CN110124883A - Online sterilizing centrifugation systems and method - Google Patents
Online sterilizing centrifugation systems and method Download PDFInfo
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- CN110124883A CN110124883A CN201910498563.0A CN201910498563A CN110124883A CN 110124883 A CN110124883 A CN 110124883A CN 201910498563 A CN201910498563 A CN 201910498563A CN 110124883 A CN110124883 A CN 110124883A
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- Prior art keywords
- centrifuge
- sample
- gas
- online
- sterilizing
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/06—Other accessories for centrifuges for cleaning bowls, filters, sieves, inserts, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
- A61L2/06—Hot gas
- A61L2/07—Steam
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/02—Other accessories for centrifuges for cooling, heating, or heat insulating
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- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Centrifugal Separators (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of online sterilizing centrifugation systems and methods.The online sterilizing centrifugation systems include sample storage tank, centrifuge, steam generator and gas reservoir；The outlet of the sample storage tank is connected by sample delivery pipeline with the centrifuge, is formed sample and is centrifuged main road；The steam generator is connected by steam conveying pipeline with the outlet of the sample storage tank, is formed steam and is conveyed branch；The steam generator sterilizes to the sample delivery pipeline and the centrifuge for generating steam；The gas reservoir is connected by gas transfer pipeline with the outlet of the sample storage tank, is formed gas and is conveyed branch；The gas reservoir to the sample delivery pipeline and the centrifuge for ventilating after sterilization.The system is able to achieve online sterilizing, easy to operate, effectively reduces the microbiological contamination risk to Centrifuge A sample.
Description
Technical field
The present invention relates to a kind of online sterilizing centrifugation systems and methods.
Background technique
Tube centrifuge can not sterilize mostly in the prior art, when the life more demanding to aseptic using centrifuge
When Tetramune is centrifuged, current common centrifuge is it is difficult to ensure that there is very big dye without miscellaneous bacteria in the biological products after centrifugation
Bacterium risk.
When carrying out the centrifugation of fermentation liquid using tube centrifuge, for a long time by the puzzlement of centrifugal process microbiological contamination problem, interference is real
Test progress and test result.
Summary of the invention
For the defect that centrifuge in the prior art can not sterilize, a kind of online centrifugation systems and side of sterilizing is provided
Method, which is able to achieve online sterilizing, easy to operate, effectively reduces the microbiological contamination risk to Centrifuge A sample.
The present invention provides a kind of online sterilizing centrifugation systems comprising sample storage tank, centrifuge, steam generator are gentle
Body storage tank；
The outlet of the sample storage tank is connected by sample delivery pipeline with the centrifuge, and sample centrifugation master is formed
Road；
The steam generator is connected by steam conveying pipeline with the outlet of the sample storage tank, and steam conveying is formed
Branch；The steam generator sterilizes to the sample delivery pipeline and the centrifuge for generating steam；
The gas reservoir is connected by gas transfer pipeline with the outlet of the sample storage tank, and gas conveying branch is formed
Road；The gas reservoir to the sample delivery pipeline and the centrifuge for ventilating after sterilization.
The steam that the present invention is generated using steam generator sterilizes to sample delivery pipeline and centrifuge, further according to
The demand of Centrifuge A sample is passed through corresponding gas to system, so that system is kept positive pressure, to reduce to be centrifuged in Centrifuge A sample
The microbiological contamination risk of process.
The sample storage tank is for storing to Centrifuge A sample.
Preferably, the steam generator is also connected with the outlet of the gas reservoir, it is further defeated to the gas
Pipeline is sent to sterilize.
The centrifuge by described to Centrifuge A sample for being centrifuged.Preferably, the centrifuge is tube centrifuge；
More preferably, the centrifuge is clarification type tube centrifuge (i.e. GQ series of tubular centrifuge), such as GQ-76 type tubular type is centrifuged
Machine.
Preferably, the centrifuge is additionally provided with temperature sensor.
Preferably, the centrifuge is additionally provided with cooling system.
Preferably, the outlet of the gas reservoir is additionally provided with air fine filter.
The present invention also provides a kind of online sterilizing centrifugal methods, and above-mentioned online sterilizing centrifugation systems is used to carry out,
The following steps are included:
(1) steam generated using the steam generator goes out to the sample delivery pipeline and the centrifuge
Bacterium；
(2) it is ventilated using the gas reservoir to the sample delivery pipeline and the centrifuge；
(3) it is carried out to Centrifuge A sample by the sample delivery Pipeline transport to the centrifuge in the sample storage tank
Centrifugation.
