag ctgtctgcac cgtccctcaa ggctacttgc acaacctcca gagaccaccc 7020
ggacatggag ctcatcgagg ccaacctcct ctggaggcag gagatgggag gcaacatcac 7080
tcgagtcgag tcagagaaca aagtcgtagt ccttgactcc tttgagcctc taaccgctga 7140
gtatgacgag agggaaatct cagtgtcagc tgagtgccac aagccaccca ggcccaaatt 7200
ccctccagct cttccagtat gggccaggcc cgactacaat ccacctctcc tacaagcatg 7260
gcaaatgccc ggatacgagc ctccagtcgt gtctggatgt gccgtcgctc cacctaaacc 7320
ggcaccaatt cctccgccga ggcggaagag gctagtgcac ttggatgagt ccacggtctc 7380
gcacgccttg gcgcagcttg ccgacaaggt atgtgtggag agtggtagtg acccgggacc 7440
tagttcagac tcgggactat caatagccag tcctgtcccg cctgtctcaa caacaccgga 7500
tgccgcctgc tcaggagcag agtcctatag ctcaatgccc cctcttgagg gagagcctgg 7560
tgaccctgac ctgagctcag gctcttggtc cactgtgagc gatcagggtg atgtcgtgtg 7620
ttgttccatg tcctattcct ggacgggggc tctaataaca ccatgcgctg cggaggagga 7680
gaagcttcca ataaatccta tgagcaactc ccttataaga caccataaca tggtgtattc 7740
caccacatca cgcagcgcca gcctccgcca gaagaaggtc acatttgaca gactgcaagt 7800
gtttgaccaa cactaccagg atgtactaaa ggagattaag cttcgagcgt ccacggtgca 7860
ggcaaaactc ctatccatag aggaagcctg tgaccttacg ccatcgcact cagcacggtc 7920
caagtatgga tatggtgcgc aggacgttag aagccatgct agcaaggccg ttgaccacat 7980
ccgctccgtg tgggaggact tgctagaaga ctctgatacc ccaattccca cgaccatcat 8040
ggctaagaat gaagtcttct gcgtagatcc gtcgaagggt ggacgcaagc cggcacgcct 8100
aatagttttc ccagacttga gcgtgcgggt ctgcgagaag atggccctat acgacgtaac 8160
gcggaagttg ccacaggccg tgatgggttc agcatacgga ttccagtact cccctaacca 8220
gagggttgag tacttgctca aaatgtggcg gtcaaagaaa gtgcccatgg gcttttctta 8280
cgacaccagg tgttttgatt caaccgtcac tgagcgggat atccggactg agaacgaaat 8340
ctatcagtct tgccagctgg atcccgtggc aaggagggca gtatcatccc taacggagcg 8400
gctctacgta ggcggcccca tggtaaactc caagggacag ccatgtggct accgtagatg 8460
ccgcgccagt ggggtgctgc ccacgagcat gggaaacacc ctcacatgct atctgaaggc 8520
acaggccgcc tgcagggcgg ccaacatcaa agactatgac atgttggtgt gcggagatga 8580
cttagtggtc atttgtgaga gtgctggcgt ccaggaggac actgcgtcac tgcgagcatt 8640
cacggatgct atgaccaggt attcagctcc ccctggagac gccccgcaac ctacatatga 8700
ccttgagctc ataacatcat gctcatccaa cgtctccgtc gcccatgacg gcaacgggaa 8760
gagatattac tacctcacac gtgactgcac tactccactt gcgcgggctg catgggagac 8820
agcccgccac actccagtca attcgtggtt gggcaacatc attatgtttg cccccacgat 8880
atgggtgcgt atggttctga tgacccattt tttctccata ctccagtcgc aagaacaatt 8940
ggagaaagca ctcgactttg acatctacgg agtgacctac tccgtctctc cacttgatct 9000
cccagcaatc attcaacgac tccatggcat ggcagcattt tcactccacg gatattctcc 9060
agttgagctc aatagggtag gggcttgcct caggaaactt ggggtacctc ccttgagagc 9120
ctggagacat cgagccagag ctgtcagagc caaactcatt gtccaggggg ggaaagcggc 9180
tatatgcggt aagtacctct ttaactgggc ggtgaagacc aaattaaaac tcactccact 9240
ggtctccgcg agcaagcttg acttatcagg ctggttcgtg gcaggctacg acgggggggg 9300
catttttcac agcgtgtccc aggctcgacc ccgtctctta ctccttggcc tactcctact 9360
ctcagtaggg gtaggcatct ttttgctccc cgctcggtag agcggcacac actaggtaca 9420
ctccatagct aactgttcct tttttttttt tttttttttt tttttttttt tttttttttt 9480
ttcttttttt tttttttccc tctttcttcc cttctcatct tattctactt tctttcttgg 9540
tggctccatc ttagccctag tcacggctag ctgtgaaagg tccgtgagcc gcatgactgc 9600
agagagtgcc gtaactggtc tctctgcaga tcatgt 9636
<210> 11
<211> 9636
<212> DNA
<213> 丙型肝炎病毒CH6aORF_26m(Hepatitis C virus)
<400> 11
acctgcccct aataggggcg acactccgcc atgaatcact cccctgtgag gaactactgt 60
cttcacgcag aaagcgccta gccatggcgt tagtatgagt gtcgtacagc ctccaggccc 120
ccccctcccg ggagagccat agtggtctgc ggaaccggtg agtacaccgg aattgccggg 180