Preferably, the gas stored in the gas reservoir can be selected according to the demand to Centrifuge A sample, Ke Yiwei
The nitrogen and carbon dioxide mix gas, pure nitrogen gas or filtrated air of arbitrary proportion.Into system, on the one hand ventilation keeps system
On the other hand positive pressure can select different gas according to the demand to Centrifuge A sample, reduce subsequent centrifugal process and treat centrifugation
The influence of sample.For example, storing nitrogen in the gas reservoir when Centrifuge A sample is anaerobic bacteria or facultative anaerobic bacteria when described
With carbon dioxide mix gas or high pure nitrogen, wherein the ratio both in nitrogen and carbon dioxide mix gas can be according to wait be centrifuged
The demand of sample is selected；When described when Centrifuge A sample is aerobic bacteria, filtrated air is stored in the gas reservoir.
Preferably, when being sterilized, the temperature in the centrifuge is 105 DEG C~121 DEG C, sterilization time in step (1)
For 20min or more, sterilization time is preferably 20~120min.
Preferably, the time of the ventilation is 40min or more in step (2), per hour in the interior sample delivery pipeline
The gas volume flowed through is (0.15~0.4) × V, and V is the volume of rotary drum in the centrifuge.
More preferably, in step (2), the time of the ventilation is 40~120min.
Preferably, in step (3), again to described to Centrifuge A sample after the temperature of the centrifuge is down to 4 DEG C~37 DEG C
It is centrifuged.
Without prejudice to the field on the basis of common sense, above-mentioned each optimum condition, can any combination to get the present invention it is each preferably
Example.
The reagents and materials used in the present invention are commercially available.
The positive effect of the present invention is that: steam generator and gas reservoir are connected by pipeline, make system of the present invention
System realizes the function of sterilizing online, easy to operate, ensure that the aseptic of system, dramatically reduces to Centrifuge A sample
Microbiological contamination risk.It reduces centrifugal process while so that system is kept positive pressure to system venting after sterilizing terminates and treats centrifugation
The influence of sample.
Detailed description of the invention
Fig. 1 is the schematic diagram of the online sterilizing centrifugation systems of the embodiment of the present invention 1；
Fig. 2 is the schematic diagram of the online sterilizing centrifugation systems of the embodiment of the present invention 2；
Fig. 3 is the temperature variation of sterilization process rotary drum in the online sterilizing centrifugal method of the embodiment of the present invention 3；
Fig. 4 is the temperature of sterilization process rotary drum when sterilization time is 10min in the online sterilizing centrifugal method of the embodiment of the present invention 4
Spend variation diagram；
Fig. 5 is the temperature of sterilization process rotary drum when sterilization time is 30min in the online sterilizing centrifugal method of the embodiment of the present invention 4
Spend variation diagram.
Description of symbols:
1- sample storage tank, 2- centrifuge, 3- steam generator, 4- gas reservoir, 5- air fine filter；
101- sample delivery pipeline, 102- steam conveying pipeline, 103- gas transfer pipeline.
Specific embodiment
The present invention is further illustrated below by the mode of embodiment, but does not therefore limit the present invention to the reality
It applies among a range.In the following examples, the experimental methods for specific conditions are not specified, according to conventional methods and conditions, or according to quotient
The selection of product specification.
Embodiment 1
As shown in Figure 1, present embodiments providing a kind of online sterilizing centrifugation systems comprising: sample storage tank 1, centrifuge
2, steam generator 3 and gas reservoir 4,
The outlet of sample storage tank 1 is connected by sample delivery pipeline 101 with centrifuge 2, is formed sample and is centrifuged main road；Its
In, sample storage tank 1 is for storing to Centrifuge A sample；Centrifuge 2 to Centrifuge A sample for that will be centrifuged；
Steam generator 3 is connected by steam conveying pipeline 102 with the outlet of sample storage tank 1, and steam conveying branch is formed
Road；Steam generator 3 sterilizes to sample delivery pipeline 101 and centrifuge 2 for generating steam；
Gas reservoir 4 is connected by gas transfer pipeline 103 with the outlet of sample storage tank 1, is formed gas and is conveyed branch；
Gas reservoir 4 to sample delivery pipeline 101 and centrifuge 2 for ventilating after sterilization.
Wherein, centrifuge 2 is GQ-76 type tube centrifuge, and rotary drum volume is 2L, and inside is equipped with temperature sensor, also sets
There is cooling system.
The outlet of gas reservoir 1 is additionally provided with air fine filter 5, for ensuring that gas reaches sterile after filtering.
Embodiment 2
As shown in Fig. 2, compared with Example 1, in online sterilizing centrifugation systems provided in this embodiment, difference is, steam
Vapour generator 3 is also connected by steam conveying pipeline 102 with the outlet of gas reservoir 4, further to gas transfer pipeline 103
It sterilizes.
Embodiment 3
A kind of online sterilizing centrifugal method, the online sterilizing centrifugation systems provided using embodiment 2 are be provided
It carries out comprising following steps:
(1) preparation
2 bottom connecting tube feed inlet of centrifuge and 1 discharge port of sample storage tank and the gas equipped with air fine filter 5
Transfer pipeline 103 is connected with the leather hose with four way valve.In the present embodiment, centrifuge 2 is GQ-76 type tube centrifuge, is turned
Drum volume is 2L, and inside is equipped with temperature sensor, is additionally provided with cooling system.
(2) it sterilizes
The steam generated using steam generator 3 sterilizes to sample delivery pipeline 101 and centrifuge 2, concrete operations
Include:
Reach simultaneously connection with gas transfer pipeline 103 step 1: adjusting four way valve and it being made to constitute 1 discharge port of sample storage tank
To the access of 1 bottom connecting tube feed inlet of centrifuge；Step 2: closing the air intake valve on gas transfer pipeline 103, sample is opened
The steam valve that 1 discharge port of product storage tank and gas transfer pipeline 103 have imports steam into access and sterilizes；Step 3:
Tube centrifuge power supply is opened, rotary drum temperature is observed, rises to 105 DEG C of beginning timing, timing 20min to it；Step 4: sterilizing knot
The steam valve that 1 discharge port of sample storage tank and gas transfer pipeline 103 have, temperature such as Fig. 3 in the process rotary drum are closed after beam
It is shown.
(3) it divulges information
It is ventilated using gas reservoir 4 to sample delivery pipeline 101 and centrifuge 2, concrete operations include:
Air inlet valve on the front opening gas transfer pipeline 103 of feed liquid centrifugation is to access ventilation Continuous aeration
40min, ventilation volume flow are 0.3m3/ h makes it keep positive pressure.The sky on gas transfer pipeline 103 is closed after ventilation
Gas air intake valve.
(4) it is centrifuged
Being delivered to centrifuge 2 by sample delivery pipeline 101 to Centrifuge A sample and be centrifuged in sample storage tank 1, specifically
Operation includes:
Step 1: opening the refrigerating cycle pump of centrifuge 2, cools down to rotary drum, its temperature is made to be down to 37 DEG C.Second
Step: the motor of starting centrifuge 2, to stabilization of speed to setting value.Step 3: the discharge port of unscrewing sample storage tank 1, is pressed
0.05m3/ h is fed to centrifuge 2, and the liquid outlet of centrifuge 2 collects supernatant with bucket.It should be noted that feed liquid in second step
Volume flow can be selected according to the property of feed liquid itself and the treating capacity of centrifuge, refer specifically to per hour in sample it is defeated
The volume for sending the feed liquid flowed through in pipeline 101 is (0.005~0.05) × V, and V is the volume of rotary drum in the centrifuge.Feed liquid
Volume flow only influence perceptions and the texture such as density, viscosity of bacterium mud after centrifugation, the influence very little to microbiological contamination.
Using the present embodiment online sterilizing centrifugal method respectively to blank cultures and bifidus bacillus fermented liquid carry out from
The heart randomly selects the sample after being centrifuged and carries out Bacteria Detection, and detection method is as follows:
Test sample source: the sample after randomly selecting centrifugation is as test sample
Nutrient agar configuration:
It weighs in 17.1g nutrient agar powder and beaker, adds ultrapure water 1000mL, dissolve by heating, packing to three
In the bottle of angle, 121 DEG C sterilize 20 minutes, are cooled to 50 DEG C or so, down in culture dish, cooled and solidified is spare.