aagactgggt cctttcttgg ataaacccac tctatgcccg gccatttggg cgtgcccccg 240
caagactgct agccgagtag cgttgggttg cgaaaggcct tgtggtactg cctgataggg 300
cgcttgcgag tgccccggga ggtctcgtag accgtgcacc atgagcacac ttccaaaacc 360
ccaaagaaaa accaaaagga acaccaaccg tcgcccaatg gacgtcaagt tcccgggtgg 420
cggtcagatc gttggcggag tttacttgtt gccgcgcagg ggcccccggt tgggtgtgcg 480
cgcgacgagg aagacttccg agcgatccca gcccagaggc aggcgccaac ctataccaaa 540
ggcgcgccag ccccagggca ggcactgggc tcagcccgga tacccttggc ccctttatgg 600
aaacgagggc tgcgggtggg caggttggct cctgtccccc cgcggctccc ggccgcactg 660
gggccccaac gacccccggc gtcgatcccg caatctgggt aaggtcatcg ataccctaac 720
gtgtgggttc gccgatctca tggggtacat ccccgtcgtg ggcgcgcctt tgggcggcgt 780
cgcggctgcg ctcgcacacg gtgtgagggc aatcgaggac gggatcaatt atgcaacagg 840
aaatcttccc ggttgctctt tctctatctt cctcttggca ctactctcgt gcctaacaac 900
gccagcttcg gctcttacct acggcaactc cagtgaccta taccatctca caaatgattg 960
ccccaactcc agcatcgtgc tggaggcgga tgccatgatc ttgcatttgc ctggatgctt 1020
gccttgtgta aaggtcgata ataagtccac ctgctggcat gctgtgtccc ccaccctagc 1080
cataccaaat gcttccacgc ccgcaacggg gttccgtagg cacgtggatc ttcttgcggg 1140
cgctgcggtg gtctgttcat ctctgtatat cggggatttg tgcggttctc tcttcctagc 1200
gggacaacta ttcaccttcc agcctcgccg tcactggact gtgcaagact gcaactgttc 1260
catctataca ggccacgtca ctggccacag gatggcttgg gacatgatga tgaactggtc 1320
acccacaacc actctggtcc tatctagcat cttgagggta cctgagatct gtgcgagtgt 1380
gatatctggt ggccactggg ggatgctact agccgttgcc tactttggta tggctggtaa 1440
ctggctaaaa gtcctggctg ttctgttctt atttgcaggg gtcgaagcaa ccaccaccac 1500
cggctccgcg ataagcaaag ctacttccgg ctttaccgcg cttttcaacc ccggtgccag 1560
gcagaatcta cacctcacca actccagtgg tagctggcat ataaacagga ctgctttgaa 1620
ctgcaatgat tccctccata cggggttcat agcgtcactc ttttacgccc acaagttcaa 1680
ctcctcgggc tgcccagagc ggatggccgc atgtaagccc cttgctgact tcaagcaggg 1740
gtggggccaa ataacctaca aagtcaacat ctcaggcccc tctgacgacc gtccctactg 1800
ttggcattac gctcccaggc catgtgacgt ggtggcagcc cgcacggtgt gcggccctgt 1860
ttactgcttc acgcctagcc ctgttgtggt aggaaccact gacaggcggg gcaaccccac 1920
atacacctgg ggggagaatg agacggatgt gttcatgctg gaaagccttc ggccccctac 1980
tggggggtgg tttgggtgca cctggatgaa cgctacgggc tttaccaaga cctgtggtgc 2040
cccgccatgt cagatagtcc caggggatta caacagctct gccaatgagc ttttgtgccc 2100
caccgattgc ttccgtaaac atccgggagc cacataccag cggtgtggat caggaccctg 2160
gttaacacct aggtgtctag tggactatcc ctacagactg tggcactacc cctgcactgt 2220
aaacttcacc ttgcataagg tcaggatgtt tgtggggggc gttgagcacc ggtttgacgc 2280
cgcatgtaat tggaccaggg gcgagcggtg cgagttacat gacagagaca ggattgaaat 2340
gagcccgctg cttttctcaa ccacgcagct tgccatactt ccatgttcat tttccaccat 2400
gccggccttg tcaactggcc tcattcacct gcatcagaac atagtggacg tgcagtacct 2460
ctacggagtc tcctcgagcg tcacctcgtg ggtagtaaag tgggagtaca ttgtcctgat 2520
gtttctggtt ctggcagatg ctcggatttg tacgtgtctc tggttaatgc tgctcataag 2580
tagggttgag gcagcagtgg agaggcttgt cgtcctcaac gcggctagcg ccgccggcac 2640
tgctggctgg tggtgggcgg tgcttttcct gtgctgtgct tggtacgtga aaggtcgcct 2700
tgtgcctgcg tgtacctaca tggcgctggg aatgtggcca ttgctcttag caatcttggc 2760
cctgcctcag cgagcgtatg ccatggacaa tgagcaagcg gcatccctcg gagctgttgg 2820
tctattggtt gtcaccacct tcaccatcac ccccatgtac aagaagctat tgacctgctt 2880
catttggtgg aatcagtatt tcctcgcccg agctgaggcc atggtacacg agtgggtgcc 2940
cgacctacgg gttaggggtg gtagggattc catcatctta tttacctgct tgttccatcc 3000
acagctgggg tttgaggtca ctaagatcct actagctatc ctggcccctc tatacatctt 3060