Preparation of samples:
Test sample 1g is weighed, is added in 0.9% aseptic sodium chloride solution of 9ml, is mixed, is done 10 times and be serially diluted, it is suitable to take
Dilution test solution 0.1ml is added on the agar medium got ready, is smoothened with glass rod, 3 parts of a formula, is inverted, constant temperature training
It supports and is cultivated 48 hours in case, observation daily is as a result, the clump count grown on record culture dish.
The results show that the sample for the centrifugal method processing that sterilized online using the present embodiment is without bacterial growth.
Embodiment 4
The purpose of the present embodiment is that investigating the influence of sterilization time.
Compared with Example 3, in online sterilizing centrifugal method provided in this embodiment, difference is, the third of step (2)
Walk as follows: opening tube centrifuge power supply, observe rotary drum temperature, rise to 105 DEG C of beginnings timing to it, difference timing 10min with
30min.Remaining operation is same as Example 3.Temperature difference is as shown in Figures 4 and 5 in rotary drum in step (2).
The detection method provided using embodiment 3 carries out Bacteria Detection, testing result such as 1 institute of table to the sample after centrifugation
Show.
Table 1
| Serial number | Sterilization time (min) | Test result |
| 1 | 10 | 3CFU/g (has miscellaneous bacteria) |
| 2 | 30 | 0 (no miscellaneous bacteria) |
1 test result of table refers to the clump count turned out in every gram of test sample.
Embodiment 5
The purpose of the present embodiment is that investigating the influence of ventilation process gas flow rate.
Compared with Example 3, in online sterilizing centrifugal method provided in this embodiment, difference is, in step (3), gas
The volume flow of gas is respectively 0.2m in body transfer pipeline 1033/ h and 0.4m3/h.Remaining operation is same as Example 3.
The detection method provided using embodiment 3 carries out Bacteria Detection, testing result such as 2 institute of table to the sample after centrifugation
Show.
Table 2
| Serial number | Volume flow (the m of gas in sample delivery pipeline3/h) | Test result |
| 1 | 0.2 | 5CFU/g (has miscellaneous bacteria) |
| 2 | 0.4 | Without miscellaneous bacteria |
2 test result of table refers to the clump count turned out in every gram of test sample.
Comparative example 1
This comparative example provides a kind of online centrifugal method, compared with the online sterilizing centrifugal method of embodiment 3, lacks and goes out
Bacterium and aeration step, specifically includes the following steps:
Being delivered to centrifuge 2 by sample delivery pipeline 101 to Centrifuge A sample and be centrifuged in sample storage tank 1, specifically
Operation includes:
Step 1: opening the refrigerating cycle pump of centrifuge 2, cools down to rotary drum, make 30 DEG C of its temperature.Step 2:
The motor for starting centrifuge 2, to stabilization of speed to setting value.Step 3: the discharge port of unscrewing sample storage tank 1, by 0.05m3/h
It is fed to centrifuge 2, the liquid outlet of centrifuge 2 collects supernatant with bucket.
Blank cultures and bifidus bacillus fermented liquid are centrifuged respectively using the online centrifugal method of the present embodiment, with
Machine extracts the sample after centrifugation and carries out Bacteria Detection using the detection method that embodiment 3 provides, the results show that using this implementation
The sample of the online centrifugal method processing of example has varied bacteria growing, specially 2.6*102CFU/g。
Claims (10)
1. a kind of online sterilizing centrifugation systems, which is characterized in that the online sterilizing centrifugation systems include sample storage tank, centrifugation
Machine, steam generator and gas reservoir；
The outlet of the sample storage tank is connected by sample delivery pipeline with the centrifuge, is formed sample and is centrifuged main road；
The steam generator is connected by steam conveying pipeline with the outlet of the sample storage tank, and steam conveying branch is formed
Road；The steam generator sterilizes to the sample delivery pipeline and the centrifuge for generating steam；
The gas reservoir is connected by gas transfer pipeline with the outlet of the sample storage tank, is formed gas and is conveyed branch；
The gas reservoir to the sample delivery pipeline and the centrifuge for ventilating after sterilization.
2. sterilizing centrifugation systems as described in claim 1 online, which is characterized in that the steam generator also with the gas
The outlet of storage tank is connected, for sterilizing to the gas transfer pipeline.
3. online sterilizing centrifugation systems as described in claim 1, which is characterized in that the centrifuge is tube centrifuge.
4. online sterilizing centrifugation systems as described in claim 1, which is characterized in that the centrifuge is additionally provided with temperature sensing
Device.
5. online sterilizing centrifugation systems as described in claim 1, which is characterized in that the centrifuge is additionally provided with cooling system.
6. online sterilizing centrifugation systems as described in claim 1, which is characterized in that the outlet of the gas reservoir is additionally provided with
Air fine filter.
7. a kind of online sterilizing centrifugal method, which is characterized in that it uses the described in any item online sterilizings of claim 1~6
Centrifugation systems carry out, the online sterilizing centrifugal method the following steps are included:
(1) steam generated using the steam generator sterilizes to the sample delivery pipeline and the centrifuge；
(2) it is ventilated using the gas reservoir to the sample delivery pipeline and the centrifuge；
(3) in the sample storage tank to Centrifuge A sample by the sample delivery Pipeline transport to the centrifuge carry out from
The heart.
8. online sterilizing centrifugal method as claimed in claim 7, which is characterized in that the gas of storage is in the gas reservoir
Nitrogen and carbon dioxide mix gas, pure nitrogen gas or filtrated air；
And/or in step (3), after the temperature of the centrifuge is down to 4 DEG C~37 DEG C again to it is described to Centrifuge A sample carry out from
The heart.
9. online sterilizing centrifugal method as claimed in claim 7, which is characterized in that described when being sterilized in step (1)
Temperature in centrifuge is 105 DEG C~121 DEG C, and sterilization time is 20min or more.
10. online sterilizing centrifugal method as claimed in claim 7, which is characterized in that in step (2), the time of the ventilation
For 40min or more, the gas volume by flowing through in the sample delivery pipeline interior per hour is (0.15~0.4) × V, and V is
The volume of rotary drum in the centrifuge.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910498563.0A CN110124883A (en) | 2019-06-10 | 2019-06-10 | Online sterilizing centrifugation systems and method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910498563.0A CN110124883A (en) | 2019-06-10 | 2019-06-10 | Online sterilizing centrifugation systems and method |
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| Publication Number | Publication Date |
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| CN110124883A true CN110124883A (en) | 2019-08-16 |
Family
ID=67581023
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060009341A1 (en) * | 2004-07-08 | 2006-01-12 | Yoshinori Tobita | Centrifugal separator |
| CN202830031U (en) * | 2012-09-29 | 2013-03-27 | 北京华都诗华生物制品有限公司 | Pollution-free sterilization system for air inflow and feed supplement of bioreactor |
| CN203695586U (en) * | 2013-12-24 | 2014-07-09 | 欧尔东有限公司 | Device for performing high-temperature steam treatment on medical wastes |
| CN206896531U (en) * | 2017-01-12 | 2018-01-19 | 上海朗锐管道工程有限公司 | A kind of disinfection system of liquid dispensing device |
| CN208727766U (en) * | 2018-08-15 | 2019-04-12 | 河北益存生物科技有限公司 | The sterile pressure-retaining system of disk centrifugal separator |
| CN210230304U (en) * | 2019-06-10 | 2020-04-03 | 上海上药信谊药厂有限公司 | Online sterilization centrifugal system |
-
2019
- 2019-06-10 CN CN201910498563.0A patent/CN110124883A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060009341A1 (en) * | 2004-07-08 | 2006-01-12 | Yoshinori Tobita | Centrifugal separator |
| CN202830031U (en) * | 2012-09-29 | 2013-03-27 | 北京华都诗华生物制品有限公司 | Pollution-free sterilization system for air inflow and feed supplement of bioreactor |
| CN203695586U (en) * | 2013-12-24 | 2014-07-09 | 欧尔东有限公司 | Device for performing high-temperature steam treatment on medical wastes |
| CN206896531U (en) * | 2017-01-12 | 2018-01-19 | 上海朗锐管道工程有限公司 | A kind of disinfection system of liquid dispensing device |
| CN208727766U (en) * | 2018-08-15 | 2019-04-12 | 河北益存生物科技有限公司 | The sterile pressure-retaining system of disk centrifugal separator |
| CN210230304U (en) * | 2019-06-10 | 2020-04-03 | 上海上药信谊药厂有限公司 | Online sterilization centrifugal system |
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