gcagtacagt ttgctcaaag tgccttactt tgtgcgcgcc cacatactcc tgcgtgcttg 3120
cctgcttgtt cgtgggctgg cagggggtaa gtacgtgcag gcgtgccttc tgaggttggg 3180
cgcttggact ggtacctatg tttatgacca tctcgccccc ctttctggct gggctagcga 3240
cggactgcgc gatctagcag ttgcagtcga gccggtcatt ttctctccta tggagaagaa 3300
aattatcacc tggggtgcgg acactgccgc gtgtggtgac atcttgagtg gtctcccggt 3360
gtcagcgagg ttggggaatt tggtgctact aggacccgcg gatgatatgc ggcgcggagg 3420
ttggaagctt ttggctccca tcactgcgta tgcacagcag acgaggggcc tagtcggcac 3480
cattgtgacc agcctaaccg gacgtgacaa aaatgaggtc gaaggggagg tacaggtggt 3540
ctccacggct acccaatcct tcctggcgac ctccattaac ggtgtcatgt ggaccgttta 3600
tcatggggcc ggttcaaaga ctctcgccgg accgaaagga ccagtgtgtc aaatgtacac 3660
caacgtggac aaggacctag tagggtggcc atctcccaag ggagcaagat ccctcacccc 3720
atgtacttgt ggctctagtg acctctattt ggtcacgagg gaggccgacg tcatccccgc 3780
aagacgcagg ggtgacagcc gtgctgccct cctctctcct aggcccataa gcaccttgaa 3840
aggctcttcg ggaggcccca ttatgtgtcc ctcggggcac gttgtgggac tcttccgagc 3900
agccgtatgc acaaggggtg tagcaaaatc cttagatttt atcccagtgg aaaacatgga 3960
gacgactatg cgctctccct cattcacaga caattccaca ccccctgcgg tgccccagac 4020
ctatcaggtg gggtacctgc acgcaccaac aggcagcgga aagagcaccc gtgtcccggc 4080
ggcgtacgct agccagggct acaaggtgtt ggtcttgaac ccatcggtgg cggcgacgct 4140
tagctttggc tcgtacatga ggcaagctta cggcgtggag ccgaatgtcc gggctggggt 4200
caggactata actacagggg gcgccatcac atactccaca tatgggagat tcttggccga 4260
tgggggatgt tccggagggg cgtatgacat catcatctgc gatgagtgcc actctacaga 4320
ccctacgacg gtgttgggca ttggcacggt tctcgaccag gctgagaccg ccggggttcg 4380
tcttaccgtg cttgcaacag caacgccgcc gggttctgtc acagtacccc atcctaacat 4440
aacagagata gccctcccaa ctacgggaga aataccattt tatggaaagg gcatccctct 4500
tgagtacatc aaagggggaa gacacctcat attctgtcac tcgaagaaga agtgcgacga 4560
gctggccggg aaactgaagt cactcggctt aaacgccgtc gcattctaca gaggtgtcga 4620
cgtgtccgtt atccccactt cgggcgacgt cgtcgtctgc gcgacagacg cccttatgac 4680
cggctacaca ggcgatttcg attccgtcat cgactgtaac gtagccgtga cacaggtggt 4740
ggatctcagc ttggacccga cattttccat agagactacc accgtccctc aggatgcggt 4800
atcacggagc caacgacgag gccgcacggg gcggggtaaa ccgggggtgt acaggtttgt 4860
ctcccagggg gagaggcctt cgggtatgtt cgacaccgtc gtcctgtgtg aggcttatga 4920
cacgggatgc gcgtggtacg aactaacccc ttctgaaaca actgtcaggc tgagggccta 4980
tctgaacact cctggccttc ccttatgcca agaccacctg gaattttggg aaggcgtgtt 5040
tactggcttg acccacatag acgcccactt tctgtctcag acgaagcagg ggggtgagaa 5100
cttcgcgtac ctcgtggcat accaggctac agtatgcgcc agggccaaag cccccccgcc 5160
ttcttgggat acgatgtgga agtgtctcat cagacttaaa cccaccctta ccggccccac 5220
tccacttttg tatcggctgg gggccgtaca aaatgaggta ataacaaccc accccataac 5280
ccaatacatc atgacctgca tgtctgcgga tttggaggtc atcaccagca cgtgggtcct 5340
tgtgggtgga gtcctagccg cgctcgcatc ctactgcttg tcagtgggtt gtgttgtcat 5400
ctgtggcagg ataaccttga ctggcaggcc tgctgttgtc cctgatcgtg agatcttgta 5460
ccagcaattt gacgagatgg aagagtgctc taggcacttc ccctacctcg ccgagggcca 5520
gcagatcgcc gagcaattca gacaaaaggt gttgggactc cttcaagcga gcgccaagca 5580
ggcagaagaa ctgaagcctg ctgtccattc cgcgtggcct agggtggagg agttttggag 5640
gaagcacatg tggaactttg tcagcgggat ccagtatttg gcgggtttat ccaccctgcc 5700
cggcaaccca gccgtggcat caatgatgtc atttacagcg tcgctgacca gtcctctgag 5760
gacttctcag accctgctcc tcaacatact cggcggctgg atagccaccc aagtggctcc 5820
cccccccgcg tccacagctt ttgtcgtgag cggtctagca ggagccacgg ttggaagcat 5880
cgggctcggg agggtgttgg ttgatgtgct tgccggatac ggggccggtg tgtcgggtgc 5940
tctagtcgct ttcaagatca tgagcggcga gtgcccaacc accgaagaca tggtcaatct 6000
gctacccgcg ctgttgtcgc caggggctct cgtggtggga gtcgtgtgtg ctgccatctt 6060
aagacgccac gtaggccctg cggagggtgc caatcagtgg atgaacaggc taatagcctt 6120
tgcatcaaga ggcaaccatg tgtccccgac gcactacgtg cctgagactg acgcgtcaaa 6180
aaatgtgact cagatactca cttctcttac catcaccagc ctactccgca ggttacatca 6240
gtgggtcaat gaggacacgg ccaccccttg cgctacctca tggttacgcg acgtgtggga 6300
ctgggtgtgc acagtgctat ctgactttaa agtatggttg caagccaaac ttttccctcg 6360
cctgccgggg atccccttcc tctcgtgcca aacgggatat aggggagtct gggcagggga 6420
cggggtgtgc cacaccactt gtacctgtgg ggccgtgata gctggacacg tcaaaaatgg 6480
ctccatgaaa atcacaggac ccaagacatg cagcaacact tggcacggga ctttcccaat 6540
caacgccacc actaccggcc ccagcacacc acgaccagcc ctcaactatc agcgcgctct 6600
ttggcgggtg tccgccgagg actacgttga ggtgcggagg ttgggcgact gccactacgt 6660
ggtaggggtc actgctgacg ggttgaaatg cccttgccag gtacctgcac ctgaattctt 6720
cactgaggtc gatggcgtga ggatacaccg ttacgcgcca ccttgcaagc ccttgctcag 6780
ggacgaagtg actttctctg tgggtctttc aaactatgtc atagggtctc agctcccttg 6840
cgagccagag cctgacgtga ccgtggtcac ctcaatgctc acagaccccg tgcacatcac 6900
cgcagagacg gcagcacggc ggttgaagag ggggtccccc ccctctttag ccagctcatc 6960
ggccagccag ctgtctgcac cgtccctcaa ggctacttgc acaacctcca gagaccaccc 7020
ggacatggag ctcatcgagg ccaacctcct ctggaggcag gagatgggag gcaacatcac 7080
tcgagtcgag tcagagaaca aagtcgtagt ccttgactcc tttgagcctc taaccgctga 7140
gtatgacgag agggaaatct cagtgtcagc tgagtgccac aagccaccca ggcccaaatt 7200
ccctccagct cttccagtat gggccaggcc cgactacaat ccacctctcc tacaagcatg 7260
gcaaatgccc ggatacgagc ctccagtcgt gtctggatgt gccgtcgctc cacctaaacc 7320
ggcaccaatt cctccgccga ggcggaagag gctagtgcac ttggatgagt ccacggtctc 7380
gcacgccttg gcgcagcttg ccgacaaggt atgtgtggag agtggtagtg acccgggacc 7440
tagttcagac tcgggactat caatagccag tcctgtcccg cctgtctcaa caacaccgga 7500
tgccgcctgc tcaggagcag agtcctatag ctcaatgccc cctcttgagg gagagcctgg 7560
tgaccctgac ctgagctcag gctcttggtc cactgtgagc gatcagggtg atgtcgtgtg 7620
ttgttccatg tcctattcct ggacgggggc tctaataaca ccatgcgctg cggaggagga 7680
gaagcttcca ataaatccta tgagcaactc ccttataaga caccataaca tggtgtattc 7740
caccacatca cgcagcgcca gcctccgcca gaagaaggtc acatttgaca gactgcaagt 7800
gtttgaccaa cactaccagg atgtactaaa ggagattaag cttcgagcgt ccacggtgca 7860
ggcaaaactc ctatccatag aggaagcctg tgaccttacg ccatcgcact cagcacggtc 7920
caagtatgga tatggtgcgc aggacgttag aagccatgct agcaaggccg ttgaccacat 7980
ccgctccgtg tgggaggact tgctagaaga ctctgatacc ccaattccca cgaccatcat 8040
ggctaagaat gaagtcttct gcgtagatcc gtcgaagggt ggacgcaagc cggcacgcct 8100
aatagttttc ccagacttga gcgtgcgggt ctgcgagaag atggccctat acgacgtaac 8160
gcggaagttg ccacaggccg tgatgggttc agcatacgga ttccagtact cccctaacca 8220
gagggttgag tacttgctca aaatgtggcg gtcaaagaaa gtgcccatgg gcttttctta 8280
cgacaccagg tgttttgatt caaccgtcac tgagcgggat atccggactg agaacgaaat 8340
ctatcagtct tgccagctgg atcccgtggc aaggagggca gtatcatccc taacggagcg 8400
gctctacgta ggcggcccca tggtaaactc caagggacag ccatgtggct accgtagatg 8460
ccgcgccagt ggggtgctgc ccacgagcat gggaaacacc ctcacatgct atctgaaggc 8520
acaggccgcc tgcagggcgg ccaacatcaa agactatgac atgttggtgt gcggagatga 8580
cttagtggtc atttgtgaga gtgctggcgt ccaggaggac actgcgtcac tgcgagcatt 8640
cacggatgct atgaccaggt attcagctcc ccctggagac gccccgcaac ctacatatga 8700
ccttgagctc ataacatcat gctcatccaa cgtctccgtc gcccatgacg gcaacgggaa 8760
gagatattac tacctcacac gtgactgcac tactccactt gcgcgggctg catgggagac 8820
agcccgccac actccagtca attcgtggtt gggcaacatc attatgtttg cccccacgat 8880
atgggtgcgt atggttctga tgacccattt tttctccata ctccagtcgc aagaacaatt 8940
ggagaaagca ctcgactttg acatctacgg agtgacctac tccgtctctc cacttgatct 9000
cccagcaatc attcaacgac tccatggcat ggcagcattt tcactccacg gatattctcc 9060
agttgagctc aatagggtag gggcttgcct caggaaactt ggggtacctc ccttgagagc 9120
ctggagacat cgagccagag ctgtcagagc caaactcatt gtccaggggg ggaaagcggc 9180
tatatgcggt aagtacctct ttaactgggc ggtgaagacc aaattaaaac tcactccact 9240
ggtctccgcg agcaagcttg acttatcagg ctggttcgtg gcaggctacg acgggggggg 9300
catttttcac agcgtgtccc aggctcgacc ccgtctctta ctccttggcc tactcctact 9360
ctcagtaggg gtaggcatct ttttgctccc cgctcggtag agcggcacac actaggtaca 9420
ctccatagct aactgttcct tttttttttt tttttttttt tttttttttt tttttttttt 9480
ttcttttttt tttttttccc tctttcttcc cttctcatct tattctactt tctttcttgg 9540
tggctccatc ttagccctag tcacggctag ctgtgaaagg tccgtgagcc gcatgactgc 9600
agagagtgcc gtaactggtc tctctgcaga tcatgt 9636
<210> 12
<211> 9641
<212> DNA
<213> 丙型肝炎病毒CH6aFL_26m (CH6acc)(Hepatitis C virus)
<400> 12
gccagcccct taacggggcg acactccgcc attatcactc ccctgtgagg aactactgtc 60
ttcacgcaga aagcgtctag ccatggcgtt agtatgagtg tcgtgcagcc tccaggcccc 120
cccctcccgg gagagccata gtggtctgcg gaaccggtga gtacaccgga attgccagga 180
tgaccgggtc ctttccattg gatcaaaccc gctcaatgcc tggagatttg ggcgtgcccc 240
cgcaagactg ctagccgagt agcgttgggt tgcgaaaggc cttgtggtac tgcctgatag 300
ggtgcttgcg agtgccccgg gaggtctcgt agaccgtgca tcatgagcac acttccaaaa 360
ccccaaagaa aaaccaaaag gaacaccaac cgtcgcccaa tggacgtcaa gttcccgggt 420
ggcggtcaga tcgttggcgg agtttacttg ttgccgcgca ggggcccccg gttgggtgtg 480
cgcgcgacga ggaagacttc cgagcgatcc cagcccagag gcaggcgcca acctatacca 540
aaggcgcgcc agccccaggg caggcactgg gctcagcccg gatacccttg gcccctttat 600
ggaaacgagg gctgcgggtg ggcaggttgg ctcctgtccc cccgcggctc ccggccgcac 660
tggggcccca acgacccccg gcgtcgatcc cgcaatctgg gtaaggtcat cgatacccta 720
acgtgtgggt tcgccgatct catggggtac atccccgtcg tgggcgcgcc tttgggcggc 780
gtcgcggctg cgctcgcaca cggtgtgagg gcaatcgagg acgggatcaa ttatgcaaca 840
ggaaatcttc ccggttgctc tttctctatc ttcctcttgg cactactctc gtgcctaaca 900
acgccagctt cggctcttac ctacggcaac tccagtgacc tataccatct cacaaatgat 960
tgccccaact ccagcatcgt gctggaggcg gatgccatga tcttgcattt gcctggatgc 1020
ttgccttgtg taaaggtcga taataagtcc acctgctggc atgctgtgtc ccccacccta 1080
gccataccaa atgcttccac gcccgcaacg gggttccgta ggcacgtgga tcttcttgcg 1140
ggcgctgcgg tggtctgttc atctctgtat atcggggatt tgtgcggttc tctcttccta 1200
gcgggacaac tattcacctt ccagcctcgc cgtcactgga ctgtgcaaga ctgcaactgt 1260
tccatctata caggccacgt cactggccac aggatggctt gggacatgat gatgaactgg 1320
tcacccacaa ccactctggt cctatctagc atcttgaggg tacctgagat ctgtgcgagt 1380
gtgatatctg gtggccactg ggggatgcta ctagccgttg cctactttgg tatggctggt 1440
aactggctaa aagtcctggc tgttctgttc ttatttgcag gggtcgaagc aaccaccacc 1500
accggctccg cgataagcaa agctacttcc ggctttaccg cgcttttcaa ccccggtgcc 1560
aggcagaatc tacacctcac caactccagt ggtagctggc atataaacag gactgctttg 1620
aactgcaatg attccctcca tacggggttc atagcgtcac tcttttacgc ccacaagttc 1680
aactcctcgg gctgcccaga gcggatggcc gcatgtaagc cccttgctga cttcaagcag 1740
gggtggggcc aaataaccta caaagtcaac atctcaggcc cctctgacga ccgtccctac 1800
tgttggcatt acgctcccag gccatgtgac gtggtggcag cccgcacggt gtgcggccct 1860
gtttactgct tcacgcctag ccctgttgtg gtaggaacca ctgacaggcg gggcaacccc 1920
acatacacct ggggggagaa tgagacggat gtgttcatgc tggaaagcct tcggccccct 1980
actggggggt ggtttgggtg cacctggatg aacgctacgg gctttaccaa gacctgtggt 2040
gccccgccat gtcagatagt cccaggggat tacaacagct ctgccaatga gcttttgtgc 2100
cccaccgatt gcttccgtaa acatccggga gccacatacc agcggtgtgg atcaggaccc 2160
tggttaacac ctaggtgtct agtggactat ccctacagac tgtggcacta cccctgcact 2220
gtaaacttca ccttgcataa ggtcaggatg tttgtggggg gcgttgagca ccggtttgac 2280
gccgcatgta attggaccag gggcgagcgg tgcgagttac atgacagaga caggattgaa 2340
atgagcccgc tgcttttctc aaccacgcag cttgccatac ttccatgttc attttccacc 2400
atgccggcct tgtcaactgg cctcattcac ctgcatcaga acatagtgga cgtgcagtac 2460
ctctacggag tctcctcgag cgtcacctcg tgggtagtaa agtgggagta cattgtcctg 2520
atgtttctgg ttctggcaga tgctcggatt tgtacgtgtc tctggttaat gctgctcata 2580
agtagggttg aggcagcagt ggagaggctt gtcgtcctca acgcggctag cgccgccggc 2640
actgctggct ggtggtgggc ggtgcttttc ctgtgctgtg cttggtacgt gaaaggtcgc 2700
cttgtgcctg cgtgtaccta catggcgctg ggaatgtggc cattgctctt agcaatcttg 2760
gccctgcctc agcgagcgta tgccatggac aatgagcaag cggcatccct cggagctgtt 2820
ggtctattgg ttgtcaccac cttcaccatc acccccatgt acaagaagct attgacctgc 2880
ttcatttggt ggaatcagta tttcctcgcc cgagctgagg ccatggtaca cgagtgggtg 2940
cccgacctac gggttagggg tggtagggat tccatcatct tatttacctg cttgttccat 3000
ccacagctgg ggtttgaggt cactaagatc ctactagcta tcctggcccc tctatacatc 3060
ttgcagtaca gtttgctcaa agtgccttac tttgtgcgcg cccacatact cctgcgtgct 3120
tgcctgcttg ttcgtgggct ggcagggggt aagtacgtgc aggcgtgcct tctgaggttg 3180
ggcgcttgga ctggtaccta tgtttatgac catctcgccc ccctttctgg ctgggctagc 3240
gacggactgc gcgatctagc agttgcagtc gagccggtca ttttctctcc tatggagaag 3300
aaaattatca cctggggtgc ggacactgcc gcgtgtggtg acatcttgag tggtctcccg 3360
gtgtcagcga ggttggggaa tttggtgcta ctaggacccg cggatgatat gcggcgcgga 3420
ggttggaagc ttttggctcc catcactgcg tatgcacagc agacgagggg cctagtcggc 3480
accattgtga ccagcctaac cggacgtgac aaaaatgagg tcgaagggga ggtacaggtg 3540
gtctccacgg ctacccaatc cttcctggcg acctccatta acggtgtcat gtggaccgtt 3600
tatcatgggg ccggttcaaa gactctcgcc ggaccgaaag gaccagtgtg tcaaatgtac 3660
accaacgtgg acaaggacct agtagggtgg ccatctccca agggagcaag atccctcacc 3720
ccatgtactt gtggctctag tgacctctat ttggtcacga gggaggccga cgtcatcccc 3780
gcaagacgca ggggtgacag ccgtgctgcc ctcctctctc ctaggcccat aagcaccttg 3840
aaaggctctt cgggaggccc cattatgtgt ccctcggggc acgttgtggg actcttccga 3900
gcagccgtat gcacaagggg tgtagcaaaa tccttagatt ttatcccagt ggaaaacatg 3960
gagacgacta tgcgctctcc ctcattcaca gacaattcca caccccctgc ggtgccccag 4020
acctatcagg tggggtacct gcacgcacca acaggcagcg gaaagagcac ccgtgtcccg 4080
gcggcgtacg ctagccaggg ctacaaggtg ttggtcttga acccatcggt ggcggcgacg 4140
cttagctttg gctcgtacat gaggcaagct tacggcgtgg agccgaatgt ccgggctggg 4200
gtcaggacta taactacagg gggcgccatc acatactcca catatgggag attcttggcc 4260
gatgggggat gttccggagg ggcgtatgac atcatcatct gcgatgagtg ccactctaca 4320
gaccctacga cggtgttggg cattggcacg gttctcgacc aggctgagac cgccggggtt 4380
cgtcttaccg tgcttgcaac agcaacgccg ccgggttctg tcacagtacc ccatcctaac 4440
ataacagaga tagccctccc aactacggga gaaataccat tttatggaaa gggcatccct 4500
cttgagtaca tcaaaggggg aagacacctc atattctgtc actcgaagaa gaagtgcgac 4560
gagctggccg ggaaactgaa gtcactcggc ttaaacgccg tcgcattcta cagaggtgtc 4620
gacgtgtccg ttatccccac ttcgggcgac gtcgtcgtct gcgcgacaga cgcccttatg 4680
accggctaca caggcgattt cgattccgtc atcgactgta acgtagccgt gacacaggtg 4740
gtggatctca gcttggaccc gacattttcc atagagacta ccaccgtccc tcaggatgcg 4800
gtatcacgga gccaacgacg aggccgcacg gggcggggta aaccgggggt gtacaggttt 4860
gtctcccagg gggagaggcc ttcgggtatg ttcgacaccg tcgtcctgtg tgaggcttat 4920
gacacgggat gcgcgtggta cgaactaacc ccttctgaaa caactgtcag gctgagggcc 4980
tatctgaaca ctcctggcct tcccttatgc caagaccacc tggaattttg ggaaggcgtg 5040
tttactggct tgacccacat agacgcccac tttctgtctc agacgaagca ggggggtgag 5100
aacttcgcgt acctcgtggc ataccaggct acagtatgcg ccagggccaa agcccccccg 5160
ccttcttggg atacgatgtg gaagtgtctc atcagactta aacccaccct taccggcccc 5220
actccacttt tgtatcggct gggggccgta caaaatgagg taataacaac ccaccccata 5280
acccaataca tcatgacctg catgtctgcg gatttggagg tcatcaccag cacgtgggtc 5340
cttgtgggtg gagtcctagc cgcgctcgca tcctactgct tgtcagtggg ttgtgttgtc 5400
atctgtggca ggataacctt gactggcagg cctgctgttg tccctgatcg tgagatcttg 5460
taccagcaat ttgacgagat ggaagagtgc tctaggcact tcccctacct cgccgagggc 5520
cagcagatcg ccgagcaatt cagacaaaag gtgttgggac tccttcaagc gagcgccaag 5580
caggcagaag aactgaagcc tgctgtccat tccgcgtggc ctagggtgga ggagttttgg 5640
aggaagcaca tgtggaactt tgtcagcggg atccagtatt tggcgggttt atccaccctg 5700
cccggcaacc cagccgtggc atcaatgatg tcatttacag cgtcgctgac cagtcctctg 5760
aggacttctc agaccctgct cctcaacata ctcggcggct ggatagccac ccaagtggct 5820
cccccccccg cgtccacagc ttttgtcgtg agcggtctag caggagccac ggttggaagc 5880
atcgggctcg ggagggtgtt ggttgatgtg cttgccggat acggggccgg tgtgtcgggt 5940
gctctagtcg ctttcaagat catgagcggc gagtgcccaa ccaccgaaga catggtcaat 6000
ctgctacccg cgctgttgtc gccaggggct ctcgtggtgg gagtcgtgtg tgctgccatc 6060
ttaagacgcc acgtaggccc tgcggagggt gccaatcagt ggatgaacag gctaatagcc 6120
tttgcatcaa gaggcaacca tgtgtccccg acgcactacg tgcctgagac tgacgcgtca 6180
aaaaatgtga ctcagatact cacttctctt accatcacca gcctactccg caggttacat 6240
cagtgggtca atgaggacac ggccacccct tgcgctacct catggttacg cgacgtgtgg 6300
gactgggtgt gcacagtgct atctgacttt aaagtatggt tgcaagccaa acttttccct 6360
cgcctgccgg ggatcccctt cctctcgtgc caaacgggat ataggggagt ctgggcaggg 6420
gacggggtgt gccacaccac ttgtacctgt ggggccgtga tagctggaca cgtcaaaaat 6480
ggctccatga aaatcacagg acccaagaca tgcagcaaca cttggcacgg gactttccca 6540
atcaacgcca ccactaccgg ccccagcaca ccacgaccag ccctcaacta tcagcgcgct 6600
ctttggcggg tgtccgccga ggactacgtt gaggtgcgga ggttgggcga ctgccactac 6660
gtggtagggg tcactgctga cgggttgaaa tgcccttgcc aggtacctgc acctgaattc 6720
ttcactgagg tcgatggcgt gaggatacac cgttacgcgc caccttgcaa gcccttgctc 6780
agggacgaag tgactttctc tgtgggtctt tcaaactatg tcatagggtc tcagctccct 6840
tgcgagccag agcctgacgt gaccgtggtc acctcaatgc tcacagaccc cgtgcacatc 6900
accgcagaga cggcagcacg gcggttgaag agggggtccc ccccctcttt agccagctca 6960
tcggccagcc agctgtctgc accgtccctc aaggctactt gcacaacctc cagagaccac 7020
ccggacatgg agctcatcga ggccaacctc ctctggaggc aggagatggg aggcaacatc 7080
actcgagtcg agtcagagaa caaagtcgta gtccttgact cctttgagcc tctaaccgct 7140
gagtatgacg agagggaaat ctcagtgtca gctgagtgcc acaagccacc caggcccaaa 7200
ttccctccag ctcttccagt atgggccagg cccgactaca atccacctct cctacaagca 7260
tggcaaatgc ccggatacga gcctccagtc gtgtctggat gtgccgtcgc tccacctaaa 7320
ccggcaccaa ttcctccgcc gaggcggaag aggctagtgc acttggatga gtccacggtc 7380
tcgcacgcct tggcgcagct tgccgacaag gtatgtgtgg agagtggtag tgacccggga 7440
cctagttcag actcgggact atcaatagcc agtcctgtcc cgcctgtctc aacaacaccg 7500
gatgccgcct gctcaggagc agagtcctat agctcaatgc cccctcttga gggagagcct 7560
ggtgaccctg acctgagctc aggctcttgg tccactgtga gcgatcaggg tgatgtcgtg 7620
tgttgttcca tgtcctattc ctggacgggg gctctaataa caccatgcgc tgcggaggag 7680
gagaagcttc caataaatcc tatgagcaac tcccttataa gacaccataa catggtgtat 7740
tccaccacat cacgcagcgc cagcctccgc cagaagaagg tcacatttga cagactgcaa 7800
gtgtttgacc aacactacca ggatgtacta aaggagatta agcttcgagc gtccacggtg 7860
caggcaaaac tcctatccat agaggaagcc tgtgacctta cgccatcgca ctcagcacgg 7920
tccaagtatg gatatggtgc gcaggacgtt agaagccatg ctagcaaggc cgttgaccac 7980
atccgctccg tgtgggagga cttgctagaa gactctgata ccccaattcc cacgaccatc 8040
atggctaaga atgaagtctt ctgcgtagat ccgtcgaagg gtggacgcaa gccggcacgc 8100
ctaatagttt tcccagactt gagcgtgcgg gtctgcgaga agatggccct atacgacgta 8160
acgcggaagt tgccacaggc cgtgatgggt tcagcatacg gattccagta ctcccctaac 8220
cagagggttg agtacttgct caaaatgtgg cggtcaaaga aagtgcccat gggcttttct 8280
tacgacacca ggtgttttga ttcaaccgtc actgagcggg atatccggac tgagaacgaa 8340
atctatcagt cttgccagct ggatcccgtg gcaaggaggg cagtatcatc cctaacggag 8400
cggctctacg taggcggccc catggtaaac tccaagggac agccatgtgg ctaccgtaga 8460
tgccgcgcca gtggggtgct gcccacgagc atgggaaaca ccctcacatg ctatctgaag 8520
gcacaggccg cctgcagggc ggccaacatc aaagactatg acatgttggt gtgcggagat 8580
gacttagtgg tcatttgtga gagtgctggc gtccaggagg acactgcgtc actgcgagca 8640
ttcacggatg ctatgaccag gtattcagct ccccctggag acgccccgca acctacatat 8700
gaccttgagc tcataacatc atgctcatcc aacgtctccg tcgcccatga cggcaacggg 8760
aagagatatt actacctcac acgtgactgc actactccac ttgcgcgggc tgcatgggag 8820
acagcccgcc acactccagt caattcgtgg ttgggcaaca tcattatgtt tgcccccacg 8880
atatgggtgc gtatggttct gatgacccat tttttctcca tactccagtc gcaagaacaa 8940
ttggagaaag cactcgactt tgacatctac ggagtgacct actccgtctc tccacttgat 9000
ctcccagcaa tcattcaacg actccatggc atggcagcat tttcactcca cggatattct 9060
ccagttgagc tcaatagggt aggggcttgc ctcaggaaac ttggggtacc tcccttgaga 9120
gcctggagac atcgagccag agctgtcaga gccaaactca ttgtccaggg ggggaaagcg 9180
gctatatgcg gtaagtacct ctttaactgg gcggtgaaga ccaaattaaa actcactcca 9240
ctggtctccg cgagcaagct tgacttatca ggctggttcg tggcaggcta cgacgggggg 9300
ggcatttttc acagcgtgtc ccaggctcga ccccgtctct tactccttgg cctactccta 9360
ctctcagtag gggtaggcat ctttttgctc cccgctcggt agacgagcgg ccctagcaac 9420
actccataat tccttttccc ttttttattt tttttctttt tttttttttt tttttttttt 9480
tttttttttt tttttttttt tttttaacct ttcttatctg tctatcttct aaacttcttt 9540
cttggtggct ccatcttagc cctagtcacg gctagctgtg aaaggtccgt gagccgcatg 9600
actgcagaga gtgctgatac tggcctctct gcagatcatg t 9641
Claims (11)
1.扩增获得HCV CH6a的核苷酸全长序列(附件，见序列CH6aFL-WT)。
2.由CH6a毒株为基础构建的C-NS2与JFH1嵌合(带有GFP)病毒质粒CH6aC-NS2/JFH1-GFP(基因结构为pJFH1_5’UTR/CH6a_C-NS2/JFH1_NS3-5A/EGFP/JFH1_NS5B-3UTR)。转染Huh7.5.1获得一系列适应性突变(见附表1和附图1)，用反向遗传技术证明感染第5代病毒(P5)的氨基酸突变促进了病毒的传播并增加了病毒的滴度(见附图1)。
3.CH6aC-NS2/JFH1-GFP感染第9代(P9)获得的新突变---测序证明了P5突变依然存在，而且增加了新的突变(见附表1)。
4.由该毒株的Core-NS5A与JFH1嵌合的病毒质粒pJFH1_5’UTR/CH6a_C-5A/I356M(A1405G)/N587S(A1587G)/I831V(A2831G)/L881F(C2981T)/K1303R(A4248G)/A1470S(T4725C)/V1556L(G5003T)/A1678S(G5368T/K1696R(A5429G)/I1721F(A5498T)/L1796M(T5723A)/JFH1_5B-3UTR带有11个突变(11m,包含来自Core-NS2 P5的4个突变点)转染Huh7.5.1可以复制，获得病毒；Core-NS5A重新病毒包括带7m(低水平复制，但未得到病毒)。
5.CH6aC-5A_11m/JFH1(基因结构pCH6a_C-5A/JFH1_5B-3UTR带11个突变)连续传代感染后，获得新适应性突变，构建CH6aC-5A_15m/JFH1和CH6aC-5A_18m/JFH1(见附表2和附图2)。
6.将CH6aC-5A_18m/JFH1中的突变加上I833T/R932G/D2987G/Y2989F构建CH6aORF_22m，该病毒低水平但逐渐传播，获得新突变(见附表3)，之后构建了CH6aORF_24m，CH6aORF_25m，CH6aORF_26m和CH6aFL_26m(CH6acc)。(见附表3和附图3)。
7.这些CH6a全长病毒对HCV药物呈剂量依赖性反应(见附图4)。
8.本专利权利要求以上提到的全部基因序列以及鉴定的突变，以及用这些突变进行其它毒株的培养，建立HCV细胞感染模型。
9.本专利权利要求以上提到的全部基因序列以及鉴定的突变，构建的各种重组病毒，以及用这些突变进行其它毒株的培养，建立HCV细胞感染模型。
10.本专利权利要求以上提到的全部基因序列以及鉴定的突变，构建的各种重组病毒，进行疫苗和药物研发和生产。
11.本专利权利要求以上提到的各种重组病毒进行丙肝治疗的药物靶点发现和治疗。
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| CN113881643A (zh) * | 2021-10-08 | 2022-01-04 | 中山大学 | 一种6a型丙肝病毒亚基因组复制子及应用 |
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